PI0022781 (gene) Melon (PI 482460) v1

Overview


Name	PI0022781
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Ribonuclease T(2)
Location	chr04: 12823710 .. 12824500 (-)
RNA-Seq Expression	PI0022781
Synteny	PI0022781

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAACATCTTTTCACTTTCTGTCTTTTAAGTTTGTTGTTGGTTGTGAATGGCGATCCATTTGACTTCTTCCAACTAGTTGAACAATGGGGTCCAAACACATGTAATAACACTCTTGGCACCCAGTGTCATGCTCAACCTCAACCCATGTTCACTATTCATGGACTATGGCCGAGTAACTTTTCAAATGCCCGTCTTTCTTGTGTAGTTCATAGAAAATTTAATCCTCAAATGGTTCCCATCTTCTTCTATTATTTTTTTTTTATGTGTTTTTTCTATCATTAGCATTTTAAACATTGTTTCAACATTCTTTTATTTTATTTTAGGTTACTGTACTTGAACAAGATCTAAATACATATTGGCCAAATGTAATAAGGGGGAACAACTCCGACTTTTGGGGATATGAATGGAGAAAGCATGGGGCATGTACAAACCCTCCTTATACCATACGTCAATATTTTCAAATTACTTTGAATCTCCGAAAGAATAAAAATTATGATCTGTTGGCAATCTTAAATGTTGCTGGGCTCAACCCCCAAATGCCCAAAAATCTCGTATCTCAAGACATCCTAAATCCCATTAAAAATGCAATAGGAAAGAATCCAGGCATCCATTGCAACAACAATGGTATAACTGGGAAGTTGCAATTGTATGAAATTGTACTGTGTTTTGACAAGAATCCTGCTACCCTCATTGACTGCCCTCGGTTTGTTAGCAACAGTTGTCCATCTACATTTGTGTGGCTCCTCCCACAACAGAGTTCAATTGGTGTTCCAGATTATCCATGA

mRNA sequence

ATGGCTCAACATCTTTTCACTTTCTGTCTTTTAAGTTTGTTGTTGGTTGTGAATGGCGATCCATTTGACTTCTTCCAACTAGTTGAACAATGGGGTCCAAACACATGTAATAACACTCTTGGCACCCAGTGTCATGCTCAACCTCAACCCATGTTCACTATTCATGGACTATGGCCGAGTAACTTTTCAAATGCCCGTCTTTCTTGTGTAGTTCATAGAAAATTTAATCCTCAAATGGTTACTGTACTTGAACAAGATCTAAATACATATTGGCCAAATGTAATAAGGGGGAACAACTCCGACTTTTGGGGATATGAATGGAGAAAGCATGGGGCATGTACAAACCCTCCTTATACCATACGTCAATATTTTCAAATTACTTTGAATCTCCGAAAGAATAAAAATTATGATCTGTTGGCAATCTTAAATGTTGCTGGGCTCAACCCCCAAATGCCCAAAAATCTCGTATCTCAAGACATCCTAAATCCCATTAAAAATGCAATAGGAAAGAATCCAGGCATCCATTGCAACAACAATGGTATAACTGGGAAGTTGCAATTGTATGAAATTGTACTGTGTTTTGACAAGAATCCTGCTACCCTCATTGACTGCCCTCGGTTTGTTAGCAACAGTTGTCCATCTACATTTGTGTGGCTCCTCCCACAACAGAGTTCAATTGGTGTTCCAGATTATCCATGA

Coding sequence (CDS)

ATGGCTCAACATCTTTTCACTTTCTGTCTTTTAAGTTTGTTGTTGGTTGTGAATGGCGATCCATTTGACTTCTTCCAACTAGTTGAACAATGGGGTCCAAACACATGTAATAACACTCTTGGCACCCAGTGTCATGCTCAACCTCAACCCATGTTCACTATTCATGGACTATGGCCGAGTAACTTTTCAAATGCCCGTCTTTCTTGTGTAGTTCATAGAAAATTTAATCCTCAAATGGTTACTGTACTTGAACAAGATCTAAATACATATTGGCCAAATGTAATAAGGGGGAACAACTCCGACTTTTGGGGATATGAATGGAGAAAGCATGGGGCATGTACAAACCCTCCTTATACCATACGTCAATATTTTCAAATTACTTTGAATCTCCGAAAGAATAAAAATTATGATCTGTTGGCAATCTTAAATGTTGCTGGGCTCAACCCCCAAATGCCCAAAAATCTCGTATCTCAAGACATCCTAAATCCCATTAAAAATGCAATAGGAAAGAATCCAGGCATCCATTGCAACAACAATGGTATAACTGGGAAGTTGCAATTGTATGAAATTGTACTGTGTTTTGACAAGAATCCTGCTACCCTCATTGACTGCCCTCGGTTTGTTAGCAACAGTTGTCCATCTACATTTGTGTGGCTCCTCCCACAACAGAGTTCAATTGGTGTTCCAGATTATCCATGA

Protein sequence

MAQHLFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPSNFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYTIRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNNGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVWLLPQQSSIGVPDYP

Homology

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BLAST of PI0022781 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 6.6e-32
Identity = 67/197 (34.01%), Postives = 100/197 (50.76%), Query Frame = 0


Query: 22  FDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPSNFSNARLSCVVHRKFNPQMVT 81
           FD F  V+QW P  C+      C       FTIHGLWP     +  +C     F+   ++
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 82  VLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYTIRQYFQITLNLRKNKNYDLLAI 141
            L+  LNT WPNV+R NN  FW +EW KHG C+   +    YF++ +++R   NYD++  
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMR--NNYDIIGA 120

Query: 142 LNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNNGITGKLQLYEIVLCFDKNPATL 201
           L      P   +    Q I   +K   GK PG+ C  +  T    L ++V CF ++ +TL
Sbjct: 121 LRPHAAGPN-GRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTL 180

Query: 202 IDCPRFVSNSCPSTFVW 219
           IDC R   ++C + F++
Sbjct: 181 IDCTR---DTCGANFIF 191

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BLAST of PI0022781 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 3.5e-25
Identity = 73/220 (33.18%), Postives = 112/220 (50.91%), Query Frame = 0


Query: 8   FCLLSLLLVVN--------GDPFDFFQLVEQWGPNTCNNTLGTQCH---AQPQPMFTIHG 67
           F L  +LL++            FDFF  V+QW  + C +T  + C+    +P   F IHG
Sbjct: 7   FSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYC-DTKQSCCYPTTGKPAADFGIHG 66

Query: 68  LWPSNFSNARLS-CVVHRKFNPQMVTVLEQDLNTYWPNVI--RGNNSDFWGYEWRKHGAC 127
           LWP+N      S C  +  ++   ++ L   +   WP +    G+ S FW +EW KHG C
Sbjct: 67  LWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTC 126

Query: 128 TNPPYTIRQ-YFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNP 187
                T +  YF+  L+L+     DLL+IL  A ++P   ++    +I N IK+AIG  P
Sbjct: 127 AESVLTNQHAYFKKALDLK--NQIDLLSILQGADIHPD-GESYDLVNIRNAIKSAIGYTP 186

Query: 188 GIHCNNNGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSC 213
            I CN +  +G  QLY++ +C D + ++LI+CP F    C
Sbjct: 187 WIQCNVDQ-SGNSQLYQVYICVDGSGSSLIECPIFPGGKC 221

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BLAST of PI0022781 vs. ExPASy Swiss-Prot
Match: Q38717 (Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-24
Identity = 65/189 (34.39%), Postives = 91/189 (48.15%), Query Frame = 0


Query: 23  DFFQLVEQWGPNTCNNTLGTQCHAQPQPM-FTIHGLWPSNFSNARLSCVVHRKFNPQMVT 82
           D+ +LV QW  + C      +C   P P  FTIHGLWP N++           F     T
Sbjct: 34  DYLKLVLQWPKSFCLIN-SRKCQRNPLPSNFTIHGLWPDNYTRQAPQSCTTNNFQRFTDT 93

Query: 83  VLEQDLNTYWPNV----IRGNNSDFWGYEWRKHGACTNPPYTIRQYFQITLNLRKNKNYD 142
            +EQ +   WP++    I G + +FW  +WRKHG+C  PP+    YF   L L+     D
Sbjct: 94  DIEQRMEESWPDLKQQSIAGLSYNFWQDQWRKHGSCCFPPHESEIYFLKALELK--DRLD 153

Query: 143 LLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNNGITGKLQLYEIVLCFDKN 202
           +L IL     NP  P+      + N I  AIGK P + C       +  L E+V+C D N
Sbjct: 154 VLTILENNNFNPGTPQPFSVLRVFNTISRAIGKTPILKC------AQSYLKEVVICVDNN 213

Query: 203 PATLIDCPR 207
            A+++ CPR
Sbjct: 214 GASVVHCPR 213

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BLAST of PI0022781 vs. ExPASy Swiss-Prot
Match: O80323 (Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-24
Identity = 73/205 (35.61%), Postives = 102/205 (49.76%), Query Frame = 0


Query: 5   LFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPSNFSN 64
           +  F L+ L+L  +   +D+FQ  +Q+    CN+   T C   P  +FT+HGLWPSN   
Sbjct: 6   MMVFLLIVLILCSSTVGYDYFQFTQQYQLAVCNSN-RTLCKDPPDKLFTVHGLWPSNMVG 65

Query: 65  ARLS-CVVHRKFNPQMVTVLEQDLNTYWPNVI-RGNNSDFWGYEWRKHGACTNPPY-TIR 124
              S C +  K   +   +LE  L   WPNV  R  N+ FW  EW KHG+C  P      
Sbjct: 66  PDPSKCPI--KNIRKREKLLEHQLEIIWPNVFDRTKNNLFWDKEWMKHGSCGYPTIDNEN 125

Query: 125 QYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKN-AIGKNPGIHCNNNG 184
            YF+  + +  +K  ++  IL+ A + P   K  +  DI N I+N A  K P + C   G
Sbjct: 126 HYFETVIKMYISKKQNVSRILSKAKIEPDGKKRAL-LDIENAIRNGADNKKPKLKCQKKG 185

Query: 185 ITGKLQLYEIVLCFDKNPATLIDCP 206
            T   +L EI LC DK+    IDCP
Sbjct: 186 TT--TELVEITLCSDKSGEHFIDCP 204

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BLAST of PI0022781 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 8.6e-24
Identity = 65/203 (32.02%), Postives = 97/203 (47.78%), Query Frame = 0


Query: 8   FCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPSNFSNARL 67
           F L+ L+L  +   +D+FQ  +Q+ P  CN+   T C   P  +FT+HGLWPSN +    
Sbjct: 14  FSLIVLILSSSTVGYDYFQFTQQYQPAVCNSK-PTPCKDPPDKLFTVHGLWPSNLNGPHP 73

Query: 68  SCVVHRKFNPQMVTVLEQDLNTYWPNVI-RGNNSDFWGYEWRKHGACTNPP-YTIRQYFQ 127
               +   NP  +  ++  L   WPNV+ R N+  FW  +W KHG+C  P       YFQ
Sbjct: 74  ENCTNATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIMNDTHYFQ 133

Query: 128 ITLNLRKNKNYDLLAILNVAGLNP---QMPKNLVSQDILNPIKNAIGKNPGIHCNNNGIT 187
             +N+   +  ++  IL+ A + P   Q P   +   I N   N   K P   C  N  +
Sbjct: 134 TVINMYITQKQNVSEILSKAKIEPLGIQRPLVHIENAIRNSTNN---KKPKFKCQKN--S 193

Query: 188 GKLQLYEIVLCFDKNPATLIDCP 206
           G  +L E+ LC D +     +CP
Sbjct: 194 GVTELVEVGLCSDGSLTQFRNCP 210

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BLAST of PI0022781 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.4e-85
Identity = 159/234 (67.95%), Postives = 178/234 (76.07%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPS 60
           MAQH F  CLLSLLL VNG PFDFFQLVEQWGPNTCN+  GTQCHAQPQP FTIHGLWPS
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND--GTQCHAQPQPEFTIHGLWPS 60

Query: 61  NFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYTI 120
           NFSNARL C   + F+   +++LE DL+ YWP+VI GNN  FW  EW+KHGAC +PP+ I
Sbjct: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120

Query: 121 RQYFQITLNLRKNKNYDLLAILN-VAGLNPQMPKN-LVSQDILNPIKNAIGKNPGIHCNN 180
            QYFQ++L+   ++ YDLL ILN V   N QMP N L  +DILNPI NA  K  GI CN 
Sbjct: 121 TQYFQLSLD--HHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSC-PSTFVWLLPQQSSIGVPDY 232
           NGIT KLQL+EIVLCFD N  TLIDCP+FVSNSC    FVWLLPQQSS GVPDY
Sbjct: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

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BLAST of PI0022781 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.6e-84
Identity = 155/235 (65.96%), Postives = 178/235 (75.74%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPS 60
           MAQH+F  CLLS LL VNG PFDFFQLV+QWGPN C +  G++C+AQPQPMFTIHGLWPS
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNIC-SPAGSKCYAQPQPMFTIHGLWPS 60

Query: 61  NFSNARLSCVVHRKFNPQ-MVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYT 120
           NFS   + C   +K  PQ  VT LEQ LN YWP+VI G NS+FW +EWRKH  C +PP+T
Sbjct: 61  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFT 120

Query: 121 IRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNN 180
           I  YF I+L  R NK YDLL ILN AGL+ Q  K+L+SQ++L+PIKNA G  PGI CN N
Sbjct: 121 IFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNIN 180

Query: 181 GITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPS--TFVWLLPQQSSIGVPDYP 233
           GIT KLQL EIVLCF  +  TLIDCP FVSN+C S   FVWLLPQQSS+GVPDYP
Sbjct: 181 GITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of PI0022781 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 2.0e-60
Identity = 120/220 (54.55%), Postives = 144/220 (65.45%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGD-PFDFFQLVEQWGPNTCN-NTLGTQCHAQPQPMFTIHGLW 60
           M Q +     LSL++ VNG  PF+FFQ VEQWGPN CN     + CH  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPY 120
           PSN SNA L C    K     V  LE  LN  WP++  GNN  FWG+EW  HG C++PP+
Sbjct: 61  PSNHSNAHLLC--QSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPF 120

Query: 121 TIRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNN 180
           ++ QYFQI+LNL   + +DLL IL  AGLNPQ  +NL  Q+I+ PI+    K PGI CN 
Sbjct: 121 SLFQYFQISLNLL--RKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVW 219
           N  TGK QL EIVLC +K+ ATLIDCP FVSN+C  +FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0022781 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.7e-59
Identity = 114/221 (51.58%), Postives = 140/221 (63.35%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVN--GDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLW 60
           MAQ  F   +   +L+ +     FD+FQ+V+QW P TC+   G +C+A P  MFTIHGLW
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS---GVRCYANPPAMFTIHGLW 60

Query: 61  PSNFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPY 120
           PSN+SN  L C   + FNP  +  L   LNTYWP+VI GNN  FW +EW  HG C++PP+
Sbjct: 61  PSNYSNVPLVC-AGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPF 120

Query: 121 TIRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNN 180
              QYFQ TLN+R N NYDLLAILN AGL P          I   I+ A GK PG+ CN 
Sbjct: 121 NQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVWL 220
           N  + K QL+EI+LCFDKN  TLIDC +F   +CPS FVWL
Sbjct: 181 NAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWL 217

[Close]

BLAST of PI0022781 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.8e-59
Identity = 113/215 (52.56%), Postives = 141/215 (65.58%), Query Frame = 0


Query: 5   LFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPSNFSN 64
           +F F  + L+L VN   FD+FQ+V+QW P  C +++  +CH QP  MFTIHGLWPSN+SN
Sbjct: 10  IFNFVFVVLILCVNAQ-FDYFQMVQQWPPAKC-SSVKVRCHVQPPTMFTIHGLWPSNYSN 69

Query: 65  ARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYTIRQYF 124
           ARL C   + FNP  +  L   LNTYWP+VI+G N  FW +EW +HG C+NPP+   QYF
Sbjct: 70  ARLVC-APQPFNPTQIAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYF 129

Query: 125 QITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNNGIT-G 184
           QITLN+R N +YDLL ILN AGL P        +DI   I+ A GK PG+ CN N  +  
Sbjct: 130 QITLNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKE 189

Query: 185 KLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVW 219
           K QL EI+LCFDK+  TLIDC  F   +CP  FVW
Sbjct: 190 KKQLLEIILCFDKDGITLIDCTEFAGITCPPQFVW 221

[Close]

BLAST of PI0022781 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 325.1 bits (832), Expect = 4.9e-85
Identity = 159/234 (67.95%), Postives = 178/234 (76.07%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPS 60
           MAQH F  CLLSLLL VNG PFDFFQLVEQWGPNTCN+  GTQCHAQPQP FTIHGLWPS
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND--GTQCHAQPQPEFTIHGLWPS 60

Query: 61  NFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYTI 120
           NFSNARL C   + F+   +++LE DL+ YWP+VI GNN  FW  EW+KHGAC +PP+ I
Sbjct: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120

Query: 121 RQYFQITLNLRKNKNYDLLAILN-VAGLNPQMPKN-LVSQDILNPIKNAIGKNPGIHCNN 180
            QYFQ++L+   ++ YDLL ILN V   N QMP N L  +DILNPI NA  K  GI CN 
Sbjct: 121 TQYFQLSLD--HHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSC-PSTFVWLLPQQSSIGVPDY 232
           NGIT KLQL+EIVLCFD N  TLIDCP+FVSNSC    FVWLLPQQSS GVPDY
Sbjct: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of PI0022781 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 5.5e-84
Identity = 155/235 (65.96%), Postives = 178/235 (75.74%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGDPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLWPS 60
           MAQH+F  CLLS LL VNG PFDFFQLV+QWGPN C +  G++C+AQPQPMFTIHGLWPS
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNIC-SPAGSKCYAQPQPMFTIHGLWPS 60

Query: 61  NFSNARLSCVVHRKFNPQ-MVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPYT 120
           NFS   + C   +K  PQ  VT LEQ LN YWP+VI G NS+FW +EWRKH  C +PP+T
Sbjct: 61  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFT 120

Query: 121 IRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNN 180
           I  YF I+L  R NK YDLL ILN AGL+ Q  K+L+SQ++L+PIKNA G  PGI CN N
Sbjct: 121 IFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNIN 180

Query: 181 GITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPS--TFVWLLPQQSSIGVPDYP 233
           GIT KLQL EIVLCF  +  TLIDCP FVSN+C S   FVWLLPQQSS+GVPDYP
Sbjct: 181 GITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of PI0022781 vs. NCBI nr
Match: XP_038906187.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 6.9e-63
Identity = 122/230 (53.04%), Postives = 148/230 (64.35%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNG--DPFDFFQLVEQWGPNTCNNTLGTQCHAQPQPMFTIHGLW 60
           MA+ L    + SL L+V      +D+FQ+V+QW P TC+  +G +C AQP  MFTIHGLW
Sbjct: 1   MAKALLVISIFSLALLVFSVKAQYDYFQMVQQWPPATCSG-VGVRCRAQPPSMFTIHGLW 60

Query: 61  PSNFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPY 120
           PSN+S A L C     F+P  +  +   LNTYWP+VIRGNN  FW +EW  HG C++PP+
Sbjct: 61  PSNYSTAHLVC-TGLPFDPTQIAAIRPQLNTYWPDVIRGNNQRFWQHEWDAHGTCSDPPF 120

Query: 121 TIRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNN 180
             RQYFQ TLN+R N NYDLLAILN AGL P        Q I N I+ A GK PG+ CN 
Sbjct: 121 NQRQYFQTTLNIRMNHNYDLLAILNAAGLGPTATMVRQYQAIENAIQAATGKIPGLRCNT 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVWLLPQQSSIGV 229
           N  TG  QL+EI+LCFDKN  TLIDC  F   +CP  FVWL  Q  S+GV
Sbjct: 181 NAQTGSRQLFEIILCFDKNGVTLIDCTPFAGITCPLQFVWLHRQPWSLGV 228

[Close]

BLAST of PI0022781 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 242.3 bits (617), Expect = 4.2e-60
Identity = 120/220 (54.55%), Postives = 144/220 (65.45%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGD-PFDFFQLVEQWGPNTCN-NTLGTQCHAQPQPMFTIHGLW 60
           M Q +     LSL++ VNG  PF+FFQ VEQWGPN CN     + CH  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPY 120
           PSN SNA L C    K     V  LE  LN  WP++  GNN  FWG+EW  HG C++PP+
Sbjct: 61  PSNHSNAHLLC--QSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPF 120

Query: 121 TIRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNN 180
           ++ QYFQI+LNL   + +DLL IL  AGLNPQ  +NL  Q+I+ PI+    K PGI CN 
Sbjct: 121 SLFQYFQISLNLL--RKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVW 219
           N  TGK QL EIVLC +K+ ATLIDCP FVSN+C  +FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0022781 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 242.3 bits (617), Expect = 4.2e-60
Identity = 120/220 (54.55%), Postives = 144/220 (65.45%), Query Frame = 0


Query: 1   MAQHLFTFCLLSLLLVVNGD-PFDFFQLVEQWGPNTCN-NTLGTQCHAQPQPMFTIHGLW 60
           M Q +     LSL++ VNG  PF+FFQ VEQWGPN CN     + CH  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLSCVVHRKFNPQMVTVLEQDLNTYWPNVIRGNNSDFWGYEWRKHGACTNPPY 120
           PSN SNA L C    K     V  LE  LN  WP++  GNN  FWG+EW  HG C++PP+
Sbjct: 61  PSNHSNAHLLC--QSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPF 120

Query: 121 TIRQYFQITLNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNN 180
           ++ QYFQI+LNL   + +DLL IL  AGLNPQ  +NL  Q+I+ PI+    K PGI CN 
Sbjct: 121 SLFQYFQISLNLL--RKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNPATLIDCPRFVSNSCPSTFVW 219
           N  TGK QL EIVLC +K+ ATLIDCP FVSN+C  +FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0022781 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-19
Identity = 66/215 (30.70%), Postives = 92/215 (42.79%), Query Frame = 0


Query: 22  FDFFQLVEQWGPNTCNNTLG--TQCHAQPQPMFTIHGLWPSNFSNAR--LSCVVHRKFNP 81
           F+FF  V  W    C++  G            F IHGLWP  F+N      C     F+ 
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWP-QFNNGTWPAFCDQTNLFDI 85

Query: 82  QMVTVLEQDLNTYWPN----VIRGNNSDFWGYEWRKHGACTNPPYTIRQYFQITLNLRKN 141
             ++ L   +   W          N ++ W +EW KHG C    +    YF+   NL+  
Sbjct: 86  SKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFR--TNLKFK 145

Query: 142 KNYDLLAILNVAGLNPQMPKNLVSQD-ILNPIKNAIGKNPGIHCNNNGITGKLQLYEIVL 201
               LL IL   G+ P       S D I N IK AIG  PGI CN + + G  QL++I +
Sbjct: 146 HKVHLLNILIQKGIKPN--DGFYSLDEIKNAIKCAIGFAPGIECNED-VKGNKQLFQIYI 205

Query: 202 CFDKNPATLIDCPRFVSNSCPSTFVW-LLPQQSSI 227
           C D      ++CP     SC S   +  LP++ S+
Sbjct: 206 CLDNYAKEFVECPYVPDKSCASKIKFPSLPERDSL 234

[Close]

BLAST of PI0022781 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-19
Identity = 64/213 (30.05%), Postives = 86/213 (40.38%), Query Frame = 0


Query: 22  FDFFQLVEQWGPNTCNNT-----LGTQCHAQPQP-MFTIHGLWPSNFSNARLSCVVHRKF 81
           FD+F L  QW    C  T         C     P  FTIHGLWP     +  SC     F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 82  NPQMVTVLEQDLNTYWPNV-------IRGNNSDFWGYEWRKHGACTNPPYTIR-QYFQIT 141
             + ++ L   L  YWP++         G    FWG+EW KHG C++P +     YF  T
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 142 LNLRKNKNYDLLAILNVAGLNPQMPKNLVSQDILNPIKNAIGKNPGIHCNNNGITGKLQL 201
           LNL    N  +  +L  AG      +      I+  I+NA    P + C  + I      
Sbjct: 152 LNLYLKHN--VTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAID----- 211

Query: 202 YEIVLCF--DKNPATLI---------DCPRFVS 210
            EI +CF  D  P   +          CP++VS
Sbjct: 212 -EIRICFYKDFKPRDCVGSQDLTSRKSCPKYVS 236

The following BLAST results are available for this feature:

BLAST of PI0022781 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	6.6e-32	34.01	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80022	3.5e-25	33.18	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
Q38717	1.0e-24	34.39	Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1	[more]
O80323	2.3e-24	35.61	Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
O80325	8.6e-24	32.02	Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of PI0022781 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DP91	2.4e-85	67.95	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00...	[more]
A0A0A0LAB0	2.6e-84	65.96	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1	[more]
A0A0A0LAN6	2.0e-60	54.55	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A6J1EXD5	1.7e-59	51.58	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1F837	3.8e-59	52.56	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]

BLAST of PI0022781 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK25090.1	4.9e-85	67.95	S-RNase [Cucumis melo var. makuwa]	[more]
XP_011651927.1	5.5e-84	65.96	ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C...	[more]
XP_038906187.1	6.9e-63	53.04	ribonuclease MC-like [Benincasa hispida]	[more]
KAE8651058.1	4.2e-60	54.55	hypothetical protein Csa_002465 [Cucumis sativus]	[more]
XP_031738690.1	4.2e-60	54.55	ribonuclease MC-like [Cucumis sativus]	[more]

BLAST of PI0022781 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14210.1	1.7e-19	30.70	Ribonuclease T2 family protein	[more]
AT2G39780.1	3.8e-19	30.05	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 22..204 e-value: 1.5E-40 score: 139.3
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 11..214
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 17..227 e-value: 9.1E-51 score: 174.8
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 22..221
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 11..214
None	No IPR available	CDD	cd00374	RNase_T2	coord: 22..219 e-value: 1.44E-56 score: 176.878
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 102..113
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 52..59

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0022781.1	PI0022781.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006401	RNA catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding