PI0023023 (gene) Melon (PI 482460) v1

Overview


Name	PI0023023
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	tRNA (Guanine(9)-N1)-methyltransferase
Location	chr12: 15690070 .. 15696312 (+)
RNA-Seq Expression	PI0023023
Synteny	PI0023023

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAATCGTAAAACAATTAGATAGGAATGGATCCCCCGACAAGGTTAGTTCACCGTCATCATTTGGGCCAGGGCAATTTACAGTTACCCTAGTGTACTCTTCATTTGGTAGCCAACAATGGAGGCTGTAGAAGAGGACCACAGCTCTAAAATGGATCCTCCTTGTTTCCCGTCCCCCTGCAATCCTCCTGAACCTCCCCTCTCCAAGAATGCCAGGAAGAAGCTTCTGAAGCAACAACGATACGAAGCGAAGAAAGCGGACAAGAAACTTCAGGCTAAAGAGCAGAAGAAGAAAGACATCGAGCGAAAACGGAAGGAATGGGAAGAGAAACTGGCCAGTGTTACGGAGGAGGAGAGAGCCAAACTGATCGAGTCTCGGAGAAGTCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGACAGGGAGAAGAAAATTGAGAGACTCAACACCGCCAAAGATTGTGGCCAAAATATTGTCATCGACCTTGAATTTTCTCACCTCATGACCCCTTCGGAGATTCACAGTCTTGTCCAGCAGGTCTTTTTTTCTTGTTACTCACACTTCACTTGGTTCATTAACTATTTGTGGTGCGAATCGAATACTTTTTAATACTATTTTTTTTTTTTTTTTTTTTTTACTTTTAAAATGCTGTCTATCTATTTAAGAGGTTTGTTAAATGTTTCTAGTAGATGGAAATGGAATGAACGGCTTCTTCTATGGTGCCCTTTAAGAAGTGAAATTGGTTGTTATTCTTGGAAGACAGTTTCTTAGCCAAATAGTTTGAAAAAGGCAAGCCTTGGCAGACTTAAATCTAGCGTTAGTAGTTGATAAAGTAACAAGTGGATGTTTCTTGGAAAACCACCAAATGCTGCCTGAGATCAACACAAACGCATGACTTGAACTGCTCCTCCCATCATTTCAATCTCCCAGCGTAATTACTACCGAAATTTGATTTTCTTATTTTTATTTCCCACTTTTGTAGAACAACCCATCATTTTTGGTCTCTTTTTGCAAGTAATAAAAGATCCTTTCAGTATCCCAACAAGACGCAACTGCGTTGGATTCTCCCAAAATCTTGTTTCTTGCAGGTGAATGCATCAAACTACCCTCAATTTGATTATAATCTATGTCGTTCACCTTCTTTCTTCTTTCCTTCATGGTCTTTATTTAGTTCCAAGCCAGACTCAGTTGGAGTACATATAGAATTGCAATCCTTCATTTGAATACTATTAAAATTTCTTGCTACAAAGTTTCGAAACTCTAAGGAGCTCCTTCTACACAACTATTTTAAAAAACTTCTTTCTCCTTTTCCCTTTCCTCTATGTATGACGAAATACCATAACAACCTCCTTAACTAATTACTAATATACTTTTAGTATCCAAATAAAAACCTAATGGCATTCCTATCACCAAGCTCTTGGAAGATGTTTTAGTTTATTACTTTTCAGTAATTTGTATGCACGATGCTAATCTCCAGTCTTATAACATAGTGGTTTCAGTGGGAGAATTGGTTAAATCTTTTGTCTTTGATATGCAATCAACCAAATGAAGCTTCCTTATAATTTCCTTAGTATGTTGGATAAATTCCCCAAGCATTTTGACAATGAATGAAAGGTGTCTATAGATCACCTATCAAGTATTCAAGTGGGATATTGCATACTTTGATCTGAAAAATACCTGCTTTACCTACGGCTCTAGACACCAGTTGTAGTCTGCAAGATACCCTACTGACTTTCTAATATGGTGCATTTCTAGGTGCTAAAAAATGTTAAGGGAGTGCACTTATCAATTAAATGAAATGGAAACAGACCAATAAAATGATCTACATTTGCAGGATAGTAGGATCTTTTTTTTTTTATATCCGTGAGTGTCCGGACCAACTTACGTACACCTCAACTAATCTCACATAACAACGAACCTGACCTACAACATTTGGACGTCAAGGAAACTCGTAGGAAATTAATTGCTAGGGAGATGACCACCATCGATTGAACCATGACCTCTTAGCTAGTTATTGAGACTATGTTTTCCTTTTTACCACTAGGCTAATCCATGATGGTTCGCAATTATTATTCTTTTGATCTATCTCTCACAGTGAGGAGTAGCTTGAGCTTCTACCGTGGGATTCTTATCTTCCGTTTCCAACATTAGAAGTTTGTGTGTATGTGTAAGAAACAATCATTGAGATGTAATTACAAAAAAACCAAACTACGTGCCAAAGGAATAACAAAAAACACTTCAATTGGGGCATAAGAGAAGTGAGGTTATAGGAGGTAAAGAGAAGGTTAAGTTTTACACTAAAAGAGAGCATTAAAGCAATATGTATGATTTACATCAATATAAATTTCTTGATCTTGTTTCGCTGTTTTATAGGGAAAAAAACCCCTTAAAATGAAGTTTGAATCTTCCCAAAAGAGTTTCCTATTTTTTATTTTTTCTTCAAATAATATTTATTTATTCATTTTAATGGATTTTATGAGGGATCATTAATGTATTTGTTCATATTCATCCTTGGTGATTTTTCAATATAGTCGTCCACTCTTGCCCCTAGACGCAGAACTCGCAGACAAACAATTTTCCATAGTAAAGCCACCAACAGATTTGAAAATTTGATCTCTGAGGGAGTGTTACTTATCCTTGATGTTGAATTACGTATCCCCTGGTCTATTAATGTTACATACTTCCAAGATTGACGTGAGAGATTTCAATATCTACTCCATCTCTATTGGTTCTGTGATATTCCTTCACTTTCTCTAATTGAAACAAAACCAACTAATCTGGTTTGTAGACTTGCCGTCTGCACCATTGTCCTTGGGTAACCTTTTATGATGTCATGGAAGGGTTCCCAACCTTCTCTCCTCCTTCAGGAATGATGGTTGCATTTTTTCATTGCCATTTTTAATTTTTATTTATTTATTTTATTGTTTTAAATTCCAGCAGAAGCATTCTTCAGAGGTAGTCTCAACTCAACGAATGACCATCTCTTGGAAGTCTTAGAATAATTTGGTATTTAGGATATAACTGTAGCCTTGGAATTATCACTCTACGATTATATGGATACGTGGTTGAGAGAGAATGAAAAGAACTATTACTTCATTCAGACAGCCTTGGATAATGGATACCTACTAGCCACCTTGATCTTTAACCAATCAAATGCTTGACTAATCCCACCCTGTCAAAACACTCAAGAACCATTGGAAGATGGAGACAATAGAATAAAGAAAAAGAGGAGAGAGCATCATAGGAGTCACCCTTTTCAAAATTTAAACTTTTTACGTTTTTAATAAAAGCATAAATTTGTAGGATTTAACTCTGAATCTTACGATCAAAAGATTTCTAATGGGAGAATATAAGTTTATATTGCACCTTAATGCGTGGTACTTCTACAAAATGTATGATGGGCAGTATTTAATTGGAATAGACTAAGATGACTAAGATGGAATATTTTTTGGAGAAATAAAAATGCATAGCACATTTTACTGAGTACTTTTCCTACATTAGTTAGTTATCAAGCCAAGGCATTTTAAAATACGTCATCATCCCTATGTCATAGCATTTCATAGCTGATTTATTCTTGTCTTCACATTTAGTTATGGTCAGGATGCTGCTTTTTGTTGTGATGTTGAAATGGATCTTTTTCAATATACAAGTGATTCATTTCTGCAGACTCTACTGAAAACTTTGACGTTTTATCCTCATGCAGCATTCTTCTTTATTTTGTATGTTAATTCTCAAATTAATTTAACTAGTAATGATTGAATGATCTCTTGAAAACAGCAAGTTTTCTGAGTATCTTTATTGGGTAAAAACCTGCAGATAATGTATTGTTATGCGGTGAATGGAAGATGTCCTTCTCCTTGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGATAAATGGATCATTGAGAAGGAACGTAGATCATACATAGATGCTTTGCAAGACCAGAAGGAAAATCTGGTATATCTCACAGCAGACTCAGAAACTGTGCTTGATGACCTTGATGTAAAGAAAATTTATATCGTTGGTGGGTTAGTCGATCGTAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTATATCTTGGTTTTTACATGCTAATTATTTTTATCGACATTTCAGGGACTCTAAATTTTGAAATCCTGGACTTACAAAATGGGAGATTAAGAATACAAGTGTGCTCCAATCTTGTAACTACTTATTTTTGTAGAAAAATGAAAGAACGTAGAAGGCATACAAGAAAAGGAACATCTCAAAAAGGAGCCTCAATAAAAGCTACAAAATAGGACTCAAACCAACCAGAATAAAACCCAACTGACAATTAGAAAACCCTTGTGCGCAAAGAGACATTGAATTTAGCAACTTCCCAAATCTCTTCCCGAAACCTTTTAACCCCTTTGGAAATTCTACCATTCCTCTTTAACCAAATACCCCACATAATAGCAAAAAAGGGTTGCTCCTGGGGCAAAAGAACCTCCCCATCATAGAATAACATTCACGGTCAGGTCCTTAATAGATCGCAAATGACTCCAAAAAGTGAATCCAAATTGATTGCGTGAACCAACTATTCCAAAGGATATGGTGGAGATTCTTAAGATGCTTTACACAAATGCACCAATGCAGCACTAACAAAAGGAAAAAGTATCCCAAGTATCAACTTGTCCATGTGATGCTGAGCATTGAATTTGACCTTTGAGATCTCAACCTTCCAAAGAGGAAGAATGAAAGGGGGCTCAAGGAAGGGTGAAGGGACGCATGATAGATGGAAGAGGAAAATATATGGAAAGCTCCTGAAGGGGGCTCACAACTCTGCTGTCTCCTCATTTGATGAAGGATGATGAAAACCAAAATAAATTGAAAGTTCCTGAAAATAAGGGCAAAACTGAAGCTCGCATAATGTAACCTCCATACTGCAAAATTGTAAAGATGTCGAACGAAACATGACCCAAAGAGGCTTATCTCCTATGATCCCACCCTAAAATCCTCTAAAAAAATAAGTATTTGATTCATCTCATGGAAACATTTCATAAATAGGAAAAATATGAAAGCCTGAAAAATTTACATTAGAGACTTTAACCCCCCACTACCCACTGAAACAAGTGTGGAGCATACTTGTTCATAATAACCATATGCCACAAAGCGTTAGGCTCTGTAACGTACCCACTGAGGCCTCCTTTTGATCTCCGAGTTTCCAACAGGTAAAGTTAAAGCCCCACGATTCAACTTATTTTTCTGGATAGAAACCAAGACATTAGACAATTGGTCAAGTACAATGCTCCTTTTCGATAGATTTATGGATATGGTTTTGTCTTCTGCTTTTTATTGGTGCAGGGATAAAGCACCCTTTCAATCATTTCAGTTTATCTTATTAACTCCGATTGAGAATACTTATTGTAGGGTTTTCTCCTTTTCGTTGATCAATGAATGTTTCTTTATCTAAAAACAATGAAACGAATAACAAATAAGAGCTGAGGCATACCTGTAGGCTGTAACAGTGAGTACAAAAGAAATGCTCCTAACCAAATTTTGTAGGGGTGTAAGTGGGTCAATTAGGCTTGTTTTTTGGCCAAAACAAAGTGAGATTAGGCTTTTTGTATATCCGACCTACCAGATTACAAATTGACTTTTTATATTTAGTAATTGTTCTCTTACAATAGTCATTTCCAGGTCCTTACTGTGAACCAAGTCATAGAGATTCTACTCAAGTATTTGGAAACAAGAGATTGGAAGGCTTCTTTCTTTCAGGTTGTTCCTCAAAGAAAAAGACGTGAAGCAGATTCAAACAGTGACCATCCATTAGATGGGGAAGAAAATGGAGATGGAGACAACAAGCACGAGACGAAGAAGACTAAGTGTGATGAAGTATCTTCCAGTGACCATAATTTGTAATGGCTTTGACCAATTGTGAATCAAGTCATAGAGATTCTATTTAAGTATCTTATATTTTTTGGTAGGGTTATTTCTGTGTTAAAAGGATTCTGATTTTTCATTTTTGACTAGTTTATATACTTGCAACGTAGATTGAAAAGGAAGACAAATTTTTGTAAAAGTTTAGTTAAAGAGCCTACTTCTAACAGATGAAAAATAAACAAATAAGTAAATAAATAATGCCTAAATCTGCCCGTTTTTCATGGGGCAATAA

mRNA sequence

AAAAAATCGTAAAACAATTAGATAGGAATGGATCCCCCGACAAGGTTAGTTCACCGTCATCATTTGGGCCAGGGCAATTTACAGTTACCCTAGTGTACTCTTCATTTGGTAGCCAACAATGGAGGCTGTAGAAGAGGACCACAGCTCTAAAATGGATCCTCCTTGTTTCCCGTCCCCCTGCAATCCTCCTGAACCTCCCCTCTCCAAGAATGCCAGGAAGAAGCTTCTGAAGCAACAACGATACGAAGCGAAGAAAGCGGACAAGAAACTTCAGGCTAAAGAGCAGAAGAAGAAAGACATCGAGCGAAAACGGAAGGAATGGGAAGAGAAACTGGCCAGTGTTACGGAGGAGGAGAGAGCCAAACTGATCGAGTCTCGGAGAAGTCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGACAGGGAGAAGAAAATTGAGAGACTCAACACCGCCAAAGATTGTGGCCAAAATATTGTCATCGACCTTGAATTTTCTCACCTCATGACCCCTTCGGAGATTCACAGTCTTGTCCAGCAGATAATGTATTGTTATGCGGTGAATGGAAGATGTCCTTCTCCTTGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGATAAATGGATCATTGAGAAGGAACGTAGATCATACATAGATGCTTTGCAAGACCAGAAGGAAAATCTGGTATATCTCACAGCAGACTCAGAAACTGTGCTTGATGACCTTGATGTAAAGAAAATTTATATCGTTGGTGGGTTAGTCGATCGTAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTCCTTACTGTGAACCAAGTCATAGAGATTCTACTCAAGTATTTGGAAACAAGAGATTGGAAGGCTTCTTTCTTTCAGGTTGTTCCTCAAAGAAAAAGACGTGAAGCAGATTCAAACAGTGACCATCCATTAGATGGGGAAGAAAATGGAGATGGAGACAACAAGCACGAGACGAAGAAGACTAAGTGTGATGAAGTATCTTCCAGTGACCATAATTTGTAATGGCTTTGACCAATTGTGAATCAAGTCATAGAGATTCTATTTAAGTATCTTATATTTTTTGGTAGGGTTATTTCTGTGTTAAAAGGATTCTGATTTTTCATTTTTGACTAGTTTATATACTTGCAACGTAGATTGAAAAGGAAGACAAATTTTTGTAAAAGTTTAGTTAAAGAGCCTACTTCTAACAGATGAAAAATAAACAAATAAGTAAATAAATAATGCCTAAATCTGCCCGTTTTTCATGGGGCAATAA

Coding sequence (CDS)

ATGGAGGCTGTAGAAGAGGACCACAGCTCTAAAATGGATCCTCCTTGTTTCCCGTCCCCCTGCAATCCTCCTGAACCTCCCCTCTCCAAGAATGCCAGGAAGAAGCTTCTGAAGCAACAACGATACGAAGCGAAGAAAGCGGACAAGAAACTTCAGGCTAAAGAGCAGAAGAAGAAAGACATCGAGCGAAAACGGAAGGAATGGGAAGAGAAACTGGCCAGTGTTACGGAGGAGGAGAGAGCCAAACTGATCGAGTCTCGGAGAAGTCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGACAGGGAGAAGAAAATTGAGAGACTCAACACCGCCAAAGATTGTGGCCAAAATATTGTCATCGACCTTGAATTTTCTCACCTCATGACCCCTTCGGAGATTCACAGTCTTGTCCAGCAGATAATGTATTGTTATGCGGTGAATGGAAGATGTCCTTCTCCTTGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGATAAATGGATCATTGAGAAGGAACGTAGATCATACATAGATGCTTTGCAAGACCAGAAGGAAAATCTGGTATATCTCACAGCAGACTCAGAAACTGTGCTTGATGACCTTGATGTAAAGAAAATTTATATCGTTGGTGGGTTAGTCGATCGTAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTCCTTACTGTGAACCAAGTCATAGAGATTCTACTCAAGTATTTGGAAACAAGAGATTGGAAGGCTTCTTTCTTTCAGGTTGTTCCTCAAAGAAAAAGACGTGAAGCAGATTCAAACAGTGACCATCCATTAGATGGGGAAGAAAATGGAGATGGAGACAACAAGCACGAGACGAAGAAGACTAAGTGTGATGAAGTATCTTCCAGTGACCATAATTTGTAA

Protein sequence

MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWIIEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNSDHPLDGEENGDGDNKHETKKTKCDEVSSSDHNL

Homology

[Close]

BLAST of PI0023023 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.5e-37
Identity = 101/271 (37.27%), Postives = 156/271 (57.56%), Query Frame = 0


Query: 25  EPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLI 84
           +P LSK+A K+L +QQ ++A + +K+ + + +KK+               + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKI 88

Query: 85  ESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYC 144
           E+   ++           +KK  RL         IV+D  F  LM   EI+SL QQ+  C
Sbjct: 89  EAGEVVKS----------QKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 145 YAVNGRCPSPCHLWLTGCNGEMETQLQ-RLPG-FDKW-IIEKERRSYIDALQDQKENLVY 204
           ++ N     P  L+ T   G ++T+    L G  + W       +SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 205 LTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQV 264
           L+ADS+  + +LD  KIYI+G +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 268

Query: 265 LTVNQVIEILLKYLETRDWKASFFQVVPQRK 293
           LTVNQV EIL  +LE RDW+ +F +V+P+RK
Sbjct: 269 LTVNQVFEILSLWLEYRDWEKAFMEVIPKRK 273

[Close]

BLAST of PI0023023 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 6.3e-36
Identity = 96/268 (35.82%), Postives = 151/268 (56.34%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++  +K + KE++K                            
Sbjct: 39  PISKRQLKKLMKQKQWEEQREQRKEKRKEKRK---------------------------- 98

Query: 87  RRSLRKERMGKRSEDREKKIERLNTAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 146
           R+ L + ++   S+  ++K  R + A+     +VID  F  LM   +I  L +QI  CYA
Sbjct: 99  RKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCSFDDLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPCHLWLTGCNGEMETQL-QRLPGFDKW-IIEKERRSYIDALQDQKENLVYLTA 206
            N R   P   +LT   G+++  + +   G+  W  I  +   Y + +  +KE+LVYLT+
Sbjct: 159 ENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIHIKSEHYSELI--KKEDLVYLTS 218

Query: 207 DSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTV 266
           DS  VL DLD  K Y++GGLVD +  KG+T K+A   GI+ A+LP+  ++KM+S +VL V
Sbjct: 219 DSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKVLAV 275

Query: 267 NQVIEILLKYLETRDWKASFFQVVPQRK 293
           N V EI+L++LETRDW+ +FF ++PQRK
Sbjct: 279 NHVFEIILEFLETRDWQEAFFTILPQRK 275

[Close]

BLAST of PI0023023 vs. ExPASy Swiss-Prot
Match: Q8TBZ6 (tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 9.1e-35
Identity = 110/329 (33.43%), Postives = 177/329 (53.80%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++     + ++QK+K+ +RKRK+ E                 
Sbjct: 39  PISKRQMKKLIKQKQWEEQR-----ELRKQKRKE-KRKRKKLE----------------- 98

Query: 87  RRSLRKERMGKRSEDREKKIERLNTAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 146
               R+ +M   S+  ++K  R +        ++ID  F HLM   +I  L +QI  CYA
Sbjct: 99  ----RQCQMEPNSDGHDRKRVRRDVVHST-LRLIIDCSFDHLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPCHLWLTGCNGEMETQL-QRLPGFDKW-IIEKERRSYIDALQDQKENLVYLTA 206
            N R   P   +LT   G+++  + +   G+  W  I  +   Y + +  +KE+L+YLT+
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSELI--KKEDLIYLTS 218

Query: 207 DSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTV 266
           DS  +L +LD  K Y++GGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL V
Sbjct: 219 DSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDYGINHAQLPLGNFVKMNSRKVLAV 278

Query: 267 NQVIEILLKYLETRDWKASFFQVVPQRK--------------------RREADSNSDHPL 326
           N V EI+L+YLETRDW+ +FF ++PQRK                      E  S+SD   
Sbjct: 279 NHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDKACESASHDNQSVRMEEGGSDSDSSE 337

Query: 327 DGEENGDGDNKHETKKTKCDEVSSSDHNL 334
           +     + D+ HE K+ K +   S+ ++L
Sbjct: 339 EEYSRNELDSPHEEKQDKENHTESTVNSL 337

[Close]

BLAST of PI0023023 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.6e-33
Identity = 106/320 (33.12%), Postives = 173/320 (54.06%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++     + ++QK+K+ +RKRK+ E +    +  + +     
Sbjct: 39  PISKRQMKKLMKQKQWEEQR-----ELRKQKRKE-KRKRKQLERQCQPESNSDGSDRKRI 98

Query: 87  RRSLRKERMGKRSEDREKKIERLNTAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 146
           RR +    +                       ++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RRDVVHSPL----------------------RLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPCHLWLTGCNGEMETQL-QRLPGFDKW-IIEKERRSYIDALQDQKENLVYLTA 206
            N R   P   +LT   G+++  + +   G+  W  I  +   Y + +  QKE+L+YLT+
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSEFI--QKEDLIYLTS 218

Query: 207 DSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTV 266
           DS  +L +LD  K Y++GGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL V
Sbjct: 219 DSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVLAV 278

Query: 267 NQVIEILLKYLETRDWKASFFQVVPQRK----RREADSNSDHP-----LDGEENGDG--- 326
           N V EI+L+YLETRDW+ +FF ++PQRK      +A  +  H      ++G  N D    
Sbjct: 279 NHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQACESCSHDKKFARVEGGLNSDSSEE 328

Query: 327 DNKHETKKTKCDEVSSSDHN 333
           +N+HE   T  +E    +++
Sbjct: 339 ENRHELDSTHEEEKQDKENS 328

[Close]

BLAST of PI0023023 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 115/347 (33.14%), Postives = 185/347 (53.31%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQ---AKEQK 60
           +EAV E     +DP       N  +   S++A      Q+   A +++ K Q   +K Q 
Sbjct: 3   LEAVTEPGKCSIDP-------NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQM 62

Query: 61  KKDIERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQ 120
           KK +  K+K+WE++     ++ + K    +R L ++   + + D   +    +  +    
Sbjct: 63  KKFL--KQKQWEDQRELRKQKRKEK--RQKRKLERQAQAEHNIDANSRKRFRHEVQPSAL 122

Query: 121 NIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFD 180
            ++ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G++++ +     +D
Sbjct: 123 RLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMDE---YD 182

Query: 181 K-WIIEKERRSYIDALQD--QKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGIT 240
           K WI  K+     +  +D  +KE+LVYLT+DS  VL +LD  K YI+GGLVD N  KGIT
Sbjct: 183 KGWINWKDIHIKPEHYKDLIKKEDLVYLTSDSPEVLSELDETKAYIIGGLVDHNHHKGIT 242

Query: 241 MKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRK-- 300
            KKA E GI  A+LP+G+++KM++ +VL VN V EI+L +LE ++WK +FF V+PQRK  
Sbjct: 243 YKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEWKEAFFSVLPQRKGA 302

Query: 301 ------RREADSNSDHPLDGEENGDGDNKHETKKT-----KCDEVSS 329
                   + +  +    DGE++ D D+  +   T     +C+E +S
Sbjct: 303 IPLTETGEQPECRASEQEDGEDS-DSDSSIDESATIQPSVRCEEQNS 334

[Close]

BLAST of PI0023023 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.2e-166
Identity = 310/333 (93.09%), Postives = 320/333 (96.10%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEE H+ KMDPPC  S CNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEEKLASVTEEERAKLIESRR+LRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTP EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTADSETVLDDLD+KKIYIVGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFF+VVPQRKR   DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVSSSDHNL 334
           DHP+DGEENGDGDNK+ETKKTKCDEVSSSD+NL
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 333

[Close]

BLAST of PI0023023 vs. ExPASy TrEMBL
Match: A0A5D3CW30 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00280 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.3e-164
Identity = 305/327 (93.27%), Postives = 313/327 (95.72%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  HS K+DPPC PS CNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRR+LRKERMGKRSEDREKKIERLNTAKDCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKERRSYIDALQ+QKENLVYLTADSETVLDDLD+KKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFQVVPQRKR  ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVS 328
           DHP+DGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

[Close]

BLAST of PI0023023 vs. ExPASy TrEMBL
Match: A0A1S3CL37 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502202 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.3e-164
Identity = 305/327 (93.27%), Postives = 313/327 (95.72%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  HS K+DPPC PS CNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRR+LRKERMGKRSEDREKKIERLNTAKDCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKERRSYIDALQ+QKENLVYLTADSETVLDDLD+KKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFQVVPQRKR  ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVS 328
           DHP+DGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

[Close]

BLAST of PI0023023 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.6e-159
Identity = 296/331 (89.43%), Postives = 313/331 (94.56%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA+EED S+KMDPPC PS CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWE+ LASVTEEERAKLIESRRSLRKERMGKRSE++EKKIERLN AKDCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQDQKENLVYLTADSETVLDDLD+KKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKR EA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVSSSDH 332
           DH +DGEENGD D++ E+KK KC EVSSS+H
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSNH 331

[Close]

BLAST of PI0023023 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.1e-159
Identity = 296/331 (89.43%), Postives = 312/331 (94.26%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA EED S+KMDPPC PS CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEE LASVTEEERAKLIESR+SLRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQD KENLVYLTADSETVLDDLD+KKIYI+GGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWKASFFQVVPQRKR EA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVSSSDH 332
           DH +DG+ENGD D++ ETKKTKCDEVSSS+H
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSNH 331

[Close]

BLAST of PI0023023 vs. NCBI nr
Match: XP_004139517.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucumis sativus] >KGN65001.1 hypothetical protein Csa_022718 [Cucumis sativus])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 310/333 (93.09%), Postives = 320/333 (96.10%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEE H+ KMDPPC  S CNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEEKLASVTEEERAKLIESRR+LRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTP EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTADSETVLDDLD+KKIYIVGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFF+VVPQRKR   DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVSSSDHNL 334
           DHP+DGEENGDGDNK+ETKKTKCDEVSSSD+NL
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 333

[Close]

BLAST of PI0023023 vs. NCBI nr
Match: XP_008464275.1 (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo] >KAA0042332.1 tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK15458.1 tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 586.3 bits (1510), Expect = 1.7e-163
Identity = 305/327 (93.27%), Postives = 313/327 (95.72%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  HS K+DPPC PS CNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRR+LRKERMGKRSEDREKKIERLNTAKDCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKERRSYIDALQ+QKENLVYLTADSETVLDDLD+KKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFQVVPQRKR  ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVS 328
           DHP+DGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

[Close]

BLAST of PI0023023 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576.2 bits (1484), Expect = 1.8e-160
Identity = 299/331 (90.33%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA+EED S+KMDPPC PS CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KE KKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEE LASVTEEERAKLIESR+SLRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQDQKENLVYLTADSETVLDDLD+KKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKR EA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVSSSDH 332
           DH +DGEENGD D++ ETKKTKCDEVSSS+H
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNH 331

[Close]

BLAST of PI0023023 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 575.5 bits (1482), Expect = 3.0e-160
Identity = 299/331 (90.33%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEA EED S+KMDPPC PS CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWEE LASVTEEERAKLIESR+SLRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQDQKENLVYLTADSETVLDDLD+KKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKR EA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPLDGEENGDGDNKHETKKTKCDEVSSSDH 332
           DH +DGEENGD D++ ETKKTKCDEVSSS+H
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNH 331

[Close]

BLAST of PI0023023 vs. NCBI nr
Match: XP_038893739.1 (tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida])

HSP 1 Score: 573.2 bits (1476), Expect = 1.5e-159
Identity = 304/337 (90.21%), Postives = 315/337 (93.47%), Query Frame = 0


Query: 1   MEAVEEDHSSKMDPPCFPSPCNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEED S+KMDPP  P   NPPEPPLSKNA+KKLLKQQRYEAKKA+KKLQAKEQKKKD
Sbjct: 1   MEAVEEDQSAKMDPPPSPFAFNPPEPPLSKNAKKKLLKQQRYEAKKAEKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120
           IERKRKEWE+ LASVTEEER+KLIESRRSLRKERMGKRSE+REKKIERLN AKDCGQNIV
Sbjct: 61  IERKRKEWEDTLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNRAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKERRSYIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQDQKENLVYLTAD+ETVLDDLD KKIYI+GGLVDRNRWKGITMKKA E
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGITMKKAHE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKR  ADSNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCGADSNS 300

Query: 301 DHPLDGEEN----GDGDNKHETKKTKCDEVSSSDHNL 334
           DHP+D EEN    GDGD++HE KKTKCDEVSSSD  L
Sbjct: 301 DHPVDEEENGDGDGDGDDEHEMKKTKCDEVSSSDDTL 337

[Close]

BLAST of PI0023023 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 402.5 bits (1033), Expect = 3.3e-112
Identity = 222/326 (68.10%), Postives = 261/326 (80.06%), Query Frame = 0


Query: 13  DPPCFPSPCNPP-----EPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKE 72
           D  CF  P + P      PPLSKNA+KK LKQQRYEAKKA+KK Q KE K+K+ ERK KE
Sbjct: 9   DNGCFDKPESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGERKLKE 68

Query: 73  WEEKLASVTEEERAKLIESRRSLRKERMGKRSEDREKKIERLNTAKDCGQNIVIDLEFSH 132
           WEE LA+ TEEER KLIESR+SLRKERM KRSE++EKKIERLN AK+ GQ IV+D++F+H
Sbjct: 69  WEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVDVDFAH 128

Query: 133 LMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWIIEKERRS 192
           LM+ SEI SLVQQIMYCYAVNGR  SPCHLWLTG  G+M T+L +LPGF+KW IEKE R 
Sbjct: 129 LMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIEKESRC 188

Query: 193 YIDALQDQKENLVYLTADSETVLDDLDVKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAK 252
           YI+A+ DQK+NLVYLTADSETVLDDLD K IYI+GGLVDRNR+KGITM KAQEQGI+TAK
Sbjct: 189 YIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQGIKTAK 248

Query: 253 LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRREADSNSDHPLDGE 312
           LPIG Y+KMSSSQVLTVNQV+EIL+K+LETRDWK +FF V+PQRKR   D     P+D  
Sbjct: 249 LPIGEYMKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLD-----PVDCS 308

Query: 313 ENGDGDNKHETKKTKCDEVSSSDHNL 334
           +      +H+ K T+ +E S+  H+L
Sbjct: 309 KLEHISEEHQEKDTEANE-SADKHDL 328

The following BLAST results are available for this feature:

BLAST of PI0023023 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O14214	7.5e-37	37.27	tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 ...	[more]
Q8C1Z8	6.3e-36	35.82	tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=...	[more]
Q8TBZ6	9.1e-35	33.43	tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1	[more]
Q3MHI8	8.6e-33	33.13	tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1	[more]
Q66JJ4	1.1e-32	33.14	tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=...	[more]

BLAST of PI0023023 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYJ3	5.2e-166	93.09	SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3...	[more]
A0A5D3CW30	8.3e-164	93.27	tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CL37	8.3e-164	93.27	tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1I7W5	1.6e-159	89.43	tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1EPG7	6.1e-159	89.43	tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of PI0023023 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139517.1	1.1e-165	93.09	tRNA (guanine(9)-N1)-methyltransferase [Cucumis sativus] >KGN65001.1 hypothetica...	[more]
XP_008464275.1	1.7e-163	93.27	PREDICTED: tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo] >KAA...	[more]
XP_023520714.1	1.8e-160	90.33	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo]	[more]
KAG6583717.1	3.0e-160	90.33	hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038893739.1	1.5e-159	90.21	tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida]	[more]

BLAST of PI0023023 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47680.1	3.3e-112	68.10	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 37..93
None	No IPR available	PIRSR	PIRSR016323-1	PIRSR016323-1	coord: 37..309 e-value: 6.1E-83 score: 276.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..72
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 291..333
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..72
None	No IPR available	PANTHER	PTHR13563:SF17	BNAA06G35330D PROTEIN	coord: 14..326
None	No IPR available	CDD	cd18089	SPOUT_Trm10-like	coord: 117..287 e-value: 2.79511E-83 score: 247.834
IPR016653	tRNA (guanine(9)-N(1))-methyltransferase TRM10/TRM10A	PIRSF	PIRSF016323	tRNA_m1G_Mtase_euk	coord: 2..331 e-value: 1.2E-88 score: 295.1
IPR016009	tRNA methyltransferase TRMD/TRM10-type domain	PFAM	PF01746	tRNA_m1G_MT	coord: 128..292 e-value: 4.8E-29 score: 101.4
IPR038459	tRNA methyltransferase TRM10-type domain superfamily	GENE3D	3.40.1280.30		coord: 97..292 e-value: 2.1E-69 score: 235.0
IPR007356	tRNA (guanine-N1-)-methyltransferase, eukaryotic	PANTHER	PTHR13563	TRNA (GUANINE-9-) METHYLTRANSFERASE	coord: 14..326
IPR028564	tRNA methyltransferase TRM10-type domain	PROSITE	PS51675	SAM_MT_TRM10	coord: 105..295 score: 47.094334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0023023.1	PI0023023.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002939	tRNA N1-guanine methylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0052905	tRNA (guanine(9)-N(1))-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity