PI0025655 (gene) Melon (PI 482460) v1

Overview
NamePI0025655
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionvesicle-associated protein 2-2-like
Locationchr02: 1230971 .. 1235048 (+)
RNA-Seq ExpressionPI0025655
SyntenyPI0025655
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACGAGGCTTTTGGAAATTCATCCTCTTGAATTGAAGTTCATCTGTAAGTCAATAGAATGAATTATTTTCTGTCTTTTAGACTTTTGTTTTGTTGTTTTCAATGAGCTGTTCATTGATTTTCTGTGGAAACATTAGGAGATTAGTTGCTGATCTCTTTTCTTCCTATGAACCGTTCGATCTTTTGTGGCAAAAGCAAAGTGATTTACTTGTTTGGGTTTTGTTTTAATCCTTCTTTAGTTTTTGAAATTTATGTTTTAATTCTTTCATTTGTACTTTGTATGCTAATTGAGCACATATGAAGTGTATAAAAGTGCCTTTGAATAACTAAAACATTTTTCAAATTTTTGTTGGTAATTTGGTTGTATAGATAGGACAACATGGTTTTTACAATTTTGAAAAATACTTAAATGGTAGTAATTGGTGGAGCGCTTTGATTTAGGTTTGAAATGTGTTTTGGACCTTATGCTTTTAGGTGAAACACATTTTTATAAGCACTTTTGATAACGAACACTAATTAAATGCTTACTTTTGAAGTAAACAAACTTTTCAGTATTTAAAAAAATTTTAAAAGCACTTTTGACTTGATCAGTCGCCAGAAGGATTTGTGATTAGTTTAAAGTACTTTTTACTCTTCTAAAAACGATATTAAACTCAACATTTGTGCTTTTCCTGGAAGTGCTTATTAAAATAGTGTTCTTAGAACTTAAAGTTATCCCCGGCTGAAGTTTTGATGTATGATTTTTTATGTTTTCTGCTAGGGATTTTATTTGCTAAGGTACCTCAAATGAAGTGTCGTATATGCTTTTCTGAATGTATTTTCTCATTTGCTCTTAGGAGATTGTTTACTTTGGACTTATTTTCCACTTCAATTTATCATTCACTCCCTTTTCCAAATTAGAACTAATATTGTTCATAGTTTTCTTGTATGGCCGCCAGTTGAACTAAATAAAGATAGCTTGTGCTCAATTCAGCTAACAAATAAGACAGTAGACCATGTTGCTTTTAAGGTATGGATTACCAATTTTCCTCTCTGTCCTTGTCTCATTTTCTCTCTCTCTTGTACTTCTCAAAAACCAAAATCAAACTGATTTATGATTCATTATGTAGAGATAGCATGTTTTATTGCATGCGAGGTAATGGACAAAACTTCTTTTTTCAGGTTAAGATCACATCTCCAAAGAAGTACTTTGTCCGACCAAAGGTTGGAATCATCGAGCCAAACTCAACTCGTGAATTCACGGGTATTTACTTTTGACTAGTTTAGGCTTTATATTTTCTCTCATGCTTGTTTCAGGAGGGGAAGAGAGGATGTATGGGTGGTATATTTATTTCTAGATGCTGCTAAGTTGTAGTCATGAGGGATCTCTTATCTTAAATAGCGTTAATGAGGCCCCTTTGTGGCTACATTCTTCTGCTTCTAGCACCTTTGCTTGATTAGCTTTATCATCATTTTTTTCTATGCTTTCCTTTCATGTTGTAGTCTATTTGGTGCACCTGTCTTATCTTAATCTATTTATTTTAACATCAATGTGTGAGGCCTTCAATCACGAAACAATATTCTAGAAAGGGGAGTAAGAGGAAATTACTCTCTACAAGTTAGTTACGTAATGCAGAATAGGAAAGCCCAGAAGAGGTGTCCACTAAGAAATGTGGGCTATGTATACGTCCGGGAGAGTGAGGAAATGTTTCTTTCTATGCTTGAATTTTCTGTTGCAAGAGAGGAGGGAAGGAGAGATTACTCGACCTCTCTAATCGGCTGGGAGATTTTTTATCGCCTCAAGGGTTAACTTTCCTCTGTTTATAGTAAGCTTGATACCTTACACAATGATTGGCTCAAGTGCTTGCAACAATCATGGGCTATCCTCAGTCTTGTCATATTGCTCTCATTCTTTCATTATCTCTCTCTCTTTCTTTTCATGTCCTGCACTTGCCTATAACATTTCTTGCTTTCCAAAATTTATATTATGGTTCTTAATTTAGTTAGGATGCAAGGACAACAGCTGGCCCCACCTGATATGCTATGCAAAGACAAGTTCTTAATCCAGAGCACTGTAGTTCCTGCTGATACAACTGATGGGGATATAATCTCTAGCTTGGTAAATGGTTCTACTAGTATTTATTATGCATAATTGGCTATTTGTCTTGGAATTTAACATTTTCTTTTTCTTAAATTTCAGTTTGCCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAATCCTTGTTACCCCATCCATTTCTCCAGTGTTTTCCCAGTTGATGAATCACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGACCAGGAACCTTTAAACAAAAACCATGTAGTTTCTCAACACATGCCCTTTTTGTATGCTTGCTTTAAAATACATTTACTTTTATCTCAAGTTGATTAGGATACTAAGATAAGCAATCGTGACAGAGTGTTTCTGAGAACACATTGGAAGCTTAGAACATGAAAACTGTGCAACCATAGAAACTTGGCACACGACAATAGCTAAATGTTTTGTTGACTTTGTGATGAAATGCATATCTCTTCCATTCTAGCTATAACCAATTTTCTTTGCTTGTCGAATTCACAAAATTTTGGTTGTCTTGCATATTGACCAGGTTCCTGACAATAATACAGAGCCTAGAGATGTCGTTGAAAGGAGCCAGAACCAGCAAGTTTGGCTAACGATTTCATAGATAGCAATGAGGATGATGCGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAGCTCGAGTTAAAGCTCTGTCAGGTCAGTCTTTCGTACAAATATTGATCCAATATTATGAACTCTGAGCATAACGATGAAGTGGAAAAACTGTCCAAAAACCTCTTTTTACTGTTAACTGAGAAAGTTCTGCTAAACGGTGATGTGTATTGATAAACTTTATTTACAGGCTCAAAATGCCATTTCAAAGTTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTCTTGCAGGAGAATTTCGTAATTTTCTGTACCATTCTATTTTCTTCTTGAGCGTATAATTCTTACCACCATCACAAGACCGATGCCGTTTATTGTTGCCTATGTTCTATCATGAAAGGATATCTGAAGACTGTTTATACCCCTCGTTAGGAAACATTAAAATTATTAGTATTATCATTAGCAAGGGCATATTAGTAAATAACTAGTAAGTTTGTTAGGGCTTTTAGTCATAAATAGAGGGAGTGGGGTTGAAAGAAAGGTTTGAAAAGTTTGAAGTAATTTCCAATGGGGAATTTGGGAGAAAGTAGCTCTCTCGAAAGACTGATATATTGTAGTTATTCAGTGATATTGCAATATATATTTCTATTGTTTAGTGTTCTCGGTATTCTTTATTGGTCTAAAGGGTTCTTGTTAGAGGTATCCTAACACTTTCTTTCAGTGTTCTTCTATTGAAGAAATCAGATCGGGAGTTACAAATTTAGTGAATGAGTGATATTTAGTGGATAAAATTTTGGCTGCCATAGCAGAATATGGTACACAAAGGACTAGGGGAGTGTATAATAGGAGAAAATAAGTGTCATACTCTATTAAAGGGATTTGATGTTACATTGCTTCAACTAGATAGTACACTAAGCATATTGCTTTTTTTTTTTTTCTAGATGTGCACTTCTGTCCCAGTCATTTAGTCAAATGGCATTAAATAAAAGAACAGTTTCAAAGTCCAGTTCAGATGCATAACTGAAACATTTAAAAGACTGAATTAAAAATTGAACTATGAAATACTATAGTAAAATCTGTTACGTTTCGAGGACGGTTTGTTTCATCCATTTTTTGTTGGATTCTTGCTAAATTAGACATCTGATTGGCAATATGATTATATGTTCTAGTTTTATAGCTGCAAATTTGTCAAATATGATTCTTTTTTAATTTCAAATTCCTGAAGTCTCTATATGCATTTTCTAAATTTGCAGTCAGAGTTGAGGAAGACAGAGCTCAACAGAGTTCAAGTTGGCTTTCCTGTTTTGTATCTCTACATGGTGGCACTTGTATGTACTTTCCTAGGACGCCTCTTGAGGTAG

mRNA sequence

ATGGAGACGAGGCTTTTGGAAATTCATCCTCTTGAATTGAAGTTCATCTTTGAACTAAATAAAGATAGCTTGTGCTCAATTCAGCTAACAAATAAGACAGTAGACCATGTTGCTTTTAAGGTTAAGATCACATCTCCAAAGAAGTACTTTGTCCGACCAAAGGTTGGAATCATCGAGCCAAACTCAACTCGTGAATTCACGGTTAGGATGCAAGGACAACAGCTGGCCCCACCTGATATGCTATGCAAAGACAAGTTCTTAATCCAGAGCACTGTAGTTCCTGCTGATACAACTGATGGGGATATAATCTCTAGCTTGTTTGCCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAATCCTTGTTACCCCATCCATTTCTCCAGTGTTTTCCCAGTTGATGAATCACCAAATAATCTATGAGCCAGAACCAGCAAGTTTGGCTAACGATTTCATAGATAGCAATGAGGATGATGCGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAGCTCGAGTTAAAGCTCTGTCAGGCTCAAAATGCCATTTCAAAGTTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTCTTGCAGGAGAATTTCTCAGAGTTGAGGAAGACAGAGCTCAACAGAGTTCAAGTTGGCTTTCCTGTTTTGTATCTCTACATGGTGGCACTTGTATGTACTTTCCTAGGACGCCTCTTGAGGTAG

Coding sequence (CDS)

ATGGAGACGAGGCTTTTGGAAATTCATCCTCTTGAATTGAAGTTCATCTTTGAACTAAATAAAGATAGCTTGTGCTCAATTCAGCTAACAAATAAGACAGTAGACCATGTTGCTTTTAAGGTTAAGATCACATCTCCAAAGAAGTACTTTGTCCGACCAAAGGTTGGAATCATCGAGCCAAACTCAACTCGTGAATTCACGGTTAGGATGCAAGGACAACAGCTGGCCCCACCTGATATGCTATGCAAAGACAAGTTCTTAATCCAGAGCACTGTAGTTCCTGCTGATACAACTGATGGGGATATAATCTCTAGCTTGTTTGCCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAATCCTTGTTACCCCATCCATTTCTCCAGTGTTTTCCCAGTTGATGAATCACCAAATAATCTATGAGCCAGAACCAGCAAGTTTGGCTAACGATTTCATAGATAGCAATGAGGATGATGCGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAGCTCGAGTTAAAGCTCTGTCAGGCTCAAAATGCCATTTCAAAGTTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTCTTGCAGGAGAATTTCTCAGAGTTGAGGAAGACAGAGCTCAACAGAGTTCAAGTTGGCTTTCCTGTTTTGTATCTCTACATGGTGGCACTTGTATGTACTTTCCTAGGACGCCTCTTGAGGTAG

Protein sequence

METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEPNSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKLGVILVTPSISPVFSQLMNHQIIYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR
Homology
BLAST of PI0025655 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.8e-38
Identity = 128/385 (33.25%), Postives = 164/385 (42.60%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M   LL+I P  L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
            ST EFTV MQ  +  PPDM+CKDKFLIQST V A+TTD DI +S+F+K  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVILVTPSISPVFSQL-----------------------------MNHQIIYEP------ 180
            V LV PS SP  S +                                +I+ EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------EPASLANDF----------------------------- 240
                                 E    AND                              
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 -------------------------------IDSNE----------------------DD 246
                                          +DS                        D+
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of PI0025655 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.8e-33
Identity = 97/243 (39.92%), Postives = 135/243 (55.56%), Query Frame = 0

Query: 5   LLEIHPLELKFIFELNKDSLCSIQLTNKTVDH-VAFKVKITSPKKYFVRPKVGIIEPNST 64
           L+ IHP ELKF FEL K S CS+QLTNKT    VAFKVK T+P+KY VRP  G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  REFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKLGVI 124
              TV MQ Q+ AP DM CKDKFL+Q+ VV   TT  ++++ +F KE G+ IE+ KL V+
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LVTPS-ISPVFSQLMNHQIIYEPEPASLANDFIDSNEDDAEMIKHL-EELKLKLSELELK 184
            +  +  SPV            PE +   N  + S  D A     L +++     E   K
Sbjct: 126 YIPANPPSPV------------PEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSEK 185

Query: 185 LCQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLG 244
             +A + ISKL EEK  + Q+++ L+     LRK    +   G  +L + +V L+   +G
Sbjct: 186 SSEAWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIG 236

BLAST of PI0025655 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.8e-33
Identity = 91/243 (37.45%), Postives = 126/243 (51.85%), Query Frame = 0

Query: 3   TRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEPNS 62
           + LL + PL+L+F FEL K   CS+ LTNKT ++VAFKVK T+PKKY VRP  G++ P S
Sbjct: 20  SELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRS 79

Query: 63  TREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKLGV 122
           T E  V MQ Q+ AP DM CKDKFL+Q  +     T  ++   +F+KE G  +EE KL V
Sbjct: 80  TCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRV 139

Query: 123 ILVTPSISPVFSQLMNHQIIYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKL 182
             V P   P         +    E  S     +  N   +E       +  K    E   
Sbjct: 140 TYVAPPRPP-------SPVHEGSEEGSSPRASVSDNGHGSEFSFERFIVDNKAGHQE-NT 199

Query: 183 CQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGR 242
            +A+  I+KL EEKQ +IQ    LQ    +LR+        G P +Y+ +V L+   LG 
Sbjct: 200 SEARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGY 254

Query: 243 LLR 246
           +++
Sbjct: 260 IMK 254

BLAST of PI0025655 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-32
Identity = 89/250 (35.60%), Postives = 131/250 (52.40%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M   LL I P++L+F FEL K   CS+ L NKT ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
            S+ E  V MQ Q+ AP D+ CKDKFL+Q  V     T  D+   +F+KE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVILVTPSISPVFSQLMNHQIIYEPEPASL-----ANDFIDSNEDDAEMIKHLEELKLKL 180
            V+ V P   P  S +           AS+     A+DF  +    A+ +          
Sbjct: 121 RVVYVAPPRPP--SPVREGSEEGSSPRASVSDNGNASDFTAAPRFSADRV---------- 180

Query: 181 SELELKLCQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVAL 240
            + +    +A+  ++KL EEK  ++Q    LQ+   +LR+        G P +Y+ +V L
Sbjct: 181 -DAQDNSSEARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGL 237

Query: 241 VCTFLGRLLR 246
           +   LG +++
Sbjct: 241 IGLILGYIMK 237

BLAST of PI0025655 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.5e-30
Identity = 85/245 (34.69%), Postives = 126/245 (51.43%), Query Frame = 0

Query: 2   ETRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEPN 61
           E +L+ I P ELKF+FEL K S C +++ NKT ++VAFKVK TSPKKYFVRP  G+I+P 
Sbjct: 6   ENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPW 65

Query: 62  STREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKLG 121
            +    V +Q Q+  PPDM CKDKFL+QST+VP  T   ++    F K+ GK + E KL 
Sbjct: 66  DSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLK 125

Query: 122 VILVTPSISPVFSQLMNHQIIYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELK 181
           V  +TPS +   S+                +   + +   +E I                
Sbjct: 126 VSYITPSTTQRSSE----------------SGATNGDGQSSETI---------------- 185

Query: 182 LCQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQ-VGFPVLYLYMVALVCTFL 241
                + I +LKEE+  ++++T+ LQ     +R+    R    G  +    MV L+   +
Sbjct: 186 -----STIQRLKEERDAAVKQTQQLQHELETVRRRRNQRNSGNGLSLKLAAMVGLIGLII 213

Query: 242 GRLLR 246
           G +L+
Sbjct: 246 GFILK 213

BLAST of PI0025655 vs. ExPASy TrEMBL
Match: A0A1S3BPW9 (vesicle-associated protein 1-3-like OS=Cucumis melo OX=3656 GN=LOC103492221 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.0e-114
Identity = 230/276 (83.33%), Postives = 236/276 (85.51%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           METRLLEIHP ELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTR FTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVILVTPSISPVFSQL---------------------MNH----------QIIYEPEPAS 180
           GVILVTPSISPVF+ +                      NH           +  EPEPAS
Sbjct: 121 GVILVTPSISPVFTPVDEASNNLCKAVEEIVDQEPLNKNHVPDNKTEPRDVVEKEPEPAS 180

Query: 181 LANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN 240
           LAN+FIDSNED+AEMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQISIQETKFLQEN
Sbjct: 181 LANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQISIQETKFLQEN 240

Query: 241 FSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           FSELRK ELNRVQVGFPVLYLYMVALVCTFLGRLLR
Sbjct: 241 FSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of PI0025655 vs. ExPASy TrEMBL
Match: A0A0A0LZ53 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G573020 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.5e-114
Identity = 231/276 (83.70%), Postives = 235/276 (85.14%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           METRLLEIHP ELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDI+SSLFAKEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVILVTPSISPVFSQL---------------------MNH----------QIIYEPEPAS 180
           GVILVTPS SPVFS                        NH           +  EPEPAS
Sbjct: 121 GVILVTPS-SPVFSPADEAPNNLCEAVEEIVDQEPLNKNHVPDNNTEPRDVVEKEPEPAS 180

Query: 181 LANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN 240
            ANDFIDSNED+ EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN
Sbjct: 181 SANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN 240

Query: 241 FSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           FSELRKTELNRV+VGFPVLYLYMVALVCTFLGRLLR
Sbjct: 241 FSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of PI0025655 vs. ExPASy TrEMBL
Match: A0A6J1HAA2 (vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.7e-94
Identity = 201/294 (68.37%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M+ RLLEIHP ELKFI E NK SLCSIQLTN  +DHVAFKVK TSP KYFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LA PDMLCKD+FLIQSTVVPADTTD DI SSLFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVILVTPSISPVF-----------------------------SQLMNHQI---------- 180
           GV L++PSIS                                S +M++ +          
Sbjct: 121 GVTLISPSISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKHMVP 180

Query: 181 ----------IYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISK 240
                       EPEPASLAND ID + ++ EMIK LEELKLKLSELELKLC+AQNAISK
Sbjct: 181 GNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNAISK 240

Query: 241 LKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           LKEEKQISIQE KFLQENFSELRKTEL RVQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 LKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of PI0025655 vs. ExPASy TrEMBL
Match: A0A6J1HBX8 (vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.7e-94
Identity = 201/294 (68.37%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M+ RLLEIHP ELKFI E NK SLCSIQLTN  +DHVAFKVK TSP KYFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LA PDMLCKD+FLIQSTVVPADTTD DI SSLFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVILVTPSISPVF-----------------------------SQLMNHQI---------- 180
           GV L++PSIS                                S +M++ +          
Sbjct: 121 GVTLISPSISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKHMVP 180

Query: 181 ----------IYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISK 240
                       EPEPASLAND ID + ++ EMIK LEELKLKLSELELKLC+AQNAISK
Sbjct: 181 GNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNAISK 240

Query: 241 LKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           LKEEKQISIQE KFLQENFSELRKTEL RVQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 LKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of PI0025655 vs. ExPASy TrEMBL
Match: A0A6J1I0M1 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467905 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.9e-93
Identity = 201/294 (68.37%), Postives = 217/294 (73.81%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M+ RLLEIHP ELKFI E NK SLCSIQLTN  ++HVAFKVK TSP KYFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLEHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LA PDMLCKD+FLIQSTVVPADTTD DI SSLFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVILVTPSISPVF-----------------------------SQLMNHQI---------- 180
           GV LV+PSIS                                S +M++ +          
Sbjct: 121 GVTLVSPSISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKHMVP 180

Query: 181 ----------IYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISK 240
                       EPEPASLAND ID + ++ E+IK LEELKLKLSELELKLC+AQNAISK
Sbjct: 181 GNGTDPRDVGEEEPEPASLANDVIDGDLENLEIIKDLEELKLKLSELELKLCEAQNAISK 240

Query: 241 LKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           LKEEKQISIQE KFLQENFSELRKTEL RVQVGFPVLYLYMVALVCTFLGRLLR
Sbjct: 241 LKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRLLR 294

BLAST of PI0025655 vs. NCBI nr
Match: XP_008450729.1 (PREDICTED: vesicle-associated protein 1-3-like [Cucumis melo])

HSP 1 Score: 421.8 bits (1083), Expect = 4.1e-114
Identity = 230/276 (83.33%), Postives = 236/276 (85.51%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           METRLLEIHP ELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTR FTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVILVTPSISPVFSQL---------------------MNH----------QIIYEPEPAS 180
           GVILVTPSISPVF+ +                      NH           +  EPEPAS
Sbjct: 121 GVILVTPSISPVFTPVDEASNNLCKAVEEIVDQEPLNKNHVPDNKTEPRDVVEKEPEPAS 180

Query: 181 LANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN 240
           LAN+FIDSNED+AEMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQISIQETKFLQEN
Sbjct: 181 LANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQISIQETKFLQEN 240

Query: 241 FSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           FSELRK ELNRVQVGFPVLYLYMVALVCTFLGRLLR
Sbjct: 241 FSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of PI0025655 vs. NCBI nr
Match: XP_011659931.1 (vesicle-associated protein 2-1 [Cucumis sativus] >XP_011659932.1 vesicle-associated protein 2-1 [Cucumis sativus] >XP_031740596.1 vesicle-associated protein 2-1 [Cucumis sativus] >XP_031740621.1 vesicle-associated protein 2-1 [Cucumis sativus] >KGN66132.1 hypothetical protein Csa_007310 [Cucumis sativus])

HSP 1 Score: 419.9 bits (1078), Expect = 1.6e-113
Identity = 231/276 (83.70%), Postives = 235/276 (85.14%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           METRLLEIHP ELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDI+SSLFAKEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVILVTPSISPVFSQL---------------------MNH----------QIIYEPEPAS 180
           GVILVTPS SPVFS                        NH           +  EPEPAS
Sbjct: 121 GVILVTPS-SPVFSPADEAPNNLCEAVEEIVDQEPLNKNHVPDNNTEPRDVVEKEPEPAS 180

Query: 181 LANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN 240
            ANDFIDSNED+ EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN
Sbjct: 181 SANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLQEN 240

Query: 241 FSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           FSELRKTELNRV+VGFPVLYLYMVALVCTFLGRLLR
Sbjct: 241 FSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of PI0025655 vs. NCBI nr
Match: XP_038878362.1 (vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 380.6 bits (976), Expect = 1.0e-101
Identity = 211/288 (73.26%), Postives = 224/288 (77.78%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M+ RLLEIHP ELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKYFVRPKVGII P
Sbjct: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NST EFTVRMQ Q LAPPDMLCKDKFLIQSTVVPADTTD DI S+LFAKEGGKYIEEQKL
Sbjct: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120

Query: 121 GVILVTPSISPVFSQL-------------------------------MNHQII------- 180
           GV LV+PSISPV S +                               +N   +       
Sbjct: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180

Query: 181 -----YEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEP+ L NDFID NE++ EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           ISIQETKFL+ENFSELRKTELN+VQVGFPVLYLYMVA+VCTFLGRLLR
Sbjct: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 288

BLAST of PI0025655 vs. NCBI nr
Match: XP_022961473.1 (vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 353.2 bits (905), Expect = 1.8e-93
Identity = 201/294 (68.37%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M+ RLLEIHP ELKFI E NK SLCSIQLTN  +DHVAFKVK TSP KYFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LA PDMLCKD+FLIQSTVVPADTTD DI SSLFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVILVTPSISPVF-----------------------------SQLMNHQI---------- 180
           GV L++PSIS                                S +M++ +          
Sbjct: 121 GVTLISPSISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKHMVP 180

Query: 181 ----------IYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISK 240
                       EPEPASLAND ID + ++ EMIK LEELKLKLSELELKLC+AQNAISK
Sbjct: 181 GNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNAISK 240

Query: 241 LKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           LKEEKQISIQE KFLQENFSELRKTEL RVQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 LKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of PI0025655 vs. NCBI nr
Match: XP_022961469.1 (vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_022961470.1 vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_022961471.1 vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_022961472.1 vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 353.2 bits (905), Expect = 1.8e-93
Identity = 201/294 (68.37%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M+ RLLEIHP ELKFI E NK SLCSIQLTN  +DHVAFKVK TSP KYFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LA PDMLCKD+FLIQSTVVPADTTD DI SSLFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVILVTPSISPVF-----------------------------SQLMNHQI---------- 180
           GV L++PSIS                                S +M++ +          
Sbjct: 121 GVTLISPSISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKHMVP 180

Query: 181 ----------IYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKLCQAQNAISK 240
                       EPEPASLAND ID + ++ EMIK LEELKLKLSELELKLC+AQNAISK
Sbjct: 181 GNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNAISK 240

Query: 241 LKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGRLLR 246
           LKEEKQISIQE KFLQENFSELRKTEL RVQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 LKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of PI0025655 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 159.8 bits (403), Expect = 2.7e-39
Identity = 128/385 (33.25%), Postives = 164/385 (42.60%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M   LL+I P  L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
            ST EFTV MQ  +  PPDM+CKDKFLIQST V A+TTD DI +S+F+K  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVILVTPSISPVFSQL-----------------------------MNHQIIYEP------ 180
            V LV PS SP  S +                                +I+ EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------EPASLANDF----------------------------- 240
                                 E    AND                              
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 -------------------------------IDSNE----------------------DD 246
                                          +DS                        D+
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of PI0025655 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 159.8 bits (403), Expect = 2.7e-39
Identity = 128/385 (33.25%), Postives = 164/385 (42.60%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M   LL+I P  L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
            ST EFTV MQ  +  PPDM+CKDKFLIQST V A+TTD DI +S+F+K  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVILVTPSISPVFSQL-----------------------------MNHQIIYEP------ 180
            V LV PS SP  S +                                +I+ EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------EPASLANDF----------------------------- 240
                                 E    AND                              
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 -------------------------------IDSNE----------------------DD 246
                                          +DS                        D+
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of PI0025655 vs. TAIR 10
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.0e-34
Identity = 97/243 (39.92%), Postives = 135/243 (55.56%), Query Frame = 0

Query: 5   LLEIHPLELKFIFELNKDSLCSIQLTNKTVDH-VAFKVKITSPKKYFVRPKVGIIEPNST 64
           L+ IHP ELKF FEL K S CS+QLTNKT    VAFKVK T+P+KY VRP  G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  REFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKLGVI 124
              TV MQ Q+ AP DM CKDKFL+Q+ VV   TT  ++++ +F KE G+ IE+ KL V+
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LVTPS-ISPVFSQLMNHQIIYEPEPASLANDFIDSNEDDAEMIKHL-EELKLKLSELELK 184
            +  +  SPV            PE +   N  + S  D A     L +++     E   K
Sbjct: 126 YIPANPPSPV------------PEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSEK 185

Query: 185 LCQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLG 244
             +A + ISKL EEK  + Q+++ L+     LRK    +   G  +L + +V L+   +G
Sbjct: 186 SSEAWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIG 236

BLAST of PI0025655 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 142.9 bits (359), Expect = 3.4e-34
Identity = 91/243 (37.45%), Postives = 126/243 (51.85%), Query Frame = 0

Query: 3   TRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEPNS 62
           + LL + PL+L+F FEL K   CS+ LTNKT ++VAFKVK T+PKKY VRP  G++ P S
Sbjct: 20  SELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRS 79

Query: 63  TREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKLGV 122
           T E  V MQ Q+ AP DM CKDKFL+Q  +     T  ++   +F+KE G  +EE KL V
Sbjct: 80  TCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRV 139

Query: 123 ILVTPSISPVFSQLMNHQIIYEPEPASLANDFIDSNEDDAEMIKHLEELKLKLSELELKL 182
             V P   P         +    E  S     +  N   +E       +  K    E   
Sbjct: 140 TYVAPPRPP-------SPVHEGSEEGSSPRASVSDNGHGSEFSFERFIVDNKAGHQE-NT 199

Query: 183 CQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVALVCTFLGR 242
            +A+  I+KL EEKQ +IQ    LQ    +LR+        G P +Y+ +V L+   LG 
Sbjct: 200 SEARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGY 254

Query: 243 LLR 246
           +++
Sbjct: 260 IMK 254

BLAST of PI0025655 vs. TAIR 10
Match: AT2G45140.1 (plant VAP homolog 12 )

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-33
Identity = 89/250 (35.60%), Postives = 131/250 (52.40%), Query Frame = 0

Query: 1   METRLLEIHPLELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60
           M   LL I P++L+F FEL K   CS+ L NKT ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFAKEGGKYIEEQKL 120
            S+ E  V MQ Q+ AP D+ CKDKFL+Q  V     T  D+   +F+KE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVILVTPSISPVFSQLMNHQIIYEPEPASL-----ANDFIDSNEDDAEMIKHLEELKLKL 180
            V+ V P   P  S +           AS+     A+DF  +    A+ +          
Sbjct: 121 RVVYVAPPRPP--SPVREGSEEGSSPRASVSDNGNASDFTAAPRFSADRV---------- 180

Query: 181 SELELKLCQAQNAISKLKEEKQISIQETKFLQENFSELRKTELNRVQVGFPVLYLYMVAL 240
            + +    +A+  ++KL EEK  ++Q    LQ+   +LR+        G P +Y+ +V L
Sbjct: 181 -DAQDNSSEARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGL 237

Query: 241 VCTFLGRLLR 246
           +   LG +++
Sbjct: 241 IGLILGYIMK 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DHD73.8e-3833.25Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q84WW52.8e-3339.92Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q8VZ954.8e-3337.45Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SHC81.8e-3235.60Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q9LVU16.5e-3034.69Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3BPW92.0e-11483.33vesicle-associated protein 1-3-like OS=Cucumis melo OX=3656 GN=LOC103492221 PE=4... [more]
A0A0A0LZ537.5e-11483.70MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G573020 PE=4 SV... [more]
A0A6J1HAA28.7e-9468.37vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HBX88.7e-9468.37vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1I0M11.9e-9368.37vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_008450729.14.1e-11483.33PREDICTED: vesicle-associated protein 1-3-like [Cucumis melo][more]
XP_011659931.11.6e-11383.70vesicle-associated protein 2-1 [Cucumis sativus] >XP_011659932.1 vesicle-associa... [more]
XP_038878362.11.0e-10173.26vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida][more]
XP_022961473.11.8e-9368.37vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata][more]
XP_022961469.11.8e-9368.37vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_02296147... [more]
Match NameE-valueIdentityDescription
AT1G08820.12.7e-3933.25vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.22.7e-3933.25vamp/synaptobrevin-associated protein 27-2 [more]
AT4G00170.12.0e-3439.92Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT3G60600.13.4e-3437.45vesicle associated protein [more]
AT2G45140.11.3e-3335.60plant VAP homolog 12 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 161..209
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..244
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..109
e-value: 6.9E-26
score: 90.2
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 27.498465
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..245
e-value: 2.2E-43
score: 147.3
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..244
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 1..128
e-value: 2.0E-37
score: 129.9
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 4..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025655.1PI0025655.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane