PI0022033 (gene) Melon (PI 482460) v1

Overview
NamePI0022033
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionnicotianamine synthase-like
Locationchr05: 4003082 .. 4004967 (+)
RNA-Seq ExpressionPI0022033
SyntenyPI0022033
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGAAGAGCAACAACAAGAAGCACTTCTGTTGCAAAAAGTTTGTGCTCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACCACCTGCCCCAATTGGCTTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGACTCATCTCATTCAACTTTGTGCCAAAGCTGAGGCTCTCCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCGTTTCATCATCCTATTTCTAATTTGTCTGTCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTCTCGTCGTCTCCCCGATAAAGTTGCCTTTGTTGGCTCCGGTCCCCTCCCACTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTAACCCTTAGTTGTTGAATTAGTTACTAACTATATATATACTGATAGGTTGTATGGTCATGGTTGGATTTTATTATTGCTCTGATACCATGTTGAAATTACCATTGAAATTATTAGACAAACATGACGATTCATTTTAAAGTTAATTGATAATGAAATGAGTAATTCATGTATCTTCTAAACATTGTAAGATTGGTTTCTCCATCTTTTTAATATGGGATCGTCAGCATGTCCTGAAGATGTTTTTTAGTTTATCATTATTCTTGGGGTCAATTTTGTTTTGGATATTGTCTCTAAAGAATGATTTTTTTGTTCACCATTCTTGGGTCAATTTTCTTTTGGATCGAATATTTATTTGAATTTTATTGACTTTGTTACCATGTTAGAAAAATACGAAGTCCCAACTCAAACTCAATTGGTAATAATATTGGGATGAGTAACTCATGTATATTATAAGATCTCCTCCATCTTTTCAATGTAAGATTCTCGATAGAAATGCAATGTTTAGGTTTAATGGTATATTTTGTCAGTTGATTTGTAGATATTTTATTTGTAATGACAATATAGAGGTTTCAAATATATATATATATATATATATATACACACACACACACATATATGAACCATATTTTGCTAGTGTTCGAGGTGGAGCAGTTAATATTTTAACTCTACTTTTTGTTTGGTTCAAAAAGTTTTTAAACTAATAGTGGCCCACGACTTATTAGCATCAATTCTATGACTCCTTGTGAACTTCTTGCATGGTAGGTCCTACTACCAAACAAATGTATCAAACTAAACAACTCCAGATTAAACAATTTCACTCTATGTTGGATTATCGTAAATTGTTTTAATTACTTTACATCTTTTTTTTTTTTTTTTTTCCTGAATAGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACCATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTATCAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGAAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTTCATCCCACTGATGAAGTTATTAACTCCGTTGTTATTGCAAAGAAGAAAGTCGTGTTCGATCAAAGTTCAGTCGAGGACGAGGACGAGGAGGAGGAGGAGGAGAAGCGAGGTATTTTAGTTAATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATGATTGAAGAGCAACTTTCATGA

mRNA sequence

ATGGAATTTGAAGAGCAACAACAAGAAGCACTTCTGTTGCAAAAAGTTTGTGCTCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACCACCTGCCCCAATTGGCTTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGACTCATCTCATTCAACTTTGTGCCAAAGCTGAGGCTCTCCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCGTTTCATCATCCTATTTCTAATTTGTCTGTCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTCTCGTCGTCTCCCCGATAAAGTTGCCTTTGTTGGCTCCGGTCCCCTCCCACTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACCATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTATCAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGAAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTTCATCCCACTGATGAAGTTATTAACTCCGTTGTTATTGCAAAGAAGAAAGTCGTGTTCGATCAAAGTTCAGTCGAGGACGAGGACGAGGAGGAGGAGGAGGAGAAGCGAGGTATTTTAGTTAATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATGATTGAAGAGCAACTTTCATGA

Coding sequence (CDS)

ATGGAATTTGAAGAGCAACAACAAGAAGCACTTCTGTTGCAAAAAGTTTGTGCTCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACCACCTGCCCCAATTGGCTTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGACTCATCTCATTCAACTTTGTGCCAAAGCTGAGGCTCTCCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCGTTTCATCATCCTATTTCTAATTTGTCTGTCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTCTCGTCGTCTCCCCGATAAAGTTGCCTTTGTTGGCTCCGGTCCCCTCCCACTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACCATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTATCAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGAAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTTCATCCCACTGATGAAGTTATTAACTCCGTTGTTATTGCAAAGAAGAAAGTCGTGTTCGATCAAAGTTCAGTCGAGGACGAGGACGAGGAGGAGGAGGAGGAGAAGCGAGGTATTTTAGTTAATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATGATTGAAGAGCAACTTTCATGA

Protein sequence

MEFEEQQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDEEEEEEKRGILVNTSSIVLAEKCSGFNGFTPMIEEQLS
Homology
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BLAST of PI0022033 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.8e-89
Identity = 176/327 (53.82%), Postives = 242/327 (74.01%), Query Frame = 0 

Query: 8   QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMR 67
           Q+  L+ K+C LY +IS L +LKPC++VDTLF QLV TC PP P   D+  +S+ +Q MR
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNP-NIDVTKMSENIQEMR 64

Query: 68  THLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSH-SRR 127
           ++LI++C +AE  LE HFSS+L S   +P+ +L++FPYY+NYLKLS LEFD+L  + +  
Sbjct: 65  SNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGF 124

Query: 128 LPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187
           +P  VAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM FH
Sbjct: 125 VPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFH 184

Query: 188 TKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247
           T D+M+V++ LK ++VVFLAALVG++++EK +V++HL KHM+ G+ LMLRSAHG RAFLY
Sbjct: 185 TVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLY 244

Query: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDEEEEEEKRGILVN 307
           P+V+ C +E  GFE+LSV+HPTDEVINS+VI++K        V D  ++  +    +  N
Sbjct: 245 PIVEPCDLE--GFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASD----LACN 304

Query: 308 TSSI--VLAEKCSGFNGFTPMIEEQLS 332
            S I  ++ +K S    F    EEQL+
Sbjct: 305 CSKIHVIMNKKKSIIEEFAGANEEQLT 324

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BLAST of PI0022033 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.8e-87
Identity = 162/269 (60.22%), Postives = 214/269 (79.55%), Query Frame = 0 

Query: 12  LLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMRTHLI 71
           +++KVC LY QIS L +L P K+V+ LFT LV TC PP PI  D++ L Q +Q +R+HLI
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPI--DVSKLCQKIQEIRSHLI 68

Query: 72  QLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSRRLPDKV 131
           +LC +AE LLE HFS +L SS+ +P+ +L +FPY+ NY+KLSLLE++IL  ++  +P K+
Sbjct: 69  KLCGQAEGLLESHFSKIL-SSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKI 128

Query: 132 AFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVM 191
           AF+GSGPLPL+S+VLA+ HLK T FHN+DID  AN MAS LV +DPD+S RM FHT DVM
Sbjct: 129 AFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVM 188

Query: 192 EVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDI 251
           +V+  LKDY+VVFLAALVG+++E+K +V+ HL K+M+ G+ LMLRSAHGARAFLYPV+D 
Sbjct: 189 DVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLD- 248

Query: 252 CTVEASGFEILSVFHPTDEVINSVVIAKK 281
              +  GFE+LSV+HPTDEVINSV+IA+K
Sbjct: 249 -PRDLRGFEVLSVYHPTDEVINSVIIARK 272

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BLAST of PI0022033 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.9e-86
Identity = 164/283 (57.95%), Postives = 217/283 (76.68%), Query Frame = 0 

Query: 8   QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMR 67
           Q+  L+Q +C LY +IS L SLKP ++V+ LF QLV TC PP P   D+  +   +Q +R
Sbjct: 4   QDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNP-NIDVTKMCDRVQEIR 63

Query: 68  THLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSH-SRR 127
            +LI++C  AE  LE HFSS+L S   +P+ +L++FPYY+NYLKL  LEFD+L  + +  
Sbjct: 64  LNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGF 123

Query: 128 LPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187
           +P  VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM FH
Sbjct: 124 VPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFH 183

Query: 188 TKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247
           T D+M+V++ LK ++VVFLAALVG+ +EEK +V++HL KHMA G+ LMLRSAHG RAFLY
Sbjct: 184 TVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLY 243

Query: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSV 290
           P+V+ C ++  GFE+LS++HPTD+VINSVVI+KK  V    +V
Sbjct: 244 PIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKKHPVVSIGNV 283

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BLAST of PI0022033 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-84
Identity = 156/271 (57.56%), Postives = 207/271 (76.38%), Query Frame = 0 

Query: 11  LLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASL-SQPLQAMRTH 70
           L+++++  LYNQIS+L SLKP KNVDTLF QLV TC  P     D+  +  + ++ MR+H
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKVKDMRSH 66

Query: 71  LIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSRRLPD 130
           LI+LC +AE  LE HFS++L S   +P+++L +FPYY+NYLKL  LEFD+L  H+  +P 
Sbjct: 67  LIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPT 126

Query: 131 KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKD 190
           KVAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMIFHT D
Sbjct: 127 KVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTD 186

Query: 191 VMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVV 250
           V+   +GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ +MLRSAHG RAFLYP+V
Sbjct: 187 VLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIV 246

Query: 251 DICTVEASGFEILSVFHPTDEVINSVVIAKK 281
           D C  +  GFE+L+++HP+D+V+NSVVIA+K
Sbjct: 247 DSC--DLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]
BLAST of PI0022033 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-83
Identity = 157/275 (57.09%), Postives = 207/275 (75.27%), Query Frame = 0 

Query: 8   QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMR 67
           Q  L+++++  LY+QIS L SLKP KNVDTLF QLV TC  P     D+ ++ + ++ MR
Sbjct: 4   QNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVKDMR 63

Query: 68  THLIQLCAKAEALLELHFSSLLAS--SFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSR 127
            +LI+LC +AE  LE HFS++L S     +P+ +L +FPYYSNYLKL  LEFD+L  HS 
Sbjct: 64  ANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSS 123

Query: 128 RLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 187
            +P K+AFVGSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RMIF
Sbjct: 124 HVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIF 183

Query: 188 HTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 247
           HT DV+  ++GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ LMLRSAH  RAFL
Sbjct: 184 HTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFL 243

Query: 248 YPVVDICTVEASGFEILSVFHPTDEVINSVVIAKK 281
           YP+VD  + +  GF++L+++HPTD+V+NSVVIA+K
Sbjct: 244 YPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

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BLAST of PI0022033 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.0e-169
Identity = 320/338 (94.67%), Postives = 328/338 (97.04%), Query Frame = 0 

Query: 1   MEFEEQ---QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS---PPAPIGF 60
           MEFE+Q   QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS   P APIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSL 120
           D+ASLSQPLQAMR HLIQLCAKAEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLP+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKVVFDQSSVEDEDE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300

Query: 301 -EEEEEKRGILVNTSSIVLAEKCSGFNGFTPMIEEQLS 332
            EEEEEK GILV+TSSIVLAEKCSGFNGFTP+IEEQLS
Sbjct: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

[Close]
BLAST of PI0022033 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.0e-166
Identity = 312/333 (93.69%), Postives = 322/333 (96.70%), Query Frame = 0 

Query: 1   MEFEEQQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS--PPAPIGFDIAS 60
           MEF EQQQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS  PP PIGFDIAS
Sbjct: 1   MEF-EQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIAS 60

Query: 61  LSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFD 120
           LSQPL+AMR HLIQLCA+AEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSLLEFD
Sbjct: 61  LSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFD 120

Query: 121 ILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPD 180
           ILRSHSRR+PDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPD
Sbjct: 121 ILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPD 180

Query: 181 LSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSA 240
           LSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLMLRSA
Sbjct: 181 LSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSA 240

Query: 241 HGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDEEEEE 300
           HGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKV+FDQSS EDED   E+
Sbjct: 241 HGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDED---ED 300

Query: 301 EKRGILVNTSSIVLAEKCSGFNGFTPMIEEQLS 332
           EK+GILVNTSSIVLAEKCSGFNGFTPMIEE LS
Sbjct: 301 EKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]
BLAST of PI0022033 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.1e-147
Identity = 277/291 (95.19%), Postives = 283/291 (97.25%), Query Frame = 0 

Query: 1   MEFEEQ---QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS---PPAPIGF 60
           MEFE+Q   QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS   P APIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSL 120
           D+ASLSQPLQAMR HLIQLCAKAEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLP+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFD 286
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKVVFD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

[Close]
BLAST of PI0022033 vs. ExPASy TrEMBL
Match: A0A1S3BWE4 (probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 277/293 (94.54%), Postives = 283/293 (96.59%), Query Frame = 0 

Query: 1   MEFEEQ---QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS---PPAPIGF 60
           MEFE+Q   QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS   P APIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSL 120
           D+ASLSQPLQAMR HLIQLCAKAEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKG--TIFHNFDIDPTANTMASQL 180
           LEFDILRSHSRRLP+KVAFVGSGPLPLSSIVLASIHLKG  TIFHNFDIDPTANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240
           VCSDPDLSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFD 286
           LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKVVFD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

[Close]
BLAST of PI0022033 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 5.0e-129
Identity = 251/322 (77.95%), Postives = 278/322 (86.34%), Query Frame = 0 

Query: 9   EALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMRT 68
           E  LL++VCALY+QISSL+SLKP KNVDTLFTQLVLTCSPPAP G DIASL QPLQAMR 
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAP-GLDIASLPQPLQAMRA 61

Query: 69  HLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSRRLP 128
           HLI+LCA AEALLE+HFSS+LA SF +PI NLS+FPYYSNYLKLSLLEFDIL SH RR+P
Sbjct: 62  HLIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVP 121

Query: 129 DKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTK 188
             VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+FHT 
Sbjct: 122 SSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTT 181

Query: 189 DVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPV 248
           DVMEV++   +YEVVFLAALVGLEEEEKGRVL+HLGKHMA G+YLMLRSAHGARAFLYPV
Sbjct: 182 DVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPV 241

Query: 249 VDICTVEASGFEILSVFHPTDEVINSVVIAKK--KVVFDQSSVEDEDEEEEEEKRGILVN 308
           VD+  VEASGFEILSVFHPTDEVINSVVIA+K   +V D  +  D D   ++EKR +LVN
Sbjct: 242 VDVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDND--TDDEKRAVLVN 301

Query: 309 TSSIVLAEKCSGFNGFTPMIEE 329
           T  I+LAEKCSGF  F PMIEE
Sbjct: 302 T-PIILAEKCSGFKDFGPMIEE 318

[Close]
BLAST of PI0022033 vs. NCBI nr
Match: KAA0048069.1 (putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ96467.1 putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 605.9 bits (1561), Expect = 2.1e-169
Identity = 320/338 (94.67%), Postives = 328/338 (97.04%), Query Frame = 0 

Query: 1   MEFEEQ---QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS---PPAPIGF 60
           MEFE+Q   QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS   P APIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSL 120
           D+ASLSQPLQAMR HLIQLCAKAEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLP+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKVVFDQSSVEDEDE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300

Query: 301 -EEEEEKRGILVNTSSIVLAEKCSGFNGFTPMIEEQLS 332
            EEEEEK GILV+TSSIVLAEKCSGFNGFTP+IEEQLS
Sbjct: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

[Close]
BLAST of PI0022033 vs. NCBI nr
Match: XP_004144750.1 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical protein Csa_021149 [Cucumis sativus])

HSP 1 Score: 594.3 bits (1531), Expect = 6.2e-166
Identity = 312/333 (93.69%), Postives = 322/333 (96.70%), Query Frame = 0 

Query: 1   MEFEEQQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS--PPAPIGFDIAS 60
           MEF EQQQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS  PP PIGFDIAS
Sbjct: 1   MEF-EQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIAS 60

Query: 61  LSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFD 120
           LSQPL+AMR HLIQLCA+AEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSLLEFD
Sbjct: 61  LSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFD 120

Query: 121 ILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPD 180
           ILRSHSRR+PDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPD
Sbjct: 121 ILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPD 180

Query: 181 LSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSA 240
           LSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLMLRSA
Sbjct: 181 LSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSA 240

Query: 241 HGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDEEEEE 300
           HGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKV+FDQSS EDED   E+
Sbjct: 241 HGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDED---ED 300

Query: 301 EKRGILVNTSSIVLAEKCSGFNGFTPMIEEQLS 332
           EK+GILVNTSSIVLAEKCSGFNGFTPMIEE LS
Sbjct: 301 EKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]
BLAST of PI0022033 vs. NCBI nr
Match: XP_038890564.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 552.0 bits (1421), Expect = 3.5e-153
Identity = 285/332 (85.84%), Postives = 310/332 (93.37%), Query Frame = 0 

Query: 1   MEFEEQQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLS 60
           MEFE    EA LLQK+ ALY+QISSL+SL P KNVDTLFTQLVLTCSPPAP GFDIASLS
Sbjct: 1   MEFE----EAPLLQKISALYHQISSLTSLNPSKNVDTLFTQLVLTCSPPAPAGFDIASLS 60

Query: 61  QPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDIL 120
           QPLQAMR HLI+LCAKAEALLELHFSS+LASSF +PISNLS+FPYYSNYLKLSLLEFDIL
Sbjct: 61  QPLQAMRAHLIRLCAKAEALLELHFSSILASSFRNPISNLSIFPYYSNYLKLSLLEFDIL 120

Query: 121 RSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLS 180
           RSH RR+P  +AF+GSGPLPLSSIV+ASIHLKGTIFHNFD+DPTAN+MASQLVCSDPDLS
Sbjct: 121 RSHCRRVPASIAFLGSGPLPLSSIVMASIHLKGTIFHNFDLDPTANSMASQLVCSDPDLS 180

Query: 181 QRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHG 240
           QRMIFHT DVM V+ GLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAG+YLMLRSAHG
Sbjct: 181 QRMIFHTTDVMNVTNGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGAYLMLRSAHG 240

Query: 241 ARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDEEEE-EE 300
           ARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIA+KK +FD++SVEDED+++   E
Sbjct: 241 ARAFLYPVVDICDVEASGFEILSVFHPTDEVINSVVIARKKALFDRNSVEDEDDDDNAAE 300

Query: 301 KRGILVNTSSIVLAEKCSGFNGFTPMIEEQLS 332
           KRGILV+TSSIVLAEKCSGFNGF+PM+EEQLS
Sbjct: 301 KRGILVDTSSIVLAEKCSGFNGFSPMVEEQLS 328

[Close]
BLAST of PI0022033 vs. NCBI nr
Match: XP_008453482.1 (PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo])

HSP 1 Score: 530.8 bits (1366), Expect = 8.5e-147
Identity = 277/291 (95.19%), Postives = 283/291 (97.25%), Query Frame = 0 

Query: 1   MEFEEQ---QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS---PPAPIGF 60
           MEFE+Q   QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS   P APIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSL 120
           D+ASLSQPLQAMR HLIQLCAKAEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLP+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFD 286
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKVVFD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

[Close]
BLAST of PI0022033 vs. NCBI nr
Match: XP_008453473.1 (PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo])

HSP 1 Score: 525.8 bits (1353), Expect = 2.7e-145
Identity = 277/293 (94.54%), Postives = 283/293 (96.59%), Query Frame = 0 

Query: 1   MEFEEQ---QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS---PPAPIGF 60
           MEFE+Q   QQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCS   P APIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRTHLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSL 120
           D+ASLSQPLQAMR HLIQLCAKAEALLELHFSSLLASSFHHPISNLS+FPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPDKVAFVGSGPLPLSSIVLASIHLKG--TIFHNFDIDPTANTMASQL 180
           LEFDILRSHSRRLP+KVAFVGSGPLPLSSIVLASIHLKG  TIFHNFDIDPTANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240
           VCSDPDLSQRMIFHTKDVMEV+KGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFD 286
           LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIA+KKVVFD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

[Close]
BLAST of PI0022033 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 329.7 bits (844), Expect = 2.7e-90
Identity = 176/327 (53.82%), Postives = 242/327 (74.01%), Query Frame = 0 

Query: 8   QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMR 67
           Q+  L+ K+C LY +IS L +LKPC++VDTLF QLV TC PP P   D+  +S+ +Q MR
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNP-NIDVTKMSENIQEMR 64

Query: 68  THLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSH-SRR 127
           ++LI++C +AE  LE HFSS+L S   +P+ +L++FPYY+NYLKLS LEFD+L  + +  
Sbjct: 65  SNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGF 124

Query: 128 LPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187
           +P  VAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM FH
Sbjct: 125 VPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFH 184

Query: 188 TKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247
           T D+M+V++ LK ++VVFLAALVG++++EK +V++HL KHM+ G+ LMLRSAHG RAFLY
Sbjct: 185 TVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLY 244

Query: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSVEDEDEEEEEEKRGILVN 307
           P+V+ C +E  GFE+LSV+HPTDEVINS+VI++K        V D  ++  +    +  N
Sbjct: 245 PIVEPCDLE--GFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASD----LACN 304

Query: 308 TSSI--VLAEKCSGFNGFTPMIEEQLS 332
            S I  ++ +K S    F    EEQL+
Sbjct: 305 CSKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]
BLAST of PI0022033 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 319.7 bits (818), Expect = 2.8e-87
Identity = 164/283 (57.95%), Postives = 217/283 (76.68%), Query Frame = 0 

Query: 8   QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMR 67
           Q+  L+Q +C LY +IS L SLKP ++V+ LF QLV TC PP P   D+  +   +Q +R
Sbjct: 4   QDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNP-NIDVTKMCDRVQEIR 63

Query: 68  THLIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSH-SRR 127
            +LI++C  AE  LE HFSS+L S   +P+ +L++FPYY+NYLKL  LEFD+L  + +  
Sbjct: 64  LNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGF 123

Query: 128 LPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187
           +P  VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM FH
Sbjct: 124 VPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFH 183

Query: 188 TKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247
           T D+M+V++ LK ++VVFLAALVG+ +EEK +V++HL KHMA G+ LMLRSAHG RAFLY
Sbjct: 184 TVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLY 243

Query: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIAKKKVVFDQSSV 290
           P+V+ C ++  GFE+LS++HPTD+VINSVVI+KK  V    +V
Sbjct: 244 PIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKKHPVVSIGNV 283

[Close]
BLAST of PI0022033 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-85
Identity = 156/271 (57.56%), Postives = 207/271 (76.38%), Query Frame = 0 

Query: 11  LLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASL-SQPLQAMRTH 70
           L+++++  LYNQIS+L SLKP KNVDTLF QLV TC  P     D+  +  + ++ MR+H
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKVKDMRSH 66

Query: 71  LIQLCAKAEALLELHFSSLLASSFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSRRLPD 130
           LI+LC +AE  LE HFS++L S   +P+++L +FPYY+NYLKL  LEFD+L  H+  +P 
Sbjct: 67  LIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPT 126

Query: 131 KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKD 190
           KVAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMIFHT D
Sbjct: 127 KVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTD 186

Query: 191 VMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVV 250
           V+   +GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ +MLRSAHG RAFLYP+V
Sbjct: 187 VLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIV 246

Query: 251 DICTVEASGFEILSVFHPTDEVINSVVIAKK 281
           D C  +  GFE+L+++HP+D+V+NSVVIA+K
Sbjct: 247 DSC--DLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]
BLAST of PI0022033 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 310.8 bits (795), Expect = 1.3e-84
Identity = 157/275 (57.09%), Postives = 207/275 (75.27%), Query Frame = 0 

Query: 8   QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSPPAPIGFDIASLSQPLQAMR 67
           Q  L+++++  LY+QIS L SLKP KNVDTLF QLV TC  P     D+ ++ + ++ MR
Sbjct: 4   QNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVKDMR 63

Query: 68  THLIQLCAKAEALLELHFSSLLAS--SFHHPISNLSVFPYYSNYLKLSLLEFDILRSHSR 127
            +LI+LC +AE  LE HFS++L S     +P+ +L +FPYYSNYLKL  LEFD+L  HS 
Sbjct: 64  ANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSS 123

Query: 128 RLPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 187
            +P K+AFVGSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RMIF
Sbjct: 124 HVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIF 183

Query: 188 HTKDVMEVSKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 247
           HT DV+  ++GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ LMLRSAH  RAFL
Sbjct: 184 HTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFL 243

Query: 248 YPVVDICTVEASGFEILSVFHPTDEVINSVVIAKK 281
           YP+VD  + +  GF++L+++HPTD+V+NSVVIA+K
Sbjct: 244 YPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]
BLAST of PI0022033 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 53.1 bits (126), Expect = 4.8e-07
Identity = 28/56 (50.00%), Postives = 39/56 (69.64%), Query Frame = 0 

Query: 227 MAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHP--TDEVINSVVIAKK 281
           M  G+ LMLRSA G R+FLY  VD C  +  GFE+L ++HP  +D  +NSV++A+K
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPC--DLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:
BLAST of PI0022033 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9C7X53.8e-8953.82Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
Q9XGI77.8e-8760.22Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
O804833.9e-8657.95Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
Q9FKT92.1e-8457.56Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
Q9FF791.8e-8357.09Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
BLAST of PI0022033 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BBH01.0e-16994.67Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0LGI43.0e-16693.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1[more]
A0A1S3BXI84.1e-14795.19probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S3BWE41.3e-14594.54probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1G3L45.0e-12977.95nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S... [more]
BLAST of PI0022033 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0048069.12.1e-16994.67putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ9646... [more]
XP_004144750.16.2e-16693.69probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical pro... [more]
XP_038890564.13.5e-15385.84probable nicotianamine synthase 4 [Benincasa hispida][more]
XP_008453482.18.5e-14795.19PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo][more]
XP_008453473.12.7e-14594.54PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo][more]
BLAST of PI0022033 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G56430.12.7e-9053.82nicotianamine synthase 4 [more]
AT1G09240.12.8e-8757.95nicotianamine synthase 3 [more]
AT5G56080.11.5e-8557.56nicotianamine synthase 2 [more]
AT5G04950.11.3e-8457.09nicotianamine synthase 1 [more]
AT4G26483.14.8e-0750.00nicotianamine synthases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 6..282
e-value: 5.5E-114
score: 380.2
IPR004298Nicotianamine synthasePANTHERPTHR32266NICOTIANAMINE SYNTHASE 3coord: 1..283
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 4..285
score: 53.889126
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 14..282
e-value: 3.5E-60
score: 205.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0022033.1PI0022033.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
molecular_function GO:0030410 nicotianamine synthase activity