PI0022427 (gene) Melon (PI 482460) v1

Overview
NamePI0022427
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr11: 1318772 .. 1324246 (-)
RNA-Seq ExpressionPI0022427
SyntenyPI0022427
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAAAACGAAAGTGCAAAAATTTGTGTAGCAGTTTACAAGAAATATGGAAGAGGAGCAGTTTTTGCCCATCGGCTGCCATTCGGCAATTAATAAAGATGTGATAATTAAGATGATGAAGAATCTCATTGAACCGAAGGGAACCGAAGAATCAATTGCGGTAAGTGTGGTTGGATTATCATTAAGAAGCGACACCCAACCTTCAATCTACAAAATACCAGAATTCATGAGAGGACTTCAAGTCAAAGCTTTCGAGCCACAGGTGATATCGTTTGGGCCATACCACTATGGGAAAGATCATTTGGCTAGAATGGAACGTGAAAAAAAGAAAGCATTTCAGCGTTTGTTATGGCGCAGCAAAGTAAATTGCGAGTTGATTTTGGACAATGTGTGTAAGATTTTGGAAGATCTGTATGGAGCATATGATAATCTTGATGAGAAATGGAAGAAGGATGCTACCAAGTTCATCGAGATTATGATTATGGATGGGTGTTTCATGCTGGATTTCTTCATTAATTTCTGTCCTGAATCCAATTCGCTATTTACTATGCAAAAACATATAAAGCGAGACATGTTGCTGCTCGAGAACCAGCTACCTTTCCAGCTTCTTGAGCAGTTGTATGACATGATATTACCAAACGAGGTATTCTTTAATTTATCTCTCTTTACTTATCTTACTCCTTGGCTATATTTTATACGACAATTGCCATAGGGGTAGATGGATACTACATCTTTTAAGAATTGTAAATATACTAAAAAGTTATTAGTGATCCACTTAATTACATGCCAATTTTTTACATGCTATACTAAATTTGCTAATATTTTGGCATTATTATTATATTTGTAACTATCTTTATTTTGTTTCATTTAGGTTGATTTTGTTTTTATAAATTAATTAAATCTTTAAAATAATCCACTTTCAATAAGTCCTTCAATTTTAAATTATATGTCAAAGCTCACTTTTTTTTTTTTTAATTTACAACCTACTAAACACAAGATTGAAACTTTATAATCTTATCGTACAAAAAAAGTTTTGGATATTTGCATGATTTTTGTTTTTGTTGGTTTTTTTGTTTCATTTGAAGGGTTTAATTATTAGGCATAAGTGTGATGTAGCGGAAATGTTGTTTAGGCCAAATTCATGACCAAACAAGGAAAATCACTCCCAAAAGATGCAAAGCTCTCAAAAAAACAAAGAAATATTGATCAAAAGTTCCATAATTACAACCATAGGATGTCTATTTATTGGCTTACAAGGAATTAACATAATTAACTAATTAATTTTAATTATGTACTAATTAATCTAAATTAACAACTTAAACAAATATTAAACTAGCTATACTAATTTTTTTGATTCCCTCATCAGTATGTTACCGGCAATTTTAGCATAGTTATATGCGGTAGAGAAACCTGAAATAATTTGTAGATTGTTATTATATAGCGAAAAATATTTAAATTTTTTATTCTATATCTAATTAATTTTTAATTTCAGACACTTGTGAGGTAGATAAAATTGAAAGTTTAAAGACCAACACTTTTGAAAGCAATTCACTTCACTTTTCTTTCCAAACAAGCTTGAGTGTTTATTATGGATTACACTTTGTTATGACACCAATTCTAATCTCATGGGAAAGCCTAAAAAAGTTTAGAGAGAGCTCGGTTGGTCCTATGAGGTAAAACTCAATGAAACTTTGTTTGGAACTCAAGAGGACACTTGGGGAGAGTCCTCAATAGAACCCACGCAATAGAACTTAGGAGAGAGCTCAAGATAACCATGGTGGGTGAGCCGAAGAGAGCTTGATAGGATTTATTAGGTAAGCTTTTTGGAACACTTAGGACCCATGGGTATCAATGAGCCTACGAGAATGTAGAGAGAGCCTTAGAGAGACCCATGAGGAGAGTTTGGTGGAGTTTAGAATAATAGAAGAGATAAAAGCATCCCCAACCTTTGGCAAGACACAGGCTCTCTAACTGAATGTTGGACCTACAAATATATCTTATTCAAGGATTACAACCCTACCTATATTGCTCTCAATCTTAATGCACTCTGTAGTATATAATCTGTAGGTTTACCTCTAGCCTATGGACTTTTGTACTTTGTTCTTGCTTTAATGAAATATCTCATGAAAGTTTTTGGGGTGTCTTTACTCTCCGACCGTATCTTTATTTCAAAAAGAAGGAGGACCACAAAAGAGAACCTAGCCAAACTCTTGTAGAGGAGCTCACAAGGTGGGGGAGAGCTCGCTAAGGAAATCTTAGCATTACATGGGAGAGTCTTAGCCTCCTAAGAGGAGCTCACAAGAGAGGATGAGGACCCAAAGATTGCAAAGAAAAACCTAGCTTCCTTAGAAGAGCTCACAAGAGGGATCCACAAGAGAGAGTCTAGATGAGAGATGGGGTCTAGCCAGAGAAGGAGAACTCACAAGAAGGAGCCTGCAAGGGAGAACCTAGGTCTTCTAAGAGGAGCTGCCCAACGACTCATAATGGAGAGTCTAGCCTCCTAAGAGGAGCTAATGAAGCAAAAAGTTGTTGAGGCTTTTGATGAGATCAAATAATAGTATTAGTGTAGTAACAGTCGATGGTTAAAGAGCTGAATAATTTTCCTTTCAAAATACTAATTGGTAGGTAACCAAAGGGTCGATTTAAATTGATTTTGATTGCTTGAATTATTTTGAAAAGCGGTTGACAAGAATAGAATTGAAGATTGATTTGGATTGAAACTATCAATGACACAAAATATCTAGCCCGGGTATAATGAAGTTACTCTTAATTTCCTACTCAATCATCAATTAATCATTACACATTTAGAAAAGTTAAATGGGATGTCCTAATAATCAAACTATTCTTAGGTTTAGATGAAAAACATATTCATAAACATGCAAATCAATTACCATTAAGATTTAGGGTTATAGCTAAGACTAACAAAATAATATGGAATATTGCCTAAATTAAATGTGGGATACAAATCTTACTAAGTTAGTTAGACAAATGTTATTGGAATAGAATTGTGGAATACAACTGTAGCTAACTTAACCTTTGAATTTGTGTAAGTGATTAATGTTGTATAGGTGAGCGTAGTAGAGACCAAACTTCGAATTTGTGTAAGTGATTAACAAGTAGCGAAAAACATGCAGATTTTTACTTAATCTCATGCAACCCATTTTCTTACCTAATAGATCTTTTTGTTGATTTTTATTGGGTAACTACACTAGGTGCACCAGGGTTTGAATCACATCTAACTATTATTAAATGTAGTACTGCATTATCATTGAATAAGTTATTGTTGAATGAAATTAAATAATATATATCACAATAAAGCCACATCCTAATTAATAATTAATAAATTATAAACCGCTAGGTTAGATGTGAATTCAAAAACTTGGATGTGCACCCTCCCTAACACGTGCATGTCTTCTACTTCTATTTTTTTTTTTTTTTTTTAACATCTCATATATAAGATATATACATTGGAAGAATCAATACATCCTACCTATCCAATATAAGTAACTACTTCTATCCCGAACAAATGCATTTGACATCTATTCCATTTTATCTCGAACGGTAGTGTTCTGTCAATATCAATTACACTCTAAAAATCACTTTTCTGTCTTTTAAATGGACACTTACATTAAAAAAAAAATCACAAACATTAGAATTCATACAAAATATAGCGACAAAATGAATATGTAAAGATTGATCCCTAAAATAATTTGATCATTTGCATGGGTTTTGGTTCTACAGGATCGACATGAACCTCTGAGATCCCTTGTTTGCCGATCAACATCGTTCATCAACCGTGATCGGATATGGAAAAGAAAAATATGGCACATTTTGGACATGTATAGAGATTCATTATTGAATCCACCTATAAGAAGTTATCGTAGAAAAGAATATGTCGACGACGATTTTGACGATCTTGACCACGACCAATTCGGCCTAGACCGTCAAATAATTCCACAAGCAATAGAGCTTCGTAAGGCAGGGATAAAATTCAAGAAAAGCCAAACATCTAGCCTTTCAGACGTGAATTTTAACTCAAAACTAGGCGTGTTGGAAATGCCATATCTAGCTGTGAACAATGATACTGAAACTACTTTATTAAATGTGATGGCATTTGAGAAACTGATGGGAGATGAAAGTACAGTAATTTCTTTTGTGACGTTGATAACAAATCTAATACAAGAGGAAAAAGATGTGTCAGTGCTGACTTCCAATGGAATATTGGGGAATGGCCTTCAAAACAGCAAACGTGTGGTGGAGTTGTTCAATCTGCTGGCCAAAGGACCGGCTACTGAGTTGAATGACCACATTAGTGAAACATTATCGATGGTGAATAAGCATTGTAACAGTTCGTGGAAAATGTGGTGGGCAAATCTCAGAGACCCTGAAATCAAAATCCATGGGTTATTGTCTCCATCGCTGCTGCTTCCTTTGGTTTTCTCCTCCAAATTATCCAAACCTTCTACGGAGTATATGGAATTTACCACCCAAATCATTAACATATATAACTTATATATACATAAATTAAAATCCACTCCATTCCAATCCCTCTCTTTTCTTTTTCTTTTTTTTTTTTGTGGAAAAATTAATATTATAAACAACAAATCCCCTCCATTTCAATTTGCATTTTGAATCCCCATCCATATTTATTAATCAATTCGATCTTAACATATCTTTCTATCAAATGGGTTGAAACTCAAATGAAAATCTCTTCCTCATACATTTTTTTCCTTCCATATTATAATTATGAATTGAAGTGTATATATGTGTGCTCTTGCCCAATATATGTCTGCGCTATTGATATATATATGATTGATATTGTTATTATTTGATACTAAATATTTTGTGATTATTATCTCATTTGGTTGCTATTTAATACTAAGTAAAGCTTGGAATTGTTATTTGATTGCTATTCGATACTAATAAGTACTTTGTGTTTGCTATCTAATTCCTAATTGCTATTTAATTTTAAAGTAATTTCTATTTGATATGAAGAGTAGTTTGCAAACCAAATTAAAAAGCAACACAAGATAAGTGTCAAGTGGATAATGAACCGACCAAAAAAAAAATTGCAACTCACACTTAAGGCTTCTAATTTGAAATTTGTTCTGTTTTTTATATCTTGAGACTACTCACACTGCATAATATATGACTAGAGATGTCTGATCTGCTATGTATATATAAAATTTCTTTATGTCAGCGTACATGACTAGAGATTTATGTATAGATTTGATGATGATTTTTTTTCACAAACCTATATATAAGTTTCCAAGAGCTATTCTTATGGAATATTTCTCGATGTGTCAATTATTTCAACATGAGTTACATGATTTTTCTAGAGTTTTAAAATCTGTAATCAAAATTAGTATATTTTTCTTATTAAACTTCATCACTCACTGGATTTTTTTAGCCATTTTTTATTCTTAAGCTTTTCCACTAGGCAGCATTTGAGTTTCTGACGAGCTTATGGAGTTGA

mRNA sequence

GACAAAACGAAAGTGCAAAAATTTGTGTAGCAGTTTACAAGAAATATGGAAGAGGAGCAGTTTTTGCCCATCGGCTGCCATTCGGCAATTAATAAAGATGTGATAATTAAGATGATGAAGAATCTCATTGAACCGAAGGGAACCGAAGAATCAATTGCGGTAAGTGTGGTTGGATTATCATTAAGAAGCGACACCCAACCTTCAATCTACAAAATACCAGAATTCATGAGAGGACTTCAAGTCAAAGCTTTCGAGCCACAGGTGATATCGTTTGGGCCATACCACTATGGGAAAGATCATTTGGCTAGAATGGAACGTGAAAAAAAGAAAGCATTTCAGCGTTTGTTATGGCGCAGCAAAGTAAATTGCGAGTTGATTTTGGACAATGTGTGTAAGATTTTGGAAGATCTGTATGGAGCATATGATAATCTTGATGAGAAATGGAAGAAGGATGCTACCAAGTTCATCGAGATTATGATTATGGATGGGTGTTTCATGCTGGATTTCTTCATTAATTTCTGTCCTGAATCCAATTCGCTATTTACTATGCAAAAACATATAAAGCGAGACATGTTGCTGCTCGAGAACCAGCTACCTTTCCAGCTTCTTGAGCAGTTGTATGACATGATATTACCAAACGAGGATCGACATGAACCTCTGAGATCCCTTGTTTGCCGATCAACATCGTTCATCAACCGTGATCGGATATGGAAAAGAAAAATATGGCACATTTTGGACATGTATAGAGATTCATTATTGAATCCACCTATAAGAAGTTATCGTAGAAAAGAATATGTCGACGACGATTTTGACGATCTTGACCACGACCAATTCGGCCTAGACCGTCAAATAATTCCACAAGCAATAGAGCTTCGTAAGGCAGGGATAAAATTCAAGAAAAGCCAAACATCTAGCCTTTCAGACGTGAATTTTAACTCAAAACTAGGCGTGTTGGAAATGCCATATCTAGCTGTGAACAATGATACTGAAACTACTTTATTAAATGTGATGGCATTTGAGAAACTGATGGGAGATGAAAGTACAGTAATTTCTTTTGTGACGTTGATAACAAATCTAATACAAGAGGAAAAAGATGTGTCAGTGCTGACTTCCAATGGAATATTGGGGAATGGCCTTCAAAACAGCAAACGTGTGGTGGAGTTGTTCAATCTGCTGGCCAAAGGACCGGCTACTGAGTTGAATGACCACATTAGTGAAACATTATCGATGGCAGCATTTGAGTTTCTGACGAGCTTATGGAGTTGA

Coding sequence (CDS)

ATGGAAGAGGAGCAGTTTTTGCCCATCGGCTGCCATTCGGCAATTAATAAAGATGTGATAATTAAGATGATGAAGAATCTCATTGAACCGAAGGGAACCGAAGAATCAATTGCGGTAAGTGTGGTTGGATTATCATTAAGAAGCGACACCCAACCTTCAATCTACAAAATACCAGAATTCATGAGAGGACTTCAAGTCAAAGCTTTCGAGCCACAGGTGATATCGTTTGGGCCATACCACTATGGGAAAGATCATTTGGCTAGAATGGAACGTGAAAAAAAGAAAGCATTTCAGCGTTTGTTATGGCGCAGCAAAGTAAATTGCGAGTTGATTTTGGACAATGTGTGTAAGATTTTGGAAGATCTGTATGGAGCATATGATAATCTTGATGAGAAATGGAAGAAGGATGCTACCAAGTTCATCGAGATTATGATTATGGATGGGTGTTTCATGCTGGATTTCTTCATTAATTTCTGTCCTGAATCCAATTCGCTATTTACTATGCAAAAACATATAAAGCGAGACATGTTGCTGCTCGAGAACCAGCTACCTTTCCAGCTTCTTGAGCAGTTGTATGACATGATATTACCAAACGAGGATCGACATGAACCTCTGAGATCCCTTGTTTGCCGATCAACATCGTTCATCAACCGTGATCGGATATGGAAAAGAAAAATATGGCACATTTTGGACATGTATAGAGATTCATTATTGAATCCACCTATAAGAAGTTATCGTAGAAAAGAATATGTCGACGACGATTTTGACGATCTTGACCACGACCAATTCGGCCTAGACCGTCAAATAATTCCACAAGCAATAGAGCTTCGTAAGGCAGGGATAAAATTCAAGAAAAGCCAAACATCTAGCCTTTCAGACGTGAATTTTAACTCAAAACTAGGCGTGTTGGAAATGCCATATCTAGCTGTGAACAATGATACTGAAACTACTTTATTAAATGTGATGGCATTTGAGAAACTGATGGGAGATGAAAGTACAGTAATTTCTTTTGTGACGTTGATAACAAATCTAATACAAGAGGAAAAAGATGTGTCAGTGCTGACTTCCAATGGAATATTGGGGAATGGCCTTCAAAACAGCAAACGTGTGGTGGAGTTGTTCAATCTGCTGGCCAAAGGACCGGCTACTGAGTTGAATGACCACATTAGTGAAACATTATCGATGGCAGCATTTGAGTTTCTGACGAGCTTATGGAGTTGA

Protein sequence

MEEEQFLPIGCHSAINKDVIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPSIYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILDNVCKILEDLYGAYDNLDEKWKKDATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQKHIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHILDMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGDESTVISFVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISETLSMAAFEFLTSLWS
Homology
BLAST of PI0022427 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.4e-23
Identity = 100/362 (27.62%), Postives = 182/362 (50.28%), Query Frame = 0

Query: 54  IYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILD 113
           I+++PE    L  KA++P+V+S GPYHYG+ HL  +++ K +  Q  L  +K   + + +
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKK--KDVEE 107

Query: 114 NVCKILEDLYGAYDNLDEKWKKDATKFIE-------IMIMDGCFMLDFFI----NFCPES 173
           NV      L  A  +L++K +K  ++ ++       +M++DGCF+L  F+    N     
Sbjct: 108 NV------LVKAVVDLEDKIRKSYSEELKTGHDLMFMMVLDGCFILMVFLIMSGNIELSE 167

Query: 174 NSLFT---MQKHIKRDMLLLENQLPFQLLEQLY--DMILPNEDRHEP----LRSLVCRST 233
           + +F+   +   I+ D+LLLENQ+PF +L+ LY    I  + D +       ++ + +  
Sbjct: 168 DPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFFKNPIDKEG 227

Query: 234 SFINRDRIWKRKIWHILDMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFG----LDRQI 293
           S+  + R +K K  H+LD+ R++ L  P  S   K         L   + G    +D + 
Sbjct: 228 SYWEKHRNYKAK--HLLDLIRETFL--PNTSESDKASSPHVQVQLHEGKSGNVPSVDSKA 287

Query: 294 IP---QAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEK 353
           +P    A  LR  GIKF+  ++   S +N   K   L++P L  +    +  LN +AFE+
Sbjct: 288 VPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFEQ 347

Query: 354 LMGDEST-VISFVTLITNLIQEEKDVSVLTSNG-ILGNGLQNSKRVVELFNLLAKGPATE 387
              D S  + +++  +  L+  E+DV+ L ++  I+ N   ++  V E F  ++K    E
Sbjct: 348 FYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKDVVFE 397

BLAST of PI0022427 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 8.0e-09
Identity = 35/111 (31.53%), Postives = 64/111 (57.66%), Query Frame = 0

Query: 270 IPQAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMG 329
           IP   +L KAG++FK +   ++S V F+S  G   +P + ++ +TET L N++A+E    
Sbjct: 345 IPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNT 404

Query: 330 DESTVIS-FVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAK 380
               V + +  LI  +I  E+DV +L   G+L + L++ +   E++N ++K
Sbjct: 405 SGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSK 455

BLAST of PI0022427 vs. ExPASy TrEMBL
Match: E5GB49 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 5.9e-79
Identity = 187/407 (45.95%), Postives = 263/407 (64.62%), Query Frame = 0

Query: 2   EEEQFLPIGC------HSAINKDVIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPSIY 61
           EEE  LPI C         ++K+ ++  +K+ ++    +  + V+    S     +PSIY
Sbjct: 6   EEETPLPISCINDHKDQFPMDKNDVVINVKDCLKELLMKRPVPVN-ESPSSEKTIKPSIY 65

Query: 62  KIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLW--RSKVNCELILD 121
           KIP+FM+ +Q+KA+EP ++SFGPYH+G +HLA ME+EK+K FQ L+    +    E I  
Sbjct: 66  KIPKFMKDIQLKAYEPYLVSFGPYHHGVEHLAPMEKEKQKVFQHLVKGDNNAATYESIAS 125

Query: 122 NVCKILEDLYGAYDNLDEKWKKD---ATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQK 181
            V  ILEDLY AYDNLDEKW+KD   + KF+E+MI+D CF+L FF +      SL T++ 
Sbjct: 126 EVSNILEDLYAAYDNLDEKWRKDVVASAKFMEMMIIDACFILVFF-SKDKSYKSLMTLRS 185

Query: 182 HIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHIL 241
            IKRD+LLLENQLPFQLL+ LY  ILP +D+++ L SL+C+   F+ +D +  +   HIL
Sbjct: 186 DIKRDILLLENQLPFQLLQLLY-KILPIKDQNKSLTSLICK-LWFVKKDELTVKGGKHIL 245

Query: 242 DMYRDSLLNP-PI----RSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKK 301
           +MYR  LL+P PI    R   +K+      +  + D+  L+ QIIPQA  L  AGIKF++
Sbjct: 246 EMYRMLLLDPIPIVLSERDESQKKAEGTKGNKKEKDESVLNSQIIPQATLLHDAGIKFRR 305

Query: 302 SQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGD-ESTVISFVTLITNL 361
           S+T SL D+ F  K GVLE+P+L V++DTET LLNVMAFEKL G+  S V SFV L+ NL
Sbjct: 306 SETESLIDIGF--KNGVLELPHLTVDDDTETKLLNVMAFEKLHGNVRSYVTSFVVLMNNL 365

Query: 362 IQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISE 392
           I  +KDV +L+ N I+ N L N +   +LF +L KG A +L  +I++
Sbjct: 366 IDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGKGVALDLESNIAK 406

BLAST of PI0022427 vs. ExPASy TrEMBL
Match: A0A1S4E2L7 (uncharacterized protein LOC103499077 OS=Cucumis melo OX=3656 GN=LOC103499077 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 5.9e-79
Identity = 187/407 (45.95%), Postives = 263/407 (64.62%), Query Frame = 0

Query: 2   EEEQFLPIGC------HSAINKDVIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPSIY 61
           EEE  LPI C         ++K+ ++  +K+ ++    +  + V+    S     +PSIY
Sbjct: 482 EEETPLPISCINDHKDQFPMDKNDVVINVKDCLKELLMKRPVPVN-ESPSSEKTIKPSIY 541

Query: 62  KIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLW--RSKVNCELILD 121
           KIP+FM+ +Q+KA+EP ++SFGPYH+G +HLA ME+EK+K FQ L+    +    E I  
Sbjct: 542 KIPKFMKDIQLKAYEPYLVSFGPYHHGVEHLAPMEKEKQKVFQHLVKGDNNAATYESIAS 601

Query: 122 NVCKILEDLYGAYDNLDEKWKKD---ATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQK 181
            V  ILEDLY AYDNLDEKW+KD   + KF+E+MI+D CF+L FF +      SL T++ 
Sbjct: 602 EVSNILEDLYAAYDNLDEKWRKDVVASAKFMEMMIIDACFILVFF-SKDKSYKSLMTLRS 661

Query: 182 HIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHIL 241
            IKRD+LLLENQLPFQLL+ LY  ILP +D+++ L SL+C+   F+ +D +  +   HIL
Sbjct: 662 DIKRDILLLENQLPFQLLQLLY-KILPIKDQNKSLTSLICK-LWFVKKDELTVKGGKHIL 721

Query: 242 DMYRDSLLNP-PI----RSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKK 301
           +MYR  LL+P PI    R   +K+      +  + D+  L+ QIIPQA  L  AGIKF++
Sbjct: 722 EMYRMLLLDPIPIVLSERDESQKKAEGTKGNKKEKDESVLNSQIIPQATLLHDAGIKFRR 781

Query: 302 SQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGD-ESTVISFVTLITNL 361
           S+T SL D+ F  K GVLE+P+L V++DTET LLNVMAFEKL G+  S V SFV L+ NL
Sbjct: 782 SETESLIDIGF--KNGVLELPHLTVDDDTETKLLNVMAFEKLHGNVRSYVTSFVVLMNNL 841

Query: 362 IQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISE 392
           I  +KDV +L+ N I+ N L N +   +LF +L KG A +L  +I++
Sbjct: 842 IDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGKGVALDLESNIAK 882

BLAST of PI0022427 vs. ExPASy TrEMBL
Match: A0A5A7TA99 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001800 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 5.0e-70
Identity = 173/370 (46.76%), Postives = 237/370 (64.05%), Query Frame = 0

Query: 34  EESIAVSVVGLSLRSDTQPSIYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREK 93
           EESI +    LS +   +PSIYKIP+F+R +  KA+EP+++S GPYHYGK HL  MEREK
Sbjct: 16  EESIGMEEF-LSTQHMIKPSIYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLLPMEREK 75

Query: 94  KKAFQRLLWRSKVNCELILDNVCKILEDLYGAY-DNLDEKWKKDATKFIEIMIMDGCFML 153
            KAF     R+ +  E I+D+VC ILEDL G+Y DNLD+ WK+D   F+++MI+DGCF+L
Sbjct: 76  YKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDIWKEDVAMFLKLMIVDGCFVL 135

Query: 154 DFFINFCPESNSLFTMQKHIKRDMLLLENQLPFQLLEQLYDM---ILPNEDRHEPLRSLV 213
           D       +S     M   IKRDM+LLENQLP QLL+QL+ +      NE+ H  L +++
Sbjct: 136 DLISELSSKSLGR-CMIWDIKRDMVLLENQLPLQLLKQLHALTTTTTENENEHLDLEAMI 195

Query: 214 CRSTSFINRDRIWKRKIWHILDMYRDSLLNPPI-RSYRRKEYVDDDFDDLDHDQFGLDRQ 273
             S   ++ + +  R   HILDMYR SLL+P I +     E +    +    ++  ++ Q
Sbjct: 196 W-SWMRLSGEILSMRNPLHILDMYRSSLLSPTICKQDETHENMIAILEWTLTNEDNVECQ 255

Query: 274 IIPQAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNND-TETTLLNVMAFEKL 333
            I  A +LRKAGIKF+KS T++L DV+F+ K  VL +P LA+++  TE TL+N+MAFEKL
Sbjct: 256 SIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKL 315

Query: 334 -MGDESTVISFVTLITNLIQEEKDVSVLTSNGIL-GNGLQNSKRVVELFNLLAKGPATEL 393
            +G E  V SFV L+ NLI  +KDVS+L S GIL  N + +   VV+LFNLLAKG    L
Sbjct: 316 HVGVERQVTSFVVLMKNLIGIDKDVSLLASKGILSSNVIHDGNGVVQLFNLLAKGQTKYL 375

Query: 394 NDHISETLSM 396
            +H+ E   M
Sbjct: 376 ENHMCELFKM 382

BLAST of PI0022427 vs. ExPASy TrEMBL
Match: A0A6J1KVQ6 (UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 9.4e-69
Identity = 168/368 (45.65%), Postives = 233/368 (63.32%), Query Frame = 0

Query: 27  LIEPKGTEESIAVSVVGLSLRSDTQP---SIYKIPEFMRGLQVKAFEPQVISFGPYHYGK 86
           ++  KGT + +  S   L++ ++      SIYKIP FM     KA+EPQV+S GPY++GK
Sbjct: 24  VVRVKGTLDQLLDSPAILNMEAEQSSELLSIYKIPFFMTQTHPKAYEPQVVSLGPYNHGK 83

Query: 87  DHLARMEREKKKAFQRLLWRSKVNCELILDNVCKILEDLYGAYDNLDEKWKKDATKFIEI 146
            HL+ ME EK K F     R  ++ E I+  V  IL++L  +YD L+E+W +D  KF+++
Sbjct: 84  QHLSPMELEKLKLFHSFKTRCLLDVESIVRGVSTILDELMESYDRLEEEWTQDPGKFLQL 143

Query: 147 MIMDGCFMLDFFINFCPESNSLFTMQKHIKRDMLLLENQLPFQLLEQLYDMILPNEDRHE 206
           MI+DGCFML F I+ CP  NSL  +   IK+DMLLLENQLP  LLE+LY +   N    +
Sbjct: 144 MIVDGCFMLGFLIS-CP--NSLINVSPDIKQDMLLLENQLPMLLLEKLYSIAGRNVQLPQ 203

Query: 207 PLRSLVCRSTSFINRDRIWKRKIWHILDMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQF 266
             + LVC+  S I ++ + K  + HIL+MY++SLL PPI   RR     D   +LDH   
Sbjct: 204 DPKKLVCKWLS-IPQNEVMKDCL-HILEMYKESLLYPPID--RR-----DWSAELDHSD- 263

Query: 267 GLDRQIIPQAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMA 326
             + Q+IP A +L +AGIKFK+S+T SL DV F++K GVL +P L V++DTE+T+LNVMA
Sbjct: 264 -PECQVIPPATKLHEAGIKFKRSKTESLRDVWFDTKRGVLWLPQLMVDDDTESTMLNVMA 323

Query: 327 FEKL-MGDESTVISFVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPA 386
           FEKL M     V SFV L++NLI +E+DV+VL    IL N + N K    LF+ L  G A
Sbjct: 324 FEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGEKILANAVGNDKEAAGLFSRLGSGAA 377

Query: 387 TELNDHIS 391
             L+ H++
Sbjct: 384 MGLDSHMA 377

BLAST of PI0022427 vs. ExPASy TrEMBL
Match: A0A6J1KYV8 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 4.7e-68
Identity = 170/373 (45.58%), Postives = 235/373 (63.00%), Query Frame = 0

Query: 27  LIEPKGTEESIAVSVVGLSLRSDTQP---SIYKIPEFMRGLQVKAFEPQVISFGPYHYGK 86
           ++  KGT + +  S   L++ ++      SIYKIP FM     KA+EPQV+S GPY++GK
Sbjct: 24  VVRVKGTLDQLLDSPAILNMEAEQSSELLSIYKIPFFMTQTHPKAYEPQVVSLGPYNHGK 83

Query: 87  DHLARMEREKKKAFQRLLWRSKVNCELILDNVCKILEDLYGAYDNLDEKWKKDATKFIEI 146
            HL+ ME EK K F     R  ++ E I+  V  IL++L  +YD L+E+W +D  KF+++
Sbjct: 84  QHLSPMELEKLKLFHSFKTRCLLDVESIVRGVSTILDELMESYDRLEEEWTQDPGKFLQL 143

Query: 147 MIMDGCFMLDFFINFCPESNSLFTMQKHIKRDMLLLENQLPFQLLEQLYDMI-----LPN 206
           MI+DGCFML F I+ CP  NSL  +   IK+DMLLLENQLP  LLE+LY +      LP 
Sbjct: 144 MIVDGCFMLGFLIS-CP--NSLINVSPDIKQDMLLLENQLPMLLLEKLYSIAGRNVQLPQ 203

Query: 207 EDRHEPLRSLVCRSTSFINRDRIWKRKIWHILDMYRDSLLNPPIRSYRRKEYVDDDFDDL 266
           +D     + LVC+  S I ++ + K  + HIL+MY++SLL PPI   RR     D   +L
Sbjct: 204 QDP----KKLVCKWLS-IPQNEVMKDCL-HILEMYKESLLYPPID--RR-----DWSAEL 263

Query: 267 DHDQFGLDRQIIPQAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTL 326
           DH     + Q+IP A +L +AGIKFK+S+T SL DV F++K GVL +P L V++DTE+T+
Sbjct: 264 DHSD--PECQVIPPATKLHEAGIKFKRSKTESLRDVWFDTKRGVLWLPQLMVDDDTESTM 323

Query: 327 LNVMAFEKL-MGDESTVISFVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLL 386
           LNVMAFEKL M     V SFV L++NLI +E+DV+VL    IL N + N K    LF+ L
Sbjct: 324 LNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGEKILANAVGNDKEAAGLFSRL 378

Query: 387 AKGPATELNDHIS 391
             G A  L+ H++
Sbjct: 384 GSGAAMGLDSHMA 378

BLAST of PI0022427 vs. NCBI nr
Match: XP_038875622.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 349.7 bits (896), Expect = 3.3e-92
Identity = 203/397 (51.13%), Postives = 266/397 (67.00%), Query Frame = 0

Query: 1   MEEEQFLPIGCHSAINK-----DVIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPSIY 60
           MEE+  LPI      N+     DV+I++  NL       +S AV  +G  L   TQPSIY
Sbjct: 1   MEEQPLLPIRHIRNDNQVGKKDDVVIQVKNNL---NKLLDSAAVDEMG-PLGESTQPSIY 60

Query: 61  KIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILDNV 120
           KIPEFMR +Q KAFEPQ++S GPYH+GK+HL  ME EK+KAF+R +    +N E I++++
Sbjct: 61  KIPEFMRKIQAKAFEPQLVSLGPYHHGKEHLVSMEEEKQKAFRRFVNDYGLNLESIVESM 120

Query: 121 CKILEDLYGAYDNLDEKWKKDATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQKHIKRD 180
              LE+L+GAYD LDEKWK+D  KF+E+MI+DGCFMLDFF   CP   SL TM+  IKRD
Sbjct: 121 SNNLENLWGAYDKLDEKWKQDGAKFLEVMIVDGCFMLDFF-KECP--YSLSTMRWDIKRD 180

Query: 181 MLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHILDMYRD 240
           MLLLENQLP QLL++LYD +       + LRSL+C+    IN + +  R++ HILDMYR 
Sbjct: 181 MLLLENQLPMQLLQELYDNMPNTNQEDQSLRSLICQPMC-INEEAVMGREL-HILDMYRA 240

Query: 241 SLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKKSQTSSLSDVN 300
           SLL PP+    R          + + Q   + QIIPQA +L  AGIKFK S T +L+DV+
Sbjct: 241 SLLYPPVDRKDRSGKT-----KVTYQQSDPECQIIPQATQLHDAGIKFKSSPTKNLTDVS 300

Query: 301 FNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGDE-STVISFVTLITNLIQEEKDVSVL 360
           FN K GVLE+PY+ V++DTETTLLNVMAFEKL  +  S V SFV L+ NLI  ++DV++L
Sbjct: 301 FNPKQGVLELPYIVVDDDTETTLLNVMAFEKLYVEAGSKVTSFVILMNNLIDVDRDVALL 360

Query: 361 TSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISE 392
            S+ IL N L N +   +LF+LL KG A +L+ HI++
Sbjct: 361 ASDKILANALGNDESAADLFSLLGKGAAMDLDSHITD 383

BLAST of PI0022427 vs. NCBI nr
Match: XP_016902479.1 (PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo])

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-78
Identity = 187/407 (45.95%), Postives = 263/407 (64.62%), Query Frame = 0

Query: 2   EEEQFLPIGC------HSAINKDVIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPSIY 61
           EEE  LPI C         ++K+ ++  +K+ ++    +  + V+    S     +PSIY
Sbjct: 482 EEETPLPISCINDHKDQFPMDKNDVVINVKDCLKELLMKRPVPVN-ESPSSEKTIKPSIY 541

Query: 62  KIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLW--RSKVNCELILD 121
           KIP+FM+ +Q+KA+EP ++SFGPYH+G +HLA ME+EK+K FQ L+    +    E I  
Sbjct: 542 KIPKFMKDIQLKAYEPYLVSFGPYHHGVEHLAPMEKEKQKVFQHLVKGDNNAATYESIAS 601

Query: 122 NVCKILEDLYGAYDNLDEKWKKD---ATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQK 181
            V  ILEDLY AYDNLDEKW+KD   + KF+E+MI+D CF+L FF +      SL T++ 
Sbjct: 602 EVSNILEDLYAAYDNLDEKWRKDVVASAKFMEMMIIDACFILVFF-SKDKSYKSLMTLRS 661

Query: 182 HIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHIL 241
            IKRD+LLLENQLPFQLL+ LY  ILP +D+++ L SL+C+   F+ +D +  +   HIL
Sbjct: 662 DIKRDILLLENQLPFQLLQLLY-KILPIKDQNKSLTSLICK-LWFVKKDELTVKGGKHIL 721

Query: 242 DMYRDSLLNP-PI----RSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKK 301
           +MYR  LL+P PI    R   +K+      +  + D+  L+ QIIPQA  L  AGIKF++
Sbjct: 722 EMYRMLLLDPIPIVLSERDESQKKAEGTKGNKKEKDESVLNSQIIPQATLLHDAGIKFRR 781

Query: 302 SQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGD-ESTVISFVTLITNL 361
           S+T SL D+ F  K GVLE+P+L V++DTET LLNVMAFEKL G+  S V SFV L+ NL
Sbjct: 782 SETESLIDIGF--KNGVLELPHLTVDDDTETKLLNVMAFEKLHGNVRSYVTSFVVLMNNL 841

Query: 362 IQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISE 392
           I  +KDV +L+ N I+ N L N +   +LF +L KG A +L  +I++
Sbjct: 842 IDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGKGVALDLESNIAK 882

BLAST of PI0022427 vs. NCBI nr
Match: ADN33691.1 (hypothetical protein [Cucumis melo subsp. melo])

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-78
Identity = 187/407 (45.95%), Postives = 263/407 (64.62%), Query Frame = 0

Query: 2   EEEQFLPIGC------HSAINKDVIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPSIY 61
           EEE  LPI C         ++K+ ++  +K+ ++    +  + V+    S     +PSIY
Sbjct: 6   EEETPLPISCINDHKDQFPMDKNDVVINVKDCLKELLMKRPVPVN-ESPSSEKTIKPSIY 65

Query: 62  KIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLW--RSKVNCELILD 121
           KIP+FM+ +Q+KA+EP ++SFGPYH+G +HLA ME+EK+K FQ L+    +    E I  
Sbjct: 66  KIPKFMKDIQLKAYEPYLVSFGPYHHGVEHLAPMEKEKQKVFQHLVKGDNNAATYESIAS 125

Query: 122 NVCKILEDLYGAYDNLDEKWKKD---ATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQK 181
            V  ILEDLY AYDNLDEKW+KD   + KF+E+MI+D CF+L FF +      SL T++ 
Sbjct: 126 EVSNILEDLYAAYDNLDEKWRKDVVASAKFMEMMIIDACFILVFF-SKDKSYKSLMTLRS 185

Query: 182 HIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHIL 241
            IKRD+LLLENQLPFQLL+ LY  ILP +D+++ L SL+C+   F+ +D +  +   HIL
Sbjct: 186 DIKRDILLLENQLPFQLLQLLY-KILPIKDQNKSLTSLICK-LWFVKKDELTVKGGKHIL 245

Query: 242 DMYRDSLLNP-PI----RSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKK 301
           +MYR  LL+P PI    R   +K+      +  + D+  L+ QIIPQA  L  AGIKF++
Sbjct: 246 EMYRMLLLDPIPIVLSERDESQKKAEGTKGNKKEKDESVLNSQIIPQATLLHDAGIKFRR 305

Query: 302 SQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGD-ESTVISFVTLITNL 361
           S+T SL D+ F  K GVLE+P+L V++DTET LLNVMAFEKL G+  S V SFV L+ NL
Sbjct: 306 SETESLIDIGF--KNGVLELPHLTVDDDTETKLLNVMAFEKLHGNVRSYVTSFVVLMNNL 365

Query: 362 IQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISE 392
           I  +KDV +L+ N I+ N L N +   +LF +L KG A +L  +I++
Sbjct: 366 IDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGKGVALDLESNIAK 406

BLAST of PI0022427 vs. NCBI nr
Match: XP_031742583.1 (UPF0481 protein At3g47200 [Cucumis sativus] >KGN46410.2 hypothetical protein Csa_005022 [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 2.5e-76
Identity = 178/398 (44.72%), Postives = 258/398 (64.82%), Query Frame = 0

Query: 2   EEEQFLPIGCHSAINKD--------VIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPS 61
           E +  LPI C S  +KD        V++++  NL   K   +S+AV  +G S ++  +PS
Sbjct: 6   ENQMLLPIYCRSTDHKDQLPIMDNAVVVEVKDNL--KKLLMKSVAVEKLGSSGKT-IKPS 65

Query: 62  IYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILD 121
           IYKIP F++ +  +A+ P ++SFGPYH+G+ +LA ME+EK K F+ L+    V+ E I+ 
Sbjct: 66  IYKIPNFIKDVHKEAYMPHMVSFGPYHHGEKNLAPMEQEKLKVFRHLVDVKGVDYESIVR 125

Query: 122 NVCKILEDLYGAYDNLDEKWKKD---ATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQK 181
           NV  ILEDLYG YD+LDE W KD   + +F+++MI+DGCFML FF N      SL T++ 
Sbjct: 126 NVSNILEDLYGEYDDLDEGWWKDNAGSAEFMKMMIIDGCFMLVFFSN-DEGYKSLITLKS 185

Query: 182 HIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHIL 241
            IKRD+LLLENQLPFQLL+ LY+++   +D++  L SL+  +  F+ +D +   +  HIL
Sbjct: 186 DIKRDILLLENQLPFQLLKLLYEILPIRKDQNTTLTSLI-SNLLFVEKDELAIVEKKHIL 245

Query: 242 DMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKKSQTSS 301
            MYR SLL P   SY   + +  +  D   D+FGL  Q+IPQA  LR+AGI+F+KS+  S
Sbjct: 246 HMYRASLLYPSTLSYPNMDEIKKNNKD---DKFGLKCQLIPQATLLREAGIRFRKSENKS 305

Query: 302 LSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGDESTVISFVTLITNLIQEEKD 361
           L +V+F    GVL++P L V+++TET LLNVMAFEKL    S V SFV L+ NLI  +KD
Sbjct: 306 LENVSFEK--GVLKLPLLIVDDNTETNLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKD 365

Query: 362 VSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDH 389
           V +L+++ I+ N L N++    LF++L KG + +L  +
Sbjct: 366 VELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSN 393

BLAST of PI0022427 vs. NCBI nr
Match: XP_031742694.1 (UPF0481 protein At3g47200-like [Cucumis sativus])

HSP 1 Score: 288.5 bits (737), Expect = 9.0e-74
Identity = 181/401 (45.14%), Postives = 258/401 (64.34%), Query Frame = 0

Query: 2   EEEQFLPIGCHSAINKD--------VIIKMMKNLIEPKGTEESIAVSVVGLSLRSDTQPS 61
           E +  LPI C S  +KD        V++++  NL   K   +S+AV  +G S ++  +PS
Sbjct: 6   ENQMLLPIYCRSTDHKDQLPIMDNAVVVEVKDNL--KKLLMKSVAVEKLGSSGKT-IKPS 65

Query: 62  IYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILD 121
           IYKIP F++ +  +A+ P ++SFGPYH+G+ +LA ME+EK K F+ L+    V+ E I+ 
Sbjct: 66  IYKIPNFIKDVHKEAYMPHMVSFGPYHHGEKNLAPMEQEKLKVFRHLVDVKGVDYESIVS 125

Query: 122 NVCKILEDLYGAYDNLDEKWKKD---ATKFIEIMIMDGCFMLDFFINFCPESNSLFTMQK 181
           +V  ILEDLYGAYD+LDE W KD   + KF+++MI+DGCFML F  N      SL T++ 
Sbjct: 126 DVSNILEDLYGAYDDLDEDWWKDNAGSAKFMKMMIIDGCFMLVFLSN-DEGCRSLITLKS 185

Query: 182 HIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCRSTSFINRDRIWKRKIWHIL 241
            IKRD+LLLENQLPFQLL+ LY+++   +D++  L SL+  +  F+ +D +   +  HIL
Sbjct: 186 DIKRDILLLENQLPFQLLKLLYEILPIRKDQNTTLTSLI-SNLLFVEKDELAIVEKKHIL 245

Query: 242 DMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKKSQTSS 301
            MYR SLL PP  +Y  ++ + ++  D   D+FGL  QIIPQA  LR+ GI+F+KS+  S
Sbjct: 246 HMYRASLLYPPTLNYPIRDEIKENNKD---DEFGLKCQIIPQATLLREVGIRFQKSKNKS 305

Query: 302 LSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGD-ESTVISFVTLITNLIQEEK 361
           L +V+F    GVL +P L V+++T+T LLNVMAFEKL  +  S V SFV L+ NLI+ +K
Sbjct: 306 LENVSFEK--GVLTLPSLIVDDNTKTNLLNVMAFEKLHDEVGSQVTSFVVLMHNLIRIDK 365

Query: 362 DVSVLTSNG---ILGNGLQNSKRVVELFNLLAKGPATELND 388
           DV +L SNG   I+ N   N +  V LF+ L KG   E N+
Sbjct: 366 DVQLL-SNGNINIIANAFMNDRDAVNLFSALGKGVYMETNN 395

BLAST of PI0022427 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 145.2 bits (365), Expect = 1.1e-34
Identity = 114/417 (27.34%), Postives = 205/417 (49.16%), Query Frame = 0

Query: 29  EPKGTEESIAVSV---VGLSLRSDTQPS-----IYKIPEFMRGLQVKAFEPQVISFGPYH 88
           +P+ T E   +S+   +  +L  D   S     IY++P +++    K++ PQ +S GPYH
Sbjct: 59  KPRETREEWVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIGPYH 118

Query: 89  YGKDHLARMEREKKKAFQRLLWRSKVNCELILDNVCKILEDLYGAYDNLDEKWKKDATKF 148
           +GK HL  MER K +A   ++ R+K N E+ +D + ++ E+    Y    +   K++ +F
Sbjct: 119 HGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELEEEARACYQGPID--MKNSNEF 178

Query: 149 IEIMIMDGCFMLDFF---------INFCPESNSLFT---MQKHIKRDMLLLENQLPFQLL 208
            E++++DGCF+L+ F         I +   ++ +F    +   I+RDM++LENQLP  +L
Sbjct: 179 TEMLVLDGCFVLELFKGTIQGFQKIGYA-RNDPVFAKRGLMHSIQRDMIMLENQLPLFVL 238

Query: 209 EQLYDMI--LPNE------------DRHEPLRSLVCRSTSFIN----RDRIWKRKIWHIL 268
           ++L  +    PN+                P   ++ +S   ++     D +      H L
Sbjct: 239 DRLLGLQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSLDSQEKSDELGDNGGLHCL 298

Query: 269 DMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKKSQTSS 328
           D++  SL+     + +   Y  +D   ++  Q     Q+I    ELR AG+ F + +T  
Sbjct: 299 DVFHRSLIQSSETTNQGTPY--EDMSMVEKQQ-----QLIHCVTELRGAGVNFMRKETGQ 358

Query: 329 LSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGDES-TVISFVTLITNLIQEEK 388
           L D+ F  K G L++P L +++ T++   N++AFE+     S  + S++  + NLI   +
Sbjct: 359 LWDIEF--KNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNNITSYIIFMDNLINSSQ 418

Query: 389 DVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELNDHISETLSMAAFEFLTSLWS 407
           DVS L  +GI+ + L +   V +LFN L K    +  D     LS     + +  W+
Sbjct: 419 DVSYLHHDGIIEHWLGSDSEVADLFNRLCKEVIFDPKDGYLSQLSREVNRYYSRKWN 463

BLAST of PI0022427 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-34
Identity = 114/413 (27.60%), Postives = 207/413 (50.12%), Query Frame = 0

Query: 22  KMMKNLIEPKGTEESIAVSV---VGLSLRSDTQPS-----IYKIPEFMRGLQVKAFEPQV 81
           K+ K   +P+ T E   +S+   +  +LR D   S     IY++P++++    K++ PQ 
Sbjct: 101 KLQKQNQKPEETREEWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQENNKKSYFPQT 160

Query: 82  ISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILDNVCKILEDLYGAYDNLDEKW 141
           +S GP+H+G  HL  M+R K +A   ++ R+K + E+ +D + ++ +     Y+   +  
Sbjct: 161 VSLGPFHHGNKHLLPMDRHKWRAVNMVMARTKHDIEMYIDAMKELEDRARACYEGPID-- 220

Query: 142 KKDATKFIEIMIMDGCFMLDFFINF--------CPESNSLFTMQ---KHIKRDMLLLENQ 201
              + KF E++++DGCF+L+ F              ++ +F M+     I+RDM++LENQ
Sbjct: 221 -LSSNKFSEMLVLDGCFVLELFRGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLENQ 280

Query: 202 LPFQLLEQLYDMILPNEDRHE----------------PLRSLVCRSTSFINRDRIWK--- 261
           LP  +L +L ++ L    RH+                P    + ++   + +D+ +    
Sbjct: 281 LPLFVLNRLLEIQLGK--RHQTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIA 340

Query: 262 ---RKIWHILDMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIELRKAG 321
              +   H LD++R +LL P      R   +   +     D+    +Q+I    ELR+AG
Sbjct: 341 DKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVADK--RQQQLIHCVTELREAG 400

Query: 322 IKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGDEST-VISFVT 381
           IKF+  +T    D+ F  K G LE+P L +++ T++   N++AFE+   D S  + S++ 
Sbjct: 401 IKFRTRKTDRFWDIRF--KNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSNDITSYII 460

Query: 382 LITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATE-LNDHISE 392
            + NLI   +DV  L   GI+ + L N   V +LFN L +  A +  N ++S+
Sbjct: 461 FMDNLIDSSEDVRYLHYCGIIEHWLGNDYEVADLFNRLCQEVAFDPQNSYLSQ 504

BLAST of PI0022427 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-34
Identity = 111/395 (28.10%), Postives = 191/395 (48.35%), Query Frame = 0

Query: 54  IYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILD 113
           IY++P +++    K++ PQ +S GPYH+GK  L  M+R K +A  R+L R+    ++ +D
Sbjct: 105 IYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYID 164

Query: 114 NVCKILEDLYGAYDNLDEKWKKDATKFIEIMIMDGCFMLDFFINFC--------PESNSL 173
            + ++ E     Y+         + +FIE++++DGCF+L+ F              ++ +
Sbjct: 165 AMRELEEKARACYEG---PLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPV 224

Query: 174 FTMQ---KHIKRDMLLLENQLPFQLLEQL---------------------YDMILPNED- 233
           F M+     I+RDM++LENQLP  +L +L                     +D ++P ++ 
Sbjct: 225 FAMRGSMHSIQRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEP 284

Query: 234 ----RHEPLRSLVCRSTSFINRDRIWKRKIWHILDMYRDSLL----NPPIRSYRRKEYVD 293
                   L + + R  SF   D        H LD++R SLL     P  R  R++   +
Sbjct: 285 LTKSGQSKLENSLARDKSF---DPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRN 344

Query: 294 DDFDDLDHDQFGLDRQIIPQAIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNN 353
               D         +Q+I    EL++AGIKF++ +T    D+ F  K G LE+P L +++
Sbjct: 345 TRVADKRR------QQLIHCVTELKEAGIKFRRRKTDRFWDMQF--KNGYLEIPRLLIHD 404

Query: 354 DTETTLLNVMAFEKLMGDEST-VISFVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVV 407
            T++  LN++AFE+   D S  + S++  + NLI   +DVS L   GI+ + L +   V 
Sbjct: 405 GTKSLFLNLIAFEQCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVA 464

BLAST of PI0022427 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 141.7 bits (356), Expect = 1.3e-33
Identity = 103/353 (29.18%), Postives = 182/353 (51.56%), Query Frame = 0

Query: 54  IYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILD 113
           IY++P +++    K++ PQ++S GPYH+G  HL  MER K +A   ++ R+K + E+ +D
Sbjct: 106 IYRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYID 165

Query: 114 NVCKILEDLYGAYDNLDEKWKKDATKFIEIMIMDGCFMLDFF---------INFCPESNS 173
            + ++ E     Y         +  +FIE++++DG F+++ F         I + P ++ 
Sbjct: 166 AMKELEEKARACYQG---PINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIGYAP-NDP 225

Query: 174 LFTMQ---KHIKRDMLLLENQLPFQLLEQLYDMILP------NEDRHEPLRSLVCRSTSF 233
           +F M+   + I+RDM++LENQLP+ +L+ L  +  P      N    +P    +  +   
Sbjct: 226 VFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQPFFQPLLPT--- 285

Query: 234 INRDRIWKRKIWHILDMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQAIE 293
             R+ + +    H LD+ R  LL     S       D+D   ++       +Q+I    E
Sbjct: 286 --REVLTEEGGLHCLDVLRRGLLQSSGTS-------DEDMSMVNKQ----PQQLIHCVTE 345

Query: 294 LRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKL-MGDESTV 353
           LR AG++F + +T    D+ F  K G L++P L +++ T++  LN++AFE+  +     +
Sbjct: 346 LRNAGVEFMRKETGHFWDIEF--KNGYLKIPKLLIHDGTKSLFLNLIAFEQCHIKSSKKI 405

Query: 354 ISFVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELND 388
            S++  + NLI   +DVS L   GI+ N L +   V +LFN L K    + ND
Sbjct: 406 TSYIIFMDNLINSSEDVSYLHHYGIIENWLGSDSEVSDLFNGLGKEVIFDPND 436

BLAST of PI0022427 vs. TAIR 10
Match: AT3G50180.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 139.0 bits (349), Expect = 8.2e-33
Identity = 107/363 (29.48%), Postives = 184/363 (50.69%), Query Frame = 0

Query: 54  IYKIPEFMRGLQVKAFEPQVISFGPYHYGKDHLARMEREKKKAFQRLLWRSKVNCELILD 113
           IYK+P ++ G   K++ PQ +S GPYH+G+     ME  K +A   +L R+    E+ LD
Sbjct: 180 IYKVPHYLHGNDKKSYFPQTVSLGPYHHGRQQTQSMECHKWRAVNMVLKRTNQGIEVFLD 239

Query: 114 NVCKILEDLYGAYDNLDEKWKKDATKFIEIMIMDGCFMLDF-------FINFCPESNS-L 173
            + ++ E     Y+         + +F E++++DGCF+L+        F+    + N  +
Sbjct: 240 AMIELEEKARACYEG---SIVLSSNEFTEMLLLDGCFILELLQGVNEGFLKLGYDHNDPV 299

Query: 174 FTMQ---KHIKRDMLLLENQLPFQLLEQLYDMILPNEDRHEPLRSLVCR------STSFI 233
           F ++     I+RDM++LENQLP  +L +L + + P       L  LV R       T+  
Sbjct: 300 FAVRGSMHSIQRDMIMLENQLPLFVLNRLLE-LQPGTQNQTGLVELVVRFFIPLMPTAET 359

Query: 234 NRDRIWKRKI----WHILDMYRDSLLNPPIRSYRRKEYVDDDFDDLDHDQFGLDRQIIPQ 293
             +    R +     H LD++  SLL P  RS  +  Y       L        +++IP 
Sbjct: 360 LTENSPPRGVSNGELHCLDVFHRSLLFP--RSSGKANYSRVADKHL--------QRVIPT 419

Query: 294 AIELRKAGIKFKKSQTSSLSDVNFNSKLGVLEMPYLAVNNDTETTLLNVMAFEKLMGDES 353
             ELR AG KFK ++T    D+ F++  G LE+P L +++ T++  LN++AFE+   + S
Sbjct: 420 VTELRDAGFKFKLNKTDRFWDIKFSN--GYLEIPGLLIHDGTKSLFLNLIAFEQCHIESS 479

Query: 354 T-VISFVTLITNLIQEEKDVSVLTSNGILGNGLQNSKRVVELFNLLAKGPATELND-HIS 394
             + S++  + NLI   +D+S L   GI+ + L ++  V ++FN L +    +  D ++S
Sbjct: 480 NDITSYIIFMDNLIDSPEDISYLHHCGIIEHSLGSNSEVADMFNQLCQEVVFDTKDIYLS 526

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD533.4e-2327.62UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8978.0e-0931.53Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
E5GB495.9e-7945.95Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S4E2L75.9e-7945.95uncharacterized protein LOC103499077 OS=Cucumis melo OX=3656 GN=LOC103499077 PE=... [more]
A0A5A7TA995.0e-7046.76UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G0018... [more]
A0A6J1KVQ69.4e-6945.65UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1KYV84.7e-6845.58UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_038875622.13.3e-9251.13UPF0481 protein At3g47200-like [Benincasa hispida][more]
XP_016902479.11.2e-7845.95PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo][more]
ADN33691.11.2e-7845.95hypothetical protein [Cucumis melo subsp. melo][more]
XP_031742583.12.5e-7644.72UPF0481 protein At3g47200 [Cucumis sativus] >KGN46410.2 hypothetical protein Csa... [more]
XP_031742694.19.0e-7445.14UPF0481 protein At3g47200-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G50150.11.1e-3427.34Plant protein of unknown function (DUF247) [more]
AT3G50130.13.3e-3427.60Plant protein of unknown function (DUF247) [more]
AT3G50120.13.3e-3428.10Plant protein of unknown function (DUF247) [more]
AT3G50160.11.3e-3329.18Plant protein of unknown function (DUF247) [more]
AT3G50180.18.2e-3329.48Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 54..387
e-value: 3.7E-74
score: 250.4
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 47..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0022427.1PI0022427.1mRNA