PI0021203 (gene) Melon (PI 482460) v1

BLAST of PI0021203 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.3e-140
Identity = 258/450 (57.33%), Postives = 325/450 (72.22%), Query Frame = 0

Query: 28  DSHYLTVELTDLFPSASCT-RRSPQAYTSSLGEQSSLEVIHRHGPC---GDGVSKAPTEA 87
           DSH  T++++ L PS+S +   SP+A T+    +SSL V HRHG C    +G + +P   
Sbjct: 32  DSH--TIQVSSLLPSSSSSCVLSPRASTT----KSSLHVTHRHGTCSRLNNGKATSPDHV 91

Query: 88  ETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPAKSGATIGSGNYIVNVGLGTPKKY 147
           E L  DQARV+ IHSK + +L + D +  SK+T +PAK G+T+GSGNYIV VGLGTPK  
Sbjct: 92  EILRLDQARVNSIHSKLSKKL-ATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKND 151

Query: 148 LSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTTYSNISCSSPDCSQLESGTGNQP 207
           LSLIFDTGSDLTWTQCQPC R CY+QKEP+F+PS+ST+Y N+SCSS  C  L S TGN  
Sbjct: 152 LSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAG 211

Query: 208 GCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVIDNFLFGCGQNNRGLFGSAAGLIG 267
            CSA+  CIYGIQYGDQSFSVG+ AK+  TLT++DV D   FGCG+NN+GLF   AGL+G
Sbjct: 212 SCSASN-CIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLG 271

Query: 268 LGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFGGGGGGGALKYTPITKAHGVANFY 327
           LG+DK+S   QTA  Y ++FSYCLP ++S TG+LTFG  G   ++K+TPI+      +FY
Sbjct: 272 LGRDKLSFPSQTATAYNKIFSYCLPSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFY 331

Query: 328 GVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMAMYPKAP 387
           G++IV I VGG ++PI S+VFST GA+IDSGTVITRLPP AY+AL+S+F+  M+ YP   
Sbjct: 332 GLNIVAITVGGQKLPIPSTVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTS 391

Query: 388 ELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGIGTLYGGSTSQVCLAFAGNQDPST 447
            +SILDTC+DLS + T+ IP V F F GG  + L   G  Y    SQVCLAFAGN D S 
Sbjct: 392 GVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDSN 451

Query: 448 VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            AI GNVQQ+TL+VVYD  GG++GF  NGC
Sbjct: 452 AAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of PI0021203 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 5.1e-116
Identity = 230/473 (48.63%), Postives = 308/473 (65.12%), Query Frame = 0

Query: 7   IMLFFAFSFLFFQ----SFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQSS 66
           IML    ++ F +    S +GK+  DS+  T++++ LFPS+S    S +A  +    +SS
Sbjct: 12  IMLCVCLNWCFAEGAEKSDSGKVL-DSY--TIQVSSLFPSSSSCVPSSKASNT----KSS 71

Query: 67  LEVIHRHGPCGDGVSKAPTEAETLV-HDQARVDFIHSKFAAELESVDRLRPSKATKIPAK 126
           L V+H HG C    S A  + + ++  DQARV+ I+SK +    S + +  +K+T++PAK
Sbjct: 72  LRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSK--NSANEVSEAKSTELPAK 131

Query: 127 SGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTT 186
           SG T+GSGNYIV +G+GTPK  LSL+FDTGSDLTWTQC+PC   CY+QKEP F+PS S+T
Sbjct: 132 SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSST 191

Query: 187 YSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVID 246
           Y N+SCSSP C   ES       CSA+  C+Y I YGD+SF+ G+ AK+  TLT++DV++
Sbjct: 192 YQNVSCSSPMCEDAES-------CSASN-CVYSIVYGDKSFTQGFLAKEKFTLTNSDVLE 251

Query: 247 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPK-TSSSTGYLTFG 306
           +  FGCG+NN+GLF   AGL+GLG  K+S+  QT   Y  +FSYCLP  TS+STG+LTFG
Sbjct: 252 DVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFG 311

Query: 307 GGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITRL 366
             G   ++K+TPI+      N YG+DI+GI VG  ++ I+ + FST GAIIDSGTV TRL
Sbjct: 312 SAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSFSTEGAIIDSGTVFTRL 371

Query: 367 PPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGI 426
           P   Y+ L+S F++ M+ Y       + DTCYD +   T+  P + F F G   + L G 
Sbjct: 372 PTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGS 431

Query: 427 GTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G       SQVCLAFAGN D    AI GNVQQ TL VVYDV GG++GF  NGC
Sbjct: 432 GISLPIKISQVCLAFAGNDD--LPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of PI0021203 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 7.0e-73
Identity = 178/496 (35.89%), Postives = 262/496 (52.82%), Query Frame = 0

Query: 6   SIMLFFAFSFLFFQSFAG-----KISPDSHYLTVELTDLFPSASCTRRSPQAYTSSL--- 65
           +++    F FL   SF+       + P+SH L        P AS     P + + SL   
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSL--------PCASPVSFQPDSDSESLLES 66

Query: 66  -------GEQSSLEVIHRHGPCGDGVSKAPTE--AETLVHDQARVDFIHSKFAAEL--ES 125
                   E SS   ++         +K P E  +  L  D  RV  I +  AA++   +
Sbjct: 67  EFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSI-ATLAAQIPGRN 126

Query: 126 VDRLRPSKATKIPAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYC 185
           V              SG + GSG Y   +G+GTP +Y+ ++ DTGSD+ W QC PC R C
Sbjct: 127 VTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR-C 186

Query: 186 YNQKEPVFDPSQSTTYSNISCSSPDCSQLESGTGNQPGCSAAR-ACIYGIQYGDQSFSVG 245
           Y+Q +P+FDP +S TY+ I CSSP C +L+S      GC+  R  C+Y + YGD SF+VG
Sbjct: 187 YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSA-----GCNTRRKTCLYQVSYGDGSFTVG 246

Query: 246 YFAKDTLTLTSTDVIDNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSY 305
            F+ +TLT     V      GCG +N GLF  AAGL+GLG+ K+S   QT  ++ Q FSY
Sbjct: 247 DFSTETLTFRRNRV-KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 306

Query: 306 CLPKTSSST--GYLTFGGGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIP-ISSS 365
           CL   S+S+    + FG        ++TP+     +  FY V ++GI VGGT++P +++S
Sbjct: 307 CLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTAS 366

Query: 366 VF-----STSGAIIDSGTVITRLPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKY 425
           +F        G IIDSGT +TRL   AY A++ AF+ G     +AP+ S+ DTC+DLS  
Sbjct: 367 LFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNM 426

Query: 426 STIKIPVVVFVFKGGEELVLSGIGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQV 474
           + +K+P VV  F+G +  + +    +   +  + C AFAG      ++IIGN+QQ+  +V
Sbjct: 427 NEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTM--GGLSIIGNIQQQGFRV 484

BLAST of PI0021203 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.3e-68
Identity = 161/409 (39.36%), Postives = 225/409 (55.01%), Query Frame = 0

Query: 86  LVHDQARVDFIHSKFAAELESVDR--LRP---------SKATKIPAKSGATIGSGNYIVN 145
           L  D +RV  I +K    +E VDR  L+P         ++    P  SGA+ GSG Y   
Sbjct: 106 LERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSR 165

Query: 146 VGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTTYSNISCSSPDCSQ 205
           +G+GTP K + L+ DTGSD+ W QC+PCA  CY Q +PVF+P+ S+TY +++CS+P CS 
Sbjct: 166 IGVGTPAKEMYLVLDTGSDVNWIQCEPCAD-CYQQSDPVFNPTSSSTYKSLTCSAPQCSL 225

Query: 206 LESGTGNQPGCSAARA--CIYGIQYGDQSFSVGYFAKDTLTLTSTDVIDNFLFGCGQNNR 265
           LE+        SA R+  C+Y + YGD SF+VG  A DT+T  ++  I+N   GCG +N 
Sbjct: 226 LET--------SACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNE 285

Query: 266 GLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCL-PKTSSSTGYLTFGGGGGGGALKYT 325
           GLF  AAGL+GLG   +SI   T Q     FSYCL  + S  +  L F     GG     
Sbjct: 286 GLFTGAAGLLGLGGGVLSI---TNQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATA 345

Query: 326 PITKAHGVANFYGVDIVGIKVGGTQIPISSSVF-----STSGAIIDSGTVITRLPPDAYS 385
           P+ +   +  FY V + G  VGG ++ +  ++F      + G I+D GT +TRL   AY+
Sbjct: 346 PLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYN 405

Query: 386 ALKSAFQKGMAMYPK-APELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGIGTLYG 445
           +L+ AF K      K +  +S+ DTCYD S  ST+K+P V F F GG+ L L     L  
Sbjct: 406 SLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIP 465

Query: 446 -GSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
              +   C AFA     S+++IIGNVQQ+  ++ YD+    IG   N C
Sbjct: 466 VDDSGTFCFAFAPTS--SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of PI0021203 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 4.4e-67
Identity = 142/359 (39.55%), Postives = 206/359 (57.38%), Query Frame = 0

Query: 122 SGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTT 181
           SG   GSG Y V +G+G+P +   ++ D+GSD+ W QCQPC + CY Q +PVFDP++S +
Sbjct: 122 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-KLCYKQSDPVFDPAKSGS 181

Query: 182 YSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVID 241
           Y+ +SC S  C ++E+      GC +   C Y + YGD S++ G  A +TLT   T V+ 
Sbjct: 182 YTGVSCGSSVCDRIENS-----GCHSG-GCRYEVMYGDGSYTKGTLALETLTFAKT-VVR 241

Query: 242 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCL-PKTSSSTGYLTFG 301
           N   GCG  NRG+F  AAGL+G+G   +S V Q + + G  F YCL  + + STG L FG
Sbjct: 242 NVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG 301

Query: 302 GGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTS-----GAIIDSGT 361
                    + P+ +     +FY V + G+ VGG +IP+   VF  +     G ++D+GT
Sbjct: 302 REALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGT 361

Query: 362 VITRLPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEEL 421
            +TRLP  AY A +  F+   A  P+A  +SI DTCYDLS + ++++P V F F  G  L
Sbjct: 362 AVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVL 421

Query: 422 VLSGIGTLYG-GSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            L     L     +   C AFA +  P+ ++IIGN+QQ+ +QV +D   G +GFG N C
Sbjct: 422 TLPARNFLMPVDDSGTYCFAFAAS--PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of PI0021203 vs. ExPASy TrEMBL
Match: A0A1S3BVJ7 (aspartyl protease family protein At5g10770-like OS=Cucumis melo OX=3656 GN=LOC103493725 PE=4 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.2e-248
Identity = 439/475 (92.42%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MASLSSIML-FFAFSFLFFQSFAGK-ISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLG 60
           MASL SI+L FFAFSFLFFQSFAGK +SPDSHYLTVEL DLFPSASCTRRSPQA+TSS+G
Sbjct: 1   MASLPSIILFFFAFSFLFFQSFAGKLLSPDSHYLTVELADLFPSASCTRRSPQAHTSSVG 60

Query: 61  EQSSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKI 120
           EQSSLEVIHRHGPCGD VS APT AE  V DQARVDFIHSKFA ELESVDRLRPSKATKI
Sbjct: 61  EQSSLEVIHRHGPCGDDVSNAPTAAEMFVQDQARVDFIHSKFAGELESVDRLRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQ 180
           PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQK+PVF PSQ
Sbjct: 121 PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKDPVFAPSQ 180

Query: 181 STTYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTD 240
           STTYSNISCSS DCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAK+TLTLTS D
Sbjct: 181 STTYSNISCSSSDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKETLTLTSND 240

Query: 241 VIDNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLT 300
           VI+NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYLT
Sbjct: 241 VIENFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLT 300

Query: 301 FGGGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVIT 360
           FGGGGGGGALKYTPITKAHGVANFYG+DIVGIKVGGTQIPISSSVFSTSGAIIDSGTVIT
Sbjct: 301 FGGGGGGGALKYTPITKAHGVANFYGLDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVIT 360

Query: 361 RLPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLS 420
           RLPPDAYSALKSAFQKGMA YPKAPELSILDTCYDLSKYSTI+IP V  +FKG EEL L 
Sbjct: 361 RLPPDAYSALKSAFQKGMARYPKAPELSILDTCYDLSKYSTIQIPKVGIMFKGQEELDLD 420

Query: 421 GIGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G G +YG STSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GTGIMYGASTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 475

BLAST of PI0021203 vs. ExPASy TrEMBL
Match: A0A6J1F908 (aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443184 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.5e-230
Identity = 406/474 (85.65%), Postives = 430/474 (90.72%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MA LSS MLFFAF FLFF S AGK+SPD+H LTVEL DL PSA+C RRS QA  S    Q
Sbjct: 1   MAPLSS-MLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQ 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCG G SKAPT AE  V DQARVDFIHSKF+ + +S+DRLRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC+PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSP CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLT TDVI
Sbjct: 181 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
            NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYL FG
Sbjct: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFG 300

Query: 301 G-GGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITR 360
           G GGGGGALKYTPITKAHGVANFYGVDIVG+KVGG QIPIS+SVFSTSGAIIDSGTVITR
Sbjct: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITR 360

Query: 361 LPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSG 420
           LPPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY++++IP V F+FKGG  L L G
Sbjct: 361 LPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDG 420

Query: 421 IGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            G LYG ST+QVCLAFAGN DPS+VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 TGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of PI0021203 vs. ExPASy TrEMBL
Match: A0A6J1IL31 (aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477209 PE=3 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 6.1e-229
Identity = 404/474 (85.23%), Postives = 428/474 (90.30%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MA LSS ML FAF F FF S AGK+SPD+H LTVEL DL PSA+C RRS QA  S    Q
Sbjct: 1   MAPLSS-MLLFAFCFFFFHSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQISGGRTQ 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCGDG SKAPT AE  V DQARVDFIHSKF+ + +S+DRLRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGDGNSKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCAPCARYCYSQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSP CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLT TDVI
Sbjct: 181 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
            NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYL FG
Sbjct: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFG 300

Query: 301 G-GGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITR 360
           G GGGGGALKYTPITKAHGVANFYGVDIVG+KVGG QIPIS+SVFSTSGAIIDSGTVITR
Sbjct: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITR 360

Query: 361 LPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSG 420
           LPPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY++++IP V F+FKGG  L L G
Sbjct: 361 LPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDG 420

Query: 421 IGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            G LYG ST+QVCLAFAGN DPS+VAIIGNVQQKT+QVVYDVGGGKIGFGYNGC
Sbjct: 421 TGILYGASTTQVCLAFAGNLDPSSVAIIGNVQQKTMQVVYDVGGGKIGFGYNGC 473

BLAST of PI0021203 vs. ExPASy TrEMBL
Match: A0A6J1FDU3 (aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443184 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.0e-228
Identity = 404/474 (85.23%), Postives = 429/474 (90.51%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MA LSS MLFFAF FLFF S AGK+SPD+H LTVEL DL PSA+C RRS Q   +    Q
Sbjct: 1   MAPLSS-MLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQGRRT----Q 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCG G SKAPT AE  V DQARVDFIHSKF+ + +S+DRLRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC+PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSP CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLT TDVI
Sbjct: 181 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
            NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYL FG
Sbjct: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFG 300

Query: 301 G-GGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITR 360
           G GGGGGALKYTPITKAHGVANFYGVDIVG+KVGG QIPIS+SVFSTSGAIIDSGTVITR
Sbjct: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITR 360

Query: 361 LPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSG 420
           LPPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY++++IP V F+FKGG  L L G
Sbjct: 361 LPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDG 420

Query: 421 IGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            G LYG ST+QVCLAFAGN DPS+VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 TGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 469

BLAST of PI0021203 vs. ExPASy TrEMBL
Match: A0A6J1III3 (aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477209 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.7e-227
Identity = 402/474 (84.81%), Postives = 427/474 (90.08%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MA LSS ML FAF F FF S AGK+SPD+H LTVEL DL PSA+C RRS Q   +    Q
Sbjct: 1   MAPLSS-MLLFAFCFFFFHSSAGKVSPDAHRLTVELADLLPSAACNRRSVQGGRT----Q 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCGDG SKAPT AE  V DQARVDFIHSKF+ + +S+DRLRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGDGNSKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCAPCARYCYSQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSP CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLT TDVI
Sbjct: 181 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
            NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYL FG
Sbjct: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFG 300

Query: 301 G-GGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITR 360
           G GGGGGALKYTPITKAHGVANFYGVDIVG+KVGG QIPIS+SVFSTSGAIIDSGTVITR
Sbjct: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITR 360

Query: 361 LPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSG 420
           LPPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY++++IP V F+FKGG  L L G
Sbjct: 361 LPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDG 420

Query: 421 IGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            G LYG ST+QVCLAFAGN DPS+VAIIGNVQQKT+QVVYDVGGGKIGFGYNGC
Sbjct: 421 TGILYGASTTQVCLAFAGNLDPSSVAIIGNVQQKTMQVVYDVGGGKIGFGYNGC 469

BLAST of PI0021203 vs. NCBI nr
Match: XP_004150866.1 (aspartyl protease family protein At5g10770 [Cucumis sativus] >KAE8649932.1 hypothetical protein Csa_012305 [Cucumis sativus])

HSP 1 Score: 875.5 bits (2261), Expect = 2.0e-250
Identity = 439/473 (92.81%), Postives = 453/473 (95.77%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MASLSSIMLFFAFS LFFQ+FAGK+SPDSH+LTV+L  LFPSASCTRRSPQ +TSSLGEQ
Sbjct: 1   MASLSSIMLFFAFSSLFFQAFAGKLSPDSHFLTVDLAGLFPSASCTRRSPQVHTSSLGEQ 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCGD VS APT AE LV DQ+RVDFIHSK A ELESVDRLR SKATKIPA
Sbjct: 61  SSLEVIHRHGPCGDEVSNAPTAAEMLVKDQSRVDFIHSKIAGELESVDRLRGSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIV+VGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVSVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKDPVFVPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAK+TLTLTSTDVI
Sbjct: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKETLTLTSTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
           +NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG
Sbjct: 241 ENFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300

Query: 301 GGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITRL 360
           GGGGGGALKYTPITKAHGVANFYGVDIVG+KVGGTQIPISSSVFSTSGAIIDSGTVITRL
Sbjct: 301 GGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGTQIPISSSVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGI 420
           PPDAYSALKSAF+KGMA YPKAPELSILDTCYDLSKYSTI+IP V FVFKGGEEL L GI
Sbjct: 361 PPDAYSALKSAFEKGMAKYPKAPELSILDTCYDLSKYSTIQIPKVGFVFKGGEELDLDGI 420

Query: 421 GTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G +YG STSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GIMYGASTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of PI0021203 vs. NCBI nr
Match: XP_008452815.1 (PREDICTED: aspartyl protease family protein At5g10770-like [Cucumis melo])

HSP 1 Score: 868.6 bits (2243), Expect = 2.4e-248
Identity = 439/475 (92.42%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MASLSSIML-FFAFSFLFFQSFAGK-ISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLG 60
           MASL SI+L FFAFSFLFFQSFAGK +SPDSHYLTVEL DLFPSASCTRRSPQA+TSS+G
Sbjct: 1   MASLPSIILFFFAFSFLFFQSFAGKLLSPDSHYLTVELADLFPSASCTRRSPQAHTSSVG 60

Query: 61  EQSSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKI 120
           EQSSLEVIHRHGPCGD VS APT AE  V DQARVDFIHSKFA ELESVDRLRPSKATKI
Sbjct: 61  EQSSLEVIHRHGPCGDDVSNAPTAAEMFVQDQARVDFIHSKFAGELESVDRLRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQ 180
           PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQK+PVF PSQ
Sbjct: 121 PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKDPVFAPSQ 180

Query: 181 STTYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTD 240
           STTYSNISCSS DCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAK+TLTLTS D
Sbjct: 181 STTYSNISCSSSDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKETLTLTSND 240

Query: 241 VIDNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLT 300
           VI+NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYLT
Sbjct: 241 VIENFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLT 300

Query: 301 FGGGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVIT 360
           FGGGGGGGALKYTPITKAHGVANFYG+DIVGIKVGGTQIPISSSVFSTSGAIIDSGTVIT
Sbjct: 301 FGGGGGGGALKYTPITKAHGVANFYGLDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVIT 360

Query: 361 RLPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLS 420
           RLPPDAYSALKSAFQKGMA YPKAPELSILDTCYDLSKYSTI+IP V  +FKG EEL L 
Sbjct: 361 RLPPDAYSALKSAFQKGMARYPKAPELSILDTCYDLSKYSTIQIPKVGIMFKGQEELDLD 420

Query: 421 GIGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G G +YG STSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GTGIMYGASTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 475

BLAST of PI0021203 vs. NCBI nr
Match: XP_038897918.1 (aspartyl protease family protein At5g10770-like [Benincasa hispida])

HSP 1 Score: 831.6 bits (2147), Expect = 3.3e-237
Identity = 413/473 (87.32%), Postives = 441/473 (93.23%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MASLSS MLFFAFSFLFFQSFAGKISPDSH+LTVEL  LFPSASCTRRSPQA+ SS  EQ
Sbjct: 1   MASLSS-MLFFAFSFLFFQSFAGKISPDSHHLTVELAGLFPSASCTRRSPQAHISSQREQ 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCG G SK+PT AE  V DQARVDFI+SKF+ + +++D LRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGVGDSKSPTAAEMFVQDQARVDFINSKFSGKFKAMDSLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQC+PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCEPCARYCYDQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSPDC+QLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLTSTDVI
Sbjct: 181 TYSNITCSSPDCAQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTSTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
           DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYLTFG
Sbjct: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLTFG 300

Query: 301 GGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITRL 360
            GGGGGALKYTPI +AHGVANFYG++IVG+KVGGTQIPIS SVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPIIEAHGVANFYGLNIVGMKVGGTQIPISPSVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGI 420
           PPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY+ + IP V F+FKGG E+ L G 
Sbjct: 361 PPDAYSALKSAFQKGMVRYPKAPELSILDTCYDLSKYAAVSIPKVSFLFKGGTEIDLDGT 420

Query: 421 GTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G LYG ST QVCLAFAGN+DPST+AIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTDQVCLAFAGNRDPSTIAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 472

BLAST of PI0021203 vs. NCBI nr
Match: XP_022936652.1 (aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita moschata] >KAG6591512.1 Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.7 bits (2085), Expect = 5.1e-230
Identity = 406/474 (85.65%), Postives = 430/474 (90.72%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MA LSS MLFFAF FLFF S AGK+SPD+H LTVEL DL PSA+C RRS QA  S    Q
Sbjct: 1   MAPLSS-MLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQ 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCG G SKAPT AE  V DQARVDFIHSKF+ + +S+DRLRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC+PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSP CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLT TDVI
Sbjct: 181 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
            NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYL FG
Sbjct: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFG 300

Query: 301 G-GGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITR 360
           G GGGGGALKYTPITKAHGVANFYGVDIVG+KVGG QIPIS+SVFSTSGAIIDSGTVITR
Sbjct: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITR 360

Query: 361 LPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSG 420
           LPPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY++++IP V F+FKGG  L L G
Sbjct: 361 LPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDG 420

Query: 421 IGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            G LYG ST+QVCLAFAGN DPS+VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 TGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of PI0021203 vs. NCBI nr
Match: KAG7024399.1 (Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-229
Identity = 405/474 (85.44%), Postives = 429/474 (90.51%), Query Frame = 0

Query: 1   MASLSSIMLFFAFSFLFFQSFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQ 60
           MA LSS MLFFAF FLFF S AGK+SPD+H LTVEL DL PSA+C RRS QA  S    Q
Sbjct: 1   MAPLSS-MLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQ 60

Query: 61  SSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPA 120
           SSLEVIHRHGPCG G SKAPT  E  V DQARVDFIHSKF+ + +S+DRLRPSKATKIPA
Sbjct: 61  SSLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 120

Query: 121 KSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQST 180
           KSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC+PCARYCY+QK+PVF PSQST
Sbjct: 121 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQST 180

Query: 181 TYSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVI 240
           TYSNI+CSSP CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAK+TLTLT TDVI
Sbjct: 181 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240

Query: 241 DNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFG 300
            NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQ+FSYCLPKTSSSTGYL FG
Sbjct: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFG 300

Query: 301 G-GGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITR 360
           G GGGGGALKYTPITKAHGVANFYGVDIVG+KVGG QIPIS+SVFSTSGAIIDSGTVITR
Sbjct: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITR 360

Query: 361 LPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSG 420
           LPPDAYSALKSAFQKGM  YPKAPELSILDTCYDLSKY++++IP V F+FKGG  L L G
Sbjct: 361 LPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDG 420

Query: 421 IGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            G LYG ST+QVCLAFAGN DPS+VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 TGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of PI0021203 vs. TAIR 10
Match: AT5G10770.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 501.1 bits (1289), Expect = 9.6e-142
Identity = 258/450 (57.33%), Postives = 325/450 (72.22%), Query Frame = 0

Query: 28  DSHYLTVELTDLFPSASCT-RRSPQAYTSSLGEQSSLEVIHRHGPC---GDGVSKAPTEA 87
           DSH  T++++ L PS+S +   SP+A T+    +SSL V HRHG C    +G + +P   
Sbjct: 32  DSH--TIQVSSLLPSSSSSCVLSPRASTT----KSSLHVTHRHGTCSRLNNGKATSPDHV 91

Query: 88  ETLVHDQARVDFIHSKFAAELESVDRLRPSKATKIPAKSGATIGSGNYIVNVGLGTPKKY 147
           E L  DQARV+ IHSK + +L + D +  SK+T +PAK G+T+GSGNYIV VGLGTPK  
Sbjct: 92  EILRLDQARVNSIHSKLSKKL-ATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKND 151

Query: 148 LSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTTYSNISCSSPDCSQLESGTGNQP 207
           LSLIFDTGSDLTWTQCQPC R CY+QKEP+F+PS+ST+Y N+SCSS  C  L S TGN  
Sbjct: 152 LSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAG 211

Query: 208 GCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVIDNFLFGCGQNNRGLFGSAAGLIG 267
            CSA+  CIYGIQYGDQSFSVG+ AK+  TLT++DV D   FGCG+NN+GLF   AGL+G
Sbjct: 212 SCSASN-CIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLG 271

Query: 268 LGQDKISIVKQTAQKYGQVFSYCLPKTSSSTGYLTFGGGGGGGALKYTPITKAHGVANFY 327
           LG+DK+S   QTA  Y ++FSYCLP ++S TG+LTFG  G   ++K+TPI+      +FY
Sbjct: 272 LGRDKLSFPSQTATAYNKIFSYCLPSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFY 331

Query: 328 GVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMAMYPKAP 387
           G++IV I VGG ++PI S+VFST GA+IDSGTVITRLPP AY+AL+S+F+  M+ YP   
Sbjct: 332 GLNIVAITVGGQKLPIPSTVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTS 391

Query: 388 ELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGIGTLYGGSTSQVCLAFAGNQDPST 447
            +SILDTC+DLS + T+ IP V F F GG  + L   G  Y    SQVCLAFAGN D S 
Sbjct: 392 GVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDSN 451

Query: 448 VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            AI GNVQQ+TL+VVYD  GG++GF  NGC
Sbjct: 452 AAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of PI0021203 vs. TAIR 10
Match: AT5G10760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 419.5 bits (1077), Expect = 3.7e-117
Identity = 230/473 (48.63%), Postives = 308/473 (65.12%), Query Frame = 0

Query: 7   IMLFFAFSFLFFQ----SFAGKISPDSHYLTVELTDLFPSASCTRRSPQAYTSSLGEQSS 66
           IML    ++ F +    S +GK+  DS+  T++++ LFPS+S    S +A  +    +SS
Sbjct: 12  IMLCVCLNWCFAEGAEKSDSGKVL-DSY--TIQVSSLFPSSSSCVPSSKASNT----KSS 71

Query: 67  LEVIHRHGPCGDGVSKAPTEAETLV-HDQARVDFIHSKFAAELESVDRLRPSKATKIPAK 126
           L V+H HG C    S A  + + ++  DQARV+ I+SK +    S + +  +K+T++PAK
Sbjct: 72  LRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSK--NSANEVSEAKSTELPAK 131

Query: 127 SGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTT 186
           SG T+GSGNYIV +G+GTPK  LSL+FDTGSDLTWTQC+PC   CY+QKEP F+PS S+T
Sbjct: 132 SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSST 191

Query: 187 YSNISCSSPDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKDTLTLTSTDVID 246
           Y N+SCSSP C   ES       CSA+  C+Y I YGD+SF+ G+ AK+  TLT++DV++
Sbjct: 192 YQNVSCSSPMCEDAES-------CSASN-CVYSIVYGDKSFTQGFLAKEKFTLTNSDVLE 251

Query: 247 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCLPK-TSSSTGYLTFG 306
           +  FGCG+NN+GLF   AGL+GLG  K+S+  QT   Y  +FSYCLP  TS+STG+LTFG
Sbjct: 252 DVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFG 311

Query: 307 GGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVITRL 366
             G   ++K+TPI+      N YG+DI+GI VG  ++ I+ + FST GAIIDSGTV TRL
Sbjct: 312 SAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSFSTEGAIIDSGTVFTRL 371

Query: 367 PPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGI 426
           P   Y+ L+S F++ M+ Y       + DTCYD +   T+  P + F F G   + L G 
Sbjct: 372 PTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGS 431

Query: 427 GTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G       SQVCLAFAGN D    AI GNVQQ TL VVYDV GG++GF  NGC
Sbjct: 432 GISLPIKISQVCLAFAGNDD--LPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of PI0021203 vs. TAIR 10
Match: AT1G79720.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 295.8 bits (756), Expect = 6.1e-80
Identity = 176/440 (40.00%), Postives = 246/440 (55.91%), Query Frame = 0

Query: 49  SPQAYTSSLG---EQSSLEVIHRHGPCGDGVSKAPTEAETLVHDQARVDFIHSKFAAELE 108
           S   ++ SLG   E ++LE+ HR    G  +         LV D  RV  +  K  A   
Sbjct: 51  STSCFSRSLGKGRESTTLEMKHRELCSGKTIDLGKKMRRALVLDNIRVQSLQLKIKAMTS 110

Query: 109 SVDRLRPSKATKIPAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARY 168
           S      S+ T+IP  SG  + S NYIV V LG   K +SLI DTGSDLTW QCQPC R 
Sbjct: 111 STTEQSVSE-TQIPLTSGIKLESLNYIVTVELG--GKNMSLIVDTGSDLTWVQCQPC-RS 170

Query: 169 CYNQKEPVFDPSQSTTYSNISCSSPDCSQLESGTGNQPGCSAARA-----CIYGIQYGDQ 228
           CYNQ+ P++DPS S++Y  + C+S  C  L + T N   C          C Y + YGD 
Sbjct: 171 CYNQQGPLYDPSVSSSYKTVFCNSSTCQDLVAATSNSGPCGGNNGVVKTPCEYVVSYGDG 230

Query: 229 SFSVGYFAKDTLTLTSTDVIDNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYG 288
           S++ G  A +++ L  T  ++NF+FGCG+NN+GLFG ++GL+GLG+  +S+V QT + + 
Sbjct: 231 SYTRGDLASESILLGDTK-LENFVFGCGRNNKGLFGGSSGLMGLGRSSVSLVSQTLKTFN 290

Query: 289 QVFSYCLPK-TSSSTGYLTFGGGG----GGGALKYTPITKAHGVANFYGVDIVGIKVGGT 348
            VFSYCLP     ++G L+FG          ++ YTP+ +   + +FY +++ G  +GG 
Sbjct: 291 GVFSYCLPSLEDGASGSLSFGNDSSVYTNSTSVSYTPLVQNPQLRSFYILNLTGASIGGV 350

Query: 349 QIPISSSVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLS 408
           ++  SS      G +IDSGTVITRLPP  Y A+K  F K  + +P AP  SILDTC++L+
Sbjct: 351 ELKSSS---FGRGILIDSGTVITRLPPSIYKAVKIEFLKQFSGFPTAPGYSILDTCFNLT 410

Query: 409 KYSTIKIPVVVFVFKGGEELVLSGIGTLY--GGSTSQVCLAFAGNQDPSTVAIIGNVQQK 468
            Y  I IP++  +F+G  EL +   G  Y      S VCLA A     + V IIGN QQK
Sbjct: 411 SYEDISIPIIKMIFQGNAELEVDVTGVFYFVKPDASLVCLALASLSYENEVGIIGNYQQK 470

Query: 469 TLQVVYDVGGGKIGFGYNGC 474
             +V+YD    ++G     C
Sbjct: 471 NQRVIYDTTQERLGIVGENC 482

BLAST of PI0021203 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 276.2 bits (705), Expect = 5.0e-74
Identity = 178/496 (35.89%), Postives = 262/496 (52.82%), Query Frame = 0

Query: 6   SIMLFFAFSFLFFQSFAG-----KISPDSHYLTVELTDLFPSASCTRRSPQAYTSSL--- 65
           +++    F FL   SF+       + P+SH L        P AS     P + + SL   
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSL--------PCASPVSFQPDSDSESLLES 66

Query: 66  -------GEQSSLEVIHRHGPCGDGVSKAPTE--AETLVHDQARVDFIHSKFAAEL--ES 125
                   E SS   ++         +K P E  +  L  D  RV  I +  AA++   +
Sbjct: 67  EFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSI-ATLAAQIPGRN 126

Query: 126 VDRLRPSKATKIPAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYC 185
           V              SG + GSG Y   +G+GTP +Y+ ++ DTGSD+ W QC PC R C
Sbjct: 127 VTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR-C 186

Query: 186 YNQKEPVFDPSQSTTYSNISCSSPDCSQLESGTGNQPGCSAAR-ACIYGIQYGDQSFSVG 245
           Y+Q +P+FDP +S TY+ I CSSP C +L+S      GC+  R  C+Y + YGD SF+VG
Sbjct: 187 YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSA-----GCNTRRKTCLYQVSYGDGSFTVG 246

Query: 246 YFAKDTLTLTSTDVIDNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSY 305
            F+ +TLT     V      GCG +N GLF  AAGL+GLG+ K+S   QT  ++ Q FSY
Sbjct: 247 DFSTETLTFRRNRV-KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 306

Query: 306 CLPKTSSST--GYLTFGGGGGGGALKYTPITKAHGVANFYGVDIVGIKVGGTQIP-ISSS 365
           CL   S+S+    + FG        ++TP+     +  FY V ++GI VGGT++P +++S
Sbjct: 307 CLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTAS 366

Query: 366 VF-----STSGAIIDSGTVITRLPPDAYSALKSAFQKGMAMYPKAPELSILDTCYDLSKY 425
           +F        G IIDSGT +TRL   AY A++ AF+ G     +AP+ S+ DTC+DLS  
Sbjct: 367 LFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNM 426

Query: 426 STIKIPVVVFVFKGGEELVLSGIGTLYGGSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQV 474
           + +K+P VV  F+G +  + +    +   +  + C AFAG      ++IIGN+QQ+  +V
Sbjct: 427 NEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTM--GGLSIIGNIQQQGFRV 484

BLAST of PI0021203 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-69
Identity = 161/409 (39.36%), Postives = 225/409 (55.01%), Query Frame = 0

Query: 86  LVHDQARVDFIHSKFAAELESVDR--LRP---------SKATKIPAKSGATIGSGNYIVN 145
           L  D +RV  I +K    +E VDR  L+P         ++    P  SGA+ GSG Y   
Sbjct: 106 LERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSR 165

Query: 146 VGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKEPVFDPSQSTTYSNISCSSPDCSQ 205
           +G+GTP K + L+ DTGSD+ W QC+PCA  CY Q +PVF+P+ S+TY +++CS+P CS 
Sbjct: 166 IGVGTPAKEMYLVLDTGSDVNWIQCEPCAD-CYQQSDPVFNPTSSSTYKSLTCSAPQCSL 225

Query: 206 LESGTGNQPGCSAARA--CIYGIQYGDQSFSVGYFAKDTLTLTSTDVIDNFLFGCGQNNR 265
           LE+        SA R+  C+Y + YGD SF+VG  A DT+T  ++  I+N   GCG +N 
Sbjct: 226 LET--------SACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNE 285

Query: 266 GLFGSAAGLIGLGQDKISIVKQTAQKYGQVFSYCL-PKTSSSTGYLTFGGGGGGGALKYT 325
           GLF  AAGL+GLG   +SI   T Q     FSYCL  + S  +  L F     GG     
Sbjct: 286 GLFTGAAGLLGLGGGVLSI---TNQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATA 345

Query: 326 PITKAHGVANFYGVDIVGIKVGGTQIPISSSVF-----STSGAIIDSGTVITRLPPDAYS 385
           P+ +   +  FY V + G  VGG ++ +  ++F      + G I+D GT +TRL   AY+
Sbjct: 346 PLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYN 405

Query: 386 ALKSAFQKGMAMYPK-APELSILDTCYDLSKYSTIKIPVVVFVFKGGEELVLSGIGTLYG 445
           +L+ AF K      K +  +S+ DTCYD S  ST+K+P V F F GG+ L L     L  
Sbjct: 406 SLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIP 465

Query: 446 -GSTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
              +   C AFA     S+++IIGNVQQ+  ++ YD+    IG   N C
Sbjct: 466 VDDSGTFCFAFAPTS--SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500