PI0023291 (gene) Melon (PI 482460) v1

Overview
NamePI0023291
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionUbiquitin-conjugating enzyme E2 32
Locationchr06: 5395767 .. 5398475 (+)
RNA-Seq ExpressionPI0023291
SyntenyPI0023291
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCCAATACCCAATTTCATTGTTTCGCTCCCCTTCTTCCTCCGTGCCAATAAATTGCGACTGCCATCAATCCGCACGGTTTGGCTTGGATTATCGATTCAAATCCTCTCGTTTGGTTCCGGAGATTGCTTCTTTTCCGATCAAGTAATTTTGTAGTCTTGTGATCGGTGGTGGTTGCAGTCGGATCGGATCTGCGATTGAATTGAGAAGCTATGGCGGAGGATAAGTACAATTTGAAGAATCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTCATGAGTCTTCCTCTTGAGGTTTGTTGCTTCTGATTTTCTCTGAATTTCTATTTCTGTTTTAAAATTTAACCTAATGGGTGTTGTTCTTGTTTGGTCAGTTTTACATTCTAATGGTTTATGTTTCTCAGTTGAATGAATTGTTTCATTGTGATTATCTCCGATTATTGAATTGCTACGATGTATGCGGAAGTGTGGACGGAATCAATTATCAATTTGGTTTTCTTTCATTTTTTCTTACCTACTCTACTGGGGTCTGCGACTAGTCGCATATGGATGTCCCGTTAGGGCTCAAAAAGGAAGAAAGTGAATATATTAATCGGTTTTTTGGGTTAAATTTTATTTACTATTCATTCGAGTTTACAATCTTCGAAAATTATCGACGGCTGAAAGTTTGGGGCAGTCCTTTGTTTTGAATAAACCCTTGCTCTCTAGTTAGGCTAATAATCAATTTGTTTAAATTGATTGCTAAATCTACGAACACATGCGCATTCGGTCATTTTGCATAAATCTTCATTCATCTCATGTGTCTTGATTCTTATAGTTGTGTCCACTTCATTGCATTTCGGATAAAGAAAAGAAAAGAAAGAAAATATCGGCTATGGGAACTAGGAAGCTTTGCTTGATTGATACACTAATCTTATAATTATGTGCGTAATTTTGTATTTCAATTGTTATAGAATTTGGGGTTTTTCCAAACCTCCCTGTTGAATTTGAGGCTAAATGTTGGTTTTCCAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGCTGTTGACAGTAAGATAGTAATCTCTAATCATGCTTGCTTCCCTTTCTGTCGGCTTATATAGAGCATGTTTGAAATCACTTTTCATATCCAATACACTTTTAAACACAAATTTTCGTACAATCAAAATTCATTTTGAATGATTAGAAGTAATCTCTCAGAGTGAATAGAAGACTAGGATTATTCTAACAATTTCATAATCACCCTCAAACAAGCCTATAGTTCTTCTGTTGCGTATTTTGATAAGTGATGTTATACTTAATGGAAGAGCTTATCAATGTCAATTCCTTTCTTTCAGCCGAATGGTCGATTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGGTGCGTTCTTTTTATTCTTTATTAAACTTCTCTTGTCATCTTATGTTATGTTAAACTCAAAGGATTTTCATTGTCTGGTTTTTACATTTACATTTAGTTGCTTCAAGTTTCTTTTGTCCTGGCTTCCCAATTTTTGTTGATTACTGTTTGTAGTGCGCACTGCATTGGTGGCATTAATTGCTTTCATGCCCACAAACCCAAATGGAGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCCCCGAAATTTGGTACTCCTGAACGTCAAAAACTAATTGACGAGGTACTCCTGAGTTCTGTTGAAATTTTGTAAGCTATCGAGTAGTAATGTATTGAATCTCCATCATGTGATATATCATTCTTAACTAATCAGCTCCTTTACATCTCAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTTCCTCAATCGGGTTCTTCAGATGTCTCTGGCGAGAAACTCACCAACGAGGAGGCTGACGTCCAGGTAACCTCTGAACCTGCTGAACTTGCAGCTGCTGGGGAAGAGCTGCAGAATCCACCTACAGTCAACAGGATCGTTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCAAGAGTGGCGCGATCTAGTCGCCCCACCACTCAGGCTGAGCAGCGGAGGCCTGAGACGGTTGTTCATAAACCAGCCGATGACAGGTTGTTCACATGGGCTGCGATCGGGCTCACTCTTGCAATCGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAGAAGATTGGAGATGATATTTGTTGGTTGATAGCTTTGCTATGAGACACAAGGGTCTTATGTGTATTGATAGTTTTAGCGTAAATTCTAATGTTTCTTATCATTCTGATTCTACAAGGAAAAAAGCAATCTTATGTTTTTATGTTGTTGCCTTCTCCATTATTTGTAGTTAAATAATAACATATGCAAAATTGCTTGGCTTAAAGAGTGTGCGTACAAACCTTTTTCATTTCCTGGTTGTCTGTTTTATGTACAAGGTATTATTAGCCATACTTGAAATGGGTTATTCTTTTGGGCAAGTCTAGATTGTAATTGATCATTGACACTATTCACCTAAAAATTTG

mRNA sequence

CGCCAATACCCAATTTCATTGTTTCGCTCCCCTTCTTCCTCCGTGCCAATAAATTGCGACTGCCATCAATCCGCACGGTTTGGCTTGGATTATCGATTCAAATCCTCTCGTTTGGTTCCGGAGATTGCTTCTTTTCCGATCAAGTAATTTTGTAGTCTTGTGATCGGTGGTGGTTGCAGTCGGATCGGATCTGCGATTGAATTGAGAAGCTATGGCGGAGGATAAGTACAATTTGAAGAATCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTCATGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGCTGTTGACACCGAATGGTCGATTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGTGCGCACTGCATTGGTGGCATTAATTGCTTTCATGCCCACAAACCCAAATGGAGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCCCCGAAATTTGGTACTCCTGAACGTCAAAAACTAATTGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTTCCTCAATCGGGTTCTTCAGATGTCTCTGGCGAGAAACTCACCAACGAGGAGGCTGACGTCCAGGTAACCTCTGAACCTGCTGAACTTGCAGCTGCTGGGGAAGAGCTGCAGAATCCACCTACAGTCAACAGGATCGTTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCAAGAGTGGCGCGATCTAGTCGCCCCACCACTCAGGCTGAGCAGCGGAGGCCTGAGACGGTTGTTCATAAACCAGCCGATGACAGGTTGTTCACATGGGCTGCGATCGGGCTCACTCTTGCAATCGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAGAAGATTGGAGATGATATTTGTTGGTTGATAGCTTTGCTATGAGACACAAGGGTCTTATGTGTATTGATAGTTTTAGCGTAAATTCTAATGTTTCTTATCATTCTGATTCTACAAGGAAAAAAGCAATCTTATGTTTTTATGTTGTTGCCTTCTCCATTATTTGTAGTTAAATAATAACATATGCAAAATTGCTTGGCTTAAAGAGTGTGCGTACAAACCTTTTTCATTTCCTGGTTGTCTGTTTTATGTACAAGGTATTATTAGCCATACTTGAAATGGGTTATTCTTTTGGGCAAGTCTAGATTGTAATTGATCATTGACACTATTCACCTAAAAATTTG

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATTTGAAGAATCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTCATGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGCTGTTGACACCGAATGGTCGATTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGTGCGCACTGCATTGGTGGCATTAATTGCTTTCATGCCCACAAACCCAAATGGAGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCCCCGAAATTTGGTACTCCTGAACGTCAAAAACTAATTGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTTCCTCAATCGGGTTCTTCAGATGTCTCTGGCGAGAAACTCACCAACGAGGAGGCTGACGTCCAGGTAACCTCTGAACCTGCTGAACTTGCAGCTGCTGGGGAAGAGCTGCAGAATCCACCTACAGTCAACAGGATCGTTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCAAGAGTGGCGCGATCTAGTCGCCCCACCACTCAGGCTGAGCAGCGGAGGCCTGAGACGGTTGTTCATAAACCAGCCGATGACAGGTTGTTCACATGGGCTGCGATCGGGCTCACTCTTGCAATCGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAG

Protein sequence

MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSSRPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Homology
BLAST of PI0023291 vs. ExPASy Swiss-Prot
Match: Q9LSP7 (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.9e-108
Identity = 204/310 (65.81%), Postives = 236/310 (76.13%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+PERQK+IDEIH+Y+LSKA  VP+  
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
             + S       EA  QV  EP E     EE ++  T + IV++ + E   E    A S 
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQEAITVVEE-RSIATTDTIVDDQIIEETAEAVNTAASV 240

Query: 241 RP--------------TTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKA 297
            P              +   EQR       KP DDRLFTWAA+GLT+AI+VLLLKKF+K+
Sbjct: 241 VPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKS 300

BLAST of PI0023291 vs. ExPASy Swiss-Prot
Match: Q9Y385 (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 211.1 bits (536), Expect = 1.7e-53
Identity = 94/146 (64.38%), Postives = 118/146 (80.82%), Query Frame = 0

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE 149
           T   GA+GSLDY  EERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

BLAST of PI0023291 vs. ExPASy Swiss-Prot
Match: Q9JJZ4 (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 211.1 bits (536), Expect = 1.7e-53
Identity = 94/146 (64.38%), Postives = 118/146 (80.82%), Query Frame = 0

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE 149
           T   GA+GSLDY  EERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

BLAST of PI0023291 vs. ExPASy Swiss-Prot
Match: Q6FQK7 (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.6e-33
Identity = 61/135 (45.19%), Postives = 91/135 (67.41%), Query Frame = 0

Query: 12  AVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K M  NP    ++ P EENI EW + I GP D+ ++GG YHG +  P++YP
Sbjct: 6   AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           +KPP+  ++TPNGRF+  T++CLS+S++HP+ W P WSV T L  L++FM T      GS
Sbjct: 66  YKPPAIRMITPNGRFKENTRLCLSMSDYHPDTWNPGWSVATILNGLLSFM-TGDESTTGS 125

Query: 132 LDYKKEERRVLAIKS 147
           +   ++E+++LA KS
Sbjct: 126 ITTTQQEKKILAKKS 139

BLAST of PI0023291 vs. ExPASy Swiss-Prot
Match: Q74Z34 (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-31
Identity = 59/137 (43.07%), Postives = 88/137 (64.23%), Query Frame = 0

Query: 12  AVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K M  NP    ++ P E+NI  W + I GP ++ +E G YHG +  P +YP
Sbjct: 6   AYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           F PP+  +LTPNGRF   T++CLS+S++HP+ W PSWSV T L  L++FM T+   ++G+
Sbjct: 66  FNPPAIRMLTPNGRFRENTRLCLSMSDYHPDTWNPSWSVATILTGLLSFMTTD-ESSIGT 125

Query: 132 LDYKKEERRVLAIKSRE 149
           ++     +R  A KS+E
Sbjct: 126 MESTDSTKRKYAAKSKE 141

BLAST of PI0023291 vs. ExPASy TrEMBL
Match: A0A5A7U7K5 (Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001470 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.3e-160
Identity = 288/297 (96.97%), Postives = 292/297 (98.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
           SSDVS EK TNEE+DVQVTSEPAELAAAGEELQNPPT+NRIVEEAVPEVQPEVARVA SS
Sbjct: 181 SSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQAEQ+RPET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of PI0023291 vs. ExPASy TrEMBL
Match: A0A1S3AXJ7 (ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 2.9e-160
Identity = 287/297 (96.63%), Postives = 292/297 (98.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
           SSDVS +K TNEE+DVQVTSEPAELAAAGEELQNPPT+NRIVEEAVPEVQPEVARVA SS
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQAEQ+RPET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of PI0023291 vs. ExPASy TrEMBL
Match: A0A0A0L954 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.3e-159
Identity = 287/297 (96.63%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
           S+DVSGEK TNEEADVQVTSE AELAAA EELQN PT+NRIVEEAVPEVQPEVARVA SS
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQ EQ RPET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of PI0023291 vs. ExPASy TrEMBL
Match: A0A6J1C9I9 (ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC111009459 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.2e-139
Identity = 263/307 (85.67%), Postives = 270/307 (87.95%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAE+KYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPSDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFG+PERQKLIDEIHEY+LSKAPP+PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGSPERQKLIDEIHEYILSKAPPIPQPS 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPE-------VQPEV 240
            SD          ADVQVTSEPAELAAAGEELQNPP  +RIVEEAVPE        QPEV
Sbjct: 181 PSD----------ADVQVTSEPAELAAAGEELQNPPAGDRIVEEAVPEEVTTNANPQPEV 240

Query: 241 ARVAR---SSRPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHG 298
            RVAR   SSR  T   Q R ET V KPADDRLFTWAA+GLTLAIVVLLLKKFMKASAHG
Sbjct: 241 VRVAREVPSSRAATAQVQMRAETRVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKASAHG 297

BLAST of PI0023291 vs. ExPASy TrEMBL
Match: A0A6J1FCW1 (ubiquitin-conjugating enzyme E2 32-like OS=Cucurbita moschata OX=3662 GN=LOC111444487 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.9e-138
Identity = 257/304 (84.54%), Postives = 269/304 (88.49%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREA PKFGTPERQKLIDEIHEY+LSKAPP PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPE-------VQPEV 240
           S D S EK  N+EADV+VTSEP E AA GEE QNPP  +RIVEEA+PE       +Q   
Sbjct: 181 SPDDSEEKPANKEADVEVTSEPTEPAATGEEQQNPPGSDRIVEEAIPEETSINTNLQQAA 240

Query: 241 ARVARSSRPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVF 298
           AR A S RP   A Q R ET V+KPADDRLFTWAA+GLTLAIVVLLLKKF+KASAHGSVF
Sbjct: 241 AREAPSDRP---AVQLRTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASAHGSVF 300

BLAST of PI0023291 vs. NCBI nr
Match: KAA0049541.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa])

HSP 1 Score: 575.5 bits (1482), Expect = 2.7e-160
Identity = 288/297 (96.97%), Postives = 292/297 (98.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
           SSDVS EK TNEE+DVQVTSEPAELAAAGEELQNPPT+NRIVEEAVPEVQPEVARVA SS
Sbjct: 181 SSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQAEQ+RPET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of PI0023291 vs. NCBI nr
Match: XP_008438945.1 (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo])

HSP 1 Score: 574.3 bits (1479), Expect = 6.0e-160
Identity = 287/297 (96.63%), Postives = 292/297 (98.32%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
           SSDVS +K TNEE+DVQVTSEPAELAAAGEELQNPPT+NRIVEEAVPEVQPEVARVA SS
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQAEQ+RPET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of PI0023291 vs. NCBI nr
Match: XP_004134243.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical protein Csa_009813 [Cucumis sativus])

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-158
Identity = 287/297 (96.63%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
           S+DVSGEK TNEEADVQVTSE AELAAA EELQN PT+NRIVEEAVPEVQPEVARVA SS
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQ EQ RPET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of PI0023291 vs. NCBI nr
Match: XP_038895230.1 (ubiquitin-conjugating enzyme E2 32 [Benincasa hispida])

HSP 1 Score: 558.1 bits (1437), Expect = 4.4e-155
Identity = 285/301 (94.68%), Postives = 289/301 (96.01%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPE-VARVAR- 240
           SSDVSGEK TNEEADVQVTSEPAELAA+GEELQNPP  +R+VEE VPEV+PE VARVAR 
Sbjct: 181 SSDVSGEKPTNEEADVQVTSEPAELAASGEELQNPPANDRVVEEVVPEVRPEVVARVARE 240

Query: 241 --SSRPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 298
             SSRP  QAEQRR ET VHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE
Sbjct: 241 VPSSRPPAQAEQRRLETGVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 300

BLAST of PI0023291 vs. NCBI nr
Match: XP_022138234.1 (ubiquitin-conjugating enzyme E2 32 [Momordica charantia])

HSP 1 Score: 505.0 bits (1299), Expect = 4.5e-139
Identity = 263/307 (85.67%), Postives = 270/307 (87.95%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAE+KYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPSDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFG+PERQKLIDEIHEY+LSKAPP+PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGSPERQKLIDEIHEYILSKAPPIPQPS 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPE-------VQPEV 240
            SD          ADVQVTSEPAELAAAGEELQNPP  +RIVEEAVPE        QPEV
Sbjct: 181 PSD----------ADVQVTSEPAELAAAGEELQNPPAGDRIVEEAVPEEVTTNANPQPEV 240

Query: 241 ARVAR---SSRPTTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHG 298
            RVAR   SSR  T   Q R ET V KPADDRLFTWAA+GLTLAIVVLLLKKFMKASAHG
Sbjct: 241 VRVAREVPSSRAATAQVQMRAETRVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKASAHG 297

BLAST of PI0023291 vs. TAIR 10
Match: AT3G17000.1 (ubiquitin-conjugating enzyme 32 )

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-109
Identity = 204/310 (65.81%), Postives = 236/310 (76.13%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+PERQK+IDEIH+Y+LSKA  VP+  
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SSDVSGEKLTNEEADVQVTSEPAELAAAGEELQNPPTVNRIVEEAVPEVQPEVARVARSS 240
             + S       EA  QV  EP E     EE ++  T + IV++ + E   E    A S 
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQEAITVVEE-RSIATTDTIVDDQIIEETAEAVNTAASV 240

Query: 241 RP--------------TTQAEQRRPETVVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKA 297
            P              +   EQR       KP DDRLFTWAA+GLT+AI+VLLLKKF+K+
Sbjct: 241 VPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKS 300

BLAST of PI0023291 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 122.9 bits (307), Expect = 4.4e-28
Identity = 68/188 (36.17%), Postives = 103/188 (54.79%), Query Frame = 0

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPVPQSG 189
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  PQ  
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

BLAST of PI0023291 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 122.9 bits (307), Expect = 4.4e-28
Identity = 68/188 (36.17%), Postives = 103/188 (54.79%), Query Frame = 0

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPVPQSG 189
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  PQ  
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

BLAST of PI0023291 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 122.5 bits (306), Expect = 5.8e-28
Identity = 65/190 (34.21%), Postives = 100/190 (52.63%), Query Frame = 0

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKS----------REAPPKFGTPERQKLIDE----IHEYMLSKAPPVPQ 189
           +    E++ LA  S          R+  P++     Q+ + E      +   S+    PQ
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQ 186

BLAST of PI0023291 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 122.5 bits (306), Expect = 5.8e-28
Identity = 65/190 (34.21%), Postives = 100/190 (52.63%), Query Frame = 0

Query: 13  VKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P    ++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKS----------REAPPKFGTPERQKLIDE----IHEYMLSKAPPVPQ 189
           +    E++ LA  S          R+  P++     Q+ + E      +   S+    PQ
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQ 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LSP71.9e-10865.81Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2... [more]
Q9Y3851.7e-5364.38Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2[more]
Q9JJZ41.7e-5364.38Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2[more]
Q6FQK72.6e-3345.19Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 13... [more]
Q74Z342.5e-3143.07Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 10... [more]
Match NameE-valueIdentityDescription
A0A5A7U7K51.3e-16096.97Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXJ72.9e-16096.63ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 ... [more]
A0A0A0L9549.3e-15996.63UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE... [more]
A0A6J1C9I92.2e-13985.67ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC11100945... [more]
A0A6J1FCW16.9e-13884.54ubiquitin-conjugating enzyme E2 32-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
KAA0049541.12.7e-16096.97ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiqui... [more]
XP_008438945.16.0e-16096.63PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo][more]
XP_004134243.11.9e-15896.63ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical pr... [more]
XP_038895230.14.4e-15594.68ubiquitin-conjugating enzyme E2 32 [Benincasa hispida][more]
XP_022138234.14.5e-13985.67ubiquitin-conjugating enzyme E2 32 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G17000.11.3e-10965.81ubiquitin-conjugating enzyme 32 [more]
AT1G17280.14.4e-2836.17ubiquitin-conjugating enzyme 34 [more]
AT1G17280.24.4e-2836.17ubiquitin-conjugating enzyme 34 [more]
AT5G50430.15.8e-2834.21ubiquitin-conjugating enzyme 33 [more]
AT5G50430.25.8e-2834.21ubiquitin-conjugating enzyme 33 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..174
e-value: 6.9E-28
score: 108.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..254
NoneNo IPR availablePANTHERPTHR24068:SF303BNAC01G21910D PROTEINcoord: 7..297
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 7..297
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..177
e-value: 7.1E-47
score: 161.2
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 12..134
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 15..126
e-value: 5.5E-20
score: 71.5
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 11..166
score: 37.480679
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 13..152
e-value: 5.32113E-45
score: 147.734

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0023291.1PI0023291.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity