PI0023565 (gene) Melon (PI 482460) v1

Overview
NamePI0023565
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionBeta-carotene hydroxylase
Locationchr09: 1777346 .. 1780715 (+)
RNA-Seq ExpressionPI0023565
SyntenyPI0023565
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACCGTGATTTCGGCCGTTGCAACCGCCGGGCCGTACCGGTACGATTACCGGAGGTTGTTTCAACGACCGGCCGGAGCTCCGAGTCCAGGTTTTCCATGGCGGTGTTTTGCAAAACAGAGGTGGAGAAGATTGATGGTTATGAAGACGAAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAAATGAGTGTGGAGGAGAAAATGGGTAAGAAGAAAGCAGAAAGAGAGGGTTATTTCATAGCTGCAATTGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACCGATTCTCTTCGCAAGTAATGGTGGTACTCTGTTTTCATCTTCTTCTTCTTTTTTTTTTTTTAATTAAATTTCATTGATATCTCGATGCATACTAAAAAAAACTTGTCGATGCTATTAAATTATTTTATATCAAATTATAAATTAATACTCAGTGTCATAAGAGATTCAAGGACATTCGAGTCTCCCTTAATTTTATATTCATTAGTTGTGTGTGTATATATCAATATCATTAGTTGACTCAGTAGTAAATTAGTATTTTAGAATCTCTAAATCCGAATCAAGTTCCATTCTTTACATTTTGTTTCATATTATATTTTTACTGAATTATAAAATCCTATCGATAATCTTTATACATAGATATGTCGTAGATTGGTGCATCTAGGTTATACCCATCATTTTGTAGACAAAGCACTCTTTTTTGCATGACAAACCTTTTCCAAAACTAACATTATTTTGTTTTTTTTAGTGAGTTGCTATTTAGGAACTATTTGGAAAACTAAAATATAATTAAGATTATTGGGATTTAGAGAAGTGTTGAAAAAAAATGAGATCATGAAGCGTGGAATAATGAAAAAGAATGTTGAATTGAAACTCATTGGAACAAAACGTTCAATTCAAACATGGATTTTGGATTACATTACATTACATTACAAACTTATTCCATTACAACCCAGAAAATCTCTAGGATGCACTCACTTACTCGTGCATGTATTATTTGAGCAATTTCACGAGACATTTTTGTTGGAAAATGCTCTTTTATATACAACGTGACATCTTTTCATTAAAAAAAATGGTATGCAATTAATGTGGTAAGAAATTTTTTTTTGCATTATGTTGGTATACCCTACCATTTCTATTTACAATTTACAAGTTGTGTGTTAATGACAAGTTTGAGAATTTTGTATTCTGTTTTGGGAGTTTCTCTTCTTTCCTCTTTCAATAATATGATGGATTATGCTCTTTTCTTCTGCTTCATGTTTTTATTTCTGCTTTAGTTTGATCATATGCTTTATATATATATTGGGATCTAACACTTTTAAACTGTTTTTAGGGTATGAAAATTTTATTATTACTCATGTATTCAAATAGATTAATAAAGTGACATAAAGAAATAGTTTGAGAATTTTAAATTGGAGATAAATCTATTTATTTATGTTTCGGGTAAAAAGGAAAGAGCTGAGTTTTATTATGTATTTTGCAGGGCGCAGAATTTCCAATTCTAGAAATGTTCGGGACTTTTGTGCTATCTATTGGTTCTGCTGTGAGTTACGTTGTTTAAATTTAAAATGAATTAAAAGAGATGGTTTGAAACATTTTAAAAACTCATTCAAACCAATTCCGATCCTAAACCATTTTTCATTGATGGCAGGTGGGAATGGAATTTTGGGCGAGATGGGCTCACAAAGAGCTATGGCATGCTTCCTTATGGGATATGCACGAGGTAATTAATAATATACGGTGTTACAATCAATAATTAAAAGTATAGTAAAAATTATTTATTATTTTTTAGTAGTCTCATCACAAGCCGAGGGTTGGAGCATTTGAAAAGAACGATGTGTTTGCAATTATAAACGCAATTCCAGCCATTGCTCTTCTTTCTTTTGGCCTCTTCAATCAAGGCTTCTTTCCCGGCCTTTGTTTCGGTGCCGTACGTCTCTTTCTCCCCTCTTTATTTACTTATATTTTGCTCCTTCCATTTATTTTATTTTATTTTTTCTTCCTCCCTACATTTTAAAAATGCTTATTCTACTTTAGAACTTTTTAAGAACAATTATTTTGCTTGTTATTTTATTTTTATATTAACAAATTAGTGAGTTAATTCTTGAGATTTAAAAATGAACTAACATTTTACTAAATATTTTTAAATAGTTTTTTTTTATTTACAATAATTTTCTTAAATTTTAGCCTTTAAATACCATTGTTACACATAAATTTATGTATTTTGTTATCCATTTTTTATTAATAATTTTAAAAAACTCAATCCAAAATTTTAAAACAAAAAAAAAATATAGTTTCAAAAACTTGTTTTTGTTTTTTTTTTTCCTAAGAATTGTGAAAATATAATAGAAGGACTAGAAAAAATAAAATAGAAATTTCAAAACCAGAAAGCAACCAACAAAATTACGACTAAATCAAACCTAAATAATGTTTTCATCTAAATCTAGAAATAAATTTGTAATTTATCAAATAGAATATCATATAGATTAAATCTTTTTCTTTTTCTTATAAATATGAAGAACCAATATTTAAAGTATTTCCTCCTAATTTTATGAATATCGATGGATGATTCATACTTTCCAAATTCTTCCAACCTAACTTAGTAAAGTGGTGAAGGAAAATGAAGTCCAAGTCAGCTTTTGTGTGACGTGGCGTTCCATGATTGGCCATTATTACAGGGTTTGGGAATTACGGTGTTTGGGATGGCTTACATGTTCGTCCACGATGGCCTCGTTCACCGTCGTTTCCCAGTGGGTCCCATAGCCGCTGTCCCCTACTTACGACGTGTCGCTGCTGCCCATCATGTACTTTTCGACTCGTCGTTAATTTTACCTAATTTTTTTAAATATTTAATATTGTCTTTTCTTTATTTACTTTATTTTAAAATTAATTTTAATAACATTTTCAAATATGAAGAGTAATATTTATGAAACAAATCAAAATTTTAGTTCTGTCACTTACCATAATTTTTTTTAATTTTATTTTTGTATTTTGGCAAAGATTCATCACACAGACAAATTCGACGGAGTTCCATACGGGTTGTTTTTAGGACCCAAGGTTAGTTTCATCGTTTTACTTCTTATCTATCTTATTTTTCTTATCTATCTTATTTTAGTTCTATAATTTCAAAGTTTCTTTTCTTACTTTCTATCATTCTTTTCAATTTTTATTTGGAAGCATATTCATTATATTGGATAAAGGGATTAAGTTTAAAATTTATTAAAAATATATTAATTACTCTAAAACGGCGTCGTTGGTTTTCAGGAACTGGAAGAAGTGGATGGCGAGGAAGAGCTGCAGAAGGAAATTAAAAGGAGGAGAAAGGTTTACAGAAATTAA

mRNA sequence

ATGGCGACCGTGATTTCGGCCGTTGCAACCGCCGGGCCGTACCGGTACGATTACCGGAGGTTGTTTCAACGACCGGCCGGAGCTCCGAGTCCAGGTTTTCCATGGCGGTGTTTTGCAAAACAGAGGTGGAGAAGATTGATGGTTATGAAGACGAAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAAATGAGTGTGGAGGAGAAAATGGGTAAGAAGAAAGCAGAAAGAGAGGGTTATTTCATAGCTGCAATTGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACCGATTCTCTTCGCAAGTAATGGGCGCAGAATTTCCAATTCTAGAAATGTTCGGGACTTTTGTGCTATCTATTGGTTCTGCTGTGGGAATGGAATTTTGGGCGAGATGGGCTCACAAAGAGCTATGGCATGCTTCCTTATGGGATATGCACGAGTCTCATCACAAGCCGAGGGTTGGAGCATTTGAAAAGAACGATGTGTTTGCAATTATAAACGCAATTCCAGCCATTGCTCTTCTTTCTTTTGGCCTCTTCAATCAAGGCTTCTTTCCCGGCCTTTGTTTCGGTGCCGGTTTGGGAATTACGGTGTTTGGGATGGCTTACATGTTCGTCCACGATGGCCTCGTTCACCGTCGTTTCCCAGTGGGTCCCATAGCCGCTGTCCCCTACTTACGACGTGTCGCTGCTGCCCATCATATTCATCACACAGACAAATTCGACGGAGTTCCATACGGGTTGTTTTTAGGACCCAAGGAACTGGAAGAAGTGGATGGCGAGGAAGAGCTGCAGAAGGAAATTAAAAGGAGGAGAAAGGTTTACAGAAATTAA

Coding sequence (CDS)

ATGGCGACCGTGATTTCGGCCGTTGCAACCGCCGGGCCGTACCGGTACGATTACCGGAGGTTGTTTCAACGACCGGCCGGAGCTCCGAGTCCAGGTTTTCCATGGCGGTGTTTTGCAAAACAGAGGTGGAGAAGATTGATGGTTATGAAGACGAAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAAATGAGTGTGGAGGAGAAAATGGGTAAGAAGAAAGCAGAAAGAGAGGGTTATTTCATAGCTGCAATTGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACCGATTCTCTTCGCAAGTAATGGGCGCAGAATTTCCAATTCTAGAAATGTTCGGGACTTTTGTGCTATCTATTGGTTCTGCTGTGGGAATGGAATTTTGGGCGAGATGGGCTCACAAAGAGCTATGGCATGCTTCCTTATGGGATATGCACGAGTCTCATCACAAGCCGAGGGTTGGAGCATTTGAAAAGAACGATGTGTTTGCAATTATAAACGCAATTCCAGCCATTGCTCTTCTTTCTTTTGGCCTCTTCAATCAAGGCTTCTTTCCCGGCCTTTGTTTCGGTGCCGGTTTGGGAATTACGGTGTTTGGGATGGCTTACATGTTCGTCCACGATGGCCTCGTTCACCGTCGTTTCCCAGTGGGTCCCATAGCCGCTGTCCCCTACTTACGACGTGTCGCTGCTGCCCATCATATTCATCACACAGACAAATTCGACGGAGTTCCATACGGGTTGTTTTTAGGACCCAAGGAACTGGAAGAAGTGGATGGCGAGGAAGAGCTGCAGAAGGAAATTAAAAGGAGGAGAAAGGTTTACAGAAATTAA

Protein sequence

MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDEMVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN
Homology
[Close]
BLAST of PI0023565 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.7e-93
Identity = 166/220 (75.45%), Postives = 189/220 (85.91%), Query Frame = 0 

Query: 65  LKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEMFGTF 124
           L + + EK+ +KK+ER  Y IAA++SSFGITSMA +AVYYRFS Q+ G E  +LEMFGTF
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTF 139

Query: 125 VLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLS 184
            LS+G+AVGMEFWARWAH+ LWHASLW+MHESHHKPR G FE NDVFAI+NA PAI LLS
Sbjct: 140 ALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLS 199

Query: 185 FGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIH 244
           +G FN+G  PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +H
Sbjct: 200 YGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQLH 259

Query: 245 HTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYR 285
           HTDKF+GVPYGLFLGPKELEEV G EEL KEI RR K Y+
Sbjct: 260 HTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYK 299

[Close]
BLAST of PI0023565 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-91
Identity = 168/263 (63.88%), Postives = 206/263 (78.33%), Query Frame = 0 

Query: 28  APSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDEMVKELKMSVE---------EKMGKKKA 87
           +P+ G   RC  +  +    V++  K K++ +   ++++M +E         EK+ +KK+
Sbjct: 41  SPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQISATRLAEKLARKKS 100

Query: 88  EREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEMFGTFVLSIGSAVGMEFWA 147
           ER  Y +AA++SSFGITSMA +AVYYRF  Q+ G E P  EMFGTF LS+G+AVGMEFWA
Sbjct: 101 ERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTFALSVGAAVGMEFWA 160

Query: 148 RWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCF 207
           RWAHK LWHASLW MHESHHKPR G FE NDVFAIINA+PAIALL +G F++G  PGLCF
Sbjct: 161 RWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLDYGFFHKGLIPGLCF 220

Query: 208 GAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFL 267
           GAGLGITVFGMAYMFVHDGLVH+RFPVGP+A VPYLR+VAAAH +HH++KF+GVPYGLFL
Sbjct: 221 GAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLHHSEKFNGVPYGLFL 280

Query: 268 GPKELEEVDGEEELQKEIKRRRK 282
           GPKELEEV G EEL+KE+ RR +
Sbjct: 281 GPKELEEVGGLEELEKEVNRRTR 303

[Close]
BLAST of PI0023565 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 7.7e-91
Identity = 162/222 (72.97%), Postives = 188/222 (84.68%), Query Frame = 0 

Query: 63  KELKMSVE---EKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILE 122
           KE+  S E   +K+ +KK+ER  Y +AA++SSFGITSMA ++VYYRFS Q+ G E P+ E
Sbjct: 95  KEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQMEGGEIPLSE 154

Query: 123 MFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 182
           MFGTF LS+G+AVGMEFWARWAH+ LWHASLW MHESHHKPR G FE ND+FAIINA+PA
Sbjct: 155 MFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPFELNDIFAIINAVPA 214

Query: 183 IALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 242
           IALLS+G F++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA VPY RRVAA
Sbjct: 215 IALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAA 274

Query: 243 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRK 282
           AH +HH+DKF+GVPYGLFLGPKELEEV G + L+ EI RR K
Sbjct: 275 AHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTK 316

[Close]
BLAST of PI0023565 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 2.9e-90
Identity = 168/248 (67.74%), Postives = 195/248 (78.63%), Query Frame = 0 

Query: 37  CFAKQRWRRLMVMKTKKKKSEEDEMVKELKMS-VEEKMGKKKAEREGYFIAAIVSSFGIT 96
           CF  +  ++   M    K        + L  S + +K  KKK+ER  Y IAA++SSFGIT
Sbjct: 49  CFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGIT 108

Query: 97  SMAAIAVYYRFSSQVMGAEFPILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHE 156
           SMA +AVYYRFS Q+ G E  +LEMFGTF LS+G+AVGMEFWARWAH+ LWH SLW+MHE
Sbjct: 109 SMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHE 168

Query: 157 SHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVH 216
           SHHKPR GAFE NDVFAI NA+PAI LL +G  N+G  PGLCFGAGLGIT+FGMAYMFVH
Sbjct: 169 SHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVH 228

Query: 217 DGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKE 276
           DGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+EEV G+EEL+KE
Sbjct: 229 DGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKE 288

Query: 277 IKRRRKVY 284
           I RR K+Y
Sbjct: 289 ISRRIKLY 296

[Close]
BLAST of PI0023565 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 326.2 bits (835), Expect = 3.6e-88
Identity = 157/219 (71.69%), Postives = 182/219 (83.11%), Query Frame = 0 

Query: 63  KELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEMFG 122
           K L + + EK  +KK+ER  Y +AA++SS GITSMA I+VYYRFS Q+ G E P  EMF 
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFC 151

Query: 123 TFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIAL 182
           TF L+ G+A+GME+WARWAH+ LWHASLW MHESHH+PR G FE ND+FAIINA+PAIAL
Sbjct: 152 TFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIAL 211

Query: 183 LSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHH 242
           LSFG  ++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA VPY +RVAAAH 
Sbjct: 212 LSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQ 271

Query: 243 IHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRK 282
           +HH+DKFDGVPYGLFLGPKELEEV   EEL+KE+ RR K
Sbjct: 272 LHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIK 310

[Close]
BLAST of PI0023565 vs. ExPASy TrEMBL
Match: A0A0A0KNU9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152820 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.3e-149
Identity = 276/289 (95.50%), Postives = 279/289 (96.54%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRP-AGAPSPGFPWRCFAKQRW--RRLMVMKTKKKKSE 60
           M TVISAVATAGPYRYDY RLFQRP AGAPSPGFPWRCFAKQRW  RRLMV+K KKKKSE
Sbjct: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVM-GAEFP 120
           EDEMVKELKMSVEEKMGKKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M G EFP
Sbjct: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 ILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           +LEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI RRRKVYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI-RRRKVYRN 288

[Close]
BLAST of PI0023565 vs. ExPASy TrEMBL
Match: A0A1S3AUA9 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.4e-149
Identity = 272/286 (95.10%), Postives = 275/286 (96.15%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDE 60
           MATVISAVATAG YRYDY RLFQRPAGAPS GFPWRCFAKQRWRRLMVMK KKKKSEEDE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPSRGFPWRCFAKQRWRRLMVMKMKKKKSEEDE 60

Query: 61  MVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVM-GAEFPILE 120
           MVK+LKMSVEEKM KKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M G EFPILE
Sbjct: 61  MVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPILE 120

Query: 121 MFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180
           MFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA
Sbjct: 121 MFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180

Query: 181 IALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI RRR VYRN
Sbjct: 241 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI-RRRNVYRN 285

[Close]
BLAST of PI0023565 vs. ExPASy TrEMBL
Match: A0A1S4DS92 (beta-carotene hydroxylase 2, chloroplastic-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 4.8e-136
Identity = 254/285 (89.12%), Postives = 256/285 (89.82%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDE 60
           MATVISAVATAG YRYDY RLFQRPAGAPS GFPWRCFAKQRWRRLMVMK KKKKSEEDE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPSRGFPWRCFAKQRWRRLMVMKMKKKKSEEDE 60

Query: 61  MVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEM 120
           MVK+LKMSVEEKM KKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M         
Sbjct: 61  MVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIM--------- 120

Query: 121 FGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAI 180
                      VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAI
Sbjct: 121 ----------EVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAI 180

Query: 181 ALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAA 240
           ALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAA
Sbjct: 181 ALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAA 240

Query: 241 HHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           HHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI RRR VYRN
Sbjct: 241 HHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI-RRRNVYRN 265

[Close]
BLAST of PI0023565 vs. ExPASy TrEMBL
Match: A0A1S4DT22 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.2e-132
Identity = 253/286 (88.46%), Postives = 256/286 (89.51%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDE 60
           MATVISAVATAG YRYDY RLFQRPAGAPS GFPWRCFAKQRWRRLMVMK KKKKSEEDE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPSRGFPWRCFAKQRWRRLMVMKMKKKKSEEDE 60

Query: 61  MVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVM-GAEFPILE 120
           MVK+LKMSVEEKM KKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M G EFPILE
Sbjct: 61  MVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPILE 120

Query: 121 MFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180
           MFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA
Sbjct: 121 MFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180

Query: 181 IALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           AHH                   ELEEVDGEEELQKEI RRR VYRN
Sbjct: 241 AHH-------------------ELEEVDGEEELQKEI-RRRNVYRN 266

[Close]
BLAST of PI0023565 vs. ExPASy TrEMBL
Match: A0A6J1I1U3 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.3e-122
Identity = 229/287 (79.79%), Postives = 251/287 (87.46%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPG--FPWRCFAKQRWRRLMVMKTKKKKSEE 60
           MAT ISAVATA P    YRR+ QRPA AP+P    PWRCFAKQ  RRL  +K KK    E
Sbjct: 1   MATGISAVATAAP----YRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVK-KKCGVSE 60

Query: 61  DEMVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPIL 120
            EMV++LK+ VEEKMG+KKAER+ Y +AAIVSSFGITSMAAIAVYYRFS Q+ G +FP+L
Sbjct: 61  VEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVL 120

Query: 121 EMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIP 180
           EMFGTF LS+G+AVGMEFWARWAHK+LWH +LW+MH+SHH+ R GAFE NDVFAIINA+P
Sbjct: 121 EMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVP 180

Query: 181 AIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240
           AIALLSFG FNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA
Sbjct: 181 AIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240

Query: 241 AAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           AAHHIHHTDKFDGVPYGLFLGPKELE+VDGEEELQ EIK+R KVYRN
Sbjct: 241 AAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 282

[Close]
BLAST of PI0023565 vs. NCBI nr
Match: XP_004143973.1 (beta-carotene 3-hydroxylase 1, chloroplastic [Cucumis sativus] >KGN50067.1 hypothetical protein Csa_000162 [Cucumis sativus])

HSP 1 Score: 538.9 bits (1387), Expect = 2.7e-149
Identity = 276/289 (95.50%), Postives = 279/289 (96.54%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRP-AGAPSPGFPWRCFAKQRW--RRLMVMKTKKKKSE 60
           M TVISAVATAGPYRYDY RLFQRP AGAPSPGFPWRCFAKQRW  RRLMV+K KKKKSE
Sbjct: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVM-GAEFP 120
           EDEMVKELKMSVEEKMGKKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M G EFP
Sbjct: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 ILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           +LEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI RRRKVYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI-RRRKVYRN 288

[Close]
BLAST of PI0023565 vs. NCBI nr
Match: XP_008437325.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 272/286 (95.10%), Postives = 275/286 (96.15%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDE 60
           MATVISAVATAG YRYDY RLFQRPAGAPS GFPWRCFAKQRWRRLMVMK KKKKSEEDE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPSRGFPWRCFAKQRWRRLMVMKMKKKKSEEDE 60

Query: 61  MVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVM-GAEFPILE 120
           MVK+LKMSVEEKM KKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M G EFPILE
Sbjct: 61  MVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPILE 120

Query: 121 MFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180
           MFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA
Sbjct: 121 MFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180

Query: 181 IALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI RRR VYRN
Sbjct: 241 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI-RRRNVYRN 285

[Close]
BLAST of PI0023565 vs. NCBI nr
Match: XP_016898856.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X3 [Cucumis melo])

HSP 1 Score: 493.8 bits (1270), Expect = 9.9e-136
Identity = 254/285 (89.12%), Postives = 256/285 (89.82%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDE 60
           MATVISAVATAG YRYDY RLFQRPAGAPS GFPWRCFAKQRWRRLMVMK KKKKSEEDE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPSRGFPWRCFAKQRWRRLMVMKMKKKKSEEDE 60

Query: 61  MVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEM 120
           MVK+LKMSVEEKM KKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M         
Sbjct: 61  MVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIM--------- 120

Query: 121 FGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAI 180
                      VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAI
Sbjct: 121 ----------EVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAI 180

Query: 181 ALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAA 240
           ALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAA
Sbjct: 181 ALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAA 240

Query: 241 HHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           HHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI RRR VYRN
Sbjct: 241 HHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI-RRRNVYRN 265

[Close]
BLAST of PI0023565 vs. NCBI nr
Match: XP_016898855.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 481.1 bits (1237), Expect = 6.6e-132
Identity = 253/286 (88.46%), Postives = 256/286 (89.51%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSPGFPWRCFAKQRWRRLMVMKTKKKKSEEDE 60
           MATVISAVATAG YRYDY RLFQRPAGAPS GFPWRCFAKQRWRRLMVMK KKKKSEEDE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPSRGFPWRCFAKQRWRRLMVMKMKKKKSEEDE 60

Query: 61  MVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVM-GAEFPILE 120
           MVK+LKMSVEEKM KKKAEREGY IAAIVSSFGITSMAAIAVYYRFSSQ+M G EFPILE
Sbjct: 61  MVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPILE 120

Query: 121 MFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180
           MFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA
Sbjct: 121 MFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPA 180

Query: 181 IALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           AHH                   ELEEVDGEEELQKEI RRR VYRN
Sbjct: 241 AHH-------------------ELEEVDGEEELQKEI-RRRNVYRN 266

[Close]
BLAST of PI0023565 vs. NCBI nr
Match: XP_038907152.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 475.7 bits (1223), Expect = 2.8e-130
Identity = 242/291 (83.16%), Postives = 258/291 (88.66%), Query Frame = 0 

Query: 1   MATVISAVATAGPYRYDYRRLFQRPAGAPSP------GFPWRCFAKQRWRRLMVMKTKKK 60
           MATVI+AVATA P+    +R F RPAGAP P       FPWRCFA+QR RRL+ M  KKK
Sbjct: 1   MATVIAAVATAAPFN---QRFFHRPAGAPPPRLGFSNSFPWRCFAEQRSRRLLAM--KKK 60

Query: 61  KSEEDEMVKELKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAE 120
           KSEEDEMVKELKMSV++KMG+KKAER+ Y +AAIVSS G TSMAAIAVYYRFS Q+ G E
Sbjct: 61  KSEEDEMVKELKMSVDKKMGRKKAERDAYLVAAIVSSIGFTSMAAIAVYYRFSWQMEGRE 120

Query: 121 FPILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAII 180
            PI EMFGTF LSIG+AVGMEFWARWAHKELWH SLWDMHESHH+PRVGAFE NDVFAII
Sbjct: 121 IPIWEMFGTFALSIGTAVGMEFWARWAHKELWHGSLWDMHESHHRPRVGAFELNDVFAII 180

Query: 181 NAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYL 240
           NA+PAI+LLSFG FNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFP+GPIAAVPYL
Sbjct: 181 NAVPAISLLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPLGPIAAVPYL 240

Query: 241 RRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYRN 286
           RRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRR KVYRN
Sbjct: 241 RRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRIKVYRN 286

[Close]
BLAST of PI0023565 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 344.0 bits (881), Expect = 1.2e-94
Identity = 166/220 (75.45%), Postives = 189/220 (85.91%), Query Frame = 0 

Query: 65  LKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEMFGTF 124
           L + + EK+ +KK+ER  Y IAA++SSFGITSMA +AVYYRFS Q+ G E  +LEMFGTF
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTF 139

Query: 125 VLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLS 184
            LS+G+AVGMEFWARWAH+ LWHASLW+MHESHHKPR G FE NDVFAI+NA PAI LLS
Sbjct: 140 ALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLS 199

Query: 185 FGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIH 244
           +G FN+G  PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +H
Sbjct: 200 YGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQLH 259

Query: 245 HTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRRKVYR 285
           HTDKF+GVPYGLFLGPKELEEV G EEL KEI RR K Y+
Sbjct: 260 HTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYK 299

[Close]
BLAST of PI0023565 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 333.2 bits (853), Expect = 2.1e-91
Identity = 168/248 (67.74%), Postives = 195/248 (78.63%), Query Frame = 0 

Query: 37  CFAKQRWRRLMVMKTKKKKSEEDEMVKELKMS-VEEKMGKKKAEREGYFIAAIVSSFGIT 96
           CF  +  ++   M    K        + L  S + +K  KKK+ER  Y IAA++SSFGIT
Sbjct: 49  CFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGIT 108

Query: 97  SMAAIAVYYRFSSQVMGAEFPILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHE 156
           SMA +AVYYRFS Q+ G E  +LEMFGTF LS+G+AVGMEFWARWAH+ LWH SLW+MHE
Sbjct: 109 SMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHE 168

Query: 157 SHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVH 216
           SHHKPR GAFE NDVFAI NA+PAI LL +G  N+G  PGLCFGAGLGIT+FGMAYMFVH
Sbjct: 169 SHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVH 228

Query: 217 DGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKE 276
           DGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+EEV G+EEL+KE
Sbjct: 229 DGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKE 288

Query: 277 IKRRRKVY 284
           I RR K+Y
Sbjct: 289 ISRRIKLY 296

[Close]
BLAST of PI0023565 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 201.1 bits (510), Expect = 1.2e-51
Identity = 96/136 (70.59%), Postives = 113/136 (83.09%), Query Frame = 0 

Query: 65  LKMSVEEKMGKKKAEREGYFIAAIVSSFGITSMAAIAVYYRFSSQVMGAEFPILEMFGTF 124
           L + + EK+ +KK+ER  Y IAA++SSFGITSMA +AVYYRFS Q+ G E  +LEMFGTF
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTF 139

Query: 125 VLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLS 184
            LS+G+AVGMEFWARWAH+ LWHASLW+MHESHHKPR G FE NDVFAI+NA PAI LLS
Sbjct: 140 ALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLS 199

Query: 185 FGLFNQGFFPGLCFGA 201
           +G FN+G  PGLCFGA
Sbjct: 200 YGFFNKGLVPGLCFGA 215

[Close]
BLAST of PI0023565 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 185.3 bits (469), Expect = 7.0e-47
Identity = 115/248 (46.37%), Postives = 137/248 (55.24%), Query Frame = 0 

Query: 37  CFAKQRWRRLMVMKTKKKKSEEDEMVKELKMS-VEEKMGKKKAEREGYFIAAIVSSFGIT 96
           CF  +  ++   M    K        + L  S + +K  KKK+ER  Y IAA++SSFGIT
Sbjct: 49  CFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGIT 108

Query: 97  SMAAIAVYYRFSSQVMGAEFPILEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHE 156
           SMA +AVYYRFS Q+ G E  +LEMFGTF LS+G+                         
Sbjct: 109 SMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGA------------------------- 168

Query: 157 SHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVH 216
                                                       AGLGIT+FGMAYMFVH
Sbjct: 169 --------------------------------------------AGLGITMFGMAYMFVH 227

Query: 217 DGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKE 276
           DGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+EEV G+EEL+KE
Sbjct: 229 DGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKE 227

Query: 277 IKRRRKVY 284
           I RR K+Y
Sbjct: 289 ISRRIKLY 227

The following BLAST results are available for this feature:
BLAST of PI0023565 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SZZ81.7e-9375.45Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498141.2e-9163.88Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=... [more]
B3SGL07.7e-9172.97Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=... [more]
Q9LTG02.9e-9067.74Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498153.6e-8871.69Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=... [more]
BLAST of PI0023565 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KNU91.3e-14995.50Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3AUA98.4e-14995.10beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=36... [more]
A0A1S4DS924.8e-13689.12beta-carotene hydroxylase 2, chloroplastic-like isoform X3 OS=Cucumis melo OX=36... [more]
A0A1S4DT223.2e-13288.46beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=36... [more]
A0A6J1I1U31.3e-12279.79beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima... [more]
BLAST of PI0023565 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004143973.12.7e-14995.50beta-carotene 3-hydroxylase 1, chloroplastic [Cucumis sativus] >KGN50067.1 hypot... [more]
XP_008437325.11.7e-14895.10PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucumis m... [more]
XP_016898856.19.9e-13689.12PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X3 [Cucumis m... [more]
XP_016898855.16.6e-13288.46PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucumis m... [more]
XP_038907152.12.8e-13083.16beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Benincasa hispida][more]
BLAST of PI0023565 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G25700.11.2e-9475.45beta-hydroxylase 1 [more]
AT5G52570.12.1e-9167.74beta-carotene hydroxylase 2 [more]
AT4G25700.21.2e-5170.59beta-hydroxylase 1 [more]
AT5G52570.27.0e-4746.37beta-carotene hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 131..257
e-value: 9.4E-13
score: 48.7
NoneNo IPR availablePANTHERPTHR31899:SF14HYDROXYLASE, PUTATIVE, EXPRESSED-RELATEDcoord: 43..281
IPR045019Beta-carotene hydroxylase-likePANTHERPTHR31899BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTICcoord: 43..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0023565.1PI0023565.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity