PI0023313 (gene) Melon (PI 482460) v1

Overview


Name	PI0023313
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Location	chr10: 20902194 .. 20902979 (-)
RNA-Seq Expression	PI0023313
Synteny	PI0023313

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCAACAGCATTCCTCTTCTCCCCATTTTCTTCTCTTTTCCTCTTATCTCTTTCTTCCCTAACCTTCCTTTCCATTCTCTTCCTCGTTTCCGGCGAATTCCTCGGCCACCATTTACAATACTCCAAATTCTGGACCCCTAATTCCAAATCCCACCTCCCTTCACGTTCCGCCATGCTCCTCTGTTACCTCCCTGCTTTCTCCCTCTCATCTCTCTCTCTCTTCCTCTTCCCTCACCATTTCCTTCTCTCCTCCAGAATCTTCCTCATCATTTCCCTCCTCCTCTTCCATTTCTTCAAACGCATCTCCGAGGTCCTGTTCGTTCACAAATACAGTAACAAAATGGCTCTCACCGACGCCACTATCACCTCCCTTTCTTACCTTCTCTTCACCGGCACCACGATCTACGCTCAGATTGTTTCACAGGATCTGGCGGAACCGGAGATTGATCTGAAGGCGGCGGGATTAGCGATGTATGGGATTGGAATTACTGGTAATTTCTATCACCATGTTTTACTGTCGAGATTGAGAAGTGGAGGCGATCGAGAGTATAGGATTCCGAGAGGAGGATTGTTTGAAGTGATTGTGTGTCCTCATTATCTATTTGAGATTGTGGAGTTTTTGGGAATTGCATTTGTTTCACAGACGTTGTTTGCGACGGTGTTCGTGGTGGGATCGGCGTTGTTCTTGATCGGAAGAAGCTATGCTACAAGGAAGTGGTACCTTTCTAAATTTGAAGATTTTCCTAGCCATGTTAAGGCTATTCTCCCCTTTGTCTTTTGA

mRNA sequence

ATGGCGGCAACAGCATTCCTCTTCTCCCCATTTTCTTCTCTTTTCCTCTTATCTCTTTCTTCCCTAACCTTCCTTTCCATTCTCTTCCTCGTTTCCGGCGAATTCCTCGGCCACCATTTACAATACTCCAAATTCTGGACCCCTAATTCCAAATCCCACCTCCCTTCACGTTCCGCCATGCTCCTCTGTTACCTCCCTGCTTTCTCCCTCTCATCTCTCTCTCTCTTCCTCTTCCCTCACCATTTCCTTCTCTCCTCCAGAATCTTCCTCATCATTTCCCTCCTCCTCTTCCATTTCTTCAAACGCATCTCCGAGGTCCTGTTCGTTCACAAATACAGTAACAAAATGGCTCTCACCGACGCCACTATCACCTCCCTTTCTTACCTTCTCTTCACCGGCACCACGATCTACGCTCAGATTGTTTCACAGGATCTGGCGGAACCGGAGATTGATCTGAAGGCGGCGGGATTAGCGATGTATGGGATTGGAATTACTGGTAATTTCTATCACCATGTTTTACTGTCGAGATTGAGAAGTGGAGGCGATCGAGAGTATAGGATTCCGAGAGGAGGATTGTTTGAAGTGATTGTGTGTCCTCATTATCTATTTGAGATTGTGGAGTTTTTGGGAATTGCATTTGTTTCACAGACGTTGTTTGCGACGGTGTTCGTGGTGGGATCGGCGTTGTTCTTGATCGGAAGAAGCTATGCTACAAGGAAGTGGTACCTTTCTAAATTTGAAGATTTTCCTAGCCATGTTAAGGCTATTCTCCCCTTTGTCTTTTGA

Coding sequence (CDS)

ATGGCGGCAACAGCATTCCTCTTCTCCCCATTTTCTTCTCTTTTCCTCTTATCTCTTTCTTCCCTAACCTTCCTTTCCATTCTCTTCCTCGTTTCCGGCGAATTCCTCGGCCACCATTTACAATACTCCAAATTCTGGACCCCTAATTCCAAATCCCACCTCCCTTCACGTTCCGCCATGCTCCTCTGTTACCTCCCTGCTTTCTCCCTCTCATCTCTCTCTCTCTTCCTCTTCCCTCACCATTTCCTTCTCTCCTCCAGAATCTTCCTCATCATTTCCCTCCTCCTCTTCCATTTCTTCAAACGCATCTCCGAGGTCCTGTTCGTTCACAAATACAGTAACAAAATGGCTCTCACCGACGCCACTATCACCTCCCTTTCTTACCTTCTCTTCACCGGCACCACGATCTACGCTCAGATTGTTTCACAGGATCTGGCGGAACCGGAGATTGATCTGAAGGCGGCGGGATTAGCGATGTATGGGATTGGAATTACTGGTAATTTCTATCACCATGTTTTACTGTCGAGATTGAGAAGTGGAGGCGATCGAGAGTATAGGATTCCGAGAGGAGGATTGTTTGAAGTGATTGTGTGTCCTCATTATCTATTTGAGATTGTGGAGTTTTTGGGAATTGCATTTGTTTCACAGACGTTGTTTGCGACGGTGTTCGTGGTGGGATCGGCGTTGTTCTTGATCGGAAGAAGCTATGCTACAAGGAAGTGGTACCTTTCTAAATTTGAAGATTTTCCTAGCCATGTTAAGGCTATTCTCCCCTTTGTCTTTTGA

Protein sequence

MAATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAMLLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKWYLSKFEDFPSHVKAILPFVF

Homology

[Close]

BLAST of PI0023313 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.7e-15
Identity = 63/228 (27.63%), Postives = 109/228 (47.81%), Query Frame = 0


Query: 39  HLQYSKFWTPNSKSHLPSRSAMLLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFH 98
           +L Y +  +P+ +  +P+R+A ++  LP+ +L            L S+   +++++ L H
Sbjct: 39  NLAYGRHASPSFRVRVPARAAWVVQELPSLALPLYQYASESAPRLRSAPNCILLAMFLVH 98

Query: 99  FFKRISEVLFVHKYSNKMALTDATI-----TSLSYLLFTGTTIYAQIVSQDLAEPEIDLK 158
           +  R     F+ +    M L   T+     T   YL     + +A      + +P     
Sbjct: 99  YGHRCLIYPFLMRGGKPMPLLACTMAIMFCTFNGYLQSRYLSHWAVYADDWVTDPRF--- 158

Query: 159 AAGLAMYGIGITGNFYHHVLLSRLRSGGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAF 218
             G  ++  G+  N +   +L  LR  GD  Y+IPRGGLFE +   +Y  EI+E+ G A 
Sbjct: 159 LIGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYAL 218

Query: 219 VSQTLFATVFVVGSALFLIGRSYATRKWYLSKFEDFPSHVKAILPFVF 262
            S ++    F   +  FL GR+    +WYL KFE++P   K ++PF+F
Sbjct: 219 ASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of PI0023313 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 8.2e-15
Identity = 82/273 (30.04%), Postives = 136/273 (49.82%), Query Frame = 0


Query: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAM 60
           MA+   LF  +  L L  ++  T++S+ FL           Y K    N     P+ S  
Sbjct: 1   MASDQTLFH-YCLLTLYIIALPTWISLYFL--------QAPYGKH---NRPGWGPTISPP 60

Query: 61  LLCYLPAFSLSSLSLFLFP--HHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMAL 120
           L  +L       L+ FLFP   HF  + + FL+IS  LFH+F R   VL+  + +     
Sbjct: 61  LAWFLMESPTLWLTFFLFPSGQHF-YNPKSFLLISPFLFHYFNR--TVLYPLRLARNTTQ 120

Query: 121 TDATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLK---------AAGLAMYGIGITGNFY 180
           T     S++++ F    +   + ++ ++  + D +          AGL ++ +G+  N  
Sbjct: 121 TRGFPVSVAFMAFGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVR 180

Query: 181 HHVLLSRLRSGGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSAL 240
              +L  L+  GD  Y+IPRGGLFE++ CP+Y  EI+E+ G A ++ +     F + +  
Sbjct: 181 ADKVLVGLKKQGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCA 240

Query: 241 FLIGRSYATRKWYLSKF-EDFPSHVKAILPFVF 262
            L+ R+ ATR WYL KF +D+P   KA++PF++
Sbjct: 241 NLMPRARATRLWYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of PI0023313 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.4e-14
Identity = 40/105 (38.10%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 156 GLAMYGIGITGNFYHHVLLSRLRSGGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVS 215
           G A++ +G+  N +   +L  LR  G+  Y+IPRGGLFE +   +Y  E+VE+ G A  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 216 QTLFATVFVVGSALFLIGRSYATRKWYLSKFEDFPSHVKAILPFV 261
            +L   VF + +   L+ R+    +WY  KFED+P   K ++PFV
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of PI0023313 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.1e-14
Identity = 63/225 (28.00%), Postives = 105/225 (46.67%), Query Frame = 0


Query: 42  YSKFWTPNSKSHLPSRSAMLLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFK 101
           Y +   P+ +  +P+R+A ++  LP+ +L            L S+   +++++ L H+  
Sbjct: 38  YGRHALPSHRLRVPARAAWVVQELPSLALPLYQYASESAPRLRSAPNCILLAMFLVHYGH 97

Query: 102 RISEVLFVHKYSNKMALTDATI-----TSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAG 161
           R     F+ +    M L   T+     T   YL     +  A      + +P       G
Sbjct: 98  RCLIYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDWVTDPRF---LIG 157

Query: 162 LAMYGIGITGNFYHHVLLSRLRSGGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQ 221
             ++  G+  N +   +L  LR  GD  Y+IPRGGLFE +   +Y  EI+E+ G A  S 
Sbjct: 158 FGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALASW 217

Query: 222 TLFATVFVVGSALFLIGRSYATRKWYLSKFEDFPSHVKAILPFVF 262
           ++    F   +  FL GR+    +WYL KFE++P   K I+PF+F
Sbjct: 218 SVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of PI0023313 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.5e-13
Identity = 58/199 (29.15%), Postives = 100/199 (50.25%), Query Frame = 0


Query: 75  LFLFPHHFLLSSRIF-----------LIISLLLFHFFKRISEVLFVHKYSNKMALTDATI 134
           LF++P  + LS+ I+           + +     H+ KRI E +FVH++S+         
Sbjct: 102 LFVYPIFYFLSNLIYGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLF 161

Query: 135 TSLSYLLFTGTTIYAQIVSQDL-AEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGGD 194
            + SY  +  T + +  V+  L  E  I+    GL ++ IG   N+  H+ L  LR  G 
Sbjct: 162 KNCSY-YWGCTAMVSYFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGS 221

Query: 195 REYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKWY 254
            E +IPRG LFE + CP+Y  EI+ ++G + ++QTL + +F +  A  +   +    + Y
Sbjct: 222 TERKIPRGLLFEFVSCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRY 281

Query: 255 LSKFED-FPSHVKAILPFV 261
             +F D +P   K ++PF+
Sbjct: 282 RKEFGDKYPKSRKILIPFL 299

[Close]

BLAST of PI0023313 vs. ExPASy TrEMBL
Match: A0A1S3BFS4 (steroid 5-alpha-reductase DET2-like OS=Cucumis melo OX=3656 GN=LOC103489358 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.3e-127
Identity = 246/261 (94.25%), Postives = 251/261 (96.17%), Query Frame = 0


Query: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAM 60
           MAATAFLF PFSSLFLLSLSSLTFLSILFLVSGE LGH L+YSKFWTPNSK HLPSRSAM
Sbjct: 1   MAATAFLFYPFSSLFLLSLSSLTFLSILFLVSGELLGHPLRYSKFWTPNSKFHLPSRSAM 60

Query: 61  LLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120
           LLCYLPAFSLSSLSLFL PHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD
Sbjct: 61  LLCYLPAFSLSSLSLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120

Query: 121 ATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 180
           A ITSL+YLLFTG TIYAQIVS DL EPEIDLKAAGLA+YGIGITGNFYHHVLLSRLRSG
Sbjct: 121 AIITSLAYLLFTGATIYAQIVSLDLVEPEIDLKAAGLALYGIGITGNFYHHVLLSRLRSG 180

Query: 181 GDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRK 240
           GDREYRIPRGGLF+VIVCPHYLFEIVEFLGIAFVSQTLFA VFVVGSALFLIGRSYATRK
Sbjct: 181 GDREYRIPRGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFAVVFVVGSALFLIGRSYATRK 240

Query: 241 WYLSKFEDFPSHVKAILPFVF 262
           WYLSKFEDFP+HVKAILPFVF
Sbjct: 241 WYLSKFEDFPTHVKAILPFVF 261

[Close]

BLAST of PI0023313 vs. ExPASy TrEMBL
Match: A0A0A0KUP2 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612860 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 3.8e-124
Identity = 240/261 (91.95%), Postives = 248/261 (95.02%), Query Frame = 0


Query: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAM 60
           MAATAFLFSPFSSLFLLSLSSLTFLSIL LVSGEFLGH L+YSKFWTPNSK HLPSRSAM
Sbjct: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILLLVSGEFLGHPLRYSKFWTPNSKPHLPSRSAM 60

Query: 61  LLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120
           LLCYLPAF     SLFL PHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD
Sbjct: 61  LLCYLPAF-----SLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120

Query: 121 ATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 180
           A +TSLSYLLF+GTTIYAQIV+ DLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG
Sbjct: 121 AIMTSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 180

Query: 181 GDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRK 240
           GDREY+IP+GGLF+VIVCPHYLFEIVEFLGIAFVSQTLF  VFVVGSALFLIGRSYATRK
Sbjct: 181 GDREYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRSYATRK 240

Query: 241 WYLSKFEDFPSHVKAILPFVF 262
           WYLSKFEDFPSH+KAILPFVF
Sbjct: 241 WYLSKFEDFPSHIKAILPFVF 256

[Close]

BLAST of PI0023313 vs. ExPASy TrEMBL
Match: A0A6J1JAL0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483217 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 1.0e-100
Identity = 198/260 (76.15%), Postives = 227/260 (87.31%), Query Frame = 0


Query: 6   FLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTP---NSKSHLPSRSAMLL 65
           FLFSPFSS F+L+LSSL  LS L L + EFLG HLQYSKFW+P   ++K HLPSRSAMLL
Sbjct: 4   FLFSPFSSPFILALSSLIMLSTLVLATSEFLGRHLQYSKFWSPKNSSNKIHLPSRSAMLL 63

Query: 66  CYLPAFSLSSLSLF-LFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDA 125
           CY+PAF+++S+SLF L P +   S+RIFLI SLLL HFFKRI EV+FVHKYSNKM L+DA
Sbjct: 64  CYVPAFAVASISLFLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYSNKMGLSDA 123

Query: 126 TITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGG 185
            I S++Y+L TG+TIY+Q+V+QDLAEP+IDL+AAGLAMY IGITGN YHHVLLS+LR  G
Sbjct: 124 IIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLSKLRKEG 183

Query: 186 DREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKW 245
           DREYRIPRGG+FEVIVCPHYLFE+VEFLGIAFVSQTLFA VF +GSALFLIGRSYATR W
Sbjct: 184 DREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRSYATRNW 243

Query: 246 YLSKFEDFPSHVKAILPFVF 262
           Y+SKFEDFP HVKAILPFVF
Sbjct: 244 YVSKFEDFPRHVKAILPFVF 263

[Close]

BLAST of PI0023313 vs. ExPASy TrEMBL
Match: A0A6J1GYE2 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458608 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 4.0e-97
Identity = 193/261 (73.95%), Postives = 223/261 (85.44%), Query Frame = 0


Query: 6   FLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTP---NSKSHLPSRSAMLL 65
           FLFSPFSS FLL+LSSLT LSIL L + EFLG HLQYSKFW+P   ++K HLPSRSAMLL
Sbjct: 4   FLFSPFSSPFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLL 63

Query: 66  CYLPAFSLSSLSLF--LFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 125
           CY+PAF+++S+SLF  L P +   S+RIFLI SLLL HFFKRI EV+FVHKY NKM L++
Sbjct: 64  CYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYGNKMGLSE 123

Query: 126 ATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 185
           A   S++Y+L T +TIY+Q+VSQ+L EP+IDL+AAGLAMY IGITGN YHHVLL++LR  
Sbjct: 124 AIRVSITYVLLTASTIYSQMVSQNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKE 183

Query: 186 GDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRK 245
           GDREYRIPRGG+FEVIVCPHYLFE+VEFLG+AFVSQTLF  VF  GSALFLIGRSYATR 
Sbjct: 184 GDREYRIPRGGMFEVIVCPHYLFEMVEFLGLAFVSQTLFPMVFAFGSALFLIGRSYATRN 243

Query: 246 WYLSKFEDFPSHVKAILPFVF 262
           WY+SKFEDFP HVKAILPFVF
Sbjct: 244 WYVSKFEDFPRHVKAILPFVF 264

[Close]

BLAST of PI0023313 vs. ExPASy TrEMBL
Match: A0A6J1DCA9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018767 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 7.3e-91
Identity = 177/250 (70.80%), Postives = 207/250 (82.80%), Query Frame = 0


Query: 15  FLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNS---KSHLPSRSAMLLCYLPAFSLS 74
           FLL+LSS+TF+SIL L + EFLG HLQYSKFW+P +   K HL SRSAMLLCY PAF+L+
Sbjct: 37  FLLALSSITFVSILILAAAEFLGKHLQYSKFWSPKNSAHKIHLHSRSAMLLCYAPAFALT 96

Query: 75  SLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDATITSLSYLLF 134
            +  F FP H     RIFLI SLLL HF KRI EV+FVHK+SNKMAL DA +TS+ Y+LF
Sbjct: 97  LMISFFFPRHIHPDPRIFLIKSLLLLHFSKRIFEVVFVHKHSNKMALNDAILTSICYILF 156

Query: 135 TGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGGDREYRIPRGG 194
           +  TIYAQ+VSQDLAEPE+DL  AGLA+Y IGI GNFYHH LLS+LR  G+REYRIP+GG
Sbjct: 157 SAATIYAQMVSQDLAEPEMDLVPAGLALYSIGIAGNFYHHFLLSKLRREGEREYRIPKGG 216

Query: 195 LFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKWYLSKFEDFPS 254
           LFE +VCPHYLFEI+EFLGI+FVSQTLF+ VF  GSALFLIGRS+ATR WY+SKFEDFP 
Sbjct: 217 LFEAVVCPHYLFEIIEFLGISFVSQTLFSFVFTFGSALFLIGRSHATRNWYVSKFEDFPP 276

Query: 255 HVKAILPFVF 262
           H+KAI+PF+F
Sbjct: 277 HLKAIVPFLF 286

[Close]

BLAST of PI0023313 vs. NCBI nr
Match: XP_008446721.1 (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo])

HSP 1 Score: 465.7 bits (1197), Expect = 2.6e-127
Identity = 246/261 (94.25%), Postives = 251/261 (96.17%), Query Frame = 0


Query: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAM 60
           MAATAFLF PFSSLFLLSLSSLTFLSILFLVSGE LGH L+YSKFWTPNSK HLPSRSAM
Sbjct: 1   MAATAFLFYPFSSLFLLSLSSLTFLSILFLVSGELLGHPLRYSKFWTPNSKFHLPSRSAM 60

Query: 61  LLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120
           LLCYLPAFSLSSLSLFL PHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD
Sbjct: 61  LLCYLPAFSLSSLSLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120

Query: 121 ATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 180
           A ITSL+YLLFTG TIYAQIVS DL EPEIDLKAAGLA+YGIGITGNFYHHVLLSRLRSG
Sbjct: 121 AIITSLAYLLFTGATIYAQIVSLDLVEPEIDLKAAGLALYGIGITGNFYHHVLLSRLRSG 180

Query: 181 GDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRK 240
           GDREYRIPRGGLF+VIVCPHYLFEIVEFLGIAFVSQTLFA VFVVGSALFLIGRSYATRK
Sbjct: 181 GDREYRIPRGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFAVVFVVGSALFLIGRSYATRK 240

Query: 241 WYLSKFEDFPSHVKAILPFVF 262
           WYLSKFEDFP+HVKAILPFVF
Sbjct: 241 WYLSKFEDFPTHVKAILPFVF 261

[Close]

BLAST of PI0023313 vs. NCBI nr
Match: XP_004135372.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus])

HSP 1 Score: 454.1 bits (1167), Expect = 7.9e-124
Identity = 240/261 (91.95%), Postives = 248/261 (95.02%), Query Frame = 0


Query: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAM 60
           MAATAFLFSPFSSLFLLSLSSLTFLSIL LVSGEFLGH L+YSKFWTPNSK HLPSRSAM
Sbjct: 1   MAATAFLFSPFSSLFLLSLSSLTFLSILLLVSGEFLGHPLRYSKFWTPNSKPHLPSRSAM 60

Query: 61  LLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120
           LLCYLPAF     SLFL PHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD
Sbjct: 61  LLCYLPAF-----SLFLVPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTD 120

Query: 121 ATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 180
           A +TSLSYLLF+GTTIYAQIV+ DLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG
Sbjct: 121 AIMTSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSG 180

Query: 181 GDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRK 240
           GDREY+IP+GGLF+VIVCPHYLFEIVEFLGIAFVSQTLF  VFVVGSALFLIGRSYATRK
Sbjct: 181 GDREYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRSYATRK 240

Query: 241 WYLSKFEDFPSHVKAILPFVF 262
           WYLSKFEDFPSH+KAILPFVF
Sbjct: 241 WYLSKFEDFPSHIKAILPFVF 256

[Close]

BLAST of PI0023313 vs. NCBI nr
Match: XP_038892723.1 (steroid 5-alpha-reductase DET2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 401.0 bits (1029), Expect = 8.0e-108
Identity = 213/260 (81.92%), Postives = 224/260 (86.15%), Query Frame = 0


Query: 3   ATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAMLL 62
           A+ FLFSPFSS FLL+LS LTFL  L L S EFLG HL+YSKFWTPNSK HLPSRSAMLL
Sbjct: 2   ASTFLFSPFSSSFLLALSFLTFLVTLLLASAEFLGRHLRYSKFWTPNSKFHLPSRSAMLL 61

Query: 63  CYLPAFSLSSL-SLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDA 122
           CY PA SLSS+ SLFLFPHHF   SRIFLI SL   HFFKRI EVLFVHKYSNKMAL DA
Sbjct: 62  CYFPALSLSSISSLFLFPHHFPHFSRIFLINSLFFIHFFKRIFEVLFVHKYSNKMALNDA 121

Query: 123 TITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGG 182
            +TS+SYLLFT  TIYAQ++SQDLAEPEIDLKA GL MYGIGITGNFYHH+LLSRLR  G
Sbjct: 122 IMTSISYLLFTTGTIYAQMLSQDLAEPEIDLKAVGLVMYGIGITGNFYHHILLSRLRREG 181

Query: 183 DREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKW 242
           DREYRIPRGGLF+VIVCPHYLFEIVEFLGI  VSQTLFA VFV+GS LFLIGRSY TR W
Sbjct: 182 DREYRIPRGGLFKVIVCPHYLFEIVEFLGIVLVSQTLFAMVFVLGSTLFLIGRSYTTRNW 241

Query: 243 YLSKFEDFPSHVKAILPFVF 262
           YLSKFEDFP HVKAILPFVF
Sbjct: 242 YLSKFEDFPRHVKAILPFVF 261

[Close]

BLAST of PI0023313 vs. NCBI nr
Match: XP_038892722.1 (steroid 5-alpha-reductase DET2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 396.0 bits (1016), Expect = 2.6e-106
Identity = 213/262 (81.30%), Postives = 224/262 (85.50%), Query Frame = 0


Query: 3   ATAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAMLL 62
           A+ FLFSPFSS FLL+LS LTFL  L L S EFLG HL+YSKFWTPNSK HLPSRSAMLL
Sbjct: 2   ASTFLFSPFSSSFLLALSFLTFLVTLLLASAEFLGRHLRYSKFWTPNSKFHLPSRSAMLL 61

Query: 63  CYLPAFSLSSL-SLFLFPHHFLLSSRIFLIISLLLFHFFKRISE--VLFVHKYSNKMALT 122
           CY PA SLSS+ SLFLFPHHF   SRIFLI SL   HFFKRI E  VLFVHKYSNKMAL 
Sbjct: 62  CYFPALSLSSISSLFLFPHHFPHFSRIFLINSLFFIHFFKRIFEVHVLFVHKYSNKMALN 121

Query: 123 DATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRS 182
           DA +TS+SYLLFT  TIYAQ++SQDLAEPEIDLKA GL MYGIGITGNFYHH+LLSRLR 
Sbjct: 122 DAIMTSISYLLFTTGTIYAQMLSQDLAEPEIDLKAVGLVMYGIGITGNFYHHILLSRLRR 181

Query: 183 GGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATR 242
            GDREYRIPRGGLF+VIVCPHYLFEIVEFLGI  VSQTLFA VFV+GS LFLIGRSY TR
Sbjct: 182 EGDREYRIPRGGLFKVIVCPHYLFEIVEFLGIVLVSQTLFAMVFVLGSTLFLIGRSYTTR 241

Query: 243 KWYLSKFEDFPSHVKAILPFVF 262
            WYLSKFEDFP HVKAILPFVF
Sbjct: 242 NWYLSKFEDFPRHVKAILPFVF 263

[Close]

BLAST of PI0023313 vs. NCBI nr
Match: XP_022985155.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima])

HSP 1 Score: 376.3 bits (965), Expect = 2.1e-100
Identity = 198/260 (76.15%), Postives = 227/260 (87.31%), Query Frame = 0


Query: 6   FLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTP---NSKSHLPSRSAMLL 65
           FLFSPFSS F+L+LSSL  LS L L + EFLG HLQYSKFW+P   ++K HLPSRSAMLL
Sbjct: 4   FLFSPFSSPFILALSSLIMLSTLVLATSEFLGRHLQYSKFWSPKNSSNKIHLPSRSAMLL 63

Query: 66  CYLPAFSLSSLSLF-LFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDA 125
           CY+PAF+++S+SLF L P +   S+RIFLI SLLL HFFKRI EV+FVHKYSNKM L+DA
Sbjct: 64  CYVPAFAVASISLFLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVVFVHKYSNKMGLSDA 123

Query: 126 TITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLSRLRSGG 185
            I S++Y+L TG+TIY+Q+V+QDLAEP+IDL+AAGLAMY IGITGN YHHVLLS+LR  G
Sbjct: 124 IIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLSKLRKEG 183

Query: 186 DREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKW 245
           DREYRIPRGG+FEVIVCPHYLFE+VEFLGIAFVSQTLFA VF +GSALFLIGRSYATR W
Sbjct: 184 DREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRSYATRNW 243

Query: 246 YLSKFEDFPSHVKAILPFVF 262
           Y+SKFEDFP HVKAILPFVF
Sbjct: 244 YVSKFEDFPRHVKAILPFVF 263

[Close]

BLAST of PI0023313 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 225.3 bits (573), Expect = 5.6e-59
Identity = 124/267 (46.44%), Postives = 173/267 (64.79%), Query Frame = 0


Query: 4   TAFLFSPFSSLFLLSLSSLTFLSILFLVSGEFLGHHLQYSKFWTPNSK--------SHLP 63
           T+F++ P  S+ L  +S +   ++  +   E  G+HL+YSKF   +S           + 
Sbjct: 5   TSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERFGSIS 64

Query: 64  SRSAMLLCYLPAFSLSSLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNK 123
           SR+ MLL Y PAF  ++ S F+ P   L   R  L+ S L  HFFKR+ EVLF+HKYS  
Sbjct: 65  SRNGMLLLYTPAFLAAASSFFVVPSDDL---RFLLLKSALALHFFKRVFEVLFIHKYSGG 124

Query: 124 MALTDATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLS 183
           MA+  A   S SY   T   +Y+Q ++  L EP  D+K AG+ M+ +GI GN YHHVLL+
Sbjct: 125 MAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLA 184

Query: 184 RLR-SGGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGR 243
           +LR   G +EY+IP+GGLF++I+CPHYLFEI+ F     +SQT+++  F +G+ L+LIGR
Sbjct: 185 KLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGR 244

Query: 244 SYATRKWYLSKFEDFPSHVKAILPFVF 262
           SYATR WYLSKF+DFP H+KA++PFVF
Sbjct: 245 SYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of PI0023313 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 75.9 bits (185), Expect = 5.5e-14
Identity = 54/157 (34.39%), Postives = 80/157 (50.96%), Query Frame = 0


Query: 97  FHFFKRISEVLFVHKYSNKMALTDATITSLSYLLFTGTTIYAQIVSQDLAEPEIDLKAAG 156
           FH+FKRI E  FVH++S+  +       + +Y    G  I A  V+  L  P  DL+   
Sbjct: 140 FHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMK- 199

Query: 157 LAMYGIGI---TGNFYHHVLLSRLRS-GGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIA 216
              +G G+     NFY H+LL  LR   G   Y+IPRG LF ++ C +Y  EI ++LG  
Sbjct: 200 -IGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFN 259

Query: 217 FVSQTLFATVFVVGSALFL----IGRSYATRKWYLSK 246
             +QT+   VF+  +AL +    +G+    RK +  K
Sbjct: 260 IATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGK 293

[Close]

BLAST of PI0023313 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 7.4e-11
Identity = 57/200 (28.50%), Postives = 97/200 (48.50%), Query Frame = 0


Query: 72  SLSLFLFPHHFLLSSRIFLIISLLLFHFFKRISEVLFVHKYSNKMALTDATITSLSYLLF 131
           +L LF F  H L    + L    L+ +F + I   L + + S         IT ++ L F
Sbjct: 64  TLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPIT-IAALAF 123

Query: 132 TGTTIYAQIVSQDLAEPEIDLK---------AAGLAMYGIGITGNFYHHVLLSRLRSGGD 191
           T   +   I ++ ++  + D +           G+ ++  G+  N      L RL+    
Sbjct: 124 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 183

Query: 192 REYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATVFVVGSALFLIGRSYATRKWY 251
             Y IPRGG FE++ CP+Y  E +E+LG A ++ +     F + +   L  R+ A+ KWY
Sbjct: 184 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 243

Query: 252 LSKF-EDFPSHVKAILPFVF 262
           ++KF E++P   KA++PFV+
Sbjct: 244 IAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of PI0023313 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.3e-09
Identity = 73/271 (26.94%), Postives = 114/271 (42.07%), Query Frame = 0


Query: 29  FLVSGEFLGHHLQYSKFWTPNSKSHLPSRSAMLLCYLPAFS-LSSLSLFLFPHHFLLSSR 88
           F V  +F GH       WT    + L + + M  C +   S + S  +    H F +   
Sbjct: 58  FTVPQKFFGHFYVVGVVWT----TLLLAATWMYACKMAGGSHVFSFHMTHVEHRFKVGRA 117

Query: 89  IFLIISLLLFHFFKRISEVLFVHKYSNKMAL-TDATITSLSYLL---------------- 148
           +FL++ L+  H  +R+ E  +V KYS    +   A + +L Y +                
Sbjct: 118 VFLLL-LMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPLSLCSNIAPEVAR 177

Query: 149 FTGTTIYAQIVSQDLAEPEIDL--------KAAGLAMYG--IGITGNFYH---HVLLSRL 208
           F G+ +   I S      + +L        K   L   G  I + G  +    H +L  L
Sbjct: 178 FVGSQVAEFIASGKSHSHDFNLLLSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGSL 237

Query: 209 RS--GGDREYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATV-----FVVGSALF 262
           R      +EY IP G  FE++ CPH+L EIV +LG+   S     ++     FV  +  +
Sbjct: 238 REYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLTY 297

[Close]

BLAST of PI0023313 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-08
Identity = 34/98 (34.69%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 171 HVLLSRLRSGGD--REYRIPRGGLFEVIVCPHYLFEIVEFLGIAFVSQTLFATV-----F 230
           H +L  LR      +EY IP G  F ++  PH+L EIV + G+   S     T+     F
Sbjct: 249 HAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF 308

Query: 231 VVGSALFLIGRSYATRKWYLSKFEDFPSHVKAILPFVF 262
           V  +  +  G    T +WYL KFE++P++  AI P+V+
Sbjct: 309 VAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

The following BLAST results are available for this feature:

BLAST of PI0023313 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	3.7e-15	27.63	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
Q2QDF6	8.2e-15	30.04	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
P24008	2.4e-14	38.10	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
P18405	3.1e-14	28.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q55C17	3.5e-13	29.15	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]

BLAST of PI0023313 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BFS4	1.3e-127	94.25	steroid 5-alpha-reductase DET2-like OS=Cucumis melo OX=3656 GN=LOC103489358 PE=3...	[more]
A0A0A0KUP2	3.8e-124	91.95	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]
A0A6J1JAL0	1.0e-100	76.15	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GYE2	4.0e-97	73.95	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1DCA9	7.3e-91	70.80	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of PI0023313 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446721.1	2.6e-127	94.25	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo]	[more]
XP_004135372.1	7.9e-124	91.95	steroid 5-alpha-reductase DET2 [Cucumis sativus]	[more]
XP_038892723.1	8.0e-108	81.92	steroid 5-alpha-reductase DET2-like isoform X2 [Benincasa hispida]	[more]
XP_038892722.1	2.6e-106	81.30	steroid 5-alpha-reductase DET2-like isoform X1 [Benincasa hispida]	[more]
XP_022985155.1	2.1e-100	76.15	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima]	[more]

BLAST of PI0023313 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	5.6e-59	46.44	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	5.5e-14	34.39	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	7.4e-11	28.50	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	5.3e-09	26.94	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.5e-08	34.69	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 151..261 e-value: 1.7E-27 score: 96.2
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 156..225 score: 24.192867
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 6..261
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 6..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0023313.1	PI0023313.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors