PI0024325 (gene) Melon (PI 482460) v1

Overview


Name	PI0024325
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase
Location	chr01: 10895612 .. 10896217 (-)
RNA-Seq Expression	PI0024325
Synteny	PI0024325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTCGAAGAGGAGGAATCCCTGCCACGGGAGACAATCGTGAGCAAGAAGAGGTGGAAGAGAACATCACCCTCTCTCTGAGGACATCCACGGTGCGCTTGCTAGCCGTCGAAGACTCCCTGGGAGATTTACACGAAAAGTTTGTTAGAGTAATGGATACTTTGGAGACACTGACTCGAATGATGGACGGACTACCGGCTCTGGCAAGAATTGAAGCCAATGTAAATAATAACCGGAACGACAAAAACAGAGGAGGCCGACGAGCTCGAAGGAATTTTAGAATTTTGCCCAATCAAAGGAATGATCAAAGAAGGAGGCCAATGGAGAATCTGTTGCGATATGCCGACGATGATTCAATGGAAGAATATGAAGCTTGGAAAAATGGGCAAGAATGTGATTCTTCCAGCGGTGATGAGCAAGGAAATATTTGGAATGATAATGGAGATATTCGAATGAGACGAGTTTATAGAGGACATGAAGCACGAAGGGAAGTTCATCATGATTATAAGATGAAAATTGATCTCCAACCTATGATGGTAAGCGTGATATTGAATCTTTTTTGGATTGGATTAAAAATACTGAAAATTTCTTCAATTATATAA

mRNA sequence

ATGGCTGGTCGAAGAGGAGGAATCCCTGCCACGGGAGACAATCGTGAGCAAGAAGAGGTGGAAGAGAACATCACCCTCTCTCTGAGGACATCCACGGTGCGCTTGCTAGCCGTCGAAGACTCCCTGGGAGATTTACACGAAAAGTTTGTTAGAGTAATGGATACTTTGGAGACACTGACTCGAATGATGGACGGACTACCGGCTCTGGCAAGAATTGAAGCCAATGTAAATAATAACCGGAACGACAAAAACAGAGGAGGCCGACGAGCTCGAAGGAATTTTAGAATTTTGCCCAATCAAAGGAATGATCAAAGAAGGAGGCCAATGGAGAATCTGTTGCGATATGCCGACGATGATTCAATGGAAGAATATGAAGCTTGGAAAAATGGGCAAGAATGTGATTCTTCCAGCGGTGATGAGCAAGGAAATATTTGGAATGATAATGGAGATATTCGAATGAGACGAGTTTATAGAGGACATGAAGCACGAAGGGAAGTTCATCATGATTATAAGATGAAAATTGATCTCCAACCTATGATGGTAAGCGTGATATTGAATCTTTTTTGGATTGGATTAAAAATACTGAAAATTTCTTCAATTATATAA

Coding sequence (CDS)

ATGGCTGGTCGAAGAGGAGGAATCCCTGCCACGGGAGACAATCGTGAGCAAGAAGAGGTGGAAGAGAACATCACCCTCTCTCTGAGGACATCCACGGTGCGCTTGCTAGCCGTCGAAGACTCCCTGGGAGATTTACACGAAAAGTTTGTTAGAGTAATGGATACTTTGGAGACACTGACTCGAATGATGGACGGACTACCGGCTCTGGCAAGAATTGAAGCCAATGTAAATAATAACCGGAACGACAAAAACAGAGGAGGCCGACGAGCTCGAAGGAATTTTAGAATTTTGCCCAATCAAAGGAATGATCAAAGAAGGAGGCCAATGGAGAATCTGTTGCGATATGCCGACGATGATTCAATGGAAGAATATGAAGCTTGGAAAAATGGGCAAGAATGTGATTCTTCCAGCGGTGATGAGCAAGGAAATATTTGGAATGATAATGGAGATATTCGAATGAGACGAGTTTATAGAGGACATGAAGCACGAAGGGAAGTTCATCATGATTATAAGATGAAAATTGATCTCCAACCTATGATGGTAAGCGTGATATTGAATCTTTTTTGGATTGGATTAAAAATACTGAAAATTTCTTCAATTATATAA

Protein sequence

MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLTRMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDSMEEYEAWKNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGHEARREVHHDYKMKIDLQPMMVSVILNLFWIGLKILKISSII

Homology

[Close]

BLAST of PI0024325 vs. ExPASy TrEMBL
Match: A0A5D3CJ99 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00470 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.2e-77
Identity = 154/176 (87.50%), Postives = 162/176 (92.05%), Query Frame = 0


Query: 1   MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLT 60
           MAGRR GIPATGDNREQEEVEEN  LS RTSTV  LAVEDSLGDLHEKF RVMDTLETLT
Sbjct: 1   MAGRREGIPATGDNREQEEVEENAALSPRTSTVCFLAVEDSLGDLHEKFDRVMDTLETLT 60

Query: 61  RMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDS 120
           R MDGLPALARIEAN NN+RND NRGGRRARRNFR LPNQRNDQR+RPMEN LRYAD+DS
Sbjct: 61  RRMDGLPALARIEANANNDRNDGNRGGRRARRNFRNLPNQRNDQRKRPMENPLRYADNDS 120

Query: 121 MEEYEAWKNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGHEARREVHHDYKMKIDL 177
           MEEYEAW+NGQECDSSS DEQGNIWNDN D++MR+VYRGHEA+REVHHDYKMKIDL
Sbjct: 121 MEEYEAWQNGQECDSSSSDEQGNIWNDNRDMQMRQVYRGHEAQREVHHDYKMKIDL 176

[Close]

BLAST of PI0024325 vs. ExPASy TrEMBL
Match: A0A5A7UQ86 (RNA-directed DNA polymerase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G001990 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.7e-67
Identity = 137/176 (77.84%), Postives = 155/176 (88.07%), Query Frame = 0


Query: 1   MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLT 60
           MA RRGGIPA GDNREQEEVE   TLS RTSTVRLLAV+D LGDLHEKF R+MD LETLT
Sbjct: 1   MADRRGGIPAAGDNREQEEVEAIATLSPRTSTVRLLAVKDFLGDLHEKFNRMMDNLETLT 60

Query: 61  RMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDS 120
           + MDGLPA ARIEAN+N +RN++NRGGRRA+RNF  +PNQRNDQ RRPME  LRYADDDS
Sbjct: 61  QRMDGLPAPARIEANMNIDRNERNRGGRRAQRNFSNMPNQRNDQIRRPMEMPLRYADDDS 120

Query: 121 MEEYEAWKNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGHEARREVHHDYKMKIDL 177
           +E+ +AW+N QE DSSSGDE+GNIW+DNGDI+MR+ YRGHEARREVHHDYKMK+DL
Sbjct: 121 LEKGDAWQNRQEYDSSSGDERGNIWDDNGDIQMRQAYRGHEARREVHHDYKMKLDL 176

[Close]

BLAST of PI0024325 vs. ExPASy TrEMBL
Match: A0A5A7UR03 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00180 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.6e-51
Identity = 119/145 (82.07%), Postives = 123/145 (84.83%), Query Frame = 0


Query: 9   PATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLTRMMDGLPA 68
           P  G   EQE+VEE  TLS RTSTV LLA EDSLGDLHEKF RVMDTLETLTR MD L A
Sbjct: 294 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 353

Query: 69  LARIEANVNNNR--NDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDSMEEYEA 128
            ARIEANVNNNR  ND NRGGRRARRNFR LPNQR+DQRRRPMEN LRYADD+SMEEYEA
Sbjct: 354 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 413

Query: 129 WKNGQECDSSSGDEQGNIWNDNGDI 152
            +NGQECDSSS DEQGNIWNDNGDI
Sbjct: 414 RQNGQECDSSSSDEQGNIWNDNGDI 438

[Close]

BLAST of PI0024325 vs. ExPASy TrEMBL
Match: A0A5D3DBZ2 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold778G00120 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.6e-51
Identity = 119/145 (82.07%), Postives = 123/145 (84.83%), Query Frame = 0


Query: 9   PATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLTRMMDGLPA 68
           P  G   EQE+VEE  TLS RTSTV LLA EDSLGDLHEKF RVMDTLETLTR MD L A
Sbjct: 205 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 264

Query: 69  LARIEANVNNNR--NDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDSMEEYEA 128
            ARIEANVNNNR  ND NRGGRRARRNFR LPNQR+DQRRRPMEN LRYADD+SMEEYEA
Sbjct: 265 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 324

Query: 129 WKNGQECDSSSGDEQGNIWNDNGDI 152
            +NGQECDSSS DEQGNIWNDNGDI
Sbjct: 325 RQNGQECDSSSSDEQGNIWNDNGDI 349

[Close]

BLAST of PI0024325 vs. ExPASy TrEMBL
Match: A0A5D3CLX2 (DExH-box ATP-dependent RNA helicase DExH7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00630 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 3.1e-49
Identity = 114/150 (76.00%), Postives = 125/150 (83.33%), Query Frame = 0


Query: 1   MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLT 60
           M  RRGGIPA GDNREQEEVEE  TLS RTSTV LLAVEDSLGDLH KF RVMD+LETLT
Sbjct: 232 MVDRRGGIPAVGDNREQEEVEEITTLSPRTSTVCLLAVEDSLGDLHVKFDRVMDSLETLT 291

Query: 61  RMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDS 120
           R MDGLPA ARIEA   N+R D+NRGGRRARRNFR LP+QRNDQRRR ++N LRY +DDS
Sbjct: 292 RRMDGLPAQARIEA---NDRVDRNRGGRRARRNFRNLPDQRNDQRRRLLDNSLRYNNDDS 351

Query: 121 MEEYEAWKNGQECDSSSGDEQGNIWNDNGD 151
            E YEAW++ Q+ DSS GDEQ NIWNDNG+
Sbjct: 352 EENYEAWQDVQDYDSSRGDEQENIWNDNGE 378

[Close]

BLAST of PI0024325 vs. NCBI nr
Match: TYK11204.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 299.3 bits (765), Expect = 2.5e-77
Identity = 154/176 (87.50%), Postives = 162/176 (92.05%), Query Frame = 0


Query: 1   MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLT 60
           MAGRR GIPATGDNREQEEVEEN  LS RTSTV  LAVEDSLGDLHEKF RVMDTLETLT
Sbjct: 1   MAGRREGIPATGDNREQEEVEENAALSPRTSTVCFLAVEDSLGDLHEKFDRVMDTLETLT 60

Query: 61  RMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDS 120
           R MDGLPALARIEAN NN+RND NRGGRRARRNFR LPNQRNDQR+RPMEN LRYAD+DS
Sbjct: 61  RRMDGLPALARIEANANNDRNDGNRGGRRARRNFRNLPNQRNDQRKRPMENPLRYADNDS 120

Query: 121 MEEYEAWKNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGHEARREVHHDYKMKIDL 177
           MEEYEAW+NGQECDSSS DEQGNIWNDN D++MR+VYRGHEA+REVHHDYKMKIDL
Sbjct: 121 MEEYEAWQNGQECDSSSSDEQGNIWNDNRDMQMRQVYRGHEAQREVHHDYKMKIDL 176

[Close]

BLAST of PI0024325 vs. NCBI nr
Match: KAA0057344.1 (RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 263.8 bits (673), Expect = 1.2e-66
Identity = 137/176 (77.84%), Postives = 155/176 (88.07%), Query Frame = 0


Query: 1   MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLT 60
           MA RRGGIPA GDNREQEEVE   TLS RTSTVRLLAV+D LGDLHEKF R+MD LETLT
Sbjct: 1   MADRRGGIPAAGDNREQEEVEAIATLSPRTSTVRLLAVKDFLGDLHEKFNRMMDNLETLT 60

Query: 61  RMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDS 120
           + MDGLPA ARIEAN+N +RN++NRGGRRA+RNF  +PNQRNDQ RRPME  LRYADDDS
Sbjct: 61  QRMDGLPAPARIEANMNIDRNERNRGGRRAQRNFSNMPNQRNDQIRRPMEMPLRYADDDS 120

Query: 121 MEEYEAWKNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGHEARREVHHDYKMKIDL 177
           +E+ +AW+N QE DSSSGDE+GNIW+DNGDI+MR+ YRGHEARREVHHDYKMK+DL
Sbjct: 121 LEKGDAWQNRQEYDSSSGDERGNIWDDNGDIQMRQAYRGHEARREVHHDYKMKLDL 176

[Close]

BLAST of PI0024325 vs. NCBI nr
Match: TYK21018.1 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa])

HSP 1 Score: 211.8 bits (538), Expect = 5.3e-51
Identity = 119/145 (82.07%), Postives = 123/145 (84.83%), Query Frame = 0


Query: 9   PATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLTRMMDGLPA 68
           P  G   EQE+VEE  TLS RTSTV LLA EDSLGDLHEKF RVMDTLETLTR MD L A
Sbjct: 205 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 264

Query: 69  LARIEANVNNNR--NDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDSMEEYEA 128
            ARIEANVNNNR  ND NRGGRRARRNFR LPNQR+DQRRRPMEN LRYADD+SMEEYEA
Sbjct: 265 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 324

Query: 129 WKNGQECDSSSGDEQGNIWNDNGDI 152
            +NGQECDSSS DEQGNIWNDNGDI
Sbjct: 325 RQNGQECDSSSSDEQGNIWNDNGDI 349

[Close]

BLAST of PI0024325 vs. NCBI nr
Match: KAA0056746.1 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa])

HSP 1 Score: 211.8 bits (538), Expect = 5.3e-51
Identity = 119/145 (82.07%), Postives = 123/145 (84.83%), Query Frame = 0


Query: 9   PATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLTRMMDGLPA 68
           P  G   EQE+VEE  TLS RTSTV LLA EDSLGDLHEKF RVMDTLETLTR MD L A
Sbjct: 294 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 353

Query: 69  LARIEANVNNNR--NDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDSMEEYEA 128
            ARIEANVNNNR  ND NRGGRRARRNFR LPNQR+DQRRRPMEN LRYADD+SMEEYEA
Sbjct: 354 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 413

Query: 129 WKNGQECDSSSGDEQGNIWNDNGDI 152
            +NGQECDSSS DEQGNIWNDNGDI
Sbjct: 414 RQNGQECDSSSSDEQGNIWNDNGDI 438

[Close]

BLAST of PI0024325 vs. NCBI nr
Match: TYK11219.1 (DExH-box ATP-dependent RNA helicase DExH7 [Cucumis melo var. makuwa])

HSP 1 Score: 204.9 bits (520), Expect = 6.5e-49
Identity = 114/150 (76.00%), Postives = 125/150 (83.33%), Query Frame = 0


Query: 1   MAGRRGGIPATGDNREQEEVEENITLSLRTSTVRLLAVEDSLGDLHEKFVRVMDTLETLT 60
           M  RRGGIPA GDNREQEEVEE  TLS RTSTV LLAVEDSLGDLH KF RVMD+LETLT
Sbjct: 232 MVDRRGGIPAVGDNREQEEVEEITTLSPRTSTVCLLAVEDSLGDLHVKFDRVMDSLETLT 291

Query: 61  RMMDGLPALARIEANVNNNRNDKNRGGRRARRNFRILPNQRNDQRRRPMENLLRYADDDS 120
           R MDGLPA ARIEA   N+R D+NRGGRRARRNFR LP+QRNDQRRR ++N LRY +DDS
Sbjct: 292 RRMDGLPAQARIEA---NDRVDRNRGGRRARRNFRNLPDQRNDQRRRLLDNSLRYNNDDS 351

Query: 121 MEEYEAWKNGQECDSSSGDEQGNIWNDNGD 151
            E YEAW++ Q+ DSS GDEQ NIWNDNG+
Sbjct: 352 EENYEAWQDVQDYDSSRGDEQENIWNDNGE 378

The following BLAST results are available for this feature:

BLAST of PI0024325 vs.


Match Name	E-value	Identity	Description

BLAST of PI0024325 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CJ99	1.2e-77	87.50	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold22...	[more]
A0A5A7UQ86	5.7e-67	77.84	RNA-directed DNA polymerase-like protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5A7UR03	2.6e-51	82.07	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=...	[more]
A0A5D3DBZ2	2.6e-51	82.07	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=...	[more]
A0A5D3CLX2	3.1e-49	76.00	DExH-box ATP-dependent RNA helicase DExH7 OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of PI0024325 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK11204.1	2.5e-77	87.50	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0057344.1	1.2e-66	77.84	RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa]	[more]
TYK21018.1	5.3e-51	82.07	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa]	[more]
KAA0056746.1	5.3e-51	82.07	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa]	[more]
TYK11219.1	6.5e-49	76.00	DExH-box ATP-dependent RNA helicase DExH7 [Cucumis melo var. makuwa]	[more]

BLAST of PI0024325 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 146..175 score: 6.6868

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0024325.1	PI0024325.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0016310	phosphorylation
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0008270	zinc ion binding