PI0022458 (gene) Melon (PI 482460) v1

BLAST of PI0022458 vs. ExPASy Swiss-Prot
Match: Q9D8Z1 (Activating signal cointegrator 1 complex subunit 1 OS=Mus musculus OX=10090 GN=Ascc1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 7.7e-17
Identity = 59/195 (30.26%), Postives = 92/195 (47.18%), Query Frame = 0

Query: 324 DLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVV-DALDNRPVLIKLKG 383
           D G+D +IF  PK  HLT+ ML L +++ +    E+L+    + + D    RP+ +++ G
Sbjct: 163 DRGVDSTIFQNPKKLHLTIGMLVLLSEQEIQQTCEILQRCKEEFINDISGGRPLEVEMAG 222

Query: 384 LDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHATVM 443
           ++ M    A   VLYA V       RL      +++ F   GL++++     +KLHATVM
Sbjct: 223 IEYMNDDPAMVDVLYAKVHMKDGSNRLQELVDRVLERFQSLGLIVKE--WTSVKLHATVM 282

Query: 444 NARHRKSKKK---------------KKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFV 503
           N   RK                   K+ +SFD R I K + +  +G   +   H+SQRF 
Sbjct: 283 NTLLRKDPNAEGRYNLYTADGKYIFKERESFDGRNILKTFENFYFGSLRLNSIHISQRFT 342

BLAST of PI0022458 vs. ExPASy Swiss-Prot
Match: Q8N9N2 (Activating signal cointegrator 1 complex subunit 1 OS=Homo sapiens OX=9606 GN=ASCC1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.4e-10
Identity = 48/160 (30.00%), Postives = 76/160 (47.50%), Query Frame = 0

Query: 324 DLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVV-DALDNRPVLIKLKG 383
           D G+D SIF  PK  HLT+ ML L ++E +    E+L+    + + D    +P+ +++ G
Sbjct: 192 DHGVDSSIFQNPKKLHLTIGMLVLLSEEEIQQTCEMLQQCKEEFINDISGGKPLEVEMAG 251

Query: 384 LDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHATVM 443
           ++ M        VLYA V       RL      +++ F  +GL++++     +KLHATVM
Sbjct: 252 IEYMNDDPGMVDVLYAKVHMKDGSNRLQELVDRVLERFQASGLIVKE--WNSVKLHATVM 311

Query: 444 NARHRKSKKK---------------KKFDSFDAREIFKQY 468
           N   RK                   K+ +SFD R I K +
Sbjct: 312 NTLFRKDPNAEGRYNLYTAEGKYIFKERESFDGRNILKSF 349

BLAST of PI0022458 vs. ExPASy TrEMBL
Match: A0A1S3CGC2 (activating signal cointegrator 1 complex subunit 1 OS=Cucumis melo OX=3656 GN=LOC103500600 PE=4 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 9.8e-257
Identity = 464/493 (94.12%), Postives = 477/493 (96.75%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYTT YVLHQTGLYAYNGWRLNANM+GKTEF+SAADQKKKRKTISQAWRPVCTHA
Sbjct: 22  VDRFLKYTTPYVLHQTGLYAYNGWRLNANMTGKTEFKSAADQKKKRKTISQAWRPVCTHA 81

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGGDTN 139
           C SEDLSVKDDRVESEDGSQVQEM CRMH+ST SAQ  VEV EEINVVTELSVN+GGDTN
Sbjct: 82  CSSEDLSVKDDRVESEDGSQVQEMDCRMHSSTASAQ-PVEVVEEINVVTELSVNVGGDTN 141

Query: 140 LEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG 199
           LEGQSVPSGEKFSVKL+VGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSK+EEFVVIEG
Sbjct: 142 LEGQSVPSGEKFSVKLNVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKREEFVVIEG 201

Query: 200 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 259
           NSVDSVTKASEKIQSIIDEA KSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC
Sbjct: 202 NSVDSVTKASEKIQSIIDEATKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 261

Query: 260 LDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKV 319
           LDE EDSDTNEDNTDNE+EVQHTV APDVAVELQVDDKREQIK NINIPVVSY PKTSKV
Sbjct: 262 LDETEDSDTNEDNTDNEVEVQHTVNAPDVAVELQVDDKREQIKANINIPVVSYLPKTSKV 321

Query: 320 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIK 379
           STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++DALDNRPVLI+
Sbjct: 322 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIR 381

Query: 380 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHA 439
           LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQV+IDAFTEAGLVLEKDAK KLKLHA
Sbjct: 382 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVVIDAFTEAGLVLEKDAKHKLKLHA 441

Query: 440 TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS 499
           TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS
Sbjct: 442 TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS 501

Query: 500 IPFP-DEQHMQVD 512
           IPFP ++QHMQVD
Sbjct: 502 IPFPHEQQHMQVD 513

BLAST of PI0022458 vs. ExPASy TrEMBL
Match: A0A0A0K963 (KH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447090 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.0e-254
Identity = 461/492 (93.70%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYT  +VLHQTGLYAYNGWRLNANM+GKTEFRSAADQKKKRKTISQAWRPVCTHA
Sbjct: 68  VDRFLKYTAPFVLHQTGLYAYNGWRLNANMTGKTEFRSAADQKKKRKTISQAWRPVCTHA 127

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGGDTN 139
           CPSEDLSV+DDRVESEDGSQVQEM  RMHTST SAQ  VEVAEEINVVTELSVNMGGDTN
Sbjct: 128 CPSEDLSVEDDRVESEDGSQVQEMDVRMHTST-SAQ-PVEVAEEINVVTELSVNMGGDTN 187

Query: 140 LEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG 199
           LEGQSV SGEKFSVKLDVGSSLIRFVRGKGGSTQERIE+EMGVKIMIPSSK+EEFVVIEG
Sbjct: 188 LEGQSVTSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEKEMGVKIMIPSSKREEFVVIEG 247

Query: 200 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 259
           NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC
Sbjct: 248 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 307

Query: 260 LDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKV 319
           LD+AEDSDTNED+TDNE+EVQHTV APDVAVELQVD+KREQIKVNINIP+VSY PKTSKV
Sbjct: 308 LDDAEDSDTNEDHTDNEVEVQHTVNAPDVAVELQVDNKREQIKVNINIPIVSYLPKTSKV 367

Query: 320 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIK 379
           STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++D LDNRPVLI+
Sbjct: 368 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDTLDNRPVLIR 427

Query: 380 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHA 439
           LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQ+II+AFTEAGLVLEKDAKQKLKLHA
Sbjct: 428 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQLIINAFTEAGLVLEKDAKQKLKLHA 487

Query: 440 TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS 499
           TVMNARHRKSKKKKKFDSFDAREIFK+YGSEEWGEYHIREAHLSQRF FDENGYYHCCAS
Sbjct: 488 TVMNARHRKSKKKKKFDSFDAREIFKEYGSEEWGEYHIREAHLSQRFAFDENGYYHCCAS 547

Query: 500 IPFPDEQHMQVD 512
           IPFP EQHMQVD
Sbjct: 548 IPFPHEQHMQVD 557

BLAST of PI0022458 vs. ExPASy TrEMBL
Match: A0A5D3C0W5 (Activating signal cointegrator 1 complex subunit 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002070 PE=4 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.4e-247
Identity = 455/506 (89.92%), Postives = 469/506 (92.69%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYTT YVLHQTGLYAYNGWRLNANM+GKTEF+SAADQKKKRKTISQAWRPVCTHA
Sbjct: 46  VDRFLKYTTPYVLHQTGLYAYNGWRLNANMTGKTEFKSAADQKKKRKTISQAWRPVCTHA 105

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGGDTN 139
           C SEDLSVKDDRVESEDGSQVQEM CRMH+ST SAQ  VEV EEINVVTELSVN+GGDTN
Sbjct: 106 CSSEDLSVKDDRVESEDGSQVQEMDCRMHSSTASAQ-PVEVVEEINVVTELSVNVGGDTN 165

Query: 140 LEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG 199
           LEGQSVPSGEKFSVKL+VGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSK+EEFVVIEG
Sbjct: 166 LEGQSVPSGEKFSVKLNVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKREEFVVIEG 225

Query: 200 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 259
           NSVDSVTKASEKIQSIIDEA KSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC
Sbjct: 226 NSVDSVTKASEKIQSIIDEATKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 285

Query: 260 LDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKV 319
           LDE EDSDTNEDNTDNE+EVQHTV APDVAVELQVDDKREQIK NINIPVVSY PKTSKV
Sbjct: 286 LDETEDSDTNEDNTDNEVEVQHTVNAPDVAVELQVDDKREQIKANINIPVVSYLPKTSKV 345

Query: 320 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIK 379
           STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++DALDNRPVLI+
Sbjct: 346 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIR 405

Query: 380 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLK--L 439
           LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQV+IDAFTEAGLVLEKDAK KLK  L
Sbjct: 406 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVVIDAFTEAGLVLEKDAKHKLKFLL 465

Query: 440 HATVM------------NARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQR 499
            A +              ++   SKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQR
Sbjct: 466 FANLYVYNPELFDFMAPQSQSLSSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQR 525

Query: 500 FVFDENGYYHCCASIPFPDEQHMQVD 512
           FVFDENGYYHCCASIPFP EQHMQVD
Sbjct: 526 FVFDENGYYHCCASIPFPHEQHMQVD 550

BLAST of PI0022458 vs. ExPASy TrEMBL
Match: A0A6J1GQ24 (uncharacterized protein LOC111456441 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456441 PE=4 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.9e-236
Identity = 438/499 (87.78%), Postives = 458/499 (91.78%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYT+ +V  Q GLY YNGW LNANM+GK EFR  ADQKKKRKTI+QAWRPVCT A
Sbjct: 10  VERFLKYTSPFVPQQPGLYTYNGWSLNANMAGKKEFRRVADQKKKRKTITQAWRPVCTQA 69

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSV------N 139
            PSEDL VK+DRVESEDGS+VQE    +HTSTVSAQ+ VEVAEEINVVT+LSV      N
Sbjct: 70  SPSEDLVVKNDRVESEDGSRVQE----IHTSTVSAQNVVEVAEEINVVTDLSVGSSAFPN 129

Query: 140 MGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEE 199
            GGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSKKEE
Sbjct: 130 EGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEE 189

Query: 200 FVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSIL 259
           FVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSIL
Sbjct: 190 FVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSIL 249

Query: 260 RSSESCLDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYP 319
            SSESCLDEAEDSDTNEDNTDNE+EV  TVK PDVAVEL+VDDK E +KVNINIPVVSYP
Sbjct: 250 GSSESCLDEAEDSDTNEDNTDNEVEVHDTVKEPDVAVELKVDDKEEHVKVNINIPVVSYP 309

Query: 320 PKTSKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDN 379
           PK SK STPSDLGIDKS+FIKPKTFHLTVLMLKLWNKERVDAASEVLRGIS+K++DALDN
Sbjct: 310 PKASKTSTPSDLGIDKSVFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISTKIMDALDN 369

Query: 380 RPVLIKLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQ 439
           RPVLIKLKGLDCMRGSLAKARVLYAPVEEI DEGRLLRACQVIIDAF EAGLVLEKDAKQ
Sbjct: 370 RPVLIKLKGLDCMRGSLAKARVLYAPVEEIDDEGRLLRACQVIIDAFDEAGLVLEKDAKQ 429

Query: 440 KLKLHATVMNARHRK-SKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENG 499
           KLKLHATVMNARHRK +K+KKKFDSFD REIFKQYGSEEWG YHIREAHLSQRF FDENG
Sbjct: 430 KLKLHATVMNARHRKRNKRKKKFDSFDTREIFKQYGSEEWGVYHIREAHLSQRFAFDENG 489

Query: 500 YYHCCASIPFPDEQHMQVD 512
           YYHCCASIPFPDEQ MQV+
Sbjct: 490 YYHCCASIPFPDEQ-MQVE 503

BLAST of PI0022458 vs. ExPASy TrEMBL
Match: A0A6J1GRE7 (uncharacterized protein LOC111456441 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456441 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.9e-232
Identity = 430/483 (89.03%), Postives = 448/483 (92.75%), Query Frame = 0

Query: 36  GLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHACPSEDLSVKDDRVESE 95
           GLY YNGW LNANM+GK EFR  ADQKKKRKTI+QAWRPVCT A PSEDL VK+DRVESE
Sbjct: 23  GLYTYNGWSLNANMAGKKEFRRVADQKKKRKTITQAWRPVCTQASPSEDLVVKNDRVESE 82

Query: 96  DGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSV------NMGGDTNLEGQSVPSGE 155
           DGS+VQE    +HTSTVSAQ+ VEVAEEINVVT+LSV      N GGDTNLEGQSVPSGE
Sbjct: 83  DGSRVQE----IHTSTVSAQNVVEVAEEINVVTDLSVGSSAFPNEGGDTNLEGQSVPSGE 142

Query: 156 KFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEGNSVDSVTKAS 215
           KFSVKLDVGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSKKEEFVVIEGNSVDSVTKAS
Sbjct: 143 KFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVVIEGNSVDSVTKAS 202

Query: 216 EKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESCLDEAEDSDTN 275
           EKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSIL SSESCLDEAEDSDTN
Sbjct: 203 EKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILGSSESCLDEAEDSDTN 262

Query: 276 EDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKVSTPSDLGIDK 335
           EDNTDNE+EV  TVK PDVAVEL+VDDK E +KVNINIPVVSYPPK SK STPSDLGIDK
Sbjct: 263 EDNTDNEVEVHDTVKEPDVAVELKVDDKEEHVKVNINIPVVSYPPKASKTSTPSDLGIDK 322

Query: 336 SIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIKLKGLDCMRGS 395
           S+FIKPKTFHLTVLMLKLWNKERVDAASEVLRGIS+K++DALDNRPVLIKLKGLDCMRGS
Sbjct: 323 SVFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISTKIMDALDNRPVLIKLKGLDCMRGS 382

Query: 396 LAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHATVMNARHRK- 455
           LAKARVLYAPVEEI DEGRLLRACQVIIDAF EAGLVLEKDAKQKLKLHATVMNARHRK 
Sbjct: 383 LAKARVLYAPVEEIDDEGRLLRACQVIIDAFDEAGLVLEKDAKQKLKLHATVMNARHRKR 442

Query: 456 SKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCASIPFPDEQHM 512
           +K+KKKFDSFD REIFKQYGSEEWG YHIREAHLSQRF FDENGYYHCCASIPFPDEQ M
Sbjct: 443 NKRKKKFDSFDTREIFKQYGSEEWGVYHIREAHLSQRFAFDENGYYHCCASIPFPDEQ-M 500

BLAST of PI0022458 vs. NCBI nr
Match: XP_008462176.1 (PREDICTED: activating signal cointegrator 1 complex subunit 1 [Cucumis melo])

HSP 1 Score: 895.6 bits (2313), Expect = 2.0e-256
Identity = 464/493 (94.12%), Postives = 477/493 (96.75%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYTT YVLHQTGLYAYNGWRLNANM+GKTEF+SAADQKKKRKTISQAWRPVCTHA
Sbjct: 22  VDRFLKYTTPYVLHQTGLYAYNGWRLNANMTGKTEFKSAADQKKKRKTISQAWRPVCTHA 81

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGGDTN 139
           C SEDLSVKDDRVESEDGSQVQEM CRMH+ST SAQ  VEV EEINVVTELSVN+GGDTN
Sbjct: 82  CSSEDLSVKDDRVESEDGSQVQEMDCRMHSSTASAQ-PVEVVEEINVVTELSVNVGGDTN 141

Query: 140 LEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG 199
           LEGQSVPSGEKFSVKL+VGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSK+EEFVVIEG
Sbjct: 142 LEGQSVPSGEKFSVKLNVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKREEFVVIEG 201

Query: 200 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 259
           NSVDSVTKASEKIQSIIDEA KSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC
Sbjct: 202 NSVDSVTKASEKIQSIIDEATKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 261

Query: 260 LDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKV 319
           LDE EDSDTNEDNTDNE+EVQHTV APDVAVELQVDDKREQIK NINIPVVSY PKTSKV
Sbjct: 262 LDETEDSDTNEDNTDNEVEVQHTVNAPDVAVELQVDDKREQIKANINIPVVSYLPKTSKV 321

Query: 320 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIK 379
           STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++DALDNRPVLI+
Sbjct: 322 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIR 381

Query: 380 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHA 439
           LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQV+IDAFTEAGLVLEKDAK KLKLHA
Sbjct: 382 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVVIDAFTEAGLVLEKDAKHKLKLHA 441

Query: 440 TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS 499
           TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS
Sbjct: 442 TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS 501

Query: 500 IPFP-DEQHMQVD 512
           IPFP ++QHMQVD
Sbjct: 502 IPFPHEQQHMQVD 513

BLAST of PI0022458 vs. NCBI nr
Match: XP_004141755.1 (activating signal cointegrator 1 complex subunit 1 isoform X2 [Cucumis sativus] >KAE8646472.1 hypothetical protein Csa_016081 [Cucumis sativus])

HSP 1 Score: 887.9 bits (2293), Expect = 4.2e-254
Identity = 461/492 (93.70%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYT  +VLHQTGLYAYNGWRLNANM+GKTEFRSAADQKKKRKTISQAWRPVCTHA
Sbjct: 10  VDRFLKYTAPFVLHQTGLYAYNGWRLNANMTGKTEFRSAADQKKKRKTISQAWRPVCTHA 69

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGGDTN 139
           CPSEDLSV+DDRVESEDGSQVQEM  RMHTST SAQ  VEVAEEINVVTELSVNMGGDTN
Sbjct: 70  CPSEDLSVEDDRVESEDGSQVQEMDVRMHTST-SAQ-PVEVAEEINVVTELSVNMGGDTN 129

Query: 140 LEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG 199
           LEGQSV SGEKFSVKLDVGSSLIRFVRGKGGSTQERIE+EMGVKIMIPSSK+EEFVVIEG
Sbjct: 130 LEGQSVTSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEKEMGVKIMIPSSKREEFVVIEG 189

Query: 200 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 259
           NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC
Sbjct: 190 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 249

Query: 260 LDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKV 319
           LD+AEDSDTNED+TDNE+EVQHTV APDVAVELQVD+KREQIKVNINIP+VSY PKTSKV
Sbjct: 250 LDDAEDSDTNEDHTDNEVEVQHTVNAPDVAVELQVDNKREQIKVNINIPIVSYLPKTSKV 309

Query: 320 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIK 379
           STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++D LDNRPVLI+
Sbjct: 310 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDTLDNRPVLIR 369

Query: 380 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHA 439
           LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQ+II+AFTEAGLVLEKDAKQKLKLHA
Sbjct: 370 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQLIINAFTEAGLVLEKDAKQKLKLHA 429

Query: 440 TVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHCCAS 499
           TVMNARHRKSKKKKKFDSFDAREIFK+YGSEEWGEYHIREAHLSQRF FDENGYYHCCAS
Sbjct: 430 TVMNARHRKSKKKKKFDSFDAREIFKEYGSEEWGEYHIREAHLSQRFAFDENGYYHCCAS 489

Query: 500 IPFPDEQHMQVD 512
           IPFP EQHMQVD
Sbjct: 490 IPFPHEQHMQVD 499

BLAST of PI0022458 vs. NCBI nr
Match: XP_031745174.1 (activating signal cointegrator 1 complex subunit 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 882.5 bits (2279), Expect = 1.8e-252
Identity = 461/495 (93.13%), Postives = 477/495 (96.36%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYT  +VLHQTGLYAYNGWRLNANM+GKTEFRSAADQKKKRKTISQAWRPVCTHA
Sbjct: 10  VDRFLKYTAPFVLHQTGLYAYNGWRLNANMTGKTEFRSAADQKKKRKTISQAWRPVCTHA 69

Query: 80  CPSE---DLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGG 139
           CPSE   DLSV+DDRVESEDGSQVQEM  RMHTST SAQ  VEVAEEINVVTELSVNMGG
Sbjct: 70  CPSEGRLDLSVEDDRVESEDGSQVQEMDVRMHTST-SAQ-PVEVAEEINVVTELSVNMGG 129

Query: 140 DTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVV 199
           DTNLEGQSV SGEKFSVKLDVGSSLIRFVRGKGGSTQERIE+EMGVKIMIPSSK+EEFVV
Sbjct: 130 DTNLEGQSVTSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEKEMGVKIMIPSSKREEFVV 189

Query: 200 IEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSS 259
           IEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSS
Sbjct: 190 IEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSS 249

Query: 260 ESCLDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKT 319
           ESCLD+AEDSDTNED+TDNE+EVQHTV APDVAVELQVD+KREQIKVNINIP+VSY PKT
Sbjct: 250 ESCLDDAEDSDTNEDHTDNEVEVQHTVNAPDVAVELQVDNKREQIKVNINIPIVSYLPKT 309

Query: 320 SKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPV 379
           SKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++D LDNRPV
Sbjct: 310 SKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDTLDNRPV 369

Query: 380 LIKLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLK 439
           LI+LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQ+II+AFTEAGLVLEKDAKQKLK
Sbjct: 370 LIRLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQLIINAFTEAGLVLEKDAKQKLK 429

Query: 440 LHATVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGYYHC 499
           LHATVMNARHRKSKKKKKFDSFDAREIFK+YGSEEWGEYHIREAHLSQRF FDENGYYHC
Sbjct: 430 LHATVMNARHRKSKKKKKFDSFDAREIFKEYGSEEWGEYHIREAHLSQRFAFDENGYYHC 489

Query: 500 CASIPFPDEQHMQVD 512
           CASIPFP EQHMQVD
Sbjct: 490 CASIPFPHEQHMQVD 502

BLAST of PI0022458 vs. NCBI nr
Match: KAA0059318.1 (activating signal cointegrator 1 complex subunit 1 [Cucumis melo var. makuwa] >TYK04009.1 activating signal cointegrator 1 complex subunit 1 [Cucumis melo var. makuwa])

HSP 1 Score: 864.4 bits (2232), Expect = 5.0e-247
Identity = 455/506 (89.92%), Postives = 469/506 (92.69%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYTT YVLHQTGLYAYNGWRLNANM+GKTEF+SAADQKKKRKTISQAWRPVCTHA
Sbjct: 46  VDRFLKYTTPYVLHQTGLYAYNGWRLNANMTGKTEFKSAADQKKKRKTISQAWRPVCTHA 105

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSVNMGGDTN 139
           C SEDLSVKDDRVESEDGSQVQEM CRMH+ST SAQ  VEV EEINVVTELSVN+GGDTN
Sbjct: 106 CSSEDLSVKDDRVESEDGSQVQEMDCRMHSSTASAQ-PVEVVEEINVVTELSVNVGGDTN 165

Query: 140 LEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG 199
           LEGQSVPSGEKFSVKL+VGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSK+EEFVVIEG
Sbjct: 166 LEGQSVPSGEKFSVKLNVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKREEFVVIEG 225

Query: 200 NSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 259
           NSVDSVTKASEKIQSIIDEA KSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC
Sbjct: 226 NSVDSVTKASEKIQSIIDEATKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESC 285

Query: 260 LDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYPPKTSKV 319
           LDE EDSDTNEDNTDNE+EVQHTV APDVAVELQVDDKREQIK NINIPVVSY PKTSKV
Sbjct: 286 LDETEDSDTNEDNTDNEVEVQHTVNAPDVAVELQVDDKREQIKANINIPVVSYLPKTSKV 345

Query: 320 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDNRPVLIK 379
           STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK++DALDNRPVLI+
Sbjct: 346 STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIR 405

Query: 380 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQKLK--L 439
           LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQV+IDAFTEAGLVLEKDAK KLK  L
Sbjct: 406 LKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVVIDAFTEAGLVLEKDAKHKLKFLL 465

Query: 440 HATVM------------NARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQR 499
            A +              ++   SKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQR
Sbjct: 466 FANLYVYNPELFDFMAPQSQSLSSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQR 525

Query: 500 FVFDENGYYHCCASIPFPDEQHMQVD 512
           FVFDENGYYHCCASIPFP EQHMQVD
Sbjct: 526 FVFDENGYYHCCASIPFPHEQHMQVD 550

BLAST of PI0022458 vs. NCBI nr
Match: XP_038899990.1 (uncharacterized protein LOC120087160 isoform X1 [Benincasa hispida])

HSP 1 Score: 862.4 bits (2227), Expect = 1.9e-246
Identity = 451/498 (90.56%), Postives = 467/498 (93.78%), Query Frame = 0

Query: 20  VHRFSKYTTTYVLHQTGLYAYNGWRLNANMSGKTEFRSAADQKKKRKTISQAWRPVCTHA 79
           V RF KYT+ YVLHQ GLYAYNGW LNANM+ K EFRSAADQKKKRKTISQAW+PVCT A
Sbjct: 23  VDRFLKYTSPYVLHQPGLYAYNGWSLNANMTNKKEFRSAADQKKKRKTISQAWKPVCTQA 82

Query: 80  CPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVEVAEEINVVTELSV------N 139
            PSEDLSVKDDRVE EDGS+VQEM CRMHTST SA++ VEVAEEINVVTELSV      N
Sbjct: 83  SPSEDLSVKDDRVELEDGSRVQEMDCRMHTSTASARNVVEVAEEINVVTELSVESSAFPN 142

Query: 140 MGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEE 199
           M GD NLEGQSVPS EKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEE
Sbjct: 143 MDGDANLEGQSVPSDEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEE 202

Query: 200 FVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSIL 259
           FVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLA+HPELVEKL NFQNSIL
Sbjct: 203 FVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLALHPELVEKLTNFQNSIL 262

Query: 260 RSSESCLDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKREQIKVNINIPVVSYP 319
           RSSESCLDEAEDSDTNEDNTDNE+EVQHTV+APDVAVEL+VDDKREQIKVNINIPVVSYP
Sbjct: 263 RSSESCLDEAEDSDTNEDNTDNEVEVQHTVEAPDVAVELKVDDKREQIKVNINIPVVSYP 322

Query: 320 PKTSKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKVVDALDN 379
            KTSK STPSDLGIDKSIFIKP TFHLTVLMLKLWNK+RVDAASEVLRGISSK++DALDN
Sbjct: 323 SKTSKASTPSDLGIDKSIFIKPNTFHLTVLMLKLWNKDRVDAASEVLRGISSKIMDALDN 382

Query: 380 RPVLIKLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVIIDAFTEAGLVLEKDAKQ 439
           R VLI+LKGLDCMRGSLAKARVLYAPVEEIG+EGRLLRACQVII+AFTEAGLVLEKDAKQ
Sbjct: 383 RQVLIRLKGLDCMRGSLAKARVLYAPVEEIGNEGRLLRACQVIINAFTEAGLVLEKDAKQ 442

Query: 440 KLKLHATVMNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDENGY 499
           KLKLHATVMNARHRKSKKKKKFDSFDAREIFK YGSEEWGEYHIRE HLSQRF FDENGY
Sbjct: 443 KLKLHATVMNARHRKSKKKKKFDSFDAREIFKHYGSEEWGEYHIREVHLSQRFSFDENGY 502

Query: 500 YHCCASIPFPDEQHMQVD 512
           YHCCASIPFPDE HMQVD
Sbjct: 503 YHCCASIPFPDE-HMQVD 519

BLAST of PI0022458 vs. TAIR 10
Match: AT3G16230.2 (Predicted eukaryotic LigT )

HSP 1 Score: 466.5 bits (1199), Expect = 2.8e-131
Identity = 267/455 (58.68%), Postives = 326/455 (71.65%), Query Frame = 0

Query: 56  RSAADQKKKRKTISQAWRPVCTHACPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQ 115
           +SA D  KK+K ++  WRP+          S +   V +E G++VQE+     +S VS  
Sbjct: 38  KSAMDGYKKQKMVNLVWRPI----------STQTSSVIAEAGNEVQEVAQCSKSSDVSK- 97

Query: 116 HAVEVAEEINVVTELSVNMGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQER 175
              EV E             G+T     SV S  K SV L+VG+SLI+F+RGK G+TQ +
Sbjct: 98  ---EVIE-------------GET----ASVVSAGKHSVSLEVGASLIKFIRGKEGTTQMK 157

Query: 176 IEEEMGVKIMIPSSKKEEFVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPL 235
           +EEEMGVKI++PSS+ ++ + IEG SVD VTKAS++I +IIDE ++SPSLDYSHFVSLPL
Sbjct: 158 LEEEMGVKIILPSSRNKDHISIEGGSVDCVTKASKRIATIIDEVVRSPSLDYSHFVSLPL 217

Query: 236 AIHPELVEKLINFQNSILRSSESCLDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVD 295
           AIHPELV+KL+NFQNSIL    S   + +D   N   T              VAV+L+ +
Sbjct: 218 AIHPELVDKLVNFQNSIL-GIHSIASDKQDDQANRATT-------------SVAVDLKAN 277

Query: 296 DKREQIKVNI-NIPVVSYPPKT-SKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERV 355
            +  Q+ V I +IP+VSYPPK  SK ST  DLGI+KSIFIKP TFHLTV+MLKLWNK+RV
Sbjct: 278 SETNQVNVGIKSIPIVSYPPKAKSKSSTLLDLGIEKSIFIKPSTFHLTVVMLKLWNKDRV 337

Query: 356 DAASEVLRGISSKVVDALDNRPVLIKLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRAC 415
           +AA +VL+ I   V+DALDN+PV I+LKGLDCMRG L K RVLYAPVEEIGDEGRLLRAC
Sbjct: 338 NAACDVLKSIFPSVMDALDNKPVFIRLKGLDCMRGPLDKTRVLYAPVEEIGDEGRLLRAC 397

Query: 416 QVIIDAFTEAGLVLEKDAKQKLKLHATVMNARHRKSKK--KKKFDSFDAREIFKQYGSEE 475
           QVI DAF +AGLVLEKDAKQ LKLH TVMNARHRK +K  KKK ++FDAREI KQ+G+E+
Sbjct: 398 QVITDAFVKAGLVLEKDAKQSLKLHVTVMNARHRKRRKNNKKKMETFDAREIHKQFGNED 447

Query: 476 WGEYHIREAHLSQRFVFDENGYYHCCASIPFPDEQ 507
           WGEY I+EAHLSQRFVFD+NGYY CC SIPFP EQ
Sbjct: 458 WGEYLIQEAHLSQRFVFDQNGYYRCCGSIPFPGEQ 447

BLAST of PI0022458 vs. TAIR 10
Match: AT3G16230.1 (Predicted eukaryotic LigT )

HSP 1 Score: 463.4 bits (1191), Expect = 2.4e-130
Identity = 265/451 (58.76%), Postives = 323/451 (71.62%), Query Frame = 0

Query: 60  DQKKKRKTISQAWRPVCTHACPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVE 119
           D  KK+K ++  WRP+          S +   V +E G++VQE+     +S VS     E
Sbjct: 2   DGYKKQKMVNLVWRPI----------STQTSSVIAEAGNEVQEVAQCSKSSDVSK----E 61

Query: 120 VAEEINVVTELSVNMGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEE 179
           V E             G+T     SV S  K SV L+VG+SLI+F+RGK G+TQ ++EEE
Sbjct: 62  VIE-------------GET----ASVVSAGKHSVSLEVGASLIKFIRGKEGTTQMKLEEE 121

Query: 180 MGVKIMIPSSKKEEFVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHP 239
           MGVKI++PSS+ ++ + IEG SVD VTKAS++I +IIDE ++SPSLDYSHFVSLPLAIHP
Sbjct: 122 MGVKIILPSSRNKDHISIEGGSVDCVTKASKRIATIIDEVVRSPSLDYSHFVSLPLAIHP 181

Query: 240 ELVEKLINFQNSILRSSESCLDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKRE 299
           ELV+KL+NFQNSIL    S   + +D   N   T              VAV+L+ + +  
Sbjct: 182 ELVDKLVNFQNSIL-GIHSIASDKQDDQANRATT-------------SVAVDLKANSETN 241

Query: 300 QIKVNI-NIPVVSYPPKT-SKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAAS 359
           Q+ V I +IP+VSYPPK  SK ST  DLGI+KSIFIKP TFHLTV+MLKLWNK+RV+AA 
Sbjct: 242 QVNVGIKSIPIVSYPPKAKSKSSTLLDLGIEKSIFIKPSTFHLTVVMLKLWNKDRVNAAC 301

Query: 360 EVLRGISSKVVDALDNRPVLIKLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVII 419
           +VL+ I   V+DALDN+PV I+LKGLDCMRG L K RVLYAPVEEIGDEGRLLRACQVI 
Sbjct: 302 DVLKSIFPSVMDALDNKPVFIRLKGLDCMRGPLDKTRVLYAPVEEIGDEGRLLRACQVIT 361

Query: 420 DAFTEAGLVLEKDAKQKLKLHATVMNARHRKSKK--KKKFDSFDAREIFKQYGSEEWGEY 479
           DAF +AGLVLEKDAKQ LKLH TVMNARHRK +K  KKK ++FDAREI KQ+G+E+WGEY
Sbjct: 362 DAFVKAGLVLEKDAKQSLKLHVTVMNARHRKRRKNNKKKMETFDAREIHKQFGNEDWGEY 407

Query: 480 HIREAHLSQRFVFDENGYYHCCASIPFPDEQ 507
            I+EAHLSQRFVFD+NGYY CC SIPFP EQ
Sbjct: 422 LIQEAHLSQRFVFDQNGYYRCCGSIPFPGEQ 407

BLAST of PI0022458 vs. TAIR 10
Match: AT3G16230.3 (Predicted eukaryotic LigT )

HSP 1 Score: 463.4 bits (1191), Expect = 2.4e-130
Identity = 265/451 (58.76%), Postives = 323/451 (71.62%), Query Frame = 0

Query: 60  DQKKKRKTISQAWRPVCTHACPSEDLSVKDDRVESEDGSQVQEMGCRMHTSTVSAQHAVE 119
           D  KK+K ++  WRP+          S +   V +E G++VQE+     +S VS     E
Sbjct: 2   DGYKKQKMVNLVWRPI----------STQTSSVIAEAGNEVQEVAQCSKSSDVSK----E 61

Query: 120 VAEEINVVTELSVNMGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQERIEEE 179
           V E             G+T     SV S  K SV L+VG+SLI+F+RGK G+TQ ++EEE
Sbjct: 62  VIE-------------GET----ASVVSAGKHSVSLEVGASLIKFIRGKEGTTQMKLEEE 121

Query: 180 MGVKIMIPSSKKEEFVVIEGNSVDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHP 239
           MGVKI++PSS+ ++ + IEG SVD VTKAS++I +IIDE ++SPSLDYSHFVSLPLAIHP
Sbjct: 122 MGVKIILPSSRNKDHISIEGGSVDCVTKASKRIATIIDEVVRSPSLDYSHFVSLPLAIHP 181

Query: 240 ELVEKLINFQNSILRSSESCLDEAEDSDTNEDNTDNELEVQHTVKAPDVAVELQVDDKRE 299
           ELV+KL+NFQNSIL    S   + +D   N   T              VAV+L+ + +  
Sbjct: 182 ELVDKLVNFQNSIL-GIHSIASDKQDDQANRATT-------------SVAVDLKANSETN 241

Query: 300 QIKVNI-NIPVVSYPPKT-SKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAAS 359
           Q+ V I +IP+VSYPPK  SK ST  DLGI+KSIFIKP TFHLTV+MLKLWNK+RV+AA 
Sbjct: 242 QVNVGIKSIPIVSYPPKAKSKSSTLLDLGIEKSIFIKPSTFHLTVVMLKLWNKDRVNAAC 301

Query: 360 EVLRGISSKVVDALDNRPVLIKLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVII 419
           +VL+ I   V+DALDN+PV I+LKGLDCMRG L K RVLYAPVEEIGDEGRLLRACQVI 
Sbjct: 302 DVLKSIFPSVMDALDNKPVFIRLKGLDCMRGPLDKTRVLYAPVEEIGDEGRLLRACQVIT 361

Query: 420 DAFTEAGLVLEKDAKQKLKLHATVMNARHRKSKK--KKKFDSFDAREIFKQYGSEEWGEY 479
           DAF +AGLVLEKDAKQ LKLH TVMNARHRK +K  KKK ++FDAREI KQ+G+E+WGEY
Sbjct: 362 DAFVKAGLVLEKDAKQSLKLHVTVMNARHRKRRKNNKKKMETFDAREIHKQFGNEDWGEY 407

Query: 480 HIREAHLSQRFVFDENGYYHCCASIPFPDEQ 507
            I+EAHLSQRFVFD+NGYY CC SIPFP EQ
Sbjct: 422 LIQEAHLSQRFVFDQNGYYRCCGSIPFPGEQ 407

BLAST of PI0022458 vs. TAIR 10
Match: AT3G16220.1 (Predicted eukaryotic LigT )

HSP 1 Score: 239.6 bits (610), Expect = 5.6e-63
Identity = 136/277 (49.10%), Postives = 172/277 (62.09%), Query Frame = 0

Query: 227 YSHFVSLPLAIHPELVEKLINFQNSILRSSESCLDEAEDSDTNEDNTDNELEVQHTVKAP 286
           ++HFVSLPLAI+P+L + +  FQNS+L                 +N  + L+ Q      
Sbjct: 36  FTHFVSLPLAIYPDLTKNIEAFQNSVL----------------GNNDKDPLKFQ------ 95

Query: 287 DVAVELQVDDKREQIKVNINIPVVSYPPKTSKVSTPSDLGIDKSIFIKPKTFHLTVLMLK 346
                                            ST +++GI+KSIF+ PKTFHLTV+MLK
Sbjct: 96  ---------------------------------STLAEMGIEKSIFVTPKTFHLTVVMLK 155

Query: 347 LWNKERVDAASEVLRGISSKVVDALDNRPVLIKLKGLDCMRGSLAKARVLYAPVEEIGDE 406
           L N E V  A  +L+ I S V  AL NRPV I+L+GL+CM GSL K RVLYAPVEE+G E
Sbjct: 156 LENNESVVKAQNILQSIFSNVRQALKNRPVFIRLRGLECMSGSLDKTRVLYAPVEEVGRE 215

Query: 407 GRLLRACQVIIDAFTEAGLVLEKDAKQKLKLHATVMNARHRKSKKKKKFDSFDAREIFKQ 466
           GRLL AC VIIDAF   G    KDAK +LKLHAT+MNA +RK  K KK D+FDAREI K+
Sbjct: 216 GRLLNACHVIIDAFENVGFA-GKDAKSRLKLHATLMNASYRKD-KSKKMDTFDAREIHKE 255

Query: 467 YGSEEWGEYHIREAHLSQRFVFDENGYYHCCASIPFP 504
           + +++WG Y IREAH+SQR+ +D NGY+HCCAS+PFP
Sbjct: 276 FENKDWGTYLIREAHISQRYKYDPNGYFHCCASLPFP 255