PI0021823 (gene) Melon (PI 482460) v1

Overview
NamePI0021823
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionallene oxide synthase 3-like
Locationchr01: 99932 .. 101828 (-)
RNA-Seq ExpressionPI0021823
SyntenyPI0021823
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACACACCTAACATTAAATTAAACATATGCTTCCTAAATATCACTCATTAAATTTCTATTGCTGTCCCTATATTCTTCTAATCTCAAACTTTTCTCTCCCATCTTCCCCGTTCTTCAAAAAATTATATTAAAAAATGACTTCATCTTCTTCTTCAGAACACCCACAAATTCCCCTGCCTCTGAAACCCATCCCCGGCAGCTATGGCTTCCCCATCTTTGGACCCATCAAAGATCGCTACCATTACTTCTACACCCAAGGCAGAGAAACCTTCTTCCGATCTCGAATGGCTAAATACAATTCCACTGTGTTTCGTACCAACATGCCGCCAGGTCCCTTCATCTCTTCCAATTCCAAAGTAATCGTCCTTCTCGACGCCCTCAGTTTTCCCATCCTCTTTGACACTACTAAAGTCGAGAAACGCAACGTTCTCGATGGAACTTACATGCCCTCCCTTTCCTTCACCGGAGGAATTCGTACTTGTGCTTTCTTAGACCCATCTGAAACGGAGCACTCTGTTCTCAAACGCCATTTCCTCAAATTTCTTGCCTCTCGTCACCACCAATTCATTCCTCTCTTTCGAACCTCCATTTCCGAGATGTTTGATAAACTTGAAAAGGAATTAGAAAACAATAACGTCGCCAATTTTAACCCCGTCAGTGATTCCGCATCCTTTGATTTCATTTTCCGATTGCTTTCCGATCGTAGCCCCGATAAGAATCTCAGCTCCGAGGGACCGGGTTTAGTCGACCGATGGCTTACTATGCAGCTCGCTCCATTAGCGACTCTCGGTTTGCCTAAATTTCTCAGCTGCTTTGAAGATCTCATCATCCATACTTTCCGTTTACCGTTCGCACTCGTTAAAAGCGCTTACAGGTAAATGAATGAAAACTGAAATCAACTTCTTTCTTATATAACCAAAAACTATCTAGAAGCAGAACCACAAACTGATTAATGTTGTATTACAGGAAGCTTTACGAATCGTTTTACGAGTCGTCAGGTTCATTTCTAGACGAAGCCGAGAAACAGGGAATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTCTGGCCGGATTCAACGCATACGCTGGAATGAAAGTGTTGTTGCCGATTATGCTAAACTGGGTCGGATCTGCCGGCGAGGAGCTTCATCGGAAACTCGTCGGCGAAATCAGAGCTGCCGTGAAGGAAGACGGGGGAATAACATTCGGCGCTTTAGAGAAGATGAGTCTGCTGAAATCTGTGGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCAAAGGCGAAACAGGACATTGTGATCGAGAGCCATGATTCAGCTTTCAAGATCAAGAAAGGGGAAATGATTTTTGGGTATCAGCCGATTGCGACGAAAGATCCGAAGGTGTTTGAGAATGCGGAGGAGTTTGTGGGCGATAGGTTCGTCGGTGAGAAAGGGGAGAAGCTTTTGAAGTATGTTTATTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGCGAGGGATCTGGTGGTGCTGATGTCCAGAGTAGTATTGGTGGAACTTTTCCTCCGTTATGACACGTTGGTCGTGGAGGGAACCCGGTCGTCGTTGGGTTGGTCTGTTAAGGTTAAATCGTTGACTAAAGCCTAATCGGTATCAACGGTTGCACCAGGTTTCTGTAAACTTGGCAGCATTGGATTGCTTGTATGGGTTGAAAACAAATAAAGATTAAAATTCAATTTTTAGTCCTAAGTACTCTACTAAATTTTAAATTATGATCTTATTACCAATTTATTGTTGATTTTTCTTTTTCTAAAAAAACACTCTATATTGCATTGTAATTATAATTTTTAGTAATATATCATGCATTATGTTTGCTTA

mRNA sequence

CACACACCTAACATTAAATTAAACATATGCTTCCTAAATATCACTCATTAAATTTCTATTGCTGTCCCTATATTCTTCTAATCTCAAACTTTTCTCTCCCATCTTCCCCGTTCTTCAAAAAATTATATTAAAAAATGACTTCATCTTCTTCTTCAGAACACCCACAAATTCCCCTGCCTCTGAAACCCATCCCCGGCAGCTATGGCTTCCCCATCTTTGGACCCATCAAAGATCGCTACCATTACTTCTACACCCAAGGCAGAGAAACCTTCTTCCGATCTCGAATGGCTAAATACAATTCCACTGTGTTTCGTACCAACATGCCGCCAGGTCCCTTCATCTCTTCCAATTCCAAAGTAATCGTCCTTCTCGACGCCCTCAGTTTTCCCATCCTCTTTGACACTACTAAAGTCGAGAAACGCAACGTTCTCGATGGAACTTACATGCCCTCCCTTTCCTTCACCGGAGGAATTCGTACTTGTGCTTTCTTAGACCCATCTGAAACGGAGCACTCTGTTCTCAAACGCCATTTCCTCAAATTTCTTGCCTCTCGTCACCACCAATTCATTCCTCTCTTTCGAACCTCCATTTCCGAGATGTTTGATAAACTTGAAAAGGAATTAGAAAACAATAACGTCGCCAATTTTAACCCCGTCAGTGATTCCGCATCCTTTGATTTCATTTTCCGATTGCTTTCCGATCGTAGCCCCGATAAGAATCTCAGCTCCGAGGGACCGGGTTTAGTCGACCGATGGCTTACTATGCAGCTCGCTCCATTAGCGACTCTCGGTTTGCCTAAATTTCTCAGCTGCTTTGAAGATCTCATCATCCATACTTTCCGTTTACCGTTCGCACTCGTTAAAAGCGCTTACAGGAAGCTTTACGAATCGTTTTACGAGTCGTCAGGTTCATTTCTAGACGAAGCCGAGAAACAGGGAATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTCTGGCCGGATTCAACGCATACGCTGGAATGAAAGTGTTGTTGCCGATTATGCTAAACTGGGTCGGATCTGCCGGCGAGGAGCTTCATCGGAAACTCGTCGGCGAAATCAGAGCTGCCGTGAAGGAAGACGGGGGAATAACATTCGGCGCTTTAGAGAAGATGAGTCTGCTGAAATCTGTGGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCAAAGGCGAAACAGGACATTGTGATCGAGAGCCATGATTCAGCTTTCAAGATCAAGAAAGGGGAAATGATTTTTGGGTATCAGCCGATTGCGACGAAAGATCCGAAGGTGTTTGAGAATGCGGAGGAGTTTGTGGGCGATAGGTTCGTCGGTGAGAAAGGGGAGAAGCTTTTGAAGTATGTTTATTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGCGAGGGATCTGGTGGTGCTGATGTCCAGAGTAGTATTGGTGGAACTTTTCCTCCGTTATGACACGTTGGTCGTGGAGGGAACCCGGTCGTCGTTGGGTTGGTCTGTTAAGGTTAAATCGTTGACTAAAGCCTAATCGGTATCAACGGTTGCACCAGGTTTCTGTAAACTTGGCAGCATTGGATTGCTTGTATGGGTTGAAAACAAATAAAGATTAAAATTCAATTTTTAGTCCTAAGTACTCTACTAAATTTTAAATTATGATCTTATTACCAATTTATTGTTGATTTTTCTTTTTCTAAAAAAACACTCTATATTGCATTGTAATTATAATTTTTAGTAATATATCATGCATTATGTTTGCTTA

Coding sequence (CDS)

ATGACTTCATCTTCTTCTTCAGAACACCCACAAATTCCCCTGCCTCTGAAACCCATCCCCGGCAGCTATGGCTTCCCCATCTTTGGACCCATCAAAGATCGCTACCATTACTTCTACACCCAAGGCAGAGAAACCTTCTTCCGATCTCGAATGGCTAAATACAATTCCACTGTGTTTCGTACCAACATGCCGCCAGGTCCCTTCATCTCTTCCAATTCCAAAGTAATCGTCCTTCTCGACGCCCTCAGTTTTCCCATCCTCTTTGACACTACTAAAGTCGAGAAACGCAACGTTCTCGATGGAACTTACATGCCCTCCCTTTCCTTCACCGGAGGAATTCGTACTTGTGCTTTCTTAGACCCATCTGAAACGGAGCACTCTGTTCTCAAACGCCATTTCCTCAAATTTCTTGCCTCTCGTCACCACCAATTCATTCCTCTCTTTCGAACCTCCATTTCCGAGATGTTTGATAAACTTGAAAAGGAATTAGAAAACAATAACGTCGCCAATTTTAACCCCGTCAGTGATTCCGCATCCTTTGATTTCATTTTCCGATTGCTTTCCGATCGTAGCCCCGATAAGAATCTCAGCTCCGAGGGACCGGGTTTAGTCGACCGATGGCTTACTATGCAGCTCGCTCCATTAGCGACTCTCGGTTTGCCTAAATTTCTCAGCTGCTTTGAAGATCTCATCATCCATACTTTCCGTTTACCGTTCGCACTCGTTAAAAGCGCTTACAGGAAGCTTTACGAATCGTTTTACGAGTCGTCAGGTTCATTTCTAGACGAAGCCGAGAAACAGGGAATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTCTGGCCGGATTCAACGCATACGCTGGAATGAAAGTGTTGTTGCCGATTATGCTAAACTGGGTCGGATCTGCCGGCGAGGAGCTTCATCGGAAACTCGTCGGCGAAATCAGAGCTGCCGTGAAGGAAGACGGGGGAATAACATTCGGCGCTTTAGAGAAGATGAGTCTGCTGAAATCTGTGGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCAAAGGCGAAACAGGACATTGTGATCGAGAGCCATGATTCAGCTTTCAAGATCAAGAAAGGGGAAATGATTTTTGGGTATCAGCCGATTGCGACGAAAGATCCGAAGGTGTTTGAGAATGCGGAGGAGTTTGTGGGCGATAGGTTCGTCGGTGAGAAAGGGGAGAAGCTTTTGAAGTATGTTTATTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGCGAGGGATCTGGTGGTGCTGATGTCCAGAGTAGTATTGGTGGAACTTTTCCTCCGTTATGACACGTTGGTCGTGGAGGGAACCCGGTCGTCGTTGGGTTGGTCTGTTAAGGTTAAATCGTTGACTAAAGCCTAA

Protein sequence

MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA
Homology
BLAST of PI0021823 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.4e-156
Identity = 267/468 (57.05%), Postives = 349/468 (74.57%), Query Frame = 0

Query: 14  LPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNS 73
           LP+K IPG YG P FG IKDRY + Y QG + FFRSRM KY+STVFRTN+PPGPF + NS
Sbjct: 23  LPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPPGPFNARNS 82

Query: 74  KVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKRHF 133
           KV+VL+DA+S+PILFD ++V+K N  +GT+M S SF GG + C FL  S+ +H+ LK  F
Sbjct: 83  KVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPKHTTLKGLF 142

Query: 134 LKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLSD-RSP 193
           L  L   H +FIP+F TSI+ MF  LEKEL     + FNP+ D+ SF+F+FRL  + ++P
Sbjct: 143 LSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKGTSYFNPIGDNLSFEFLFRLFCEGKNP 202

Query: 194 -DKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALVKSAYRKLYE 253
            D ++   GP +VD+W+ +QLAPL +LGL    +  EDL++HTF LP+ LVK  ++KLY 
Sbjct: 203 IDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYILVKRDHQKLYN 262

Query: 254 SFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHRK 313
           +FY S    LDEAEK G+ R++ACHN VFLAGFN+Y G+KV  P ++ W+G++G  LH +
Sbjct: 263 AFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWIGTSGPSLHAR 322

Query: 314 LVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAF 373
           LV EIR AVKE GG+T  A++KM L+KSVVYE LR+DPPVP+Q  KA+++I+I +H+S+F
Sbjct: 323 LVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKNIIITNHESSF 382

Query: 374 KIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAEN 433
            IKK E+IFGYQP+ATKD KVF+NAEEF  DRFVG  GEKLLKYVYWSNG+E + P+  +
Sbjct: 383 LIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWSNGKEIDNPSVND 442

Query: 434 KQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           KQCP +DL+VLM R+++VE F+RYDT  VE  +  LG  V  KSLTKA
Sbjct: 443 KQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKA 489

BLAST of PI0021823 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.2e-150
Identity = 263/480 (54.79%), Postives = 345/480 (71.88%), Query Frame = 0

Query: 2   TSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRT 61
           +SSSS    Q  LP + +PG YG P+ GP KDR  YFY QG+  FF+SR+ K+ STVFRT
Sbjct: 56  SSSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRT 115

Query: 62  NMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDP 121
           NMPPGPFIS N  V+VLLD  SFP+LFD +KVEK+++  GT+MPS   TGG R  ++LDP
Sbjct: 116 NMPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDP 175

Query: 122 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFD 181
           SE  H+ LK+     L+SR ++ IP F  S SE+F+ LE EL     A  N  +D A+F+
Sbjct: 176 SEPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFN 235

Query: 182 FIFRLLSDRSP-DKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 241
           F+ R L   +P D  L ++GP L+ +W+  QL PL  LGLPK L   EDL++HTFRLP A
Sbjct: 236 FLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVL---EDLVMHTFRLPPA 295

Query: 242 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 301
           LVK  Y++LY  FYE+S S LDEAEK GI+RE+ACHNL+F   FN++ G+K+  P ML W
Sbjct: 296 LVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKW 355

Query: 302 VGSAGEELHRKLVGEIRAAVKEDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAK 361
           +G AG +LH +L  EIR+ +  + G +T  A+EKM L+KSVVYE LRI+PPV  QY +AK
Sbjct: 356 IGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAK 415

Query: 362 QDIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWS 421
            D+VIESHD++F+IK+GE+++GYQP ATKDPK+F+ +EEFV DRF+GE+GEKLLK+V WS
Sbjct: 416 HDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWS 475

Query: 422 NGRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           NG ETE  +  NKQC  +D VVL+SR++LVELFLRYD+  +E   S LG ++ + SL +A
Sbjct: 476 NGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRA 532

BLAST of PI0021823 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.2e-146
Identity = 256/470 (54.47%), Postives = 334/470 (71.06%), Query Frame = 0

Query: 12  IPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTNMPPGPFISS 71
           I L  + IPG YG P  GP KDR  YFY QG+  FF SR+AKY ST+FRTNMPPGPFI+S
Sbjct: 44  IKLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITS 103

Query: 72  NSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKR 131
           N KVIVLLD  SFP+LFD +KVEK+++  GT++PS   TGG R  ++LDPSE  H  LK+
Sbjct: 104 NPKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKK 163

Query: 132 HFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLSDRS 191
                L+SR    IP F  + +E+F+ L+KE+E      FN  SD A+F+F+ R L   +
Sbjct: 164 LMFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFGVN 223

Query: 192 P-DKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALVKSAYRKLY 251
           P +  L ++GP L+ +W+ +QL P+ TLGLPKFL   +D+++HTFRLP  LVK  Y++LY
Sbjct: 224 PVETKLGTDGPALIGKWILLQLHPVITLGLPKFL---DDVLLHTFRLPPILVKKDYQRLY 283

Query: 252 ESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHR 311
           + FY +S +   EAEK GI++++ACHNL+F   FN++ GMK+  P ML  +  AG E+H 
Sbjct: 284 DFFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHT 343

Query: 312 KLVGEIRAAVKEDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDS 371
           +L  EIR+ VK  GG IT  A+EKM L+KSVVYE LR+DPPV  QY +AKQD+ IESHD+
Sbjct: 344 RLANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDA 403

Query: 372 AFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTA 431
            F++KKGE++FGYQP ATKDPK+F+   EFV DRFVGE+GEKLLK+V WSNG ETE PT 
Sbjct: 404 VFEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTV 463

Query: 432 ENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
            NKQC  +D VV++SR+ + E FLRY TL V+   S+LG S+ + SL KA
Sbjct: 464 GNKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of PI0021823 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 512.3 bits (1318), Expect = 5.9e-144
Identity = 251/468 (53.63%), Postives = 334/468 (71.37%), Query Frame = 0

Query: 14  LPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNS 73
           LP++ IPG+YG PI GPIKDR+ YFY QG E FF+SR+ KYNSTV+R NMPPG FI+ N 
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENP 111

Query: 74  KVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKRHF 133
           +V+ LLD  SFP+LFD  KVEK+++  GTYMPS   TGG R  ++LDPSE +H  LK   
Sbjct: 112 QVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLL 171

Query: 134 LKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLSDRSP- 193
              L S  ++  P F+ + SE+FD LEKEL     A+F   SD  +F+F+ R     +P 
Sbjct: 172 FFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPA 231

Query: 194 DKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALVKSAYRKLYES 253
           D  L ++ PGL+ +W+   L PL ++GLP+ +   E+ +IHTF LP ALVKS Y++LYE 
Sbjct: 232 DTKLKADAPGLITKWVLFNLHPLLSIGLPRVI---EEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 254 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHRKL 313
           F ES+G  L EA+K GI+RE+A HNL+F   FN + GMK+L P M+  +G AG ++H +L
Sbjct: 292 FLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRL 351

Query: 314 VGEIRAAVKEDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAF 373
             EIR+ +K +GG +T GA+EKM L KSVVYE LR +PPV  QY +AK+D+VIESHD+AF
Sbjct: 352 AEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAF 411

Query: 374 KIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAEN 433
           K+K GEM++GYQP+AT+DPK+F+ A+EFV +RFVGE+GEKLL++V WSNG ETE PT  N
Sbjct: 412 KVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGN 471

Query: 434 KQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           KQC  +D VVL++R+ ++E+F RYD+  +E   S LG SV   SL KA
Sbjct: 472 KQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

BLAST of PI0021823 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 499.2 bits (1284), Expect = 5.2e-140
Identity = 252/469 (53.73%), Postives = 326/469 (69.51%), Query Frame = 0

Query: 15  PLKPIPGSYGFPIFGPIKDRYHYFY-TQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNS 74
           PL+ IPGSYG P F PIKDR  YFY T GR+ +FRSRM KY STVFR NMPPGPF+SSN 
Sbjct: 7   PLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPFVSSNP 66

Query: 75  KVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKRHF 134
           KVIVLLDA SFPILFD +KVEK+++  GTYMPS   TGG R  ++LDPSE  H+ LK   
Sbjct: 67  KVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLL 126

Query: 135 LKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLSDRSPD 194
              L +  ++ IP F T+ +E+F+ LE EL  N  A FN V + A+F F+ R   + +P+
Sbjct: 127 FFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYFNSNPE 186

Query: 195 K-NLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALVKSAYRKLYES 254
           +  L +  P L+  W+   LAP   LGLP FL   ++ ++HTFRLP  L+KS Y KLY+ 
Sbjct: 187 ETKLGTSAPTLISSWVLFNLAPTLDLGLPWFL---QEPLLHTFRLPAFLIKSTYNKLYDY 246

Query: 255 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHRKL 314
           F   +   +++AEK G+ +++A HN++F   FN + G+K+L P  L W+G AGE LH +L
Sbjct: 247 FQSVATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHTQL 306

Query: 315 VGEIRAAVKE--DGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSA 374
             EIR A+K   DG +T  A+E+M L KSVVYE LRI+PPVP QY KAK +  IESHD+ 
Sbjct: 307 AEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHDAT 366

Query: 375 FKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAE 434
           F++KKGEM+FGYQP ATKDPKVF+  EE+V DRFVG+ GE LLKYV+WSNG ETE PT E
Sbjct: 367 FEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGD-GEALLKYVWWSNGPETESPTVE 426

Query: 435 NKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           NKQC  +D VVL++R+ ++ELF RYD+  +E   S LG +V +  L +A
Sbjct: 427 NKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRA 471

BLAST of PI0021823 vs. ExPASy TrEMBL
Match: A0A0A0K2U8 (Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.1e-265
Identity = 459/477 (96.23%), Postives = 467/477 (97.90%), Query Frame = 0

Query: 3   SSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTN 62
           +SSSSEHPQIPLPLKPIPGSYGFPIFGPI DRYHYFY QGRETFFRSRMAKYNSTVFRTN
Sbjct: 2   TSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTN 61

Query: 63  MPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPS 122
           MPPGPFISSNSKVIVLLDAL+FPILFDTTKVEKRNVLDGTYMPSL+FTGGIRTCAFLDPS
Sbjct: 62  MPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPS 121

Query: 123 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDF 182
           ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNP+SD ASFDF
Sbjct: 122 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDF 181

Query: 183 IFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALV 242
           IFRLLSDRSPDKN SSEGPGLVDRWLTMQLAPLATLGLPK  SCFEDLIIHTFRLPFALV
Sbjct: 182 IFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALV 241

Query: 243 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVG 302
           KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNWVG
Sbjct: 242 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVG 301

Query: 303 SAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 362
           SAGEELHRKLVGEIRAAVK DGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI
Sbjct: 302 SAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 361

Query: 363 VIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGR 422
           VIESHDSAF+IKKGEMIFGYQPIATKDPKVFENAEEFVG+RFVGEKGEKLLKYVYWSNGR
Sbjct: 362 VIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGR 421

Query: 423 ETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           ETEEPTAENKQCPARDLVVLM RVVLVELFLRYDT  VEGTRSSLGWSVKVKSLTKA
Sbjct: 422 ETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

BLAST of PI0021823 vs. ExPASy TrEMBL
Match: A0A5A7SKU9 (Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00100 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.0e-263
Identity = 455/479 (94.99%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSS EHP IPLPLKPIPG+ GFPIFGPIKDRYHYFY QGRETFFRSRMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSL+FTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFL FLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNPVSDSASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSDRSP+KN  SEGPGLVDRWLTMQLAPLATLGLPK  SCFEDLIIHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           LVKSAYRKLYESFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGEELHRKLV EIRAAVK+D G+TFGALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DIVIE+HDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDT  VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

BLAST of PI0021823 vs. ExPASy TrEMBL
Match: A0A1S3BZQ0 (allene oxide synthase 3-like OS=Cucumis melo OX=3656 GN=LOC103495194 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.0e-263
Identity = 455/479 (94.99%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSS EHP IPLPLKPIPG+ GFPIFGPIKDRYHYFY QGRETFFRSRMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSL+FTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFL FLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNPVSDSASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSDRSP+KN  SEGPGLVDRWLTMQLAPLATLGLPK  SCFEDLIIHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           LVKSAYRKLYESFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGEELHRKLV EIRAAVK+D G+TFGALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DIVIE+HDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDT  VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

BLAST of PI0021823 vs. ExPASy TrEMBL
Match: A0A6J1GLX0 (allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.2e-241
Identity = 416/478 (87.03%), Postives = 444/478 (92.89%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSSSEHP  PLPLKPIPGSYG P FGPIK RY YFY QGRE FFRSRMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISS+S+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPS+SFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFLKFLASRH +FIPLFR S+SEMF KLEKELE NN A+FN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSD  PD+ L S+G  LVDRWL MQLAPLATLGLPKFLSCFEDLIIHTF LPFA
Sbjct: 181 DFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           LVK+AYRKLY++FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAG+ELH+KL GEIRAAVKE+ G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DI+IESHDSAFKIKKGEMIFGYQPIAT+DPKVFE  EEF+GDRFVGE+GEKLLKYVYWSN
Sbjct: 361 DIIIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTK 479
           GRETEEPTAENKQCPARDLVVL+SRVVL ELFLRYDT  VEGT+SSLGWSVKVKSLTK
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

BLAST of PI0021823 vs. ExPASy TrEMBL
Match: A0A6J1IB84 (allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.2e-237
Identity = 413/479 (86.22%), Postives = 440/479 (91.86%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSSSEHP  P  LKPIPG YG P FGPIK RY YFY QGRE FFRSRMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPPSLKPIPGGYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISS+S+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPS+SFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFLKFLASRH +FIPLFRTS+SEMF KLEKELE  N A+FN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRTSVSEMFSKLEKELEKTNAADFNSVSDAASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSD  P + L S G  LVDRWL MQLAPLATLGLPKFLSCFEDLIIHTF LPFA
Sbjct: 181 DFIFRLLSDGKPGEKLGSNGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           LVK+AYRKLY++FYESS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LVKNAYRKLYDAFYESSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAG+ELHRKL GEIRAAVKE+ G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DI+IESHDSAF+IKKGEMIFGYQPIAT+DPKVFE  EEF+G+RFVGE+GEKLLKYVYWSN
Sbjct: 361 DIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGERFVGEEGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           GRETEEPTAENKQCPARDLVVL+SRVVL ELFLRYDT  VEGT+SSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFTVEGTQSSLGWSVKVKSLTKA 479

BLAST of PI0021823 vs. NCBI nr
Match: XP_004137005.1 (allene oxide synthase 3 [Cucumis sativus] >KGN43980.1 hypothetical protein Csa_017715 [Cucumis sativus])

HSP 1 Score: 923.7 bits (2386), Expect = 6.5e-265
Identity = 459/477 (96.23%), Postives = 467/477 (97.90%), Query Frame = 0

Query: 3   SSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTN 62
           +SSSSEHPQIPLPLKPIPGSYGFPIFGPI DRYHYFY QGRETFFRSRMAKYNSTVFRTN
Sbjct: 2   TSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTN 61

Query: 63  MPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPS 122
           MPPGPFISSNSKVIVLLDAL+FPILFDTTKVEKRNVLDGTYMPSL+FTGGIRTCAFLDPS
Sbjct: 62  MPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPS 121

Query: 123 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDF 182
           ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNP+SD ASFDF
Sbjct: 122 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDF 181

Query: 183 IFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALV 242
           IFRLLSDRSPDKN SSEGPGLVDRWLTMQLAPLATLGLPK  SCFEDLIIHTFRLPFALV
Sbjct: 182 IFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALV 241

Query: 243 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVG 302
           KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNWVG
Sbjct: 242 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVG 301

Query: 303 SAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 362
           SAGEELHRKLVGEIRAAVK DGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI
Sbjct: 302 SAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 361

Query: 363 VIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGR 422
           VIESHDSAF+IKKGEMIFGYQPIATKDPKVFENAEEFVG+RFVGEKGEKLLKYVYWSNGR
Sbjct: 362 VIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGR 421

Query: 423 ETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           ETEEPTAENKQCPARDLVVLM RVVLVELFLRYDT  VEGTRSSLGWSVKVKSLTKA
Sbjct: 422 ETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

BLAST of PI0021823 vs. NCBI nr
Match: XP_008454884.1 (PREDICTED: allene oxide synthase 3-like [Cucumis melo] >KAA0031258.1 allene oxide synthase 3-like [Cucumis melo var. makuwa] >TYK06709.1 allene oxide synthase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 916.4 bits (2367), Expect = 1.0e-262
Identity = 455/479 (94.99%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSS EHP IPLPLKPIPG+ GFPIFGPIKDRYHYFY QGRETFFRSRMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSL+FTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFL FLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNPVSDSASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSDRSP+KN  SEGPGLVDRWLTMQLAPLATLGLPK  SCFEDLIIHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           LVKSAYRKLYESFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGEELHRKLV EIRAAVK+D G+TFGALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DIVIE+HDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDT  VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

BLAST of PI0021823 vs. NCBI nr
Match: XP_038888826.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 884.8 bits (2285), Expect = 3.3e-253
Identity = 436/479 (91.02%), Postives = 460/479 (96.03%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSS SSE P IPLPLKPIPGSYGFPIFGPIKDRY YFY QGRE FFR+RMAKY+STVFR
Sbjct: 1   MTSSPSSEQPLIPLPLKPIPGSYGFPIFGPIKDRYDYFYIQGREKFFRTRMAKYDSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSL+FTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHS+LKRHFLKFLASRHHQFIPLFR+S+SEMF+KL++ELE N VA+FNPVSDSASF
Sbjct: 121 PSETEHSLLKRHFLKFLASRHHQFIPLFRSSVSEMFNKLDRELEKNKVADFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DF+FRLLSDRSPDK+LSS+GPGLVDRWL +QLAPLATLGLPKFLSCFED+IIHTFRLPFA
Sbjct: 181 DFVFRLLSDRSPDKSLSSKGPGLVDRWLALQLAPLATLGLPKFLSCFEDIIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           L +SAY KLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LARSAYSKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGE LHRKL  EIRAAVKEDGG+TF ALEKMSLLKSV+YEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGEGLHRKLAVEIRAAVKEDGGVTFSALEKMSLLKSVIYEVLRIDPPVPYQYAKAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DIVIESHDSAFKIKKGEMIFGYQPIATKDP+VFEN EEF+GDRFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPRVFENPEEFIGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           GRETEEPTAENKQC ARDLVVL+SRVVLVELFLRYDT  VEGT+SSLGWSVKVKSL+KA
Sbjct: 421 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTQSSLGWSVKVKSLSKA 479

BLAST of PI0021823 vs. NCBI nr
Match: XP_022952977.1 (allene oxide synthase 3-like [Cucurbita moschata])

HSP 1 Score: 842.4 bits (2175), Expect = 1.9e-240
Identity = 416/478 (87.03%), Postives = 444/478 (92.89%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSSSEHP  PLPLKPIPGSYG P FGPIK RY YFY QGRE FFRSRMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISS+S+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPS+SFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFLKFLASRH +FIPLFR S+SEMF KLEKELE NN A+FN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSD  PD+ L S+G  LVDRWL MQLAPLATLGLPKFLSCFEDLIIHTF LPFA
Sbjct: 181 DFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
           LVK+AYRKLY++FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAG+ELH+KL GEIRAAVKE+ G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DI+IESHDSAFKIKKGEMIFGYQPIAT+DPKVFE  EEF+GDRFVGE+GEKLLKYVYWSN
Sbjct: 361 DIIIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTK 479
           GRETEEPTAENKQCPARDLVVL+SRVVL ELFLRYDT  VEGT+SSLGWSVKVKSLTK
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

BLAST of PI0021823 vs. NCBI nr
Match: KAG6572010.1 (Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 842.0 bits (2174), Expect = 2.5e-240
Identity = 416/479 (86.85%), Postives = 444/479 (92.69%), Query Frame = 0

Query: 1   MTSSSSSEHPQIPLPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFR 60
           MTSSSSSEHP  PLPLKPIPGSYG P FGPIK RY YFY QGRE FFRSRMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLD 120
           TNMPPGPFISS+S+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPS+SFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASF 180
           PSETEHSVLKRHFLKFLASRH +FIPLFR S+SEMF KLEKELE NN A+FN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASF 180

Query: 181 DFIFRLLSDRSPDKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFA 240
           DFIFRLLSD  PD+ L S+G  LVDRWL MQLAPLATLGLPKFLSCFEDLIIHTF LPFA
Sbjct: 181 DFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNW 300
            VK+AYRKLY++FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 FVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAG+ELHRKL GEIRAAVKE+ G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420
           DI+IESHDSAF+IKKGEMIFGYQPIAT+DPKVFE  EEF+GDRFVGE+GEKLLKYVYWSN
Sbjct: 361 DIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           GRETEEPTAENKQCPARDLVVL+SRVVL ELFLRYDT  VEGT+SSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTKA 479

BLAST of PI0021823 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 512.3 bits (1318), Expect = 4.2e-145
Identity = 251/468 (53.63%), Postives = 334/468 (71.37%), Query Frame = 0

Query: 14  LPLKPIPGSYGFPIFGPIKDRYHYFYTQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNS 73
           LP++ IPG+YG PI GPIKDR+ YFY QG E FF+SR+ KYNSTV+R NMPPG FI+ N 
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENP 111

Query: 74  KVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKRHF 133
           +V+ LLD  SFP+LFD  KVEK+++  GTYMPS   TGG R  ++LDPSE +H  LK   
Sbjct: 112 QVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLL 171

Query: 134 LKFLASRHHQFIPLFRTSISEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLSDRSP- 193
              L S  ++  P F+ + SE+FD LEKEL     A+F   SD  +F+F+ R     +P 
Sbjct: 172 FFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPA 231

Query: 194 DKNLSSEGPGLVDRWLTMQLAPLATLGLPKFLSCFEDLIIHTFRLPFALVKSAYRKLYES 253
           D  L ++ PGL+ +W+   L PL ++GLP+ +   E+ +IHTF LP ALVKS Y++LYE 
Sbjct: 232 DTKLKADAPGLITKWVLFNLHPLLSIGLPRVI---EEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 254 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHRKL 313
           F ES+G  L EA+K GI+RE+A HNL+F   FN + GMK+L P M+  +G AG ++H +L
Sbjct: 292 FLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRL 351

Query: 314 VGEIRAAVKEDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAF 373
             EIR+ +K +GG +T GA+EKM L KSVVYE LR +PPV  QY +AK+D+VIESHD+AF
Sbjct: 352 AEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAF 411

Query: 374 KIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAEN 433
           K+K GEM++GYQP+AT+DPK+F+ A+EFV +RFVGE+GEKLL++V WSNG ETE PT  N
Sbjct: 412 KVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGN 471

Query: 434 KQCPARDLVVLMSRVVLVELFLRYDTLVVEGTRSSLGWSVKVKSLTKA 480
           KQC  +D VVL++R+ ++E+F RYD+  +E   S LG SV   SL KA
Sbjct: 472 KQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

BLAST of PI0021823 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 276.9 bits (707), Expect = 3.0e-74
Identity = 144/386 (37.31%), Postives = 230/386 (59.59%), Query Frame = 0

Query: 93  VEKRNVLDGTYMPSLSFTGGIRTCAFLDPSETEHSVLKRHFLKFLASRHHQFIPLFRTSI 152
           V+KR+VL G + PSL F GG+R   +LD +E +H+ +K   ++ L      ++   R+++
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 153 SEMFDKLEKELENNNVANFNPVSDSASFDFIFRLLS--DRSPDKNLSSEGPGLVDRWLTM 212
           +  +  +E E+  N  A++        F F+   L+  D S   +++  G   ++ WL +
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 213 QLAPLATLG-LPKFLSCFEDLIIHTFRLPFALVKSAYRKLYESFYESSGSFLDEAEKQ-G 272
           Q+ P A LG +P+ L   E++++HT+  P  L+   Y+KLY    E++G  L   +++ G
Sbjct: 124 QVIPTAKLGVVPQPL---EEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 183

Query: 273 INREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGSAGEELHRKLVGEIRAAVKEDGGITF 332
           + R++A  NL+F+ GFNAY G  V LP ++  +      L  ++  E+R        + F
Sbjct: 184 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 243

Query: 333 GALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAFKIKKGEMIFGYQPIATK 392
             + +M L+KSVVYE LR  PPVP Q+A+A++D  I SHD+ F++KKGE++ GYQP+  +
Sbjct: 244 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 303

Query: 393 DPKVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAENKQCPARDLVVLMSRVVL 452
           D  VF+  EEF  DR+VGE G +LL Y+YWSNG +T  P+A NKQC A+D+V L + +++
Sbjct: 304 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 363

Query: 453 VELFLRYDTLVVEGTRSSLGWSVKVK 475
            +LFLRYDT  + G   S+   VK K
Sbjct: 364 ADLFLRYDT--ITGDSGSIKAVVKAK 384

BLAST of PI0021823 vs. TAIR 10
Match: AT5G42590.1 (cytochrome P450, family 71, subfamily A, polypeptide 16 )

HSP 1 Score: 57.0 bits (136), Expect = 4.8e-08
Identity = 47/192 (24.48%), Postives = 85/192 (44.27%), Query Frame = 0

Query: 284 FNAYAGMKVLLPIMLNWVGS---AGEELHRKLVGEIRAAVKEDGGITFGALEKMSLLKSV 343
           F  + G       ++ W  +      E  +KL  EIR+  K +  ++   +E M+ LK+V
Sbjct: 294 FEMFLGSTTTTSAVIEWAMTRLMRNPECLKKLQDEIRSVSKMNSYVSGKEVENMNYLKAV 353

Query: 344 VYEVLRIDPPVPYQYAK-AKQDIVIESHD---------SAFKIKKGEMIFGYQPIATKDP 403
           + EVLR+ PP+P    +   +D+ ++ +D         +A+ I++    +G      +  
Sbjct: 354 IKEVLRLHPPLPLLVPRLLSEDVKLKGYDITAGTQVIINAWAIQRDTATWGSDAQEFRPE 413

Query: 404 KVFENAEEFVGDRFVGEKGEKLLKYVYWSNGRETEEPTAENKQCPARDLVVLMSRVVLVE 463
           + F++  +FVG  F         KY+ +  GR         + CP   L  +M+ V L  
Sbjct: 414 RHFDSTWDFVGRNF---------KYIPFGAGR---------RLCPGIGLGSVMASVTLAN 467

BLAST of PI0021823 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 50.8 bits (120), Expect = 3.5e-06
Identity = 53/207 (25.60%), Postives = 92/207 (44.44%), Query Frame = 0

Query: 265 EKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGS---AGEELHRKLVGEIRAAVK 324
           EK+  N  K   N +     + + G       +L W+ +      E  +KL  EIR+ ++
Sbjct: 274 EKEKNNGFKVQRNDIKFMILDMFIGGISTSSTLLEWIMTELIRNPECMKKLQNEIRSTIR 333

Query: 325 EDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAK-AKQDIVIESHDSAFKIKKGEMI 384
             G  I    +E M  LK+V+ EV R+ PP+P    +   +D+ ++ +D    I  G  +
Sbjct: 334 PHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD----IAAGTEV 393

Query: 385 FGYQPIATKDPKVF-ENAEEFVGDRFVGEKGE---KLLKYVYWSNGRETEEPTAENKQCP 444
                   +DP ++  +AEEF  +R +    +   + LKY+ + +GR         + CP
Sbjct: 394 LINAWSIHRDPAIWGPDAEEFKPERHLDSTLDYHGQDLKYIPFGSGR---------RICP 453

Query: 445 ARDLVVLMSRVVLVELFLRYDTLVVEG 463
             +L + +  V L  L  R+D  V  G
Sbjct: 454 GINLAMGLVEVTLANLVGRFDWSVDPG 467

BLAST of PI0021823 vs. TAIR 10
Match: AT1G11610.2 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 50.8 bits (120), Expect = 3.5e-06
Identity = 53/207 (25.60%), Postives = 92/207 (44.44%), Query Frame = 0

Query: 265 EKQGINREKACHNLVFLAGFNAYAGMKVLLPIMLNWVGS---AGEELHRKLVGEIRAAVK 324
           EK+  N  K   N +     + + G       +L W+ +      E  +KL  EIR+ ++
Sbjct: 274 EKEKNNGFKVQRNDIKFMILDMFIGGISTSSTLLEWIMTELIRNPECMKKLQNEIRSTIR 333

Query: 325 EDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAK-AKQDIVIESHDSAFKIKKGEMI 384
             G  I    +E M  LK+V+ EV R+ PP+P    +   +D+ ++ +D    I  G  +
Sbjct: 334 PHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD----IAAGTEV 393

Query: 385 FGYQPIATKDPKVF-ENAEEFVGDRFVGEKGE---KLLKYVYWSNGRETEEPTAENKQCP 444
                   +DP ++  +AEEF  +R +    +   + LKY+ + +GR         + CP
Sbjct: 394 LINAWSIHRDPAIWGPDAEEFKPERHLDSTLDYHGQDLKYIPFGSGR---------RICP 453

Query: 445 ARDLVVLMSRVVLVELFLRYDTLVVEG 463
             +L + +  V L  L  R+D  V  G
Sbjct: 454 GINLAMGLVEVTLANLVGRFDWSVDPG 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GZP51.4e-15657.05Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1[more]
K4BV523.2e-15054.79Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q9LLB02.2e-14654.47Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
Q962425.9e-14453.63Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q407785.2e-14053.73Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K2U83.1e-26596.23Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4... [more]
A0A5A7SKU95.0e-26394.99Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BZQ05.0e-26394.99allene oxide synthase 3-like OS=Cucumis melo OX=3656 GN=LOC103495194 PE=4 SV=1[more]
A0A6J1GLX09.2e-24187.03allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 ... [more]
A0A6J1IB841.2e-23786.22allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_004137005.16.5e-26596.23allene oxide synthase 3 [Cucumis sativus] >KGN43980.1 hypothetical protein Csa_0... [more]
XP_008454884.11.0e-26294.99PREDICTED: allene oxide synthase 3-like [Cucumis melo] >KAA0031258.1 allene oxid... [more]
XP_038888826.13.3e-25391.02allene oxide synthase 3-like [Benincasa hispida][more]
XP_022952977.11.9e-24087.03allene oxide synthase 3-like [Cucurbita moschata][more]
KAG6572010.12.5e-24086.85Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G42650.14.2e-14553.63allene oxide synthase [more]
AT4G15440.13.0e-7437.31hydroperoxide lyase 1 [more]
AT5G42590.14.8e-0824.48cytochrome P450, family 71, subfamily A, polypeptide 16 [more]
AT1G11610.13.5e-0625.60cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
AT1G11610.23.5e-0625.60cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 334..350
score: 37.1
coord: 371..385
score: 16.92
coord: 387..405
score: 27.53
IPR001128Cytochrome P450PFAMPF00067p450coord: 307..417
e-value: 3.4E-16
score: 59.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 14..479
e-value: 1.9E-113
score: 381.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 13..464
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 14..460
NoneNo IPR availablePANTHERPTHR24286:SF302HYDROPEROXIDE LYASEcoord: 14..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021823.1PI0021823.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen