PI0021102 (gene) Melon (PI 482460) v1

Overview
NamePI0021102
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAlpha-amylase
Locationchr07: 24281999 .. 24290249 (+)
RNA-Seq ExpressionPI0021102
SyntenyPI0021102
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTGTAAAATAAAATTATGGACAAAATAGAATAAAATACTTAGTAAGAAAAGAAACAAACAAAAAATAGATTTAAACCAATAAAATAAATTAAGAGCAATATCGTGATTCACCAATTAGCACACGTTCCATCGGACGTCCCAGTGTTAGTGTTCCATGAAACTGACTGTTGCTTAACGCTTAAGCCACTGCAAAATGGTTAGCGACGGAAGTCCACATCCTGCACCCCTTCGATTGGTTCTAGCAAAGAATCTCGAGCCCACGCGGCTGCAGCTCAATTGCCTGATTGCAAATGGTCGACCAGATAAAATCGCTTCCCTGAACCAGGAGAACCAGGAACGTCGAACCGCTAAGTGATTGCTTCTTGGTAACGTTTTTCATTATTTGCTAACTTTTATTCATGATCGCCTTATGTTCTTGCGATGTTCAAATGCGGAATTCTAATCGCATGGCTTAGCGAAGCGTTTTGGTTGTTTAGCTATTTGCCATCAAATATGTATACACAACGAATCACTCTCAATCGTTGTAATTGCGTTTTTCTTTTATTCCTTCTGAATTGAAGATTTTTGCTAGCTGTTCTTATCTCTTTCTTGATTTTTTCAGCGGTAAATGTCTTCACGCCAATCTGAAACTGTGATTTCAGCTGATAAATTCGTATTGTCAAGGTTGTGAACGCATCTGCTGCATAGCTATTTAGAAGTTGATTTTAGAAAATTTAAAGAAGTGTTAGTGGTTTATCCAGTTTGAAATTGAGGAATAGATTCTCAAAATGAAGCTAGAGCCATGAATTGTCCCCACCACGTCGCTAAGTGTTCTGTTATAATTCTAGCTCGTTACCTTATTTTCTTTTCCGTTGTTCGAGTGGCCATCTGGATAATGAAGTTAAGACCACACAATATGAATGAGGTTACACTTTGCAGATTGTGATGCTTTTTTATTTTTGTTGTAGGACTAGGACGTCCCTATCTTCTGCAACCAATACGACAAGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAATGTATGCTTCTTGTTTAATTTGGCAATTGTTTCCTTGATGAGTTTTCTGCATCAGTGTGATATGCTTTTAGTTATAAATTTGTTAGAAATTAGAATATACATTATGATATACAAATTACTTATTTATGTTGGAATTCAGGTTTTTGATGAAAGCTACGAACAGACTAATACTGGTATGGTTTTTTCTTTGTTTTGACATTAAATTTAACATCATGATATGCTCACGTCAATCTTAAGTGTTCGTCAAAAGATCATACCTCTCTTGGAAGTGACTTCAAATTCTAATACATTTGCTTGGGTCTAACTAACCAAAGAGAATGCTTGATGATGCTTAAAATTTGACTCATGGATAAGAAATTTGAAAAGAAATATATGCTTCTTAGGTGTGAGTACGTTGCAATATTTGCTTTTAATTTAATAAAATTTGTGCTTTATGAAAAGGATCGATACACGTGGGCTTGAAATCAGAGCTGGTTTTCTGCTATGTAAATTTTCTTTTGATTGAAATTATCTTATCATTTTAATAATTTCAGTGTTTCTTTATGGTCACATGTAGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGTAGAGGTTACAATTTCAGCTTTCTGATTTTTCTCTAGAGCAGAGGTCAGCAGGAAAATATTATGTTGCTCTTAATTGAGGCGAAGTCTCGGAAACAAGAAAAGAGAAAAAATGTTTGCCGTTTGAAATTTATCCTCAATCTCGTTGATTATTTTATTGTAAATCGGTTAGAATTTGAGGTGGAAGGACTTCGGGACTGTGAATGTTTTAGATCAAATCTCCAAGTCGTGTTAAACTTATGAGAGAAATTTTGCACCCATAATATCCATTGTAATTACAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTGGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCGCCTCCAACTCATTCCATTGCTCCTCAAGGTCAAAGGAACAAATTCACTTGAATTTTTATGTTCCTTTCACATATATGCATACATAACTCTTGATATTTTCTTTACAATTTCAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCAGAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGTACTGTTGTAGGACATGGAGGAATGCATAATCGCTTCGATGGAATACTATTACCATGGAATGAGTATGCTGTTACCTCGTGTACTGGTGGCCTGGTATTTTTCTGTTACAAATTCCAAGTGAGCTCATAATTATCAGAAAACCTTTGGTCTTTAGTCTGTGGTATCTGTTCTAGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGGTAATATTGTCCTTCTCTGTTTGTGTGCGTGTGTATTTATATTGTGGGGAGGCTGGGGCGTTACAGAGGTTCAAGGATAATTGTTCTGAAAATGACAAGATCCCTCCTCTGTTTCAAAATTTCACTGTATCATCTTCTCAACGTTCGCCTTGTCTTTTCTTGTAAGGAGTTCTTCAATGGATTATAACGTATATTTGCCTTCTGCTTTTATTTTTGATGTTTTAACTCAGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAGTATTGGGATTCTTGTAATTATACTGGCCATGACTTGGACTATAATCAAGGTAACATGATGATGCATCATGTTGATAAATTGAAAACATGTTTTCAAGTTTCTGGATTTTTAGTTTTTTTTAATGGTTTTGTCTGTGAATTCCTATGTTCGTCTTATCAATTCATGTGTTCCATAGTGATGATTACAACTCTAAATGCAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCTGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGTATTGGTGTTGTGTGAGGCCATTGCTAACTCCCTTCCCTCCTCCAAATTGTAATACTACGGACCTCAAATCTTAATTACTTAAAATAGCAAGGATGAAGTGACAGCTATGTTATGGTTTATTTATTTCCCTCTAGATCAGTAGATCTACAATGTTTGACTTTTCACAACTCTTTCCATTTGCTTCTTTTCTCCTCCTTTATTGAGTGAAGTTTGGGTTTTGTTAATATTTGCTCTTGCTAGTACAATGCTTGACTAACATGCATTTGTTTCCTTGTTGGCTTCGTAATAAACATAATATAATTAATTTTGCTTGTAGGAAGCTGTTAAAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATTGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGTCGCATCTTGAAATCTATTTGTGAAATTATTGGTTGACAAATTTCAACATTTGTTTTCATTGCTCATTGTGCATGTGCGTTATAAAATGGTCTCAACTTGACCACGTGTCCATGTCCTTTTATTTGGATTAATATTTGAAAACTCATGTTCGGAAGTCATGCCCTGTATCGGGGATTGAGTGTGACCAGACCATACATGAAAATGTCATAGGAAGCAAGGGAGAAAAGAAGTATTGATGCATACCTGAATGAAGTTGTAATGGCTGGTTGTTCTACAGCTTCTATATGGCCAAATATGATGTCAACACAATAGCTACTGGACTCTGTTCCATTTGCCTATGTTGGTATTATCTTTTGTAAGATGCGTATGCGTTTTTGCAACATGTAACTGAGATTTATTGGCCAAGCTTTCTTTTATATAGGTTTGTTCAAATTGAACTAATTAAAATGTAAATAGTTCTAGGCTTCTAGCAATCCAACTCTGCATCTTAGTAAGTAGATAGCTTTAGATTGTTATTTGGAAACTCATAATTAATTTTTTTTCTTTGGTTGAAAGCAAGCGTCAAGTGTGCTTTCAAATATAGATATTGACTTGGCAGTTCATGACAATTAATGCAACTCGACATTCTTGGCAAATGAACTATATAATTCTTCATATGTATTTTCTTGAAACGTTTATAGAAGCTGAAAATGTATCATTAGAATGTTGGTGACTCCACTAACCTCAGCATACCATTGTTCTTGCAGACCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGTATACAAGTGTGGCATATCATTCCATTTGCAATCTCTTTATTGCTTGTACTCTTATTCTTGGTGTGCTGTGGCTTCTTCGTGGTTTTTGGAAGCTCTCAATTGTTTGGTCCAATTATTCGTTGTGTAAAGTCTAATTACCCCCCATCAGATGGTTCTCATTGTTCGTTGTGTGTTTTGGAAGCTTTCAATTGTTTGTTCCAATTGATCCTATGTACGACTCTCTGTCTACATTTGAAGTTAGTTTCCTACTTTTAGCTGCAAATTCTATGGCTATAACTGTTCAGTATACATTCAAAAAAAAAAAAAAAACTGTTCAGCATACTAAGCGATCAATCTTTGATGTTTATGGGTGAATTTCATTGAAAGAGCTTTATAATTATTTTCTAGTCTTACAGTTGTTACCGCAACTACATTTATGCTTGGCCTAGAGTCGTCACTTGTCTTCCAGTAAAAAAAAATTCCATCATGTATCTTTTTTCTGATTCCTGGAGCTTTGGTTATTTCCTGCAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTTTATGATTGGGGTGACTCAGTGCATGACGAGATAGTAAAATTAGTATGTTGCATAGTCAATAATGCTTCAATAGAGTTTGTTATCTTCTATTCAAGCAAACTTCTCATGGTACTTCAATTCTATTTTTCTTGCAGATAGGCATTCGAAAATGTCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTGGTATGGAATGGGCTCTTGCAACCTCTGGCCGCAGATATGCAGTGTGGCAGAAATAAGGTTTGGAAAATTTACCTGCTTTCCCCAACCTTATATGTTTCTTTTTGTGCCATCGGGGACTACGTGTGTAAACTATAAATTTTCATAACAAGTTGGCTACTCAAAGTATAGTATCATAAATAAAATCACTGTGGATGAAATGTATTTTCACATAGTCTAGATTAAATCACATCGCTTATATTACTTCTTTTGTGTAATTTTAAATAAACCGTATGGTTGAGTCTGTTAATAGAAATGATTGTTTTCTTCAAGCAAACTAACCTTCCCCATTTGGAGCTTTGAGTGCTATATATATGGTGGAATGACTCTCGAACAAAGTTTGATGTATTTGGATAGTTGGAACTTGGTAGATAGTTATTCATAATTTGAATTATGTAGATACATAATATGTTCTTATTTACGGATTCATCTGTGCTTTTAATTAAGTTCAAGTAAATGGTAAAATGGTGCTCATTATGAAATTGTTCAATAACGTTACTAAAGCTCGTTGTCATGGAGATGTTTACTACTTGGAGTACAATGATTTATAAACTTAGAATTGGAATATAATGAAAAGGAGGCACTTTGAAGTCCTCTACGAGATTGGGATGGTTTGTGGAATTGAGCTATGAAGAAATTTGAGGAGTTACACATCCTTAGAAAGGTTGTCTTATTCTTGTTCGGGAACTACAATCCGAAATTTTCTTTTTTTATTTTCTAAACCAAATATAGTCAAATCATATTCAAATAATTTGTTTATAAAAAAATTGTCCTAAATTACAAACATGTAAAGTAATGTTTGAAACTTTTAATGAGTACAATATAATCTCTATGGGCCATTCTATCATAAATTTTGAATTGAAGTAGAATACCAGGGTATTGAGCACTTGGTGTCGTTTCATCGATACCTCAAAACCAGTTATTACACTCCACCCAGTCCTTATTAGGGTGCATTTCTTATCATATGTAGGACTTCCCTTCATCAAGTTTCAATTTACTATGCTATATAGACACTGATGTCTTTATGTGGCCGTAAGGCTGAGCAATCATCGATTCTTTGGATAAATCTTATGGCATGGAGAAAGAAAGAAGTTAATTTGAATGTGCGAACCTCTGTGAATCTTGCATAGGGGGTCCACTGTATTGGAACTTTAAATTAAATTTGGTATGTATATTGATTGGAAAAGTAGGGAAAGAGTTTAGGCATAGCAAGCTGAAAAATCAACCGTGGACATGGTTTGATCGGCTCAGTTTTATTTAGTAGGTCGATTCAATTCAATTTCAGTTTGTTTGATAGTTGGTTATGATATATCTGATACTCCAAAAGGTTAGTCTCGGTATCTGACTAAATGGAGTCGAACTAATCTGTTTATTCTCTTAATTCTTAAAGTGTGTGCCACATGGCAGAAGAGCAGGGAAGAGACAACAAAGCATCGCCATCACAATGCCACGGTCAATTCCCTCATCATTTTTGCTTTGAAACTCCAAAAGATAAACAGAACAACGCAACCCTTCCACGGCTTCCCTTAATCTCAACCTTCATCCTGTATATGTATGTATTGTTGGGCCATTATCATGTTTATCAACAGCGAATCTCTATAATTTCTTGAGTTATGTAACCTTGTCCTGTGTGGACACTACCTTGTCCCAGTCCTCAAACTTGGACAACTTTGTTTTACGTGCCACTTGATTATTCGATAGGCTGATTAATTTATTCTCAAGGGATACTAATTAATCACAGTAGCTAGTTTGCTTTTATA

mRNA sequence

GTTTTTGTAAAATAAAATTATGGACAAAATAGAATAAAATACTTAGTAAGAAAAGAAACAAACAAAAAATAGATTTAAACCAATAAAATAAATTAAGAGCAATATCGTGATTCACCAATTAGCACACGTTCCATCGGACGTCCCAGTGTTAGTGTTCCATGAAACTGACTGTTGCTTAACGCTTAAGCCACTGCAAAATGGTTAGCGACGGAAGTCCACATCCTGCACCCCTTCGATTGGTTCTAGCAAAGAATCTCGAGCCCACGCGGCTGCAGCTCAATTGCCTGATTGCAAATGGTCGACCAGATAAAATCGCTTCCCTGAACCAGGAGAACCAGGAACGTCGAACCGCTAAGTGATTGCTTCTTGGACTAGGACGTCCCTATCTTCTGCAACCAATACGACAAGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAATGTTTTTGATGAAAGCTACGAACAGACTAATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTGGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCGCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCAGAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGTACTGTTGTAGGACATGGAGGAATGCATAATCGCTTCGATGGAATACTATTACCATGGAATGAGTATGCTGTTACCTCGTGTACTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAGTATTGGGATTCTTGTAATTATACTGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCTGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAAAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATTGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGACCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTTTATGATTGGGGTGACTCAGTGCATGACGAGATAGTAAAATTAATAGGCATTCGAAAATGTCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTGGTATGGAATGGGCTCTTGCAACCTCTGGCCGCAGATATGCAGTGTGGCAGAAATAAGTGTGTGCCACATGGCAGAAGAGCAGGGAAGAGACAACAAAGCATCGCCATCACAATGCCACGGTCAATTCCCTCATCATTTTTGCTTTGAAACTCCAAAAGATAAACAGAACAACGCAACCCTTCCACGGCTTCCCTTAATCTCAACCTTCATCCTGTATATGTATGTATTGTTGGGCCATTATCATGTTTATCAACAGCGAATCTCTATAATTTCTTGAGTTATGTAACCTTGTCCTGTGTGGACACTACCTTGTCCCAGTCCTCAAACTTGGACAACTTTGTTTTACGTGCCACTTGATTATTCGATAGGCTGATTAATTTATTCTCAAGGGATACTAATTAATCACAGTAGCTAGTTTGCTTTTATA

Coding sequence (CDS)

ATGGGTGACCATGATAATGTTTTTGATGAAAGCTACGAACAGACTAATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTGGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCGCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCAGAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGTGTTGGTACTGTTGTAGGACATGGAGGAATGCATAATCGCTTCGATGGAATACTATTACCATGGAATGAGTATGCTGTTACCTCGTGTACTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAGTATTGGGATTCTTGTAATTATACTGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCTGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAAAAGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATTGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGACCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTTTATGATTGGGGTGACTCAGTGCATGACGAGATAGTAAAATTAATAGGCATTCGAAAATGTCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAGATCGGAGATGGTTCTTGGTGTCCTGGTATGGAATGGGCTCTTGCAACCTCTGGCCGCAGATATGCAGTGTGGCAGAAATAA

Protein sequence

MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK
Homology
BLAST of PI0021102 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 2.5e-207
Identity = 320/413 (77.48%), Postives = 373/413 (90.31%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MG ++NVFDE  +QT+ G VIR+GRE++ QA+NWESHK DWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL+KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSCTGGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNY GH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVK Q WRL D QGKPPGV+GWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDIN 360
           TGSTQ HWPFP++H+MEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLI IR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCP-GMEWALATSGRRYAVWQK 413
           SRS+V++L+A S+LY+AI+GEK+CMK+GDGSWCP G +W LATSG RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of PI0021102 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.5e-119
Identity = 199/397 (50.13%), Postives = 270/397 (68.01%), Query Frame = 0

Query: 23  NGREILFQAFNWESHKCD-WWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q FNWES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAV 142
           LNS YG+ D LK  ++K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCT---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A+KP F++GEYWDS +YT  ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAVKR-QLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFPANHIME 322
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH +      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGEKLCMKIGDGSWCP---GMEWALATSGRRYAVWQ 412
           II EK+ MKIG G + P      W++A  GR Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of PI0021102 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.0e-104
Identity = 185/401 (46.13%), Postives = 261/401 (65.09%), Query Frame = 0

Query: 27  ILFQAFNWE-SHKCDWWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSS 86
           +LFQ FNWE S K  W+ +L+  + D+A +G T  WLPPP+ S++P+GYLP  LY L++S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 87  -YGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNEYA 146
            YGS++ LK+L+    +  ++ +ADIVINHR        G++  F+G        W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 147 V----TSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
           +    T+ + G GN  +G+ +   P+IDH  P V+R++  W+ WL++ +GF  +RFDF +
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 207 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHD--LDYNQDGHRQRIINWIDGTGQLSAA 266
           G++    K Y+E  KP F++GE WDS +Y G D   +YNQD HR  ++NW++  G    A
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVNWVESAGGAITA 264

Query: 267 FDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFPANH 326
           FDFTTKG+LQ AV+ +LWRL D  GKPPG+IG  P  AVTF+DNHDTGSTQ  WPFP++ 
Sbjct: 265 FDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 327 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEARSDL 386
           +M+GYAYILTHPG P++FYDHF+DWG  + ++I KL  IR    IN +S+VKI+ +  DL
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNGINEKSTVKIMASEGDL 384

Query: 387 YSAIIGEKLCMKIGD----GSWCPGMEWALATSGRRYAVWQ 412
           Y A I  K+ +KIG     G+  P     +ATSG+ YAVW+
Sbjct: 385 YVAKIDNKIMVKIGPKMDLGNLIPS-NLHVATSGQDYAVWE 421

BLAST of PI0021102 vs. ExPASy Swiss-Prot
Match: P27932 (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 380.2 bits (975), Expect = 3.0e-104
Identity = 183/403 (45.41%), Postives = 257/403 (63.77%), Query Frame = 0

Query: 26  EILFQAFNWESHKCD--WWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           +ILFQ FNW+S K    W+  L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 86  SS-YGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNE 145
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 146 YAV----TSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  + G G+R TG +F   P+IDH  P V+R++  WL+WLR  VGF  +R DF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208

Query: 206 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLD-YNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++ A+P F + E W+S +Y G      NQDG RQ ++NW+   G  + 
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268

Query: 266 AFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFPAN 325
           AFDFTTKG+LQ AV+ +LWR+RD  GK PG+IGW+P +AVTF+DNHDTGSTQ  WPFP++
Sbjct: 269 AFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSD 328

Query: 326 HIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEARSD 385
            ++ GYAYILTHPG+P +FYDH +DW  ++  EI  L   RK   IN+ S +++L A SD
Sbjct: 329 KVILGYAYILTHPGVPCIFYDHVFDW--NLKQEINALAATRKRNGINAGSKLRVLAAESD 388

Query: 386 LYSAIIGEKLCMKIGD----GSWCPGMEWALATSGRRYAVWQK 413
           +Y A++ E++  KIG     G+  P  ++ +   G  Y VW+K
Sbjct: 389 MYVAMVDERVITKIGPRIDVGNIIPS-DFHIVAHGNDYCVWEK 428

BLAST of PI0021102 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 378.6 bits (971), Expect = 8.8e-104
Identity = 187/405 (46.17%), Postives = 260/405 (64.20%), Query Frame = 0

Query: 26  EILFQAFNWES--HKCDWWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           ++LFQ FNWES  H   W+  L  KV DIA +G T  WLPP + S+A QGY+P  LY L+
Sbjct: 25  QVLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGITHVWLPPASQSVAEQGYMPGRLYDLD 84

Query: 86  SS-YGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNE 145
           +S YG++  LK+L+  +    V+AIADIVINHR        G++  F+G      L W  
Sbjct: 85  ASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGP 144

Query: 146 YAV----TSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
           + +         G GN  TG +F   P+IDH    V+++++ WL WL++ +GF  +RFDF
Sbjct: 145 HMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLNWLKADIGFDGWRFDF 204

Query: 206 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDL-DYNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   K YI+ ++P F++ E W S  Y G    + NQD HRQ ++NW+D  G    
Sbjct: 205 AKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGGKGP 264

Query: 266 A--FDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFP 325
           A  FDFTTKG+L  AV+ +LWRLR   GK PG+IGWWP++AVTF+DNHDTGSTQ  WPFP
Sbjct: 265 ATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHMWPFP 324

Query: 326 ANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEAR 385
           ++ +M+GYAYILTHPG P +FYDHF+DWG  + +EI +L+ +R    I++ S ++I+EA 
Sbjct: 325 SDRVMQGYAYILTHPGTPCIFYDHFFDWG--LKEEIDRLVSVRTRHGIHNESKLQIIEAD 384

Query: 386 SDLYSAIIGEKLCMKIGD----GSWCPGMEWALATSGRRYAVWQK 413
           +DLY A I  K+ +K+G     G+  PG  + +A  G  YAVW+K
Sbjct: 385 ADLYLAEIDGKVIVKLGPRYDVGNLIPG-GFKVAAHGNDYAVWEK 426

BLAST of PI0021102 vs. ExPASy TrEMBL
Match: A0A5A7VG02 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 6.5e-243
Identity = 391/412 (94.90%), Postives = 399/412 (96.84%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQDGHRQR
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 295

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFLDNHD
Sbjct: 296 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 355

Query: 301 TGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDIN 360
           TGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK QDIN
Sbjct: 356 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 415

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 416 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 467

BLAST of PI0021102 vs. ExPASy TrEMBL
Match: A0A1S3BW59 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 6.5e-243
Identity = 391/412 (94.90%), Postives = 399/412 (96.84%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDIN 360
           TGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK QDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 437

BLAST of PI0021102 vs. ExPASy TrEMBL
Match: A0A5D3D8R2 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.6e-241
Identity = 391/416 (93.99%), Postives = 399/416 (95.91%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQ    DG
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 295

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFL
Sbjct: 296 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 355

Query: 301 DNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKC 360
           DNHDTGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK 
Sbjct: 356 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 415

Query: 361 QDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 416 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 471

BLAST of PI0021102 vs. ExPASy TrEMBL
Match: E5GCD3 (Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.6e-241
Identity = 391/416 (93.99%), Postives = 399/416 (95.91%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKC 360
           DNHDTGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK 
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 416

BLAST of PI0021102 vs. ExPASy TrEMBL
Match: A0A1S4DZE2 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.0e-240
Identity = 391/420 (93.10%), Postives = 399/420 (95.00%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQ------ 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQ      
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQVMITAL 240

Query: 241 --DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRA 300
             DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRA
Sbjct: 241 NADGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA 300

Query: 301 VTFLDNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIG 360
           VTFLDNHDTGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI 
Sbjct: 301 VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID 360

Query: 361 IRKCQDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           IRK QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 361 IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 420

BLAST of PI0021102 vs. NCBI nr
Match: KAA0064621.1 (putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 849.4 bits (2193), Expect = 1.3e-242
Identity = 391/412 (94.90%), Postives = 399/412 (96.84%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQDGHRQR
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 295

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFLDNHD
Sbjct: 296 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 355

Query: 301 TGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDIN 360
           TGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK QDIN
Sbjct: 356 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 415

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 416 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 467

BLAST of PI0021102 vs. NCBI nr
Match: XP_008452915.1 (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 849.4 bits (2193), Expect = 1.3e-242
Identity = 391/412 (94.90%), Postives = 399/412 (96.84%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDIN 360
           TGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK QDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 437

BLAST of PI0021102 vs. NCBI nr
Match: ADN34132.1 (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 843.6 bits (2178), Expect = 7.3e-241
Identity = 391/416 (93.99%), Postives = 399/416 (95.91%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKC 360
           DNHDTGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK 
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 416

BLAST of PI0021102 vs. NCBI nr
Match: TYK19971.1 (putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 843.6 bits (2178), Expect = 7.3e-241
Identity = 391/416 (93.99%), Postives = 399/416 (95.91%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQ    DG
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 295

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRAVTFL
Sbjct: 296 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 355

Query: 301 DNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKC 360
           DNHDTGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI IRK 
Sbjct: 356 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 415

Query: 361 QDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 416 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 471

BLAST of PI0021102 vs. NCBI nr
Match: XP_016901343.1 (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 842.0 bits (2174), Expect = 2.1e-240
Identity = 391/420 (93.10%), Postives = 399/420 (95.00%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YEQT+TGAVIRNGREILFQAFNWESHKCDW GNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQ------ 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNY GHDLDYNQ      
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQVMITAL 265

Query: 241 --DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRA 300
             DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVK QLWRL DC+GKPPGV+GWWPSRA
Sbjct: 266 NADGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA 325

Query: 301 VTFLDNHDTGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIG 360
           VTFLDNHDTGSTQ HWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLI 
Sbjct: 326 VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID 385

Query: 361 IRKCQDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPGMEWALATSGRRYAVWQK 413
           IRK QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCPG EW LAT G RYAVWQK
Sbjct: 386 IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 445

BLAST of PI0021102 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 722.6 bits (1864), Expect = 1.8e-208
Identity = 320/413 (77.48%), Postives = 373/413 (90.31%), Query Frame = 0

Query: 1   MGDHDNVFDESYEQTNTGAVIRNGREILFQAFNWESHKCDWWGNLECKVHDIAKSGFTSA 60
           MG ++NVFDE  +QT+ G VIR+GRE++ QA+NWESHK DWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL+KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCTGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSCTGGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNY GH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVK Q WRL D QGKPPGV+GWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQTHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDIN 360
           TGSTQ HWPFP++H+MEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLI IR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCP-GMEWALATSGRRYAVWQK 413
           SRS+V++L+A S+LY+AI+GEK+CMK+GDGSWCP G +W LATSG RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of PI0021102 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 430.3 bits (1105), Expect = 1.8e-120
Identity = 199/397 (50.13%), Postives = 270/397 (68.01%), Query Frame = 0

Query: 23  NGREILFQAFNWESHKCD-WWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q FNWES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAV 142
           LNS YG+ D LK  ++K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCT---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAAKPMFSIGEYWDSCNYTGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A+KP F++GEYWDS +YT  ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAVKR-QLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFPANHIME 322
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH +      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGEKLCMKIGDGSWCP---GMEWALATSGRRYAVWQ 412
           II EK+ MKIG G + P      W++A  GR Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of PI0021102 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 359.0 bits (920), Expect = 5.1e-99
Identity = 186/404 (46.04%), Postives = 255/404 (63.12%), Query Frame = 0

Query: 27  ILFQAFNWESHKCD--WWGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 86
           +LFQ+FNWES K +  ++ +L   + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 87  S-YGSEDMLKALLQKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNEY 146
           S YGSE  LK+L++ + Q  ++A+ADIVINHR          +  F+G      L W+  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 147 AVTSCTG-----GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 206
            V  C       G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+
Sbjct: 147 FV--CRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDY 206

Query: 207 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYTGH-DLDYNQDGHRQRIINWI-DGTGQLS 266
            RG+++   K Y++   P F++GE WD   Y G   LDY+Q+ HR  +  WI +  G + 
Sbjct: 207 VRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVL 266

Query: 267 AAFDFTTKGVLQEAVKRQLWRLRDCQGKPPGVIGWWPSRAVTFLDNHDTGSTQTHWPFPA 326
            AFDFTTKG+LQ AVK +LWRL+D QGKPPG+IG  P  AVTF+DNHDT  T   W FP+
Sbjct: 267 TAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPS 326

Query: 327 NHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIGIRKCQDINSRSSVKILEARS 386
           + ++ GY YILTHPG P +FY+H+ +WG  + + I KL+ IR    I S SSV I  A +
Sbjct: 327 DKVLLGYVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEA 386

Query: 387 DLYSAIIGEKLCMKIGD----GSWCPGMEWALATSGRRYAVWQK 413
           DLY A+I +K+ MKIG     G+  P   +ALA SG  +AVW+K
Sbjct: 387 DLYLAMIDDKVIMKIGPKQDVGTLVPS-NFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LFG12.5e-20777.48Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
Q94A412.5e-11950.13Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
P178591.0e-10446.13Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1[more]
P279323.0e-10445.41Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2... [more]
P040638.8e-10446.17Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7VG026.5e-24394.90Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440... [more]
A0A1S3BW596.5e-24394.90Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1[more]
A0A5D3D8R23.6e-24193.99Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 ... [more]
E5GCD33.6e-24193.99Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S4DZE21.0e-24093.10Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0064621.11.3e-24294.90putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa][more]
XP_008452915.11.3e-24294.90PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo][more]
ADN34132.17.3e-24193.99cytosolic alpha-amylase [Cucumis melo subsp. melo][more]
TYK19971.17.3e-24193.99putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa][more]
XP_016901343.12.1e-24093.10PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G76130.11.8e-20877.48alpha-amylase-like 2 [more]
AT1G69830.11.8e-12050.13alpha-amylase-like 3 [more]
AT4G25000.15.1e-9946.04alpha-amylase-like [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006046Alpha amylasePRINTSPR00110ALPHAAMYLASEcoord: 187..198
score: 35.54
coord: 290..302
score: 53.54
coord: 103..114
score: 52.04
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 355..412
e-value: 4.7E-27
score: 105.9
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 357..411
e-value: 3.1E-20
score: 72.2
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 26..354
e-value: 2.7E-23
score: 93.4
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 52..114
e-value: 5.7E-9
score: 36.0
IPR013775Alpha-amylase, plantPIRSFPIRSF001028Alpha-amylase_plantcoord: 6..412
e-value: 2.1E-181
score: 601.4
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 359..412
e-value: 1.0E-18
score: 69.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 26..358
e-value: 3.1E-117
score: 393.9
NoneNo IPR availablePANTHERPTHR43447:SF18ALPHA-AMYLASE 2-RELATEDcoord: 22..412
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 22..412
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 27..365
e-value: 1.45219E-165
score: 464.772
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 359..412
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 33..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021102.2PI0021102.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0043169 cation binding