PI0021406 (gene) Melon (PI 482460) v1

Overview


Name	PI0021406
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Thiamine thiazole synthase, chloroplastic
Location	chr06: 25846435 .. 25848300 (+)
RNA-Seq Expression	PI0021406
Synteny	PI0021406

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAAATTGTGTGACATTCTTCAATATCCTCTATAAAACCCTTCTTCCTTCCCCTCTTCTTCCCCACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCCAAAATGGCCTCCATTGCCTCTACTCTCACCACCAAGCTCCAAAGACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCTCCTCCTTCTCTCCGCCTCAAATCCACCGCCGCCGCATCTCTCTCCATCTCTGCCTCCGCCTCTCAGCCCCCCTACGATTTAAACCAATTCAAATTCAACCCCATCAAAGAATCCATTGTCTCTCGAGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAACTCAGCAAGAACCCCAATATTCGTATCGCCATCATCGAACAATCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTAAGAAATTCGTTTCCTTTCCAAATTTGAATGGATGCTCTGTTTTTCTTTTCTGTTTTGTTCTTTTCTTGAAAATACCAAAAATTTGAAAATCCCCCAATCAGCTTTCATGCATGCCTTATTTGGACTAAAAAATTTCATTTTTTTTATAACAGAGATCTGAAATAAAGAAACCCAGAAAGTAAAATCATTAATTTGAGAGGAAATAGTATAGAGATCTTGTGATCCTCTAAAAAATTAGATTAATTTTCATCGGAGACAAATATTCTGATCTTCTTAATCTGATTGATTTTGGTTAAATTTAATGTTTTATAGGTGGTTCGTAAGCCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGAGGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTGACCAACTGGGCGCTGGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTCGGCGCTACGGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCAGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGTAAACGAACTAGAAAGAGATTACTGCTTTAGCCCATTTGATAATCATGATACCGTAAAATATAGCAAAATTTAGATGAGTTTATAAAAAGATTTTGTTATACCCATATTTATTAGTCTTAAACTGTGGCATTCATAGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCGCACTTAGCGTTGAAGTCATTGGGAGAGACGAACAGCATTGGGGATGAAGAAGGAGAGATGGGAGAAAGAAAAGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCGGCCGGAGAGTCACCGGAGGTTGCAGATGCTTAAAGTTTTTAGTGGCGGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTCTGTTTGTAGTGAGTTTTCAGTGATGAAAAGGGGGGTTGGTTGTGTTTGGCTTTTTTGTGTTTTCATAACACAATGGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCTTTCTCTCTCTTTTTTACTTTTTTTTTTTTTTTTTTTT

mRNA sequence

GTAAAATTGTGTGACATTCTTCAATATCCTCTATAAAACCCTTCTTCCTTCCCCTCTTCTTCCCCACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCCAAAATGGCCTCCATTGCCTCTACTCTCACCACCAAGCTCCAAAGACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCTCCTCCTTCTCTCCGCCTCAAATCCACCGCCGCCGCATCTCTCTCCATCTCTGCCTCCGCCTCTCAGCCCCCCTACGATTTAAACCAATTCAAATTCAACCCCATCAAAGAATCCATTGTCTCTCGAGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAACTCAGCAAGAACCCCAATATTCGTATCGCCATCATCGAACAATCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTTCGTAAGCCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGAGGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTGACCAACTGGGCGCTGGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTCGGCGCTACGGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCAGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCGCACTTAGCGTTGAAGTCATTGGGAGAGACGAACAGCATTGGGGATGAAGAAGGAGAGATGGGAGAAAGAAAAGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCGGCCGGAGAGTCACCGGAGGTTGCAGATGCTTAAAGTTTTTAGTGGCGGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTCTGTTTGTAGTGAGTTTTCAGTGATGAAAAGGGGGGTTGGTTGTGTTTGGCTTTTTTGTGTTTTCATAACACAATGGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCTTTCTCTCTCTTTTTTACTTTTTTTTTTTTTTTTTTTT

Coding sequence (CDS)

ATGGCCTCCATTGCCTCTACTCTCACCACCAAGCTCCAAAGACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCTCCTCCTTCTCTCCGCCTCAAATCCACCGCCGCCGCATCTCTCTCCATCTCTGCCTCCGCCTCTCAGCCCCCCTACGATTTAAACCAATTCAAATTCAACCCCATCAAAGAATCCATTGTCTCTCGAGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAACTCAGCAAGAACCCCAATATTCGTATCGCCATCATCGAACAATCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTTCGTAAGCCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGAGGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTGACCAACTGGGCGCTGGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTCGGCGCTACGGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCAGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCGCACTTAGCGTTGAAGTCATTGGGAGAGACGAACAGCATTGGGGATGAAGAAGGAGAGATGGGAGAAAGAAAAGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCGGCCGGAGAGTCACCGGAGGTTGCAGATGCTTAA

Protein sequence

MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVADA

Homology

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BLAST of PI0021406 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 6.0e-157
Identity = 296/365 (81.10%), Postives = 321/365 (87.95%), Query Frame = 0


Query: 1   MASIAST--LTTKLQRPSLLHDSSFHGTPLASPPSLRL---KSTAAASLSISASASQPPY 60
           MA++AST    +     + L DSSFHG P+ SP  LRL   KS+   +LSISASAS PPY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSISASAS-PPY 60

Query: 61  DLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN FKF+PIKESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPNI+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQ++YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESP 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N++           VG    EL+LAA +S 
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG-------TYVGGVHPELILAAADSA 356

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BLAST of PI0021406 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 6.4e-151
Identity = 285/361 (78.95%), Postives = 315/361 (87.26%), Query Frame = 0


Query: 4   IASTLTTKLQRPSLLHD--SSFHGTPLASPPSLRLKST-AAASLSISAS-ASQPPYDLNQ 63
           +ASTLT+K QR +L  +  SSF+GTPLA P S+R++ T A A  SIS S A  PPYDL  
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSPPYDLKA 60

Query: 64  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 123
           F F+PIKESIVSREMTRRYM DMITYADTDV++VGAGS+GL C YELSKNP++++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIEQ 120

Query: 124 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 183
           SVSPGGGAWLGGQLFS MVVRKPAHLFLDE+G+EYDEQ++YVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLA 180

Query: 184 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 243
           RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 244 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 303
           PFGATGVK L+SIG+ID+VPGMKALDMN AEDAIVR TRE+VPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAPR 300

Query: 304 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 361
           MGPTFGAMMISGQKAAHLALK+LG  N++           VG    EL+LAA +S E+AD
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGLPNALDGS-------YVGGIHPELILAAADSAEIAD 352

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BLAST of PI0021406 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.0e-148
Identity = 278/361 (77.01%), Postives = 307/361 (85.04%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPL-ASPPSLRLKSTAAASLSISASASQPPYDLNQ 60
           MA+IASTL+    +P  L DSSFHG+ + A+P S+ LK       S S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR-----SFSVRATTAGYDLNA 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQ+ YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 360
           MGPTFGAMMISGQKA  LALK+LG  N+I   +G +    VG    EL+LAA +S E  D
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAI---DGTL----VGNLSPELVLAAADSAETVD 349

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BLAST of PI0021406 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.3e-148
Identity = 280/363 (77.13%), Postives = 311/363 (85.67%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPS---LLHDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDL 60
           MAS+A+TLT+    P      + SSFHG+P++        S  + ++S+S   S  PYDL
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPISYRVLPIKVSHQSPTISMS---SVSPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAII 120
             FKF PIKESIV+REMTRRYM DMITYADTDV+IVGAGSAGLSCAYELSKNP+IR+AII
Sbjct: 61  QSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEV 360
           PRMGPTFGAMMISGQKAAHLAL++LG+ N+I   +G   E +    + EL+LAA E+ E+
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALRALGQPNAI---DGNYTEAET--MQPELILAAAETGEI 355

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BLAST of PI0021406 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 6.6e-148
Identity = 271/362 (74.86%), Postives = 311/362 (85.91%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLL--HDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDLN 60
           MA++ S++ +K  + S+   H SSFHG P+A+    RL    +  ++++ +A+  PYDL 
Sbjct: 1   MATLTSSICSK-PKASVFDPHKSSFHGVPIAT--QARLSPVKSTPVNLAVTAAAMPYDLR 60

Query: 61  QFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIE 120
            FKF PIKESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNP++++AIIE
Sbjct: 61  SFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           ARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRL+S+G+IDSVPGMKALDMNTAED IVR TREVVPGMIVTGMEVAEIDG+P
Sbjct: 241 GPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGSP 300

Query: 301 RMGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVA 360
           RMGPTFGAMMISGQKAAHLALKSLG  N++       G     L  E +L AA ++ E+A
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGLPNALD------GTYIGNLHPELVLAAAADAAEIA 353

[Close]

BLAST of PI0021406 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.0e-191
Identity = 350/360 (97.22%), Postives = 356/360 (98.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDLNQF 60
           MASIASTLTTKLQ+PSLLHDSSFHGTPLASP SLRLKSTAA SL+ISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGE NSIGD EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGD-EGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of PI0021406 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.6e-191
Identity = 351/360 (97.50%), Postives = 354/360 (98.33%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASPPSLRLKS AA SL+ISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGE NSIG  EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGG-EGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of PI0021406 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.1e-172
Identity = 324/361 (89.75%), Postives = 342/361 (94.74%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLK-STAAASLSISASASQPPYDLNQ 60
           MASIAS+L TKL RPSLL DSSFHG+PL   PS RLK +TAAASLSISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-DSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 360
           MGPTFGAMMISGQKAAHLALK+LG+ NSI DE+GE G RKV  E  E++LAAGE+PEVAD
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSI-DEKGENGGRKV--EAAEMVLAAGETPEVAD 357

[Close]

BLAST of PI0021406 vs. ExPASy TrEMBL
Match: A0A6J1EVU2 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438464 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 315/361 (87.26%), Postives = 335/361 (92.80%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLK-STAAASLSISASASQPPYDLNQ 60
           MASIAS+L  KL RPSLL +SSFHG+PL   PS RLK +TAAASLSISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPIKLHRPSLL-ESSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 360
           MGPTFGAMMISGQKAAHLALK+LG+TNSI  E          +E  E++LAAGE+PEVAD
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKTNSIDGE----------VEAAEMVLAAGETPEVAD 350

[Close]

BLAST of PI0021406 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.8e-168
Identity = 322/361 (89.20%), Postives = 328/361 (90.86%), Query Frame = 0


Query: 1   MASIASTLTTKLQRP-SLLHDSSFHGTPLA-SPPSLRLKSTAAASLSISASASQPPYDLN 60
           MAS+ASTLTTKLQ P S L DSSFHGTPLA  PPSLRLKST AA  SISAS  Q PYDLN
Sbjct: 1   MASVASTLTTKLQTPSSSLLDSSFHGTPLAPPPPSLRLKSTPAA-FSISAS-QQQPYDLN 60

Query: 61  QFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIE 120
            FKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAIIE
Sbjct: 61  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 180

Query: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 300

Query: 301 RMGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVA 360
           RMGPTFGAMMISGQKAAHLALKSLG  N I DE              +L+LAAGESPEVA
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA------------DLILAAGESPEVA 347

[Close]

BLAST of PI0021406 vs. NCBI nr
Match: XP_004152916.1 (thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_011133 [Cucumis sativus])

HSP 1 Score: 679.1 bits (1751), Expect = 2.1e-191
Identity = 350/360 (97.22%), Postives = 356/360 (98.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDLNQF 60
           MASIASTLTTKLQ+PSLLHDSSFHGTPLASP SLRLKSTAA SL+ISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGE NSIGD EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGD-EGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of PI0021406 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 676.4 bits (1744), Expect = 1.4e-190
Identity = 351/360 (97.50%), Postives = 354/360 (98.33%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLKSTAAASLSISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASPPSLRLKS AA SL+ISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGE NSIG  EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGG-EGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of PI0021406 vs. NCBI nr
Match: XP_038905461.1 (thiamine thiazole synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 653.7 bits (1685), Expect = 9.4e-184
Identity = 346/364 (95.05%), Postives = 351/364 (96.43%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLKS----TAAASLSISASASQPPYD 60
           MASIASTLTTKLQRPSLL DSSF+GTPLA PPSLRLKS    TAA SLSISASASQPPYD
Sbjct: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120
           LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPE 360
           APRMGPTFGAMMISGQKAAHLALKSLGE N I  E+GE+GERKV +EE ELLLAAGESPE
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI--EDGEVGERKV-VEEPELLLAAGESPE 360

[Close]

BLAST of PI0021406 vs. NCBI nr
Match: XP_022985241.1 (thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 615.9 bits (1587), Expect = 2.2e-172
Identity = 324/361 (89.75%), Postives = 342/361 (94.74%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLK-STAAASLSISASASQPPYDLNQ 60
           MASIAS+L TKL RPSLL DSSFHG+PL   PS RLK +TAAASLSISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-DSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 360
           MGPTFGAMMISGQKAAHLALK+LG+ NSI DE+GE G RKV  E  E++LAAGE+PEVAD
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSI-DEKGENGGRKV--EAAEMVLAAGETPEVAD 357

[Close]

BLAST of PI0021406 vs. NCBI nr
Match: KAG6577434.1 (Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015507.1 Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 610.9 bits (1574), Expect = 7.0e-171
Identity = 321/361 (88.92%), Postives = 342/361 (94.74%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPLASPPSLRLK-STAAASLSISASASQPPYDLNQ 60
           MASIAS+L  KL RPSLL +SSFHG+PL   PS RLK +TAAASLSISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPIKLHRPSLL-ESSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 360
           MGPTFGAMMISGQKAAHLALK+LG+TNSI DE+GE G R+V  E  E++LAAGE+PEVAD
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKTNSI-DEKGENGGREV--EAAEMVLAAGETPEVAD 357

[Close]

BLAST of PI0021406 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 527.7 bits (1358), Expect = 7.3e-150
Identity = 278/361 (77.01%), Postives = 307/361 (85.04%), Query Frame = 0


Query: 1   MASIASTLTTKLQRPSLLHDSSFHGTPL-ASPPSLRLKSTAAASLSISASASQPPYDLNQ 60
           MA+IASTL+    +P  L DSSFHG+ + A+P S+ LK       S S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR-----SFSVRATTAGYDLNA 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQ+ YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGETNSIGDEEGEMGERKVGLEEEELLLAAGESPEVAD 360
           MGPTFGAMMISGQKA  LALK+LG  N+I   +G +    VG    EL+LAA +S E  D
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAI---DGTL----VGNLSPELVLAAADSAETVD 349

The following BLAST results are available for this feature:

BLAST of PI0021406 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	6.0e-157	81.10	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
Q38709	6.4e-151	78.95	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
Q38814	1.0e-148	77.01	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]
F6H7K5	1.3e-148	77.13	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
F6H9A9	6.6e-148	74.86	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]

BLAST of PI0021406 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L546	1.0e-191	97.22	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A1S3CJ81	6.6e-191	97.50	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A6J1J7L4	1.1e-172	89.75	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1EVU2	2.1e-168	87.26	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1FWL6	7.8e-168	89.20	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of PI0021406 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152916.1	2.1e-191	97.22	thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothet...	[more]
XP_008463440.1	1.4e-190	97.50	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo]	[more]
XP_038905461.1	9.4e-184	95.05	thiamine thiazole synthase, chloroplastic [Benincasa hispida]	[more]
XP_022985241.1	2.2e-172	89.75	thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima]	[more]
KAG6577434.1	7.0e-171	88.92	Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]

BLAST of PI0021406 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	7.3e-150	77.01	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00411	PNDRDTASEI	coord: 345..359 score: 28.63 coord: 89..111 score: 30.31
None	No IPR available	GENE3D	6.10.250.2840		coord: 56..86 e-value: 2.8E-19 score: 70.4
None	No IPR available	PFAM	PF01946	Thi4	coord: 71..304 e-value: 2.2E-114 score: 380.6
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 1..360
None	No IPR available	PANTHER	PTHR43422:SF11	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 1..360
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 67..322 e-value: 2.9E-82 score: 273.7
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 87..322 e-value: 4.1E-122 score: 407.9
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 46..316
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..331 score: 68.59668

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0021406.1	PI0021406.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0052837	thiazole biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0016763	pentosyltransferase activity