PI0024116 (gene) Melon (PI 482460) v1

Overview
NamePI0024116
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic
Locationchr09: 18874948 .. 18879034 (+)
RNA-Seq ExpressionPI0024116
SyntenyPI0024116
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACACAGTGAACATTCCCTTGTTGCGTTAAACTATACATGGACAATCATCGGGAAGTGCTTTCATTAATTCAATTATTTTTTTGTTTATGTAGTTTTGGTTGGTTAAATGATCCGCTAATCCAACTCGTAATTTTCTCAATGGTGGTGGTTGGAAGTCAAGGTTTGATGTGGCAAGCGGTAGATAACAGTTGACTAGATAGACTTCTATCAGTTTCTGTCGCTGGTAATATCAATAGTAAACTTAGCTTGTATCAGTTTCTATCACTAATAAACTTTTTATCAATATTTATCAATGATGGATCGACTTCTATTAGTCTCTATCAGTGATAAAATTGTATCAATTTCTATCACCGATAGAGACTGATAAACTTTTATCAGTCTCTTGTTATCGATAGATTTCTATTAATTTCTATCGTTGATAGACACTTATAGAATTTTATTGACCTTTATTGACTATAGGCTTGTATCAATTTCTATAATTGATAGTATCAATTTCTATTATTGATAGACTTCTATTAGTCTCTATTAATGATAGATTTCTATCGATTTCTATCACTGATAGATATTGATTGCTTTTGATCAATTTTTATGACTAATCAACCTCTAAATTTTGCTATATCTATAAAAAAAAATTGCATTGTTTTATATTTACAATTAATTGATATATATACTAAACAAATTAGTTGGATTAAATAACAATTTAATTGGTAATGAGAGAGAAGACTAGAGATGAAAAGAAAGGGATGGAAAGCCAAACTTAATGCAAGTAAAAAAAAAAAAACAAGTGGGACATATCATGTGAATGGGAATGGGGTTGGAAATTAGGAACCAAAAAGTTTGGTCCAAATTTTAGTTATGAATCCTTGGAAGACACTCTTTTTAAAATATAATATTTTCACGTGCAATTTTATAAAAAAAAAAGGGGGTTAGTAGTATTTTAAAATCCAAGCAAACCCCAACTACTCATTGTGTTGGTATCTTGGGAAGTTTATATTTCTATGTCAAACTTATAATGATGTTCTTTTTGGAGGGATTTGAGTTTATTATTACATTTTTTTTTTTTACTTTTTCTATTTTATTTTATATAATAAAACCATTTTAAATGGAGAAAAAAAAACATCTGTTGAAATGGAAGAATAACAAATTTGAATATTGTGCATGGAATATGAATATTTTAATTGGTCAAGTTTTTTGGTTGTTTTTTTAATAGAGCTAAATGAACTAAAATATCTTATATATAGCAAAATATTTATCCATATTAAGCCAAAAAATTAAAAAATTTAAAAATTAGAAGATTAAAAAATCTATATCTTCCTCTCATGTTTGATCATAGTAGAAGTGAGCATGATAGATCGAAAAAGCGAGTCGATTGACCAAAGCCAATCGAACCGAGGACGATCGGTCAAGATAAGAAAGGGTTTGGACGATGTTGGTTTGAGAAAAATTTAAAACAACGTGTTTAAAAAAATTGAAAGGCTTAAATCGACTAATCGATCGACCAACAATCAATTTGTACTACTTCAAGGTCAATTTGAACATTTACTAAATCGATCATAGTCAGTTCGGTGGCAGTTTAGCCGAGAAACCGACCCCAACCGACCGATGCTCACCCCTTCAATAGGTTATTGGGTTGATTTATAAATCAAGATCATTTTTACAATTTCCAATAAAAATGGAACAATTTTGAAAAAACCCACCATTTTCATTTTAGGCCAATGCAATTGAATTTCCCAACGACGTCGTTTTTTAAGTATCAGGTTCAAGGGCTGTCTCTTCCTCTGGCAAAGGATCAGAACCCATGTCTTCGTTATCCATCACTCCTCAAATTTCACCGAAGAGGTTTCAAAATCTCCTAAACCCTAATTTTCTTTTTCATCTTTTCTTGATTTTTTTTTCCTCTCTAATGGCCGTCCATTTCGCCATTGAAGTTCTTCACCAGTGGAAACTAGAAGACCATACACAATCTGCGCGTCTCAGAGATCTTCAATTCCAATCGGACGCCTCTCTAAATTCAGAAATTTCACCTCGCGGTTCCGTATGAGTATTGATAATGGAAGCTCTAAACGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGCCGCCGGGAATCTAATCTCATGCTGAGTAAGTTAGGTCTTAGACTTTGGAATTTTGGGTGAAGTTTTGAAGTTATTCGTTGGATAATTCTCTTGTGGTGATGAATTTTTTTGGAAATTTTCAGATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGTATGTTCTTTACAACTGTTTCTTATACACTAAACTGCTACTTGCATTCTTGAAACAACATTATTTATTTTGGTAGTTGGTTTTTGAAAATCAATGCAGCATTTTATTTAGGGGCTGCTTGGGTTGTAATGAATCGAGTTTGTAATGTAATGGGTCCAAAACTCATGTTTGGATTCAGCATTTCGATTCAGCATTTCGGCCCGATTTGTAATACCAAACTTATGTCCTTCCCCGGAGTTTTCAATCCAACCCTCTTTTCCACAGCTTTCATGCTTTACGATTCCATTTCGCATCTCAATGCTTCTCATATTACTAGTAATTCTGATTTCATTCTAGCTCTCCAAACATTCCCTTCAAAGGATTCTGGTCCCTACAAGTGATATACTGATTCTGGTCCCTACAAGTGATATTTTGAAAGTATAGGGACCGAAATAAACATTTTGAAAATATCAAAATGTATCAAGTATAAGGACCAAACTAACATTTTAAACCTTCTGTTTAGTTCAACAATGGTGGTGCAAACCACTTTTTTTTGTTCATGGTATATGTGTAAACCAATTGAACTATGTGTTAAGTTATTAACATTTGATCGATCAATTAGGACTAAAAGTAAACCATGATGAAGTAAACTATAATTCAATTACTCAACATCAAGATTGAATCTTTTGATTTTTGAAAAAATGTACAGTTTTGTGGAATTTTTTTTTCTACACTCTGAAGTAGAGCATCTCTAGACTTCAAATTAACATAAGACCCAATGAATAGAAAAGCAAAATAAATACTGATACCATTCAAAATGAATACTGATTTGGAACTATTCTATGTCATCGTACTGCCAGTAGTTTCTTTGTCTTTTTATTCCTTTCTGTCTCTTTTTGTTGCATTCAACACATGATAGAGTAACAGTCACAGCCATGTCCAATGTACTTATGGTTTCATTACTTTTTTCTGAAACAACATAGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATTGTGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCCGGTCGACATTGGCAACAGGTATGTAACTCACTATAGGACACTTGATTTGATGAAATGAACTGGTGGACTTTGCATCTTTTGCTAAGAGGATTGATGATAGCAATGAGTGAAACTTTGCAGGATATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATAACCGCTCCTCTCGGTCTTCACGAACAACTTGTGGTAGGTATTTCTAAAGTATATTTTTTTCTAGTATAGAATAGAAAAAGGCTGAAAATATCCATTGACATGTCGATATGTCCATAAATCCAAAGGTTCAACATCCATATTAAATATCCATGGATTCCAACATCTTTTATAAATGCTTGTAAAACAAAACAAAAAATTGTCATTGTTTAGTCTAATATGAATAGGAATGTTAATAACAATATCCATAAGGAAAACAATTTAATTGTTAATCTAAATAAATTTTATAAATTTCGTGACTTCCATAAATATCCATCAGTATCAATATTTCATCAAAATATCTGTCGATATTTCCGTAAAATTGAAATCTCTCAATTGGAGAATGAATGTCATAAATGATAAAAGAAAAGAACTATTTTTTAGTATTGAACTATGAGGTGGTTTTATTGGCAGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACAGGCAAATGTCGATTTAGTTAG

mRNA sequence

ATGAACACAGTGAACATTCCCTTTTCTTCACCAGTGGAAACTAGAAGACCATACACAATCTGCGCGTCTCAGAGATCTTCAATTCCAATCGGACGCCTCTCTAAATTCAGAAATTTCACCTCGCGGTTCCGTATGAGTATTGATAATGGAAGCTCTAAACGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGCCGCCGGGAATCTAATCTCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATTGTGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCCGGTCGACATTGGCAACAGGATATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATAACCGCTCCTCTCGGTCTTCACGAACAACTTGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACAGGCAAATGTCGATTTAGTTAG

Coding sequence (CDS)

ATGAACACAGTGAACATTCCCTTTTCTTCACCAGTGGAAACTAGAAGACCATACACAATCTGCGCGTCTCAGAGATCTTCAATTCCAATCGGACGCCTCTCTAAATTCAGAAATTTCACCTCGCGGTTCCGTATGAGTATTGATAATGGAAGCTCTAAACGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGCCGCCGGGAATCTAATCTCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATTGTGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCCGGTCGACATTGGCAACAGGATATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATAACCGCTCCTCTCGGTCTTCACGAACAACTTGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACAGGCAAATGTCGATTTAGTTAG

Protein sequence

MNTVNIPFSSPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRFS
Homology
[Close]
BLAST of PI0024116 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.1e-63
Identity = 111/143 (77.62%), Postives = 125/143 (87.41%), Query Frame = 0 

Query: 55  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVE 114
           IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+LCV+
Sbjct: 78  IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLCVQ 137

Query: 115 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 174
           QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDRI 
Sbjct: 138 QGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDRIQ 197

Query: 175 HCLSHAAGVADECEVCAGTGKCR 198
           HCLSH  G ADEC VCAGT KC+
Sbjct: 198 HCLSHVEGDADECLVCAGTNKCK 220

[Close]
BLAST of PI0024116 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.3e-16
Identity = 42/123 (34.15%), Postives = 64/123 (52.03%), Query Frame = 0 

Query: 52  SKRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTL 111
           +K +   DAV+ V HGSR +E       F+   +     PI E   +ELA P+I   F  
Sbjct: 6   TKEVDYMDAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEA 65

Query: 112 CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVND 171
           C+EQGA RI + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +
Sbjct: 66  CIEQGATRIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVE 125

Query: 172 RIN 175
           RIN
Sbjct: 126 RIN 128

[Close]
BLAST of PI0024116 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.6e-13
Identity = 41/141 (29.08%), Postives = 67/141 (47.52%), Query Frame = 0 

Query: 59  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAK 118
           +A+++V HGSR   S  ++ E     + +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 119 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKDH-------------------PGISY 175
           ++IV+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]
BLAST of PI0024116 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-12
Identity = 43/138 (31.16%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 59  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAK 118
           +A+++V HGSR   S  +L +     +++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 119 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDH------------------------PGISYI 173
           RIIV P FL  G H  +DIP L      +H                          +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]
BLAST of PI0024116 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-11
Identity = 40/138 (28.99%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 59  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAK 118
           +A++++ HGSR   S  ++ +     ++K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 119 RIIVSPFFLFPGRHWQQDIPSLTA--EAAKDH----------------------PGISYI 173
           +II+ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]
BLAST of PI0024116 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 4.6e-101
Identity = 181/191 (94.76%), Postives = 189/191 (98.95%), Query Frame = 0 

Query: 9   SSPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGS 68
           SSPVETRR ++ICASQRSSIP+GRLSKFRNFTSRFRMSIDNGSS RIGAGDAVIIVDHGS
Sbjct: 15  SSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNRIGAGDAVIIVDHGS 74

Query: 69  RRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLF 128
           RRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFTLCVEQGAKRIIVSPFFLF
Sbjct: 75  RRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAKRIIVSPFFLF 134

Query: 129 PGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECE 188
           PGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN+CLSHAAGV+DECE
Sbjct: 135 PGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINYCLSHAAGVSDECE 194

Query: 189 VCAGTGKCRFS 200
           VCAGTGKCRF+
Sbjct: 195 VCAGTGKCRFN 205

[Close]
BLAST of PI0024116 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.3e-100
Identity = 182/190 (95.79%), Postives = 184/190 (96.84%), Query Frame = 0 

Query: 10  SPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGSR 69
           SPVETR PYTICA QRS IPIGRLSKFRNFTSR RMSIDNGSS RIGAGDAVIIVDHGSR
Sbjct: 16  SPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSR 75

Query: 70  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLFP 129
           RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT CVEQGAKRIIVSPFFLFP
Sbjct: 76  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFP 135

Query: 130 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEV 189
           GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVADECEV
Sbjct: 136 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEV 195

Query: 190 CAGTGKCRFS 200
           CAGTGKCRF+
Sbjct: 196 CAGTGKCRFN 205

[Close]
BLAST of PI0024116 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.3e-100
Identity = 182/190 (95.79%), Postives = 184/190 (96.84%), Query Frame = 0 

Query: 10  SPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGSR 69
           SPVETR PYTICASQRS IPIGRLSKFRNFTSR RMSIDNGSS RIGAGDAVIIVDHGSR
Sbjct: 16  SPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSR 75

Query: 70  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLFP 129
           RRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT CVEQGAKRIIVSPFFLFP
Sbjct: 76  RRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFP 135

Query: 130 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEV 189
           GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVADECEV
Sbjct: 136 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEV 195

Query: 190 CAGTGKCRFS 200
           CAGTGKCRF+
Sbjct: 196 CAGTGKCRFN 205

[Close]
BLAST of PI0024116 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.7e-92
Identity = 169/198 (85.35%), Postives = 180/198 (90.91%), Query Frame = 0 

Query: 4   VNIPFSSPVETRRPYTICASQRSS--IPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAV 63
           + +   S  ETR+ YTICASQ+SS  I IGR S  RNF+SRFRMS+DNG S RIGAGDAV
Sbjct: 10  ITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGSNRIGAGDAV 69

Query: 64  IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRII 123
           IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF  CVEQGAKRI+
Sbjct: 70  IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIV 129

Query: 124 VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAA 183
           VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRI+HCLSHAA
Sbjct: 130 VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAA 189

Query: 184 GVADECEVCAGTGKCRFS 200
           GV +ECEVCAGTGKC+F+
Sbjct: 190 GVVEECEVCAGTGKCKFN 207

[Close]
BLAST of PI0024116 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.6e-92
Identity = 167/198 (84.34%), Postives = 180/198 (90.91%), Query Frame = 0 

Query: 4   VNIPFSSPVETRRPYTICASQRSS--IPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAV 63
           + +   S  ETR+ YTICASQ+SS  I +GR S  RNF+SRFRMS+DNG + RIGAGDAV
Sbjct: 10  ITVKSPSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGGADRIGAGDAV 69

Query: 64  IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRII 123
           IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF  CVEQGAKRI+
Sbjct: 70  IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIV 129

Query: 124 VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAA 183
           VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRI+HCLSHAA
Sbjct: 130 VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAA 189

Query: 184 GVADECEVCAGTGKCRFS 200
           GV +ECEVCAGTGKC+F+
Sbjct: 190 GVVEECEVCAGTGKCKFN 207

[Close]
BLAST of PI0024116 vs. NCBI nr
Match: XP_031742215.1 (sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hypothetical protein Csa_008200 [Cucumis sativus])

HSP 1 Score: 377.1 bits (967), Expect = 9.4e-101
Identity = 181/191 (94.76%), Postives = 189/191 (98.95%), Query Frame = 0 

Query: 9   SSPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGS 68
           SSPVETRR ++ICASQRSSIP+GRLSKFRNFTSRFRMSIDNGSS RIGAGDAVIIVDHGS
Sbjct: 15  SSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNRIGAGDAVIIVDHGS 74

Query: 69  RRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLF 128
           RRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFTLCVEQGAKRIIVSPFFLF
Sbjct: 75  RRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAKRIIVSPFFLF 134

Query: 129 PGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECE 188
           PGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN+CLSHAAGV+DECE
Sbjct: 135 PGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINYCLSHAAGVSDECE 194

Query: 189 VCAGTGKCRFS 200
           VCAGTGKCRF+
Sbjct: 195 VCAGTGKCRFN 205

[Close]
BLAST of PI0024116 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 375.6 bits (963), Expect = 2.7e-100
Identity = 182/190 (95.79%), Postives = 184/190 (96.84%), Query Frame = 0 

Query: 10  SPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGSR 69
           SPVETR PYTICA QRS IPIGRLSKFRNFTSR RMSIDNGSS RIGAGDAVIIVDHGSR
Sbjct: 16  SPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSR 75

Query: 70  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLFP 129
           RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT CVEQGAKRIIVSPFFLFP
Sbjct: 76  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFP 135

Query: 130 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEV 189
           GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVADECEV
Sbjct: 136 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEV 195

Query: 190 CAGTGKCRFS 200
           CAGTGKCRF+
Sbjct: 196 CAGTGKCRFN 205

[Close]
BLAST of PI0024116 vs. NCBI nr
Match: XP_008458790.1 (PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo])

HSP 1 Score: 375.6 bits (963), Expect = 2.7e-100
Identity = 182/190 (95.79%), Postives = 184/190 (96.84%), Query Frame = 0 

Query: 10  SPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAVIIVDHGSR 69
           SPVETR PYTICASQRS IPIGRLSKFRNFTSR RMSIDNGSS RIGAGDAVIIVDHGSR
Sbjct: 16  SPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSR 75

Query: 70  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRIIVSPFFLFP 129
           RRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT CVEQGAKRIIVSPFFLFP
Sbjct: 76  RRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFP 135

Query: 130 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEV 189
           GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVADECEV
Sbjct: 136 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEV 195

Query: 190 CAGTGKCRFS 200
           CAGTGKCRF+
Sbjct: 196 CAGTGKCRFN 205

[Close]
BLAST of PI0024116 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 360.9 bits (925), Expect = 7.0e-96
Identity = 177/200 (88.50%), Postives = 187/200 (93.50%), Query Frame = 0 

Query: 4   VNIPFSSPVETRRPYTICASQRSSIPIGRLSKFRNFTSRFRMSIDNGSSK----RIGAGD 63
           +++  SS VETRR YTICASQ+S I I RLS+FRNFTSRFRMS+DNGSS     RIGAGD
Sbjct: 47  ISLKSSSAVETRRKYTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSSNRSGDRIGAGD 106

Query: 64  AVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKR 123
           AVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF LCVEQGAKR
Sbjct: 107 AVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFALCVEQGAKR 166

Query: 124 IIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSH 183
           IIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRI+HCLSH
Sbjct: 167 IIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRISHCLSH 226

Query: 184 AAGVADECEVCAGTGKCRFS 200
           AAGVA+ECEVCAGTGKCRF+
Sbjct: 227 AAGVAEECEVCAGTGKCRFN 246

[Close]
BLAST of PI0024116 vs. NCBI nr
Match: XP_022993758.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 348.6 bits (893), Expect = 3.6e-92
Identity = 169/198 (85.35%), Postives = 180/198 (90.91%), Query Frame = 0 

Query: 4   VNIPFSSPVETRRPYTICASQRSS--IPIGRLSKFRNFTSRFRMSIDNGSSKRIGAGDAV 63
           + +   S  ETR+ YTICASQ+SS  I IGR S  RNF+SRFRMS+DNG S RIGAGDAV
Sbjct: 10  ITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGSNRIGAGDAV 69

Query: 64  IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVEQGAKRII 123
           IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF  CVEQGAKRI+
Sbjct: 70  IIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIV 129

Query: 124 VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAA 183
           VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRI+HCLSHAA
Sbjct: 130 VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAA 189

Query: 184 GVADECEVCAGTGKCRFS 200
           GV +ECEVCAGTGKC+F+
Sbjct: 190 GVVEECEVCAGTGKCKFN 207

[Close]
BLAST of PI0024116 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 243.4 bits (620), Expect = 1.5e-64
Identity = 111/143 (77.62%), Postives = 125/143 (87.41%), Query Frame = 0 

Query: 55  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTLCVE 114
           IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+LCV+
Sbjct: 78  IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLCVQ 137

Query: 115 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 174
           QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDRI 
Sbjct: 138 QGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDRIQ 197

Query: 175 HCLSHAAGVADECEVCAGTGKCR 198
           HCLSH  G ADEC VCAGT KC+
Sbjct: 198 HCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:
BLAST of PI0024116 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JH72.1e-6377.62Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618173.3e-1634.15Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
P618192.6e-1329.08Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
Q583801.0e-1231.16Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
A6UWT51.9e-1128.99Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
BLAST of PI0024116 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KP174.6e-10194.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1[more]
A0A5D3BY551.3e-10095.79Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C8S91.3e-10095.79sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1K3841.7e-9285.35sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1FHT56.6e-9284.34sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
BLAST of PI0024116 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031742215.19.4e-10194.76sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hyp... [more]
KAA0037556.12.7e-10095.79sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro... [more]
XP_008458790.12.7e-10095.79PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo][more]
XP_038890885.17.0e-9688.50sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida][more]
XP_022993758.13.6e-9285.35sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima][more]
BLAST of PI0024116 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50170.11.5e-6477.62sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 66..170
e-value: 1.5E-29
score: 102.4
NoneNo IPR availableGENE3D3.40.50.1400coord: 57..180
e-value: 8.3E-34
score: 118.5
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 52..196
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 52..196
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 60..160
e-value: 4.38135E-38
score: 125.377
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 59..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0024116.1PI0024116.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019354 siroheme biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0042802 identical protein binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051266 sirohydrochlorin ferrochelatase activity
molecular_function GO:0016829 lyase activity