PI0023855 (gene) Melon (PI 482460) v1

Overview
NamePI0023855
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectate lyase
Locationchr05: 17082152 .. 17085335 (-)
RNA-Seq ExpressionPI0023855
SyntenyPI0023855
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCACTTAGTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTCTTCCTAACAATCGCTCTCGTTCGCAAAGGAGAGACCTCCAAAATTAGGTACGTTTTCTTTTTCTCTCTTAGAAATCTCAACTAGATGTGTTTTATTTGGTAATTAATTCTAGCCACACTGTGTGAATTTCTGGTTACGAGTAGTAAATGCAAGCTTCGTATAGTACTGAACTCTTTTTCGATTGGGAGAATCTTGAATGCTCAATGTGGAATTATGAATGTATGATTTAGTGTATTTTTCAGATGATTTTGGATCAATGTAGCAATATAGAAATGTAATAGAGAAGAAGTAAAAACTTCGTTCCAGTGCTTTAATGGCGTCCTTAAGTTCAACATTCTTTTCAGAAAAGAAATGAGGAATAACGCAAGAATCTCTAATTCGAAGCGCGAACTGCGGTGGCTATGTTTTTTATTCCTCTTATTATTATTTTTTTAAAACAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTATAGCAACTCATCAATGGCGACTAGGTCAGTGTTCGTTTCATTAAACTCAAGTAAATAAAAAGAGGTGAAATTCTGTACCGAACGAAACGATCTCGGCCGTGCGTTTTACCCATCTTGCTAATCACAACCGTTGGATTCTCCTCGGATCCGTGTGGTGTGATGATCCAACTGTAGTTAACGATCTCAACCGTTGAGATCCGGATCCTTGATCTGGACCGTTTGTTCCGTTAACAGTTACTCCCTCTGCTGCAGTGAAATAAATACCACATTTTATTTTCGTAATTACCCCAATAGGTGTTCTTAATTTCCCCAGATTGTTCGATAAAATTCCGGGGTATTTCAGTCATCTTACCCCTGAGAATAAAAGGACAATTTTCTTTTTCTTTTTTTCTTTTTAATTCTTTAGACAGTCGTTTCTCATTTTCCCCATCTCGTGCCGACCAATCGTTCAGCCTCTAAATTCTCTCTCTAAACAAAAGCACTTTTTCAAACGGTTTTCTTCTTCCTGACAGAACGACAGTACGGTAAGGCGCGTAAGAGATCTCTTCCGCACCCACCTTGAGCGTCGAGCCATTAACTTTTTCATTTCATTTTCATTCCTCACGCGCCCACTGATATTAACTCGCTTCTTACACTTAGAAATCTGTTCAGTGTGTTCTAACAGTTCTAATATTATTGATTATTAGATGGGTTTGTCACTTAATTGGGCGTTTCTGTTTTCAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCCGATGAGATTGCTGCTTCTGTTGAGATGTGGGTCTTTTTCTTTCATTGCTTCTATACTCGAATTTATGAATTTTTTTTTTAAATCTGGGTTATCATTACATGCTTAAATCTTGTTTAAGGAAGTCATCATTAGACTTTAGAGAAATTACTTTTTTTGAAATTTGGGTCGAATTGGATTTTGTGCTCGTTGTAATGAATAGAACACATTGCGGATGGTGGGCTTTGTTTTTGTCTGTATAGCAAATTCATAGGATGGGTATTTCTAGACGTTATAATGAAAAGAGTTCTCATGATCAAAATGGCAGCATGGTTTTGTCTTGTTCTGTTCGTGTAGTATGGCATTTTCTTATAGCCGTTGGGGATGAAAAATCAGCCCTACCGATTCCCTGTCATTAGGTTCCCGACAAATCCTGTTTTTTCTTTTTATTTTTTCCTCGAGATTTGTACTTGTATCAAGTGTTTTCACTACAATTTCAAGGAGAGAGTTCATATCAGGATCCTTTTTTGATATGGCAGGAGTATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGCGGCATTGGATTTGGTCGGAACGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACCATGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGACATGGTGATTCAATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTTCACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCAGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGATGGACTTGTCGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTACACTTTTTATTGCATTTCTTATATGAAATTAAGTGAAAACACAAATTTATCTGATGTAATTGCATCTTTAACAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTGGAGAAGGACTCATTCAAAGAATGCCAAGGTAAATATAAATAGGTATCAATGGTTTGAATTGTGTTGAGTTGTTTATTACCTGAGATTTCACCTCACATATTTTCAATAATGTATAGATGTAGGCATGGCTATTTCCATGTAGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACAAACCTTTTGCCAAAGAGTACACTCTTCCCGAACTCATCTCAAGCTTAGATTTAGATTCCGATATTGGTTAGCGCAGTTGACAAATCATATCGATCAATACAATACAGGTGACAAAGCGAGTCGAAACACCAGAGAGCAAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGTTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCCAAATCTGCTTCCATGGTTGGATCCATCACTTCTTCTGCAGGTTCACTCCCTTGCCGCCGAGGCCATCCATGCTAG

mRNA sequence

ATGGCGGCACTTAGTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTCTTCCTAACAATCGCTCTCGTTCGCAAAGGAGAGACCTCCAAAATTAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTATAGCAACTCATCAATGGCGACTAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCCGATGAGATTGCTGCTTCTGTTGAGATGAGTATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGCGGCATTGGATTTGGTCGGAACGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACCATGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGACATGGTGATTCAATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTTCACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCAGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGATGGACTTGTCGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTGGAGAAGGACTCATTCAAAGAATGCCAAGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACAAACCTTTTGCCAAAGATCGAAACACCAGAGAGCAAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGTTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCCAAATCTGCTTCCATGGTTGGATCCATCACTTCTTCTGCAGGTTCACTCCCTTGCCGCCGAGGCCATCCATGCTAG

Coding sequence (CDS)

ATGGCGGCACTTAGTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTCTTCCTAACAATCGCTCTCGTTCGCAAAGGAGAGACCTCCAAAATTAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTATAGCAACTCATCAATGGCGACTAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCCGATGAGATTGCTGCTTCTGTTGAGATGAGTATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGCGGCATTGGATTTGGTCGGAACGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACCATGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGACATGGTGATTCAATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTTCACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCAGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGATGGACTTGTCGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTGGAGAAGGACTCATTCAAAGAATGCCAAGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACAAACCTTTTGCCAAAGATCGAAACACCAGAGAGCAAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGTTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCCAAATCTGCTTCCATGGTTGGATCCATCACTTCTTCTGCAGGTTCACTCCCTTGCCGCCGAGGCCATCCATGCTAG

Protein sequence

MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHLEKDSFKECQVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNLLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC
Homology
BLAST of PI0023855 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.2e-182
Identity = 321/422 (76.07%), Postives = 356/422 (84.36%), Query Frame = 0

Query: 34  IRTVSAAEFQSYSNSSM-ATRVENYDVEQELNNEHAV-DNP----DEIAASVEMSIRNST 93
           IR +   EFQS ++S+M ATR++    +Q    +HAV D+P    DE+A  V+MS +N T
Sbjct: 53  IRKLKTEEFQSLNSSTMAATRLDGEPQQQ----QHAVADDPDMVADEVAKLVQMSEQNRT 112

Query: 94  ERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSD 153
            RRKLGFFSCGTGNPIDDCWRCD NWH+NRKRLA+CGIGFGRNAIGGRDGRFY+VTD +D
Sbjct: 113 ARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTD 172

Query: 154 HDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACI 213
            D VNPKPGTLRHAVIQE+PLWIVFKRDMVI+LKQELIMNSFKTIDARG NVHIANGACI
Sbjct: 173 EDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACI 232

Query: 214 TIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLS 273
           TIQF+TN+IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDA+SIFGSSHIWIDHNSLS
Sbjct: 233 TIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLS 292

Query: 274 NCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHLEK---DS 333
           +CADGLVDAVMGSTAIT+SNNHFTHHNEVMLLGHSDSYTKDK MQVTIAYNH  +     
Sbjct: 293 HCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQR 352

Query: 334 FKECQ------VNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNLLPK-----IETPESK 393
              C+      VNNDYTHWEMYAIGGSA PTINSQGNRYAAP +   K     +ET  S+
Sbjct: 353 MPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASE 412

Query: 394 WKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGH 436
           WK WNWRSEGD+LLNGA+F PSGAGASASY RASSL AK +SMV +ITS+AG+L CR+G 
Sbjct: 413 WKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGR 470

BLAST of PI0023855 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 632.1 bits (1629), Expect = 4.6e-180
Identity = 322/453 (71.08%), Postives = 354/453 (78.15%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MA L  W    L  + LLFF                V A E  ++ N S   R +  +  
Sbjct: 1   MAVLPTW---LLAMMCLLFF----------------VGAMENTTHDNISSLPRSDETEW- 60

Query: 61  QELNNEHAVDNPDEIA----ASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
               N+HAV NPDE+A    A  EMS+RN TERRKLG+F+CGTGNPIDDCWRCD NWH+N
Sbjct: 61  ----NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D +PVNP+PGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 180

Query: 181 VIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VIQLKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
            +HFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSAN 360
           MLLGHSDSY +DK MQVTIAYNH           C+      VNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP N     +  +++TP S WKGWNWRSEGD+L NGAYFT SGA AS S
Sbjct: 361 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           YARASSL AKS+S+VG ITS AG+LPCRRG  C
Sbjct: 421 YARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of PI0023855 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 3.2e-173
Identity = 298/394 (75.63%), Postives = 330/394 (83.76%), Query Frame = 0

Query: 56  NYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHR 115
           N     E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  
Sbjct: 23  NASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQL 82

Query: 116 NRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRD 175
            RKRLA+C IGFGRNAIGGRDGRFYVVTD  D DPVNP PGTLRHAVIQ++PLWI+FKRD
Sbjct: 83  RRKRLADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRD 142

Query: 176 MVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRS 235
           MVI LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRS
Sbjct: 143 MVITLKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRS 202

Query: 236 SPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNE 295
           SPSH+G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNE
Sbjct: 203 SPSHYGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE 262

Query: 296 VMLLGHSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSA 355
           VMLLGHSDSYT+DK MQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSA
Sbjct: 263 VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 322

Query: 356 NPTINSQGNRYAAPTNLLPKIETP-----ESKWKGWNWRSEGDMLLNGAYFTPSGAGASA 415
            PTINSQGNR+ AP N   K  T      ESKWK WNWRSEGD+ LNGA+FT SGAGA A
Sbjct: 323 GPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGA 382

Query: 416 SYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           +YARASSL AKS+S+VG++TS +G+L CR G  C
Sbjct: 383 NYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of PI0023855 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 5.2e-171
Identity = 292/385 (75.84%), Postives = 324/385 (84.16%), Query Frame = 0

Query: 65  NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECG 124
           +EHAV+NP+E+AA V+MSIRNST RR+LG+FSC TGNPIDDCWRCD  W   RK LA C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 125 IGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQEL 184
           IGFGRNAIGGRDGR+YVV+D +D +PVNPKPGTLRHAVIQE+PLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 185 IMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRT 244
           IMNSFKTID RGVNVHIANGACITIQFVTNIIIHG+HIHDC+PTGNAMVRSSPSH+GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 245 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDS 304
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITISNN+FTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 305 YTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTINSQGN 364
           YT+DK MQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 365 RYAAPTNLLPKIETPES-----KWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLG 424
           RY AP N   K  T        +W+ WNWRSEGD+ LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 425 AKSASMVGSITSSAGSLPCRRGHPC 436
           AKS+S+VG IT +AG+L CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

BLAST of PI0023855 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 599.4 bits (1544), Expect = 3.3e-170
Identity = 304/435 (69.89%), Postives = 342/435 (78.62%), Query Frame = 0

Query: 15  LALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVEQELNNEHAVDNPDE 74
           LAL   L I  +    ++  +        S SNSS A ++ N D      NEHAV NP+E
Sbjct: 9   LALSTTLIILALCINSSTMAQETEDLNSHSSSNSSTANKLPNDDGAW---NEHAVKNPEE 68

Query: 75  IAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGG 134
           +AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH  RKRLA C IGFGRNAIGG
Sbjct: 69  VAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGG 128

Query: 135 RDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDA 194
           RDGR+YVVTD SDHD VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSFKTID 
Sbjct: 129 RDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDG 188

Query: 195 RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIF 254
           RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSPSH+GWRTMADGDAISIF
Sbjct: 189 RGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIF 248

Query: 255 GSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVT 314
           GSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK MQVT
Sbjct: 249 GSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVT 308

Query: 315 IAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNLLP 374
           IAYNH  +        C+      VNNDYTHW MYAIGGSANPTINSQGNR+ AP N   
Sbjct: 309 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFA 368

Query: 375 KIETP-----ESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSI 434
           K  T      + +WK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS+V  +
Sbjct: 369 KEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSML 428

Query: 435 TSSAGSLPCRRGHPC 436
           T S+G+L CR G  C
Sbjct: 429 TYSSGALKCRIGMRC 440

BLAST of PI0023855 vs. ExPASy TrEMBL
Match: A0A0A0LI24 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G155600 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.4e-235
Identity = 416/449 (92.65%), Postives = 423/449 (94.21%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRK ETSKIRTVSAAEFQS SNSSMATRVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNLLPK-----IETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN   K     +ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of PI0023855 vs. ExPASy TrEMBL
Match: A0A5D3CXY1 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001060 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.1e-235
Identity = 415/449 (92.43%), Postives = 424/449 (94.43%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALSKWSSLSLFALALLFFLTIALVRK ETSKIRTVSAAEFQS S+SSMATRVENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of PI0023855 vs. ExPASy TrEMBL
Match: A0A1S3CDJ9 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103499488 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.1e-235
Identity = 415/449 (92.43%), Postives = 424/449 (94.43%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALSKWSSLSLFALALLFFLTIALVRK ETSKIRTVSAAEFQS S+SSMATRVENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of PI0023855 vs. ExPASy TrEMBL
Match: A0A6J1CB90 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009920 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 2.9e-225
Identity = 399/449 (88.86%), Postives = 416/449 (92.65%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALS+WSSLSL ALA++FF+T+ALVR+ +TS+IRT  AAEFQS SNSSMA RVEN DVE
Sbjct: 1   MAALSRWSSLSLVALAMVFFVTMALVRREQTSEIRTELAAEFQSSSNSSMAARVENDDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
            +L NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  -KLKNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSP+HF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ET ES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 448

BLAST of PI0023855 vs. ExPASy TrEMBL
Match: A0A6J1KD84 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111494562 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.8e-222
Identity = 395/449 (87.97%), Postives = 410/449 (91.31%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALSKWSSLSL ALA++FF+T+ALVRK E S+IRTV A +FQS  NSSMATR  N D+E
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSEIRTVLATKFQSSINSSMATRAVNDDLE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
            ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD NWH  RKRL
Sbjct: 61  -ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSP+HF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ET ES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMV SITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of PI0023855 vs. NCBI nr
Match: XP_004138973.1 (probable pectate lyase 8 isoform X1 [Cucumis sativus] >KGN61525.1 hypothetical protein Csa_005898 [Cucumis sativus])

HSP 1 Score: 825.1 bits (2130), Expect = 2.9e-235
Identity = 416/449 (92.65%), Postives = 423/449 (94.21%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRK ETSKIRTVSAAEFQS SNSSMATRVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNLLPK-----IETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN   K     +ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of PI0023855 vs. NCBI nr
Match: XP_008460726.1 (PREDICTED: probable pectate lyase 8 [Cucumis melo] >KAA0032010.1 putative pectate lyase 8 [Cucumis melo var. makuwa] >TYK16831.1 putative pectate lyase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 823.9 bits (2127), Expect = 6.4e-235
Identity = 415/449 (92.43%), Postives = 424/449 (94.43%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALSKWSSLSLFALALLFFLTIALVRK ETSKIRTVSAAEFQS S+SSMATRVENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of PI0023855 vs. NCBI nr
Match: XP_038906468.1 (probable pectate lyase 8 isoform X1 [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.8e-229
Identity = 405/449 (90.20%), Postives = 418/449 (93.10%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALSKW SLSLFALAL+FF+TIALVRK +TS+IRTV A EFQS SNS MATRV+N DVE
Sbjct: 1   MAALSKWPSLSLFALALVFFVTIALVRKEKTSEIRTVLATEFQSSSNSLMATRVKNDDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLG+FSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGYFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS +HF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSTTHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of PI0023855 vs. NCBI nr
Match: XP_022138849.1 (probable pectate lyase 8 [Momordica charantia])

HSP 1 Score: 790.8 bits (2041), Expect = 6.0e-225
Identity = 399/449 (88.86%), Postives = 416/449 (92.65%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAALS+WSSLSL ALA++FF+T+ALVR+ +TS+IRT  AAEFQS SNSSMA RVEN DVE
Sbjct: 1   MAALSRWSSLSLVALAMVFFVTMALVRREQTSEIRTELAAEFQSSSNSSMAARVENDDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
            +L NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  -KLKNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSP+HF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN     +  ++ET ES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 448

BLAST of PI0023855 vs. NCBI nr
Match: XP_011649115.1 (probable pectate lyase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 786.2 bits (2029), Expect = 1.5e-223
Identity = 399/449 (88.86%), Postives = 406/449 (90.42%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRK ETSKI                  RVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKI------------------RVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSD+DPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNLLPK-----IETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN   K     +ETPES+WKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 431

BLAST of PI0023855 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.1e-184
Identity = 326/442 (73.76%), Postives = 365/442 (82.58%), Query Frame = 0

Query: 14  ALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSM-ATRVENYDVEQELNNEHAV-DN 73
           A+ ++  LT  + R  E S  R +   EFQS ++S+M ATR++    +Q    +HAV D+
Sbjct: 14  AVLVVLALTAMIFRNSEISLSRKLKTEEFQSLNSSTMAATRLDGEPQQQ----QHAVADD 73

Query: 74  P----DEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGF 133
           P    DE+A  V+MS +N T RRKLGFFSCGTGNPIDDCWRCD NWH+NRKRLA+CGIGF
Sbjct: 74  PDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADCGIGF 133

Query: 134 GRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMN 193
           GRNAIGGRDGRFY+VTD +D D VNPKPGTLRHAVIQE+PLWIVFKRDMVI+LKQELIMN
Sbjct: 134 GRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQELIMN 193

Query: 194 SFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMAD 253
           SFKTIDARG NVHIANGACITIQF+TN+IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMAD
Sbjct: 194 SFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMAD 253

Query: 254 GDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTK 313
           GDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+SNNHFTHHNEVMLLGHSDSYTK
Sbjct: 254 GDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTK 313

Query: 314 DKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTINSQGNRYA 373
           DK MQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSA PTINSQGNRYA
Sbjct: 314 DKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYA 373

Query: 374 APTNLLPK-----IETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKS 433
           AP +   K     +ET  S+WK WNWRSEGD+LLNGA+F PSGAGASASY RASSL AK 
Sbjct: 374 APMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKP 433

Query: 434 ASMVGSITSSAGSLPCRRGHPC 436
           +SMV +ITS+AG+L CR+G PC
Sbjct: 434 SSMVDTITSTAGALGCRKGRPC 451

BLAST of PI0023855 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.6e-183
Identity = 321/422 (76.07%), Postives = 356/422 (84.36%), Query Frame = 0

Query: 34  IRTVSAAEFQSYSNSSM-ATRVENYDVEQELNNEHAV-DNP----DEIAASVEMSIRNST 93
           IR +   EFQS ++S+M ATR++    +Q    +HAV D+P    DE+A  V+MS +N T
Sbjct: 53  IRKLKTEEFQSLNSSTMAATRLDGEPQQQ----QHAVADDPDMVADEVAKLVQMSEQNRT 112

Query: 94  ERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSD 153
            RRKLGFFSCGTGNPIDDCWRCD NWH+NRKRLA+CGIGFGRNAIGGRDGRFY+VTD +D
Sbjct: 113 ARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTD 172

Query: 154 HDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACI 213
            D VNPKPGTLRHAVIQE+PLWIVFKRDMVI+LKQELIMNSFKTIDARG NVHIANGACI
Sbjct: 173 EDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACI 232

Query: 214 TIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLS 273
           TIQF+TN+IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDA+SIFGSSHIWIDHNSLS
Sbjct: 233 TIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLS 292

Query: 274 NCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHLEK---DS 333
           +CADGLVDAVMGSTAIT+SNNHFTHHNEVMLLGHSDSYTKDK MQVTIAYNH  +     
Sbjct: 293 HCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQR 352

Query: 334 FKECQ------VNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNLLPK-----IETPESK 393
              C+      VNNDYTHWEMYAIGGSA PTINSQGNRYAAP +   K     +ET  S+
Sbjct: 353 MPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASE 412

Query: 394 WKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGH 436
           WK WNWRSEGD+LLNGA+F PSGAGASASY RASSL AK +SMV +ITS+AG+L CR+G 
Sbjct: 413 WKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGR 470

BLAST of PI0023855 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 632.1 bits (1629), Expect = 3.3e-181
Identity = 322/453 (71.08%), Postives = 354/453 (78.15%), Query Frame = 0

Query: 1   MAALSKWSSLSLFALALLFFLTIALVRKGETSKIRTVSAAEFQSYSNSSMATRVENYDVE 60
           MA L  W    L  + LLFF                V A E  ++ N S   R +  +  
Sbjct: 1   MAVLPTW---LLAMMCLLFF----------------VGAMENTTHDNISSLPRSDETEW- 60

Query: 61  QELNNEHAVDNPDEIA----ASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
               N+HAV NPDE+A    A  EMS+RN TERRKLG+F+CGTGNPIDDCWRCD NWH+N
Sbjct: 61  ----NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D +PVNP+PGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 180

Query: 181 VIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VIQLKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
            +HFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSAN 360
           MLLGHSDSY +DK MQVTIAYNH           C+      VNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTN-----LLPKIETPESKWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP N     +  +++TP S WKGWNWRSEGD+L NGAYFT SGA AS S
Sbjct: 361 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           YARASSL AKS+S+VG ITS AG+LPCRRG  C
Sbjct: 421 YARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of PI0023855 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 609.4 bits (1570), Expect = 2.3e-174
Identity = 298/394 (75.63%), Postives = 330/394 (83.76%), Query Frame = 0

Query: 56  NYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHR 115
           N     E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  
Sbjct: 23  NASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQL 82

Query: 116 NRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRD 175
            RKRLA+C IGFGRNAIGGRDGRFYVVTD  D DPVNP PGTLRHAVIQ++PLWI+FKRD
Sbjct: 83  RRKRLADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRD 142

Query: 176 MVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRS 235
           MVI LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRS
Sbjct: 143 MVITLKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRS 202

Query: 236 SPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNE 295
           SPSH+G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNE
Sbjct: 203 SPSHYGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE 262

Query: 296 VMLLGHSDSYTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSA 355
           VMLLGHSDSYT+DK MQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSA
Sbjct: 263 VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 322

Query: 356 NPTINSQGNRYAAPTNLLPKIETP-----ESKWKGWNWRSEGDMLLNGAYFTPSGAGASA 415
            PTINSQGNR+ AP N   K  T      ESKWK WNWRSEGD+ LNGA+FT SGAGA A
Sbjct: 323 GPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGA 382

Query: 416 SYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 436
           +YARASSL AKS+S+VG++TS +G+L CR G  C
Sbjct: 383 NYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of PI0023855 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 602.1 bits (1551), Expect = 3.7e-172
Identity = 292/385 (75.84%), Postives = 324/385 (84.16%), Query Frame = 0

Query: 65  NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECG 124
           +EHAV+NP+E+AA V+MSIRNST RR+LG+FSC TGNPIDDCWRCD  W   RK LA C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 125 IGFGRNAIGGRDGRFYVVTDSSDHDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQEL 184
           IGFGRNAIGGRDGR+YVV+D +D +PVNPKPGTLRHAVIQE+PLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 185 IMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRT 244
           IMNSFKTID RGVNVHIANGACITIQFVTNIIIHG+HIHDC+PTGNAMVRSSPSH+GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 245 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDS 304
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITISNN+FTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 305 YTKDKQMQVTIAYNHLEK---DSFKECQ------VNNDYTHWEMYAIGGSANPTINSQGN 364
           YT+DK MQVTIAYNH  +        C+      VNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 365 RYAAPTNLLPKIETPES-----KWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLG 424
           RY AP N   K  T        +W+ WNWRSEGD+ LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 425 AKSASMVGSITSSAGSLPCRRGHPC 436
           AKS+S+VG IT +AG+L CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944R12.2e-18276.07Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q940Q14.6e-18071.08Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Q9M8Z83.2e-17375.63Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1[more]
Q93WF15.2e-17175.84Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1[more]
Q9LJ423.3e-17069.89Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LI241.4e-23592.65Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G155600 PE=3 SV=1[more]
A0A5D3CXY13.1e-23592.43Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001060 ... [more]
A0A1S3CDJ93.1e-23592.43Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103499488 PE=3 SV=1[more]
A0A6J1CB902.9e-22588.86Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009920 PE=3 SV=1[more]
A0A6J1KD841.8e-22287.97Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111494562 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004138973.12.9e-23592.65probable pectate lyase 8 isoform X1 [Cucumis sativus] >KGN61525.1 hypothetical p... [more]
XP_008460726.16.4e-23592.43PREDICTED: probable pectate lyase 8 [Cucumis melo] >KAA0032010.1 putative pectat... [more]
XP_038906468.11.8e-22990.20probable pectate lyase 8 isoform X1 [Benincasa hispida][more]
XP_022138849.16.0e-22588.86probable pectate lyase 8 [Momordica charantia][more]
XP_011649115.11.5e-22388.86probable pectate lyase 1 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G13710.21.1e-18473.76Pectin lyase-like superfamily protein [more]
AT4G13710.11.6e-18376.07Pectin lyase-like superfamily protein [more]
AT1G04680.13.3e-18171.08Pectin lyase-like superfamily protein [more]
AT3G07010.12.3e-17475.63Pectin lyase-like superfamily protein [more]
AT5G48900.13.7e-17275.84Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 123..140
score: 54.76
coord: 147..172
score: 65.2
coord: 406..429
score: 36.71
coord: 183..199
score: 64.99
coord: 377..401
score: 53.71
coord: 247..268
score: 69.48
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 175..363
e-value: 1.2E-80
score: 284.0
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 187..321
e-value: 1.7E-10
score: 40.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 100..428
e-value: 1.4E-99
score: 335.4
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 25..432
NoneNo IPR availablePANTHERPTHR31683:SF81PECTATE LYASEcoord: 25..432
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 77..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0023855.1PI0023855.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity