PI0025140 (gene) Melon (PI 482460) v1

Overview


Name	PI0025140
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	cytochrome b5
Location	chr11: 2425935 .. 2427290 (+)
RNA-Seq Expression	PI0025140
Synteny	PI0025140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAACTCTGCTCAAGGGAAGGATTAGAAAAAAAGTTTGATAGGAAATGGATTCAAATTCAAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAGGATTGTTGGCTTGTAATCTCTGGATATGTATGTTCTTTATTTCCCTTTTCTTCTTTTTAACCTCTCTCTAATTACACTAATTCATTGCTTAATTAATATTTCTATTACATCATCATAAAGATTTGGATTATGAGAAACAGAGAAGTCTCCGTATTATCATCTCGTTCTCTCACGTTCATGATGTCAAAATATCAATTCTAACGCATCCCCTTGTATCTAAGTTTTAGAAGAGTGTGAGTGTTGTTGTCTTATAATAATTACTCCTTGTCTGGAGTGTTTTTGGAGGGTTGTGAATTATGATGTTGTGTCCAGTTTTGTTTGTTTTGCTGTTTTGTATCTGTATTACCCTAGCTAAATGAATTGTTTTTAGGTGTACGATGTAACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTGGGTGAGATGCTACACACCCATTTCTAATCATACAAATCCATCTCTAATTAATTACTTTTTCTAATGTTTTATATATGCACATTAACATGGTTTAATTATTATATATATTTTACTCTTTATACTAGTTTATATATAATTTTAGAAATCTTTAAATTAGTCTTCTGTCGTTTCTTTGAAATTAAGATTTATGTAACTTTTCAAACATATTTTTGCTTAAATAATCATGGAATGGAATCACGACTGTCAAACTAACATTCTGCTTAAGTAGTCACATACCACTAAAAAGCTTAAACTTCGATTTTCTTGTAATCACTGGTTGTAGTAGGAAAATTAATTGAACTTCTAACCTTGAGATTACTATTACAATTTTTTTTTTTTTTTTTTGTATTATTTGAATTATACTTTATCATTCTAACAACTAATGTTGAAATGTTTTGAGGATTGACGTAATAGATTTGGATATATGCAGAAAAAGATGCAACGTTTGATTTTAAGAGTGTTGGACACAGCGAATTAGCTCAAGAGAAGATGAAAAAGTATCGAATAGGGAAGATCGACATGTCGACTCTCCCTGGGAAACAAAAATATGTGGAAAGTACACCATATCAGCCAACTCCAAGTCTATTTTCCTTCTCACCCATCCATGTTATACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAAAATTCATTATTTTACATTTCTATTTTACTTTCGCACAAGGATTATAGCCCACAGGCTAAACAATAATAATTTGTAGCTTTAACTTCATTATTGAATGGTTCAAC

mRNA sequence

GAAACTCTGCTCAAGGGAAGGATTAGAAAAAAAGTTTGATAGGAAATGGATTCAAATTCAAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAGGATTGTTGGCTTGTAATCTCTGGATATGTGTACGATGTAACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCAACGTTTGATTTTAAGAGTGTTGGACACAGCGAATTAGCTCAAGAGAAGATGAAAAAGTATCGAATAGGGAAGATCGACATGTCGACTCTCCCTGGGAAACAAAAATATGTGGAAAGTACACCATATCAGCCAACTCCAAGTCTATTTTCCTTCTCACCCATCCATGTTATACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAAAATTCATTATTTTACATTTCTATTTTACTTTCGCACAAGGATTATAGCCCACAGGCTAAACAATAATAATTTGTAGCTTTAACTTCATTATTGAATGGTTCAAC

Coding sequence (CDS)

ATGGATTCAAATTCAAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAGGATTGTTGGCTTGTAATCTCTGGATATGTGTACGATGTAACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCAACGTTTGATTTTAAGAGTGTTGGACACAGCGAATTAGCTCAAGAGAAGATGAAAAAGTATCGAATAGGGAAGATCGACATGTCGACTCTCCCTGGGAAACAAAAATATGTGGAAAGTACACCATATCAGCCAACTCCAAGTCTATTTTCCTTCTCACCCATCCATGTTATACTCCCTATTTTGCTCTTAGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAGGCCTAA

Protein sequence

MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLALAFAFPYFKLKA

Homology

[Close]

BLAST of PI0025140 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 3.8e-29
Identity = 69/136 (50.74%), Postives = 90/136 (66.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN  KDCWL+ISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQP------TPSLFSFSPIHVILP 120
            VGHS+ A++ M KY IG+ID S++P  + YV   P QP      TP  F    +  ++P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of PI0025140 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-28
Identity = 68/136 (50.00%), Postives = 88/136 (64.71%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S  KV  F+EV++HN  KDCWL+ISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQP------TPSLFSFSPIHVILP 120
            VGHS+ A++ M+KY IG+ID ST+P  + YV   P QP      TP  F    +  ++P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA     +  K
Sbjct: 121 ILILGLALVVRQYTKK 133

[Close]

BLAST of PI0025140 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 8.0e-27
Identity = 62/132 (46.97%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +KV++  EV++HN+ KDCWLVISG VYDVT FL DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQP-----TPSLFSFSPIHVILPI 120
            VGHS  A+  + +Y +G ID +T+P K KY  + P QP       S F    +  ++P+
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

Query: 121 LLLALAFAFPYF 128
           ++L +AF   ++
Sbjct: 121 IILGVAFGIRFY 130

[Close]

BLAST of PI0025140 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 3.0e-26
Identity = 62/128 (48.44%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 2   DSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKS 61
           + N KVY+ +EVAKHN + DCWL+I G VY+V+ FL DHPGGD++LL +  KDAT DF+ 
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 62  VGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQP------TPSLFSFSPIHVILPI 121
           VGHS  A+  M +Y +G ID ST+P + KYV   P QP      TP  F    +  ++P+
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYV--PPKQPHYNQDKTPE-FIIKILQFLVPL 122

Query: 122 LLLALAFA 124
            +L LA A
Sbjct: 123 AILGLAVA 127

[Close]

BLAST of PI0025140 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.0e-26
Identity = 62/137 (45.26%), Postives = 86/137 (62.77%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  + KV++  EV++H+  KDCW+VI G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVEST-----PYQPTPSLFSFSPIHVILPI 120
            VGHS  A+  + +Y +G ID +T+P K K+V  T       Q   S F    +  ++P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KLKA 132
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of PI0025140 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.2e-65
Identity = 126/131 (96.18%), Postives = 128/131 (97.71%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNHQKDCWL+ISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSELAQEKMK YRIGKIDMSTLP KQ YVESTPYQPTPSLFSFSPIHV+LPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of PI0025140 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-62
Identity = 121/131 (92.37%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWL+ISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSELA EKMK Y+IGKIDMSTLP KQKYVES PYQ TPSLFSFSPIH++LPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of PI0025140 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.5e-56
Identity = 104/131 (79.39%), Postives = 121/131 (92.37%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK+Y+F+EVAKHNH+KDCWL+ISGYVYDVTS+LPDHPGG ELL+L VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSE A+E MKKY+IGKID  TLP +QKYVE+TPY+PTPSL SFSP+HV++P+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of PI0025140 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.5e-55
Identity = 104/131 (79.39%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHNH+KDCWL+ISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSE A+E+MKKY+IGKID  TLP +QK+VE+TP++PTPSL SFSP+HV++P+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of PI0025140 vs. ExPASy TrEMBL
Match: M5X125 (Cytochrome b5 heme-binding domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.2e-33
Identity = 75/133 (56.39%), Postives = 97/133 (72.93%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+S+VY FDEVAKHNH+KDCW++ISG VYDVT FL DHPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKY---VESTPYQPTPSLFSFSPIHVILPILL 120
            VGHS+ A+E+M+K+ +GK+D ST+P +  Y    ++TP     S F    +  +LP+L+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTTPPSGQSSGFVVKLLQFLLPLLI 120

Query: 121 LALAFAFPYFKLK 131
           L  AFA  Y+  K
Sbjct: 121 LGAAFALQYYGKK 133

[Close]

BLAST of PI0025140 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 258.8 bits (660), Expect = 2.5e-65
Identity = 126/131 (96.18%), Postives = 128/131 (97.71%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNHQKDCWL+ISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSELAQEKMK YRIGKIDMSTLP KQ YVESTPYQPTPSLFSFSPIHV+LPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of PI0025140 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 247.7 bits (631), Expect = 5.7e-62
Identity = 121/131 (92.37%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWL+ISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSELA EKMK Y+IGKIDMSTLP KQKYVES PYQ TPSLFSFSPIH++LPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of PI0025140 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 244.6 bits (623), Expect = 4.8e-61
Identity = 115/131 (87.79%), Postives = 128/131 (97.71%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDS+SK+YSFDEV+KHNH +DCWL+ISGYVYDVT+FLPDHPGG+ELLLLAV +DATFDFK
Sbjct: 3   MDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFDFK 62

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSELAQEKMKKYRIGKIDMSTLP KQ+YVE+TPYQPTPSLFSFSPIH+++PILLLAL
Sbjct: 63  SVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLLAL 122

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 123 AFAFPYFKLKA 133

[Close]

BLAST of PI0025140 vs. NCBI nr
Match: XP_023000205.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 226.5 bits (576), Expect = 1.4e-55
Identity = 104/131 (79.39%), Postives = 121/131 (92.37%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK+Y+F+EVAKHNH+KDCWL+ISGYVYDVTS+LPDHPGG ELL+L VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSE A+E MKKY+IGKID  TLP +QKYVE+TPY+PTPSL SFSP+HV++P+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of PI0025140 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 225.3 bits (573), Expect = 3.0e-55
Identity = 104/131 (79.39%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHNH+KDCWL+ISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQPTPSLFSFSPIHVILPILLLAL 120
           SVGHSE A+E+MKKY+IGKID  TLP +QK+VE+TP++PTPSL SFSP+HV++P+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of PI0025140 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 129.0 bits (323), Expect = 2.7e-30
Identity = 69/136 (50.74%), Postives = 90/136 (66.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN  KDCWL+ISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQP------TPSLFSFSPIHVILP 120
            VGHS+ A++ M KY IG+ID S++P  + YV   P QP      TP  F    +  ++P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of PI0025140 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 119.0 bits (297), Expect = 2.8e-27
Identity = 62/137 (45.26%), Postives = 86/137 (62.77%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  + KV++  EV++H+  KDCW+VI G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVEST-----PYQPTPSLFSFSPIHVILPI 120
            VGHS  A+  + +Y +G ID +T+P K K+V  T       Q   S F    +  ++P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KLKA 132
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of PI0025140 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 116.7 bits (291), Expect = 1.4e-26
Identity = 58/126 (46.03%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HN   DCW+VI+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQKYVESTPYQP-----TPSLFSFSPIHVILPI 120
            VGHSE A+E M++Y +G+ID +T+P K KY  + P QP       S F    +  ++P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLLALA 122
            +L LA
Sbjct: 121 AILGLA 124

[Close]

BLAST of PI0025140 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 112.1 bits (279), Expect = 3.5e-25
Identity = 55/131 (41.98%), Postives = 84/131 (64.12%), Query Frame = 0


Query: 5   SKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGH 64
           + + SF +VAKH  + DCW++I G VYD+++F+ +HPGGD +LL    KDA+ DF+ V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 65  SELAQEKMKKYRIGKIDMSTLPGKQKYV-----ESTPYQPTPSLFSFSPIHVILPILLLA 124
           S+ A+E MKKY IG +D ST+P  Q+Y+     EST  + T        +  ++P+L+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 125 LAFAFPYFKLK 131
           +AFA  ++  K
Sbjct: 122 VAFALRFYNNK 132

[Close]

BLAST of PI0025140 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 110.2 bits (274), Expect = 1.3e-24
Identity = 53/126 (42.06%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLVISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M + +K+YS +E A HN Q DCW+VI G VYDV+S++ +HPGGD++LL    KDAT DF+
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  SVGHSELAQEKMKKYRIGKIDMSTLPGKQK---YVESTPYQPTPSLFSFSPIHVILPILL 120
             GHS+ A+E M+KY IG++D S+LP   +   Y +  P      LF  +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LALAFA 124
           + ++ A
Sbjct: 121 ITISVA 126

The following BLAST results are available for this feature:

BLAST of PI0025140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	3.8e-29	50.74	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	1.5e-28	50.00	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	8.0e-27	46.97	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	3.0e-26	48.44	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
Q9ZWT2	4.0e-26	45.26	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]

BLAST of PI0025140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CAE5	1.2e-65	96.18	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1	[more]
A0A0A0KA20	2.7e-62	92.37	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1KHQ2	6.5e-56	79.39	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1	[more]
A0A6J1HJ10	1.5e-55	79.39	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1	[more]
M5X125	1.2e-33	56.39	Cytochrome b5 heme-binding domain-containing protein OS=Prunus persica OX=3760 G...	[more]

BLAST of PI0025140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459741.1	2.5e-65	96.18	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_004140502.1	5.7e-62	92.37	cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc...	[more]
XP_038875836.1	4.8e-61	87.79	cytochrome b5-like [Benincasa hispida]	[more]
XP_023000205.1	1.4e-55	79.39	cytochrome b5-like [Cucurbita maxima]	[more]
XP_022964486.1	3.0e-55	79.39	cytochrome b5-like [Cucurbita moschata]	[more]

BLAST of PI0025140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	2.7e-30	50.74	cytochrome B5 isoform E	[more]
AT5G48810.1	2.8e-27	45.26	cytochrome B5 isoform D	[more]
AT2G32720.1	1.4e-26	46.03	cytochrome B5 isoform B	[more]
AT2G46650.1	3.5e-25	41.98	cytochrome B5 isoform C	[more]
AT1G26340.1	1.3e-24	42.06	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 64.74 coord: 68..80 score: 36.13 coord: 55..62 score: 35.8 coord: 40..54 score: 44.55
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 1.1E-26 score: 104.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 1.6E-22 score: 79.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 27.0399
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..84 e-value: 2.1E-31 score: 109.8
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..85
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..129
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..129
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0025140.1	PI0025140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding