PI0024609 (gene) Melon (PI 482460) v1

Overview
NamePI0024609
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr05: 14061012 .. 14062918 (-)
RNA-Seq ExpressionPI0024609
SyntenyPI0024609
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATGGACCACCTCAAGAAAGATCTCAAACAACTCCTTTATAGAATCATCGAGGAAGAAGATTTCGATTCCTCCGCCGCCGACGAAGCTATTCACATATTGACTGCTTTGAAATTCTGTTTAATGCGCGCTCCTTCCTCCCCGGAACCCACCGCCACTTCTGGCACTAACACTCCGATTGTCCCCGACAAGTTCCGGTGTCCGATTTCCGGCGACCTCATGAAAGACCCTGTTCTCTTGATTACCGGTCAGGTTTGTGATTTTTTTTCCTCATATCATTTGTTTTTCTTTTCAATTTTGTTGGGCCTTCATAATATTTTTGTGTTTCAAGATTGTGTTGCTACTACCATGAATTTGTCTCTGTTGCCTCTGTTTTCTTTTTAATTTTCTGTAGGCTCTGTTCTTTTGTGTTCATCATTACGATTTTTTAGTCTTTTAAAAAAAAGATGATATTCATTTCTCCTATGGCTCTCTAGTGTCTAATTTTAATGATTGAGGTTTCTTTGTTCCATAAAGTTAACTTTGCTTTTTTTTTTTTTTTTTTTTTGCAACTATATGTAAATTCGCATTGCCACATATATTACTTCTTTTATGTCTAAAAACATGTTTAATTATAATGAATCTATGTTCTCTAACCCAATGTCTAATTTCAGTGATTGGGTTTTGTGTTAATTTTATGAGGGAAGTTGTTACAAATATGTATGAAGGGTTGGATATACTAATCTACTGGTTCTTTAAGAAAAATTCTCTGTGAATTGTTTATGTGTATGTGTTGCAGTTTGAGTTTGTGTTTAATGTTCTTGAAACATGGTTTGGAATTCTAGACATATGATCGGTTCTTCATCGAAAAGTGGTTCCATGAAGGCCACAATACATGTCCTCAAACCAAGGAAGTCCTTACCGACGTGACTCTTACCCCGAATCGTCTTCTTCGGTCGATGATCTCGCAATGGTGCTTAGATAACAGATTTGAACTGCCAAGGCTTACTTATGAGGAAGAAGTCGACAATGTAACTGAGTCCCATTTAGATGCATTGCTTAACAGGTTGATGTCTTCATCCCTAATAGTTAAAAAAGAAGCTGCAAAGGAGTTGAGAGAGACAACAAGATGGAGCCATGAATTTCGAGCTCTTTTCGCAAAGCTCCCGGCGTCCATTGAGAGATTATTGCATCCTCTGATCTCAATTGGCAAAGTTGATCTCCATCCTGATCTTCGAGAGGATTTGATTACCACTATTCTCAATATCTCAATTTTTGATGATAACAAGAAACATGTAGCGGAGAACCCGTTGGTTCTCCCTTTGTTAATTGAATCCTTACAGTACGGAAATATTGAGGCGACAGCGAATGCAGTTGCAGCTATTTACTCATTATCTCTCAATGAGACAAATAAGATAACGATGGGAAAAGTAGGAATTTTCAAACATCTGATTTCAGTTTTAGACTATGCCCATCCTGTAGTGATAAGAGATGCTGGTTCAGCAATCTACAACCTCTGTACCGTCGTCGAAAACAGAGAGAAGGCAGTTGGCAGTGGAGTAGTTGCCTCCATTTTTCGAAACATCGGTCGAAGCTTATTATTGGTCGATAAGTTGATTTCGATACTTGCACTTCTTTCCACCGATATGAAGGCTATTGACGAGATGTGCAAGTTCGATGCAGTGCCTTGTATGCTGAGGATCATAAGGGAAACTGAGTCCCAACGCATCAAAGAAAATTGTGCATCGATACTCTTTGCAATTTGCACAACTGATCGAAGCCAATTAAGGAAGATCCAGGAGGATGAAAATAAAAATGAGACGATCTTGGAACTTTCAAAGAATGGTAATTCAAGGGCAAGGAGAAAGGCTACCGGCATACTCGATAGGATGCATAGAGCTGCTTCAAGAACTCACACTTCCTAA

mRNA sequence

ATGGCCATGGACCACCTCAAGAAAGATCTCAAACAACTCCTTTATAGAATCATCGAGGAAGAAGATTTCGATTCCTCCGCCGCCGACGAAGCTATTCACATATTGACTGCTTTGAAATTCTGTTTAATGCGCGCTCCTTCCTCCCCGGAACCCACCGCCACTTCTGGCACTAACACTCCGATTGTCCCCGACAAGTTCCGGTGTCCGATTTCCGGCGACCTCATGAAAGACCCTGTTCTCTTGATTACCGGTCAGACATATGATCGGTTCTTCATCGAAAAGTGGTTCCATGAAGGCCACAATACATGTCCTCAAACCAAGGAAGTCCTTACCGACGTGACTCTTACCCCGAATCGTCTTCTTCGGTCGATGATCTCGCAATGGTGCTTAGATAACAGATTTGAACTGCCAAGGCTTACTTATGAGGAAGAAGTCGACAATGTAACTGAGTCCCATTTAGATGCATTGCTTAACAGGTTGATGTCTTCATCCCTAATAGTTAAAAAAGAAGCTGCAAAGGAGTTGAGAGAGACAACAAGATGGAGCCATGAATTTCGAGCTCTTTTCGCAAAGCTCCCGGCGTCCATTGAGAGATTATTGCATCCTCTGATCTCAATTGGCAAAGTTGATCTCCATCCTGATCTTCGAGAGGATTTGATTACCACTATTCTCAATATCTCAATTTTTGATGATAACAAGAAACATGTAGCGGAGAACCCGTTGGTTCTCCCTTTGTTAATTGAATCCTTACAGTACGGAAATATTGAGGCGACAGCGAATGCAGTTGCAGCTATTTACTCATTATCTCTCAATGAGACAAATAAGATAACGATGGGAAAAGTAGGAATTTTCAAACATCTGATTTCAGTTTTAGACTATGCCCATCCTGTAGTGATAAGAGATGCTGGTTCAGCAATCTACAACCTCTGTACCGTCGTCGAAAACAGAGAGAAGGCAGTTGGCAGTGGAGTAGTTGCCTCCATTTTTCGAAACATCGGTCGAAGCTTATTATTGGTCGATAAGTTGATTTCGATACTTGCACTTCTTTCCACCGATATGAAGGCTATTGACGAGATGTGCAAGTTCGATGCAGTGCCTTGTATGCTGAGGATCATAAGGGAAACTGAGTCCCAACGCATCAAAGAAAATTGTGCATCGATACTCTTTGCAATTTGCACAACTGATCGAAGCCAATTAAGGAAGATCCAGGAGGATGAAAATAAAAATGAGACGATCTTGGAACTTTCAAAGAATGGTAATTCAAGGGCAAGGAGAAAGGCTACCGGCATACTCGATAGGATGCATAGAGCTGCTTCAAGAACTCACACTTCCTAA

Coding sequence (CDS)

ATGGCCATGGACCACCTCAAGAAAGATCTCAAACAACTCCTTTATAGAATCATCGAGGAAGAAGATTTCGATTCCTCCGCCGCCGACGAAGCTATTCACATATTGACTGCTTTGAAATTCTGTTTAATGCGCGCTCCTTCCTCCCCGGAACCCACCGCCACTTCTGGCACTAACACTCCGATTGTCCCCGACAAGTTCCGGTGTCCGATTTCCGGCGACCTCATGAAAGACCCTGTTCTCTTGATTACCGGTCAGACATATGATCGGTTCTTCATCGAAAAGTGGTTCCATGAAGGCCACAATACATGTCCTCAAACCAAGGAAGTCCTTACCGACGTGACTCTTACCCCGAATCGTCTTCTTCGGTCGATGATCTCGCAATGGTGCTTAGATAACAGATTTGAACTGCCAAGGCTTACTTATGAGGAAGAAGTCGACAATGTAACTGAGTCCCATTTAGATGCATTGCTTAACAGGTTGATGTCTTCATCCCTAATAGTTAAAAAAGAAGCTGCAAAGGAGTTGAGAGAGACAACAAGATGGAGCCATGAATTTCGAGCTCTTTTCGCAAAGCTCCCGGCGTCCATTGAGAGATTATTGCATCCTCTGATCTCAATTGGCAAAGTTGATCTCCATCCTGATCTTCGAGAGGATTTGATTACCACTATTCTCAATATCTCAATTTTTGATGATAACAAGAAACATGTAGCGGAGAACCCGTTGGTTCTCCCTTTGTTAATTGAATCCTTACAGTACGGAAATATTGAGGCGACAGCGAATGCAGTTGCAGCTATTTACTCATTATCTCTCAATGAGACAAATAAGATAACGATGGGAAAAGTAGGAATTTTCAAACATCTGATTTCAGTTTTAGACTATGCCCATCCTGTAGTGATAAGAGATGCTGGTTCAGCAATCTACAACCTCTGTACCGTCGTCGAAAACAGAGAGAAGGCAGTTGGCAGTGGAGTAGTTGCCTCCATTTTTCGAAACATCGGTCGAAGCTTATTATTGGTCGATAAGTTGATTTCGATACTTGCACTTCTTTCCACCGATATGAAGGCTATTGACGAGATGTGCAAGTTCGATGCAGTGCCTTGTATGCTGAGGATCATAAGGGAAACTGAGTCCCAACGCATCAAAGAAAATTGTGCATCGATACTCTTTGCAATTTGCACAACTGATCGAAGCCAATTAAGGAAGATCCAGGAGGATGAAAATAAAAATGAGACGATCTTGGAACTTTCAAAGAATGGTAATTCAAGGGCAAGGAGAAAGGCTACCGGCATACTCGATAGGATGCATAGAGCTGCTTCAAGAACTCACACTTCCTAA

Protein sequence

MAMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGNSRARRKATGILDRMHRAASRTHTS
Homology
BLAST of PI0024609 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.8e-94
Identity = 197/446 (44.17%), Postives = 292/446 (65.47%), Query Frame = 0

Query: 6   LKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTPIVPDK 65
           LK+++K+LL +I +E+D      D+    L+AL+   MR     + ++     T   P++
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMR--KMAKSSSLEMLETVSCPEE 79

Query: 66  FRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRLLRSMI 125
           FRCP+S +LM+DPV+L +GQTYD+ FI+KW   G+ TCP+T++VL    LTPN L+R MI
Sbjct: 80  FRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIREMI 139

Query: 126 SQWCLDNRFEL-----PRLTYEEEVDNVTESHL-DALLNRLMSSSLIVKKEAAKELRETT 185
           S+WC  N  E      P L  E+E    ++  + ++LL ++ SS+L  +K AAKELR  T
Sbjct: 140 SKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRLLT 199

Query: 186 RWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDD-NKKHVAE 245
           R   EFRALF + P  I RL++PL+     D    L+ED++TT+LNISI DD NKK V E
Sbjct: 200 RKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKLVCE 259

Query: 246 NPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVV 305
           NP V+PLLI++L+ G +   +NA AAI++LS  ++NK+ +GK GI K LI +L+  +P+ 
Sbjct: 260 NPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPLA 319

Query: 306 IRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDE 365
           I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L++ILA+L T  KA++E
Sbjct: 320 IKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKAVEE 379

Query: 366 MCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKN 425
           + +   V  +L+I RE+E +R KEN   IL  IC +DR++ ++I+E+EN + TI +LS+ 
Sbjct: 380 LGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSRE 439

Query: 426 GNSRARRKATGILDRMHRAASRTHTS 445
           G SRA+RKA GILDR+ +A + THT+
Sbjct: 440 GTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of PI0024609 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 7.3e-64
Identity = 166/451 (36.81%), Postives = 268/451 (59.42%), Query Frame = 0

Query: 4   DHLKKDLKQLLYRII---------EEEDFDS--SAADEAIHILTALKFCLMRAPSSPEPT 63
           D L+++L+++L  I+         E E F     A DEA+ ILT L+    + P S    
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVESKIPES---- 69

Query: 64  ATSGTNTPI-VPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWF-HEGHNTCPQTKEVL 123
                 +P+ VP +F C +S  +M +PV++ +GQTY++ +I +W  HE   TCP+TK+VL
Sbjct: 70  ----DISPVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKHE--RTCPKTKQVL 129

Query: 124 TDVTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKK- 183
           +     PN L+  +I+QWCL N+++  + + +E V  +  S ++ALL R+ SSS +  + 
Sbjct: 130 SHRLWIPNHLISDLITQWCLVNKYDHQKPS-DELVAELFTSDIEALLQRVSSSSSVADQI 189

Query: 184 EAAKELRETTRWSHEFRALF-AKLPASIERLLHPLISIGK-VDLHPDLREDLITTILNIS 243
           EAAKELR  T+     R  F A +  SI RLL PL ++ + VD   +L+E+++T + N+S
Sbjct: 190 EAAKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLS 249

Query: 244 IFDDNKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHL 303
           I + NK  +AEN LV+PLL +SL+ G  E   NA A + SLS  ++NKI +G     K L
Sbjct: 250 ILESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKAL 309

Query: 304 ISVLDYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILA 363
           I +++    +  ++A S ++NLC V+EN+ K V +G++ +  + I ++   VD+L+S+LA
Sbjct: 310 IDLIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKI-KAGSNVDELLSLLA 369

Query: 364 LLSTDMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAIC--TTDRSQLRKIQED 423
           L+ST  +A++EM K   +  +  I+R+  S    EN   I+F +     DRS+L+ + E+
Sbjct: 370 LISTHNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEE 429

Query: 424 ENKNETILELSKNGNSRARRKATGILDRMHR 437
           EN++ T  +L+K G+ RA RKA GIL  + R
Sbjct: 430 ENQHGTFTKLAKQGSVRAARKAQGILQWIKR 448

BLAST of PI0024609 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.3e-57
Identity = 145/446 (32.51%), Postives = 249/446 (55.83%), Query Frame = 0

Query: 3   MDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTN---- 62
           M  +K +   L   +I          D+   +L  LK C++    + +   T   +    
Sbjct: 165 MADMKNESIALHNMVISTAGEPDGCVDQMSSLLKKLKDCVVTEDHANDALTTRSASIKHR 224

Query: 63  TPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPN 122
           +PI+PD+FRCPIS +LM+DPV++ +GQTY+R  I+KW   GH TCP+T++ L+  +LTPN
Sbjct: 225 SPIIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPN 284

Query: 123 RLLRSMISQWCLDNRFELPRLTYEEEVDNVTESH------LDALLNRLMSSSLIVKKEAA 182
            +L+S+ISQWC  N  ELP+           +S       L +L+NRL S +   ++ AA
Sbjct: 285 FVLKSLISQWCEANGIELPKNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAA 344

Query: 183 KELRETTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDN 242
            E+R   + +   R   A+  A    LL  L+S       P  +E  +T +LN+SI ++N
Sbjct: 345 GEIRLLAKRNVNNRICIAE--AGAIPLLVNLLS----SSDPRTQEHAVTALLNLSIHENN 404

Query: 243 KKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLD 302
           K  + ++   +P ++E L+ G++E   NA A ++SLS+ + NK+T+G  G    LI++L 
Sbjct: 405 KASIVDSH-AIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLC 464

Query: 303 YAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFR-NIGRSLLLVDKLISILALLST 362
              P   +DA +AI+NLC    N+ +AV +G+V  +    +  +  ++D+ +S+L++L+ 
Sbjct: 465 DGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAG 524

Query: 363 DMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNET 422
           + +    + + + +P ++ +I+ T S R +EN A+IL+ +C+ D  Q    +      + 
Sbjct: 525 NPEGKIVIARSEPIPPLVEVIK-TGSPRNRENAAAILWLLCSADTEQTLAAKA-AGVEDA 584

Query: 423 ILELSKNGNSRARRKATGILDRMHRA 438
           + ELS+ G  RA+RKA+ IL+ MH+A
Sbjct: 585 LKELSETGTDRAKRKASSILELMHQA 601

BLAST of PI0024609 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.0e-54
Identity = 142/448 (31.70%), Postives = 244/448 (54.46%), Query Frame = 0

Query: 3   MDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTA----TSGTN 62
           +D LKK+   +    +  +       +    +L  L   +    S P+P+      S   
Sbjct: 185 IDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHR 244

Query: 63  TPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPN 122
           +P++P+ FRCPIS +LMKDPV++ TGQTY+R  I+KW   GH TCP+++E L    LTPN
Sbjct: 245 SPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPN 304

Query: 123 RLLRSMISQWCLDNRFELPR---LTYEEEVDNVTESHLD-----ALLNRLMSSSLIVKKE 182
            +L+S+I+ WC  N  ELP+        ++   + S  D     +LL +L + +   ++ 
Sbjct: 305 YVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRA 364

Query: 183 AAKELRETTRWSHEFRALFAK---LPASIERLLHPLISIGKVDLHPDLREDLITTILNIS 242
           AA ELR   + + + R   A+   +P  +E L  P          P  +E  +T +LN+S
Sbjct: 365 AAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSP---------DPRTQEHSVTALLNLS 424

Query: 243 IFDDNKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHL 302
           I + NK  + +   +   ++E L+ G++EA  NA A ++SLS+ + NK+ +G  G  + L
Sbjct: 425 INEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQAL 484

Query: 303 ISVLDYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVV---ASIFRNIGRSLLLVDKLIS 362
           IS+L+       +DA +AI+NLC    N+ +AV  G+V     + ++ G    +VD+ ++
Sbjct: 485 ISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGG--MVDEALA 544

Query: 363 ILALLSTDMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQE 422
           ILA+LST+ +    + + +++P ++ IIR T S R +EN A+IL+ +C  +  +L  +  
Sbjct: 545 ILAILSTNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAILWYLCIGNIERL-NVAR 604

Query: 423 DENKNETILELSKNGNSRARRKATGILD 433
           +   +  + EL++NG  RA+RKA  +L+
Sbjct: 605 EVGADVALKELTENGTDRAKRKAASLLE 618

BLAST of PI0024609 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 208.4 bits (529), Expect = 1.7e-52
Identity = 131/379 (34.56%), Postives = 224/379 (59.10%), Query Frame = 0

Query: 62  VPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRLL 121
           +P  F CP+S +LMKDPV++ TGQTY+R +I++W   G+ TCP+T++ L + TLTPN +L
Sbjct: 241 IPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLENFTLTPNYVL 300

Query: 122 RSMISQWCLDNRFELPRLTYEEEVDNVTE-SHLDALLNRLMSSSLIVKKEAAKELRETTR 181
           RS+IS+WC ++  E P         N  + S + AL+ RL S S   ++ A  E+R  ++
Sbjct: 301 RSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLSSRSTEDRRNAVSEIRSLSK 360

Query: 182 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 241
            S + R L A+  A I  L++ L S          +E+ IT +LN+SI+++NK+ +    
Sbjct: 361 RSTDNRILIAEAGA-IPVLVNLLTSEDVA-----TQENAITCVLNLSIYENNKELIMFAG 420

Query: 242 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 301
            V   +++ L+ G +EA  NA A ++SLSL + NKI +G  G    L+ +L+   P   +
Sbjct: 421 AVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKK 480

Query: 302 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSL--LLVDKLISILALLSTDMKAIDE 361
           DA +A++NLC    N+ +AV +G+V ++ + +  S    +VD+ ++IL++L+ +  A   
Sbjct: 481 DAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLANNQDAKSA 540

Query: 362 MCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKN 421
           + K + +P ++ I+ +T+  R +EN A+IL ++C  D  +L  I         +++LSKN
Sbjct: 541 IVKANTLPALIGIL-QTDQTRNRENAAAILLSLCKRDTEKLITIGR-LGAVVPLMDLSKN 600

Query: 422 GNSRARRKATGILDRMHRA 438
           G  R +RKA  +L+ + +A
Sbjct: 601 GTERGKRKAISLLELLRKA 610

BLAST of PI0024609 vs. ExPASy TrEMBL
Match: A0A5A7UZN6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00400 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.9e-220
Identity = 398/444 (89.64%), Postives = 418/444 (94.14%), Query Frame = 0

Query: 1   MAMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTP 60
           MAMD LKKDLKQ+LYRII+EEDFDSSAAD+AI ILT+LKF LM APSS E    SG+ T 
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTL 60

Query: 61  IVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRL 120
           I+PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTD+TLTPNRL
Sbjct: 61  IIPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNRFELPRL+YEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 240
            SHEFRALFAKLP S+ERLLHPLISIGKVDLHPDL+EDLITTILNIS+FDDNK+HV ENP
Sbjct: 181 CSHEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENP 240

Query: 241 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 300
           LVLPLLIESLQ+G+IE TANAVAAIYSLS +E NKITMGKVGIFKHLIS+LDYAHP V R
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTR 300

Query: 301 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMC 360
           DAGSAIYNLCT VENREKAVGSGVVA+IFRNI RSLLLVDKLISILALL TD+KAIDEMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGN 420
           KFD VPCMLR IRETESQRIKENCASILFAICTTD+SQLRKIQEDENKNETILELSKNGN
Sbjct: 361 KFDVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGN 420

Query: 421 SRARRKATGILDRMHRAASRTHTS 445
           SRA RKATGIL+RM RAASRTHTS
Sbjct: 421 SRASRKATGILERMQRAASRTHTS 444

BLAST of PI0024609 vs. ExPASy TrEMBL
Match: A0A1S3B4X7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.9e-220
Identity = 398/444 (89.64%), Postives = 418/444 (94.14%), Query Frame = 0

Query: 1   MAMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTP 60
           MAMD LKKDLKQ+LYRII+EEDFDSSAAD+AI ILT+LKF LM APSS E    SG+ T 
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTL 60

Query: 61  IVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRL 120
           I+PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTD+TLTPNRL
Sbjct: 61  IIPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNRFELPRL+YEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 240
            SHEFRALFAKLP S+ERLLHPLISIGKVDLHPDL+EDLITTILNIS+FDDNK+HV ENP
Sbjct: 181 CSHEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENP 240

Query: 241 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 300
           LVLPLLIESLQ+G+IE TANAVAAIYSLS +E NKITMGKVGIFKHLIS+LDYAHP V R
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTR 300

Query: 301 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMC 360
           DAGSAIYNLCT VENREKAVGSGVVA+IFRNI RSLLLVDKLISILALL TD+KAIDEMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGN 420
           KFD VPCMLR IRETESQRIKENCASILFAICTTD+SQLRKIQEDENKNETILELSKNGN
Sbjct: 361 KFDVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGN 420

Query: 421 SRARRKATGILDRMHRAASRTHTS 445
           SRA RKATGIL+RM RAASRTHTS
Sbjct: 421 SRASRKATGILERMQRAASRTHTS 444

BLAST of PI0024609 vs. ExPASy TrEMBL
Match: A0A0A0LHP1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G080180 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.4e-220
Identity = 398/444 (89.64%), Postives = 416/444 (93.69%), Query Frame = 0

Query: 1   MAMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTP 60
           MAMD LKKDLKQ+LYRII+E+DFDSSAADEAI ILTALKF LM A SS E T  SG+NT 
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60

Query: 61  IVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRL 120
           I PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQT EVLTD+TLTPNRL
Sbjct: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTNEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNR ELPRL+YEEEVDNVTESHLDALL +L+SSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 240
           WSHEFRALFAKLP S+ERLLHPL+SIGKVDLHPDL EDLITTILNIS+FDDNKKHVAENP
Sbjct: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240

Query: 241 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 300
           LVLPLLIESLQ+G+IE TANAVAAIYSLS NE NKITMGKVG+FKHLIS+LDYAHP VIR
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300

Query: 301 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMC 360
           DAGSAIYNLCT VENREKAVGSGVVA+IFRNIGRSLLLVDKLI ILALL TD KAI+EMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGN 420
           KFDAVPCMLRIIRETESQRIKENCASILFAICTTD+SQLRKIQEDENK ETILELSK GN
Sbjct: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKYETILELSKIGN 420

Query: 421 SRARRKATGILDRMHRAASRTHTS 445
           SRARRKATGILDRMH AA RTHTS
Sbjct: 421 SRARRKATGILDRMHSAAPRTHTS 444

BLAST of PI0024609 vs. ExPASy TrEMBL
Match: A0A6J1HMF9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 9.4e-147
Identity = 277/453 (61.15%), Postives = 349/453 (77.04%), Query Frame = 0

Query: 2   AMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTAT------- 61
           +++ LKKD+K LL RI++++DF+ S ADEAI  L +LK   M A ++    A        
Sbjct: 6   SLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAAPPLES 65

Query: 62  ---SGTNTPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLT 121
              SG    ++PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL 
Sbjct: 66  GGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVLH 125

Query: 122 DVTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEA 181
           D+TLTPNR LRSMI +WC+DN+ ELP+LTYEE V + TESHLD LLN+L+SSSL  +K A
Sbjct: 126 DMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKLA 185

Query: 182 AKELRETTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDD 241
           AKELR+  +W+HEFR+LFAKLP SIERLLHP IS+  V+LHPDL+EDLITT+LNIS+  D
Sbjct: 186 AKELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSGD 245

Query: 242 NKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVL 301
           NKK + EN LVL LLI+SLQYG+ EA ANA AAI+SLSL + NKI +G  G  KHL+ +L
Sbjct: 246 NKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGLL 305

Query: 302 DYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLST 361
           D  HPVV+RDA SAI++LCT   N+EKAV SGVV S+ RNI    +LVD+L+S+LALLS 
Sbjct: 306 DEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLSN 365

Query: 362 DMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNET 421
           +MKAI  MCK +AV CMLR+IRE+ SQ +KENC SIL  I +T RS+L++IQEDEN N  
Sbjct: 366 NMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANGM 425

Query: 422 ILELSKNGNSRARRKATGILDRMHRAASRTHTS 445
           I +LS+NGNSRA+RKA  +L+++ RA S THT+
Sbjct: 426 ISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of PI0024609 vs. ExPASy TrEMBL
Match: A0A6J1FC00 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.7e-146
Identity = 276/452 (61.06%), Postives = 348/452 (76.99%), Query Frame = 0

Query: 2   AMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTAT------- 61
           +++ LKKD+K+LL RI++++DF+ S ADEAI  L +LK   M A S+    A        
Sbjct: 6   SLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAASAAAAAAAAAPVESG 65

Query: 62  --SGTNTPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTD 121
             S     ++PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL D
Sbjct: 66  GDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVLHD 125

Query: 122 VTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAA 181
           +TLTPNR LRSMI +WC+DN+ ELP+LTYEE V + TESHLD LLN+L+SSSL  +K AA
Sbjct: 126 MTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKLAA 185

Query: 182 KELRETTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDN 241
           KELR+  +W+HEFR+LFAKLP SIERLLHP IS+  V+LHPDL+EDLITT+LNIS+  DN
Sbjct: 186 KELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSGDN 245

Query: 242 KKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLD 301
           KK + EN LVL LLI+SLQYG+ EA ANA AAI+SLSL + NKI +G  G  KHL+ +LD
Sbjct: 246 KKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGLLD 305

Query: 302 YAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTD 361
             HPVV+RDA SAI+ LCT   N+EKAV SGVV S+ RNI    +LVD+L+S+LALLS +
Sbjct: 306 EGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLSNN 365

Query: 362 MKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETI 421
           MKAI  MCK +AV CML++IRE+ SQ +KENC SIL  I +T RS+L++IQEDEN N  I
Sbjct: 366 MKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANGMI 425

Query: 422 LELSKNGNSRARRKATGILDRMHRAASRTHTS 445
            +LS+NGNSRA+RKA  +L+++ RA S THT+
Sbjct: 426 SKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 455

BLAST of PI0024609 vs. NCBI nr
Match: XP_008441805.1 (PREDICTED: U-box domain-containing protein 9-like [Cucumis melo] >KAA0061413.1 U-box domain-containing protein 9-like [Cucumis melo var. makuwa] >TYK28671.1 U-box domain-containing protein 9-like [Cucumis melo var. makuwa])

HSP 1 Score: 774.2 bits (1998), Expect = 5.9e-220
Identity = 398/444 (89.64%), Postives = 418/444 (94.14%), Query Frame = 0

Query: 1   MAMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTP 60
           MAMD LKKDLKQ+LYRII+EEDFDSSAAD+AI ILT+LKF LM APSS E    SG+ T 
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTL 60

Query: 61  IVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRL 120
           I+PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTD+TLTPNRL
Sbjct: 61  IIPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNRFELPRL+YEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 240
            SHEFRALFAKLP S+ERLLHPLISIGKVDLHPDL+EDLITTILNIS+FDDNK+HV ENP
Sbjct: 181 CSHEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENP 240

Query: 241 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 300
           LVLPLLIESLQ+G+IE TANAVAAIYSLS +E NKITMGKVGIFKHLIS+LDYAHP V R
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTR 300

Query: 301 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMC 360
           DAGSAIYNLCT VENREKAVGSGVVA+IFRNI RSLLLVDKLISILALL TD+KAIDEMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGN 420
           KFD VPCMLR IRETESQRIKENCASILFAICTTD+SQLRKIQEDENKNETILELSKNGN
Sbjct: 361 KFDVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGN 420

Query: 421 SRARRKATGILDRMHRAASRTHTS 445
           SRA RKATGIL+RM RAASRTHTS
Sbjct: 421 SRASRKATGILERMQRAASRTHTS 444

BLAST of PI0024609 vs. NCBI nr
Match: XP_004152860.1 (U-box domain-containing protein 9 [Cucumis sativus] >KGN61318.1 hypothetical protein Csa_006758 [Cucumis sativus])

HSP 1 Score: 773.1 bits (1995), Expect = 1.3e-219
Identity = 398/444 (89.64%), Postives = 416/444 (93.69%), Query Frame = 0

Query: 1   MAMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTP 60
           MAMD LKKDLKQ+LYRII+E+DFDSSAADEAI ILTALKF LM A SS E T  SG+NT 
Sbjct: 1   MAMDLLKKDLKQVLYRIIQEQDFDSSAADEAIQILTALKFSLMAASSSQESTRCSGSNTL 60

Query: 61  IVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRL 120
           I PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQT EVLTD+TLTPNRL
Sbjct: 61  IFPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTNEVLTDMTLTPNRL 120

Query: 121 LRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 180
           LRSMISQWCLDNR ELPRL+YEEEVDNVTESHLDALL +L+SSSLIVKKEAAKELRETTR
Sbjct: 121 LRSMISQWCLDNRLELPRLSYEEEVDNVTESHLDALLEKLLSSSLIVKKEAAKELRETTR 180

Query: 181 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 240
           WSHEFRALFAKLP S+ERLLHPL+SIGKVDLHPDL EDLITTILNIS+FDDNKKHVAENP
Sbjct: 181 WSHEFRALFAKLPGSVERLLHPLVSIGKVDLHPDLLEDLITTILNISVFDDNKKHVAENP 240

Query: 241 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 300
           LVLPLLIESLQ+G+IE TANAVAAIYSLS NE NKITMGKVG+FKHLIS+LDYAHP VIR
Sbjct: 241 LVLPLLIESLQHGSIELTANAVAAIYSLSFNEANKITMGKVGVFKHLISLLDYAHPGVIR 300

Query: 301 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMC 360
           DAGSAIYNLCT VENREKAVGSGVVA+IFRNIGRSLLLVDKLI ILALL TD KAI+EMC
Sbjct: 301 DAGSAIYNLCTTVENREKAVGSGVVAAIFRNIGRSLLLVDKLILILALLCTDTKAINEMC 360

Query: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGN 420
           KFDAVPCMLRIIRETESQRIKENCASILFAICTTD+SQLRKIQEDENK ETILELSK GN
Sbjct: 361 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKYETILELSKIGN 420

Query: 421 SRARRKATGILDRMHRAASRTHTS 445
           SRARRKATGILDRMH AA RTHTS
Sbjct: 421 SRARRKATGILDRMHSAAPRTHTS 444

BLAST of PI0024609 vs. NCBI nr
Match: XP_038890637.1 (U-box domain-containing protein 9-like [Benincasa hispida])

HSP 1 Score: 637.1 bits (1642), Expect = 1.1e-178
Identity = 330/444 (74.32%), Postives = 380/444 (85.59%), Query Frame = 0

Query: 3   MDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSS--PEPTATSGTNTP 62
           MD LKKDLK LL RI++E+D + SA DEAI  L ALK   M A +S  PE + +SGTNTP
Sbjct: 1   MDVLKKDLKLLLQRIVDEDDLEFSAVDEAIQKLNALKISKMAASASPIPESSGSSGTNTP 60

Query: 63  IVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRL 122
           IVP++FRCPISG+LM DPVLLITGQTYDRFFIE+WFHEGHNTCP+T EVL+D+++TPNRL
Sbjct: 61  IVPEEFRCPISGELMNDPVLLITGQTYDRFFIEQWFHEGHNTCPRTNEVLSDMSITPNRL 120

Query: 123 LRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTR 182
           LRSMISQWCLDNR   P+L+YEEEVDN TESHLD LLN+LMS S+  K +AAKELRETTR
Sbjct: 121 LRSMISQWCLDNRLVPPKLSYEEEVDNATESHLDELLNKLMSLSITDKIDAAKELRETTR 180

Query: 183 WSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDDNKKHVAENP 242
            SHEFRALFAK P SIERLL+PLIS+ K++L+P L+EDLITT+ NISIFD NKKHVAE+P
Sbjct: 181 LSHEFRALFAKFPKSIERLLYPLISLEKINLYPRLQEDLITTVHNISIFDGNKKHVAEHP 240

Query: 243 LVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVVIR 302
           LVLPLLI+SLQYG+IEA ANA A IY LSLNE NKI +G  G+FKHLI++LD+AH VVIR
Sbjct: 241 LVLPLLIQSLQYGSIEAIANAAATIYLLSLNEANKIMIGNAGVFKHLIALLDHAHLVVIR 300

Query: 303 DAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDEMC 362
           DA SAIYNLCT VENR K VG G VASI RNIGR  LLVD+LISILALLSTD+ AIDEMC
Sbjct: 301 DAASAIYNLCTAVENRVKVVGCGAVASILRNIGRR-LLVDELISILALLSTDVNAIDEMC 360

Query: 363 KFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKNGN 422
           K +AVPCML IIRET+SQR+KENCA +L AICTTD+SQL+KI++DEN+N TI ELSK GN
Sbjct: 361 KLNAVPCMLGIIRETDSQRVKENCALMLLAICTTDQSQLKKIRKDENENGTISELSKIGN 420

Query: 423 SRARRKATGILDRMHRAASRTHTS 445
           SRARRKA+ ILDR+HRA SRTHT+
Sbjct: 421 SRARRKASSILDRLHRAGSRTHTA 443

BLAST of PI0024609 vs. NCBI nr
Match: XP_023538150.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532.3 bits (1370), Expect = 3.9e-147
Identity = 278/454 (61.23%), Postives = 350/454 (77.09%), Query Frame = 0

Query: 2   AMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTAT------- 61
           +++ LKKD+K+LL RI++++DF+ S ADEAI  L +LK   M A ++    A        
Sbjct: 6   SLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAAAAPVE 65

Query: 62  ----SGTNTPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVL 121
               SG    ++PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL
Sbjct: 66  SGGDSGRKALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVL 125

Query: 122 TDVTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKE 181
            D+TLTPNR LRSMI +WC+DN+ ELP+LTYEE V + TESHLD LLN+L+SSSL  +K 
Sbjct: 126 HDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKL 185

Query: 182 AAKELRETTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFD 241
           AAKELR+  +W+HEFR+LFAKLP SIERLLHP IS+  V+LHPDL+EDLITT+LNIS+  
Sbjct: 186 AAKELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSG 245

Query: 242 DNKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISV 301
           DNKK + EN LVL LLI+SLQYG+ EA ANA AAI+SLSL + NKI +G  G  KHL+ +
Sbjct: 246 DNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGL 305

Query: 302 LDYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLS 361
           LD  HPVV+RDA SAI+ LCT   N+EKAV SGVV S+ RNI    +LVD+L+S+LALLS
Sbjct: 306 LDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLS 365

Query: 362 TDMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNE 421
            +MKAI  MCK +AV CMLR+IRE+ SQ +KENC SIL  I +T RS+L++IQEDEN N 
Sbjct: 366 NNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANG 425

Query: 422 TILELSKNGNSRARRKATGILDRMHRAASRTHTS 445
            I +LS+NGNSRA+RKAT +L+++ RA S THT+
Sbjct: 426 MISKLSRNGNSRAKRKATSVLEKLQRAGSITHTA 457

BLAST of PI0024609 vs. NCBI nr
Match: XP_022965721.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 530.0 bits (1364), Expect = 1.9e-146
Identity = 277/453 (61.15%), Postives = 349/453 (77.04%), Query Frame = 0

Query: 2   AMDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTAT------- 61
           +++ LKKD+K LL RI++++DF+ S ADEAI  L +LK   M A ++    A        
Sbjct: 6   SLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAAPPLES 65

Query: 62  ---SGTNTPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLT 121
              SG    ++PD+FRCPISG+LM+DPVLL TGQTYDR FI KWF++GHNTCP+T EVL 
Sbjct: 66  GGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTNEVLH 125

Query: 122 DVTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKKEA 181
           D+TLTPNR LRSMI +WC+DN+ ELP+LTYEE V + TESHLD LLN+L+SSSL  +K A
Sbjct: 126 DMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSDQKLA 185

Query: 182 AKELRETTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDD 241
           AKELR+  +W+HEFR+LFAKLP SIERLLHP IS+  V+LHPDL+EDLITT+LNIS+  D
Sbjct: 186 AKELRQIAKWNHEFRSLFAKLPDSIERLLHP-ISVDHVNLHPDLQEDLITTVLNISLSGD 245

Query: 242 NKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVL 301
           NKK + EN LVL LLI+SLQYG+ EA ANA AAI+SLSL + NKI +G  G  KHL+ +L
Sbjct: 246 NKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLVGLL 305

Query: 302 DYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLST 361
           D  HPVV+RDA SAI++LCT   N+EKAV SGVV S+ RNI    +LVD+L+S+LALLS 
Sbjct: 306 DEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEG-VLVDELVSVLALLSN 365

Query: 362 DMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNET 421
           +MKAI  MCK +AV CMLR+IRE+ SQ +KENC SIL  I +T RS+L++IQEDEN N  
Sbjct: 366 NMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENANGM 425

Query: 422 ILELSKNGNSRARRKATGILDRMHRAASRTHTS 445
           I +LS+NGNSRA+RKA  +L+++ RA S THT+
Sbjct: 426 ISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of PI0024609 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 347.8 bits (891), Expect = 1.3e-95
Identity = 197/446 (44.17%), Postives = 292/446 (65.47%), Query Frame = 0

Query: 6   LKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTATSGTNTPIVPDK 65
           LK+++K+LL +I +E+D      D+    L+AL+   MR     + ++     T   P++
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMR--KMAKSSSLEMLETVSCPEE 79

Query: 66  FRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPNRLLRSMI 125
           FRCP+S +LM+DPV+L +GQTYD+ FI+KW   G+ TCP+T++VL    LTPN L+R MI
Sbjct: 80  FRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIREMI 139

Query: 126 SQWCLDNRFEL-----PRLTYEEEVDNVTESHL-DALLNRLMSSSLIVKKEAAKELRETT 185
           S+WC  N  E      P L  E+E    ++  + ++LL ++ SS+L  +K AAKELR  T
Sbjct: 140 SKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRLLT 199

Query: 186 RWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDD-NKKHVAE 245
           R   EFRALF + P  I RL++PL+     D    L+ED++TT+LNISI DD NKK V E
Sbjct: 200 RKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKLVCE 259

Query: 246 NPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHPVV 305
           NP V+PLLI++L+ G +   +NA AAI++LS  ++NK+ +GK GI K LI +L+  +P+ 
Sbjct: 260 NPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPLA 319

Query: 306 IRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAIDE 365
           I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L++ILA+L T  KA++E
Sbjct: 320 IKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKAVEE 379

Query: 366 MCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELSKN 425
           + +   V  +L+I RE+E +R KEN   IL  IC +DR++ ++I+E+EN + TI +LS+ 
Sbjct: 380 LGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSRE 439

Query: 426 GNSRARRKATGILDRMHRAASRTHTS 445
           G SRA+RKA GILDR+ +A + THT+
Sbjct: 440 GTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of PI0024609 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 247.3 bits (630), Expect = 2.3e-65
Identity = 148/328 (45.12%), Postives = 216/328 (65.85%), Query Frame = 0

Query: 124 MISQWCLDNRFEL-----PRLTYEEEVDNVTESHL-DALLNRLMSSSLIVKKEAAKELRE 183
           MIS+WC  N  E      P L  E+E    ++  + ++LL ++ SS+L  +K AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 184 TTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDD-NKKHV 243
            TR   EFRALF + P  I RL++PL+     D    L+ED++TT+LNISI DD NKK V
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKLV 120

Query: 244 AENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHP 303
            ENP V+PLLI++L+ G +   +NA AAI++LS  ++NK+ +GK GI K LI +L+  +P
Sbjct: 121 CENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNP 180

Query: 304 VVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAI 363
           + I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L++ILA+L T  KA+
Sbjct: 181 LAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKAV 240

Query: 364 DEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELS 423
           +E+ +   V  +L+I RE+E +R KEN   IL  IC +DR++ ++I+E+EN + TI +LS
Sbjct: 241 EELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLS 300

Query: 424 KNGNSRARRKATGILDRMHRAASRTHTS 445
           + G SRA+RKA GILDR+ +A + THT+
Sbjct: 301 REGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of PI0024609 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 247.3 bits (630), Expect = 2.3e-65
Identity = 148/328 (45.12%), Postives = 216/328 (65.85%), Query Frame = 0

Query: 124 MISQWCLDNRFEL-----PRLTYEEEVDNVTESHL-DALLNRLMSSSLIVKKEAAKELRE 183
           MIS+WC  N  E      P L  E+E    ++  + ++LL ++ SS+L  +K AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 184 TTRWSHEFRALFAKLPASIERLLHPLISIGKVDLHPDLREDLITTILNISIFDD-NKKHV 243
            TR   EFRALF + P  I RL++PL+     D    L+ED++TT+LNISI DD NKK V
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPD--EKLQEDVVTTLLNISIHDDSNKKLV 120

Query: 244 AENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHLISVLDYAHP 303
            ENP V+PLLI++L+ G +   +NA AAI++LS  ++NK+ +GK GI K LI +L+  +P
Sbjct: 121 CENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNP 180

Query: 304 VVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILALLSTDMKAI 363
           + I+D  +AI+ LC   ENR +AV  G V  + + I    L VD+L++ILA+L T  KA+
Sbjct: 181 LAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNG-LYVDELLAILAMLVTHWKAV 240

Query: 364 DEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQEDENKNETILELS 423
           +E+ +   V  +L+I RE+E +R KEN   IL  IC +DR++ ++I+E+EN + TI +LS
Sbjct: 241 EELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLS 300

Query: 424 KNGNSRARRKATGILDRMHRAASRTHTS 445
           + G SRA+RKA GILDR+ +A + THT+
Sbjct: 301 REGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of PI0024609 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 5.2e-65
Identity = 166/451 (36.81%), Postives = 268/451 (59.42%), Query Frame = 0

Query: 4   DHLKKDLKQLLYRII---------EEEDFDS--SAADEAIHILTALKFCLMRAPSSPEPT 63
           D L+++L+++L  I+         E E F     A DEA+ ILT L+    + P S    
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVESKIPES---- 69

Query: 64  ATSGTNTPI-VPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWF-HEGHNTCPQTKEVL 123
                 +P+ VP +F C +S  +M +PV++ +GQTY++ +I +W  HE   TCP+TK+VL
Sbjct: 70  ----DISPVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKHE--RTCPKTKQVL 129

Query: 124 TDVTLTPNRLLRSMISQWCLDNRFELPRLTYEEEVDNVTESHLDALLNRLMSSSLIVKK- 183
           +     PN L+  +I+QWCL N+++  + + +E V  +  S ++ALL R+ SSS +  + 
Sbjct: 130 SHRLWIPNHLISDLITQWCLVNKYDHQKPS-DELVAELFTSDIEALLQRVSSSSSVADQI 189

Query: 184 EAAKELRETTRWSHEFRALF-AKLPASIERLLHPLISIGK-VDLHPDLREDLITTILNIS 243
           EAAKELR  T+     R  F A +  SI RLL PL ++ + VD   +L+E+++T + N+S
Sbjct: 190 EAAKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLS 249

Query: 244 IFDDNKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHL 303
           I + NK  +AEN LV+PLL +SL+ G  E   NA A + SLS  ++NKI +G     K L
Sbjct: 250 ILESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKAL 309

Query: 304 ISVLDYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVVASIFRNIGRSLLLVDKLISILA 363
           I +++    +  ++A S ++NLC V+EN+ K V +G++ +  + I ++   VD+L+S+LA
Sbjct: 310 IDLIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKI-KAGSNVDELLSLLA 369

Query: 364 LLSTDMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAIC--TTDRSQLRKIQED 423
           L+ST  +A++EM K   +  +  I+R+  S    EN   I+F +     DRS+L+ + E+
Sbjct: 370 LISTHNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEE 429

Query: 424 ENKNETILELSKNGNSRARRKATGILDRMHR 437
           EN++ T  +L+K G+ RA RKA GIL  + R
Sbjct: 430 ENQHGTFTKLAKQGSVRAARKAQGILQWIKR 448

BLAST of PI0024609 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 213.8 bits (543), Expect = 2.9e-55
Identity = 142/448 (31.70%), Postives = 244/448 (54.46%), Query Frame = 0

Query: 3   MDHLKKDLKQLLYRIIEEEDFDSSAADEAIHILTALKFCLMRAPSSPEPTA----TSGTN 62
           +D LKK+   +    +  +       +    +L  L   +    S P+P+      S   
Sbjct: 185 IDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHR 244

Query: 63  TPIVPDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDVTLTPN 122
           +P++P+ FRCPIS +LMKDPV++ TGQTY+R  I+KW   GH TCP+++E L    LTPN
Sbjct: 245 SPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPN 304

Query: 123 RLLRSMISQWCLDNRFELPR---LTYEEEVDNVTESHLD-----ALLNRLMSSSLIVKKE 182
            +L+S+I+ WC  N  ELP+        ++   + S  D     +LL +L + +   ++ 
Sbjct: 305 YVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRA 364

Query: 183 AAKELRETTRWSHEFRALFAK---LPASIERLLHPLISIGKVDLHPDLREDLITTILNIS 242
           AA ELR   + + + R   A+   +P  +E L  P          P  +E  +T +LN+S
Sbjct: 365 AAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSP---------DPRTQEHSVTALLNLS 424

Query: 243 IFDDNKKHVAENPLVLPLLIESLQYGNIEATANAVAAIYSLSLNETNKITMGKVGIFKHL 302
           I + NK  + +   +   ++E L+ G++EA  NA A ++SLS+ + NK+ +G  G  + L
Sbjct: 425 INEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQAL 484

Query: 303 ISVLDYAHPVVIRDAGSAIYNLCTVVENREKAVGSGVV---ASIFRNIGRSLLLVDKLIS 362
           IS+L+       +DA +AI+NLC    N+ +AV  G+V     + ++ G    +VD+ ++
Sbjct: 485 ISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGG--MVDEALA 544

Query: 363 ILALLSTDMKAIDEMCKFDAVPCMLRIIRETESQRIKENCASILFAICTTDRSQLRKIQE 422
           ILA+LST+ +    + + +++P ++ IIR T S R +EN A+IL+ +C  +  +L  +  
Sbjct: 545 ILAILSTNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAILWYLCIGNIERL-NVAR 604

Query: 423 DENKNETILELSKNGNSRARRKATGILD 433
           +   +  + EL++NG  RA+RKA  +L+
Sbjct: 605 EVGADVALKELTENGTDRAKRKAASLLE 618

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRT01.8e-9444.17U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S... [more]
Q3E9F77.3e-6436.81Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q5VRH92.3e-5732.51U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q8VZ404.0e-5431.70U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q8GUG91.7e-5234.56U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7UZN62.9e-22089.64RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B4X72.9e-22089.64RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 ... [more]
A0A0A0LHP16.4e-22089.64RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G080180 PE... [more]
A0A6J1HMF99.4e-14761.15RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 P... [more]
A0A6J1FC002.7e-14661.06RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033... [more]
Match NameE-valueIdentityDescription
XP_008441805.15.9e-22089.64PREDICTED: U-box domain-containing protein 9-like [Cucumis melo] >KAA0061413.1 U... [more]
XP_004152860.11.3e-21989.64U-box domain-containing protein 9 [Cucumis sativus] >KGN61318.1 hypothetical pro... [more]
XP_038890637.11.1e-17874.32U-box domain-containing protein 9-like [Benincasa hispida][more]
XP_023538150.13.9e-14761.23U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo][more]
XP_022965721.11.9e-14661.15U-box domain-containing protein 9-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G07360.11.3e-9544.17plant U-box 9 [more]
AT3G07360.22.3e-6545.12plant U-box 9 [more]
AT3G07360.32.3e-6545.12plant U-box 9 [more]
AT5G18320.15.2e-6536.81ARM repeat superfamily protein [more]
AT3G54850.12.9e-5531.70plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 65..128
e-value: 8.4E-28
score: 108.4
IPR003613U box domainPFAMPF04564U-boxcoord: 62..133
e-value: 6.4E-19
score: 67.9
IPR003613U box domainPROSITEPS51698U_BOXcoord: 61..135
score: 38.188652
IPR000225ArmadilloSMARTSM00185arm_5coord: 229..270
e-value: 70.0
score: 6.7
coord: 271..311
e-value: 0.16
score: 21.0
coord: 352..393
e-value: 11.0
score: 13.1
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 143..441
e-value: 4.7E-28
score: 100.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 62..136
e-value: 2.4E-26
score: 93.6
NoneNo IPR availablePANTHERPTHR23315:SF265U-BOX DOMAIN-CONTAINING PROTEIN 46-RELATEDcoord: 3..438
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 3..438
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 66..108
e-value: 4.06941E-22
score: 86.9038
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 62..137
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 152..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0024609.1PI0024609.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity