PI0024010 (gene) Melon (PI 482460) v1

Overview
NamePI0024010
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionVinorine synthase-like
Locationchr03: 370402 .. 371779 (+)
RNA-Seq ExpressionPI0024010
SyntenyPI0024010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCGAAGTTGACATTATCTCCGAAGAGTTAATCAAACCTTCTACCCCAACTCCTCCCCATCTTCGCCATTACTCCCTCTCTTTCATTGATCAACTCACCGTTCACATTTACGCACCCGTCCTCTACTTCTACCAATCCACCGACAACGGCCCAGGTGGTGAAGATCTCGATTTTGCTCTAGCCTTCAAAAACCGTCTTCGGAGCACCCTCCCGGACGTCCTAGCCCACTACCCGCCTCTCGCTGGCCGTCCAAATTACGCTTCTTCCTTCATCGACTGCAACGATACCGGCGTCCCCTTCCGAGAAGCGCGAGTCAACTCGCAACTCGCCGACGTGATCCAATTCGCGCAGCCGGACGATCTCAACCGGCTATTTCCGGTCGAGTTGGACCGGTTCAACGAAGAATTAATGGCGGTTCAGTTCACGGAATTCGCGTGCGGGGGAGTTGCGGTTGCGTCGTGCATTTCGCACAAGATAGCCGACGGGATGGCGCTTTTTTCGTTGAACAACAACTGGGCCGCCATGGCTCGTGGCGTGAAAGGCGTTTTCAAGCCCCACATGGAAGGCGCCAAAATCTTCCCACCCAAAACGATGTCGTACGATGCGGCGATGATGATCGCGAGAAACAGAGTCTCAAGAAGATTTGTTTTCAAGCAATCGAAACTGGAAGCCATTAGAGCCAAATACACAGAAAATCAAGCTATGATTAACCTGAATCGACCTTCACGAATTGAATCCTTAACAGCTTTTGTTTACAGCCGATTCCTCGCAGCCTTTAAACACGATTCTGCAATTCGAAACAAGTCATTTTTAGTAAACTACACAGTAAATCTCCGTCCAAAAATGAATCCACCACTTCCACATGACGCATTCGGGAATTACTATTTCAACGTCATGGTTTTCCCTTCGCCGGAAACGCTCAACGACGATGAAAATTGCTACGGTTTGGTGAAGCAACTGAGAGAAGAAAAGAACAAAATAGATGGAGAAATGGCGAAGAAATTTCTGAATGAAGACAAGGATCTGATGGAAACTGTGAAGGAAGTTGCTTCGAAAGTGATTAATGGAGAGTTAATTTCGTGTGCTTTCAGTAGTGTCTGTAGATTACCGCTTTATGATGTGGATTTCGGATGGGGAAGGCCTGTTTGGGTTACTTTTCCGGCGCTGTGGTTCAAGAATCTGGTGGCGTTTTTGGACTCGAAAGATGGAGAAGGCATTGATGCAATCGTTCACTTGGAAGAACAACACATGAATAAGCTTGAAGGTGATGAAGTGTTCATGAAATATGCTACTCCTATTCCTACCCCTTCTTCCCCACTCGCCTGAAAAATCAAACCCCTCTAGCTATTTGTTACTATTTTCTTTGTGCTTT

mRNA sequence

ATGAAGCTCGAAGTTGACATTATCTCCGAAGAGTTAATCAAACCTTCTACCCCAACTCCTCCCCATCTTCGCCATTACTCCCTCTCTTTCATTGATCAACTCACCGTTCACATTTACGCACCCGTCCTCTACTTCTACCAATCCACCGACAACGGCCCAGGTGGTGAAGATCTCGATTTTGCTCTAGCCTTCAAAAACCGTCTTCGGAGCACCCTCCCGGACGTCCTAGCCCACTACCCGCCTCTCGCTGGCCGTCCAAATTACGCTTCTTCCTTCATCGACTGCAACGATACCGGCGTCCCCTTCCGAGAAGCGCGAGTCAACTCGCAACTCGCCGACGTGATCCAATTCGCGCAGCCGGACGATCTCAACCGGCTATTTCCGGTCGAGTTGGACCGGTTCAACGAAGAATTAATGGCGGTTCAGTTCACGGAATTCGCGTGCGGGGGAGTTGCGGTTGCGTCGTGCATTTCGCACAAGATAGCCGACGGGATGGCGCTTTTTTCGTTGAACAACAACTGGGCCGCCATGGCTCGTGGCGTGAAAGGCGTTTTCAAGCCCCACATGGAAGGCGCCAAAATCTTCCCACCCAAAACGATGTCGTACGATGCGGCGATGATGATCGCGAGAAACAGAGTCTCAAGAAGATTTGTTTTCAAGCAATCGAAACTGGAAGCCATTAGAGCCAAATACACAGAAAATCAAGCTATGATTAACCTGAATCGACCTTCACGAATTGAATCCTTAACAGCTTTTGTTTACAGCCGATTCCTCGCAGCCTTTAAACACGATTCTGCAATTCGAAACAAGTCATTTTTAGTAAACTACACAGTAAATCTCCGTCCAAAAATGAATCCACCACTTCCACATGACGCATTCGGGAATTACTATTTCAACGTCATGGTTTTCCCTTCGCCGGAAACGCTCAACGACGATGAAAATTGCTACGGTTTGGTGAAGCAACTGAGAGAAGAAAAGAACAAAATAGATGGAGAAATGGCGAAGAAATTTCTGAATGAAGACAAGGATCTGATGGAAACTGTGAAGGAAGTTGCTTCGAAAGTGATTAATGGAGAGTTAATTTCGTGTGCTTTCAGTAGTGTCTGTAGATTACCGCTTTATGATGTGGATTTCGGATGGGGAAGGCCTGTTTGGGTTACTTTTCCGGCGCTGTGGTTCAAGAATCTGGTGGCGTTTTTGGACTCGAAAGATGGAGAAGGCATTGATGCAATCGTTCACTTGGAAGAACAACACATGAATAAGCTTGAAGGTGATGAAGTGTTCATGAAATATGCTACTCCTATTCCTACCCCTTCTTCCCCACTCGCCTGAAAAATCAAACCCCTCTAGCTATTTGTTACTATTTTCTTTGTGCTTT

Coding sequence (CDS)

ATGAAGCTCGAAGTTGACATTATCTCCGAAGAGTTAATCAAACCTTCTACCCCAACTCCTCCCCATCTTCGCCATTACTCCCTCTCTTTCATTGATCAACTCACCGTTCACATTTACGCACCCGTCCTCTACTTCTACCAATCCACCGACAACGGCCCAGGTGGTGAAGATCTCGATTTTGCTCTAGCCTTCAAAAACCGTCTTCGGAGCACCCTCCCGGACGTCCTAGCCCACTACCCGCCTCTCGCTGGCCGTCCAAATTACGCTTCTTCCTTCATCGACTGCAACGATACCGGCGTCCCCTTCCGAGAAGCGCGAGTCAACTCGCAACTCGCCGACGTGATCCAATTCGCGCAGCCGGACGATCTCAACCGGCTATTTCCGGTCGAGTTGGACCGGTTCAACGAAGAATTAATGGCGGTTCAGTTCACGGAATTCGCGTGCGGGGGAGTTGCGGTTGCGTCGTGCATTTCGCACAAGATAGCCGACGGGATGGCGCTTTTTTCGTTGAACAACAACTGGGCCGCCATGGCTCGTGGCGTGAAAGGCGTTTTCAAGCCCCACATGGAAGGCGCCAAAATCTTCCCACCCAAAACGATGTCGTACGATGCGGCGATGATGATCGCGAGAAACAGAGTCTCAAGAAGATTTGTTTTCAAGCAATCGAAACTGGAAGCCATTAGAGCCAAATACACAGAAAATCAAGCTATGATTAACCTGAATCGACCTTCACGAATTGAATCCTTAACAGCTTTTGTTTACAGCCGATTCCTCGCAGCCTTTAAACACGATTCTGCAATTCGAAACAAGTCATTTTTAGTAAACTACACAGTAAATCTCCGTCCAAAAATGAATCCACCACTTCCACATGACGCATTCGGGAATTACTATTTCAACGTCATGGTTTTCCCTTCGCCGGAAACGCTCAACGACGATGAAAATTGCTACGGTTTGGTGAAGCAACTGAGAGAAGAAAAGAACAAAATAGATGGAGAAATGGCGAAGAAATTTCTGAATGAAGACAAGGATCTGATGGAAACTGTGAAGGAAGTTGCTTCGAAAGTGATTAATGGAGAGTTAATTTCGTGTGCTTTCAGTAGTGTCTGTAGATTACCGCTTTATGATGTGGATTTCGGATGGGGAAGGCCTGTTTGGGTTACTTTTCCGGCGCTGTGGTTCAAGAATCTGGTGGCGTTTTTGGACTCGAAAGATGGAGAAGGCATTGATGCAATCGTTCACTTGGAAGAACAACACATGAATAAGCTTGAAGGTGATGAAGTGTTCATGAAATATGCTACTCCTATTCCTACCCCTTCTTCCCCACTCGCCTGA

Protein sequence

MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDFALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARGVKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPHDAFGNYYFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVINGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQHMNKLEGDEVFMKYATPIPTPSSPLA
Homology
BLAST of PI0024010 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.5e-61
Identity = 154/433 (35.57%), Postives = 238/433 (54.97%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           M  ++ I+SEELI+PS+PTP  L+ + LS +DQ+ +  + P++ FY +  +     +LD 
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLD----SNLDR 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           A   +N L+ +L  VL  + PLAGR N  SS +DCND+GVPF EARV+SQL++ I+    
Sbjct: 61  AQRSEN-LKRSLSTVLTQFYPLAGRININSS-VDCNDSGVPFLEARVHSQLSEAIKNVAI 120

Query: 121 DDLNRLFPVELDRFNEEL-------MAVQFTEFACGGVAVASCISHKIADGMALFSLNNN 180
           D+LN+  P +     EE        +AV+ + F CGG A+  CISHKIAD ++L +  N+
Sbjct: 121 DELNQYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNS 180

Query: 181 WAAMARGVKGVFKPHME-GAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYT 240
           W A  +    + +P+ + G+  FPP         +   N V +RFVF + KLEA++A+  
Sbjct: 181 WTATCQEETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLA 240

Query: 241 ENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPHDA 300
            +     +   SR++ + A ++ +F+   +     +NK  +    VNLR +MNPP P  A
Sbjct: 241 SSAT--EVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNK-LVAAQAVNLRSRMNPPFPQSA 300

Query: 301 FGNYYFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVA 360
            GN     M +   E   +D++   LV  L+    KID E         K+L + V  + 
Sbjct: 301 MGN--IATMAYAVAE---EDKDFSDLVGPLKTSLAKIDDEHV-------KELQKGVTYLD 360

Query: 361 SKVINGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIV 420
            +    EL S  FSS CRL  YD+DFGWG+PV V    +  KNLV  +D+++ +G++A +
Sbjct: 361 YEAEPQELFS--FSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWI 410

Query: 421 HLEEQHMNKLEGD 426
            + E  M+ L  D
Sbjct: 421 SMAEDEMSMLSSD 410

BLAST of PI0024010 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 2.4e-54
Identity = 149/433 (34.41%), Postives = 233/433 (53.81%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           M  +++ +SEELI PS+PTP  L+ Y +S +DQL +  + P + FY +    P   +LD 
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPN----PLDSNLDP 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQ-FAQ 120
           A      L+ +L  VL H+ PLAGR N  SS +DCND+GVPF EARV +QL+  IQ   +
Sbjct: 61  AQT-SQHLKQSLSKVLTHFYPLAGRINVNSS-VDCNDSGVPFVEARVQAQLSQAIQNVVE 120

Query: 121 PDDLNR-----LFPVELDRFNEEL-MAVQFTEFACGGVAVASCISHKIADGMALFSLNNN 180
            + L++      +P      NE++ +AV+ + F CGG A+   +SHKIAD ++L +  N 
Sbjct: 121 LEKLDQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNA 180

Query: 181 WAAMARGVKGVFKPHME-GAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYT 240
           W A  RG   +  P+ +  A+ FPP   +    ++   N V +RFVF + K+ A+RA+ +
Sbjct: 181 WTATCRGETEIVLPNFDLAARHFPPVDNTPSPELVPDENVVMKRFVFDKEKIGALRAQAS 240

Query: 241 ENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPHDA 300
                 N    SR++ + A+++   +   +     +NK F+V   VNLR +MNPPLPH A
Sbjct: 241 SASEEKNF---SRVQLVVAYIWKHVIDVTRAKYGAKNK-FVVVQAVNLRSRMNPPLPHYA 300

Query: 301 FGNYYFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVA 360
            GN     ++F + +    D++   L+  LR    K + +   + L     L E   +  
Sbjct: 301 MGN--IATLLFAAVDA-EWDKDFPDLIGPLRTSLEKTEDDHNHELLKGMTCLYELEPQ-- 360

Query: 361 SKVINGELISCAFSSVCRLPLYDVDFGWGRPV---WVTFPALWFKNLVAFLDSKDGEGID 420
                 EL+S  F+S CRL  YD+DFGWG+P+     TFP    +N    +D++ G+G++
Sbjct: 361 ------ELLS--FTSWCRLGFYDLDFGWGKPLSACTTTFPK---RNAALLMDTRSGDGVE 407

Query: 421 AIVHLEEQHMNKL 423
           A + + E  M  L
Sbjct: 421 AWLPMAEDEMAML 407

BLAST of PI0024010 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.0e-49
Identity = 143/452 (31.64%), Postives = 237/452 (52.43%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           + +E + +S+ LIKPS+PTP  L  Y+LS+ DQ        V +FY++    P G ++  
Sbjct: 6   ISVETETLSKTLIKPSSPTPQSLSRYNLSYNDQNIYQTCVSVGFFYEN----PDGIEIS- 65

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQF--- 120
               + +L+++L   L  Y P AG+    + +I CND G+ F E R+  ++ D++++   
Sbjct: 66  --TIREQLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVEVRIRCRMNDILKYELR 125

Query: 121 AQPDDLNRLFPVELDRFNEELMA-VQFTEFACGGVAVASCISHKIADGMALFSLNNNWAA 180
           +   DL  + P  +   +E+  A VQ + F CGG+AVA  ISHK+ADG  + S   +WAA
Sbjct: 126 SYARDL--VLPKRVTVGSEDTTAIVQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAA 185

Query: 181 MARGVKG---VFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTE 240
            A  +     V  P +    IFP +          ++N V + F+F    +E +++K  E
Sbjct: 186 SACYLSSSHHVPTPLLVSDSIFPRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLKSKAVE 245

Query: 241 NQAMINLNRPSRIESLTAFVYSRFL-----AAFKHDSAIRNKSFLVNYTVNLRPKMNPPL 300
                 + +P+R+E LTAF+ SR       +A K+++  ++  F V   +NLRP +   L
Sbjct: 246 ----FGIEKPTRVEVLTAFL-SRCATVAGKSAAKNNNCGQSLPFPVLQAINLRPILE--L 305

Query: 301 PHDAFGN---YYFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLM 360
           P ++ GN    YF+       + LN+ E    ++ +LR+EK KI   ++++ L     + 
Sbjct: 306 PQNSVGNLVSIYFS-RTIKENDYLNEKEYTKLVINELRKEKQKIK-NLSREKLTYVAQME 365

Query: 361 ETVKEVASKVINGELISCAF--SSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSK 420
           E VK +    I+  L   A+   S CR P YDVDFGWG+P+WV     + KN V  +D  
Sbjct: 366 EFVKSLKEFDISNFLDIDAYLSDSWCRFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMDYP 425

Query: 421 DGE--GIDAIVHLEEQHMNKLEGDEVFMKYAT 434
            G+  GI+AIV  E++ M+  E +E  +++ +
Sbjct: 426 FGDDYGIEAIVSFEQEKMSAFEKNEQLLQFVS 438

BLAST of PI0024010 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.3e-47
Identity = 143/459 (31.15%), Postives = 234/459 (50.98%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDFAL 62
           +E + +S+ LIKPS+PTP  L HY+LS+ DQ     Y    +FY    + P G ++    
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFY----SNPDGHEIS--- 66

Query: 63  AFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDD 122
             + +L+++L   L  Y P AG+    + +I CND G+ F + R++ ++ D++   +P+ 
Sbjct: 67  TIREQLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVDVRIHCRMNDIL---KPE- 126

Query: 123 LNRLFPVELDRFNEELMA-------VQFTEFACGGVAVASCISHKIADGMALFSLNNNWA 182
             R +  EL R N   +        VQ + F CGGVAVA  ISHK+AD   + S   +WA
Sbjct: 127 -LRSYASELIRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWA 186

Query: 183 AMARGVKG---VFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYT 242
           A    +     V  P +    IFP +          + N V +RF+F    +E +++K  
Sbjct: 187 ASTCDLSSSHDVSTPVLVSDSIFPRQDNIICGQFPASPNCVRKRFLFSPEAIERLKSKAI 246

Query: 243 ENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKS------FLVNYTVNLRPKMNP 302
           E      + +P+R+E LTAF+      A K  SA +N +      F V   VNLRP +  
Sbjct: 247 E----FGIEKPTRVEVLTAFLCRCATVAGK--SAAKNNNCGQSLPFAVIQAVNLRPLLE- 306

Query: 303 PLPHDAFGN---YYFNVMVFPSPETLNDDENCYGLV-KQLREEKNKIDGEMAKK--FLNE 362
            LP ++ GN    YF+ +     +T+N ++    LV  +LR+ K+K+     +K  ++  
Sbjct: 307 -LPKNSVGNLISIYFSTI--KENDTVNIEQEFTKLVIGELRKAKDKLKNLSQEKLNYVAR 366

Query: 363 DKDLMETVKEVASK----VINGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNL 422
            +D    +KE+       + N ++ +  FSS CR P YD+DFG G+P+WV      FKN 
Sbjct: 367 MQDFANCLKELDISSFFDMENVDIDAYLFSSWCRFPFYDIDFGLGKPIWVCMFQPHFKNC 426

Query: 423 VAFLDSKDGE--GIDAIVHLEEQHMNKLEGDEVFMKYAT 434
           +  +D   G+  GI+A++ LE++ M   E +E+ + +A+
Sbjct: 427 IILMDYPFGDDYGIEALITLEQEKMPAFENNELLLSFAS 442

BLAST of PI0024010 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 8.1e-47
Identity = 133/437 (30.43%), Postives = 223/437 (51.03%), Query Frame = 0

Query: 5   VDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDFALAF 64
           V+I+S+  IKPS+PTP  LR Y+LS IDQ        + +FY      P  E        
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY---TYNPSHESSKIGDLL 65

Query: 65  KNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDDLN 124
           KN L  T    L  Y   AGR    + +IDCND GV F E R++ ++ D+++  +    +
Sbjct: 66  KNSLSKT----LVSYYQFAGR-LIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATD 125

Query: 125 RLFPVELDRFNEE-LMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARGVKG 184
            + P  +   +E+ L+ VQ + F CGG+A+    SHK++DG++      +WA+ +  +  
Sbjct: 126 LVLPTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWAS-STSLST 185

Query: 185 VFKPH--MEGAKIFPPK--TMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMIN 244
             KP   +    IFPP+   +  + +++  +  + +RFVF    +E +++K  E      
Sbjct: 186 FHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE----YG 245

Query: 245 LNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPHDAFGNYYFN 304
           + +PSR+E +TAF+         +    R K + +   VNLRP +   LP ++ GN    
Sbjct: 246 IQKPSRVEVVTAFL----CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGN---- 305

Query: 305 VMVFPSPETLND---DENCYGLVKQLREEKNK-IDGEMAKKFLNEDKDLMETVKEVASKV 364
             +F     +ND   D     L+ +LR  K K ++  ++K+ L  +  + E  K +    
Sbjct: 306 --IFSFYFCINDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQLN 365

Query: 365 INGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLE 424
           I+  L +   SS CR P+YD+DFGWG+P+ V+      K+L+  +DS +G+GI+A++ +E
Sbjct: 366 IS-SLDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALITME 416

Query: 425 EQHMNKLEGDEVFMKYA 433
           E+ M   E +E    +A
Sbjct: 426 EKKMAAFEKNEELRSFA 416

BLAST of PI0024010 vs. ExPASy TrEMBL
Match: A0A5A7UYQ7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001680 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.2e-244
Identity = 416/444 (93.69%), Postives = 425/444 (95.72%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQ+TVHIYAP LYFYQSTD G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAKIFPPK MSYD AM I RNRVSRRFVFKQSKLEAIRAKYTENQ  IN 
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRN-KSFLVNYTVNLRPKMNPPLPHDAFGNYYFN 300
           NRPSRIESLTAFVYSRFLAAFKHDSAIRN  SFLVNYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVINGE 360
           VM+FPSPETLNDDENCY LVKQLREE NKIDGEMAKKFLNEDKDLMETVKEVASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 LISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQHM 420
           +ISCAFSS+CR PLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEG+DAIVHLEE++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLEGDEVFMKYATPIPTPSSPLA 444
           NKLEGDEVFM YATP+PTPSSPLA
Sbjct: 421 NKLEGDEVFMNYATPLPTPSSPLA 444

BLAST of PI0024010 vs. ExPASy TrEMBL
Match: A0A0A0LQS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.2e-240
Identity = 410/445 (92.13%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQ+TVHIYAP LYFYQSTD G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYPPLAGRPNYASSFIDCNDTGVPFREA VNSQLADVIQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAKIFPPK MSYD+AM I RNRVSRRFVFKQSK+EAIR KYTENQ MIN 
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 241 N-RPSRIESLTAFVYSRFLAAFKHDSAIRN-KSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300
           N +PSRIESLTAFVYSRFLAAFKHDS IRN  SFLVNYTVNLRPKMNPPLPHDAFGNYYF
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKHDSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300

Query: 301 NVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVING 360
           NVM+FPSPETLNDDENCYGLVKQLREE NKIDGEMAKKFLNEDK+LM+TVKEVASKV++G
Sbjct: 301 NVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVVSG 360

Query: 361 ELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQH 420
           E++SCAFSS+CR PLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEE++
Sbjct: 361 EIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEERY 420

Query: 421 MNKLEGDEVFMKYATPIPTPSSPLA 444
           MNKLEGDEVFMKYATPIPTPSS +A
Sbjct: 421 MNKLEGDEVFMKYATPIPTPSSRVA 445

BLAST of PI0024010 vs. ExPASy TrEMBL
Match: A0A1S3BD01 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 3.8e-233
Identity = 399/425 (93.88%), Postives = 407/425 (95.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQ+TVHIYAP LYFYQSTD G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAKIFPPK MSYD AM I RNRVSRRFVFKQSKLEAIRAKYTENQ  IN 
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRN-KSFLVNYTVNLRPKMNPPLPHDAFGNYYFN 300
           NRPSRIESLTAFVYSRFLAAFKHDSAIRN  SFLVNYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVINGE 360
           VM+FPSPETLNDDENCY LVKQLREE NKIDGEMAKKFLNEDKDLMETVKEVASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 LISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQHM 420
           +ISCAFSS+CR PLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEG+DAIVHLEE++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLEG 425
           NKLEG
Sbjct: 421 NKLEG 425

BLAST of PI0024010 vs. ExPASy TrEMBL
Match: A0A6J1KPC8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.9e-187
Identity = 325/445 (73.03%), Postives = 375/445 (84.27%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLE+DIISE+LIKP+TPTPPHLRHY LSFIDQ+TVHIYAP LYFY+ T  G    D DF
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG----DPDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           A+ F+NRLRSTL  VL+HYPPLAGRPNY S+F+DCNDTG  FREARVN++LADV+++AQP
Sbjct: 61  AIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAK+FP KT  YD A  I RNRV+RRFVF+ SK+EAIR KY EN A    
Sbjct: 181 VKGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---A 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIR---NKSFLVNYTVNLRPKMNPPLPHDAFGNYY 300
           +RPSR+ESLTAF+Y RFLAA K  S+     +++ LVNYTVNLRPKM PPLP DAFGNYY
Sbjct: 241 HRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYY 300

Query: 301 FNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVIN 360
           FN M+FPSP+ LN D  C+GLV +LREE NK++ +  K  LNED+ LME+V+E+ASK + 
Sbjct: 301 FNAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVK 360

Query: 361 GELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQ 420
           GE+ISCAF+S+CR PLYDVDFGWGRPVWVTFPALWFKNLV  LDSK+  GIDAIVHLEE 
Sbjct: 361 GEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEP 420

Query: 421 HMNKLEGDEVFMKYATPIPT-PSSP 442
           HM +LE D++F++YATP PT P++P
Sbjct: 421 HMKRLEADQLFLEYATPTPTAPATP 437

BLAST of PI0024010 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.4e-187
Identity = 326/445 (73.26%), Postives = 375/445 (84.27%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLE+DIISE+LIKP+TPTPPHLR Y LSFIDQ+TVHIYAP LYFYQ    G    D DF
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAG----DPDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           A+ F+NRLRSTL  VL+HYPPLAGRPNY S+F+DCNDTG  FREARVN++LADV+++A+P
Sbjct: 61  AIDFRNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAK+FP KTM+YD A  I RNRV+RRFVF+ SK+EAIR KY EN A    
Sbjct: 181 VKGVFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---A 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIR---NKSFLVNYTVNLRPKMNPPLPHDAFGNYY 300
           +RPSR+ESLTAF+Y RFLAA K  S+ +   +++ LVNYTVNLR KM PPLP DAFGNYY
Sbjct: 241 HRPSRVESLTAFMYGRFLAALKDGSSSQVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYY 300

Query: 301 FNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVIN 360
           FN M+FPSP+ LN D  CYGLV +LREE NK++ +  K  LNED+ LME+V+E+ASK + 
Sbjct: 301 FNAMIFPSPDILN-DPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVK 360

Query: 361 GELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQ 420
           GE+ISCAF+S+CR PLYDVDFGWGRPVWVTFPALWFKNLV FLDSK+  GIDAIVHLEE 
Sbjct: 361 GEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEP 420

Query: 421 HMNKLEGDEVFMKYATPIPT-PSSP 442
           HM KLE D++F++YATP PT P +P
Sbjct: 421 HMKKLEADQLFLQYATPTPTAPPTP 437

BLAST of PI0024010 vs. NCBI nr
Match: KAA0061032.1 (vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 416/444 (93.69%), Postives = 425/444 (95.72%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQ+TVHIYAP LYFYQSTD G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAKIFPPK MSYD AM I RNRVSRRFVFKQSKLEAIRAKYTENQ  IN 
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRN-KSFLVNYTVNLRPKMNPPLPHDAFGNYYFN 300
           NRPSRIESLTAFVYSRFLAAFKHDSAIRN  SFLVNYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVINGE 360
           VM+FPSPETLNDDENCY LVKQLREE NKIDGEMAKKFLNEDKDLMETVKEVASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 LISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQHM 420
           +ISCAFSS+CR PLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEG+DAIVHLEE++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLEGDEVFMKYATPIPTPSSPLA 444
           NKLEGDEVFM YATP+PTPSSPLA
Sbjct: 421 NKLEGDEVFMNYATPLPTPSSPLA 444

BLAST of PI0024010 vs. NCBI nr
Match: XP_004143048.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical protein Csa_018752 [Cucumis sativus])

HSP 1 Score: 840.5 bits (2170), Expect = 6.7e-240
Identity = 410/445 (92.13%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQ+TVHIYAP LYFYQSTD G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYPPLAGRPNYASSFIDCNDTGVPFREA VNSQLADVIQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAKIFPPK MSYD+AM I RNRVSRRFVFKQSK+EAIR KYTENQ MIN 
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 241 N-RPSRIESLTAFVYSRFLAAFKHDSAIRN-KSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300
           N +PSRIESLTAFVYSRFLAAFKHDS IRN  SFLVNYTVNLRPKMNPPLPHDAFGNYYF
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKHDSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300

Query: 301 NVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVING 360
           NVM+FPSPETLNDDENCYGLVKQLREE NKIDGEMAKKFLNEDK+LM+TVKEVASKV++G
Sbjct: 301 NVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVVSG 360

Query: 361 ELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQH 420
           E++SCAFSS+CR PLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEE++
Sbjct: 361 EIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEERY 420

Query: 421 MNKLEGDEVFMKYATPIPTPSSPLA 444
           MNKLEGDEVFMKYATPIPTPSS +A
Sbjct: 421 MNKLEGDEVFMKYATPIPTPSSRVA 445

BLAST of PI0024010 vs. NCBI nr
Match: XP_008445555.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 817.0 bits (2109), Expect = 7.9e-233
Identity = 399/425 (93.88%), Postives = 407/425 (95.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQ+TVHIYAP LYFYQSTD G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD MALFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAKIFPPK MSYD AM I RNRVSRRFVFKQSKLEAIRAKYTENQ  IN 
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRN-KSFLVNYTVNLRPKMNPPLPHDAFGNYYFN 300
           NRPSRIESLTAFVYSRFLAAFKHDSAIRN  SFLVNYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVINGE 360
           VM+FPSPETLNDDENCY LVKQLREE NKIDGEMAKKFLNEDKDLMETVKEVASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 LISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQHM 420
           +ISCAFSS+CR PLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEG+DAIVHLEE++M
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLEG 425
           NKLEG
Sbjct: 421 NKLEG 425

BLAST of PI0024010 vs. NCBI nr
Match: XP_038886173.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 801.2 bits (2068), Expect = 4.5e-228
Identity = 389/444 (87.61%), Postives = 412/444 (92.79%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLEVDIISEELIKPSTPTPPHLR Y LS IDQ+TVHIYAP LYFY+STD+G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRRYPLSLIDQITVHIYAPTLYFYRSTDSGSGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           ALAFKNRLRSTLPDVL+HYP LAGRPNYASSFI+CND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPQLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VK VFKPHMEGAKIFP KTMSYD AM + RNRV+RRFVF+QSKLEAIRAKY ENQA IN 
Sbjct: 181 VKDVFKPHMEGAKIFPAKTMSYDTAMTLVRNRVARRFVFEQSKLEAIRAKYAENQATINQ 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNK-SFLVNYTVNLRPKMNPPLPHDAFGNYYFN 300
            RPSRIESL+AFVYSRF+AA KH SA+ N  SFL+NYTVNLRPKMNPPLPHDAFGNYYFN
Sbjct: 241 IRPSRIESLSAFVYSRFIAALKHGSAMSNDVSFLINYTVNLRPKMNPPLPHDAFGNYYFN 300

Query: 301 VMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVINGE 360
           VM+FPSPE LN DENCYGLVKQLREE NKI+GEMAKKFLNE+KDLMETVK+ ASKV+NGE
Sbjct: 301 VMIFPSPEMLNTDENCYGLVKQLREETNKINGEMAKKFLNENKDLMETVKDFASKVVNGE 360

Query: 361 LISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQHM 420
           +ISCAFSS CRLPLYDVDFGWGRPVWVTFP LWFKNLVAFL+SKDG+GIDAIVHLEEQ+M
Sbjct: 361 IISCAFSSTCRLPLYDVDFGWGRPVWVTFPGLWFKNLVAFLESKDGDGIDAIVHLEEQYM 420

Query: 421 NKLEGDEVFMKYATPIPTPSSPLA 444
           NKLE DEVF+KYA  IPTPSSPLA
Sbjct: 421 NKLEADEVFLKYAATIPTPSSPLA 444

BLAST of PI0024010 vs. NCBI nr
Match: KAG7020113.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 665.6 bits (1716), Expect = 2.9e-187
Identity = 326/445 (73.26%), Postives = 375/445 (84.27%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDF 60
           MKLE+DIISE+LIKP+TPTPPHLR Y LSFIDQ+TVHIYAP LYFYQ    G    D DF
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAG----DPDF 60

Query: 61  ALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120
           A+ F+NRLRSTL  VL+HYPPLAGRPNY S+F+DCNDTG  FREARVN++LADV+++A+P
Sbjct: 61  AIDFRNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARP 120

Query: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIAD M LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQAMINL 240
           VKGVFKPHMEGAK+FP KTM+YD A  I RNRV+RRFVF+ SK+EAIR KY EN A    
Sbjct: 181 VKGVFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---A 240

Query: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIR---NKSFLVNYTVNLRPKMNPPLPHDAFGNYY 300
           +RPSR+ESLTAF+Y RFLAA K  S+     +++ LVNYTVNLR KM PPLP DAFGNYY
Sbjct: 241 HRPSRVESLTAFMYGRFLAALKDGSSSEVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYY 300

Query: 301 FNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVIN 360
           FN M+FPSP+ LN D  CYGLV +LREE NK++ +  K  LNED+ LME+V+E+ASK + 
Sbjct: 301 FNAMIFPSPDILN-DPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVK 360

Query: 361 GELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEEQ 420
           GE+ISCAF+S+CR PLYDVDFGWGRPVWVTFPALWFKNLV FLDSK+  GIDAIVHLEE 
Sbjct: 361 GEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEP 420

Query: 421 HMNKLEGDEVFMKYATPIPT-PSSP 442
           HMNKL+ D++F++YATP PT P +P
Sbjct: 421 HMNKLQADQLFLQYATPTPTAPPTP 437

BLAST of PI0024010 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 298.9 bits (764), Expect = 6.7e-81
Identity = 159/441 (36.05%), Postives = 261/441 (59.18%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLR-HYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLD 60
           M LE+ + S+EL+KPS     H   H+ LSF+DQL   I+ P L+FY +  N    E  D
Sbjct: 1   MGLEITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERSD 60

Query: 61  FALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQ 120
                   ++S+L ++L  Y PLAGR   +   + CND GV F EA+ +  ++ +++   
Sbjct: 61  -------HIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPN 120

Query: 121 PDDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMAR 180
           P++LN+L P E    ++  + VQ T F CGG+A+   +SHK+ D ++     N+WAA AR
Sbjct: 121 PNELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFAR 180

Query: 181 G-VKGVFKPHMEGAKIFPPKTMS--YDAAMMIARNRVSRRFVFKQSKLEAIRAKYTENQA 240
           G    +  P  + AK+FPP  +     A  +   N V+RRFVF +S +E++R +++ N+ 
Sbjct: 181 GQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKK 240

Query: 241 MINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPHDAFGNY 300
           +    R +R+E L+ F++SRF+A+  HD     K + + + VNLR + +P +P + FGN 
Sbjct: 241 I----RATRVEVLSVFIWSRFMASTNHDDK-TGKIYTLIHPVNLRRQADPDIPDNMFGNI 300

Query: 301 YFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKEVASKVI 360
               +  P      +DE    LV Q+REE  KID    KK   +++  +E + + AS  +
Sbjct: 301 MRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFV 360

Query: 361 NGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEE 420
           NGE++S +F+S+C+ P+Y+ DFGWG+P+WV    + +KNLVAF+D+K+G+GI+A ++L++
Sbjct: 361 NGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQ 420

Query: 421 QHMNKLEGDEVFMKYATPIPT 438
             M++ E DE  ++Y +  P+
Sbjct: 421 NDMSRFEADEELLRYVSSNPS 429

BLAST of PI0024010 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-67
Identity = 149/452 (32.96%), Postives = 248/452 (54.87%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDFAL 62
           + VD++S ++IKPS+PTP HL+ + LS ++QL   I+ P+++FY + ++    E L    
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQM-- 60

Query: 63  AFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDD 122
                L+ +L + L H+ PLAGR    +  IDCND+G  F EARVNS L++++     D 
Sbjct: 61  -----LKKSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDS 120

Query: 123 LNRLFPVELDRF--NEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAM-AR 182
           L +L P  +D       L+  Q + F CG +++  CISHK+AD  ++     +WAA+ +R
Sbjct: 121 LQQLIPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSR 180

Query: 183 G-VKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRV------SRRFVFKQSKLEAIRAKYT 242
           G +K +  P  +  KIFPP   S  +   +    +      S+RF+F  S ++A++AK  
Sbjct: 181 GSIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAK-- 240

Query: 243 ENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPHDA 302
              +   +N+P+R+E+++A ++   + A +  S     S L N +V+LR +++PP   ++
Sbjct: 241 --ASSFEVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILAN-SVSLRSRVSPPFTKNS 300

Query: 303 FGNYYFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFL---NEDKDLMETVK 362
            GN   +     + E +N  +    LV ++R+ K +       K +   N  + +    K
Sbjct: 301 IGN-LVSYFAAKAEEGINQTK-LQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQK 360

Query: 363 EVASKVINGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGID 422
           E    + +G+     FSS CR  LY+ DFGWG+PVWV FP++  KN+V  LD+K+  GI+
Sbjct: 361 EAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIE 420

Query: 423 AIVHLEEQHMNKLEGDEVFMKYATPIPTPSSP 442
           A V+L EQ MN  E D   +++A+  P+   P
Sbjct: 421 AWVNLNEQEMNLFEQDRELLQFASLNPSVIQP 437

BLAST of PI0024010 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 210.3 bits (534), Expect = 3.2e-54
Identity = 144/448 (32.14%), Postives = 233/448 (52.01%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQS-TDNGPGGEDLD 60
           M+  V+I+S E++KPS+PTP   R  +LS +D L+  +Y   L FY +   N  G    +
Sbjct: 1   MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEE 60

Query: 61  FALAFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQ 120
            +L  K  L  TLP     + PLAGR     SF++CND G  F EARV+  L++ ++   
Sbjct: 61  TSLKLKKSLSKTLP----IFYPLAGR--IIGSFVECNDEGAVFIEARVDHLLSEFLKCPV 120

Query: 121 PDDLNRLFPVEL---DRFNEELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAA 180
           P+ L  L PVE    +     ++ +Q   F+CGG+ +  C+SHKI D  +L      WA 
Sbjct: 121 PESLELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAE 180

Query: 181 MARGVKGVFKPHMEGAKIFPPKTMSYDAAMMIARNR------VSRRFVFKQSKLEAIRAK 240
            +RG+     P    +++FP       +  M  +        V++RFVF  SK++ +RAK
Sbjct: 181 SSRGLGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAK 240

Query: 241 YTENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPH 300
            + N     +  P+R+E++TA ++ R +      S++  ++ ++   VNLR K++  L  
Sbjct: 241 ASRNL----VKNPTRVEAVTA-LFWRCVTKVSRLSSLTPRTSVLQILVNLRGKVD-SLCE 300

Query: 301 DAFGNYYFNVMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETVKE 360
           +  GN   ++M+  + E     E    +V ++R  K +I     K+       + E ++E
Sbjct: 301 NTIGN-MLSLMILKNEEAA--IERIQDVVDEIRRAK-EIFSLNCKEMSKSSSRIFELLEE 360

Query: 361 VASKVING-ELISCAFSSVCRLPLYDVDFGWGRPVWVTFPAL-WFKNLVAFLDSKDGEGI 420
           +      G E+     +S C+L LYD DFGWG+PVWVT      FKNL+  +D+KDGEGI
Sbjct: 361 IGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGI 420

Query: 421 DAIVHLEEQHMNKLEGDEVFMKYATPIP 437
           +A + L E+ M+  E D+  ++ A+  P
Sbjct: 421 EAWITLTEEQMSLFECDQELLESASLNP 432

BLAST of PI0024010 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 206.5 bits (524), Expect = 4.6e-53
Identity = 146/450 (32.44%), Postives = 227/450 (50.44%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDFAL 62
           ++V+ I++E+IKPS+ TP  L+   LS  D +   +Y     FY         +DL    
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTK-------DDLISPE 63

Query: 63  AFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNS-QLADVIQFAQPD 122
              ++L+++L + L  + PLAGR    +  IDCND G  F +ARVN+  L+D ++     
Sbjct: 64  QSSHKLKTSLAETLTKFYPLAGRIKGVT--IDCNDEGAVFVDARVNNYPLSDFLRSPDFK 123

Query: 123 DLNRLFPVEL--DRFNE----ELMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWA 182
            L +L P+++  D +       L+ V+ T F CGG+A+  CI+HKIAD  ++ +   +WA
Sbjct: 124 TLQQLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWA 183

Query: 183 AMARGVKG--VFKPHMEGAKIFPPKTMSYDAAMMIARNRVS---RRFVFKQSKLEAIRAK 242
           AMARG  G  V  P    A  +PP    +   +    N++S   +RFVF  SKLE +R K
Sbjct: 184 AMARGEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTK 243

Query: 243 YTENQAMINLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPPLPH 302
                    + RP R+ES+TA ++  F+AA   ++   +   L+  + NLRPK+   L  
Sbjct: 244 AASEDF---VGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPS-NLRPKIPSLLAE 303

Query: 303 DAFGNYYFN--VMVFPSPETLNDDENCYGLVKQLREEKNKIDGEMAKKFLNEDKDLMETV 362
              GN  F+  V+     E +  ++    L KQ  + +N I  E           L   +
Sbjct: 304 SLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLM 363

Query: 363 KEVASKVINGELISCAFSSVCRLPLYDVDFGWGRPVWVT-FPALWFKNLVAFLDSKDGEG 422
               SK+          SS C+LPLY+  FGWG PVW+    A   +N+   +DSKDG+G
Sbjct: 364 LTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQG 423

Query: 423 IDAIVHLEEQHMNKLEGDEVFMKYATPIPT 438
           I+A V L E++M  LE +   + +A+  P+
Sbjct: 424 IEAFVTLPEENMLSLEQNTELLAFASVNPS 440

BLAST of PI0024010 vs. TAIR 10
Match: AT5G23970.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.3e-52
Identity = 141/444 (31.76%), Postives = 232/444 (52.25%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQLTVHIYAPVLYFYQSTDNGPGGEDLDFAL 62
           ++++++S+E+IKP++P   HL+  SLS  DQ     Y   ++FY   D+    ED+    
Sbjct: 1   MKLELLSKEVIKPASPN--HLQTLSLSLFDQFLPSTYVSAIFFY---DDHSNQEDI---- 60

Query: 63  AFKNRLRSTLPDVLAHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDD 122
               RL+S+L   L+ + PLAG+     + + CND G  F EAR    L+D ++     D
Sbjct: 61  ---QRLKSSLSQTLSLFYPLAGQIKDGVT-VHCNDEGALFTEARAEIFLSDFLRNPSDAD 120

Query: 123 LNRLFPVELDRFNEE---LMAVQFTEFACGGVAVASCISHKIADGMALFSLNNNWAAMAR 182
           L + F V  D  + E   L+ V+   F   G AVA  +SHKI D  +L +   +W   ++
Sbjct: 121 LIQKFIVSPDHADPETWPLLHVKVIFFKDKGFAVAVSVSHKICDAASLSTFVCSWTKASK 180

Query: 183 GVKGVFKPHMEGAKIFPPKTMSYDAAMMIARNRVS--RRFVFKQSKLEAIRAKYTENQAM 242
           G      P   GA  +PP  +S +   ++     S  +RFVF    +E ++ + +  +  
Sbjct: 181 GYADTVDPEFVGADFYPPADISIEFPPLLVHETKSKTKRFVFGSLMIEKLKNRASSGK-- 240

Query: 243 INLNRPSRIESLTAFVYSRFLAAFKHDSAIRNKSFLVNYTVNLRPKMNPP-LPHDAFGNY 302
             + + +RIES+TA +  R +    H  + + K F +  T++LRP+++   LPH A GN+
Sbjct: 241 -RVPQATRIESITALLL-RCMTKAGHSKSGKVKEFAITQTMDLRPRVSSSLLPHKAIGNF 300

Query: 303 YFNVMVFPSPETLNDDENCYGLVKQLREEKNKI---DGEMAKKFLNEDKDLMETVKEVAS 362
           +F  ++  S E+  + E     +++ ++E N++   D E AK  + E K+ + +    + 
Sbjct: 301 FFLPLLKESSESKMEIEETVSKLQKTKQELNELIRNDSEDAKSSV-EAKERIASAMLSSL 360

Query: 363 KVINGELISCAFSSVCRLPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVH 422
             I+ E+ + A SS CR+  Y+ +FGWG+PVWV  P    K  V  +DSKD EG++A V 
Sbjct: 361 CEISPEMETYAVSSWCRMSFYEANFGWGKPVWVA-PDSVDKTQVVLMDSKDSEGVEARVT 420

Query: 423 LEEQHMNKLEGDEVFMKYATPIPT 438
           L E  M K E D   + YATP P+
Sbjct: 421 LPETDMAKYEHDSELLVYATPSPS 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2P1GIW71.5e-6135.57Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR72.4e-5434.41Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
Q9ZTK53.0e-4931.64Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1... [more]
Q8GZU01.3e-4731.15Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=M... [more]
A0A2K8FQU58.1e-4730.43Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
Match NameE-valueIdentityDescription
A0A5A7UYQ78.2e-24493.69Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
A0A0A0LQS83.2e-24092.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1[more]
A0A1S3BD013.8e-23393.88vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1[more]
A0A6J1KPC81.9e-18773.03vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1[more]
A0A6J1GI322.4e-18773.26vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0061032.11.7e-24393.69vinorine synthase-like [Cucumis melo var. makuwa][more]
XP_004143048.16.7e-24092.13stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical prot... [more]
XP_008445555.17.9e-23393.88PREDICTED: vinorine synthase-like [Cucumis melo][more]
XP_038886173.14.5e-22887.61stemmadenine O-acetyltransferase-like [Benincasa hispida][more]
KAG7020113.12.9e-18773.26Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G24430.16.7e-8136.05HXXXD-type acyl-transferase family protein [more]
AT3G26040.11.5e-6732.96HXXXD-type acyl-transferase family protein [more]
AT1G24420.13.2e-5432.14HXXXD-type acyl-transferase family protein [more]
AT3G30280.14.6e-5332.44HXXXD-type acyl-transferase family protein [more]
AT5G23970.11.3e-5231.76HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 4..426
e-value: 1.7E-71
score: 241.1
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..208
e-value: 5.7E-53
score: 181.4
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 210..429
e-value: 4.0E-52
score: 178.4
NoneNo IPR availablePANTHERPTHR31623:SF46ANTHRANILATE N-BENZOYLTRANSFERASEcoord: 2..434
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 2..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0024010.1PI0024010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups