PI0023751 (gene) Melon (PI 482460) v1

Overview
NamePI0023751
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionubiquitin-like-conjugating enzyme ATG10 isoform X1
Locationchr01: 22289626 .. 22292615 (+)
RNA-Seq ExpressionPI0023751
SyntenyPI0023751
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTCAATATCCTTGAAGCTAAAGCATATGATTTCTAGCAACTCAAAAGCAGTTTCCTCAATTATATTGCGTTGTACAATTATCTCCCCAAGGATTGGTAGGTCGGAATCGAGAGCAAGGAGGCATATTGAGGAATGAAGATTAGGAACACATGGAATGGGATGGAGAATTAGGGAATACTTTCTGTTTTCATTCATACAAAATTCTCTCCTATTTTCTCTCAATTTCTCCCCTCTCCCATCATCACCCAGCCGGATCGGTTTCAGTGGTTCCAAACAGGAGTTCAACGCAAGCAATCGTTCGATCACTCAACGATTCGTGTGAATTTCAAGATGGTGAGTATCCCAGATTTTTTTAACTTGTAATGATTTTGATTCTATGTGCCAAGAGCTTGTGATTTTGTTTGTACTTCATATGGGTTTCCAATAATTGCTCAGCCTCCAATTTGTCGTTCCATATTGACGCTTCCCATTTTCGATTTCCCCGTCTCTGTTTCACTGATGGGTTTAGGTTGGTACATGTGAAGCAATAGCTTGGAGTGGAACTATCTCATCAAGTGATTTTTACAAGGCTGCTTCTGATTTTATTCATAGATGGAAACTGATTAACTCTGATTCTCCTTCATGGTCATGGGTTCCATATCAGAAACTACGGTGGATTAGTTCTGATGATAAGGTGAAGTTTGTTCTTCTTTGGTTAAGATATCACTACTTTCACTTTGCTGAGAGTTGACATTTTGCTGTTTCTTAACATATGTATGTAAATGTATATATAAATGCCTTTTTATATGTTAGTAGGTTGAAGGATACTTATCTTTGGAGAAAATATGCCTTCTGAGACCACAAGAGGTCTGCTTCTTCCATTTTTATGGCCCCTTTTTGTTCTTGTTCTGCAATATTATTGTTCTTCATATTCCATTTATTATGTTTTCAACAGAACGAGCAGGAGAAAGGAGGGTGTTTAGAGGAAACAGATATTGATGGGGCTGGCAACAACAATGAGTATCTTGATGAAGCTACGTTTGTATGTGCTTTCTCTAATTGATGTTCTTGTTTGTGGGAACTATACTTCTTTCATTGTGTGATATCTGCTATATATTTGTTAACCAGATTTCTCTTTTTTCTCAAGGTCCCACCCCCAAGTGACCATCAAGAAGTACATTACTATGATTTTCACATTGTGCACTGTGCATCATATAGTGTTCCAGTGCTATACTTTCGAGCTTACTGTTGTGGTATGCAATTTTGAAAGTGGCCTAACAAATGACAAAGTTTTATTGATTCATTATACCTTTAAGAACAGCTTTAGAAAAAAGAATTGATGCAAATTCGAACACTAAGTTTTTAATTACTAGTTATCTTGCAAATAAATAGAGTGACTTGTACTAATGTAATGTTTGGTTGTTGGACCTTGCTAATTCCCTTAGTTATTCTAAAAATGTGATTTAGTGTGACTTGTGTTTCAAATTATGCTATAATATGGTGGTACCAGATATATTATGTGTTAGAATTTTGTTCCTAAGATGAATTATTTAAGCTCATTCAGTATACTAATTTTATAATCTGTACAGATGGACAACCTCTGATGTTGGAAGAAATTGAAAAGGATCTCCCATCACAGTCTGCAGATACATTATTGAATTCAAAATGGACATTTATTACTCAGGAGGTACAACTTTTCTTAGTCAACTTTCTTGGCATTTGATACGAAGTGCACCAAGCTTAAATTGGGTCTCATTGGAAGATAGTCAAATATAGTATGCTTTTTTATTCACATTTTACTATTCAAACAACATAAATATTTGTAAATTATTGTACACATCAGTTTTGGTAAATAGCTAGGTTACATGATATGATACAGCCACAAAAAATGATAGTATCAATGCTTCGAAGGGACTATATGTTTATTCTGAGTGTCTTTTCAGTAATCTTCTGATTGAAAATAAGAATGCAGGATGCTGTTCTTTTCCTTTGGCTTTTTCATTGTGCCGAGTTTGAAAAATTGAATTTCATAGAATTTGTCATATAACTAATTGTGGGTATGGATTAATCTCAGGAGCATCCATATTTAAACAGACCATGGTTCAAATTACATCCTTGTGGAACCAGTGAATGGATGAAGCTACTCTTCCTTAGTGATGCTTCTTGGTTCAATAATGAAATAGCTATTGAAAGATATGTTGCCTCATGGCTCTCAGTTGTGGGGCAAGTAGTTGGCTTGAGGATTCCTATGGAAATGTTGAAAGAAGGCAGTGGCAATCAGCTTTGATTCTGGAGTTCATCAGCATTTCAGCAGCATTTCAGCAGCCTTGGGTAAGTTTGGAGAGTGCTTTTAGCCCATAAAGGCCACATCCCAAAATTTGTCTAGTGTTTCATTATATTAGAAGATTGTTTTTAGAAGAAACTTTAAAATATCAGAAGATTGTTTTTAGAAGAAACTTGCAATTGTAAGAGGTGGGAAGATGTTTTATTCTGTTATTTTTAGCGAGGATGACTCATTTTAAGGGATTTGCTCATGATCTAATTACTGATGGTGGTAATTGGTTTTGGTTCCTAGAAGCAGAACTCCATGCTCTAGTAGAGAAGTCAAAACCTGCCTGAGATGAGAAATAGGCTTTAGCAGGGGGAGGATATCGCACGGAAAGCCGGGCTGCCAAGTTTCGTTTAACGATTGATGTGCACTCCTTTTGAGGTCGGAGGCTTTATCCTTCATTTTTTACAATTGAACTAAAAAAAATTGTCCATTATCATATATATTATCATTCTTTCAAGTTTCACTGTATATCTTGGGAAGTATTTGTGTACATATATGCAAATTTAGGTGATAAAAGTGAATGTGTAAAGGTGAACAATGTTCATTATGGGAGCTTGTAAATATCTCCCTAATTTCACGTTAAAGGATTTATGGACAAATTATGAGTTTGTTATAGAATTTGTTCCTTGTTGGTTTATATTATTCCCTCACAACTTAAACAATGTATAATATGCCGG

mRNA sequence

GTTTCAATATCCTTGAAGCTAAAGCATATGATTTCTAGCAACTCAAAAGCAGTTTCCTCAATTATATTGCGTTGTACAATTATCTCCCCAAGGATTGGTAGGTCGGAATCGAGAGCAAGGAGGCATATTGAGGAATGAAGATTAGGAACACATGGAATGGGATGGAGAATTAGGGAATACTTTCTGTTTTCATTCATACAAAATTCTCTCCTATTTTCTCTCAATTTCTCCCCTCTCCCATCATCACCCAGCCGGATCGGTTTCAGTGGTTCCAAACAGGAGTTCAACGCAAGCAATCGTTCGATCACTCAACGATTCGTGTGAATTTCAAGATGGTTGGTACATGTGAAGCAATAGCTTGGAGTGGAACTATCTCATCAAGTGATTTTTACAAGGCTGCTTCTGATTTTATTCATAGATGGAAACTGATTAACTCTGATTCTCCTTCATGGTCATGGGTTCCATATCAGAAACTACGGTGGATTAGTTCTGATGATAAGGTTGAAGGATACTTATCTTTGGAGAAAATATGCCTTCTGAGACCACAAGAGGTCTGCTTCTTCCATTTTTATGGCCCCTTTTTGTTCTTGTTCTGCAATATTATTGTTCTTCATATTCCATTTATTATGTTTTCAACAGAACGAGCAGGAGAAAGGAGGGTGTTTAGAGGAAACAGATATTGATGGGGCTGGCAACAACAATGAGTATCTTGATGAAGCTACGTTTGTCCCACCCCCAAGTGACCATCAAGAAGTACATTACTATGATTTTCACATTGTGCACTGTGCATCATATAGTGTTCCAGTGCTATACTTTCGAGCTTACTGTTGTGATGGACAACCTCTGATGTTGGAAGAAATTGAAAAGGATCTCCCATCACAGTCTGCAGATACATTATTGAATTCAAAATGGACATTTATTACTCAGGAGGAGCATCCATATTTAAACAGACCATGGTTCAAATTACATCCTTGTGGAACCAGTGAATGGATGAAGCTACTCTTCCTTAGTGATGCTTCTTGGTTCAATAATGAAATAGCTATTGAAAGATATGTTGCCTCATGGCTCTCAGTTGTGGGGCAAGTAGTTGGCTTGAGGATTCCTATGGAAATGTTGAAAGAAGGCAGTGGCAATCAGCTTTGATTCTGGAGTTCATCAGCATTTCAGCAGCATTTCAGCAGCCTTGGCAGAACTCCATGCTCTAGTAGAGAAGTCAAAACCTGCCTGAGATGAGAAATAGGCTTTAGCAGGGGGAGGATATCGCACGGAAAGCCGGGCTGCCAAGTTTCGTTTAACGATTGATGTGCACTCCTTTTGAGGTCGGAGGCTTTATCCTTCATTTTTTACAATTGAACTAAAAAAAATTGTCCATTATCATATATATTATCATTCTTTCAAGTTTCACTGTATATCTTGGGAAGTATTTGTGTACATATATGCAAATTTAGGTGATAAAAGTGAATGTGTAAAGGTGAACAATGTTCATTATGGGAGCTTGTAAATATCTCCCTAATTTCACGTTAAAGGATTTATGGACAAATTATGAGTTTGTTATAGAATTTGTTCCTTGTTGGTTTATATTATTCCCTCACAACTTAAACAATGTATAATATGCCGG

Coding sequence (CDS)

ATGGCCCCTTTTTGTTCTTGTTCTGCAATATTATTGTTCTTCATATTCCATTTATTATGTTTTCAACAGAACGAGCAGGAGAAAGGAGGGTGTTTAGAGGAAACAGATATTGATGGGGCTGGCAACAACAATGAGTATCTTGATGAAGCTACGTTTGTCCCACCCCCAAGTGACCATCAAGAAGTACATTACTATGATTTTCACATTGTGCACTGTGCATCATATAGTGTTCCAGTGCTATACTTTCGAGCTTACTGTTGTGATGGACAACCTCTGATGTTGGAAGAAATTGAAAAGGATCTCCCATCACAGTCTGCAGATACATTATTGAATTCAAAATGGACATTTATTACTCAGGAGGAGCATCCATATTTAAACAGACCATGGTTCAAATTACATCCTTGTGGAACCAGTGAATGGATGAAGCTACTCTTCCTTAGTGATGCTTCTTGGTTCAATAATGAAATAGCTATTGAAAGATATGTTGCCTCATGGCTCTCAGTTGTGGGGCAAGTAGTTGGCTTGAGGATTCCTATGGAAATGTTGAAAGAAGGCAGTGGCAATCAGCTTTGA

Protein sequence

MAPFCSCSAILLFFIFHLLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL
Homology
BLAST of PI0023751 vs. ExPASy Swiss-Prot
Match: Q8VZ52 (Ubiquitin-like-conjugating enzyme ATG10 OS=Arabidopsis thaliana OX=3702 GN=ATG10 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 7.3e-45
Identity = 85/127 (66.93%), Postives = 98/127 (77.17%), Query Frame = 0

Query: 56  PSDHQEVHYYDFHIVHCASYSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWT 115
           P+   E HYYDFHIV+ ASY VPVLYFR YC  G+PL L+ I+KD+PS S   LL SKWT
Sbjct: 99  PTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWT 158

Query: 116 FITQEEHPYLNRPWFKLHPCGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGL 175
           FITQEEHPYLNRPWFKLHPCGT +W+KLL  S +S    ++ I  Y+ SW SVVGQVVGL
Sbjct: 159 FITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSS-SGCQMPIVLYLVSWFSVVGQVVGL 218

Query: 176 RIPMEML 183
           RIP+EML
Sbjct: 219 RIPLEML 224

BLAST of PI0023751 vs. ExPASy Swiss-Prot
Match: Q8R1P4 (Ubiquitin-like-conjugating enzyme ATG10 OS=Mus musculus OX=10090 GN=Atg10 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-23
Identity = 57/137 (41.61%), Postives = 83/137 (60.58%), Query Frame = 0

Query: 53  VPPPSDHQEVHYYDFHIVHCASYSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNS 112
           V  P+   EV  +++H+++  SY VPVLYFRA   DG+PL LE+I + +       LL  
Sbjct: 83  VTRPAAVAEVIKHEYHVLYSCSYQVPVLYFRASFLDGRPLALEDIWEGVHECYKPRLLQG 142

Query: 113 KWTFITQEEHPYLNRPWFKLHPCGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQV 172
            W  ITQ+EHP L +P+F LHPC T+E+M  + L ++   N  +    Y+ SWLS+VG V
Sbjct: 143 PWDTITQQEHPILGQPFFVLHPCKTNEFMTAV-LKNSQKINRNV---NYITSWLSLVGPV 202

Query: 173 VGLRIPMEMLKEGSGNQ 190
           VGL +P+   K  S ++
Sbjct: 203 VGLNLPLSYAKATSQSE 215

BLAST of PI0023751 vs. ExPASy Swiss-Prot
Match: Q9H0Y0 (Ubiquitin-like-conjugating enzyme ATG10 OS=Homo sapiens OX=9606 GN=ATG10 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.6e-23
Identity = 55/129 (42.64%), Postives = 80/129 (62.02%), Query Frame = 0

Query: 61  EVHYYDFHIVHCASYSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQE 120
           EV  Y++H+++  SY VPVLYFRA   DG+PL L++I + +       LL   W  ITQ+
Sbjct: 92  EVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLTLKDIWEGVHECYKMRLLQGPWDTITQQ 151

Query: 121 EHPYLNRPWFKLHPCGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME 180
           EHP L +P+F LHPC T+E+M  + L ++   N  +    Y+ SWLS+VG VVGL +P+ 
Sbjct: 152 EHPILGQPFFVLHPCKTNEFMTPV-LKNSQKINKNV---NYITSWLSIVGPVVGLNLPLS 211

Query: 181 MLKEGSGNQ 190
             K  S ++
Sbjct: 212 YAKATSQDE 216

BLAST of PI0023751 vs. ExPASy Swiss-Prot
Match: Q55EL2 (Ubiquitin-like-conjugating enzyme ATG10 OS=Dictyostelium discoideum OX=44689 GN=atg10 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.2e-19
Identity = 56/168 (33.33%), Postives = 89/168 (52.98%), Query Frame = 0

Query: 36  DIDGAGNNN--------EYLDEA--TFVPPPSDHQE--VHYYDFHIVHCASYSVPVLYFR 95
           +I+   NNN        E +D++  T +   +++ E  +  + F I++  SY VPVLY  
Sbjct: 62  NIENKNNNNIENFEEIKETIDDSSTTIIKSNNNNNENNIIIFQFDIIYSKSYQVPVLYLN 121

Query: 96  AYCC-DGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTSEWMK 155
            +   D  PL   EI  +LP  + D    S   +ITQ EHP L  P ++LHPC T   MK
Sbjct: 122 GFSSFDSSPLSWNEIWNNLPLSNLDKNQQSTIPYITQVEHPILGNPCYQLHPCETDNLMK 181

Query: 156 LLFLSDASWFNN----EIAIERYVASWLSVVGQVVGLRIPMEMLKEGS 187
           L+ L +  + +N    E   + Y+ SWLS++G +V ++IP ++LK  +
Sbjct: 182 LILLKEKDYNDNNDKKEYFKDYYLLSWLSIIGPMVNIKIPFDLLKNNN 229

BLAST of PI0023751 vs. ExPASy Swiss-Prot
Match: A5DVH6 (Autophagy-related protein 3 OS=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) OX=379508 GN=ATG3 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.6e-04
Identity = 36/128 (28.12%), Postives = 61/128 (47.66%), Query Frame = 0

Query: 23  QNEQEKGGCLEETDIDGAGNNNEYL--DEATFVP---PPSDHQEVHYYDFHIVHCASYSV 82
           + E+E+G   EE + +G GN  ++   D+A +         H ++  YD +I +  SY V
Sbjct: 160 EEEEEEG---EEGEGEGNGNGADFHADDDADYDDLDIVQGSHSKLRRYDLYITYSTSYRV 219

Query: 83  PVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYL-NRPWFKLHPCG 142
           P LY   +  +G PL+ +++ +D+ S   D          T E+ P   N     +HPC 
Sbjct: 220 PKLYLVGFDANGIPLLPQQMFEDINSDYKDKT-------ATIEQLPVAHNTTSVSIHPCK 277

Query: 143 TSEWMKLL 145
            S  M++L
Sbjct: 280 HSSVMRVL 277

BLAST of PI0023751 vs. ExPASy TrEMBL
Match: A0A1S3C710 (ubiquitin-like-conjugating enzyme ATG10 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103497629 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.5e-93
Identity = 165/176 (93.75%), Postives = 169/176 (96.02%), Query Frame = 0

Query: 15  IFHLLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCAS 74
           +F LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEAT VPPPSD QEVHYYDFHI+HCAS
Sbjct: 29  LFRLLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATLVPPPSD-QEVHYYDFHILHCAS 88

Query: 75  YSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHP 134
           YSVPVLYFRAYCCDGQ LM EEIEKDLPSQS DTLLNSKWTFITQEEHPYLNRPWFKLHP
Sbjct: 89  YSVPVLYFRAYCCDGQQLMFEEIEKDLPSQSVDTLLNSKWTFITQEEHPYLNRPWFKLHP 148

Query: 135 CGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           CGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME+LKE SG+QL
Sbjct: 149 CGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMELLKEVSGSQL 203

BLAST of PI0023751 vs. ExPASy TrEMBL
Match: A0A1S3C6K7 (ubiquitin-like-conjugating enzyme ATG10 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497629 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 7.7e-90
Identity = 161/173 (93.06%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 18  LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSV 77
           LL  Q+NEQEKGGCLEETDIDGAGNNNEYLDEAT VPPPSD QEVHYYDFHI+HCASYSV
Sbjct: 68  LLRPQENEQEKGGCLEETDIDGAGNNNEYLDEATLVPPPSD-QEVHYYDFHILHCASYSV 127

Query: 78  PVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 137
           PVLYFRAYCCDGQ LM EEIEKDLPSQS DTLLNSKWTFITQEEHPYLNRPWFKLHPCGT
Sbjct: 128 PVLYFRAYCCDGQQLMFEEIEKDLPSQSVDTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 187

Query: 138 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME+LKE SG+QL
Sbjct: 188 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMELLKEVSGSQL 239

BLAST of PI0023751 vs. ExPASy TrEMBL
Match: A0A1S4E1X7 (ubiquitin-like-conjugating enzyme ATG10 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497629 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.9e-88
Identity = 156/172 (90.70%), Postives = 163/172 (94.77%), Query Frame = 0

Query: 19  LCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSVP 78
           +C  + ++EKGGCLEETDIDGAGNNNEYLDEAT VPPPSD QEVHYYDFHI+HCASYSVP
Sbjct: 66  ICLLRPQEEKGGCLEETDIDGAGNNNEYLDEATLVPPPSD-QEVHYYDFHILHCASYSVP 125

Query: 79  VLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTS 138
           VLYFRAYCCDGQ LM EEIEKDLPSQS DTLLNSKWTFITQEEHPYLNRPWFKLHPCGTS
Sbjct: 126 VLYFRAYCCDGQQLMFEEIEKDLPSQSVDTLLNSKWTFITQEEHPYLNRPWFKLHPCGTS 185

Query: 139 EWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           EWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME+LKE SG+QL
Sbjct: 186 EWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMELLKEVSGSQL 236

BLAST of PI0023751 vs. ExPASy TrEMBL
Match: A0A0A0K4P3 (Autophagy_act_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G368220 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.2e-88
Identity = 159/173 (91.91%), Postives = 164/173 (94.80%), Query Frame = 0

Query: 18  LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSV 77
           LL  Q+NE+EKGGCLEE DIDGAG NNEYLDEAT VPPPSDHQEVHYYDFHI+HCASYSV
Sbjct: 68  LLRPQENEEEKGGCLEEADIDGAG-NNEYLDEATLVPPPSDHQEVHYYDFHILHCASYSV 127

Query: 78  PVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 137
           PVLYFRAYCCDGQPLM EEIEK+LPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT
Sbjct: 128 PVLYFRAYCCDGQPLMFEEIEKNLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 187

Query: 138 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           SEWMKLLFLSDAS FNNEIAIERYVASWLSVVGQVVGLRIPMEMLKE  G+QL
Sbjct: 188 SEWMKLLFLSDASLFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEVGGSQL 239

BLAST of PI0023751 vs. ExPASy TrEMBL
Match: A0A6J1GNY4 (ubiquitin-like-conjugating enzyme ATG10 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455801 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.2e-78
Identity = 143/172 (83.14%), Postives = 151/172 (87.79%), Query Frame = 0

Query: 18  LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSV 77
           LL  Q+NEQEKG C EE  ID A NNNE LDEAT V  PSDH+EVHYYDFH++HC+SYSV
Sbjct: 89  LLRPQENEQEKGECFEE--IDTADNNNESLDEATLVSSPSDHEEVHYYDFHVLHCSSYSV 148

Query: 78  PVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 137
           PVLYFRAY CDGQPL  EE++KDLPSQSADTLLNSKWTFITQEEHPYLNR WFKLHPCGT
Sbjct: 149 PVLYFRAYSCDGQPLTFEEMKKDLPSQSADTLLNSKWTFITQEEHPYLNRIWFKLHPCGT 208

Query: 138 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQ 190
            EWMKLL LSDASWF NEIAIERYVASWLSVVGQVVGLRIPMEMLK+G G Q
Sbjct: 209 REWMKLLLLSDASWFKNEIAIERYVASWLSVVGQVVGLRIPMEMLKDGGGTQ 258

BLAST of PI0023751 vs. NCBI nr
Match: XP_008458096.1 (PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X3 [Cucumis melo])

HSP 1 Score: 352.1 bits (902), Expect = 3.1e-93
Identity = 165/176 (93.75%), Postives = 169/176 (96.02%), Query Frame = 0

Query: 15  IFHLLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCAS 74
           +F LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEAT VPPPSD QEVHYYDFHI+HCAS
Sbjct: 29  LFRLLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATLVPPPSD-QEVHYYDFHILHCAS 88

Query: 75  YSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHP 134
           YSVPVLYFRAYCCDGQ LM EEIEKDLPSQS DTLLNSKWTFITQEEHPYLNRPWFKLHP
Sbjct: 89  YSVPVLYFRAYCCDGQQLMFEEIEKDLPSQSVDTLLNSKWTFITQEEHPYLNRPWFKLHP 148

Query: 135 CGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           CGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME+LKE SG+QL
Sbjct: 149 CGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMELLKEVSGSQL 203

BLAST of PI0023751 vs. NCBI nr
Match: XP_008458094.1 (PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X1 [Cucumis melo] >XP_008458095.1 PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X1 [Cucumis melo] >XP_016902227.1 PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X1 [Cucumis melo])

HSP 1 Score: 339.7 bits (870), Expect = 1.6e-89
Identity = 161/173 (93.06%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 18  LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSV 77
           LL  Q+NEQEKGGCLEETDIDGAGNNNEYLDEAT VPPPSD QEVHYYDFHI+HCASYSV
Sbjct: 68  LLRPQENEQEKGGCLEETDIDGAGNNNEYLDEATLVPPPSD-QEVHYYDFHILHCASYSV 127

Query: 78  PVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 137
           PVLYFRAYCCDGQ LM EEIEKDLPSQS DTLLNSKWTFITQEEHPYLNRPWFKLHPCGT
Sbjct: 128 PVLYFRAYCCDGQQLMFEEIEKDLPSQSVDTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 187

Query: 138 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME+LKE SG+QL
Sbjct: 188 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMELLKEVSGSQL 239

BLAST of PI0023751 vs. NCBI nr
Match: XP_016902228.1 (PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X2 [Cucumis melo])

HSP 1 Score: 335.1 bits (858), Expect = 3.9e-88
Identity = 156/172 (90.70%), Postives = 163/172 (94.77%), Query Frame = 0

Query: 19  LCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSVP 78
           +C  + ++EKGGCLEETDIDGAGNNNEYLDEAT VPPPSD QEVHYYDFHI+HCASYSVP
Sbjct: 66  ICLLRPQEEKGGCLEETDIDGAGNNNEYLDEATLVPPPSD-QEVHYYDFHILHCASYSVP 125

Query: 79  VLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTS 138
           VLYFRAYCCDGQ LM EEIEKDLPSQS DTLLNSKWTFITQEEHPYLNRPWFKLHPCGTS
Sbjct: 126 VLYFRAYCCDGQQLMFEEIEKDLPSQSVDTLLNSKWTFITQEEHPYLNRPWFKLHPCGTS 185

Query: 139 EWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           EWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPME+LKE SG+QL
Sbjct: 186 EWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMELLKEVSGSQL 236

BLAST of PI0023751 vs. NCBI nr
Match: XP_004149318.1 (ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >XP_011659214.1 ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >XP_011659215.1 ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >XP_011659216.1 ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >XP_011659217.1 ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >XP_031745024.1 ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >KGN44670.1 hypothetical protein Csa_016275 [Cucumis sativus])

HSP 1 Score: 334.3 bits (856), Expect = 6.7e-88
Identity = 159/173 (91.91%), Postives = 164/173 (94.80%), Query Frame = 0

Query: 18  LLCFQQNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSV 77
           LL  Q+NE+EKGGCLEE DIDGAG NNEYLDEAT VPPPSDHQEVHYYDFHI+HCASYSV
Sbjct: 68  LLRPQENEEEKGGCLEEADIDGAG-NNEYLDEATLVPPPSDHQEVHYYDFHILHCASYSV 127

Query: 78  PVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 137
           PVLYFRAYCCDGQPLM EEIEK+LPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT
Sbjct: 128 PVLYFRAYCCDGQPLMFEEIEKNLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGT 187

Query: 138 SEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           SEWMKLLFLSDAS FNNEIAIERYVASWLSVVGQVVGLRIPMEMLKE  G+QL
Sbjct: 188 SEWMKLLFLSDASLFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEVGGSQL 239

BLAST of PI0023751 vs. NCBI nr
Match: XP_038896256.1 (ubiquitin-like-conjugating enzyme ATG10 isoform X1 [Benincasa hispida])

HSP 1 Score: 313.9 bits (803), Expect = 9.3e-82
Identity = 150/169 (88.76%), Postives = 155/169 (91.72%), Query Frame = 0

Query: 23  QNEQEKGGCLEETDIDGAGNNNEYLDEATFVPPPSDHQEVHYYDFHIVHCASYSVPVLYF 82
           +NEQEKG CLEE D  GAGN NE LDEAT VP PSDHQEVHYYDFHI+H ASYSVPVLYF
Sbjct: 73  ENEQEKGECLEEIDTVGAGNCNESLDEATLVPSPSDHQEVHYYDFHIIHSASYSVPVLYF 132

Query: 83  RAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTSEWMK 142
           RAYCCDGQPLM EEI+KDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTSEWMK
Sbjct: 133 RAYCCDGQPLMFEEIKKDLPSQSADTLLNSKWTFITQEEHPYLNRPWFKLHPCGTSEWMK 192

Query: 143 LLFL-SDASWFNNEIAIERYVASWLSVVGQVVGLRIPMEMLKEGSGNQL 191
           LLFL SDAS F NEIAIERYVASWLSVVGQVVGLRIPMEML +GSG+QL
Sbjct: 193 LLFLSSDASLFKNEIAIERYVASWLSVVGQVVGLRIPMEMLNDGSGSQL 241

BLAST of PI0023751 vs. TAIR 10
Match: AT3G07525.2 (autophagocytosis-associated family protein )

HSP 1 Score: 181.8 bits (460), Expect = 5.2e-46
Identity = 85/127 (66.93%), Postives = 98/127 (77.17%), Query Frame = 0

Query: 56  PSDHQEVHYYDFHIVHCASYSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWT 115
           P+   E HYYDFHIV+ ASY VPVLYFR YC  G+PL L+ I+KD+PS S   LL SKWT
Sbjct: 100 PTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWT 159

Query: 116 FITQEEHPYLNRPWFKLHPCGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGL 175
           FITQEEHPYLNRPWFKLHPCGT +W+KLL  S +S    ++ I  Y+ SW SVVGQVVGL
Sbjct: 160 FITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSS-SGCQMPIVLYLVSWFSVVGQVVGL 219

Query: 176 RIPMEML 183
           RIP+EML
Sbjct: 220 RIPLEML 225

BLAST of PI0023751 vs. TAIR 10
Match: AT3G07525.1 (autophagocytosis-associated family protein )

HSP 1 Score: 181.8 bits (460), Expect = 5.2e-46
Identity = 85/127 (66.93%), Postives = 98/127 (77.17%), Query Frame = 0

Query: 56  PSDHQEVHYYDFHIVHCASYSVPVLYFRAYCCDGQPLMLEEIEKDLPSQSADTLLNSKWT 115
           P+   E HYYDFHIV+ ASY VPVLYFR YC  G+PL L+ I+KD+PS S   LL SKWT
Sbjct: 99  PTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWT 158

Query: 116 FITQEEHPYLNRPWFKLHPCGTSEWMKLLFLSDASWFNNEIAIERYVASWLSVVGQVVGL 175
           FITQEEHPYLNRPWFKLHPCGT +W+KLL  S +S    ++ I  Y+ SW SVVGQVVGL
Sbjct: 159 FITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSS-SGCQMPIVLYLVSWFSVVGQVVGL 218

Query: 176 RIPMEML 183
           RIP+EML
Sbjct: 219 RIPLEML 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZ527.3e-4566.93Ubiquitin-like-conjugating enzyme ATG10 OS=Arabidopsis thaliana OX=3702 GN=ATG10... [more]
Q8R1P41.6e-2341.61Ubiquitin-like-conjugating enzyme ATG10 OS=Mus musculus OX=10090 GN=Atg10 PE=1 S... [more]
Q9H0Y04.6e-2342.64Ubiquitin-like-conjugating enzyme ATG10 OS=Homo sapiens OX=9606 GN=ATG10 PE=1 SV... [more]
Q55EL25.2e-1933.33Ubiquitin-like-conjugating enzyme ATG10 OS=Dictyostelium discoideum OX=44689 GN=... [more]
A5DVH63.6e-0428.13Autophagy-related protein 3 OS=Lodderomyces elongisporus (strain ATCC 11503 / CB... [more]
Match NameE-valueIdentityDescription
A0A1S3C7101.5e-9393.75ubiquitin-like-conjugating enzyme ATG10 isoform X3 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A1S3C6K77.7e-9093.06ubiquitin-like-conjugating enzyme ATG10 isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A1S4E1X71.9e-8890.70ubiquitin-like-conjugating enzyme ATG10 isoform X2 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A0A0K4P33.2e-8891.91Autophagy_act_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G36... [more]
A0A6J1GNY45.2e-7883.14ubiquitin-like-conjugating enzyme ATG10 isoform X1 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
XP_008458096.13.1e-9393.75PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X3 [Cucumis melo][more]
XP_008458094.11.6e-8993.06PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X1 [Cucumis melo] >XP... [more]
XP_016902228.13.9e-8890.70PREDICTED: ubiquitin-like-conjugating enzyme ATG10 isoform X2 [Cucumis melo][more]
XP_004149318.16.7e-8891.91ubiquitin-like-conjugating enzyme ATG10 [Cucumis sativus] >XP_011659214.1 ubiqui... [more]
XP_038896256.19.3e-8288.76ubiquitin-like-conjugating enzyme ATG10 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G07525.25.2e-4666.93autophagocytosis-associated family protein [more]
AT3G07525.15.2e-4666.93autophagocytosis-associated family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PFAMPF03987Autophagy_act_Ccoord: 25..178
e-value: 1.3E-32
score: 113.5
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PANTHERPTHR12866UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 22..181
NoneNo IPR availableGENE3D3.30.1460.50coord: 26..175
e-value: 4.5E-28
score: 100.2
NoneNo IPR availablePANTHERPTHR12866:SF5AUTOPHAGY-RELATED 10, ISOFORM Bcoord: 22..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0023751.1PI0023751.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019787 ubiquitin-like protein transferase activity