PI0025408 (gene) Melon (PI 482460) v1

Overview
NamePI0025408
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr01: 26273396 .. 26279127 (+)
RNA-Seq ExpressionPI0025408
SyntenyPI0025408
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGTACAGGCGCGAGCCATAGCTTTTTCTTTTGCCTTCTCCGATTGAATTATGCACTGCGTTGATTTCGTTCGTTTCTCTTCCTCTTTTCATTTCCCTCCACTCCTTATATCTTATTCAAATCTTCTCTCACTTCTTTAATCAATCCAAAATTCATTTCCTTTTCTTTCCTTTTATTTGATTTCAATCCCTCTCTTTTCTTTCGATCGCCATGTCTAAAAGGTCAGTTTTCGTTTTGATTACGCTTCTTTATACGTTCTTGTTGATATTGTCACTTTTTCTTTTATGAGTTTTGATGATTGGATTACGGATTAGGATTATGGGTTCCGATAATCGCTTCTTGATATTGTTTTTTCTTCATGCTTCTTTGGTATCTTGGTTTAATTTATTAGGTATAGGCTATTTCGACGGGATGGGTTATATGATATTTGGAATTTACGTTTCATGAAAAGTGTTCTGTGGGGTTGTTGTTATTGATGCGTTTATTTTGGACTTTGGGTTGTTGTTCGTACGTGTGTTAATTTACTTTGGTGGTTTGTTTAGGGTCTTATGCAAGTATTTTGCGCATGGAGCTTGTTTAAAAGGGGAATATTGTGAGTTTTCTCATGACTGGACGGCTCCACCAAGCAATGTAAATATTACTTTCACTCCTCTGTGTTGCCATATATTATAATGGTGTTCAGACTATTGTTTCTTTCTGATGCATCGTCCATAACAGGTTTGTACTTATTATCAAAAGGGAAGGTGTGCTTTTGGCAGTGGATGCAGATATGATCATGTTAAAGTTTCTCACGCCGAATCATTTTCGAATTCTCAGCAATCTCATGGGTCTGAATCTGTTTCTGTAGCTCATTCTCAATTGTCAAGGCCACTTTCTCCTTTTTCTGGATCTACAAGCGAGCTTGCTAGTTCAGAGATACCCAGTCTTCCCTTGCGCGATGAAACTGCTTGGAGCAGAGAGTTTGTGCGTCCAGATGGTCTTGATGATTGGGGGAATGGGGAGGCAAGGGTTGTTGGTCCAGCTGATATACCTATTTGTTCTTTTGCTGCAGCTGGTGGTGATTGTCTGCGTGGTGAAAATTGTCCTCATATTCATGGGGACTTATGTCCTACATGTGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCACCTGAAGGCATGTGAGAAAAAACAAAAACATCTAGAGTCTTTGAAACATAGCCAAGACATAGAGTGCAGTGTTTGCCTGGAACGTGTTCTTTCTAAAACTGAAACCGCGGAGAGGAAATTTGGGATTCTATCAGAATGTGATCATCCATTTTGCATTTCTTGTATTAGAAACTGGCGCAATAGTTCATCTACCTCTGGGATGGATGTTAATAGTGCTTTAAGAGCCTGCCCAATTTGTAGAAAGTTGTCATACTTTGTCATTCCAAGTGTCATCTGGTATTCTTCGAAGGAAGAAAAGCAAGAGATTGTTGATAGCTACAAGTCAAGACTGCGGTAAATTTATGTTCTTTATTCCTAATTTGTGAATGCATTATGGAAACTGTTCAATTGCTTTCCATTATGTTCACGCTTTCTGCTTCCCTTTTGTTATGTTTTGTTTTGTGTGTGTGTGTGTGTGTTTTATATATATTTATCAATCTCAATTTTGTAAATTGAAGTTATTATGTATCAAATGTAATTGTATGAACTATTATTTAGAGGATGGAAACTGCATAACTGCTAAAGTAATTGTATGAGCTATTATTTGAGATGATTTCACTACTCTAATCTGTGAATTTTTATTATTTTCATGCACAGATCAATAGATTGCAAGCACTTCAACTTTGGAAATGGGAACTGTCCTTTGGATCCAGTTGCTTTTACAAGGTAAGCTTACCTCTGAAAGCAGTCACTTCAGTTTCCTTATCCTAATTGTACTCTTCCAAGCGTGATGGATGTTTTTGTAGTGGCCTTTTGTGTTGTGAGGACTTTCCAATTTCCTGAATTTTATTCTTATTCATTTTGTTTTCATTATGCCTTTGTCCAAATTGTTTTCTTTACCCTTTTTATTGATCATTGATATGCAGCCTGCTGAATGAAAATCTCAACCTGTTTGGTTTTGCTAATCTATGTACGCCCTAGGAAATCTGTTAAATAGTGCATGCTTGCCTACTGGACATGAGTTTATTAGAAGAAATTATTTTTTAGATCTGAATATGGATATCTAGTACAATGGAAGATTCTTTTACCCTAATTATTTTCCGTTGGTTTCATAAGCACCCCCATCTGCCATGGCTATCCAATCATGGCTGGTTAGATAATGCTCAATCGTACTCACATGACTTTTTTTAATTTATTTCTTTATGCTTGCTCATTAACAATTTTATATTATTTGTTCTTTGATCTATGTTTGTAAACCCTAAACAAATTATTATTAAAAAAAGCATATTTAAAAAATATGATAGTTATGTTTGAGGGATGGCTAAGAATTTAAAAGCGCTTTTAAAATGTGTAAGTTAAATAAAAATAAAGTTTTTACTTTACTTGAATTTTTGAGTAACCATCTTCTTCATTTATCTATTTAAATTTTAAGAATGTTTCAAAAATCTAAACCTACATTTGAAACGGGAAAAAATCCCTAAGAACTTTCATTTTGTTTTTAAATTTTGGTTGAGAATCTTTAAGTGTTCTTTTCCTCCAAAATTTGAAGCCCTATTTGATAACCATTTCATTTTTTCGTTTTTGTTTTTTGAAAATTAAGTTTGTCCACACCGTTTTTATCTATGGGTTTTATTATGTAATCTACTTTTTATGAGTTTTAAAAACCAAGCTATGTTTGGAAAACAAACTTGTTTTTGTACTAAGATTCAACCCTTCGTTGTTCTGATTTGAAAAAAAAATTGGCTTGAAGTCACATATATTTCTAGGAGATATGAAGACCATTATGGAGAAATGATGTGAAAAGAAGCACAATTTTCATTAACTAGAAACCAAATGGTTATCAACTAGGGCCTAAAAATCTAAATTAGGAACTGGTCAGCAATCTTGCTAAATCTTTGCAATTACTTTTGTCGTCCCTCTTTGATAATTTCTACGGTTATTCAGGTTCAAAGTGATGTGCTGACTACTTTGGCTGGTTGCCTCTACCACCTCTATGTAGTTGTAGTAATGTCTAATCAGTCTTAGTTAAATTCCTAGTACGATAAATTATATATTTTGGACACCGAGATTGTGTTGCTAGACCTGCTACAATTGGTTGATGGGATCCCTCTGATTCAAATTAATAAAAAGTTATATTCTTACAGACTAACAGGTGTTTGATTTGAAAGTGTTATCATGGGAAAATAATTGTAGTTTAGCACTGTAGCAAGGCTGGCGGGACCTCTCCTAAACTGCAATCTGTTAATCTATATATATACTTCATTTGCCAGTCATCTGCGCTCGTCTATTTTAGAAAGAACAAATGCAACTGCTGTGTCACATCTTCTTCATCCCATATGAGTGTTGCAATCTCAACAACCCTTATATTTCTTGGTGACGTGTCATAGGTGTCACATAAGCAGTGCTGTTGCTGTTCATTTTCAATCAGTTTTTGTGGTAGATGTTGAAAGATGTGATTCATAGTGGCTGTTATCTTCAGATCTATGTGCATTCTGTTTCTCATTTAATTTCATTAGAGCAGATTGGCTTTCCAATGATACAGTGTTTGTTGTGTTCCAGCATTTGGTCATTCCTGGTTCATACACATGGAAATATCACAGGCCACCTCCACGCCGACCACAATCATGGAGAACTCCAAGCAGATCTAATATTGTCGATATGGATGCCTTTTTTGAAATGTTCGAAGACTTGGAGGATTTGGATTTTAGTGACGAAGATTTAGATTTTGAATATTTAACACTTTTGGAGATGGCACTAATGCAGTTGGGTTGTGAAGCTAACTCGTCAAGTGATGAAGAGTCATAGTTTTTGGGCTACAGTTTTCTTAGGTAGTTGTGACCCGATGTATGTTGGGTGTTTGGCGAGTTCCTTTTAGTCTTTGCCCAAAAACTTTGAATATTTGCTACTCTGGCATTTTTGGCCACCTAGCAGTGCCCTTTTGTGCAGTAAGATAAGCTTGTAGACTTTTTTAGTTATCATAAATGCCATGTTATTGGGGACTGATACTTTGCAAAAGGCAACAGTCATTTGTCCTGGGGAAAATTAGTTCCCGAGTTATCAAGATGACGATGGTCTATTTATGGAAGAACCTTTTCTTTGCGTAGATTATAATATGGCTTCTTAAGTGTTATATTTTACTACTGGGAATTAGGAATATCTGCTACCAGCCATCTTCTTCATTCCTAGATTTTACGAAGAGTTCCATTTCTAACCCTCTTGCTTACCGAAACCAAGTATGCGCTCCTAATGTTGATCCTTACTGGATTGGTTGTTTATTATTGGAGTATGCCGGTAATTTGAAGTGAATGTTCGATTAAATATGTTCTATTATCTATGAAGTAGTTTTCCCATATGTGGACTTTGCACACTATGCTTGTTTTTTGAATGAAGCCTTATCTGTTTGCAACTGGGACATGTGTTGCTTCTTTACTTTCACCCATGTATTCATCTGCTTTGTAGTCAGCTGTATTAACTTCAAAATGAGAGTCAAATTTTCATGTGATTTTCTCGCTGCAGCATGCTCATCGGGATGGTCGGTTGGAAGAGATAGCTCTGCGTCATATTGGGAATGAAGATGGTCTCACTGTTATAGCTAAAAATCTTAGGTTTTGATTCGTGCATTTGCTTTATATTGACCATTTCTTGTGCATCTTTACCTTTAAATAAATTTGAAGCTATTTTCAGGTTGTCGGACTTTCTCAGTGACTTGCATATCAGGTGAAGAACCCTTCATTCAGGTATGGAACACACTAAGCACTCATCCTTTGACCCTGAGGTTGGAAGTTCAAACTTTATGCCAACTTTTGGTAGTTTTGGTAGTTTCATACTATTTTGCTATCTACTAAATATTTGGCATTTTAATATGATAATCATTTGCCTTTCTGGATGGGGTAAACTTCAATAATTAACCTTGAAATATCAATTGAAACTCAACAATTTTATTAGAAACGAAGCGGATTAATCTTCTGGCACCGTCGCGTTATCACTATATTTCAACACTTCCCTGGCTCTAAAGGGTCCATGAAATGCTAAACTCTTTGCATTTTGTATTAATCTTGAGTTGCACTTTATTTCAACTGTCATGTAAATATTTTTTTATTGTTAGTCTTGGTTAACACTTGCAACATAATCGAAGAGTTTGAAAACCCATTTCAGATGTCGAATTGCTTGGTGTGAAGTCTGATATTGAGAAATGGAAGGTGGGGGTATCGAACACGGTGCTTCAAACTTTGTGTTCTACAATATCGAAATAAAGCTAAAGACATTATGTCATAGTGAGAAATGAATGTTGGAAACCCAAGCTTGTTCTTCCAATGATATGGTGAAATAAAGATTATTACATTCACATCTCACATGTTGAATTACAGCTGAATAATATCTATGGGTTCCACAAGTTGTGTACTAAACAATTGGAAAAGGACGGAATGTATTTTGGTTTGGTATTTTAGAAGGAAGATATATGGGAATGTCAATGTTGTATTTTGTTTTATACAGAAACAAGAACAAAAAAAGTAACATCAGGGTTTGGCTTTGGATGAAACTTAGTACTCAAATTTTACAATAAAAAACTGTGATTTTTTTTCTTTTTA

mRNA sequence

GCCGTACAGGCGCGAGCCATAGCTTTTTCTTTTGCCTTCTCCGATTGAATTATGCACTGCGTTGATTTCGTTCGTTTCTCTTCCTCTTTTCATTTCCCTCCACTCCTTATATCTTATTCAAATCTTCTCTCACTTCTTTAATCAATCCAAAATTCATTTCCTTTTCTTTCCTTTTATTTGATTTCAATCCCTCTCTTTTCTTTCGATCGCCATGTCTAAAAGGGTCTTATGCAAGTATTTTGCGCATGGAGCTTGTTTAAAAGGGGAATATTGTGAGTTTTCTCATGACTGGACGGCTCCACCAAGCAATGTTTGTACTTATTATCAAAAGGGAAGGTGTGCTTTTGGCAGTGGATGCAGATATGATCATGTTAAAGTTTCTCACGCCGAATCATTTTCGAATTCTCAGCAATCTCATGGGTCTGAATCTGTTTCTGTAGCTCATTCTCAATTGTCAAGGCCACTTTCTCCTTTTTCTGGATCTACAAGCGAGCTTGCTAGTTCAGAGATACCCAGTCTTCCCTTGCGCGATGAAACTGCTTGGAGCAGAGAGTTTGTGCGTCCAGATGGTCTTGATGATTGGGGGAATGGGGAGGCAAGGGTTGTTGGTCCAGCTGATATACCTATTTGTTCTTTTGCTGCAGCTGGTGGTGATTGTCTGCGTGGTGAAAATTGTCCTCATATTCATGGGGACTTATGTCCTACATGTGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCACCTGAAGGCATGTGAGAAAAAACAAAAACATCTAGAGTCTTTGAAACATAGCCAAGACATAGAGTGCAGTGTTTGCCTGGAACGTGTTCTTTCTAAAACTGAAACCGCGGAGAGGAAATTTGGGATTCTATCAGAATGTGATCATCCATTTTGCATTTCTTGTATTAGAAACTGGCGCAATAGTTCATCTACCTCTGGGATGGATGTTAATAGTGCTTTAAGAGCCTGCCCAATTTGTAGAAAGTTGTCATACTTTGTCATTCCAAGTGTCATCTGGTATTCTTCGAAGGAAGAAAAGCAAGAGATTGTTGATAGCTACAAGTCAAGACTGCGATCAATAGATTGCAAGCACTTCAACTTTGGAAATGGGAACTGTCCTTTGGATCCAGTTGCTTTTACAAGCATGCTCATCGGGATGGTCGGTTGGAAGAGATAGCTCTGCGTCATATTGGGAATGAAGATGGTCTCACTGTTATAGCTAAAAATCTTAGGTTGTCGGACTTTCTCAGTGACTTGCATATCAGGTGAAGAACCCTTCATTCAGGTATGGAACACACTAAGCACTCATCCTTTGACCCTGAGGTTGGAAGTTCAAACTTTATGCCAACTTTTGGTAGTTTTGGTAGTTTCATACTATTTTGCTATCTACTAAATATTTGGCATTTTAATATGATAATCATTTGCCTTTCTGGATGGGGTAAACTTCAATAATTAACCTTGAAATATCAATTGAAACTCAACAATTTTATTAGAAACGAAGCGGATTAATCTTCTGGCACCGTCGCGTTATCACTATATTTCAACACTTCCCTGGCTCTAAAGGGTCCATGAAATGCTAAACTCTTTGCATTTTGTATTAATCTTGAGTTGCACTTTATTTCAACTGTCATGTAAATATTTTTTTATTGTTAGTCTTGGTTAACACTTGCAACATAATCGAAGAGTTTGAAAACCCATTTCAGATGTCGAATTGCTTGGTGTGAAGTCTGATATTGAGAAATGGAAGGTGGGGGTATCGAACACGGTGCTTCAAACTTTGTGTTCTACAATATCGAAATAAAGCTAAAGACATTATGTCATAGTGAGAAATGAATGTTGGAAACCCAAGCTTGTTCTTCCAATGATATGGTGAAATAAAGATTATTACATTCACATCTCACATGTTGAATTACAGCTGAATAATATCTATGGGTTCCACAAGTTGTGTACTAAACAATTGGAAAAGGACGGAATGTATTTTGGTTTGGTATTTTAGAAGGAAGATATATGGGAATGTCAATGTTGTATTTTGTTTTATACAGAAACAAGAACAAAAAAAGTAACATCAGGGTTTGGCTTTGGATGAAACTTAGTACTCAAATTTTACAATAAAAAACTGTGATTTTTTTTCTTTTTA

Coding sequence (CDS)

ATGTCTAAAAGGGTCTTATGCAAGTATTTTGCGCATGGAGCTTGTTTAAAAGGGGAATATTGTGAGTTTTCTCATGACTGGACGGCTCCACCAAGCAATGTTTGTACTTATTATCAAAAGGGAAGGTGTGCTTTTGGCAGTGGATGCAGATATGATCATGTTAAAGTTTCTCACGCCGAATCATTTTCGAATTCTCAGCAATCTCATGGGTCTGAATCTGTTTCTGTAGCTCATTCTCAATTGTCAAGGCCACTTTCTCCTTTTTCTGGATCTACAAGCGAGCTTGCTAGTTCAGAGATACCCAGTCTTCCCTTGCGCGATGAAACTGCTTGGAGCAGAGAGTTTGTGCGTCCAGATGGTCTTGATGATTGGGGGAATGGGGAGGCAAGGGTTGTTGGTCCAGCTGATATACCTATTTGTTCTTTTGCTGCAGCTGGTGGTGATTGTCTGCGTGGTGAAAATTGTCCTCATATTCATGGGGACTTATGTCCTACATGTGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCACCTGAAGGCATGTGAGAAAAAACAAAAACATCTAGAGTCTTTGAAACATAGCCAAGACATAGAGTGCAGTGTTTGCCTGGAACGTGTTCTTTCTAAAACTGAAACCGCGGAGAGGAAATTTGGGATTCTATCAGAATGTGATCATCCATTTTGCATTTCTTGTATTAGAAACTGGCGCAATAGTTCATCTACCTCTGGGATGGATGTTAATAGTGCTTTAAGAGCCTGCCCAATTTGTAGAAAGTTGTCATACTTTGTCATTCCAAGTGTCATCTGGTATTCTTCGAAGGAAGAAAAGCAAGAGATTGTTGATAGCTACAAGTCAAGACTGCGATCAATAGATTGCAAGCACTTCAACTTTGGAAATGGGAACTGTCCTTTGGATCCAGTTGCTTTTACAAGCATGCTCATCGGGATGGTCGGTTGGAAGAGATAG

Protein sequence

MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAESFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDGLDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDEREEHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHFNFGNGNCPLDPVAFTSMLIGMVGWKR
Homology
BLAST of PI0025408 vs. ExPASy Swiss-Prot
Match: E0X9N4 (Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=3702 GN=At3g63550 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 5.3e-112
Identity = 190/314 (60.51%), Postives = 233/314 (74.20%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKR+LCK+FAHGACLKGE+CEFSHDW  P +N+CTYYQKG C++GS CRY+HVK S   
Sbjct: 1   MSKRILCKFFAHGACLKGEHCEFSHDWKDPTNNICTYYQKGICSYGSRCRYEHVKASRPH 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
             ++S  +    S + A + L+    P     +E   S +PS  L+    W+ + +  D 
Sbjct: 61  PSASSSSAVPRPSPASASNTLACAFLP---GVAERDLSPVPSSSLK--PTWNLDSLHQDS 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           +D+        V P ++PICSFAAA GDC RG  CPHIHG +CPTCGK CLHPFR DERE
Sbjct: 121 VDEVKTSNPGTVKPEELPICSFAAA-GDCPRGNECPHIHGTICPTCGKCCLHPFRPDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EH K CEKK K LE+LK SQ+IEC VCLERVLSK   AERKFG+L+ECDH FCI+CIRNW
Sbjct: 181 EHKKVCEKKHKQLEALKLSQEIECCVCLERVLSKATPAERKFGLLTECDHAFCIACIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+SS ++GMDVNS LRACPICRKLSYFV+PSVIW+S+ EEK+EI+D+Y+ +LRSIDCKHF
Sbjct: 241 RSSSPSTGMDVNSTLRACPICRKLSYFVVPSVIWFSAPEEKKEIMDNYREKLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAF 315
           +FG+GNCP     F
Sbjct: 301 SFGDGNCPFGTSCF 308

BLAST of PI0025408 vs. ExPASy Swiss-Prot
Match: Q5ZA07 (E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=MKRN PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 5.5e-109
Identity = 197/326 (60.43%), Postives = 230/326 (70.55%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAES 61
           +KRVLCK+F HGACLKGEYCEFSHDW   P+NVCT+YQKG C++GS CRYDHVKVS   +
Sbjct: 3   TKRVLCKFFMHGACLKGEYCEFSHDWNDQPNNVCTFYQKGSCSYGSRCRYDHVKVSRNPT 62

Query: 62  FSNSQQSHGSESVSVAHSQLS--RPLSPFSGSTSELASSEIPSLPLR---DETAWSREF- 121
            +    S  +   S +   LS  RP      + S     +I    L     +  W  +F 
Sbjct: 63  VAPPPSSSTTTRASSSLQPLSFGRPHHVGYQADSSNPRQQISMDVLAHSGSKPVWRNDFQ 122

Query: 122 ---VRPDGLDDWGNG----EARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGK 181
              V  DG+ DW           + PAD+PICSF AAGG+C  GE CP +HGDLC TCGK
Sbjct: 123 HESVLEDGI-DWSISPTVQNQTTLSPADLPICSF-AAGGNCPYGEECPQMHGDLCTTCGK 182

Query: 182 QCLHPFRTDEREEHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSEC 241
            CLHP+R DEREEH K CEK  K LESLK SQ+IECSVCL+RVLSK   AERKFG+LSEC
Sbjct: 183 MCLHPYRPDEREEHTKLCEKNHKRLESLKRSQEIECSVCLDRVLSKPTAAERKFGLLSEC 242

Query: 242 DHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSY 301
           DHPFCISCIRNWRN+S TSGMDVNSALRACPICRKLSY+VIPSV+WY SKEEK EI+D+Y
Sbjct: 243 DHPFCISCIRNWRNNSPTSGMDVNSALRACPICRKLSYYVIPSVLWYFSKEEKLEIIDNY 302

Query: 302 KSRLRSIDCKHFNFGNGNCPLDPVAF 315
           K++L+SIDCK+F+FG G CP     F
Sbjct: 303 KAKLKSIDCKYFDFGTGTCPFGSSCF 326

BLAST of PI0025408 vs. ExPASy Swiss-Prot
Match: Q6IDS6 (E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-106
Identity = 187/314 (59.55%), Postives = 220/314 (70.06%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GS CRYDHV+ +   
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVRAA--- 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
             SN   S  SES+                   + + S  PS  L+ +          D 
Sbjct: 61  --SNLPLSSDSESL-------------------DRSISTTPSRHLQQQ---------GDN 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
            D   +     + P + PICSFAAA GDC RG  CPH+HGDLC TCGK+CLHPFR +ERE
Sbjct: 121 NDGDKSSNVYCIHPREYPICSFAAA-GDCPRGNQCPHMHGDLCNTCGKKCLHPFRPEERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EH K CEKKQKH+E+LK SQDIECSVCL+R+LSK    ERKFG+L+ECDHPFCI CIRNW
Sbjct: 181 EHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK++LRSIDCKHF
Sbjct: 241 RSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCKHF 280

Query: 301 NFGNGNCPLDPVAF 315
           NFGNGNCP     F
Sbjct: 301 NFGNGNCPFGASCF 280

BLAST of PI0025408 vs. ExPASy Swiss-Prot
Match: Q6GLT5 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Xenopus laevis OX=8355 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.3e-54
Identity = 120/327 (36.70%), Postives = 177/327 (54.13%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDW-TAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 61
           ++ V C+YF HG C +G  C +SHD  T+  + +C Y+Q+G CA+G  CRY+H K     
Sbjct: 35  TRHVTCRYFIHGVCKEGINCRYSHDLATSRSAMICRYFQRGCCAYGDRCRYEHNK----- 94

Query: 62  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPS-LPLRDETAWSREFVRPD 121
                Q+    ++ +     L  P    +   +ELA+SE+ S  P   +   + EFV P 
Sbjct: 95  ---PLQEDPTGDTCTAPSESLPEPSGNINSKAAELAASELASGGPRAQDWVNAVEFV-PG 154

Query: 122 GLDDWGNGEARVVG----------PAD----IPICSFAAAGGDCLRGENCPHIHGDLCPT 181
            L      EA   G          PAD      +C +AA  G+C  GENC ++HGD C  
Sbjct: 155 QLYSGRAPEAYTQGTVKPDEGREEPADPELKKQLCPYAAM-GECRYGENCVYLHGDPCDM 214

Query: 182 CGKQCLHPFRTDEREEHLKAC-EKKQKHLE---SLKHSQDIECSVCLERVLSKTETAERK 241
           CG Q LHP  T +R +H+K+C E  +K +E   +++ S+DI C +C+E V  KT  +ER+
Sbjct: 215 CGLQVLHPVDTCQRSQHIKSCIEAHEKDMELSFAVQRSKDIVCGICMEVVYEKTNPSERR 274

Query: 242 FGILSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEK 301
           FGILS C H +C+ CIR WR++        +  +++CP CR  S F+IPS  W   KEEK
Sbjct: 275 FGILSNCSHSYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFIIPSEYWVEEKEEK 334

Query: 302 QEIVDSYKSRLRSIDCKHFNFGNGNCP 309
            +++  YK  + S  C++F+ G G CP
Sbjct: 335 HKLIHKYKEAMSSKSCRYFDEGRGTCP 347

BLAST of PI0025408 vs. ExPASy Swiss-Prot
Match: Q6GLD9 (Probable E3 ubiquitin-protein ligase makorin-2 OS=Xenopus tropicalis OX=8364 GN=mkrn2 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.6e-54
Identity = 124/348 (35.63%), Postives = 180/348 (51.72%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDW-TAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 61
           +K V C+YF HG C +G  C FSHD  T+ PS VC +YQ+G+CA+G+ CRYDHVK  +  
Sbjct: 3   TKHVTCRYFLHGVCREGGRCLFSHDLATSKPSTVCRFYQRGQCAYGARCRYDHVKPCNGS 62

Query: 62  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASS-----EIPSLPLRD-------- 121
            F   Q+      ++ A  + + PL P   + + +  S     E  S+ LRD        
Sbjct: 63  VFYPPQE------MAPAPLESTPPLLPTQEAAAPVTKSAPQRREKKSVVLRDRDLCGASV 122

Query: 122 ETAWSREFVRP-----------------------DGLDDWGNGEARVVGPADIPICSFAA 181
           E A     +RP                        GLD+     A    P    +C FA 
Sbjct: 123 EKAHPDPALRPGCAADPPVSELEAKPHSYLEAICTGLDETPIPSAYPDAPQQ--LCPFAQ 182

Query: 182 AGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDEREEHLKAC----EKKQKHLESLKHSQ 241
           AGG C  GE+CP+IHG++C  CG Q LHP+  ++R  H K C    E+  +   + + S+
Sbjct: 183 AGG-CHYGESCPYIHGNVCEICGLQVLHPYDQEQRGHHEKLCMANFERDMERAFAFQASE 242

Query: 242 DIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPI 301
              CS+C+ERV  K   +ER+FGILS C H +C++CIR WR +        N  +++CP 
Sbjct: 243 GKVCSICMERVYDKQSPSERRFGILSNCHHTYCLACIRQWRCARQFE----NPVIKSCPE 302

Query: 302 CRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHFNFGNGNCP 309
           CR +S FVIPS  W   + +K E+++++K  +    CK+F+ G G CP
Sbjct: 303 CRVISEFVIPSAYWVEDQSKKFELIEAFKQGMGKKACKYFDQGRGTCP 337

BLAST of PI0025408 vs. ExPASy TrEMBL
Match: A0A0A0KBH2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G428290 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.1e-180
Identity = 304/327 (92.97%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLR+E AWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLREEAAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDW NGEARVVGPADIPICSFAAAGGDC RGENCPHIHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWVNGEARVVGPADIPICSFAAAGGDCPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMD+NSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDINSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAFTSMLI--GMVGWK 326
           NFGNGNCP     F   L+  G   WK
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWK 327

BLAST of PI0025408 vs. ExPASy TrEMBL
Match: A0A5A7UYP2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00940 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.9e-179
Identity = 300/319 (94.04%), Postives = 304/319 (95.30%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHTE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSES+SVAHSQLSRPLSPFSGSTSELASSE+PSLPLR ETAWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEARVVGPADIPICSFAAAGGDC RGENCP IHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAFTSMLI 320
           NFGNGNCP     F  +L+
Sbjct: 301 NFGNGNCPFGSSCFYKLLL 319

BLAST of PI0025408 vs. ExPASy TrEMBL
Match: A0A1S3CEC1 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499913 PE=4 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.5e-178
Identity = 299/314 (95.22%), Postives = 301/314 (95.86%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHTE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSES+SVAHSQLSRPLSPFSGSTSELASSE+PSLPLR ETAWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEARVVGPADIPICSFAAAGGDC RGENCP IHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAF 315
           NFGNGNCP     F
Sbjct: 301 NFGNGNCPFGSSCF 314

BLAST of PI0025408 vs. ExPASy TrEMBL
Match: A0A6J1GQ00 (E3 ubiquitin-protein ligase makorin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456442 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.2e-160
Identity = 274/329 (83.28%), Postives = 285/329 (86.63%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYF+HGACLKGEYCEFSHDW+APPSNVCTYYQKGRCAFGS CRYDHVKVS AE
Sbjct: 1   MSKRVLCKYFSHGACLKGEYCEFSHDWSAPPSNVCTYYQKGRCAFGSRCRYDHVKVSQAE 60

Query: 61  S--FSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRP 120
           S   SNSQQ H SESVS+AH  LSRPLSP  GSTSELA S+I   P   +  WS E   P
Sbjct: 61  SSASSNSQQPHVSESVSIAHPLLSRPLSPLPGSTSELAGSKISCPPSHVQIVWSGESEHP 120

Query: 121 DGLDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDE 180
           DGLDDW NGE R+VGPADIPICSFAAAG DC RG+NCPHIHGDLCPTCGKQCLHP RTDE
Sbjct: 121 DGLDDWRNGETRIVGPADIPICSFAAAGSDCPRGQNCPHIHGDLCPTCGKQCLHPSRTDE 180

Query: 181 REEHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIR 240
           REEH+KACEKKQKHLESLK SQDIECSVCLERVLSKTE AERKFG+LSECDHPFCISCIR
Sbjct: 181 REEHIKACEKKQKHLESLKRSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300
           NWRN+SSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK
Sbjct: 241 NWRNTSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300

Query: 301 HFNFGNGNCPLDPVAFTSMLI--GMVGWK 326
           HFNFGNGNCP     F   L+  G   WK
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYSWK 329

BLAST of PI0025408 vs. ExPASy TrEMBL
Match: A0A6J1H7L6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.6e-159
Identity = 272/329 (82.67%), Postives = 287/329 (87.23%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  S--FSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRP 120
           S   SNSQQ H SESVS+AH+ LS PLSP  GST ELA S I  LP   E+AWS E   P
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELP 120

Query: 121 DGLDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDE 180
            GLDDWG+ E R++ PAD+PICSF+AAG +C RG+NCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIR 240
           REEH+KACEKKQKHLESLKHSQ+IECSVCLERVLSKTE AERKFG+LSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKS+LRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPLDPVAFTSMLI--GMVGWK 326
           HFNFGNGNCP     F   L+  G   WK
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWK 329

BLAST of PI0025408 vs. NCBI nr
Match: XP_031744426.1 (E3 ubiquitin-protein ligase makorin isoform X1 [Cucumis sativus] >XP_031744427.1 E3 ubiquitin-protein ligase makorin isoform X1 [Cucumis sativus] >KGN45141.1 hypothetical protein Csa_015725 [Cucumis sativus])

HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 304/327 (92.97%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLR+E AWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLREEAAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDW NGEARVVGPADIPICSFAAAGGDC RGENCPHIHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWVNGEARVVGPADIPICSFAAAGGDCPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMD+NSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDINSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAFTSMLI--GMVGWK 326
           NFGNGNCP     F   L+  G   WK
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWK 327

BLAST of PI0025408 vs. NCBI nr
Match: KAA0058639.1 (E3 ubiquitin-protein ligase makorin [Cucumis melo var. makuwa] >TYK10446.1 E3 ubiquitin-protein ligase makorin [Cucumis melo var. makuwa])

HSP 1 Score: 636.0 bits (1639), Expect = 1.8e-178
Identity = 300/319 (94.04%), Postives = 304/319 (95.30%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHTE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSES+SVAHSQLSRPLSPFSGSTSELASSE+PSLPLR ETAWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEARVVGPADIPICSFAAAGGDC RGENCP IHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAFTSMLI 320
           NFGNGNCP     F  +L+
Sbjct: 301 NFGNGNCPFGSSCFYKLLL 319

BLAST of PI0025408 vs. NCBI nr
Match: XP_031744428.1 (E3 ubiquitin-protein ligase makorin isoform X2 [Cucumis sativus])

HSP 1 Score: 635.2 bits (1637), Expect = 3.1e-178
Identity = 300/314 (95.54%), Postives = 302/314 (96.18%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLR+E AWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLREEAAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDW NGEARVVGPADIPICSFAAAGGDC RGENCPHIHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWVNGEARVVGPADIPICSFAAAGGDCPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMD+NSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDINSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAF 315
           NFGNGNCP     F
Sbjct: 301 NFGNGNCPFGSSCF 314

BLAST of PI0025408 vs. NCBI nr
Match: XP_008461277.2 (PREDICTED: E3 ubiquitin-protein ligase makorin [Cucumis melo])

HSP 1 Score: 632.9 bits (1631), Expect = 1.6e-177
Identity = 299/314 (95.22%), Postives = 301/314 (95.86%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHTE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SN QQSHGSES+SVAHSQLSRPLSPFSGSTSELASSE+PSLPLR ETAWS EFVRPDG
Sbjct: 61  SCSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEARVVGPADIPICSFAAAGGDC RGENCP IHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAF 315
           NFGNGNCP     F
Sbjct: 301 NFGNGNCPFGSSCF 314

BLAST of PI0025408 vs. NCBI nr
Match: XP_038898016.1 (E3 ubiquitin-protein ligase makorin isoform X2 [Benincasa hispida])

HSP 1 Score: 612.1 bits (1577), Expect = 2.8e-171
Identity = 289/327 (88.38%), Postives = 297/327 (90.83%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGS CRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
           S SNSQQSH SESVS+AH  LS PLSPF GSTSELA SEIPSLPLR E AWS E  RPDG
Sbjct: 61  SSSNSQQSHVSESVSIAHPHLSSPLSPFPGSTSELAGSEIPSLPLRVENAWSGESERPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEAR++ PADIPICSFAAAG +C RGENCPHIHGDLCPTCGKQCLHP+RTDERE
Sbjct: 121 LDDWGNGEARIIHPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPYRTDERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EH+KACEKKQKHLESLKHSQDIECSVCLERVLSKTE AERKFG+LSECDHPFCISCIRNW
Sbjct: 181 EHMKACEKKQKHLESLKHSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPLDPVAFTSMLI--GMVGWK 326
           NFGNGNCP     F   L+  G   WK
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWK 327

BLAST of PI0025408 vs. TAIR 10
Match: AT3G08505.2 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 387.9 bits (995), Expect = 8.1e-108
Identity = 187/314 (59.55%), Postives = 220/314 (70.06%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GS CRYDHV+ +   
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVRAA--- 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
             SN   S  SES+                   + + S  PS  L+ +          D 
Sbjct: 61  --SNLPLSSDSESL-------------------DRSISTTPSRHLQQQ---------GDN 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
            D   +     + P + PICSFAAA GDC RG  CPH+HGDLC TCGK+CLHPFR +ERE
Sbjct: 121 NDGDKSSNVYCIHPREYPICSFAAA-GDCPRGNQCPHMHGDLCNTCGKKCLHPFRPEERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EH K CEKKQKH+E+LK SQDIECSVCL+R+LSK    ERKFG+L+ECDHPFCI CIRNW
Sbjct: 181 EHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK++LRSIDCKHF
Sbjct: 241 RSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCKHF 280

Query: 301 NFGNGNCPLDPVAF 315
           NFGNGNCP     F
Sbjct: 301 NFGNGNCPFGASCF 280

BLAST of PI0025408 vs. TAIR 10
Match: AT3G08505.1 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 387.9 bits (995), Expect = 8.1e-108
Identity = 187/314 (59.55%), Postives = 220/314 (70.06%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GS CRYDHV+ +   
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVRAA--- 60

Query: 61  SFSNSQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLRDETAWSREFVRPDG 120
             SN   S  SES+                   + + S  PS  L+ +          D 
Sbjct: 61  --SNLPLSSDSESL-------------------DRSISTTPSRHLQQQ---------GDN 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCLRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
            D   +     + P + PICSFAAA GDC RG  CPH+HGDLC TCGK+CLHPFR +ERE
Sbjct: 121 NDGDKSSNVYCIHPREYPICSFAAA-GDCPRGNQCPHMHGDLCNTCGKKCLHPFRPEERE 180

Query: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240
           EH K CEKKQKH+E+LK SQDIECSVCL+R+LSK    ERKFG+L+ECDHPFCI CIRNW
Sbjct: 181 EHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK++LRSIDCKHF
Sbjct: 241 RSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCKHF 280

Query: 301 NFGNGNCPLDPVAF 315
           NFGNGNCP     F
Sbjct: 301 NFGNGNCPFGASCF 280

BLAST of PI0025408 vs. TAIR 10
Match: AT5G56930.1 (CCCH-type zinc finger family protein )

HSP 1 Score: 43.1 bits (100), Expect = 4.9e-04
Identity = 19/47 (40.43%), Postives = 25/47 (53.19%), Query Frame = 0

Query: 7   CKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSGCRYDH 54
           C YFA  +C+KG+ C F HD +  P N   +  KG C  G  C + H
Sbjct: 385 CCYFATQSCMKGDDCPFDHDLSKYPCN--NFITKGFCYRGDSCLFSH 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E0X9N45.3e-11260.51Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=370... [more]
Q5ZA075.5e-10960.43E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q6IDS61.1e-10659.55E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2... [more]
Q6GLT51.3e-5436.70Probable E3 ubiquitin-protein ligase makorin-1 OS=Xenopus laevis OX=8355 GN=mkrn... [more]
Q6GLD96.6e-5435.63Probable E3 ubiquitin-protein ligase makorin-2 OS=Xenopus tropicalis OX=8364 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KBH28.1e-18092.97RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G428290 PE... [more]
A0A5A7UYP28.9e-17994.04RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CEC17.5e-17895.22RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499913 PE=4 ... [more]
A0A6J1GQ004.2e-16083.28E3 ubiquitin-protein ligase makorin-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1H7L61.6e-15982.67RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818... [more]
Match NameE-valueIdentityDescription
XP_031744426.11.7e-17992.97E3 ubiquitin-protein ligase makorin isoform X1 [Cucumis sativus] >XP_031744427.1... [more]
KAA0058639.11.8e-17894.04E3 ubiquitin-protein ligase makorin [Cucumis melo var. makuwa] >TYK10446.1 E3 ub... [more]
XP_031744428.13.1e-17895.54E3 ubiquitin-protein ligase makorin isoform X2 [Cucumis sativus][more]
XP_008461277.21.6e-17795.22PREDICTED: E3 ubiquitin-protein ligase makorin [Cucumis melo][more]
XP_038898016.12.8e-17188.38E3 ubiquitin-protein ligase makorin isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G08505.28.1e-10859.55zinc finger (CCCH-type/C3HC4-type RING finger) family protein [more]
AT3G08505.18.1e-10859.55zinc finger (CCCH-type/C3HC4-type RING finger) family protein [more]
AT5G56930.14.9e-0440.43CCCH-type zinc finger family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 204..261
e-value: 5.2E-8
score: 42.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 204..262
score: 12.430266
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 29..55
e-value: 0.079
score: 22.1
coord: 135..161
e-value: 0.43
score: 19.2
coord: 1..27
e-value: 5.7E-5
score: 32.5
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 1..28
score: 13.685178
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 134..162
score: 11.37546
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 29..56
score: 14.408138
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 204..261
e-value: 1.2E-7
score: 31.5
NoneNo IPR availableGENE3D1.20.120.1350coord: 1..32
e-value: 1.1E-6
score: 30.1
NoneNo IPR availableGENE3D3.30.1370.210coord: 33..81
e-value: 5.5E-5
score: 25.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..101
NoneNo IPR availablePANTHERPTHR11224:SF52ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 69-LIKEcoord: 1..314
NoneNo IPR availableCDDcd16521RING-HC_MKRNcoord: 204..262
e-value: 3.24197E-17
score: 72.3494
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 191..268
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 175..267
e-value: 3.0E-10
score: 41.3
IPR041367E3 ligase, CCCH-type zinc fingerPFAMPF18044zf-CCCH_4coord: 34..53
e-value: 1.3E-5
score: 24.8
IPR045072MKRN-likePANTHERPTHR11224MAKORIN-RELATEDcoord: 1..314
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 228..237
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILY90229CCCH zinc fingercoord: 3..26
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILY90229CCCH zinc fingercoord: 32..53

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025408.2PI0025408.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding