PI0022861 (gene) Melon (PI 482460) v1

Overview


Name	PI0022861
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	kunitz trypsin inhibitor 2
Location	chr12: 1865106 .. 1865721 (-)
RNA-Seq Expression	PI0022861
Synteny	PI0022861

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGACGCCTCACCCGATGCCGTCCTCGACACCGATGGAAAGAAGCTTCGAGCCGGTGATCAATACTACATTCTCTCAGTTTACAGCAGAAACAGCGGCGGATTAAGCATCGGTGGTATCTACGGGTACGAAAAATGTCCAATCAACATCCTCCCCGAATCTTACGAATATTTACACGGTCTTCCGGCGATATTTTCCCCCGTAAACCCTAAAAAAGGTGTGGTCCGAGTCTCGACCGATTTGAACATCCAATTCGAGGCGAACACAAGGTGCGGAATATCGACCGTGTGGAAAGTAGGTAATTTGATCAATACTTGAAGCAGTATTTCGTGACGATGGGCGGAACGAAAGGGAATCCGGGGCGGGAAACGATCGAAAATTGGTTCAAAGTAGAGAAGTATGGTAAGAATTATAAGTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAATGTGCAAAGATGTGGGAATATTTGTGAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAAAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGACGCCTCACCCGATGCCGTCCTCGACACCGATGGAAAGAAGCTTCGAGCCGGTGATCAATACTACATTCTCTCAGTTTACAGCAGAAACAGCGGCGGATTAAGCATCGGTGGTATCTACGGGTACGAAAAATGTCCAATCAACATCCTCCCCGAATCTTACGAATATTTACACGGTCTTCCGGCGATATTTTCCCCCGTAAACCCTAAAAAAGGTGTGGTCCGAGTCTCGACCGATTTGAACATCCAATTCGAGGCGAACACAAGGTGCGGAATATCGACCGTGTGGAAATATTTCGTGACGATGGGCGGAACGAAAGGGAATCCGGGGCGGGAAACGATCGAAAATTGGTTCAAAGTAGAGAAGTATGTTTGTAAGTATTGCAAAGTAATGTGCAAAGATGTGGGAATATTTGTGAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAAAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGACGCCTCACCCGATGCCGTCCTCGACACCGATGGAAAGAAGCTTCGAGCCGGTGATCAATACTACATTCTCTCAGTTTACAGCAGAAACAGCGGCGGATTAAGCATCGGTGGTATCTACGGGTACGAAAAATGTCCAATCAACATCCTCCCCGAATCTTACGAATATTTACACGGTCTTCCGGCGATATTTTCCCCCGTAAACCCTAAAAAAGGTGTGGTCCGAGTCTCGACCGATTTGAACATCCAATTCGAGGCGAACACAAGGTGCGGAATATCGACCGTGTGGAAATATTTCGTGACGATGGGCGGAACGAAAGGGAATCCGGGGCGGGAAACGATCGAAAATTGGTTCAAAGTAGAGAAGTATGTTTGTAAGTATTGCAAAGTAATGTGCAAAGATGTGGGAATATTTGTGAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGA

Protein sequence

MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVWKYFVTMGGTKGNPGRETIENWFKVEKYVCKYCKVMCKDVGIFVKNGRRVIALNDAPFPVMFKKV

Homology

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BLAST of PI0022861 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 151.4 bits (381), Expect = 1.0e-35
Identity = 90/216 (41.67%), Postives = 117/216 (54.17%), Query Frame = 0


Query: 1   MKNFALLCFLF-----IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNS 60
           MK   +L   F     ++ A++      AD++P+ VLD DG+KLR G  YYI+ V   + 
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLSIGGI--YGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTR 120
           GGL++      G   CP  ++    E  H  P  F P NPK+ VVRVSTDLNI F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--GISTVWK----------YFVTMGGTKGNPGRETIENWFKVEKY-------------V 180
           C    STVW+          YFVT+GG KGNPG ETI +WFK+E++             V
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CKYCKVMCKDVGIFV-KNGRRVIALNDAPFPVMFKK 184
           C  CKV C DVGI++ + GRR +AL+D PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of PI0022861 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.9e-32
Identity = 72/193 (37.31%), Postives = 115/193 (59.59%), Query Frame = 0


Query: 6   LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYG 65
           L  FL + +  S  R    +A+ + V D +GK L  G  YYIL V     GGL++  +  
Sbjct: 5   LYIFLLLAVFISH-RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNL-K 64

Query: 66  YEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGIS----TVWK 125
            E CP +++ + +E   GLP  FSP + K   + VSTD+NI+F   +   ++    T  +
Sbjct: 65  TETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSIWELANFDETTKQ 124

Query: 126 YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDVGIFVKNGRRVI 184
           +F++  G +GNPG++T++NWFK++K+           VC +CKV+C+DVG+FV++G+R +
Sbjct: 125 WFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRL 184

[Close]

BLAST of PI0022861 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.5e-26
Identity = 76/209 (36.36%), Postives = 106/209 (50.72%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYIL-SVYSRNSGGLS 60
           MK    +  L     S    F  A+A+   VLDTDG +L+ G QYY+L S+     GGL+
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLA 60

Query: 61  IGGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFE--ANTRCGIS 120
           +G   G + CP  ++    +  +G P IFS  + K  VVRVSTD+NI+F    +  C  S
Sbjct: 61  LGRATG-QSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTS 120

Query: 121 TVW----------KYFVTMGGTKGNPGRETIENWFKVEK------------YVCKYCKVM 180
           TVW          K++VT  G KG PG  T+ +WFK+EK             VC  C  +
Sbjct: 121 TVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTL 180

Query: 181 CKDVGIFV-KNGRRVIALNDAPFPVMFKK 184
           C D+G     +G+  +AL+D  +  MFKK
Sbjct: 181 CSDIGRHSDDDGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of PI0022861 vs. ExPASy Swiss-Prot
Match: P16347 (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 58/178 (32.58%), Postives = 87/178 (48.88%), Query Frame = 0


Query: 28  PDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYGYEKCPINILPESYEYLHGLPAI 87
           P  V DTDG +LRA   YY+L     + GGL++   +G  +CP+ +  E+     GLP  
Sbjct: 2   PPPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHG-RRCPLFVSQEADGQRDGLPVR 61

Query: 88  FSPVN--PKKGVVRVSTDLNIQFEANTRCGISTVW----------KYFVTMGGTKGNPGR 147
            +P    P   ++R+STD+ I F A T C  ST W          ++ +T  G   +P  
Sbjct: 62  IAPHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDSELVSGRRHVIT--GPVRDPSP 121

Query: 148 ETIENWFKVEKY----VCKY----CKVMCKDVGIF--VKNGRRVIALNDAPFPVMFKK 184
              EN F++EKY    V +Y    C   C+D+G+F  +K G   +   +    V+FKK
Sbjct: 122 SGRENAFRIEKYSGAEVHEYKLMACGDSCQDLGVFRDLKGGAWFLGATEPYHVVVFKK 176

[Close]

BLAST of PI0022861 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 2.6e-15
Identity = 58/176 (32.95%), Postives = 82/176 (46.59%), Query Frame = 0


Query: 26  ASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYGYEKCPINILPESYEYLHGLP 85
           A+P  V DT+G +L A   YY+L     + GGL++        CP+ +  E+ E   G P
Sbjct: 22  AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAP--RVLPCPLLVAQETDERRKGFP 81

Query: 86  AIFSP----VNPKKGVVRVSTDLNIQFEANTRCGISTVW----------KYFVTMGGTKG 145
             F+P      P+   +RVSTD+ I+F A T C  ST W          +  VT  G   
Sbjct: 82  VRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDEPLTGARRVVT--GPLI 141

Query: 146 NPGRETIENWFKVEKYVCKY----CKVMCKDVGIFVKNGRRVIALNDAPFPVMFKK 184
            P     EN F+VEKY   Y    C+  C+D+G+     R  +  +  P  V+FKK
Sbjct: 142 GPSPSGRENAFRVEKYGGGYKLVSCRDSCQDLGVSRDGARAWLGASQPPHVVVFKK 193

[Close]

BLAST of PI0022861 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.5e-95
Identity = 176/205 (85.85%), Postives = 181/205 (88.29%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           M+NFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESY+YLHGLPA FSP+NPKKGVVRVSTDLNIQFEANTRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120

Query: 121 K----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDV 180
           K          YFVTMGG KGNPGRETIENWFKVEKY           VCKYCKV+CKDV
Sbjct: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180

Query: 181 GIFVKNGRRVIALNDAPFPVMFKKV 185
           G+F KNGRRVIALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of PI0022861 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.2e-93
Identity = 173/205 (84.39%), Postives = 179/205 (87.32%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESY+YL GLPA FSPVNPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 K----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDV 180
           K          YFVTMGGTKGNPGRETI NWFKVEK+           VCKYCKVMCKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GIFVKNGRRVIALNDAPFPVMFKKV 185
           G+F KNGRR+ ALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of PI0022861 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.2e-93
Identity = 173/205 (84.39%), Postives = 179/205 (87.32%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESY+YL GLPA FSPVNPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 K----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDV 180
           K          YFVTMGGTKGNPGRETI NWFKVEK+           VCKYCKVMCKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GIFVKNGRRVIALNDAPFPVMFKKV 185
           G+F KNGRR+ ALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of PI0022861 vs. ExPASy TrEMBL
Match: A0A6J1C8C4 (kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.2e-67
Identity = 126/205 (61.46%), Postives = 157/205 (76.59%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MKNFALLCFLF +IAS+EVR C++DASPDAVLD DGKKLRAG  YYIL V     GGL++
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           G I G++KCP++++ E +E  +GLPA F+PVNPKKGVVRVSTDLN++FEA+T C  STVW
Sbjct: 61  GNI-GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTVW 120

Query: 121 K----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDV 180
           K          + V +GG +GNPG+ET++NWFK+EK+           VCK+CKV+CKDV
Sbjct: 121 KLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKDV 180

Query: 181 GIFVKNGRRVIALNDAPFPVMFKKV 185
           GIF+KNG R +AL+D PFPVMFKKV
Sbjct: 181 GIFIKNGNRALALSDTPFPVMFKKV 204

[Close]

BLAST of PI0022861 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.8e-64
Identity = 133/210 (63.33%), Postives = 151/210 (71.90%), Query Frame = 0


Query: 1   MKNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60
           MK FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LSIGGI-YGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGI 120
           L+IG I   Y++C IN++ E YE   G P  F P+NPKKGVVRVSTDLNI+F+A TRC  
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWK----------YFVTMGGTKGNPGRETIENWFKVEKY------------VCKYCKV 180
           STVWK          YFVT+GGTKGNPGRET ENWFK+EKY            VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDVGIFVKNGRRVIALNDAPFPVMFKKV 185
           MCKD+GIF  NG R + L+D PFPV+FKKV
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of PI0022861 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 350.9 bits (899), Expect = 6.7e-93
Identity = 173/205 (84.39%), Postives = 179/205 (87.32%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESY+YL GLPA FSPVNPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 K----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDV 180
           K          YFVTMGGTKGNPGRETI NWFKVEK+           VCKYCKVMCKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GIFVKNGRRVIALNDAPFPVMFKKV 185
           G+F KNGRR+ ALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of PI0022861 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 320.1 bits (819), Expect = 1.3e-83
Identity = 160/206 (77.67%), Postives = 170/206 (82.52%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MKNFALL FLFIVI SSEVRFCRAD SPDAVLDTDGKKLR+ DQYYILSVYSRNSGGLSI
Sbjct: 1   MKNFALLSFLFIVIVSSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVYSRNSGGLSI 60

Query: 61  GGI-YGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTV 120
           G I  G EKCPINILPESYEYLHGL A F P+NPKKGVVRVSTDLNIQFEA+TRCG STV
Sbjct: 61  GSIQQGKEKCPINILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGESTV 120

Query: 121 WK----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKD 180
           WK          YFVT+GG KGNPGRET+ NWFK+EKY           VCKYCKV+CKD
Sbjct: 121 WKIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGNNYKLVHCPTVCKYCKVICKD 180

Query: 181 VGIFVKNGRRVIALNDAPFPVMFKKV 185
           VGIF KNG+RV+ALND+PFPVMFKKV
Sbjct: 181 VGIFYKNGKRVLALNDSPFPVMFKKV 206

[Close]

BLAST of PI0022861 vs. NCBI nr
Match: XP_038893656.1 (miraculin-like [Benincasa hispida])

HSP 1 Score: 269.2 bits (687), Expect = 2.6e-68
Identity = 135/209 (64.59%), Postives = 157/209 (75.12%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVR--FCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGL 60
           MK+F LL  +FIVI +S  R  FCR +ASP+A+LDT+GKKLR GD YYIL  +SRN+GGL
Sbjct: 1   MKSFELLSLIFIVIVASTERQQFCRVNASPEAILDTNGKKLRGGDHYYILPFFSRNTGGL 60

Query: 61  SIGGI---YGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCG 120
           ++G I   Y  E CP+N++PE YEY +GLP  FSPVNPKKGV+RVSTDLNIQFE NT C 
Sbjct: 61  ALGNIQREYSNE-CPLNVVPEPYEYFNGLPTTFSPVNPKKGVIRVSTDLNIQFERNTMCA 120

Query: 121 ISTVWK-------YFVTMGGTKGNPGRETIENWFKVEKY-------------VCKYCKVM 180
            STVWK       Y VT+GGTKG+PG ET+ENWFK+EKY             VCKYCKVM
Sbjct: 121 KSTVWKISKFEQQYLVTIGGTKGDPGPETMENWFKIEKYNNNNIYKFVYCPTVCKYCKVM 180

Query: 181 CKDVGIFVKNGRRVIALNDAPFPVMFKKV 185
           CKDVGIFVKNGR  +AL+DAPFPVMFKKV
Sbjct: 181 CKDVGIFVKNGRMALALSDAPFPVMFKKV 208

[Close]

BLAST of PI0022861 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 263.8 bits (673), Expect = 1.1e-66
Identity = 126/205 (61.46%), Postives = 157/205 (76.59%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MKNFALLCFLF +IAS+EVR C++DASPDAVLD DGKKLRAG  YYIL V     GGL++
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GGIYGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           G I G++KCP++++ E +E  +GLPA F+PVNPKKGVVRVSTDLN++FEA+T C  STVW
Sbjct: 61  GNI-GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTVW 120

Query: 121 K----------YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDV 180
           K          + V +GG +GNPG+ET++NWFK+EK+           VCK+CKV+CKDV
Sbjct: 121 KLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKDV 180

Query: 181 GIFVKNGRRVIALNDAPFPVMFKKV 185
           GIF+KNG R +AL+D PFPVMFKKV
Sbjct: 181 GIFIKNGNRALALSDTPFPVMFKKV 204

[Close]

BLAST of PI0022861 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 255.4 bits (651), Expect = 3.8e-64
Identity = 133/210 (63.33%), Postives = 151/210 (71.90%), Query Frame = 0


Query: 1   MKNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60
           MK FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LSIGGI-YGYEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGI 120
           L+IG I   Y++C IN++ E YE   G P  F P+NPKKGVVRVSTDLNI+F+A TRC  
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWK----------YFVTMGGTKGNPGRETIENWFKVEKY------------VCKYCKV 180
           STVWK          YFVT+GGTKGNPGRET ENWFK+EKY            VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDVGIFVKNGRRVIALNDAPFPVMFKKV 185
           MCKD+GIF  NG R + L+D PFPV+FKKV
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of PI0022861 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 138.3 bits (347), Expect = 6.3e-33
Identity = 72/193 (37.31%), Postives = 115/193 (59.59%), Query Frame = 0


Query: 6   LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYG 65
           L  FL + +  S  R    +A+ + V D +GK L  G  YYIL V     GGL++  +  
Sbjct: 5   LYIFLLLAVFISH-RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNL-K 64

Query: 66  YEKCPINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANTRCGIS----TVWK 125
            E CP +++ + +E   GLP  FSP + K   + VSTD+NI+F   +   ++    T  +
Sbjct: 65  TETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSIWELANFDETTKQ 124

Query: 126 YFVTMGGTKGNPGRETIENWFKVEKY-----------VCKYCKVMCKDVGIFVKNGRRVI 184
           +F++  G +GNPG++T++NWFK++K+           VC +CKV+C+DVG+FV++G+R +
Sbjct: 125 WFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRL 184

[Close]

BLAST of PI0022861 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 90.1 bits (222), Expect = 2.0e-18
Identity = 64/178 (35.96%), Postives = 90/178 (50.56%), Query Frame = 0


Query: 30  AVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYGYEKCPINILPESYEYLHGLPAIFS 89
           AV+D DG  +   + YY+L V     GGL++ G  G + CP +I+ ES E   G+P  FS
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG-RGGQPCPYDIVQESSEVDEGIPVKFS 88

Query: 90  PVNPKKGVVRVSTDLNIQFEAN-TRCGISTVWK----------YFVTMGGTKGNPGRETI 149
               K   V  S +LNI+ +   T C  ST W+          YFV  G      G++++
Sbjct: 89  NWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSL 148

Query: 150 ENWFKVEK-----YVCKYC-------KVMCKDVGIFVKN-GRRVIALNDAPFPVMFKK 184
           +++FK+EK     Y   +C          C DVGIF+   G R +AL+D PF VMFKK
Sbjct: 149 KSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of PI0022861 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 74.7 bits (182), Expect = 8.6e-14
Identity = 61/207 (29.47%), Postives = 98/207 (47.34%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASP-DAVLDTDGKKLRAGDQYYILSVYSRNSGGL- 60
           M+   L      V+++       ADA+P   VLD  G  +++  QYYI+       GGL 
Sbjct: 1   MEKLTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLI 60

Query: 61  -SIGGIYGYEKC-PINILPESYEYLHGLPAIFSPVNPKKGVVRVSTDLNIQFEANT-RCG 120
            S   +   + C  ++I+  S  ++ GLP  FSP+N K   V++S  LN++F++    C 
Sbjct: 61  PSNRNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCP 120

Query: 121 ISTVW---------KYFVTMGGTKGNPGRETIENWFKVEK----YVCKYCK----VMCKD 180
            S VW         K FV++GG KG        +WF++++    Y   YC     V C +
Sbjct: 121 DSKVWRIDHSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPISSIVACIN 180

Query: 181 VGIFVKNG--RRVIALNDAPFPVMFKK 184
           V + + +   RR++   D  F V F+K
Sbjct: 181 VSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of PI0022861 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13087	1.0e-35	41.67	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q9LMU2	8.9e-32	37.31	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P32765	9.5e-26	36.36	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
P16347	1.5e-15	32.58	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ...	[more]
P29421	2.6e-15	32.95	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of PI0022861 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT81	1.5e-95	85.85	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]
A0A5D3BJ02	3.2e-93	84.39	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	3.2e-93	84.39	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]
A0A6J1C8C4	5.2e-67	61.46	kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 S...	[more]
A0A5D3BJ25	1.8e-64	63.33	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]

BLAST of PI0022861 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437082.1	6.7e-93	84.39	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_038895396.1	1.3e-83	77.67	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]
XP_038893656.1	2.6e-68	64.59	miraculin-like [Benincasa hispida]	[more]
XP_022137472.1	1.1e-66	61.46	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_008437071.1	3.8e-64	63.33	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]

BLAST of PI0022861 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	6.3e-33	37.31	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	2.0e-18	35.96	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	8.6e-14	29.47	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 69..89 score: 20.35 coord: 30..59 score: 39.61
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..184 e-value: 2.4E-39 score: 146.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..183 e-value: 1.2E-39 score: 136.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 6..184
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..183 e-value: 2.8175E-46 score: 147.874
None	No IPR available	GENE3D	2.80.10.50		coord: 25..184 e-value: 1.2E-48 score: 167.0
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 6..184
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0022861.1	PI0022861.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity