PI0022300 (gene) Melon (PI 482460) v1

Overview
NamePI0022300
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGFO_IDH_MocA domain-containing protein
Locationchr03: 8237317 .. 8240936 (-)
RNA-Seq ExpressionPI0022300
SyntenyPI0022300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTCCTAAAACCTTTAAGGCTCAAAGATGGGCGACTCAATGGTTCGTTTTGGAATTATAGGAGCAGCGAAGATTGCTCAGAAAGTTTCTCGAGCTATTGCTCTTGCTCCCAACGCCACTCTTTTGGCAATTGGTAGTCGTTCAATTGAAAAAGCCTCAAAATTTGCTTCAGATAATGGGTTGAGTCCTAAGGTCAAAATCTATAGTTCTTATAAAGCTGTGCTTGATGATCCTGATGTTGACGTTGCCTACATTCCCCTTCCAACTAGTCTTCACTTGCGTTGGGCCGTGCTCGCTGCCAAGAAGAAGAAGCATGTGCTCCTCGAGAAGCCCGTAGCTCTTAATGTAGCTGAGTTTGATCAGATTTTGGAAGCTTGTAATGCCAATGGAGTTCAATTTATGGATGGTACCATGTGGATGCACAATCCTCGTACTGCCAAGATGAAGGAGTTTCTTTGTGATGTAAAAAAGTTTGGTCAACTCAATTCGGTATGTCATTTTAATTCAATTCACAATCATGTAATTAATCTTCGTGTTAGGGAGAGCATTTTTAACGTTCATGAGTTAAACTTTTAATCTTGTATTTATATACCACTATTTATTAGGGTAATTTACATAAACATAACAAAATTCCAAAATATTTACGGCCCTTATAACAAAATAAAAAGCCCATGAAGCCGACAATATTTTTAAAATATTTCTGGTTTGCCCTTGCCGCTTTTAAATTTTGTGTGACTTCGATATCTTTCTTCTTTTTCGTTATATTTCTTCCTTCTTCCTTTTCATCTTCTTCTGCAATTTTTCTTTTATATTTTTTTGAAATCATGATCATTGTTCTATAATTATTATTATTCTTTTTCATTTTTAAAAATAAAAAATTTTAAAACACATTTTGCTTTTATTTTAAGAATTTTTTTATCAAAAGTATTTAAATAAAAATGAATTTTTTAAAAACTTTTTTTAAGTCATTTGAAATAAACTTTTAGTTTAATTATAATAATATTTTTTAAAATACACTTTCTGCTTTTTTTTTTTCCTTAGCAACATTCTCTTGGAAGATAATGTTTGACCAATCAATGATTTGGACTATTATTCGATAGTAAACGATATTACTTTCTATTTTATGTACATGTTTGTGTTGGTTTGAGTTGGGTTTAAATTTTATGAACATGTTCTCTAATTTTGTATTCTAAAAATTTGGTTAACCACATTCCCACATTTTTGTGTTGTTTTCTTATCTAAAGTAATTCATCCAGAATTTATAGAGATATATCTTTTACATAATTTAAAACACAATATTATAACAAATACAAAAATTATTTTATCTACTCTGTTAAACTTGTTAGTAGTCATATATGGAAATTGTAGTTAAATTTATTAAATGAATAACCTAATTAATAAGATTTAATAAAATCACATATCAATTTCTATAAAAGATGTTATAATATAAAATTGTAGAAGACATGTATCCCAACTAGTAATAAACTAGTTTGTATAAATTAGTGGAAGATACAATTCGAACTACACTTTCAAAAAATGGTATTTCATGTACCTAATATATGTATGCCAAATAATAGTGAAAAAAAAAACATGATATAACACAGACCATAATAATCTATACTTCAAACACAAATTATAATAACCCAATAACCCATAGACTATAATAACCATAAATTATAATAGCGCACTCTGCGTCCCAAACGTCCCCTTAAAGATTCACCTCCATATCCTCTCTTACACATTAAAAAATGTAAAAAATTGCTTGTAACATTAGAAATAAAAATGTACCTTTGTGTCTAATAAATAATTTTTTTATTGAACATTTTTCTTGAAATTAATGTAACTACAAAATTAAAGGTTTTAAATTAAAAATAAATAAAATTTATGTCTAATAAATTAGTTAATATTTTCTTTAGAAAAAATAATAATTATGAAATTTATTGGACATAAAGATTGAATTTTATTTATTTATTTTAACCAAAAAAAAGTTATTTGGTTAGCATGATAGTGTTTGTATATCTTTTACTCAAGGGGAGTGGGACCCTTGGAATAAGTACCAAAGAAAGAAACAAACATTTAAATGCATTTGTTTTTCCAAACCTATGCACCCATCCAACCACATTTGGTCATGATTGATAACTTTTTCTCGCTTATTTGAAAAATATTTTTTTAACTTTTTCTATCGCAGAGTGTTCTACGTATAAAGTTTATACGAATATAGATATGATCTATGATTTATTGTTCAATTACGAAATATATAATGTACAAAATTTGAGATAGTCAAGAAAGTAGTAAGTAGAGAAAGAATCTCATACCTCTTGAAAAGTGAAAGAAGTATTCCTTTCCTTTTTATTTAGCTTTAATTTTGACCTTCTCCCTGTTTAATCTTTTGAGATTTTCAAGGGAAACACATTATTATTGTTCTTTACTATTCCAAATTTGATGATCATAACTTTATATCGCATCCATGTTAGAAAGTTTAATATTTGGATTAAATTACAAAAATATATATTTTAAATCTTGGTTGGAGTTACTTTTAGCGAGTTGAATTGATAATTATTATAAAAGAATGTAATTATGAGAGTTGATGTGAATAACAAGGTGAGAAATATAAGTTAATATAAATAACAAGGTAATATATCTTTTCAATTGCCAGTGCACCTAAATGGTGGGTGAGTTTTTTTACCCACCCAACTCAATTACATTTCAATTGCTAAACGCTCCCTAATGTACTTGAGCCAACTAGCGGTTAGAAGTTTGGTTTGCCTTTTAGATATGGTAATTAATTGATAAATGTTTTTTTTTTTTTTTTGTTCAAATATTGTAGGTACATAGTATTTATACTTTTCGAGTTGATTCTAACTTCCTAGCGAATGATATTCGCATAAAACCTGACCTTGATGGACTTGGTGCTTTGGGTGATATTGGATGGTATTGTATTCGTTCAATCCTTTTTGCTGCTGACTTCAACTTACCACAAAGAGTGATTACCTTTCCTAATCCTGTACTGAACGAATCGGGCGTGATTTTATCTTGTGGAGGCTCTCTTTTTTGGGATGATGGAAAACTAGCAACTTTCTATTGCTCTTTCTTAGAAAACATGACTGAGAATTTGACTGTTATAGGAACAAATGGGACATTACATCTAACCGATTTCGCAATACCTTATGTAGAGAAAGAGGTATGTTTCTCCACTAATTCAAAATATGTGTTCATTGAACTTGTGACCGGTTCGAAACCTCTACCAAACAATCACACCATCCCTACCGATCTTCCCCAAGAGGCCCATATGGTGATGGAATTTTCTCATTTGGTTAAACAAATCAAAGAAAATGGCTTCAAACCTGAGGAAAAATGGCCAACCATGAGTCGAAAAACTCAACTGGTGCTCGATGCAGTGAAGGCATCATTCGATCAAGCATTCAAAGCTGTTGAAGTAGGACAATGATTCTTGTCTATGAATGTGATCACGCTTTCCTTTGATCTTATGAAATGGTCTATAAATATGATTATACTTCTATCTTTTATGTATGTTGGTTTTCAAGCTTGCATTAAGGTTGGAATGCTTCTTGAAGTTTCAAATAGTTGTGAAGGAAGCCATTCCTTGAGAATAAATGCG

mRNA sequence

CTTTTCCTAAAACCTTTAAGGCTCAAAGATGGGCGACTCAATGGTTCGTTTTGGAATTATAGGAGCAGCGAAGATTGCTCAGAAAGTTTCTCGAGCTATTGCTCTTGCTCCCAACGCCACTCTTTTGGCAATTGGTAGTCGTTCAATTGAAAAAGCCTCAAAATTTGCTTCAGATAATGGGTTGAGTCCTAAGGTCAAAATCTATAGTTCTTATAAAGCTGTGCTTGATGATCCTGATGTTGACGTTGCCTACATTCCCCTTCCAACTAGTCTTCACTTGCGTTGGGCCGTGCTCGCTGCCAAGAAGAAGAAGCATGTGCTCCTCGAGAAGCCCGTAGCTCTTAATGTAGCTGAGTTTGATCAGATTTTGGAAGCTTGTAATGCCAATGGAGTTCAATTTATGGATGGTACCATGTGGATGCACAATCCTCGTACTGCCAAGATGAAGGAGTTTCTTTGTGATGTAAAAAAGTTTGGTCAACTCAATTCGGTACATAGTATTTATACTTTTCGAGTTGATTCTAACTTCCTAGCGAATGATATTCGCATAAAACCTGACCTTGATGGACTTGGTGCTTTGGGTGATATTGGATGGTATTGTATTCGTTCAATCCTTTTTGCTGCTGACTTCAACTTACCACAAAGAGTGATTACCTTTCCTAATCCTGTACTGAACGAATCGGGCGTGATTTTATCTTGTGGAGGCTCTCTTTTTTGGGATGATGGAAAACTAGCAACTTTCTATTGCTCTTTCTTAGAAAACATGACTGAGAATTTGACTGTTATAGGAACAAATGGGACATTACATCTAACCGATTTCGCAATACCTTATGTAGAGAAAGAGGTATGTTTCTCCACTAATTCAAAATATGTGTTCATTGAACTTGTGACCGGTTCGAAACCTCTACCAAACAATCACACCATCCCTACCGATCTTCCCCAAGAGGCCCATATGGTGATGGAATTTTCTCATTTGGTTAAACAAATCAAAGAAAATGGCTTCAAACCTGAGGAAAAATGGCCAACCATGAGTCGAAAAACTCAACTGGTGCTCGATGCAGTGAAGGCATCATTCGATCAAGCATTCAAAGCTGTTGAAGTAGGACAATGATTCTTGTCTATGAATGTGATCACGCTTTCCTTTGATCTTATGAAATGGTCTATAAATATGATTATACTTCTATCTTTTATGTATGTTGGTTTTCAAGCTTGCATTAAGGTTGGAATGCTTCTTGAAGTTTCAAATAGTTGTGAAGGAAGCCATTCCTTGAGAATAAATGCG

Coding sequence (CDS)

ATGGGCGACTCAATGGTTCGTTTTGGAATTATAGGAGCAGCGAAGATTGCTCAGAAAGTTTCTCGAGCTATTGCTCTTGCTCCCAACGCCACTCTTTTGGCAATTGGTAGTCGTTCAATTGAAAAAGCCTCAAAATTTGCTTCAGATAATGGGTTGAGTCCTAAGGTCAAAATCTATAGTTCTTATAAAGCTGTGCTTGATGATCCTGATGTTGACGTTGCCTACATTCCCCTTCCAACTAGTCTTCACTTGCGTTGGGCCGTGCTCGCTGCCAAGAAGAAGAAGCATGTGCTCCTCGAGAAGCCCGTAGCTCTTAATGTAGCTGAGTTTGATCAGATTTTGGAAGCTTGTAATGCCAATGGAGTTCAATTTATGGATGGTACCATGTGGATGCACAATCCTCGTACTGCCAAGATGAAGGAGTTTCTTTGTGATGTAAAAAAGTTTGGTCAACTCAATTCGGTACATAGTATTTATACTTTTCGAGTTGATTCTAACTTCCTAGCGAATGATATTCGCATAAAACCTGACCTTGATGGACTTGGTGCTTTGGGTGATATTGGATGGTATTGTATTCGTTCAATCCTTTTTGCTGCTGACTTCAACTTACCACAAAGAGTGATTACCTTTCCTAATCCTGTACTGAACGAATCGGGCGTGATTTTATCTTGTGGAGGCTCTCTTTTTTGGGATGATGGAAAACTAGCAACTTTCTATTGCTCTTTCTTAGAAAACATGACTGAGAATTTGACTGTTATAGGAACAAATGGGACATTACATCTAACCGATTTCGCAATACCTTATGTAGAGAAAGAGGTATGTTTCTCCACTAATTCAAAATATGTGTTCATTGAACTTGTGACCGGTTCGAAACCTCTACCAAACAATCACACCATCCCTACCGATCTTCCCCAAGAGGCCCATATGGTGATGGAATTTTCTCATTTGGTTAAACAAATCAAAGAAAATGGCTTCAAACCTGAGGAAAAATGGCCAACCATGAGTCGAAAAACTCAACTGGTGCTCGATGCAGTGAAGGCATCATTCGATCAAGCATTCAAAGCTGTTGAAGTAGGACAATGA

Protein sequence

MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYSSYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDGLGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYCSFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIPTDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVGQ
Homology
BLAST of PI0022300 vs. ExPASy Swiss-Prot
Match: Q9SZ83 (Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g09670 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.8e-122
Identity = 207/356 (58.15%), Postives = 269/356 (75.56%), Query Frame = 0

Query: 3   DSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYSSY 62
           ++ +R G++G A IA+KVSRAI LAPNAT+  + SRS+EKA  FA+ N      KI+ SY
Sbjct: 4   ETQIRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSY 63

Query: 63  KAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGV 122
           +++L+DP++D  Y+PLPTSLH+ WA+ AA+K KH+LLEKPVA+NV EFD+I++AC ANGV
Sbjct: 64  ESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGV 123

Query: 123 QFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDGLG 182
           Q MDGTMW+HNPRTA +KEFL D ++FGQL +V S ++F  D +FL NDIR+KP LDGLG
Sbjct: 124 QIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLG 183

Query: 183 ALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYCSF 242
           ALGD GWY IR+ L A +F LP+ V  FP  VLNE+GVILSCG SL W+DG+ AT YCSF
Sbjct: 184 ALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSF 243

Query: 243 LENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIPTD 302
           L N+T  +T IGT GTL + DF IPY E E  F+T++K  F +LVT     P+ HT+ T+
Sbjct: 244 LANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTE 303

Query: 303 LPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEV 359
           LPQEA MV EF+ LV +IK NG KP+  WP++SRKTQLV+DAVK S D+ ++ + +
Sbjct: 304 LPQEACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of PI0022300 vs. ExPASy Swiss-Prot
Match: P49305 (Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.9e-26
Identity = 83/263 (31.56%), Postives = 143/263 (54.37%), Query Frame = 0

Query: 5   MVRFGIIGAAKIA-QKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYSSYK 64
           +VRFGIIG A IA +KV  ++  A    ++AI SR +++A   A+  G+    + Y SY 
Sbjct: 3   LVRFGIIGTAAIAVEKVIPSMLSAEGLEVVAIASRDLDRARAAATRFGIG---RSYGSYD 62

Query: 65  AVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGVQ 124
            +L DP+++  YIPLP  LH+ WA+ AA+  KHVL EKP+AL+V E  ++++  +  G +
Sbjct: 63  EILADPEIEAVYIPLPNHLHVHWAIRAAEAGKHVLCEKPLALDVEELSRLIDCRDRTGRR 122

Query: 125 FMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYT-FRVDSNFLANDIRIKPDLDGLG 184
             +  M   +P+  ++ + +    + G++ ++  ++T   +D   + ND  I     G G
Sbjct: 123 IQEAVMIRAHPQWDEIFDIVAS-GEIGEVRAIQGVFTEVNLDPKSIVNDASI-----GGG 182

Query: 185 ALGDIGWYCIRS--ILFAADFNLPQRV--ITFPNPVLNESGVILSCGGSLFWDDGKLATF 244
           AL D+G Y I +  ++FAA+   P+RV  ++  +PV    G+       L +  G+ AT 
Sbjct: 183 ALYDLGVYPIAAARMVFAAE---PERVFAVSDLDPVF---GIDRLTSAVLLFPGGRQATL 242

Query: 245 YCSFLENMTENLTVIGTNGTLHL 262
             S    +  N+ + GT  ++ L
Sbjct: 243 VVSTQLALRHNVEIFGTRKSISL 250

BLAST of PI0022300 vs. ExPASy Swiss-Prot
Match: D4GP30 (D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=HVO_B0029 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-24
Identity = 91/278 (32.73%), Postives = 129/278 (46.40%), Query Frame = 0

Query: 8   FGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYSSYKAVLD 67
           FGI+G A I  K       A      AI SR   +AS  A + G+      Y SY+A+L 
Sbjct: 3   FGILGTAGIGVKSVIPAVQASEHEAAAIASRDEARASAVADELGIP---TAYGSYEALLA 62

Query: 68  DPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGVQFMDG 127
           D  +D  YIPLP  LH  W   AA +  HVL EKP+  +  E   + + C   GV  M+ 
Sbjct: 63  DDSLDAVYIPLPNGLHADWVRAAADRGLHVLCEKPLTASADETAAVFDYCEDAGVTLMEA 122

Query: 128 TMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDGLGALGDI 187
            M+  +P T +  E +    + G + SV S ++FR+     A+DIRI PDL G G++ D+
Sbjct: 123 FMYRFHPLTERAAELV--ASELGAVVSVTSNFSFRLPDG--ADDIRIDPDLAG-GSVMDV 182

Query: 188 GWYCIRSI-LFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYCSFLENM 247
           G Y + +  LF      P RV         + GV     G L +D G  A    SF    
Sbjct: 183 GCYAVSAARLF---LGTPDRVYATTTDT-RDCGVDTRMSGVLEYDSGATARVESSFDTPE 242

Query: 248 TENLTVIGTNGTLHLTD-FAI-PYVEKEVCFSTNSKYV 283
           T+   V  T+G L     F + P    E+ ++T+ + V
Sbjct: 243 TQYYRVQTTDGRLEANPAFNVDPTAAAELTYATDGRVV 268

BLAST of PI0022300 vs. ExPASy Swiss-Prot
Match: Q9ZA33 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces violaceoruber OX=1935 GN=gra-orf26 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 82/262 (31.30%), Postives = 126/262 (48.09%), Query Frame = 0

Query: 6   VRFGIIGAAKIA-QKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYSSYKA 65
           +R G+ G A IA +++  A A +P+    A+ SRS EKA   A   G +        Y A
Sbjct: 12  LRIGVAGCADIALRRMLPAFAASPHTEPTAVASRSSEKARAAAETFGCA----AVEGYDA 71

Query: 66  VLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGVQF 125
           +L+  DVD  YIPLP +LH  W   A +  KHVL EKP+    A+  ++L+     G+  
Sbjct: 72  LLERRDVDAVYIPLPVALHAPWTERALRAGKHVLAEKPLTARAADTARLLDLARERGLVL 131

Query: 126 MDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDGLGAL 185
            +  +++H+     +++ L D    G + ++ + +T    S   A+DIR + DLDG GAL
Sbjct: 132 AENYLFVHHSAYTAVRD-LVDAGAIGDVRALSASFTIPPRS---ADDIRYRADLDG-GAL 191

Query: 186 GDIGWYCIR--SILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDD---GKLATFY 245
            DIG Y +R  S+L  ++  +   V+          GV L  GGS    D   G  A   
Sbjct: 192 LDIGVYPLRLASLLLGSELRVRGAVLRHD----TVRGVDL--GGSALLGDPGTGVSAQLV 251

Query: 246 CSFLENMTENLTVIGTNGTLHL 262
                  T    ++G+ G+L L
Sbjct: 252 FGMEHAYTAGWRLLGSEGSLTL 258

BLAST of PI0022300 vs. ExPASy Swiss-Prot
Match: Q9RR32 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces antibioticus OX=1890 GN=oleW PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.2e-19
Identity = 66/223 (29.60%), Postives = 114/223 (51.12%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIA-QKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIY 60
           M    +RFG++GAA IA ++   A+   P+ T++A+ SR   +A++FA+  G     +  
Sbjct: 1   MPSPRLRFGVLGAADIALRRTVPALLAHPDVTVVAVSSRDTARAARFAAAFG----CEAV 60

Query: 61  SSYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNA 120
             ++A+LD  D+D  Y+PLP  +H  W   A  + +HVL+EKP+    +  + ++    +
Sbjct: 61  PGHQALLDRDDIDALYVPLPVMVHTPWVEAALLRGRHVLVEKPLTATRSGAEDLIALARS 120

Query: 121 NGVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLD 180
            G+  M+    +H+ +   + + L D    G+L S+ + +T          DIR +PD+ 
Sbjct: 121 RGLVLMENFTSLHHAQHGTVTDLLRD-GTIGELRSLSAAFTIPPKPE---GDIRYQPDVG 180

Query: 181 GLGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVIL 223
           G GAL DIG Y +R+ L             F  P L+ +G +L
Sbjct: 181 G-GALLDIGIYPLRAALH------------FLGPDLHAAGAVL 202

BLAST of PI0022300 vs. ExPASy TrEMBL
Match: A0A0A0K8M9 (GFO_IDH_MocA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G052650 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.3e-174
Identity = 304/360 (84.44%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           MGDSMVRFGIIG AKIA+K+SRAIALAPNATL AIGSRSIEKAS FA DNGLS +VKIY 
Sbjct: 1   MGDSMVRFGIIGVAKIAKKISRAIALAPNATLFAIGSRSIEKASNFAFDNGLSSEVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+ VLDDPDVDV YIPLPTSLHLRWAVLAA+KKKHVL+EKPVAL+V E D ILEAC  N
Sbjct: 61  SYEVVLDDPDVDVVYIPLPTSLHLRWAVLAAEKKKHVLIEKPVALSVVELDMILEACKVN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMH+PRTAKMKEFLCD K FGQL SVHSIYTF VDS+FLANDIR KPDLDG
Sbjct: 121 GVQFMDGTMWMHSPRTAKMKEFLCDAKMFGQLKSVHSIYTFEVDSSFLANDIRTKPDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
            GALGDIGWYCIR+ILFAADF LP++VI FPNPVLNESGVILSCG SLFWDDGKLATFYC
Sbjct: 181 FGALGDIGWYCIRAILFAADFKLPKKVIAFPNPVLNESGVILSCGCSLFWDDGKLATFYC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFLE MT+N+TVIGTNGTLHLTDF IPY EKE+ F TNSKY FIE V  SKPLPN H IP
Sbjct: 241 SFLEYMTQNMTVIGTNGTLHLTDFGIPYTEKELRFYTNSKYGFIEHVASSKPLPNIHIIP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVGQ 360
           TDLPQEAHMVMEFSHLVKQIKENG KPEEKWPTMS+KTQLVLDAVKAS D+  + VEVGQ
Sbjct: 301 TDLPQEAHMVMEFSHLVKQIKENGSKPEEKWPTMSKKTQLVLDAVKASLDRGSEVVEVGQ 360

BLAST of PI0022300 vs. ExPASy TrEMBL
Match: A0A5D3BZ96 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G00490 PE=4 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.3e-174
Identity = 305/359 (84.96%), Postives = 321/359 (89.42%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           MGDSMV FGIIGAAKIAQK+SRAIAL PNATLLA+GSRSIEKA KFAS+NGLS +VKIY 
Sbjct: 1   MGDSMVHFGIIGAAKIAQKISRAIALTPNATLLAVGSRSIEKALKFASNNGLSSEVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDPD+DV YIPLPTSLHLRWAVLAA+KKKHVLLEKP ALNV E D ILEAC AN
Sbjct: 61  SYEAVLDDPDIDVIYIPLPTSLHLRWAVLAAEKKKHVLLEKPAALNVVELDMILEACKAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
            VQFMDGTMWMHNPRTAKMKEFLCD K FGQL SVHSIYTF V+SNFL +DIR KPDLDG
Sbjct: 121 EVQFMDGTMWMHNPRTAKMKEFLCDAKMFGQLKSVHSIYTFGVESNFLESDIRTKPDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
            GALGDIGWYCIRSILFAADFNLP++VI FPNPVLNESGVILSCG SLFWDDGKLATFYC
Sbjct: 181 FGALGDIGWYCIRSILFAADFNLPKKVIAFPNPVLNESGVILSCGCSLFWDDGKLATFYC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFLENMT+N+TVIGTNGTLHLTDF IPY EKEVCFSTNSKY      T S+PLPNNHTIP
Sbjct: 241 SFLENMTQNMTVIGTNGTLHLTDFVIPYTEKEVCFSTNSKYE----STSSEPLPNNHTIP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQE HMVMEFS LVKQIKENG +PEEKWP MSRKTQLVLDAVKAS DQ  + VEVG
Sbjct: 301 TDLPQEVHMVMEFSRLVKQIKENGSEPEEKWPAMSRKTQLVLDAVKASLDQGSEVVEVG 355

BLAST of PI0022300 vs. ExPASy TrEMBL
Match: A0A1S3C3U2 (uncharacterized oxidoreductase At4g09670-like OS=Cucumis melo OX=3656 GN=LOC103496561 PE=4 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.3e-174
Identity = 305/359 (84.96%), Postives = 321/359 (89.42%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           MGDSMV FGIIGAAKIAQK+SRAIAL PNATLLA+GSRSIEKA KFAS+NGLS +VKIY 
Sbjct: 1   MGDSMVHFGIIGAAKIAQKISRAIALTPNATLLAVGSRSIEKALKFASNNGLSSEVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDPD+DV YIPLPTSLHLRWAVLAA+KKKHVLLEKP ALNV E D ILEAC AN
Sbjct: 61  SYEAVLDDPDIDVIYIPLPTSLHLRWAVLAAEKKKHVLLEKPAALNVVELDMILEACKAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
            VQFMDGTMWMHNPRTAKMKEFLCD K FGQL SVHSIYTF V+SNFL +DIR KPDLDG
Sbjct: 121 EVQFMDGTMWMHNPRTAKMKEFLCDAKMFGQLKSVHSIYTFGVESNFLESDIRTKPDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
            GALGDIGWYCIRSILFAADFNLP++VI FPNPVLNESGVILSCG SLFWDDGKLATFYC
Sbjct: 181 FGALGDIGWYCIRSILFAADFNLPKKVIAFPNPVLNESGVILSCGCSLFWDDGKLATFYC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFLENMT+N+TVIGTNGTLHLTDF IPY EKEVCFSTNSKY      T S+PLPNNHTIP
Sbjct: 241 SFLENMTQNMTVIGTNGTLHLTDFVIPYTEKEVCFSTNSKYE----STSSEPLPNNHTIP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQE HMVMEFS LVKQIKENG +PEEKWP MSRKTQLVLDAVKAS DQ  + VEVG
Sbjct: 301 TDLPQEVHMVMEFSRLVKQIKENGSEPEEKWPAMSRKTQLVLDAVKASLDQGSEVVEVG 355

BLAST of PI0022300 vs. ExPASy TrEMBL
Match: A0A6J1HFT3 (uncharacterized oxidoreductase At4g09670 OS=Cucurbita moschata OX=3662 GN=LOC111463907 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.7e-162
Identity = 280/359 (77.99%), Postives = 316/359 (88.02%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           M D +VRFGIIGAA+IA+KVSRAIALAPNATL AIGSRS+EKASKFA DNG SP+VKIY 
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDP++D  Y+PLPTSLHLRWAVLAA+KKKH+LLEKPVALNV+EFD+ILEAC AN
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMHNPRTA MKEFL D ++FGQL SV+S+++F  D++FLANDIR+K DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
           LGALGD GWYCIRSILFAA+F LP+RVI FPNPVLNESGVILSCG SLFWDDGK+ATF+C
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFL N+T ++T IGTNGTLH+ DF IPY EKE  F TNSK  F ELVTG +PLP+ H +P
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQEAHMV EFSHLVK+IK NG KPE+KWP +SRKTQLVLDAVKAS D  FK VEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of PI0022300 vs. ExPASy TrEMBL
Match: A0A6J1JMT8 (uncharacterized oxidoreductase At4g09670 OS=Cucurbita maxima OX=3661 GN=LOC111487263 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.2e-162
Identity = 280/359 (77.99%), Postives = 315/359 (87.74%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           M D +VRFGIIGAA+IA+KVSRAIALAPNATL AIGSRS+EKASKFA DNG SP+VKIY 
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDP++D  Y+PLPTSLHLRWAVLAA+KKKH+LLEKPVALNV+EFD+ILEAC AN
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMHNPRTA MKEFL D ++FGQL SV+S+++F  D++FLANDIR+K DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
           LGALGD GWYCIRSILFAA F LP+RVI FPNPVLNESGVILSCG SLFWDDGK+ATF+C
Sbjct: 181 LGALGDAGWYCIRSILFAASFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFL N+T ++T IGTNGTLH+ DF IPY EKE  F TNSK  F ELVTG +PLP+ H +P
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQEAHMV EFSHLVK+IK NG KPE+KWP +SRKTQLVLDAVKAS D  FK VEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of PI0022300 vs. NCBI nr
Match: XP_004140939.1 (uncharacterized oxidoreductase At4g09670 [Cucumis sativus] >KGN46090.1 hypothetical protein Csa_005267 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 6.8e-174
Identity = 304/360 (84.44%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           MGDSMVRFGIIG AKIA+K+SRAIALAPNATL AIGSRSIEKAS FA DNGLS +VKIY 
Sbjct: 1   MGDSMVRFGIIGVAKIAKKISRAIALAPNATLFAIGSRSIEKASNFAFDNGLSSEVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+ VLDDPDVDV YIPLPTSLHLRWAVLAA+KKKHVL+EKPVAL+V E D ILEAC  N
Sbjct: 61  SYEVVLDDPDVDVVYIPLPTSLHLRWAVLAAEKKKHVLIEKPVALSVVELDMILEACKVN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMH+PRTAKMKEFLCD K FGQL SVHSIYTF VDS+FLANDIR KPDLDG
Sbjct: 121 GVQFMDGTMWMHSPRTAKMKEFLCDAKMFGQLKSVHSIYTFEVDSSFLANDIRTKPDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
            GALGDIGWYCIR+ILFAADF LP++VI FPNPVLNESGVILSCG SLFWDDGKLATFYC
Sbjct: 181 FGALGDIGWYCIRAILFAADFKLPKKVIAFPNPVLNESGVILSCGCSLFWDDGKLATFYC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFLE MT+N+TVIGTNGTLHLTDF IPY EKE+ F TNSKY FIE V  SKPLPN H IP
Sbjct: 241 SFLEYMTQNMTVIGTNGTLHLTDFGIPYTEKELRFYTNSKYGFIEHVASSKPLPNIHIIP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVGQ 360
           TDLPQEAHMVMEFSHLVKQIKENG KPEEKWPTMS+KTQLVLDAVKAS D+  + VEVGQ
Sbjct: 301 TDLPQEAHMVMEFSHLVKQIKENGSKPEEKWPTMSKKTQLVLDAVKASLDRGSEVVEVGQ 360

BLAST of PI0022300 vs. NCBI nr
Match: XP_008456688.1 (PREDICTED: uncharacterized oxidoreductase At4g09670-like [Cucumis melo] >TYK04450.1 putative oxidoreductase [Cucumis melo var. makuwa])

HSP 1 Score: 620.5 bits (1599), Expect = 8.8e-174
Identity = 305/359 (84.96%), Postives = 321/359 (89.42%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           MGDSMV FGIIGAAKIAQK+SRAIAL PNATLLA+GSRSIEKA KFAS+NGLS +VKIY 
Sbjct: 1   MGDSMVHFGIIGAAKIAQKISRAIALTPNATLLAVGSRSIEKALKFASNNGLSSEVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDPD+DV YIPLPTSLHLRWAVLAA+KKKHVLLEKP ALNV E D ILEAC AN
Sbjct: 61  SYEAVLDDPDIDVIYIPLPTSLHLRWAVLAAEKKKHVLLEKPAALNVVELDMILEACKAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
            VQFMDGTMWMHNPRTAKMKEFLCD K FGQL SVHSIYTF V+SNFL +DIR KPDLDG
Sbjct: 121 EVQFMDGTMWMHNPRTAKMKEFLCDAKMFGQLKSVHSIYTFGVESNFLESDIRTKPDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
            GALGDIGWYCIRSILFAADFNLP++VI FPNPVLNESGVILSCG SLFWDDGKLATFYC
Sbjct: 181 FGALGDIGWYCIRSILFAADFNLPKKVIAFPNPVLNESGVILSCGCSLFWDDGKLATFYC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFLENMT+N+TVIGTNGTLHLTDF IPY EKEVCFSTNSKY      T S+PLPNNHTIP
Sbjct: 241 SFLENMTQNMTVIGTNGTLHLTDFVIPYTEKEVCFSTNSKYE----STSSEPLPNNHTIP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQE HMVMEFS LVKQIKENG +PEEKWP MSRKTQLVLDAVKAS DQ  + VEVG
Sbjct: 301 TDLPQEVHMVMEFSRLVKQIKENGSEPEEKWPAMSRKTQLVLDAVKASLDQGSEVVEVG 355

BLAST of PI0022300 vs. NCBI nr
Match: XP_022963640.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >XP_022963647.1 uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >KAG7032898.1 putative oxidoreductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.0 bits (1499), Expect = 3.5e-162
Identity = 280/359 (77.99%), Postives = 316/359 (88.02%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           M D +VRFGIIGAA+IA+KVSRAIALAPNATL AIGSRS+EKASKFA DNG SP+VKIY 
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDP++D  Y+PLPTSLHLRWAVLAA+KKKH+LLEKPVALNV+EFD+ILEAC AN
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMHNPRTA MKEFL D ++FGQL SV+S+++F  D++FLANDIR+K DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
           LGALGD GWYCIRSILFAA+F LP+RVI FPNPVLNESGVILSCG SLFWDDGK+ATF+C
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFL N+T ++T IGTNGTLH+ DF IPY EKE  F TNSK  F ELVTG +PLP+ H +P
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQEAHMV EFSHLVK+IK NG KPE+KWP +SRKTQLVLDAVKAS D  FK VEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of PI0022300 vs. NCBI nr
Match: XP_022990391.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita maxima] >XP_022990392.1 uncharacterized oxidoreductase At4g09670 [Cucurbita maxima])

HSP 1 Score: 581.6 bits (1498), Expect = 4.5e-162
Identity = 280/359 (77.99%), Postives = 315/359 (87.74%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           M D +VRFGIIGAA+IA+KVSRAIALAPNATL AIGSRS+EKASKFA DNG SP+VKIY 
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDP++D  Y+PLPTSLHLRWAVLAA+KKKH+LLEKPVALNV+EFD+ILEAC AN
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMHNPRTA MKEFL D ++FGQL SV+S+++F  D++FLANDIR+K DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
           LGALGD GWYCIRSILFAA F LP+RVI FPNPVLNESGVILSCG SLFWDDGK+ATF+C
Sbjct: 181 LGALGDAGWYCIRSILFAASFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFL N+T ++T IGTNGTLH+ DF IPY EKE  F TNSK  F ELVTG +PLP+ H +P
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQEAHMV EFSHLVK+IK NG KPE+KWP +SRKTQLVLDAVKAS D  FK VEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of PI0022300 vs. NCBI nr
Match: XP_023540091.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579.7 bits (1493), Expect = 1.7e-161
Identity = 279/359 (77.72%), Postives = 314/359 (87.47%), Query Frame = 0

Query: 1   MGDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYS 60
           M D +VRFGIIGAA+IA+KVSRAIALAPNATL AIGSRS+EKASKFA DNG SP+VKIY 
Sbjct: 1   MADMIVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYG 60

Query: 61  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 120
           SY+AVLDDP++D  Y+PLPTSLHLRWAVLAA+KKKH+LLEKPVALNV+EFD+ILEAC AN
Sbjct: 61  SYEAVLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEAN 120

Query: 121 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 180
           GVQFMDGTMWMHNPRTA MKEFL D  +FGQL SV+S+++F  D++FLANDIR+K DLDG
Sbjct: 121 GVQFMDGTMWMHNPRTALMKEFLSDADQFGQLKSVNSVFSFAADADFLANDIRVKSDLDG 180

Query: 181 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 240
           LGALGD GWYCIRSILFAA+F LP+RVI FPNPVLNESGVILSCG SLFWDDGK+ATF+C
Sbjct: 181 LGALGDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHC 240

Query: 241 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 300
           SFL N+T ++T IGTNGTLH+ DF IPY EKE  F TNSK  F ELVTG +PLP+ H +P
Sbjct: 241 SFLSNLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVP 300

Query: 301 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEVG 360
           TDLPQEAHMV EFSHLVK+IK NG KPE+KWP +SRKTQLVLDAVK S D  FK VEVG
Sbjct: 301 TDLPQEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKVSLDGGFKPVEVG 359

BLAST of PI0022300 vs. TAIR 10
Match: AT4G09670.1 (Oxidoreductase family protein )

HSP 1 Score: 439.9 bits (1130), Expect = 2.0e-123
Identity = 207/356 (58.15%), Postives = 269/356 (75.56%), Query Frame = 0

Query: 3   DSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPKVKIYSSY 62
           ++ +R G++G A IA+KVSRAI LAPNAT+  + SRS+EKA  FA+ N      KI+ SY
Sbjct: 4   ETQIRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSY 63

Query: 63  KAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGV 122
           +++L+DP++D  Y+PLPTSLH+ WA+ AA+K KH+LLEKPVA+NV EFD+I++AC ANGV
Sbjct: 64  ESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGV 123

Query: 123 QFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDGLG 182
           Q MDGTMW+HNPRTA +KEFL D ++FGQL +V S ++F  D +FL NDIR+KP LDGLG
Sbjct: 124 QIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLG 183

Query: 183 ALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYCSF 242
           ALGD GWY IR+ L A +F LP+ V  FP  VLNE+GVILSCG SL W+DG+ AT YCSF
Sbjct: 184 ALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSF 243

Query: 243 LENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIPTD 302
           L N+T  +T IGT GTL + DF IPY E E  F+T++K  F +LVT     P+ HT+ T+
Sbjct: 244 LANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTE 303

Query: 303 LPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVEV 359
           LPQEA MV EF+ LV +IK NG KP+  WP++SRKTQLV+DAVK S D+ ++ + +
Sbjct: 304 LPQEACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of PI0022300 vs. TAIR 10
Match: AT1G34200.1 (Glyceraldehyde-3-phosphate dehydrogenase-like family protein )

HSP 1 Score: 381.3 bits (978), Expect = 8.4e-106
Identity = 186/356 (52.25%), Postives = 243/356 (68.26%), Query Frame = 0

Query: 2   GDSMVRFGIIGAAKIAQKVSRAIALAPNATLLAIG-SRSIEKASKFASDNGLSPKVKIYS 61
           GD+ +R G++      +K+SRAI L+PNAT+ AI  + SIE+A  FA  N   P  K++S
Sbjct: 3   GDNQIRIGLLSCTNKVRKLSRAINLSPNATISAIATTSSIEEAKSFAKSNNFPPNTKLHS 62

Query: 62  SYKAVLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNAN 121
           SY+++L+DPDVD  Y P+PT LH+ WA LAA K KH+LL+KPVALNVAEFDQI+EAC  N
Sbjct: 63  SYESLLEDPDVDAVYFPIPTRLHVEWATLAAIKGKHILLDKPVALNVAEFDQIVEACEVN 122

Query: 122 GVQFMDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDG 181
           GVQFMDGT WMH+PRT K+KEF+ D++ FGQ+ SV+S ++F    +FL +DIR+KP LDG
Sbjct: 123 GVQFMDGTQWMHSPRTDKIKEFVNDLESFGQIKSVYSCFSFAASDDFLKHDIRVKPGLDG 182

Query: 182 LGALGDIGWYCIRSILFAADFNLPQRVITFPNPVLNESGVILSCGGSLFWDDGKLATFYC 241
           LGALGD GWY I++IL   +F LP+ V      VLN++G++LSCG    W+DG  AT YC
Sbjct: 183 LGALGDAGWYTIQAILLVNNFKLPKTVTALSGSVLNDAGIVLSCGALFDWEDGVNATIYC 242

Query: 242 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 301
           SFL N+T  +T IGT G+L + DF IPY+E E  F+T+      E           H + 
Sbjct: 243 SFLANLTMEITAIGTKGSLRVHDFVIPYMETEAVFTTSGGGCVSE-----------HKVM 302

Query: 302 TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAV 357
           T+L QEA MVMEF+ LV +IK  G KP+  W   SR TQL++DAVK S D   + V
Sbjct: 303 TELSQEACMVMEFARLVGEIKNRGAKPDGFWMRFSRNTQLLVDAVKESIDNNLEPV 347

BLAST of PI0022300 vs. TAIR 10
Match: AT1G66130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.8e-69
Identity = 141/361 (39.06%), Postives = 212/361 (58.73%), Query Frame = 0

Query: 6   VRFGIIGAAKIAQKVSRAIALAPNATLLAIGSRSIEKASKFASDNGLSPK-VKIYSSYKA 65
           VRFG++G  + A K  R +  + NA ++AI   S+E A  FA+ N LSP+ V IY SY+ 
Sbjct: 7   VRFGLLGCIRFASKFVRTVTESSNAVIIAISDPSLETAKTFATGNNLSPETVTIYGSYEE 66

Query: 66  VLDDPDVDVAYIPLPTSLHLRWAVLAAKKKKHVLLEKPVALNVAEFDQILEACNANGVQF 125
           +L+D +VD  Y+ +P +   RWAV AA+KKKHVL+EKP A +  E ++I+EAC  NGVQF
Sbjct: 67  LLNDANVDAVYLTMPVTQRARWAVTAAEKKKHVLVEKPPAQDATELEKIVEACEYNGVQF 126

Query: 126 MDGTMWMHNPRTAKMKEFLCDVKKFGQLNSVHSIYTFRVDSNFLANDIRIKPDLDGL-GA 185
           MDGT+W+H+ RT K+++ + D    G +  ++S  T  V    L    R+  +  GL GA
Sbjct: 127 MDGTIWLHHQRTVKIRDTMFDSGLLGDVRHMYSTMTTPVPEQVLE---RLTKEAMGLAGA 186

Query: 186 LGDIGWYCIRSILFAADFNLPQRVITFPNPV-LNESGVILSCGGSLFWDDGKLAT--FYC 245
           +G++GWY I + L+A  + +P  V   P+ V  N  G ILSC  SL +      T   +C
Sbjct: 187 IGELGWYPIGAALWAMSYQMPISVRALPSSVSTNSVGTILSCTASLQFGSTATTTAIVHC 246

Query: 246 SFLENMTENLTVIGTNGTLHLTDFAIPYVEKEVCFSTNSKYVFIELVTGSKPLPNNHTIP 305
           SFL  ++ +L + G+ G++ + D+ IPY E +  F   S   F+++  G    P   T+ 
Sbjct: 247 SFLSQLSTDLAISGSKGSIQMNDYVIPYKEDKAWFEYTSGAKFVDMHIGWNVTPERVTVD 306

Query: 306 ---TDLPQEAHMVMEFSHLVKQIKENGFKPEEKWPTMSRKTQLVLDAVKASFDQAFKAVE 359
              T   QEA M+ EF+ LV+ IK    + + +WP +S+KTQL++DAVK S D   + V 
Sbjct: 307 CSGTPETQEAMMLREFTRLVEGIKRGDLEADRRWPDISKKTQLIVDAVKKSVDIGCEVVH 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ832.8e-12258.15Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
P493052.9e-2631.56Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1[more]
D4GP301.2e-2432.73D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / ... [more]
Q9ZA331.1e-2031.30dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces viola... [more]
Q9RR321.2e-1929.60dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces antib... [more]
Match NameE-valueIdentityDescription
A0A0A0K8M93.3e-17484.44GFO_IDH_MocA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G05265... [more]
A0A5D3BZ964.3e-17484.96Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C3U24.3e-17484.96uncharacterized oxidoreductase At4g09670-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1HFT31.7e-16277.99uncharacterized oxidoreductase At4g09670 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1JMT82.2e-16277.99uncharacterized oxidoreductase At4g09670 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
Match NameE-valueIdentityDescription
XP_004140939.16.8e-17484.44uncharacterized oxidoreductase At4g09670 [Cucumis sativus] >KGN46090.1 hypotheti... [more]
XP_008456688.18.8e-17484.96PREDICTED: uncharacterized oxidoreductase At4g09670-like [Cucumis melo] >TYK0445... [more]
XP_022963640.13.5e-16277.99uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >XP_022963647.1 un... [more]
XP_022990391.14.5e-16277.99uncharacterized oxidoreductase At4g09670 [Cucurbita maxima] >XP_022990392.1 unch... [more]
XP_023540091.11.7e-16177.72uncharacterized oxidoreductase At4g09670 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G09670.12.0e-12358.15Oxidoreductase family protein [more]
AT1G34200.18.4e-10652.25Glyceraldehyde-3-phosphate dehydrogenase-like family protein [more]
AT1G66130.14.8e-6939.06NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.360.10Dihydrodipicolinate Reductase; domain 2coord: 133..359
e-value: 2.3E-61
score: 209.3
NoneNo IPR availableGENE3D3.40.50.720coord: 2..132
e-value: 2.4E-36
score: 127.0
NoneNo IPR availablePANTHERPTHR46368:SF13SUBFAMILY NOT NAMEDcoord: 1..358
NoneNo IPR availablePANTHERPTHR46368FAMILY NOT NAMEDcoord: 1..358
NoneNo IPR availableSUPERFAMILY55347Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domaincoord: 133..318
IPR000683Gfo/Idh/MocA-like oxidoreductase, N-terminalPFAMPF01408GFO_IDH_MocAcoord: 6..125
e-value: 5.1E-20
score: 72.5
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 3..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0022300.1PI0022300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000166 nucleotide binding