PI0021513 (gene) Melon (PI 482460) v1

Overview
NamePI0021513
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGDSL esterase/lipase
Locationchr07: 7133227 .. 7135149 (-)
RNA-Seq ExpressionPI0021513
SyntenyPI0021513
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAGTCGGGTTGTTACAATTTGGTTGCTTCTTAGCAAAGCATGCAATGCTGTAGAGCCTAAAACTTCACGATTTTTTCCTGCCATTCTCGTATTTGGTGATTCTACAATTGACCCAGGTAATAACAACTACATTCTCACCTTGTTCAGAGCTAATTTCCGTCCATATGGCAGAGATTTCCCTGGCCAGATTCCCACAGGAAGGTTCTCCAATGGAAAACTCGTTCCTGATCTTCTGGCATCAAGATTAGGGATAAAGGAGTTTGTTCCTGCTTTTTTGGATCCCAAGCTATCTGACGATGACCTGAGAACCGGTGTGAGTTTTGGGTCAACAGGGTCAGGTTATGATGATCTTACTACTACTATATCTGGTGTCATTCCAGTGCTAAAGCAGATTGATCTTTTTGAGAATACATTAAAAACTAGAAAGAATTGTGGGTGAAGAGGAGGCTAAAAAGATTCTCAGTGATGGTTTGACTGTTGTAAGTGCAGGAACTAATGACTTCCTATTTAATTTCTATGACATTCCTAGAAGGTCACTGGAGTACAATATCAGTGGTTACCAAGTTTTATCTAGGTACCGCAACTGATGCAAATTTTAAGTGCAATCATATCCACGATAGTGTAAAATTTTAATAAGTTCACAGTCTACCAAAATTTGAGGGCATATCACGCATTGCATTACCAGGGCATATCTAAAGTTAACATCAATTATAAGAACTAACATTCAATTAGAATTTTATTGATGGTATGAAATTACAAGAAGGCTTAAGAAGCCGTGAGAGCTTACAAATAGCAATTCCATTGTGATATGAGAGAAGTAAAGTTGTAATCACATCAAGTATAAGCACTTATCATGCCCATTTGTTATTCATCAACTTTTCACCTTTCTAAATTATTTTCGGAGAAAATATAACTTCACGCTAATAGATAGAAGTTTCTAATTATTCTTTATGATAGACCTATCAGTAGATTTTACATCTTTCTTCAATATAAACAGAATAAACAGTCAATATAAACAAGACAAATTTACGCTTTTGGTCCTTCAAGTTTGGAATTGGTAGAATTTTGGTCCTTGAGTTTCAACCTTAACATTTTAACCCCTCAAGTTTGATTTTTTAGCCTTTTAGTACTGACTGTCGATTTTCTACTATGACGGGACCAAAATGTTACCAAAATTAAACAAGAAGGCTAAAGTGTGTAGTGAACTTTCGTTCCAGTTTACCAAGCGAAGGGAATAAAACATGTTGCACATGATAAATATTAGACTAAGCCAAAACAAAAAGTAAGTAGGAGCTTCAAGTGGTGTGTAGTTAAGCAACTAAGAATTTTTTGGGAGATTGGATAGGGATTGTATCAGCTCGGATGTCGCAACATAATTGTAGCAAGGCTTCCTCCAATTGGTTGCATACCTATTCAAGATACTCTAGCATTTCAGGATCCTAAAGATCGAAAATGTCTTCAAAATCAAAACTCAGATTCCCAAAATTACAATCACAACCTCACTAAGTTGCTGCACGATTTACAAGAACTTCTTCCTGGAAGCAAAATTCACTACATTGACATTTACAATCTGGTGATTGACATGATCAACCATCCACAAAAATATGGTAAGCGATGGAATTACGCTACCTGACCTGACCCAAAATTCCCGTTTATCATTATCATTAGTAAACTTCACCTAGTTTTGTTTGACGCATTTCATCGAACAAGTTTCATTCAAACGACAAAAGGGTGTTGCGAGACTGGTTTGGGGGAAACAGGACCTTTGGGTAACCCAAAGACACCAACATGTGAGGAGTCTAAGAAATACTTGTTTTTGGATAGTGTTCATCCTACTGAATCAGCCTACAATTTCATTTGCAACAACATTGTCAAGCAACTAGGGTCGTACCTCATTCAGAATCCATCTTCTCCCTAA

mRNA sequence

ATGAAAAGTCGGGTTGTTACAATTTGGTTGCTTCTTAGCAAAGCATGCAATGCTGTAGAGCCTAAAACTTCACGATTTTTTCCTGCCATTCTCGTATTTGGTGATTCTACAATTGACCCAGGTAATAACAACTACATTCTCACCTTGTTCAGAGCTAATTTCCGTCCATATGGCAGAGATTTCCCTGGCCAGATTCCCACAGGAAGGTTCTCCAATGGAAAACTCGTTCCTGATCTTCTGGCATCAAGATTAGGGATAAAGGAGTTTGTTCCTGCTTTTTTGGATCCCAAGCTATCTGACGATGACCTGAGAACCGGTGTGAGTTTTGGGTCAACAGGGTCAGGTTATGATGATCTTACTACTACTATATCTGAAAGAATTGTGGGTGAAGAGGAGGCTAAAAAGATTCTCAGTGATGGTTTGACTGTTGTAAGTGCAGGAACTAATGACTTCCTATTTAATTTCTATGACATTCCTAGAAGGTCACTGGAGTACAATATCAGTGGTTACCAAGGATTGTATCAGCTCGGATGTCGCAACATAATTGTAGCAAGGCTTCCTCCAATTGGTTGCATACCTATTCAAGATACTCTAGCATTTCAGGATCCTAAAGATCGAAAATGTCTTCAAAATCAAAACTCAGATTCCCAAAATTACAATCACAACCTCACTAAGTTGCTGCACGATTTACAAGAACTTCTTCCTGGAAGCAAAATTCACTACATTGACATTTACAATCTGGTGATTGACATGATCAACCATCCACAAAAATATGTTTTGTTTGACGCATTTCATCGAACAAGTTTCATTCAAACGACAAAAGGGTGTTGCGAGACTGGTTTGGGGGAAACAGGACCTTTGGGTAACCCAAAGACACCAACATGTGAGGAGTCTAAGAAATACTTGTTTTTGGATAGTGTTCATCCTACTGAATCAGCCTACAATTTCATTTGCAACAACATTGTCAAGCAACTAGGGTCGTACCTCATTCAGAATCCATCTTCTCCCTAA

Coding sequence (CDS)

ATGAAAAGTCGGGTTGTTACAATTTGGTTGCTTCTTAGCAAAGCATGCAATGCTGTAGAGCCTAAAACTTCACGATTTTTTCCTGCCATTCTCGTATTTGGTGATTCTACAATTGACCCAGGTAATAACAACTACATTCTCACCTTGTTCAGAGCTAATTTCCGTCCATATGGCAGAGATTTCCCTGGCCAGATTCCCACAGGAAGGTTCTCCAATGGAAAACTCGTTCCTGATCTTCTGGCATCAAGATTAGGGATAAAGGAGTTTGTTCCTGCTTTTTTGGATCCCAAGCTATCTGACGATGACCTGAGAACCGGTGTGAGTTTTGGGTCAACAGGGTCAGGTTATGATGATCTTACTACTACTATATCTGAAAGAATTGTGGGTGAAGAGGAGGCTAAAAAGATTCTCAGTGATGGTTTGACTGTTGTAAGTGCAGGAACTAATGACTTCCTATTTAATTTCTATGACATTCCTAGAAGGTCACTGGAGTACAATATCAGTGGTTACCAAGGATTGTATCAGCTCGGATGTCGCAACATAATTGTAGCAAGGCTTCCTCCAATTGGTTGCATACCTATTCAAGATACTCTAGCATTTCAGGATCCTAAAGATCGAAAATGTCTTCAAAATCAAAACTCAGATTCCCAAAATTACAATCACAACCTCACTAAGTTGCTGCACGATTTACAAGAACTTCTTCCTGGAAGCAAAATTCACTACATTGACATTTACAATCTGGTGATTGACATGATCAACCATCCACAAAAATATGTTTTGTTTGACGCATTTCATCGAACAAGTTTCATTCAAACGACAAAAGGGTGTTGCGAGACTGGTTTGGGGGAAACAGGACCTTTGGGTAACCCAAAGACACCAACATGTGAGGAGTCTAAGAAATACTTGTTTTTGGATAGTGTTCATCCTACTGAATCAGCCTACAATTTCATTTGCAACAACATTGTCAAGCAACTAGGGTCGTACCTCATTCAGAATCCATCTTCTCCCTAA

Protein sequence

MKSRVVTIWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTISERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNISGYQGLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQNPSSP
Homology
BLAST of PI0021513 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 8.5e-81
Identity = 160/330 (48.48%), Postives = 202/330 (61.21%), Query Frame = 0

Query: 27  FPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPTGRFSNGKLVPDLLASRLGI 86
           FPAILVFGDSTID GNNNYI T  RANF PYG +FPG   TGRFSNGKL+PD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 87  KEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDL----TTTIS---------------ERI 146
           K+ VP FLDP LSD D+ TGV F S GSGYD+L    T+T+S                +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 147 VGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNISGYQG------------LYQ 206
           VG+E+A  I+S+ L +VS+GTNDF  N YD P R  +  + GYQ             LY 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 207 LGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNHNLTKLLHDLQELLP 266
           +GCR I+V  LPP+GC+PIQ T+A Q   +R+C+  QNSDSQ +N  L   L ++Q  L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 267 GSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGLGETGPLGNPKTPTC 326
           GS I Y DIY  + DM  +PQ+Y L          +TT+GCC TG  E   L N  T  C
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGL---------KETTRGCCGTGEIELAYLCNALTRIC 334

BLAST of PI0021513 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-78
Identity = 160/357 (44.82%), Postives = 219/357 (61.34%), Query Frame = 0

Query: 3   SRVVTIWLLLS----KACNAVEPKTSR-FFPAILVFGDSTIDPGNNNYIL-TLFRANFRP 62
           S+ +T+ L ++     +C+A    T++  FPAIL+FGDST+D GNNNY L T+FRA   P
Sbjct: 4   SKAITLTLFITTTLLASCDAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFP 63

Query: 63  YGRDFPGQIPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGY 122
           YG D P     GRFSNGKL+ D++A++L IKEF+P FL P LSD D+ TGV F S G+GY
Sbjct: 64  YGMDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGY 123

Query: 123 DDLTT------TISER-------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYD 182
           DDLT+       +SE+             IVG+++A +I+++   VVSAG NDF+ N+YD
Sbjct: 124 DDLTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYD 183

Query: 183 IPRRSLEYN-ISGYQG------------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPK 242
           IP R LEY  ISGYQ             LY LG RN++V  LPP+GC+PI  T  F++  
Sbjct: 184 IPSRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-I 243

Query: 243 DRKCLQNQNSDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAF 302
            R CL++ N DS  YN  L KLL  ++  LPGSK  Y D+YN +++MI +P KY      
Sbjct: 244 FRFCLEHHNKDSVLYNEKLQKLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKY------ 303

Query: 303 HRTSFIQTTKGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNI 322
               F +T +GCC TG  ET  + N  +P C+   +++F DS+HP+E+ YN I N +
Sbjct: 304 ---GFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFMFFDSIHPSEATYNVIGNRL 350

BLAST of PI0021513 vs. ExPASy Swiss-Prot
Match: Q9SIQ3 (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.7e-78
Identity = 160/355 (45.07%), Postives = 216/355 (60.85%), Query Frame = 0

Query: 3   SRVVTIWLLLSKA----CNAVEPKTSR-FFPAILVFGDSTIDPGNNNYIL-TLFRANFRP 62
           S+ +T+ L ++      CNA    T++  FPAIL+FGDST+D GNNNY L T+FRA   P
Sbjct: 4   SKAITLTLFIATTLLAPCNAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFP 63

Query: 63  YGRDFPGQIPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGY 122
           YG D P     GRFSNGKL+ D++A++L IKEF+P FL P LSD D+ TGV F S G+GY
Sbjct: 64  YGMDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGY 123

Query: 123 DDLTT------TISER-------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYD 182
           DDLT+       +SE+             IVG+++A +I+++   VVSAG NDF+ N+Y+
Sbjct: 124 DDLTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYE 183

Query: 183 IPRRSLEYN-ISGYQG------------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPK 242
           IP R LEY  ISGYQ             LY LG RN++V  LPP+GC+PI  T  F++  
Sbjct: 184 IPSRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-I 243

Query: 243 DRKCLQNQNSDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAF 302
            R CL++ N DS  YN  L  LL  ++  LPGSK  Y D+YN +++MI +P KY      
Sbjct: 244 FRFCLEHHNKDSVLYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKY------ 303

Query: 303 HRTSFIQTTKGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICN 320
               F +T +GCC TG  ET  + N  +P C+   ++LF DS+HP+E+ YN I N
Sbjct: 304 ---GFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFLFFDSIHPSEATYNVIGN 348

BLAST of PI0021513 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 157/349 (44.99%), Postives = 210/349 (60.17%), Query Frame = 0

Query: 15  ACN-AVEPKTSRFFPAILVFGDSTIDPGNNNY-ILTLFRANFRPYGRDFPGQIPTGRFSN 74
           +CN A    T   FPAIL+FGDST+D GNNNY   T+F+A   PYG D PG    GR+SN
Sbjct: 19  SCNVAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSN 78

Query: 75  GKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTISER------ 134
           GK++ D++AS+L IKE VP FL P +S  D+ TGVSF S G+GYDD ++  S+       
Sbjct: 79  GKVISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQ 138

Query: 135 -------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY-NISGYQ- 194
                        IVG+++A +I+++ L V+SAG NDF+ NFYDIP R LEY  I GYQ 
Sbjct: 139 PSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQE 198

Query: 195 -----------GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYN 254
                       LY LGCRNI+V  LPP+GC+PIQ T   ++   R C++ +N DS  YN
Sbjct: 199 FILKRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYN 258

Query: 255 HNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETG 314
             L K L ++Q  LPGS   Y ++Y+ ++DMI +P KY          F +T KGCC TG
Sbjct: 259 QKLVKKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKY---------GFKETKKGCCGTG 318

Query: 315 LGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYL 330
             ET  + NP T TC     +LF DS+HP+E+AYN+I N +  Q+  ++
Sbjct: 319 YLETTFMCNPLTKTCPNHSDHLFWDSIHPSEAAYNYIGNFVDAQIRGWI 357

BLAST of PI0021513 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-76
Identity = 158/348 (45.40%), Postives = 206/348 (59.20%), Query Frame = 0

Query: 15  ACNAVEPKTSRFFPAILVFGDSTIDPGNNNYI-LTLFRANFRPYGRDFPGQIPTGRFSNG 74
           +CNA    T   FPAIL+FGDST D GNNNY    +F+AN  PYG D PG    GRFSNG
Sbjct: 19  SCNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNG 78

Query: 75  KLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTISER------- 134
           KL+ D+++++L IKEFVP FL P +SD D+ TGV F S G+GYDD T+  S+        
Sbjct: 79  KLISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQP 138

Query: 135 ------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY-NISGYQG- 194
                       IVG+++A +I+++ L V+SAG NDF+ NFYDIP R LEY  I GYQ  
Sbjct: 139 SMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDF 198

Query: 195 -----------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNH 254
                      LY LGCRNI+V  LPP+GC+PIQ T   +      C++ +N DS  YN 
Sbjct: 199 VLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQ 258

Query: 255 NLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGL 314
            L K L ++Q  LPGSK  Y ++Y+ V+DMI +P KY          F +T KGCC TG 
Sbjct: 259 KLVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKY---------GFKETKKGCCGTGY 318

Query: 315 GETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYL 330
            ET  L    + TC     +LF DS+HP+E+AY ++ N I  Q+  +L
Sbjct: 319 LETSFLCTSLSKTCPNHSDHLFWDSIHPSEAAYKYLGNFIDAQIQEWL 356

BLAST of PI0021513 vs. ExPASy TrEMBL
Match: A0A5D3C8B7 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00300 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.4e-163
Identity = 294/363 (80.99%), Postives = 305/363 (84.02%), Query Frame = 0

Query: 5   VVTIWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQ 64
           V   WLLLSKACNAVEPK SR FPAILVFGDSTIDPGNNNYILTLFRANF PYGRDFPGQ
Sbjct: 11  VYMTWLLLSKACNAVEPKNSRSFPAILVFGDSTIDPGNNNYILTLFRANFHPYGRDFPGQ 70

Query: 65  IPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS 124
           IPTGRFSNGKLVPDLLASRLGIKE VPAFLDP LSDDDLRTGVSFGS GSGYDDLTTTIS
Sbjct: 71  IPTGRFSNGKLVPDLLASRLGIKELVPAFLDPNLSDDDLRTGVSFGSAGSGYDDLTTTIS 130

Query: 125 -------------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY 184
                              +RIVGEEEAKKIL DGLTVVSAGTNDF+FNFYDIPRRSLEY
Sbjct: 131 GVIPVLKQIDLFENYIKKLKRIVGEEEAKKILGDGLTVVSAGTNDFIFNFYDIPRRSLEY 190

Query: 185 NISGY------------QGLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQN 244
           NI+GY            QGLYQLGCRN+IVA LPPIGCIP+Q+TLAFQDPKDRKC+QNQN
Sbjct: 191 NINGYQAFVQNRLQSFIQGLYQLGCRNMIVAGLPPIGCIPVQETLAFQDPKDRKCIQNQN 250

Query: 245 SDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTT 304
           SDSQNYN+NLTKLLHDLQELLPGSKIHYIDIYN +IDMIN P+KY          FIQTT
Sbjct: 251 SDSQNYNNNLTKLLHDLQELLPGSKIHYIDIYNPMIDMINTPRKY---------GFIQTT 310

Query: 305 KGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQNP 337
           KGCC TGLGETG L NPKTPTCEESKKYLF DSVHPTESAYNFIC+NIVKQLGSYLIQNP
Sbjct: 311 KGCCGTGLGETGALCNPKTPTCEESKKYLFWDSVHPTESAYNFICDNIVKQLGSYLIQNP 364

BLAST of PI0021513 vs. ExPASy TrEMBL
Match: A0A1S3CKF6 (GDSL esterase/lipase At1g06990 OS=Cucumis melo OX=3656 GN=LOC103501965 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.4e-163
Identity = 294/363 (80.99%), Postives = 305/363 (84.02%), Query Frame = 0

Query: 5   VVTIWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQ 64
           V   WLLLSKACNAVEPK SR FPAILVFGDSTIDPGNNNYILTLFRANF PYGRDFPGQ
Sbjct: 11  VYMTWLLLSKACNAVEPKNSRSFPAILVFGDSTIDPGNNNYILTLFRANFHPYGRDFPGQ 70

Query: 65  IPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS 124
           IPTGRFSNGKLVPDLLASRLGIKE VPAFLDP LSDDDLRTGVSFGS GSGYDDLTTTIS
Sbjct: 71  IPTGRFSNGKLVPDLLASRLGIKELVPAFLDPNLSDDDLRTGVSFGSAGSGYDDLTTTIS 130

Query: 125 -------------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY 184
                              +RIVGEEEAKKIL DGLTVVSAGTNDF+FNFYDIPRRSLEY
Sbjct: 131 GVIPVLKQIDLFENYIKKLKRIVGEEEAKKILGDGLTVVSAGTNDFIFNFYDIPRRSLEY 190

Query: 185 NISGY------------QGLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQN 244
           NI+GY            QGLYQLGCRN+IVA LPPIGCIP+Q+TLAFQDPKDRKC+QNQN
Sbjct: 191 NINGYQAFVQNRLQSFIQGLYQLGCRNMIVAGLPPIGCIPVQETLAFQDPKDRKCIQNQN 250

Query: 245 SDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTT 304
           SDSQNYN+NLTKLLHDLQELLPGSKIHYIDIYN +IDMIN P+KY          FIQTT
Sbjct: 251 SDSQNYNNNLTKLLHDLQELLPGSKIHYIDIYNPMIDMINTPRKY---------GFIQTT 310

Query: 305 KGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQNP 337
           KGCC TGLGETG L NPKTPTCEESKKYLF DSVHPTESAYNFIC+NIVKQLGSYLIQNP
Sbjct: 311 KGCCGTGLGETGALCNPKTPTCEESKKYLFWDSVHPTESAYNFICDNIVKQLGSYLIQNP 364

BLAST of PI0021513 vs. ExPASy TrEMBL
Match: A0A6J1J8U7 (GDSL esterase/lipase At1g06990-like OS=Cucurbita maxima OX=3661 GN=LOC111484498 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 6.0e-138
Identity = 248/356 (69.66%), Postives = 278/356 (78.09%), Query Frame = 0

Query: 8   IWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPT 67
           IWLLLS+ACNAVEPKTSR FPAIL+FGDSTIDPGNNNYI TLF+AN+ PYGRDFPG I T
Sbjct: 2   IWLLLSRACNAVEPKTSRLFPAILIFGDSTIDPGNNNYIPTLFKANYNPYGRDFPGHIAT 61

Query: 68  GRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS--- 127
           GRFSNGKL+PD+LAS LGIKE VPAFLDP LSD DLRTGVSFGS GSGYDDLTT+ S   
Sbjct: 62  GRFSNGKLIPDILASTLGIKELVPAFLDPMLSDGDLRTGVSFGSAGSGYDDLTTSRSRVI 121

Query: 128 ----------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNIS 187
                           ERIVGEE+AK+IL   L +VSAG+NDF+ NFYDIP RSLE+NIS
Sbjct: 122 PMLKQIDLFKNYIQRLERIVGEEKAKRILKGALVIVSAGSNDFILNFYDIPSRSLEFNIS 181

Query: 188 GYQ------------GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDS 247
           GYQ            GLYQLGCR I V  LPPIGC+PIQ+T+AFQDPK+RKCL+NQNSDS
Sbjct: 182 GYQAFVQNRLQSLIKGLYQLGCRTIFVVGLPPIGCLPIQETVAFQDPKNRKCLENQNSDS 241

Query: 248 QNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGC 307
           Q YNH LT LLH LQ LLPGSKIH++DIYN +IDMINHP++Y          F+QTTKGC
Sbjct: 242 QIYNHKLTTLLHGLQSLLPGSKIHHVDIYNPLIDMINHPKRY---------GFVQTTKGC 301

Query: 308 CETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQN 333
           C TGL ETGPL NP+TPTCE+ KKYLF DSVHPTESAY FI ++I+KQLG   I+N
Sbjct: 302 CGTGLAETGPLCNPETPTCEDPKKYLFWDSVHPTESAYYFIADSILKQLGFKQIRN 348

BLAST of PI0021513 vs. ExPASy TrEMBL
Match: A0A6J1FUU4 (GDSL esterase/lipase At1g06990-like OS=Cucurbita moschata OX=3662 GN=LOC111448638 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.3e-118
Identity = 221/342 (64.62%), Postives = 251/342 (73.39%), Query Frame = 0

Query: 22  KTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPTGRFSNGKLVPDLLA 81
           K   FF   L FGDST+DPGNNNYI TLF+AN+ PYGRDFPG I TGRFSNGKL+PD+LA
Sbjct: 13  KLLEFFLPFL-FGDSTVDPGNNNYIPTLFKANYNPYGRDFPGHIATGRFSNGKLIPDILA 72

Query: 82  SRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS----------------- 141
           S  GIKE VP FLDP LSD DLRTGVSFGS GSGYDDLT + S                 
Sbjct: 73  SIFGIKELVPGFLDPMLSDGDLRTGVSFGSAGSGYDDLTASRSRVIPMLKQIDLFKNYIQ 132

Query: 142 --ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNISGYQ----------- 201
             ERIVGEE+AK+IL+  L +V AG+NDF+ NFYDIP RSLE+NISGYQ           
Sbjct: 133 RLERIVGEEKAKRILNGALVIVRAGSNDFILNFYDIPSRSLEFNISGYQAFVQNRLQSLI 192

Query: 202 -GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNHNLTKLLHDL 261
            GLYQLGCR I V  LPPIGC+PIQ+T+AFQDPK+RKCL+NQNSDSQ YNH LT LLH L
Sbjct: 193 KGLYQLGCRTIFVVGLPPIGCLPIQETVAFQDPKNRKCLENQNSDSQIYNHKLTTLLHGL 252

Query: 262 QELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGLGETGPLGNP 321
           Q LLPGSKIH++DIYN +IDMINHP++Y          F+QTTKGC  TGL ETG L NP
Sbjct: 253 QSLLPGSKIHHVDIYNPLIDMINHPKRY---------GFVQTTKGCWGTGLAETGLLCNP 312

Query: 322 KTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQN 333
           +T TCE+ KKYLF DSVHPTESAY  I ++I+KQLG   I+N
Sbjct: 313 ETTTCEDPKKYLFWDSVHPTESAYYLIADSILKQLGLNRIRN 344

BLAST of PI0021513 vs. ExPASy TrEMBL
Match: A0A0A0LNE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431080 PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.4e-110
Identity = 198/353 (56.09%), Postives = 255/353 (72.24%), Query Frame = 0

Query: 7   TIWLL--LSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQ 66
           TIWLL  L+K C+++EPKT+  FPAIL+FGDST+D GNNN+I T+F+ N+ PYG++FPG 
Sbjct: 15  TIWLLVVLTKPCSSLEPKTTPSFPAILIFGDSTVDTGNNNFIPTIFKGNYSPYGKNFPGH 74

Query: 67  IPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS 126
           + TGRFS+GKL+PD++ASRLGIKE VP FLDPKLS+DD++TGVSF S G+G+DDLT  IS
Sbjct: 75  LATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTGFDDLTAAIS 134

Query: 127 -------------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY 186
                              + +VG +E+K+I+++ L V+SAGTND   NFYD+P R L+Y
Sbjct: 135 KVIPVMKQIDHFKNYIQRLQGVVGVDESKRIINNALVVISAGTNDLNINFYDLPTRQLQY 194

Query: 187 NISGYQG------------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQN 246
           NISGYQ             +YQLGCRNI+VA LPP+GC+PIQ+T+AF++P  R CL++QN
Sbjct: 195 NISGYQDFLQNRLQSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPLKRNCLKDQN 254

Query: 247 SDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTT 306
           SDS  YN  L+KLL +LQ  L GSKI Y DIY  +IDM+N+PQKY          F  T 
Sbjct: 255 SDSVAYNQKLSKLLTNLQPQLAGSKILYADIYTPLIDMLNNPQKY---------GFDHTN 314

Query: 307 KGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLG 327
           +GCC TGL E GPL NPKTPTCE S K++F DS+HPTE+AY FI   ++K+LG
Sbjct: 315 RGCCGTGLVEAGPLCNPKTPTCENSSKFMFWDSIHPTEAAYKFIAEALLKKLG 358

BLAST of PI0021513 vs. NCBI nr
Match: XP_008463972.1 (PREDICTED: GDSL esterase/lipase At1g06990 [Cucumis melo] >KAA0059945.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07590.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 294/363 (80.99%), Postives = 305/363 (84.02%), Query Frame = 0

Query: 5   VVTIWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQ 64
           V   WLLLSKACNAVEPK SR FPAILVFGDSTIDPGNNNYILTLFRANF PYGRDFPGQ
Sbjct: 11  VYMTWLLLSKACNAVEPKNSRSFPAILVFGDSTIDPGNNNYILTLFRANFHPYGRDFPGQ 70

Query: 65  IPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS 124
           IPTGRFSNGKLVPDLLASRLGIKE VPAFLDP LSDDDLRTGVSFGS GSGYDDLTTTIS
Sbjct: 71  IPTGRFSNGKLVPDLLASRLGIKELVPAFLDPNLSDDDLRTGVSFGSAGSGYDDLTTTIS 130

Query: 125 -------------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY 184
                              +RIVGEEEAKKIL DGLTVVSAGTNDF+FNFYDIPRRSLEY
Sbjct: 131 GVIPVLKQIDLFENYIKKLKRIVGEEEAKKILGDGLTVVSAGTNDFIFNFYDIPRRSLEY 190

Query: 185 NISGY------------QGLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQN 244
           NI+GY            QGLYQLGCRN+IVA LPPIGCIP+Q+TLAFQDPKDRKC+QNQN
Sbjct: 191 NINGYQAFVQNRLQSFIQGLYQLGCRNMIVAGLPPIGCIPVQETLAFQDPKDRKCIQNQN 250

Query: 245 SDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTT 304
           SDSQNYN+NLTKLLHDLQELLPGSKIHYIDIYN +IDMIN P+KY          FIQTT
Sbjct: 251 SDSQNYNNNLTKLLHDLQELLPGSKIHYIDIYNPMIDMINTPRKY---------GFIQTT 310

Query: 305 KGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQNP 337
           KGCC TGLGETG L NPKTPTCEESKKYLF DSVHPTESAYNFIC+NIVKQLGSYLIQNP
Sbjct: 311 KGCCGTGLGETGALCNPKTPTCEESKKYLFWDSVHPTESAYNFICDNIVKQLGSYLIQNP 364

BLAST of PI0021513 vs. NCBI nr
Match: XP_038900806.1 (GDSL esterase/lipase At1g06990-like [Benincasa hispida])

HSP 1 Score: 552.0 bits (1421), Expect = 3.6e-153
Identity = 274/360 (76.11%), Postives = 296/360 (82.22%), Query Frame = 0

Query: 8   IWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPT 67
           IWLLLSK CNAVEPKTSR FPAI +FGDST+D GNNNYILTLF+AN++PYGRDFPGQIPT
Sbjct: 14  IWLLLSKPCNAVEPKTSRAFPAIFIFGDSTVDTGNNNYILTLFKANYKPYGRDFPGQIPT 73

Query: 68  GRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS--- 127
           GRFSNGKLVPD+LAS LGIKE VPAFLDPKLSDDDLRTGVSFGS GSGYDDLTTTIS   
Sbjct: 74  GRFSNGKLVPDILASLLGIKELVPAFLDPKLSDDDLRTGVSFGSAGSGYDDLTTTISGVI 133

Query: 128 ----------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNIS 187
                           ERIVGEEEAK ILS GLTVVSAGTNDFLFNFYDIPRRSLEY+IS
Sbjct: 134 PVLKQIDLFENYIQRLERIVGEEEAKTILSGGLTVVSAGTNDFLFNFYDIPRRSLEYDIS 193

Query: 188 GYQ------------GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDS 247
           GYQ            GLYQLGCRN++VA LPPIGC+PIQ+TLAFQDPK+RKC+++QNSDS
Sbjct: 194 GYQAFVHGRLQSFIKGLYQLGCRNMLVAGLPPIGCLPIQETLAFQDPKNRKCVEHQNSDS 253

Query: 248 QNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGC 307
           QNYNH LTKLLHDLQELLPGS IHY+DIYN VID+I HPQKY          F+QTTKGC
Sbjct: 254 QNYNHKLTKLLHDLQELLPGSTIHYVDIYNPVIDIIKHPQKY---------GFVQTTKGC 313

Query: 308 CETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQNPSSP 337
           C TGL E+GPL NPKTPTCEE KKYLF DSVHPTES Y F+ N+I+KQLGSYLIQN SSP
Sbjct: 314 CGTGLAESGPLCNPKTPTCEEPKKYLFWDSVHPTESTYYFVSNSILKQLGSYLIQNRSSP 364

BLAST of PI0021513 vs. NCBI nr
Match: XP_022986897.1 (GDSL esterase/lipase At1g06990-like [Cucurbita maxima])

HSP 1 Score: 500.4 bits (1287), Expect = 1.2e-137
Identity = 248/356 (69.66%), Postives = 278/356 (78.09%), Query Frame = 0

Query: 8   IWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPT 67
           IWLLLS+ACNAVEPKTSR FPAIL+FGDSTIDPGNNNYI TLF+AN+ PYGRDFPG I T
Sbjct: 2   IWLLLSRACNAVEPKTSRLFPAILIFGDSTIDPGNNNYIPTLFKANYNPYGRDFPGHIAT 61

Query: 68  GRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS--- 127
           GRFSNGKL+PD+LAS LGIKE VPAFLDP LSD DLRTGVSFGS GSGYDDLTT+ S   
Sbjct: 62  GRFSNGKLIPDILASTLGIKELVPAFLDPMLSDGDLRTGVSFGSAGSGYDDLTTSRSRVI 121

Query: 128 ----------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNIS 187
                           ERIVGEE+AK+IL   L +VSAG+NDF+ NFYDIP RSLE+NIS
Sbjct: 122 PMLKQIDLFKNYIQRLERIVGEEKAKRILKGALVIVSAGSNDFILNFYDIPSRSLEFNIS 181

Query: 188 GYQ------------GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDS 247
           GYQ            GLYQLGCR I V  LPPIGC+PIQ+T+AFQDPK+RKCL+NQNSDS
Sbjct: 182 GYQAFVQNRLQSLIKGLYQLGCRTIFVVGLPPIGCLPIQETVAFQDPKNRKCLENQNSDS 241

Query: 248 QNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGC 307
           Q YNH LT LLH LQ LLPGSKIH++DIYN +IDMINHP++Y          F+QTTKGC
Sbjct: 242 QIYNHKLTTLLHGLQSLLPGSKIHHVDIYNPLIDMINHPKRY---------GFVQTTKGC 301

Query: 308 CETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQN 333
           C TGL ETGPL NP+TPTCE+ KKYLF DSVHPTESAY FI ++I+KQLG   I+N
Sbjct: 302 CGTGLAETGPLCNPETPTCEDPKKYLFWDSVHPTESAYYFIADSILKQLGFKQIRN 348

BLAST of PI0021513 vs. NCBI nr
Match: XP_023512080.1 (GDSL esterase/lipase At1g06990-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 493.4 bits (1269), Expect = 1.5e-135
Identity = 244/356 (68.54%), Postives = 276/356 (77.53%), Query Frame = 0

Query: 8   IWLLLSKACNAVEPKTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPT 67
           IWL LS+ACNAVEPKTSR FPAIL+FGDST+DPGNNNYI TLF+AN+ PYGRDFPG I T
Sbjct: 14  IWLPLSRACNAVEPKTSRVFPAILIFGDSTVDPGNNNYIPTLFKANYNPYGRDFPGHIAT 73

Query: 68  GRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS--- 127
           GRFSNGKL+PD+LAS LGIKE VPAFLDP LSD DLRTGVSFGS GSGYDDLT + S   
Sbjct: 74  GRFSNGKLIPDILASTLGIKELVPAFLDPMLSDGDLRTGVSFGSAGSGYDDLTASRSRVI 133

Query: 128 ----------------ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNIS 187
                           ERIVGEE+AK+IL+  L +VSAG+NDF+ NFYDIP RSLE+NIS
Sbjct: 134 PMLKQIDLFKNYIQRLERIVGEEKAKRILNGALVIVSAGSNDFILNFYDIPSRSLEFNIS 193

Query: 188 GY------------QGLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDS 247
           GY            QGLYQLGCR I +  LPPIGC+PIQ+T+AFQDPK+RKCL+NQNSDS
Sbjct: 194 GYQAFVQNRLQSLIQGLYQLGCRTIFIVGLPPIGCLPIQETVAFQDPKNRKCLENQNSDS 253

Query: 248 QNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGC 307
           Q YNH LT LLH LQ LLPGSKIH++DIYN +IDMINHP+         R  F+QTTKGC
Sbjct: 254 QIYNHKLTTLLHGLQSLLPGSKIHHVDIYNPLIDMINHPK---------RCGFVQTTKGC 313

Query: 308 CETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQN 333
           C TGL ETGPL NP+TPTCE+ KKYLF DSVHPTESAY FI ++I+KQLG   I+N
Sbjct: 314 CGTGLAETGPLCNPETPTCEDPKKYLFWDSVHPTESAYYFIADSILKQLGFNQIRN 360

BLAST of PI0021513 vs. NCBI nr
Match: XP_022944092.1 (GDSL esterase/lipase At1g06990-like [Cucurbita moschata])

HSP 1 Score: 434.1 bits (1115), Expect = 1.1e-117
Identity = 221/342 (64.62%), Postives = 251/342 (73.39%), Query Frame = 0

Query: 22  KTSRFFPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPTGRFSNGKLVPDLLA 81
           K   FF   L FGDST+DPGNNNYI TLF+AN+ PYGRDFPG I TGRFSNGKL+PD+LA
Sbjct: 13  KLLEFFLPFL-FGDSTVDPGNNNYIPTLFKANYNPYGRDFPGHIATGRFSNGKLIPDILA 72

Query: 82  SRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTIS----------------- 141
           S  GIKE VP FLDP LSD DLRTGVSFGS GSGYDDLT + S                 
Sbjct: 73  SIFGIKELVPGFLDPMLSDGDLRTGVSFGSAGSGYDDLTASRSRVIPMLKQIDLFKNYIQ 132

Query: 142 --ERIVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNISGYQ----------- 201
             ERIVGEE+AK+IL+  L +V AG+NDF+ NFYDIP RSLE+NISGYQ           
Sbjct: 133 RLERIVGEEKAKRILNGALVIVRAGSNDFILNFYDIPSRSLEFNISGYQAFVQNRLQSLI 192

Query: 202 -GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNHNLTKLLHDL 261
            GLYQLGCR I V  LPPIGC+PIQ+T+AFQDPK+RKCL+NQNSDSQ YNH LT LLH L
Sbjct: 193 KGLYQLGCRTIFVVGLPPIGCLPIQETVAFQDPKNRKCLENQNSDSQIYNHKLTTLLHGL 252

Query: 262 QELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGLGETGPLGNP 321
           Q LLPGSKIH++DIYN +IDMINHP++Y          F+QTTKGC  TGL ETG L NP
Sbjct: 253 QSLLPGSKIHHVDIYNPLIDMINHPKRY---------GFVQTTKGCWGTGLAETGLLCNP 312

Query: 322 KTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYLIQN 333
           +T TCE+ KKYLF DSVHPTESAY  I ++I+KQLG   I+N
Sbjct: 313 ETTTCEDPKKYLFWDSVHPTESAYYLIADSILKQLGLNRIRN 344

BLAST of PI0021513 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 6.0e-82
Identity = 160/330 (48.48%), Postives = 202/330 (61.21%), Query Frame = 0

Query: 27  FPAILVFGDSTIDPGNNNYILTLFRANFRPYGRDFPGQIPTGRFSNGKLVPDLLASRLGI 86
           FPAILVFGDSTID GNNNYI T  RANF PYG +FPG   TGRFSNGKL+PD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 87  KEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDL----TTTIS---------------ERI 146
           K+ VP FLDP LSD D+ TGV F S GSGYD+L    T+T+S                +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 147 VGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEYNISGYQG------------LYQ 206
           VG+E+A  I+S+ L +VS+GTNDF  N YD P R  +  + GYQ             LY 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 207 LGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNHNLTKLLHDLQELLP 266
           +GCR I+V  LPP+GC+PIQ T+A Q   +R+C+  QNSDSQ +N  L   L ++Q  L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 267 GSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGLGETGPLGNPKTPTC 326
           GS I Y DIY  + DM  +PQ+Y L          +TT+GCC TG  E   L N  T  C
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGL---------KETTRGCCGTGEIELAYLCNALTRIC 334

BLAST of PI0021513 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 4.8e-79
Identity = 160/355 (45.07%), Postives = 216/355 (60.85%), Query Frame = 0

Query: 3   SRVVTIWLLLSKA----CNAVEPKTSR-FFPAILVFGDSTIDPGNNNYIL-TLFRANFRP 62
           S+ +T+ L ++      CNA    T++  FPAIL+FGDST+D GNNNY L T+FRA   P
Sbjct: 4   SKAITLTLFIATTLLAPCNAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFP 63

Query: 63  YGRDFPGQIPTGRFSNGKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGY 122
           YG D P     GRFSNGKL+ D++A++L IKEF+P FL P LSD D+ TGV F S G+GY
Sbjct: 64  YGMDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGY 123

Query: 123 DDLTT------TISER-------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYD 182
           DDLT+       +SE+             IVG+++A +I+++   VVSAG NDF+ N+Y+
Sbjct: 124 DDLTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYE 183

Query: 183 IPRRSLEYN-ISGYQG------------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPK 242
           IP R LEY  ISGYQ             LY LG RN++V  LPP+GC+PI  T  F++  
Sbjct: 184 IPSRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-I 243

Query: 243 DRKCLQNQNSDSQNYNHNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAF 302
            R CL++ N DS  YN  L  LL  ++  LPGSK  Y D+YN +++MI +P KY      
Sbjct: 244 FRFCLEHHNKDSVLYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKY------ 303

Query: 303 HRTSFIQTTKGCCETGLGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICN 320
               F +T +GCC TG  ET  + N  +P C+   ++LF DS+HP+E+ YN I N
Sbjct: 304 ---GFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFLFFDSIHPSEATYNVIGN 348

BLAST of PI0021513 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 291.6 bits (745), Expect = 8.2e-79
Identity = 157/349 (44.99%), Postives = 210/349 (60.17%), Query Frame = 0

Query: 15  ACN-AVEPKTSRFFPAILVFGDSTIDPGNNNY-ILTLFRANFRPYGRDFPGQIPTGRFSN 74
           +CN A    T   FPAIL+FGDST+D GNNNY   T+F+A   PYG D PG    GR+SN
Sbjct: 19  SCNVAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSN 78

Query: 75  GKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTISER------ 134
           GK++ D++AS+L IKE VP FL P +S  D+ TGVSF S G+GYDD ++  S+       
Sbjct: 79  GKVISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQ 138

Query: 135 -------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY-NISGYQ- 194
                        IVG+++A +I+++ L V+SAG NDF+ NFYDIP R LEY  I GYQ 
Sbjct: 139 PSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQE 198

Query: 195 -----------GLYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYN 254
                       LY LGCRNI+V  LPP+GC+PIQ T   ++   R C++ +N DS  YN
Sbjct: 199 FILKRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYN 258

Query: 255 HNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETG 314
             L K L ++Q  LPGS   Y ++Y+ ++DMI +P KY          F +T KGCC TG
Sbjct: 259 QKLVKKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKY---------GFKETKKGCCGTG 318

Query: 315 LGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYL 330
             ET  + NP T TC     +LF DS+HP+E+AYN+I N +  Q+  ++
Sbjct: 319 YLETTFMCNPLTKTCPNHSDHLFWDSIHPSEAAYNYIGNFVDAQIRGWI 357

BLAST of PI0021513 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.2e-77
Identity = 158/348 (45.40%), Postives = 206/348 (59.20%), Query Frame = 0

Query: 15  ACNAVEPKTSRFFPAILVFGDSTIDPGNNNYI-LTLFRANFRPYGRDFPGQIPTGRFSNG 74
           +CNA    T   FPAIL+FGDST D GNNNY    +F+AN  PYG D PG    GRFSNG
Sbjct: 19  SCNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNG 78

Query: 75  KLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTTTISER------- 134
           KL+ D+++++L IKEFVP FL P +SD D+ TGV F S G+GYDD T+  S+        
Sbjct: 79  KLISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQP 138

Query: 135 ------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPRRSLEY-NISGYQG- 194
                       IVG+++A +I+++ L V+SAG NDF+ NFYDIP R LEY  I GYQ  
Sbjct: 139 SMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDF 198

Query: 195 -----------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYNH 254
                      LY LGCRNI+V  LPP+GC+PIQ T   +      C++ +N DS  YN 
Sbjct: 199 VLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQ 258

Query: 255 NLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETGL 314
            L K L ++Q  LPGSK  Y ++Y+ V+DMI +P KY          F +T KGCC TG 
Sbjct: 259 KLVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKY---------GFKETKKGCCGTGY 318

Query: 315 GETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYL 330
            ET  L    + TC     +LF DS+HP+E+AY ++ N I  Q+  +L
Sbjct: 319 LETSFLCTSLSKTCPNHSDHLFWDSIHPSEAAYKYLGNFIDAQIQEWL 356

BLAST of PI0021513 vs. TAIR 10
Match: AT1G58430.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-76
Identity = 154/349 (44.13%), Postives = 213/349 (61.03%), Query Frame = 0

Query: 15  ACNA-VEPKTSRFFPAILVFGDSTIDPGNNNY-ILTLFRANFRPYGRDFPGQIPTGRFSN 74
           +CNA  + KT   FPAIL+FGDST+D GNNNY   T+FRA   PYG D P   P GRFSN
Sbjct: 20  SCNASAKAKTQPLFPAILIFGDSTVDTGNNNYPSQTIFRAKHVPYGIDLPNHSPNGRFSN 79

Query: 75  GKLVPDLLASRLGIKEFVPAFLDPKLSDDDLRTGVSFGSTGSGYDDLTT------TISER 134
           GK+  D++A++L IK+FVP FL P L+D ++ TGV F S G+GYDD T+       +SE+
Sbjct: 80  GKIFSDIIATKLNIKQFVPPFLQPNLTDQEIVTGVCFASAGAGYDDQTSLTTQAIRVSEQ 139

Query: 135 -------------IVGEEEAKKILSDGLTVVSAGTNDFLFNFYDIPR-RSLEYNISGYQG 194
                        IVG+++A KI+++ L VVSAG NDF+ N+Y++P  R +  +IS YQ 
Sbjct: 140 PNMFKSYIARLKSIVGDKKAMKIINNALVVVSAGPNDFILNYYEVPSWRRMYPSISDYQD 199

Query: 195 ------------LYQLGCRNIIVARLPPIGCIPIQDTLAFQDPKDRKCLQNQNSDSQNYN 254
                       LY LGCR I+V  LPP+GC+PIQ T  F++   R CL+ +N DS  YN
Sbjct: 200 FVLSRLNNFVKELYSLGCRKILVGGLPPMGCLPIQMTAQFRNVL-RFCLEQENRDSVLYN 259

Query: 255 HNLTKLLHDLQELLPGSKIHYIDIYNLVIDMINHPQKYVLFDAFHRTSFIQTTKGCCETG 314
             L KLL   Q  L GSKI Y D+Y+ +++M+ +P KY          F +TT+GCC TG
Sbjct: 260 QKLQKLLPQTQASLTGSKILYSDVYDPMMEMLQNPSKY---------GFKETTRGCCGTG 319

Query: 315 LGETGPLGNPKTPTCEESKKYLFLDSVHPTESAYNFICNNIVKQLGSYL 330
             ET  + N  +  C+   ++LF DS+HP+E+ YN+I N +  ++  +L
Sbjct: 320 FLETSFMCNAYSSMCQNRSEFLFFDSIHPSEATYNYIGNVLDTKIRGWL 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMJ38.5e-8148.48GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2... [more]
Q9SIQ21.8e-7844.82GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3... [more]
Q9SIQ36.7e-7845.07GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2... [more]
O229271.2e-7744.99GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2... [more]
O229181.7e-7645.40GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3... [more]
Match NameE-valueIdentityDescription
A0A5D3C8B75.4e-16380.99GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852... [more]
A0A1S3CKF65.4e-16380.99GDSL esterase/lipase At1g06990 OS=Cucumis melo OX=3656 GN=LOC103501965 PE=3 SV=1[more]
A0A6J1J8U76.0e-13869.66GDSL esterase/lipase At1g06990-like OS=Cucurbita maxima OX=3661 GN=LOC111484498 ... [more]
A0A6J1FUU45.3e-11864.62GDSL esterase/lipase At1g06990-like OS=Cucurbita moschata OX=3662 GN=LOC11144863... [more]
A0A0A0LNE22.4e-11056.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431080 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008463972.11.1e-16280.99PREDICTED: GDSL esterase/lipase At1g06990 [Cucumis melo] >KAA0059945.1 GDSL este... [more]
XP_038900806.13.6e-15376.11GDSL esterase/lipase At1g06990-like [Benincasa hispida][more]
XP_022986897.11.2e-13769.66GDSL esterase/lipase At1g06990-like [Cucurbita maxima][more]
XP_023512080.11.5e-13568.54GDSL esterase/lipase At1g06990-like [Cucurbita pepo subsp. pepo][more]
XP_022944092.11.1e-11764.62GDSL esterase/lipase At1g06990-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G06990.16.0e-8248.48GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G31540.14.8e-7945.07GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G30310.18.2e-7944.99GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G30220.11.2e-7745.40GDSL-like Lipase/Acylhydrolase family protein [more]
AT1G58430.11.0e-7644.13GDSL-like Lipase/Acylhydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 30..317
e-value: 7.1E-30
score: 105.1
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 18..327
e-value: 1.2E-60
score: 207.6
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 26..320
NoneNo IPR availablePANTHERPTHR45642:SF30GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 26..320
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 30..327
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 28..321
e-value: 2.46469E-107
score: 314.168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021513.1PI0021513.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds