PI0025306 (gene) Melon (PI 482460) v1

Overview
NamePI0025306
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionNEDD8 ultimate buster 1
Locationchr05: 1618798 .. 1624062 (-)
RNA-Seq ExpressionPI0025306
SyntenyPI0025306
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTACAATTTGACCTTTTTATTTTTTAAAAGTGGAAATCGAATATTTGATTTTGAAGTTAATAGAATAACCATTAGACGATTGAATTGAATGTATTTTCAATATTCATGTGTAGAAATGTTACACGTAATAAAAAGAATTAAATGAGCTAAATTTAAAATTGACGGGAAGTAACATTGAACAAATGTAGGACTAAATCTGATTTTTTCTTTTAATCAAAGATAAAGTTCTGATCTTTAAAAGGTGGATAGGCTATGGCCAAGGCCCAAGCCGAACACGCAAACTCGCGTCAACAGACTTGAAAGCAGAGAAATTTGATCCAATTTGTACCGAAATTCCAAGACTTGACCAACAAGGAAACCTCCTCGTACCTTTTAAATCCTCCTTTCATTAATTCCAATCATACCCACCTCGATTTTCTCCATCTTCTCTCATCTGAAGGATCAAAAGACCACTCAGTAACTTTCCCACTCTCCCTTTTTAGAGTCCCAATTTCTATTTTGGTTCAGTTTTCATGGCCAAATTGAAGATTTCAGGTCCATGGACTGGTGTGTTGGATGTAGAACTCGATAGTTGGACTGTCCCCATGTTGAGGGAGGAAATTGCAAGGCGATCGAACTGTGGTGTTGAGTCGATCAATTTAATTTCTGGTGGTAAAATCTTGAAAGATGGCGATGGAAGCGAGAAGCTGGTCCATTTGGGTCTTAAGAACAACTCTAGGATTCTTGCTAGACGGGTTTCTACTGAAGAGGGGAAGGCACTCAAGAATGAATTGATGGCTGAGGAAGAACGCTCTACCAGGCTTGCTCGAGTCAAGTAATTCTTTGACTTTTTATTTCTGTAATTATGTTTGTATCTTCTCTTATAACGATGCTTGTTAGGATTATGTGCAATGTCATTGCTGTGTTCATGAGTTTCAACTGGGTTTGCTTTGTTTGCTTGTGTTTCAATAGATTTTGCTATTTTGGGAAGCTTGGTTTTGTAGAATGATGATTCCATGAAGAAATTGGTAAAAAAATTTTAAAGTTTTTGGGGTTAAATGGGCTTGGTTTTCACTGTCAAGTTTTTTTTCTTGAAGTAATAGTGGAAATACGATTTTAAAAGCACAATGAAAGTTGGATTTTGGAAAGATGCTTAAGTTCTTCTATGGTTTAGGCTTTAACAGTTGATTATGGAAGCAAAGCATCTAGTTCAGGAATGAAAAGCAAAATTTATTTGTAAGATGCAATGTGGCATAATGAATCAAATGACAGTCAACTTTTGGAGAGTGTTTTTCCCCATGGGAGGGTCATGAGTAATTTTGAAGATTTTAAAGACATTTCCTTTTATATTGCTGATGTTGATGTTGAACGTTTGTTTTACGTCGTTTAGTATCTATCTGATTTTCTGGCATCTTCTGAACTTCCAGGGCAGCTGCCACTGCTCTAGCTGGGAGGCATGCTAATGGTTTCCTCCCAGTTGAAGATTTCAATATAGAACTTGAGGATCAAAGTGGTCAGAAAGTTAATTTTGGGTCTGAAACTGACCAAAAGTAAGTAATAAATCATTCTCAACATGTGAATTCATGTTTCCTCATGTCAAATGTTATAAGTATATTTGTTGTACATTCAACAGGGCAATCATGATGGGCCTTATGCTTCATGCAAACGCAAAGCAGCTGATAAGAAAGGGAAATTACAAAGATGCATTAGAAGTACTCGTCATGGGAGAGGTCTGAGCTTGGTTGATCATGTCAATTAGTCTTCAATATTAGCTTCTAAATTTTAGCTGTTTTCCCCCTTTTAAGAAATAGACAAGATTGAAATGATTTTAGATAAACTTGAAGATTAAACCATTTATCGAGGAAGGTAAACATCTTCCTCAAAAGGAAAGGATAAAATAAATATATCACTCCAGTATGGTTGATCTGCTCTACAGGTGACAACTGAGTTTGAAGTGCTGTGCTTGATGATCTTTATTGGCCAATGGTTCCAAATTCTAATATCTATGACTGTTCAATTCTACTTTTGCTTTCTTTACCTTTAAATTGAAGGTGGTAAAAGAAAGCACTGTTTATGGTTTGATTATCCCAAGTTGTTGTGCCAGATACTGATATCAGCATACTTTTATTTACTATGAGAGAATACTAATCAATGTGGATTATGTTCTTGTAGGAGGCTTTCACTCTTTGCAATCCAAAACTTATTGAGGTGAATGACTTCTCCTTTATCATGAGTTCCTACTTCCTCCCCACCACCACCAAGACTAATTGCTAAAGTTATTTATAATTGTAAAAGAAAGAGAAATCTAAGTTTCTCAAACTATTAAACTATTCATTTATAACATTGCAACTGAAGTTAGTATCAGATCTTTTAAATACTATTAAGGCTGGTTTGGGTTATGATTGTGATATGATTTGAGGCGTCAGATTGAGACTACGTGAAAATACAAAAAATTTAATGATTTTCCTTATCTTCTATATGTGACCAATGGCGGTGTTTCCATCACAGTTGTATCAATGTAGTTCTTATTTTTTTAAAAAATAAAATAAAATTGATGTGGTTTTTAGCGTTGTTTGCTCAGAATATTTGTATAGCTTGCTGACTGAGTATGAGCCTTCTTCTTTGTCAGTTTGTTGACAACGTCTCAATACTTCAAATAGACATGGTTTGGTGTTATTTCATGCTAAAAGACATCAAGTGGCTCTCAGATGCCGGATTACGCCTTGCAAAGGCTAGAGAAGGAATCGAACGTGCGCATGGGAAGGACTCTTCCCGTGTTCGAATCCTTCAAGCTGGTTGCCATCCTGAGCTTGCCTTGTAAGTCCGCTGCATGTTATTCTCAACAGTTTGAGGGATGGTTTTCAGATACAAGCTAATTACGTTTTGTCATAATGTTAGACATTTGAGACTGGAGCTGCTGGAGGGGGTGGCTGCATATCATAGCTGTAAGTTTGATAAGGCTCGGGAAGCAATCAACTCAGCACGAGCAAAGTATTTTCAGGTTAGCTTATCATTTTACAGAATTGGGTGGATCAAGCTTGCGCATGTTTATAGATTGAATGGTGTACTGGCGGAGATATCAGGCCTAATATATGTTGAGCAATTTGGTTTGATAAAAATGTCACTACAGTTGTTTATAGCTAGAGTTCCATGTTGAGCTTTAAAGATGGTCTCTTTGTTTTTCTATGCAACCTAATACATTCTACCTTGAAATTTCAGCTACAAGTGTCAGATGAAGCATTATCGCATGTTATGGATATGGGATTTAAAGAAAAAGAAGCAAAGAGAGCACTGCGCATGTGCCATCAAGATGTATGTAAGTCTGTTGATTTTCTCTTTGAGGAAAGGACAAAGAGAGAGAGAAAACGGGAAGAGGATATTCGACGCCAGGAAGAAATCATGTATGTGTTCTGAGCAAAACTAGCTTTTATTTTCTTCCAGTAGTTTATGAGCTGTCTATAATATTAATAACCCGAATAAAATTATCAGGGAGCAAAAACTATATGGAATGACGCCCTCCAAGAAAGCTGTCAATCTTCAGATATTGGAAAAACTGGCATCTATAGGGTAAGTAGACTAACCAATGTTGTTTTTTTCTTCCAAAATGTCAGAAGAAAAACATATCAATGAATGATAACTTTGTTTTTGTATGTGGCAACTAATGTGATTGTTGCGGAATTACTTCTCATCAAGACTTTTCATGAATGGTTGCATCAGAAACATAGTGTAATATGGATCCTGATGTATCTTTTATAGAAGTATATTTGGATATTGGAGTTGGAAGCTGTTCAAATTTGGACTAAAATTTAGTCTTATAAGGCTCCTTAACTCGTCATTTGGTACCTATAATGGGATCCCGATCTAACTTTCTTAGAAGTCTGTTAGGAGTTGAAAGTGGACTGAGTTCAGACTAAACTCAACTGTCTTATAAGGGAATGGCCAAGGTTGTCTCAAGCAAGTATATTTTAGAATAATTCAAATCTTGGTCTATCCATGATATGTGACAAATATCGGGCTTTTCTGGTGGGAACCTAACCTGGAAAACTTCAGAACTCTTGATATTCGTTGATATGTTTTACAACTCACTTTGGAAGTTTAATAATACTTTCAGCTAATCACCTTTTAAATTGTCTCATTTTATGTTTTGTGTTGATGAGCTCTTCGATTAAAATTGTTTGGCAAGCCACAACTTCGTGATTTGTGACCACTTTTATCAATTAAGTTGCAATTTATATGTGCAAGGTTTGAGAATTGTTGTAGTTATTATTCAAGCTAAAACTAGCCTGGTTTTTTAGGTATGAGAAGGAATTAGCTGCCGAAGCCCTGAGAAGAAATGAGAATGACATACAGAAAGCATTGGATGATTTGACAAGCCCAGAATCTAACTCTGTTATACAGGTGTCTTGGATTTTAATCTTTATCCAGTGTGAGTTGGTTTTTTCATTTAAACTTTTACCTTGCTAAATTCGTGCCATTCACAATTCTGCAGAATGCTATCGAGTCGAGGAAAAGGAAACGACAACGTAAAAGGGCAGATGATGCAGTTGATAAGCTGGTTCGCATGGGTTTTGATAAAACAAGAGGTATGTATGATCAAACCAATATAGGGCGCTTATTATAAATCCAGAAACTCTTTATAGGACTCATGTATCTAAGTACAACAACAAACTTGTTGATCTTATATTATTAATATTAAGAAACTTTGATCAAACTAGTAATGCTATAAAATCGTTACTGTGTGCAGCTGAGGCAGCTTTTGAGGCTGGTGGCTCCTTAGAGCAAGCACTAATTATATTATCAGAACCTGGGATGAACGCTACGGTCGATGGGCAACCTGACTCGGTCAATGCAGCCTTGGCTTCTGAGGGTGCAAATTCCTCCCTTTCAAACATTGCAGAAAACCAAGAAATGTTGGACACCTTGGATAACGAGGGAGGGCCTTCTACTGGTGAAGAAGACCGAGACTTGGAGATGGAAGATGAACTCGCTAGAGAACTCAATGGCGCTACTGCCGTATCTGATTACGACATGGATGTAACAAAAGAAGGAGAAGCCATAACAGAGTACTTGGGTTTGTTGGACTCAATGATGAATTCTGAGAATGCTTAAGTAACATTTTAACAGGGTATTTATGCTGTAAATAATATTGCTTTTGAATTCTGAATTTGGTTAGCTTCCTGTAAGTAAAAGAGGTACTGTTTAGAGATAAAACTATCCTTGGAACTGTTGTTGCTACAACAAGAAGAATAAGAGTTAGTAACGTTTGAGCTTACTTTGATGAAAA

mRNA sequence

AAGTACAATTTGACCTTTTTATTTTTTAAAAGTGGAAATCGAATATTTGATTTTGAAGTTAATAGAATAACCATTAGACGATTGAATTGAATGTATTTTCAATATTCATGTGTAGAAATGTTACACGTAATAAAAAGAATTAAATGAGCTAAATTTAAAATTGACGGGAAGTAACATTGAACAAATGTAGGACTAAATCTGATTTTTTCTTTTAATCAAAGATAAAGTTCTGATCTTTAAAAGGTGGATAGGCTATGGCCAAGGCCCAAGCCGAACACGCAAACTCGCGTCAACAGACTTGAAAGCAGAGAAATTTGATCCAATTTGTACCGAAATTCCAAGACTTGACCAACAAGGAAACCTCCTCGTACCTTTTAAATCCTCCTTTCATTAATTCCAATCATACCCACCTCGATTTTCTCCATCTTCTCTCATCTGAAGGATCAAAAGACCACTCAGTAACTTTCCCACTCTCCCTTTTTAGAGTCCCAATTTCTATTTTGGTTCAGTTTTCATGGCCAAATTGAAGATTTCAGGTCCATGGACTGGTGTGTTGGATGTAGAACTCGATAGTTGGACTGTCCCCATGTTGAGGGAGGAAATTGCAAGGCGATCGAACTGTGGTGTTGAGTCGATCAATTTAATTTCTGGTGGTAAAATCTTGAAAGATGGCGATGGAAGCGAGAAGCTGGTCCATTTGGGTCTTAAGAACAACTCTAGGATTCTTGCTAGACGGGTTTCTACTGAAGAGGGGAAGGCACTCAAGAATGAATTGATGGCTGAGGAAGAACGCTCTACCAGGCTTGCTCGAGTCAAGGCAGCTGCCACTGCTCTAGCTGGGAGGCATGCTAATGGTTTCCTCCCAGTTGAAGATTTCAATATAGAACTTGAGGATCAAAGTGGTCAGAAAGTTAATTTTGGGTCTGAAACTGACCAAAAGGCAATCATGATGGGCCTTATGCTTCATGCAAACGCAAAGCAGCTGATAAGAAAGGGAAATTACAAAGATGCATTAGAAGTACTCGTCATGGGAGAGGAGGCTTTCACTCTTTGCAATCCAAAACTTATTGAGTTTGTTGACAACGTCTCAATACTTCAAATAGACATGGTTTGGTGTTATTTCATGCTAAAAGACATCAAGTGGCTCTCAGATGCCGGATTACGCCTTGCAAAGGCTAGAGAAGGAATCGAACGTGCGCATGGGAAGGACTCTTCCCGTGTTCGAATCCTTCAAGCTGGTTGCCATCCTGAGCTTGCCTTACATTTGAGACTGGAGCTGCTGGAGGGGGTGGCTGCATATCATAGCTGTAAGTTTGATAAGGCTCGGGAAGCAATCAACTCAGCACGAGCAAAGTATTTTCAGCTACAAGTGTCAGATGAAGCATTATCGCATGTTATGGATATGGGATTTAAAGAAAAAGAAGCAAAGAGAGCACTGCGCATGTGCCATCAAGATGTATGTAAGTCTGTTGATTTTCTCTTTGAGGAAAGGACAAAGAGAGAGAGAAAACGGGAAGAGGATATTCGACGCCAGGAAGAAATCATGGAGCAAAAACTATATGGAATGACGCCCTCCAAGAAAGCTGTCAATCTTCAGATATTGGAAAAACTGGCATCTATAGGGTATGAGAAGGAATTAGCTGCCGAAGCCCTGAGAAGAAATGAGAATGACATACAGAAAGCATTGGATGATTTGACAAGCCCAGAATCTAACTCTGTTATACAGAATGCTATCGAGTCGAGGAAAAGGAAACGACAACGTAAAAGGGCAGATGATGCAGTTGATAAGCTGGTTCGCATGGGTTTTGATAAAACAAGAGCTGAGGCAGCTTTTGAGGCTGGTGGCTCCTTAGAGCAAGCACTAATTATATTATCAGAACCTGGGATGAACGCTACGGTCGATGGGCAACCTGACTCGGTCAATGCAGCCTTGGCTTCTGAGGGTGCAAATTCCTCCCTTTCAAACATTGCAGAAAACCAAGAAATGTTGGACACCTTGGATAACGAGGGAGGGCCTTCTACTGGTGAAGAAGACCGAGACTTGGAGATGGAAGATGAACTCGCTAGAGAACTCAATGGCGCTACTGCCGTATCTGATTACGACATGGATGTAACAAAAGAAGGAGAAGCCATAACAGAGTACTTGGGTTTGTTGGACTCAATGATGAATTCTGAGAATGCTTAAGTAACATTTTAACAGGGTATTTATGCTGTAAATAATATTGCTTTTGAATTCTGAATTTGGTTAGCTTCCTGTAAGTAAAAGAGGTACTGTTTAGAGATAAAACTATCCTTGGAACTGTTGTTGCTACAACAAGAAGAATAAGAGTTAGTAACGTTTGAGCTTACTTTGATGAAAA

Coding sequence (CDS)

ATGGCCAAATTGAAGATTTCAGGTCCATGGACTGGTGTGTTGGATGTAGAACTCGATAGTTGGACTGTCCCCATGTTGAGGGAGGAAATTGCAAGGCGATCGAACTGTGGTGTTGAGTCGATCAATTTAATTTCTGGTGGTAAAATCTTGAAAGATGGCGATGGAAGCGAGAAGCTGGTCCATTTGGGTCTTAAGAACAACTCTAGGATTCTTGCTAGACGGGTTTCTACTGAAGAGGGGAAGGCACTCAAGAATGAATTGATGGCTGAGGAAGAACGCTCTACCAGGCTTGCTCGAGTCAAGGCAGCTGCCACTGCTCTAGCTGGGAGGCATGCTAATGGTTTCCTCCCAGTTGAAGATTTCAATATAGAACTTGAGGATCAAAGTGGTCAGAAAGTTAATTTTGGGTCTGAAACTGACCAAAAGGCAATCATGATGGGCCTTATGCTTCATGCAAACGCAAAGCAGCTGATAAGAAAGGGAAATTACAAAGATGCATTAGAAGTACTCGTCATGGGAGAGGAGGCTTTCACTCTTTGCAATCCAAAACTTATTGAGTTTGTTGACAACGTCTCAATACTTCAAATAGACATGGTTTGGTGTTATTTCATGCTAAAAGACATCAAGTGGCTCTCAGATGCCGGATTACGCCTTGCAAAGGCTAGAGAAGGAATCGAACGTGCGCATGGGAAGGACTCTTCCCGTGTTCGAATCCTTCAAGCTGGTTGCCATCCTGAGCTTGCCTTACATTTGAGACTGGAGCTGCTGGAGGGGGTGGCTGCATATCATAGCTGTAAGTTTGATAAGGCTCGGGAAGCAATCAACTCAGCACGAGCAAAGTATTTTCAGCTACAAGTGTCAGATGAAGCATTATCGCATGTTATGGATATGGGATTTAAAGAAAAAGAAGCAAAGAGAGCACTGCGCATGTGCCATCAAGATGTATGTAAGTCTGTTGATTTTCTCTTTGAGGAAAGGACAAAGAGAGAGAGAAAACGGGAAGAGGATATTCGACGCCAGGAAGAAATCATGGAGCAAAAACTATATGGAATGACGCCCTCCAAGAAAGCTGTCAATCTTCAGATATTGGAAAAACTGGCATCTATAGGGTATGAGAAGGAATTAGCTGCCGAAGCCCTGAGAAGAAATGAGAATGACATACAGAAAGCATTGGATGATTTGACAAGCCCAGAATCTAACTCTGTTATACAGAATGCTATCGAGTCGAGGAAAAGGAAACGACAACGTAAAAGGGCAGATGATGCAGTTGATAAGCTGGTTCGCATGGGTTTTGATAAAACAAGAGCTGAGGCAGCTTTTGAGGCTGGTGGCTCCTTAGAGCAAGCACTAATTATATTATCAGAACCTGGGATGAACGCTACGGTCGATGGGCAACCTGACTCGGTCAATGCAGCCTTGGCTTCTGAGGGTGCAAATTCCTCCCTTTCAAACATTGCAGAAAACCAAGAAATGTTGGACACCTTGGATAACGAGGGAGGGCCTTCTACTGGTGAAGAAGACCGAGACTTGGAGATGGAAGATGAACTCGCTAGAGAACTCAATGGCGCTACTGCCGTATCTGATTACGACATGGATGTAACAAAAGAAGGAGAAGCCATAACAGAGTACTTGGGTTTGTTGGACTCAATGATGAATTCTGAGAATGCTTAA

Protein sequence

MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLVHLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVEDFNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLCNPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQAGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFKEKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNLQILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRADDAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANSSLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAITEYLGLLDSMMNSENA
Homology
BLAST of PI0025306 vs. ExPASy Swiss-Prot
Match: P54729 (NEDD8 ultimate buster 1 OS=Mus musculus OX=10090 GN=Nub1 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 9.9e-34
Identity = 144/541 (26.62%), Postives = 254/541 (46.95%), Query Frame = 0

Query: 26  LREEIARRSNCGVESINLISGGKILKDGDGSEKLVHLGLKNNSRILARRVSTEEGKALKN 85
           LR +IA         I ++   K L+ G   + L   G+ +N + +   +   E    KN
Sbjct: 113 LRCQIAETFGFQENYIKIVINKKQLQLG---KSLEEQGVTHNVKAMVLELKQSEEDVRKN 172

Query: 86  ELMAEEE------RSTRLARVKAAATALAGRHANGFLPVEDFNIELEDQSGQKVNFGSET 145
             + EEE      +  R+ R K     LA R      P     +++ +Q+G+ +      
Sbjct: 173 LQLEEEEQNEAELKERRIQRTKRGLEILAERAEMVVDPETMPYLDIANQTGRSLRI-PPA 232

Query: 146 DQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLCNPKLIEFVDNVSILQIDMV 205
           ++KA+M+ +  H   +  +++  Y  AL  L+  +  F  C  +L++ VDN ++LQ+D+V
Sbjct: 233 ERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADRYFCECK-ELLDTVDNYAVLQLDIV 292

Query: 206 WCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQAGCHPELALHLRLELLEGV 265
           WCYF L+ ++ L DA  +L  A++  +  +G++  R+  ++  C  E  L LRL LL+G+
Sbjct: 293 WCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGI 352

Query: 266 AAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFKEKEAKRALRMCHQDVCKSV 325
             YHS   ++ARE +N AR  + +L +    + +++ +GF  +EA+  LR C  +V  + 
Sbjct: 353 QNYHSGNGEEAREYLNKARQLFKELYIDPSKVHNLLQLGFTAQEARLGLRACDGNVDHAA 412

Query: 326 DFLFEERTKRERKREE--DIRRQEEIMEQKLYGMTPSKKAVNLQILEKLASIGYEKELAA 385
                  T    +REE   IR++E+            K+   L+ +  L  +GY  + A 
Sbjct: 413 -------THISNRREELAQIRKEEK-----------EKRRRRLENVNTLRGMGYSTQAAK 472

Query: 386 EALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRADDAVDKLVRMGFDKTRAE 445
           +AL +   ++  AL  L S      +Q+A +     RQ   + +++++LV MGFD   AE
Sbjct: 473 QALHQARGNLDDALKVLLSNPHMWWLQDA-DPENNSRQASPSQESINQLVYMGFDTVVAE 532

Query: 446 AAFEA-GGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANSSLSNIAENQEMLDTLD 505
           AA    GG+++ A   L+  G +   D         L   G +SS +      +      
Sbjct: 533 AALRVFGGNVQLAAQTLAHHGGSLPPD---------LQFSGEDSSPTPSTSPSD------ 592

Query: 506 NEGGPSTGEEDRDLEME--DELARELNGATAVSDYDMDVTKEGE--AITEYLGLLDSMMN 554
              G S+   D D+E E  +E+  ++       DY +D T E E   I EYL  ++S+ +
Sbjct: 593 -SAGTSSASTDEDMETEAVNEILEDI--PEHEEDY-LDSTLEDEEVIIAEYLSYVESISS 610

BLAST of PI0025306 vs. ExPASy Swiss-Prot
Match: Q9Y5A7 (NEDD8 ultimate buster 1 OS=Homo sapiens OX=9606 GN=NUB1 PE=1 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 1.7e-33
Identity = 131/501 (26.15%), Postives = 227/501 (45.31%), Query Frame = 0

Query: 26  LREEIARRSNCGVESINLISGGKILKDGDGSEKLVHLGLKNNSRILARRVSTEEGKALKN 85
           LR +IA         I ++   K L+ G   + L   G+ +N + +   +   E  A KN
Sbjct: 112 LRSKIAETFGLQENYIKIVINKKQLQLG---KTLEEQGVAHNVKAMVLELKQSEEDARKN 171

Query: 86  ELMAEEE------RSTRLARVKAAATALAGRHANGFL-PVEDFNIELEDQSGQKVNFGSE 145
             + EEE      +  ++ R K     LA R A   + P     +++ +Q+G+ +     
Sbjct: 172 FQLEEEEQNEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRI-PP 231

Query: 146 TDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLCNPKLIEFVDNVSILQIDM 205
           +++KA+M+ +  H   +  +++  Y  AL  L+  ++ F  C  +L++ VDN ++LQ+D+
Sbjct: 232 SERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDI 291

Query: 206 VWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQAGCHPELALHLRLELLEG 265
           VWCYF L+ ++ L DA  +L  A++  +  +G++  R+  ++  C  E  L LRL LL+G
Sbjct: 292 VWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQG 351

Query: 266 VAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFKEKEAKRALRMCHQDVCKS 325
           +  YHS    +A E +N AR  + +L +    + +++ +GF  +EA+  LR C  +V  +
Sbjct: 352 IRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHA 411

Query: 326 VDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNLQILEKLASIGYEKELAAE 385
              +   R +  + R+E+                  KK   L+ +  L  +GY    A +
Sbjct: 412 ATHITNRREELAQIRKEE----------------KEKKRRRLENIRFLKGMGYSTHAAQQ 471

Query: 386 ALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRADDAVDKLVRMGFDKTRAEA 445
            L     ++ +AL  L S      + N        RQ   + + +D+LV MGFD   AEA
Sbjct: 472 VLHAASGNLDEALKILLSNPQMWWL-NDSNPETDNRQESPSQENIDRLVYMGFDALVAEA 531

Query: 446 AFEA---------------GGSLEQALIILSEPGMNATVDGQPDSVNAALAS-------E 498
           A                  GGSL   L +  E  ++       DS   + AS       E
Sbjct: 532 ALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPATSPSDSAGTSSASTDEDMETE 589

BLAST of PI0025306 vs. ExPASy TrEMBL
Match: A0A0A0LI64 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008690 PE=4 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 8.3e-294
Identity = 530/556 (95.32%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELD+WTV MLREEIARRSNCGVESINLISGG+ILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
           HLGLKNNSRILARRV+ EEGKALKNELMAEEERS+RLARVKAAATALA RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+REAINSARAKYFQLQVSD+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMC+QDVCK+VDFLFEERT+RERKREED+RRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           QILEKLASIGYEKELAAEALRRNENDIQKALDDL SPESNSVIQNA+ESRKRKRQRKRAD
Sbjct: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
           DAVDKLV MGFDKTRAEAAFEAGGSLEQA+IILS+PG N+TVDGQPDSVNAAL SEGA+S
Sbjct: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLD+EGGPS GEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of PI0025306 vs. ExPASy TrEMBL
Match: A0A1S3C5V6 (NEDD8 ultimate buster 1 OS=Cucumis melo OX=3656 GN=LOC103497022 PE=4 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.1e-293
Identity = 534/556 (96.04%), Postives = 540/556 (97.12%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELD+WTV MLREEIARRSNC VESINLISGG+ILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVRMLREEIARRSNCSVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
           HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALA RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRMLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMCHQDVCK+VDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCHQDVCKAVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           QILEKL SIGYEKELAAEALRRNENDIQKALDDL SPESNSVIQNAIESRKRKRQRKR D
Sbjct: 361 QILEKLTSIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAIESRKRKRQRKRVD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
            AVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPG N  V GQPDSVNA LASEGA+S
Sbjct: 421 GAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGTNTAVHGQPDSVNAVLASEGASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLD+EGG S GEEDRD  MEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGSSIGEEDRDSVMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of PI0025306 vs. ExPASy TrEMBL
Match: A0A6J1FCD2 (NEDD8 ultimate buster 1 OS=Cucurbita moschata OX=3662 GN=LOC111444333 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.7e-259
Identity = 474/556 (85.25%), Postives = 510/556 (91.73%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELD+WTV MLREEIARRSNCGVESINLI GG+ILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
            LG+KNNSRILARRVSTEEGK+L  ELMAEEERS RLARVKAAATALA RHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDKAREAINSA+ KY QLQV DEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMCHQDVCK+VDFLFEERTKR++KREEDI RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNEND+QKALDDL +PE+NSVIQNA+ESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQA+ +LS P   +TVDGQPDSV    ASE A+S
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSASEYASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  DN  GPST EE+RD EME ELAREL+GA+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYL LLDS+MNSE A
Sbjct: 541 TEYLALLDSLMNSEKA 556

BLAST of PI0025306 vs. ExPASy TrEMBL
Match: A0A6J1HMA3 (NEDD8 ultimate buster 1 OS=Cucurbita maxima OX=3661 GN=LOC111465491 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.1e-257
Identity = 472/556 (84.89%), Postives = 507/556 (91.19%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELD+WTV MLREEIARRSNCGVESINLI GG+ILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
            LG+KNNSRILA RVSTEEGK+L  ELMAEEERS RLARVKAAATALA RHANGFLPVED
Sbjct: 61  DLGVKNNSRILAMRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPK+IEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDKAREAINSA+ KY QLQV DEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMCHQDVCK+VDFLFEERTKRE+KREEDI RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNENDIQKALDDL +PE+NS IQNA+ESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDIQKALDDLINPETNSFIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQA+ +LS P   +TVDGQPDSV  A ASE A+S
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTASASEDASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  DN  GPST EE+RD EME ELAREL+ A+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDRASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYL LLDS+MNS  A
Sbjct: 541 TEYLALLDSLMNSAKA 556

BLAST of PI0025306 vs. ExPASy TrEMBL
Match: A0A6J1CMT5 (NEDD8 ultimate buster 1 OS=Momordica charantia OX=3673 GN=LOC111013048 PE=4 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 2.1e-249
Identity = 456/557 (81.87%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPW GVL+VELD+WTVPMLREE+ARRSNCG+ESINLI GG++LKDGDGSEKL+
Sbjct: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
           +LG+KNNSRILARRVSTEEGK+LK+ELMAEEERSTRLARVKAAATALA RHANGFLPVED
Sbjct: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKV+ GSETDQ+AIMMGLMLHANAKQLI+KGNYK+ALEVLVMGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDKA++AINSA+AKYFQLQV DEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           E+EAK ALRMCHQDV  +VDFL  ER KRE+KREEDI+R++EI EQK YGMTPSKKAV+L
Sbjct: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLAS-IGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRA 420
           Q L++L S IGYE+ELAAEALRRNEND QKALDDLT+PE+NS+IQN IESRKRKRQR+ A
Sbjct: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420

Query: 421 DDAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGAN 480
           D  VD LVRMGFD+T+A AA +A GSLEQAL +LS P  N TVDGQPDS  AA+A EGAN
Sbjct: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480

Query: 481 SSLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEA 540
           SSLSNI   ++MLD  D+  GPST EE+RD EME +LA ELNGATAVSDYDMDVTKEGEA
Sbjct: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540

Query: 541 ITEYLGLLDSMMNSENA 557
           ITEYL  LDS+ N E A
Sbjct: 541 ITEYLVFLDSVENLEKA 557

BLAST of PI0025306 vs. NCBI nr
Match: XP_004139279.1 (NEDD8 ultimate buster 1 [Cucumis sativus] >KGN60729.1 hypothetical protein Csa_019386 [Cucumis sativus])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.7e-293
Identity = 530/556 (95.32%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELD+WTV MLREEIARRSNCGVESINLISGG+ILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
           HLGLKNNSRILARRV+ EEGKALKNELMAEEERS+RLARVKAAATALA RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+REAINSARAKYFQLQVSD+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMC+QDVCK+VDFLFEERT+RERKREED+RRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           QILEKLASIGYEKELAAEALRRNENDIQKALDDL SPESNSVIQNA+ESRKRKRQRKRAD
Sbjct: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
           DAVDKLV MGFDKTRAEAAFEAGGSLEQA+IILS+PG N+TVDGQPDSVNAAL SEGA+S
Sbjct: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLD+EGGPS GEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of PI0025306 vs. NCBI nr
Match: XP_008457303.1 (PREDICTED: NEDD8 ultimate buster 1 [Cucumis melo])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.5e-292
Identity = 534/556 (96.04%), Postives = 540/556 (97.12%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELD+WTV MLREEIARRSNC VESINLISGG+ILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVRMLREEIARRSNCSVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
           HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALA RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRMLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMCHQDVCK+VDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCHQDVCKAVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           QILEKL SIGYEKELAAEALRRNENDIQKALDDL SPESNSVIQNAIESRKRKRQRKR D
Sbjct: 361 QILEKLTSIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAIESRKRKRQRKRVD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
            AVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPG N  V GQPDSVNA LASEGA+S
Sbjct: 421 GAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGTNTAVHGQPDSVNAVLASEGASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLD+EGG S GEEDRD  MEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGSSIGEEDRDSVMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of PI0025306 vs. NCBI nr
Match: XP_038890750.1 (NEDD8 ultimate buster 1 [Benincasa hispida])

HSP 1 Score: 965.7 bits (2495), Expect = 1.7e-277
Identity = 509/556 (91.55%), Postives = 533/556 (95.86%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELD WTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKL+
Sbjct: 1   MAKLKISGPWTGVLDVELDIWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLI 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
           HLGLKNNSRILARRVSTEEGKALK+ELMAEEERSTRLARVKAAATALA RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKDELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIK+LSDAG+RLA+AREGIER+HGKDSSRVRILQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKFLSDAGIRLARAREGIERSHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           E+EAKRALRMCHQDVCK+VDFLFEER KRER+REEDIRRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EREAKRALRMCHQDVCKAVDFLFEERAKRERRREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           QIL++LASIGYEKELAAEALRRNEND+QKALDDL SPESNSVIQNAIESRKRKRQ K AD
Sbjct: 361 QILKELASIGYEKELAAEALRRNENDLQKALDDLISPESNSVIQNAIESRKRKRQHKTAD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
           DAVD LVRMGFD+T+A AAFEAGGSLEQALIILSE   N TV+GQPDSV AALASEG +S
Sbjct: 421 DAVDNLVRMGFDRTKAGAAFEAGGSLEQALIILSELETNTTVNGQPDSVTAALASEGTSS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQ MLDT D+  GP  GEEDRDLEMEDELAREL+GA+AVSDYD+DVTKEGEAI
Sbjct: 481 SLSNIAENQ-MLDTFDDGEGPE-GEEDRDLEMEDELARELDGASAVSDYDLDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYL LL++ MNSE A
Sbjct: 541 TEYLNLLETTMNSEKA 554

BLAST of PI0025306 vs. NCBI nr
Match: XP_023537732.1 (NEDD8 ultimate buster 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.5 bits (2331), Expect = 1.8e-258
Identity = 474/556 (85.25%), Postives = 509/556 (91.55%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELD+WTV MLREEIARRSNCGVESINLI GG+ILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
            LG+KNNSRILARRVSTEEGK+L  ELMAEEERS RLARVKAAATALA RHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPK+IEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDKAREAINSA+ KY QLQV DEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMCHQDVCK+VDFLFEERTKRE+KREEDI RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNENDIQKALDDL +PE+NSVIQNA+ESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDIQKALDDLINPETNSVIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQA+ +LS P   +TVDGQPDSV     SE A+S
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSGSEDASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  DN  GPST EE+RD EME ELAREL+GA+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYL LLDS+MNSE A
Sbjct: 541 TEYLALLDSLMNSEKA 556

BLAST of PI0025306 vs. NCBI nr
Match: XP_022938166.1 (NEDD8 ultimate buster 1 [Cucurbita moschata])

HSP 1 Score: 902.5 bits (2331), Expect = 1.8e-258
Identity = 474/556 (85.25%), Postives = 510/556 (91.73%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELD+WTV MLREEIARRSNCGVESINLI GG+ILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPVED 120
            LG+KNNSRILARRVSTEEGK+L  ELMAEEERS RLARVKAAATALA RHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNIELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDKAREAINSA+ KY QLQV DEALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMCHQDVCK+VDFLFEERTKR++KREEDI RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNEND+QKALDDL +PE+NSVIQNA+ESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGMNATVDGQPDSVNAALASEGANS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQA+ +LS P   +TVDGQPDSV    ASE A+S
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSASEYASS 480

Query: 481 SLSNIAENQEMLDTLDNEGGPSTGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  DN  GPST EE+RD EME ELAREL+GA+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 557
           TEYL LLDS+MNSE A
Sbjct: 541 TEYLALLDSLMNSEKA 556

BLAST of PI0025306 vs. TAIR 10
Match: AT2G12550.1 (ubiquitin-associated (UBA)/TS-N domain-containing protein )

HSP 1 Score: 575.1 bits (1481), Expect = 6.1e-164
Identity = 318/565 (56.28%), Postives = 414/565 (73.27%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDSWTVPMLREEIARRSNCGVESINLISGGKILK-DGDGSEKL 60
           MAKLK++G W G+L+VEL++WT+ MLR+E+A+RS    ESI LI  GKILK DGD +  L
Sbjct: 1   MAKLKVAGTWGGILEVELENWTLNMLRDEVAKRSGLDPESIKLIFAGKILKDDGDATLTL 60

Query: 61  VHLGLKNNSRILA-RRVSTEEGKALKNELMAEEERSTRLARVKAAATALAGRHANGFLPV 120
             LG+K NS+IL+ R+ + EEGK+    +MAEEER+ RL+RVKAAATAL+ RHA+G LP+
Sbjct: 61  SQLGIKENSKILSCRQAAPEEGKS----IMAEEERARRLSRVKAAATALSKRHADGSLPI 120

Query: 121 EDFNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFT 180
           +D+NIELEDQ G KV FG+ETDQ A MMGLMLH  AK LI    Y DA+EVL M EE+F 
Sbjct: 121 QDYNIELEDQGGHKVQFGTETDQSAAMMGLMLHTKAKSLIEIEMYSDAVEVLAMAEESFL 180

Query: 181 LCNPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRI 240
           LC+PK++E VDN+ +++ID+VWCYF+L+DIK LSDAG+RL +AR+G+ERAHGKD SRVR+
Sbjct: 181 LCDPKILELVDNLPMMEIDIVWCYFLLRDIKCLSDAGVRLVRARKGLERAHGKDLSRVRL 240

Query: 241 LQAGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMG 300
           LQAG  PE+A++LRLELLEGV AYH+ + DKA  A+ SA AK+ QLQ+ DE LS VM MG
Sbjct: 241 LQAGQSPEMAIYLRLELLEGVVAYHTGQNDKALNALKSAHAKFLQLQIPDETLSLVMGMG 300

Query: 301 FKEKEAKRALRMCHQDVCKSVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAV 360
           F+EK+AKRALR+ +QD+  SVDFL EER K+ +KRE+D++RQ EI+EQK YG+TP KKAV
Sbjct: 301 FQEKDAKRALRLNNQDIASSVDFLIEERAKKAQKREQDLQRQSEILEQKKYGVTPMKKAV 360

Query: 361 NLQILEKLASIGYEKELAAEALRRNENDIQKALDDLTSPESNSVIQNAIESRKRKRQRKR 420
           ++Q+LE+L SIGY +ELAAE+LRRNENDIQKALD LT P+ NS IQ  IESRKRKRQ + 
Sbjct: 361 DMQMLERLVSIGYARELAAESLRRNENDIQKALDILTDPKVNSTIQAYIESRKRKRQEQL 420

Query: 421 ADDAVDKLVRMGFDKTRAEAAFEAGGSLEQAL-----IILSEPGMNAT--VDGQPDSVNA 480
              +V +LV MGF++ +A +A EAGGS E  +       ++ PG   T  ++    + N 
Sbjct: 421 VGISVAELVSMGFERGKATSALEAGGSREDTIQRLLSASVANPGTTTTSVINATSSTNNV 480

Query: 481 ALASEGANSSLSNIAE-NQEMLDTLDNEGGPSTG--EEDRDLEMEDELARELNGATAVSD 540
              S G        +E   E  D + N    S+   EE+RD E+EDE+A E+    A+S 
Sbjct: 481 GAESSGFGGGAEQDSEMKDETTDDIANRASTSSDGMEEERDSEIEDEIADEIARVDALSA 540

Query: 541 YDMDVTKEGEAITEYLGLLDSMMNS 554
           YD+++ KE EAI EYL +LD+   S
Sbjct: 541 YDINLDKEIEAINEYLAMLDASQES 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P547299.9e-3426.62NEDD8 ultimate buster 1 OS=Mus musculus OX=10090 GN=Nub1 PE=1 SV=2[more]
Q9Y5A71.7e-3326.15NEDD8 ultimate buster 1 OS=Homo sapiens OX=9606 GN=NUB1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LI648.3e-29495.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008690 PE=4 SV=1[more]
A0A1S3C5V67.1e-29396.04NEDD8 ultimate buster 1 OS=Cucumis melo OX=3656 GN=LOC103497022 PE=4 SV=1[more]
A0A6J1FCD28.7e-25985.25NEDD8 ultimate buster 1 OS=Cucurbita moschata OX=3662 GN=LOC111444333 PE=4 SV=1[more]
A0A6J1HMA38.1e-25784.89NEDD8 ultimate buster 1 OS=Cucurbita maxima OX=3661 GN=LOC111465491 PE=4 SV=1[more]
A0A6J1CMT52.1e-24981.87NEDD8 ultimate buster 1 OS=Momordica charantia OX=3673 GN=LOC111013048 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004139279.11.7e-29395.32NEDD8 ultimate buster 1 [Cucumis sativus] >KGN60729.1 hypothetical protein Csa_0... [more]
XP_008457303.11.5e-29296.04PREDICTED: NEDD8 ultimate buster 1 [Cucumis melo][more]
XP_038890750.11.7e-27791.55NEDD8 ultimate buster 1 [Benincasa hispida][more]
XP_023537732.11.8e-25885.25NEDD8 ultimate buster 1 [Cucurbita pepo subsp. pepo][more]
XP_022938166.11.8e-25885.25NEDD8 ultimate buster 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G12550.16.1e-16456.28ubiquitin-associated (UBA)/TS-N domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015940Ubiquitin-associated domainSMARTSM00165uba_6coord: 419..455
e-value: 8.3
score: 7.3
coord: 359..396
e-value: 0.034
score: 23.3
coord: 287..324
e-value: 4.2E-4
score: 29.6
IPR015940Ubiquitin-associated domainPROSITEPS50030UBAcoord: 357..397
score: 10.595029
IPR015940Ubiquitin-associated domainPROSITEPS50030UBAcoord: 285..325
score: 12.209535
NoneNo IPR availableGENE3D3.10.20.90coord: 8..83
e-value: 1.8E-5
score: 26.3
NoneNo IPR availableGENE3D1.10.8.10coord: 361..395
e-value: 1.3E-5
score: 27.0
coord: 287..326
e-value: 1.3E-9
score: 39.9
NoneNo IPR availableGENE3D1.10.8.10coord: 396..457
e-value: 3.5E-5
score: 25.7
NoneNo IPR availableCDDcd14270UBAcoord: 363..392
e-value: 5.61873E-4
score: 35.4045
IPR039749NEDD8 ultimate buster 1PANTHERPTHR12948NEDD8 ULTIMATE BUSTER-1 BS4 PROTEINcoord: 20..549
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 22..70
score: 9.046888
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 12..71
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 350..396
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 405..456
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 279..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025306.1PI0025306.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:2000058 regulation of ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0031593 polyubiquitin modification-dependent protein binding
molecular_function GO:0005515 protein binding