PI0025682 (gene) Melon (PI 482460) v1

Overview
NamePI0025682
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionTRAF-like family protein
Locationchr11: 3423648 .. 3425584 (-)
RNA-Seq ExpressionPI0025682
SyntenyPI0025682
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAATCTCATTGATGACGATATGGACGGTATGGCTCTCTCCACCCTTCCTCACACCATATTAAGCGTTCGTTTTAAAAGCATTTATATTCCTTCAAAAATGTTAATATTATTTTTTACTTTTATTTAATATTTCAATCAAATGCACCTTTGAAGTAGGAATTTGCAAGAAGGTTGAACGAAAATTGAAAACTAGAATGGTTGGACAATATCTACGTCTCATTTTTCATTCTAGGCCAACCAATACCATTCTAATTCTCAGTTTTAACACATTTTAAGAGTTTTATGGTCCAAGAGTTAATTAGGACGTTTGTGAATGGCCAAGTGTAGGGAAGTGTTTTTGAAGTAAAGGACAAAATTCTTCTTTTTTTTTTGGTTGTAATTTACCAAGAATTTATATACACTATAAGGTGTTCCCTGTTCCCTACTGAATGGGAAAGGATTAGCTGTACACAGCACAACGTTAGACTAATTCAATTTCAACATGGAAAAATAGGGAATTGAAGCACTATTGCACTCAGTAATTTTAACAATTTCTATGTTAAAAACGAATGCACTTATCGGAATTCATAAGTTACTGAAGGAAATGCAAGACCATAGCATAGACTTTGTCCTCTTTTGCTTTCTTACCAATACGAAATAATATGCAGAGATCTCAAGATATGTTTCCGATGTCCCGCCTGCTCATTACACAGTCAAGATAGAGTCCTTTTCATTGTTAACAAAGAACTCAGTGGATCGGTTTGAATCCGGGGAGTTTGAAGCAGGTGGCTACAAATGGTAAATGATATTCCTTCAGACTCTTCATCTTCTTTATAAATTTTGAGGCTTGAGCTTGCACTGCCTTGTCATAATCATGTAATGTTCTTGCAGGAAATTGGCACTCCATCCACTTGGTGACAAAAGCAAGAATGGGAATGACCACATCTCTCTCTATTTGGCAATTGCAGGGACCAATTCCCTCCCACCAAGTTGGGAAGTGTTCGTTGTTTACCGACTCTTTTTGCTCGATCAGAACAAGGATAACTACCTCACAGTTGAAGGTATATCTTCCCTTGCCTTCAAGAAATAAGCTGTTGCCTGTATGCAATTTGAGTAACAATATCTGTTAAACGAAGATGGCAAGTGGAAGCCAAGGCGGTTTCGAGGAATAAAGAAAGAATGGGGTTTCGATAAATACATTAACCTAAAAGAATTCAATGAGTCCTCCAATGGGTATCTGGTGGATGATGTGTGTGTGTTTGGAGCTGAGGTCTTTGTCTGCAAAGAAAATTTCAAAGGAGGCAAGGGGGAGTGTTTATCGATGATAAAAAGTCCCGTGAGTTACAAGCATGTTTGGAAAATTGATAACTTCTCCAAGTTAGATGCTCAGAGCTATGAGTCCAAAATCTTCAATGCAGGGGATAAAAAATGGTATCCATCTACTTTTCACACATTCTGATAATTAACTGACCGGGTTTGAAGCTGGTTAATCTGATGACGGGTACCCACACGTATTCATTGTGATGTTGCAGGAAAATTCGAGTGTATCCAAAGGGTCATAGCAGTGGTGAAGGCAGCCATCTTTCCCTATTTCTGGCATTGGCAGATCCCGCTGCGCTGCATCCTGCAACCAAAATATATGCAGAGGTAACGTTGCGCCTGCAAGATCAGATGTACTCAAAGCATAAATCTGGAAAAGGTAAAAATACATTATCACAATGACTCACATGATCACATACAAACTATTCAGTTTTAACACAACGGCAAGGATTTTTTTTTGTTTTGTTGTTGGCAGTTTCATATTGGTTTAGTGCGTCAAACCCAGAAGTTGGAGGGCCAAGATTCATGCTCTTAAACAATTTCATACAACCTAACATGGGTTTTGTGGTGAAGGATGCTTTCATTGTTGAGGCAGAGGTCAACGTCATTGGAGTGGTCAATGCATTCTCTTGA

mRNA sequence

ATGGCCAATCTCATTGATGACGATATGGACGAGATCTCAAGATATGTTTCCGATGTCCCGCCTGCTCATTACACAGTCAAGATAGAGTCCTTTTCATTGTTAACAAAGAACTCAGTGGATCGGTTTGAATCCGGGGAGTTTGAAGCAGGTGGCTACAAATGGAAATTGGCACTCCATCCACTTGGTGACAAAAGCAAGAATGGGAATGACCACATCTCTCTCTATTTGGCAATTGCAGGGACCAATTCCCTCCCACCAAGTTGGGAAGTGTTCGTTGTTTACCGACTCTTTTTGCTCGATCAGAACAAGGATAACTACCTCACAGTTGAAGATGGCAAGTGGAAGCCAAGGCGGTTTCGAGGAATAAAGAAAGAATGGGGTTTCGATAAATACATTAACCTAAAAGAATTCAATGAGTCCTCCAATGGGTATCTGGTGGATGATGTGTGTGTGTTTGGAGCTGAGGTCTTTGTCTGCAAAGAAAATTTCAAAGGAGGCAAGGGGGAGTGTTTATCGATGATAAAAAGTCCCGTGAGTTACAAGCATGTTTGGAAAATTGATAACTTCTCCAAGTTAGATGCTCAGAGCTATGAGTCCAAAATCTTCAATGCAGGGGATAAAAAATGGAAAATTCGAGTGTATCCAAAGGGTCATAGCAGTGGTGAAGGCAGCCATCTTTCCCTATTTCTGGCATTGGCAGATCCCGCTGCGCTGCATCCTGCAACCAAAATATATGCAGAGGTAACGTTGCGCCTGCAAGATCAGATGTACTCAAAGCATAAATCTGGAAAAGTTTCATATTGGTTTAGTGCGTCAAACCCAGAAGTTGGAGGGCCAAGATTCATGCTCTTAAACAATTTCATACAACCTAACATGGGTTTTGTGGTGAAGGATGCTTTCATTGTTGAGGCAGAGGTCAACGTCATTGGAGTGGTCAATGCATTCTCTTGA

Coding sequence (CDS)

ATGGCCAATCTCATTGATGACGATATGGACGAGATCTCAAGATATGTTTCCGATGTCCCGCCTGCTCATTACACAGTCAAGATAGAGTCCTTTTCATTGTTAACAAAGAACTCAGTGGATCGGTTTGAATCCGGGGAGTTTGAAGCAGGTGGCTACAAATGGAAATTGGCACTCCATCCACTTGGTGACAAAAGCAAGAATGGGAATGACCACATCTCTCTCTATTTGGCAATTGCAGGGACCAATTCCCTCCCACCAAGTTGGGAAGTGTTCGTTGTTTACCGACTCTTTTTGCTCGATCAGAACAAGGATAACTACCTCACAGTTGAAGATGGCAAGTGGAAGCCAAGGCGGTTTCGAGGAATAAAGAAAGAATGGGGTTTCGATAAATACATTAACCTAAAAGAATTCAATGAGTCCTCCAATGGGTATCTGGTGGATGATGTGTGTGTGTTTGGAGCTGAGGTCTTTGTCTGCAAAGAAAATTTCAAAGGAGGCAAGGGGGAGTGTTTATCGATGATAAAAAGTCCCGTGAGTTACAAGCATGTTTGGAAAATTGATAACTTCTCCAAGTTAGATGCTCAGAGCTATGAGTCCAAAATCTTCAATGCAGGGGATAAAAAATGGAAAATTCGAGTGTATCCAAAGGGTCATAGCAGTGGTGAAGGCAGCCATCTTTCCCTATTTCTGGCATTGGCAGATCCCGCTGCGCTGCATCCTGCAACCAAAATATATGCAGAGGTAACGTTGCGCCTGCAAGATCAGATGTACTCAAAGCATAAATCTGGAAAAGTTTCATATTGGTTTAGTGCGTCAAACCCAGAAGTTGGAGGGCCAAGATTCATGCTCTTAAACAATTTCATACAACCTAACATGGGTTTTGTGGTGAAGGATGCTTTCATTGTTGAGGCAGAGGTCAACGTCATTGGAGTGGTCAATGCATTCTCTTGA

Protein sequence

MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVIGVVNAFS
Homology
BLAST of PI0025682 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 5.8e-15
Identity = 46/143 (32.17%), Postives = 83/143 (58.04%), Query Frame = 0

Query: 175 KSPVSYKHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALAD 234
           + P + K  W I NFS+ + + + S +F  G  KW+I ++PKG++     HLS++L ++D
Sbjct: 50  EDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKGNNV---DHLSMYLDVSD 109

Query: 235 PAALHPATKIYAEVTLRLQDQMYSKHKSGK-VSYWFSASNPEVGGPRFMLLNNFIQPNMG 294
            A+L      YA+ +L + +Q+++++   K   + F+A   + G   FM L+    P+ G
Sbjct: 110 AASLPYGWSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPSRG 169

Query: 295 FVVKDAFIVEAEVNVIGVVNAFS 317
           ++V D  +VEAEV V  V++ +S
Sbjct: 170 YLVNDTVLVEAEVAVRKVLDYWS 189

BLAST of PI0025682 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.9e-14
Identity = 45/143 (31.47%), Postives = 81/143 (56.64%), Query Frame = 0

Query: 175 KSPVSYKHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALAD 234
           + P S K  W I  F++L+ + + S +F  G  KW+I ++PKG++     HLS++L +AD
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKGNNV---DHLSMYLDVAD 108

Query: 235 PAALHPATKIYAEVTLRLQDQMYSKHKSGK-VSYWFSASNPEVGGPRFMLLNNFIQPNMG 294
            A L      Y++ +L + +Q+ +++   K   + F+A   + G   FM L+   +P  G
Sbjct: 109 AANLPYGWSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPTRG 168

Query: 295 FVVKDAFIVEAEVNVIGVVNAFS 317
           ++V D  ++EAEV V  V++ +S
Sbjct: 169 YLVNDTVLIEAEVAVRKVLDYWS 188

BLAST of PI0025682 vs. ExPASy Swiss-Prot
Match: Q9M2J5 (MATH domain and coiled-coil domain-containing protein At3g58210 OS=Arabidopsis thaliana OX=3702 GN=At3g58210 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 5.5e-13
Identity = 48/136 (35.29%), Postives = 68/136 (50.00%), Query Frame = 0

Query: 178 VSYKHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAA 237
           V  K  W I NFS   ++   S  F  G  KW++ VYP+G +   G HLSLFL +ADP +
Sbjct: 5   VDNKFTWVIQNFSSSQSRVVPSNQFVIGGCKWRLLVYPEGFNK-SGDHLSLFLEVADPRS 64

Query: 238 LHPATKIYAEVTLRLQDQMYSK-HKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVV 297
           L P    +A   L + +Q   K  K  + + WF+   P  G    + L      + GF+V
Sbjct: 65  LPPGWSRHARYLLTIVNQHSDKISKRNEATKWFNQKIPGWGLSAMIPLTKLHAKDGGFLV 124

Query: 298 KDAFIVEAEVNVIGVV 313
            D   + AEVNV+ V+
Sbjct: 125 NDELKIVAEVNVLEVI 139

BLAST of PI0025682 vs. ExPASy Swiss-Prot
Match: Q8T126 (Probable inactive serine/threonine-protein kinase fnkC OS=Dictyostelium discoideum OX=44689 GN=fnkC PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-09
Identity = 66/294 (22.45%), Postives = 118/294 (40.14%), Query Frame = 0

Query: 24   YTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLG-DKSKNGNDHISLYLAIAGTN 83
            + + I +FS    N  D+F S  F   G  W+   +  G D S +G   I +       N
Sbjct: 1028 WIISINNFS----NRKDQFYSPIFSLIGSNWRCKFYSNGKDASTSGKLSIFISNCDLLNN 1087

Query: 84   SLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFDKYINLKEFNESSN 143
                  E  + Y+L L++Q   N       K     F   +   G+  +I L      +N
Sbjct: 1088 PFTIFLEKSISYKLTLINQKNPN---ESIQKSSSHTFSIKEFNHGYGSFIGLFSLLNPNN 1147

Query: 144  GYLVDDVCVFGAEVFVCK------ENFKGGKGECLSMIKSPVSYKHVWKIDNFSKLDAQS 203
            G+LV++      +           + +  G  +  S     +S K            ++ 
Sbjct: 1148 GFLVNNTIKVRIDAAPTSPLVNTYDKYNIGLNQAFSYSVPMMSKK------------SEP 1207

Query: 204  YESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVTLRLQDQM 263
            + S IF +  +KW I++YP G  S   +++S+FL   D    +         +L L  Q+
Sbjct: 1208 FISPIFMSCGRKWIIKIYPMGQPS--SNYMSVFLEYRDEGEEN------VHFSLELISQL 1267

Query: 264  YSKHKSGKVSYW----FSASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEV 307
            Y +     + YW    F++ +   G P+F+ ++  + P+MGF+V D  I+   +
Sbjct: 1268 YPEQ---SIKYWVQYRFNSKSNSFGYPKFIGVSTLMDPDMGFLVNDTIILNVSI 1291

BLAST of PI0025682 vs. ExPASy Swiss-Prot
Match: Q9M2H5 (MATH domain and coiled-coil domain-containing protein At3g58410 OS=Arabidopsis thaliana OX=3702 GN=At3g58410 PE=4 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 2.2e-09
Identity = 41/136 (30.15%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 178 VSYKHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAA 237
           V  K  W I NFS L  + + S  F  GD KW++ +YPKG++     +LSLFL +AD  +
Sbjct: 5   VGKKFAWVIKNFSSLQCKKFYSVPFQIGDCKWRLSIYPKGNNC---DYLSLFLEVADFKS 64

Query: 238 LHPATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVK 297
           L    + Y ++ L +  Q+ +  +  K   WF       G    + L        GF+V 
Sbjct: 65  LPSGWRRYVKLRLYIVKQLSTLIR--KTHRWFDQEMWGWGFLYMLPLTKLHDEKEGFLVN 124

Query: 298 DAFIVEAEVNVIGVVN 314
              ++ AEV+ +G ++
Sbjct: 125 GELMIVAEVDALGFID 135

BLAST of PI0025682 vs. ExPASy TrEMBL
Match: A0A1S3C7I4 (uncharacterized protein LOC103497755 OS=Cucumis melo OX=3656 GN=LOC103497755 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.4e-174
Identity = 298/316 (94.30%), Postives = 306/316 (96.84%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MANLI DDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKL LHP
Sbjct: 1   MANLIGDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
           LGDKSK+GNDHISLYLAIAGT+SL PSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR
Sbjct: 61  LGDKSKSGNDHISLYLAIAGTDSLQPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KKEWGFDKYI+LKEFNESSNGYLVDDVCVFGAEVFVCKE FKGGKGECLSMIKSPV+Y
Sbjct: 121 GMKKEWGFDKYISLKEFNESSNGYLVDDVCVFGAEVFVCKETFKGGKGECLSMIKSPVTY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KHVWKIDNFSKLDA+SYESKIFNAGDKKWKIRVYPKG  SGEGSHLSLFLALAD AALHP
Sbjct: 181 KHVWKIDNFSKLDAESYESKIFNAGDKKWKIRVYPKGRGSGEGSHLSLFLALADSAALHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLRLQDQ+YSKH SGKVSYWFSASNPEVGGPRFMLLNNFIQPN GF+VKDAF
Sbjct: 241 ATKIYAEVTLRLQDQLYSKHHSGKVSYWFSASNPEVGGPRFMLLNNFIQPNTGFLVKDAF 300

Query: 301 IVEAEVNVIGVVNAFS 317
           IVEAEVNVIGV NAFS
Sbjct: 301 IVEAEVNVIGVANAFS 316

BLAST of PI0025682 vs. ExPASy TrEMBL
Match: A0A5D3BTJ2 (Protein GFS12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00700 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.6e-169
Identity = 290/307 (94.46%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 10   DEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGN 69
            DEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKL LHPLGDKSK+GN
Sbjct: 1627 DEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLVLHPLGDKSKSGN 1686

Query: 70   DHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFD 129
            DHISLYLAIAGT+SL PSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRG+KKEWGFD
Sbjct: 1687 DHISLYLAIAGTDSLQPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGMKKEWGFD 1746

Query: 130  KYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNF 189
            KYI+LKEFNESSNGYLVDDVCVFGAEVFVCKE FKGGKGECLSMIKSPV+YKHVWKIDNF
Sbjct: 1747 KYISLKEFNESSNGYLVDDVCVFGAEVFVCKETFKGGKGECLSMIKSPVTYKHVWKIDNF 1806

Query: 190  SKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVT 249
            SKLDA+SYESKIFNAGDKKWKIRVYPKG  SGEGSHLSLFLALAD AALHPATKIYAEVT
Sbjct: 1807 SKLDAESYESKIFNAGDKKWKIRVYPKGRGSGEGSHLSLFLALADSAALHPATKIYAEVT 1866

Query: 250  LRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVI 309
            LRLQDQ+YSKH SGKVSYWFSASNPEVGGPRFMLLNNFIQPN GF+VKDAFIVEAEVNVI
Sbjct: 1867 LRLQDQLYSKHHSGKVSYWFSASNPEVGGPRFMLLNNFIQPNTGFLVKDAFIVEAEVNVI 1926

Query: 310  GVVNAFS 317
            GV NAFS
Sbjct: 1927 GVANAFS 1933

BLAST of PI0025682 vs. ExPASy TrEMBL
Match: A0A0A0K9U9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000670 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.6e-169
Identity = 288/316 (91.14%), Postives = 304/316 (96.20%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MANLI DD+DEISRYVSDVPPAHYTVKIESFSLLTKNSVD+FESGEFEAGGYKWKL LHP
Sbjct: 1   MANLIGDDIDEISRYVSDVPPAHYTVKIESFSLLTKNSVDQFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
            GDKSKNGNDHISLYL IAGT+SL PSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR
Sbjct: 61  HGDKSKNGNDHISLYLEIAGTDSLQPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KK+WGFDKYI+LKEF ESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPV+Y
Sbjct: 121 GMKKQWGFDKYISLKEFKESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVTY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KHVWKIDNFSKLDA+SYESKIFNAGDKKWKIRVYPKGH SGEGSHLS +L LADPAALHP
Sbjct: 181 KHVWKIDNFSKLDAESYESKIFNAGDKKWKIRVYPKGHGSGEGSHLSPYLELADPAALHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLRLQDQ+YSKH SGKVSYWFSASNPEVGGPRF+LL+NF QPN+GF+VKDAF
Sbjct: 241 ATKIYAEVTLRLQDQIYSKHHSGKVSYWFSASNPEVGGPRFILLDNFKQPNIGFLVKDAF 300

Query: 301 IVEAEVNVIGVVNAFS 317
           IVEAEV++IGV NAFS
Sbjct: 301 IVEAEVSIIGVANAFS 316

BLAST of PI0025682 vs. ExPASy TrEMBL
Match: A0A6J1CEA8 (uncharacterized protein LOC111009925 OS=Momordica charantia OX=3673 GN=LOC111009925 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.3e-155
Identity = 264/316 (83.54%), Postives = 290/316 (91.77%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MA+LI  DMDEI+RYVSDVPPAHYTVKIESFSL  KNSVDRFESGEFEAGGYKWKL LHP
Sbjct: 1   MADLIGGDMDEIARYVSDVPPAHYTVKIESFSLFAKNSVDRFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
           LG+KSKN NDHISLYL IAGT+SL  +WEVFVVYRLFLLDQN DNY TVEDGKWKPRRFR
Sbjct: 61  LGNKSKNVNDHISLYLEIAGTDSLQSNWEVFVVYRLFLLDQNNDNYHTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KKEWGFDKYI+LKEFN+SSNGY VDD+CVFGAEVFVCKENFKGG+GECLSMIK+P+ Y
Sbjct: 121 GMKKEWGFDKYISLKEFNDSSNGYAVDDLCVFGAEVFVCKENFKGGRGECLSMIKNPIIY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KH+WKIDNFSKL A+S+ES IFNAG+KKWKIRVYPKG  SGEGSHLSLF+ALADP  LHP
Sbjct: 181 KHIWKIDNFSKLGAESHESAIFNAGEKKWKIRVYPKGRGSGEGSHLSLFVALADPTTLHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLR+QDQ++SKH SGKVSYWF+ASNPEVGG RF+ L+NF QPNMGF+VKD  
Sbjct: 241 ATKIYAEVTLRVQDQVHSKHNSGKVSYWFNASNPEVGGLRFIPLSNFRQPNMGFLVKDVC 300

Query: 301 IVEAEVNVIGVVNAFS 317
           +VEAEV+VIGV NAFS
Sbjct: 301 VVEAEVSVIGVANAFS 316

BLAST of PI0025682 vs. ExPASy TrEMBL
Match: A0A6J1KI45 (uncharacterized protein LOC111494157 OS=Cucurbita maxima OX=3661 GN=LOC111494157 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.5e-154
Identity = 260/316 (82.28%), Postives = 289/316 (91.46%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MANL+ DDMDEI+RYVSDVPPAHYTVKIESFSLL KN VDR+ESGEFEAGGYKWKL +HP
Sbjct: 1   MANLLGDDMDEIARYVSDVPPAHYTVKIESFSLLAKNCVDRYESGEFEAGGYKWKLVVHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
           LG+KSKNG+DHISLYLAIAGT+SL  +WE+FV+YRLFLLDQN DNYLTVEDGK KPRRFR
Sbjct: 61  LGNKSKNGDDHISLYLAIAGTDSLQTNWEMFVIYRLFLLDQNNDNYLTVEDGKMKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KKEWG DKYI+LKEFNESSNGYLVDD CVFGAEVFVCKENFKGGKGECLSM+KSP+ +
Sbjct: 121 GMKKEWGIDKYISLKEFNESSNGYLVDDTCVFGAEVFVCKENFKGGKGECLSMVKSPIIH 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KH+WKIDNFSKLDA+ +ES+IFNAGD+KWK+RVYP G  S +GSHLS FLALADP  LHP
Sbjct: 181 KHIWKIDNFSKLDAECHESRIFNAGDQKWKLRVYPNGRGSCKGSHLSFFLALADPTTLHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLRLQDQ +SKH SGKVSYWF+ASNPEVGG RF+LL+ F QPNMGF++KD  
Sbjct: 241 ATKIYAEVTLRLQDQEHSKHHSGKVSYWFTASNPEVGGLRFILLSKFRQPNMGFLLKDVC 300

Query: 301 IVEAEVNVIGVVNAFS 317
           IVEAEVNV+GV NAFS
Sbjct: 301 IVEAEVNVLGVANAFS 316

BLAST of PI0025682 vs. NCBI nr
Match: XP_008458292.1 (PREDICTED: uncharacterized protein LOC103497755 [Cucumis melo])

HSP 1 Score: 619.8 bits (1597), Expect = 1.3e-173
Identity = 298/316 (94.30%), Postives = 306/316 (96.84%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MANLI DDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKL LHP
Sbjct: 1   MANLIGDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
           LGDKSK+GNDHISLYLAIAGT+SL PSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR
Sbjct: 61  LGDKSKSGNDHISLYLAIAGTDSLQPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KKEWGFDKYI+LKEFNESSNGYLVDDVCVFGAEVFVCKE FKGGKGECLSMIKSPV+Y
Sbjct: 121 GMKKEWGFDKYISLKEFNESSNGYLVDDVCVFGAEVFVCKETFKGGKGECLSMIKSPVTY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KHVWKIDNFSKLDA+SYESKIFNAGDKKWKIRVYPKG  SGEGSHLSLFLALAD AALHP
Sbjct: 181 KHVWKIDNFSKLDAESYESKIFNAGDKKWKIRVYPKGRGSGEGSHLSLFLALADSAALHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLRLQDQ+YSKH SGKVSYWFSASNPEVGGPRFMLLNNFIQPN GF+VKDAF
Sbjct: 241 ATKIYAEVTLRLQDQLYSKHHSGKVSYWFSASNPEVGGPRFMLLNNFIQPNTGFLVKDAF 300

Query: 301 IVEAEVNVIGVVNAFS 317
           IVEAEVNVIGV NAFS
Sbjct: 301 IVEAEVNVIGVANAFS 316

BLAST of PI0025682 vs. NCBI nr
Match: TYK02983.1 (protein GFS12 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 605.1 bits (1559), Expect = 3.4e-169
Identity = 290/307 (94.46%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 10   DEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGN 69
            DEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKL LHPLGDKSK+GN
Sbjct: 1627 DEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLVLHPLGDKSKSGN 1686

Query: 70   DHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFD 129
            DHISLYLAIAGT+SL PSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRG+KKEWGFD
Sbjct: 1687 DHISLYLAIAGTDSLQPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGMKKEWGFD 1746

Query: 130  KYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNF 189
            KYI+LKEFNESSNGYLVDDVCVFGAEVFVCKE FKGGKGECLSMIKSPV+YKHVWKIDNF
Sbjct: 1747 KYISLKEFNESSNGYLVDDVCVFGAEVFVCKETFKGGKGECLSMIKSPVTYKHVWKIDNF 1806

Query: 190  SKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVT 249
            SKLDA+SYESKIFNAGDKKWKIRVYPKG  SGEGSHLSLFLALAD AALHPATKIYAEVT
Sbjct: 1807 SKLDAESYESKIFNAGDKKWKIRVYPKGRGSGEGSHLSLFLALADSAALHPATKIYAEVT 1866

Query: 250  LRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVI 309
            LRLQDQ+YSKH SGKVSYWFSASNPEVGGPRFMLLNNFIQPN GF+VKDAFIVEAEVNVI
Sbjct: 1867 LRLQDQLYSKHHSGKVSYWFSASNPEVGGPRFMLLNNFIQPNTGFLVKDAFIVEAEVNVI 1926

Query: 310  GVVNAFS 317
            GV NAFS
Sbjct: 1927 GVANAFS 1933

BLAST of PI0025682 vs. NCBI nr
Match: XP_004138521.1 (uncharacterized protein LOC101206506 [Cucumis sativus])

HSP 1 Score: 605.1 bits (1559), Expect = 3.4e-169
Identity = 288/316 (91.14%), Postives = 304/316 (96.20%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MANLI DD+DEISRYVSDVPPAHYTVKIESFSLLTKNSVD+FESGEFEAGGYKWKL LHP
Sbjct: 1   MANLIGDDIDEISRYVSDVPPAHYTVKIESFSLLTKNSVDQFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
            GDKSKNGNDHISLYL IAGT+SL PSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR
Sbjct: 61  HGDKSKNGNDHISLYLEIAGTDSLQPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KK+WGFDKYI+LKEF ESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPV+Y
Sbjct: 121 GMKKQWGFDKYISLKEFKESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVTY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KHVWKIDNFSKLDA+SYESKIFNAGDKKWKIRVYPKGH SGEGSHLS +L LADPAALHP
Sbjct: 181 KHVWKIDNFSKLDAESYESKIFNAGDKKWKIRVYPKGHGSGEGSHLSPYLELADPAALHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLRLQDQ+YSKH SGKVSYWFSASNPEVGGPRF+LL+NF QPN+GF+VKDAF
Sbjct: 241 ATKIYAEVTLRLQDQIYSKHHSGKVSYWFSASNPEVGGPRFILLDNFKQPNIGFLVKDAF 300

Query: 301 IVEAEVNVIGVVNAFS 317
           IVEAEV++IGV NAFS
Sbjct: 301 IVEAEVSIIGVANAFS 316

BLAST of PI0025682 vs. NCBI nr
Match: XP_038875826.1 (uncharacterized protein LOC120068190 [Benincasa hispida])

HSP 1 Score: 570.5 bits (1469), Expect = 9.2e-159
Identity = 274/316 (86.71%), Postives = 296/316 (93.67%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MA+LI D  DEISRYVSDVPPAHYTVKIESFSLLTKN+VDRFESGEFEAGGYKWKL LHP
Sbjct: 1   MADLIGD--DEISRYVSDVPPAHYTVKIESFSLLTKNNVDRFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
            G+KSKNGN+HISLYLAIAGT+SL PSWEVFVVYRLFLLDQN DNYLTVEDGKWKPRRFR
Sbjct: 61  HGNKSKNGNNHISLYLAIAGTDSLQPSWEVFVVYRLFLLDQNNDNYLTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KKEWGFDKYI+L+EFNESSNGYLVDDVCVFGAEVFVCKE+FKGGKGECLSMIKSP+ Y
Sbjct: 121 GLKKEWGFDKYISLEEFNESSNGYLVDDVCVFGAEVFVCKEHFKGGKGECLSMIKSPIIY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KH+WKI+NFSKLDA+SYESKIFN  D+KWKIRVYP+G  S +GSHLSLF+ALADPAALHP
Sbjct: 181 KHIWKIENFSKLDAESYESKIFNGADQKWKIRVYPQGQGSVKGSHLSLFVALADPAALHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLRLQDQ+ SKH SGKVSYWFSASNPE+GG RF+LL+NF QPNMGF+VKD  
Sbjct: 241 ATKIYAEVTLRLQDQVQSKHHSGKVSYWFSASNPEIGGLRFILLSNFKQPNMGFLVKDIC 300

Query: 301 IVEAEVNVIGVVNAFS 317
           IVEAEVNVIGV NAFS
Sbjct: 301 IVEAEVNVIGVANAFS 314

BLAST of PI0025682 vs. NCBI nr
Match: XP_022138858.1 (uncharacterized protein LOC111009925 [Momordica charantia])

HSP 1 Score: 558.9 bits (1439), Expect = 2.8e-155
Identity = 264/316 (83.54%), Postives = 290/316 (91.77%), Query Frame = 0

Query: 1   MANLIDDDMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHP 60
           MA+LI  DMDEI+RYVSDVPPAHYTVKIESFSL  KNSVDRFESGEFEAGGYKWKL LHP
Sbjct: 1   MADLIGGDMDEIARYVSDVPPAHYTVKIESFSLFAKNSVDRFESGEFEAGGYKWKLVLHP 60

Query: 61  LGDKSKNGNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFR 120
           LG+KSKN NDHISLYL IAGT+SL  +WEVFVVYRLFLLDQN DNY TVEDGKWKPRRFR
Sbjct: 61  LGNKSKNVNDHISLYLEIAGTDSLQSNWEVFVVYRLFLLDQNNDNYHTVEDGKWKPRRFR 120

Query: 121 GIKKEWGFDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSY 180
           G+KKEWGFDKYI+LKEFN+SSNGY VDD+CVFGAEVFVCKENFKGG+GECLSMIK+P+ Y
Sbjct: 121 GMKKEWGFDKYISLKEFNDSSNGYAVDDLCVFGAEVFVCKENFKGGRGECLSMIKNPIIY 180

Query: 181 KHVWKIDNFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHP 240
           KH+WKIDNFSKL A+S+ES IFNAG+KKWKIRVYPKG  SGEGSHLSLF+ALADP  LHP
Sbjct: 181 KHIWKIDNFSKLGAESHESAIFNAGEKKWKIRVYPKGRGSGEGSHLSLFVALADPTTLHP 240

Query: 241 ATKIYAEVTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAF 300
           ATKIYAEVTLR+QDQ++SKH SGKVSYWF+ASNPEVGG RF+ L+NF QPNMGF+VKD  
Sbjct: 241 ATKIYAEVTLRVQDQVHSKHNSGKVSYWFNASNPEVGGLRFIPLSNFRQPNMGFLVKDVC 300

Query: 301 IVEAEVNVIGVVNAFS 317
           +VEAEV+VIGV NAFS
Sbjct: 301 VVEAEVSVIGVANAFS 316

BLAST of PI0025682 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.1e-101
Identity = 169/307 (55.05%), Postives = 229/307 (74.59%), Query Frame = 0

Query: 8   DMDEISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKN 67
           D DEI + +SD PP HY VKIESFSLLTK++++R+E+  FEAGGYKWKL L+P G+KSKN
Sbjct: 6   DYDEIIKSISDAPPTHYMVKIESFSLLTKHAIERYETESFEAGGYKWKLVLYPNGNKSKN 65

Query: 68  GNDHISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWG 127
             DH+S+YL++A ++SL P WEV+ V+RL+LLDQNKDNYL ++  +   RRF  +K+EWG
Sbjct: 66  TKDHVSVYLSLADSSSLSPGWEVYAVFRLYLLDQNKDNYLILQGNE---RRFHSVKREWG 125

Query: 128 FDKYINLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKID 187
           FDK+I    F+++SNGYL++D C+FGA+VFV KE  + G+GECLSMIK   S KHVWKI+
Sbjct: 126 FDKFIPTGTFSDASNGYLMEDTCMFGADVFVSKER-RSGRGECLSMIKDATSSKHVWKIE 185

Query: 188 NFSKLDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAE 247
           NFSKLD +SY+S  F AGD+KWKI  YP G   G G+HLS++L L DP  +   TKI+ E
Sbjct: 186 NFSKLDKESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVE 245

Query: 248 VTLRLQDQMYSKHKSGKVSYWFSASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVN 307
            T+R+ DQ+  +H +GKV+ WFS S+ E G  +++ +  F QPN G ++KD  +VEA+V 
Sbjct: 246 FTIRIFDQLQGRHIAGKVTKWFSRSSSEHGWVKYVSMVYFTQPNSGLLLKDVCLVEADVC 305

Query: 308 VIGVVNA 315
           V G+ +A
Sbjct: 306 VHGITSA 308

BLAST of PI0025682 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-46
Identity = 113/306 (36.93%), Postives = 161/306 (52.61%), Query Frame = 0

Query: 12  ISRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGNDH 71
           ++R + +  P+   V I SFS++ K   + +ES  FEA GYKW+L L+  G+K+  GNDH
Sbjct: 50  VTRELREERPSSKIVTITSFSVI-KGRGEPYESSVFEAAGYKWRLVLYVNGNKNDGGNDH 109

Query: 72  ISLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFDKY 131
           ISLY  I  TNSLP  WEV V  +LF+ +     YLTV DG  K  R+   KKEWGF + 
Sbjct: 110 ISLYARIEETNSLPLGWEVNVDLKLFVHNGKLHKYLTVTDGLVK--RYNNAKKEWGFGQL 169

Query: 132 INLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNFSK 191
           I    F  ++ GYL  D   FGAE+F+ K      + E ++ I +P +    WKI  FS 
Sbjct: 170 IPRSTFYNANEGYLDQDTGSFGAEIFIVK---PAQQQEKVTFISNPPNNVFTWKILRFST 229

Query: 192 LDAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVTLR 251
           L+ + Y S  F   D+ W++   PKG   G    L +FL      A   AT  +  V LR
Sbjct: 230 LEDKFYYSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAVNLR 289

Query: 252 LQDQMYSKHKSGKVSYWFS-ASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVIG 311
           L++Q  + H+    + W+   S   VG    +LL +    + G++V DA I EAE+  + 
Sbjct: 290 LKNQRSTNHRQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMVKVS 349

Query: 312 VVNAFS 317
           + N  S
Sbjct: 350 ITNIVS 349

BLAST of PI0025682 vs. TAIR 10
Match: AT5G26260.1 (TRAF-like family protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.3e-46
Identity = 112/305 (36.72%), Postives = 160/305 (52.46%), Query Frame = 0

Query: 13  SRYVSDVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGNDHI 72
           +R + D  P+   V I SFS++ K+  + +ES  FEA GYKW+L L+  G+     N+HI
Sbjct: 52  TRELRDERPSSKIVTITSFSVI-KDRGEPYESSIFEAAGYKWRLVLYVKGNPKGGINNHI 111

Query: 73  SLYLAIAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFDKYI 132
           SLY  I  T +LP  WEV V  +LF+ ++    YL+V DG  K  R+   KKEWGF + I
Sbjct: 112 SLYARIEETETLPRGWEVNVDLKLFVHNRKLKKYLSVTDGTVK--RYNDAKKEWGFTQLI 171

Query: 133 NLKEFNESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNFSKL 192
           +L  F  ++ GYLV D   FGAE+F+        K E ++ I +P      WKI  FS L
Sbjct: 172 SLPTFYNANEGYLVQDTASFGAEIFIVNPT---EKQEKVTFISNPPDNVFTWKILRFSTL 231

Query: 193 DAQSYESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVTLRL 252
           + + Y S  F  GD+ W++   PKG   G    L +FL      A    T  +  V LRL
Sbjct: 232 EDKFYYSDDFLVGDRYWRLGFNPKGSGGGRPHALPIFLYAQGHKANAVVTNTWGAVNLRL 291

Query: 253 QDQMYSKHKSGKVSYWFS-ASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVIGV 312
           ++Q  S HK    + W+   S+  VG    +L++     + G++V DA I EAE+  + V
Sbjct: 292 KNQRSSNHKQLYSAAWYPIRSDYGVGVNNIILMSELKDASKGYMVNDAIIFEAEMVKVSV 350

Query: 313 VNAFS 317
            N  S
Sbjct: 352 TNIVS 350

BLAST of PI0025682 vs. TAIR 10
Match: AT2G04170.1 (TRAF-like family protein )

HSP 1 Score: 176.4 bits (446), Expect = 3.6e-44
Identity = 107/297 (36.03%), Postives = 158/297 (53.20%), Query Frame = 0

Query: 18  DVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGNDHISLYLA 77
           D  P++  + I SFS++ K   + +ES  FEAGGYKW+L L+  G+++  GN+HISLY+ 
Sbjct: 126 DERPSNKILTITSFSVI-KGRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHISLYVR 185

Query: 78  IAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFDKYINLKEF 137
           I  T SLP  WEV V  +LF+ +  +  YL V+DG  K  R+   KKEWG+ K I L  F
Sbjct: 186 IEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVK--RYNDAKKEWGYGKLIPLTTF 245

Query: 138 NESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNFSKLDAQSY 197
            +++ GYL  D+  FGAE+F           E ++ I +P +    WKI +FS L+ + Y
Sbjct: 246 LDTNEGYLEQDIASFGAEIF---SGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 305

Query: 198 ESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVTLRLQDQMY 257
            S  F   D+ W++   PKG   G    + +FL          AT  +  V LRL++Q  
Sbjct: 306 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 365

Query: 258 SKHKSGKVSYWF-SASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVIGVVN 314
           S H     + W+ + S+  VG    + L  F   + G+ V D+ I EAE+  + V N
Sbjct: 366 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTN 416

BLAST of PI0025682 vs. TAIR 10
Match: AT2G04170.2 (TRAF-like family protein )

HSP 1 Score: 176.4 bits (446), Expect = 3.6e-44
Identity = 107/297 (36.03%), Postives = 158/297 (53.20%), Query Frame = 0

Query: 18  DVPPAHYTVKIESFSLLTKNSVDRFESGEFEAGGYKWKLALHPLGDKSKNGNDHISLYLA 77
           D  P++  + I SFS++ K   + +ES  FEAGGYKW+L L+  G+++  GN+HISLY+ 
Sbjct: 126 DERPSNKILTITSFSVI-KGRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHISLYVR 185

Query: 78  IAGTNSLPPSWEVFVVYRLFLLDQNKDNYLTVEDGKWKPRRFRGIKKEWGFDKYINLKEF 137
           I  T SLP  WEV V  +LF+ +  +  YL V+DG  K  R+   KKEWG+ K I L  F
Sbjct: 186 IEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVK--RYNDAKKEWGYGKLIPLTTF 245

Query: 138 NESSNGYLVDDVCVFGAEVFVCKENFKGGKGECLSMIKSPVSYKHVWKIDNFSKLDAQSY 197
            +++ GYL  D+  FGAE+F           E ++ I +P +    WKI +FS L+ + Y
Sbjct: 246 LDTNEGYLEQDIASFGAEIF---SGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 305

Query: 198 ESKIFNAGDKKWKIRVYPKGHSSGEGSHLSLFLALADPAALHPATKIYAEVTLRLQDQMY 257
            S  F   D+ W++   PKG   G    + +FL          AT  +  V LRL++Q  
Sbjct: 306 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 365

Query: 258 SKHKSGKVSYWF-SASNPEVGGPRFMLLNNFIQPNMGFVVKDAFIVEAEVNVIGVVN 314
           S H     + W+ + S+  VG    + L  F   + G+ V D+ I EAE+  + V N
Sbjct: 366 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTN 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FPT15.8e-1532.17Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 ... [more]
Q84WU24.9e-1431.47Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 ... [more]
Q9M2J55.5e-1335.29MATH domain and coiled-coil domain-containing protein At3g58210 OS=Arabidopsis t... [more]
Q8T1261.6e-0922.45Probable inactive serine/threonine-protein kinase fnkC OS=Dictyostelium discoide... [more]
Q9M2H52.2e-0930.15MATH domain and coiled-coil domain-containing protein At3g58410 OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A1S3C7I46.4e-17494.30uncharacterized protein LOC103497755 OS=Cucumis melo OX=3656 GN=LOC103497755 PE=... [more]
A0A5D3BTJ21.6e-16994.46Protein GFS12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0K9U91.6e-16991.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000670 PE=4 SV=1[more]
A0A6J1CEA81.3e-15583.54uncharacterized protein LOC111009925 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A6J1KI452.5e-15482.28uncharacterized protein LOC111494157 OS=Cucurbita maxima OX=3661 GN=LOC111494157... [more]
Match NameE-valueIdentityDescription
XP_008458292.11.3e-17394.30PREDICTED: uncharacterized protein LOC103497755 [Cucumis melo][more]
TYK02983.13.4e-16994.46protein GFS12 isoform X1 [Cucumis melo var. makuwa][more]
XP_004138521.13.4e-16991.14uncharacterized protein LOC101206506 [Cucumis sativus][more]
XP_038875826.19.2e-15986.71uncharacterized protein LOC120068190 [Benincasa hispida][more]
XP_022138858.12.8e-15583.54uncharacterized protein LOC111009925 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G17380.11.1e-10155.05TRAF-like family protein [more]
AT5G26280.11.3e-4636.93TRAF-like family protein [more]
AT5G26260.12.3e-4636.72TRAF-like family protein [more]
AT2G04170.13.6e-4436.03TRAF-like family protein [more]
AT2G04170.23.6e-4436.03TRAF-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002083MATH/TRAF domainSMARTSM00061math_3coord: 181..287
e-value: 8.2E-10
score: 48.6
coord: 26..137
e-value: 1.3E-8
score: 44.7
IPR002083MATH/TRAF domainPFAMPF00917MATHcoord: 29..158
e-value: 6.8E-14
score: 52.0
coord: 186..308
e-value: 2.1E-13
score: 50.4
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 21..156
score: 25.607121
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 179..306
score: 29.774948
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 181..306
e-value: 4.32394E-27
score: 100.917
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 23..156
e-value: 4.12292E-26
score: 98.6054
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 7..163
e-value: 6.3E-39
score: 135.2
coord: 172..312
e-value: 5.6E-33
score: 115.9
NoneNo IPR availablePANTHERPTHR46162TRAF-LIKE FAMILY PROTEINcoord: 7..313
NoneNo IPR availablePANTHERPTHR46162:SF29SUBFAMILY NOT NAMEDcoord: 7..313
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 177..311
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 19..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025682.1PI0025682.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
molecular_function GO:0004672 protein kinase activity