PI0021042 (gene) Melon (PI 482460) v1

Overview
NamePI0021042
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionDol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Locationchr08: 16097884 .. 16101485 (-)
RNA-Seq ExpressionPI0021042
SyntenyPI0021042
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGAATTGCCGTAGCCGCCATCGTCACTTTGTGGGTGGTTCCGATCTCCATCCTCGTCAACCGCATTCTTCCCAATTCTTATATGGTAAGTATTTTCGTCAATTTCATTTCGAATTTTTAAAATTTGGACTTTGTTCGTTTTTGCAATTTCGTACTAAAATCAATTGAAAAACTTACAATATTGATCTCGATTGATGTTTAAGGACGAGATATTTCATGTGCCTCAAGCTCAGAAATACTGCAATGGTAACTTCAGAAGTTGGGATCCCATGATTACTACTCCTCCTGGATTGTACGTTCGTAATTCTATTCTGGATTATGAGGCTATCGGTCTATTCATTTATTTGTCAGGAAAAATGCCGTAGCTGCCCCCAATATCCAATATGGAACAGTGAAATATTTGATTGTTTTCTTAATTTTGAATAACTCAATTGTCTCTGCGAGAAACTAACAATTAACAAATAAGAAATATTGGTCTACTATCAAGGTAGCATCACTTGGGTCTTTTGACAGAACTTTGTGGTAATGATAGTATGTGTTTTCACAGGATTGTTAATACAAGGAATCTCAAAATTTCTACAGGTACTATCTTTCGCTTGCACATGTTGCTTCTTTGTTTCCCGGCATTGTCTTTACTCGAGCAGCACCCTCGTTTTCTGCTGCCTGTTCCATTCAGATTCTTCGTTCTATCAATGGGATTTTGGCAGTTTTGTGCAGTGTACTTGTATATGAGATAATTTCCCAATTAAGGCCAAATCTTGATGATAAAAGGGCAACGACTTATGCTGTGATTTTGGCACTTTATCCACTCCATTGGTTCTTCACTTTTCTGTATTACACTGATGTTGCATCACTTACAGCCGTCCTTGCTATGTACTTAGCATGCTTGAAGAAGAATTACTGGTTGAGTGCATTGGTAAGGAGCTTTCTTATACTTTTCCATGATAAGTTCTTAAGTTTGTTGCACATTCATTATTCCTTATCTTGGGGTATGAAATTGATTCAATGCGAACAAAGTCAGAGGAAACAAATGTTATATGGATGTATGCACATAGATATCCTGTATGTATAGTTGGATGAAATAATTCCTCTCATCAATTTATGAGTCCTTGGGTGGTTTCTAGAATTATGGTGATGCAGAGTTTCTTGAGTTGATAAGGACACTTGTTTTGCAGTCAACAGTAGTCAAGAGAGCACCTGTAGTAGTAGGAGTAGAGAAGAGTTTATTGTGATTTGTTGTAACCCATCAAATGGAACTTTGCTATTTTTATGTTGGAATTTCTCCATTCGATTCTGCAATCTTTTACTCTTCTGATAACTTAGTTTCATACAGTTTGGTATCTTTGCAGTTGTCATTCGACAAACAAATGTTATATGGATGCTTTTTGTAGCATGCTCTGGGGTTATTGATACTACTCTGACTTTCTACGATGATAGTATACAAATGCATGAGAATGATGTAATGGTGGAGGAAAGTAGTTCACTGATTGCTAAAGGCAGTTTAACTTCTAAAGCAAACTTGCGAAAGAGAAAGTTTAGTCCTATAAAGGATGTGGGTAAACAAGTTCCACAGAGCACAAGATTCTCATCAATAAACCAGAAACTAGGTATTATTTCACAGTTTTCAATCTGTTTCCATTAAAAATTTAAATTTTAAGCTGTTTTATGTAGTTCATTTGCAGAAATGGTTGTCTGAAGTTTTGTTGCCACAAACGTATATCTTAAGCAGTAACTCGCCCCTTTTCTTCTGTTCCTTTGCTGCTCCGAGTTTCTGTATTTTTATTGATCATTCCTGCACTTCTGTTGGCAATTCTGTTACAGGTTTAATGGATGAGATTAGGGCCATGATTTTGACCATGTGGCGTATGAAATTTAAGCTCTTAGTTTCATTTTTCCCCTTTGTGATTGTGCTTATGGCCTTTTTCGCTTTTGTTCGTTGGAATGGAAGTATAGTTCTTGGTAATTATCATCTGACACCCTCAATTTTGTTTGCATCAAGTGCCCTTTATCATTTATTATTTTAAATAGTTTATTTATTGATTTTTTACTATAATTTTTATCAGAGGAAAAAAACTTATTGTTATTACTATCTCAAATTACAGGTGCGAAGGAAGCACATGCAGTCTCTCTGCATTTTGCACAGATTATGTATTTGGTCTTTTTGCTGCTCTTCTTATGGCTCCAATGCACTGTAATCTAAGTCAAGTCATAGATTTATTTCACTCATTCTGGAAAGGCAGACCTCTTAGCTTTTTTCAAGTTTTCATTGCTCTTCTTGCCGGCTTCATCTCTGTCCAATATTTCAGGTCAGTTCGGGTTCAGAGAAACATAGTATTGGAGGGATTATATTTCATTTCTCATACTGCATGATACATCTCTGAGATCATTCAGCTAAAGAATATCAATAATTAAATAAAAGGAATACTGGTTTTGAAGGGGCGGCTTTGTTGGCTAAGCCCTGTGGTCTCCAAGTAACACATACCCAGTGGTACCGTGTTTAGAGCTTTGGATAAGCTTAATAAGAAAACCCTTGTTGTCTTCGAAGCCCGGAGCCTTGAGGCAGCGTGGACTTTATAGAAAGTGAAAAGGAGTACTGCTTTTTGTTTTTGTTAAGGGATTGACCAACGATAAGTTATCACTTGTCAGGCATGCAATGTCTTGATTGTTCTTCAAGATTCCATTTAAATTCTTCCAGTGCCTTTTGTCTCCTTTTCTTTTCCTTGAAAATAAACGCTGAGATTTTGTGACTAGAGTTGGTGGTGAAATTCTTCTAAACAAATTGCCAATTGCATGCTTCATTTTCCTTTTCATTCATCACATATTATTTGGTCTGTTGCAGCATAGCTCACCCCTATCTTCTAGCTGACAATCGGCATTACCCCTTCTACCTCTGGCGTAAAGTTATCAACGCACACTGGTCATCAAAGTACCTTCTCGTTCCAGCATATATTTGTTCGTGGTTTTCCATCATCAAGATACTAGGTGAGTGGCTTATCTAGTCTTCTACTAATGCCATGAAAATCGGAGATTTATGGATGGGATCTTATCTACTGTTTTCGTTTGGTGTAAATTTCAGCATATTTTTATTCATTATAGCCCAGTTGATATAAATTTCCAATTGGAGAGTTCTTTTGTAATGCAGCAATAGGTGTTGGAGTTCACTCTCCTTTATTTCATGTATCATTGAGATTGTTTCTTATTAAAAAAAAAGACACTGATGCCAAGAAGATATTGTAAACTTCACTCATATAAAAAGAAAGAACCGAATAACACAACTGTATTGTATTTGCTCTATTTGTACCAGCTTCTTTCCGCTGACTAAATTTTGCTCTGGCAGGGAAATCCCAGAGAAAAATCTGGGTAGTGGCTTATTTCTTAGCGACAGCTGCGGTTCTTGTTCCTGCTCCTTTGATCGAGTTTAGATACTTCACAACTCCATTTTACTTCTTGATGCTTCATTCTCAAATGAATGGTAGACTAAATTGGCCGTTGGTTGCACTTCTTTACATAGCTTTGAATGCCTTCACAATGTTCATGTTCTTGTTTCGTCCATTTTACTGGGAAAATGAAACTGGAAAGCAGAGGTTCATATGGTAG

mRNA sequence

ATGGGTAGAATTGCCGTAGCCGCCATCGTCACTTTGTGGGTGGTTCCGATCTCCATCCTCGTCAACCGCATTCTTCCCAATTCTTATATGGACGAGATATTTCATGTGCCTCAAGCTCAGAAATACTGCAATGGTAACTTCAGAAGTTGGGATCCCATGATTACTACTCCTCCTGGATTGTACTATCTTTCGCTTGCACATGTTGCTTCTTTGTTTCCCGGCATTGTCTTTACTCGAGCAGCACCCTCGTTTTCTGCTGCCTGTTCCATTCAGATTCTTCGTTCTATCAATGGGATTTTGGCAGTTTTGTGCAGTGTACTTGTATATGAGATAATTTCCCAATTAAGGCCAAATCTTGATGATAAAAGGGCAACGACTTATGCTGTGATTTTGGCACTTTATCCACTCCATTGGTTCTTCACTTTTCTGTATTACACTGATGTTGCATCACTTACAGCCGTCCTTGCTATGTACTTAGCATGCTTGAAGAAGAATTACTGGTTGAGTGCATTGTTTGGTATCTTTGCAGTTGTCATTCGACAAACAAATGTTATATGGATGCTTTTTGTAGCATGCTCTGGGGTTATTGATACTACTCTGACTTTCTACGATGATAGTATACAAATGCATGAGAATGATGTAATGGTGGAGGAAAGTAGTTCACTGATTGCTAAAGGCAGTTTAACTTCTAAAGCAAACTTGCGAAAGAGAAAGTTTAGTCCTATAAAGGATGTGGGTAAACAAGTTCCACAGAGCACAAGATTCTCATCAATAAACCAGAAACTAGGTTTAATGGATGAGATTAGGGCCATGATTTTGACCATGTGGCGTATGAAATTTAAGCTCTTAGTTTCATTTTTCCCCTTTGTGATTGTGCTTATGGCCTTTTTCGCTTTTGTTCGTTGGAATGGAAGTATAGTTCTTGGAAGCACATGCAGTCTCTCTGCATTTTGCACAGATTATGTATTTGGTCTTTTTGCTGCTCTTCTTATGGCTCCAATGCACTGTAATCTAAGTCAAGTCATAGATTTATTTCACTCATTCTGGAAAGGCAGACCTCTTAGCTTTTTTCAAGTTTTCATTGCTCTTCTTGCCGGCTTCATCTCTGTCCAATATTTCAGCATAGCTCACCCCTATCTTCTAGCTGACAATCGGCATTACCCCTTCTACCTCTGGCGTAAAGTTATCAACGCACACTGGTCATCAAAGTACCTTCTCGTTCCAGCATATATTTGTTCGTGGTTTTCCATCATCAAGATACTAGGGAAATCCCAGAGAAAAATCTGGGTAGTGGCTTATTTCTTAGCGACAGCTGCGGTTCTTGTTCCTGCTCCTTTGATCGAGTTTAGATACTTCACAACTCCATTTTACTTCTTGATGCTTCATTCTCAAATGAATGGTAGACTAAATTGGCCGTTGGTTGCACTTCTTTACATAGCTTTGAATGCCTTCACAATGTTCATGTTCTTGTTTCGTCCATTTTACTGGGAAAATGAAACTGGAAAGCAGAGGTTCATATGGTAG

Coding sequence (CDS)

ATGGGTAGAATTGCCGTAGCCGCCATCGTCACTTTGTGGGTGGTTCCGATCTCCATCCTCGTCAACCGCATTCTTCCCAATTCTTATATGGACGAGATATTTCATGTGCCTCAAGCTCAGAAATACTGCAATGGTAACTTCAGAAGTTGGGATCCCATGATTACTACTCCTCCTGGATTGTACTATCTTTCGCTTGCACATGTTGCTTCTTTGTTTCCCGGCATTGTCTTTACTCGAGCAGCACCCTCGTTTTCTGCTGCCTGTTCCATTCAGATTCTTCGTTCTATCAATGGGATTTTGGCAGTTTTGTGCAGTGTACTTGTATATGAGATAATTTCCCAATTAAGGCCAAATCTTGATGATAAAAGGGCAACGACTTATGCTGTGATTTTGGCACTTTATCCACTCCATTGGTTCTTCACTTTTCTGTATTACACTGATGTTGCATCACTTACAGCCGTCCTTGCTATGTACTTAGCATGCTTGAAGAAGAATTACTGGTTGAGTGCATTGTTTGGTATCTTTGCAGTTGTCATTCGACAAACAAATGTTATATGGATGCTTTTTGTAGCATGCTCTGGGGTTATTGATACTACTCTGACTTTCTACGATGATAGTATACAAATGCATGAGAATGATGTAATGGTGGAGGAAAGTAGTTCACTGATTGCTAAAGGCAGTTTAACTTCTAAAGCAAACTTGCGAAAGAGAAAGTTTAGTCCTATAAAGGATGTGGGTAAACAAGTTCCACAGAGCACAAGATTCTCATCAATAAACCAGAAACTAGGTTTAATGGATGAGATTAGGGCCATGATTTTGACCATGTGGCGTATGAAATTTAAGCTCTTAGTTTCATTTTTCCCCTTTGTGATTGTGCTTATGGCCTTTTTCGCTTTTGTTCGTTGGAATGGAAGTATAGTTCTTGGAAGCACATGCAGTCTCTCTGCATTTTGCACAGATTATGTATTTGGTCTTTTTGCTGCTCTTCTTATGGCTCCAATGCACTGTAATCTAAGTCAAGTCATAGATTTATTTCACTCATTCTGGAAAGGCAGACCTCTTAGCTTTTTTCAAGTTTTCATTGCTCTTCTTGCCGGCTTCATCTCTGTCCAATATTTCAGCATAGCTCACCCCTATCTTCTAGCTGACAATCGGCATTACCCCTTCTACCTCTGGCGTAAAGTTATCAACGCACACTGGTCATCAAAGTACCTTCTCGTTCCAGCATATATTTGTTCGTGGTTTTCCATCATCAAGATACTAGGGAAATCCCAGAGAAAAATCTGGGTAGTGGCTTATTTCTTAGCGACAGCTGCGGTTCTTGTTCCTGCTCCTTTGATCGAGTTTAGATACTTCACAACTCCATTTTACTTCTTGATGCTTCATTCTCAAATGAATGGTAGACTAAATTGGCCGTTGGTTGCACTTCTTTACATAGCTTTGAATGCCTTCACAATGTTCATGTTCTTGTTTCGTCCATTTTACTGGGAAAATGAAACTGGAAAGCAGAGGTTCATATGGTAG

Protein sequence

MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGLYYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLDDKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIRQTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFSPIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFVRWNGSIVLGSTCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFFQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSIIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALLYIALNAFTMFMFLFRPFYWENETGKQRFIW
Homology
BLAST of PI0021042 vs. ExPASy Swiss-Prot
Match: Q8L638 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=ALG10 PE=1 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.4e-159
Identity = 294/514 (57.20%), Postives = 367/514 (71.40%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MG++AVAAI +LWV+P+SI+VN I+P  YMDEIFHVPQAQ+YCNGNFRSWDPMITTPPGL
Sbjct: 1   MGKLAVAAITSLWVIPMSIIVNHIVPEPYMDEIFHVPQAQQYCNGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPG-IVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNL 120
           YYLSLAHVASLFPG ++    + SFS ACS  +LRS N + AVLC VLVYEII  L PNL
Sbjct: 61  YYLSLAHVASLFPGMLLMENTSQSFSEACSTSVLRSTNAVSAVLCGVLVYEIIRFLGPNL 120

Query: 121 DDKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVI 180
            D++AT  A++++LYPLHWFFTFLYYTDVASLTAVLAMYL CLK+ Y LSALFG  AV I
Sbjct: 121 SDRKATFMALVMSLYPLHWFFTFLYYTDVASLTAVLAMYLTCLKRRYVLSALFGTLAVFI 180

Query: 181 RQTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKF 240
           RQTNV+WMLFVACSG++D TL   D S Q  + +V  E   S   KG+ T ++NLRKRK 
Sbjct: 181 RQTNVVWMLFVACSGILDFTL---DSSKQKGKQEVNQELHQSSNKKGA-TLRSNLRKRKS 240

Query: 241 SPIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAF 300
               D            S      L+ +I  +I T W +K+++L+ F PF+ V++AF  F
Sbjct: 241 DISSDTSDPFNHGQTVPSTEDTSDLVYDIYTVISTSWNLKWRILIKFSPFIFVVVAFGIF 300

Query: 301 VRWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLS 360
           + WNG IVLG+      SL  F     F L +AL  AP+H +++Q+   FH   +   LS
Sbjct: 301 ILWNGGIVLGAKEAHVVSLH-FAQIMYFSLVSALFTAPLHFSVNQLRHQFHQLHRNWSLS 360

Query: 361 FFQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWF 420
                +AL+AGF+SV +FS+AHPYLLADNRHYPFYLWRK+INAHW  KY+LVP Y+ SWF
Sbjct: 361 LILTLVALVAGFVSVHFFSLAHPYLLADNRHYPFYLWRKIINAHWLMKYILVPVYVYSWF 420

Query: 421 SIIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHS--QMNGRLNWPL 480
           SI+ +L K++R+ W++ YFLAT  VLVP PLIEFRY+T PFY  MLHS  + +    W L
Sbjct: 421 SILTLLAKTRRQTWILVYFLATCGVLVPTPLIEFRYYTIPFYLFMLHSCVRSSSFATWLL 480

Query: 481 VALLYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           +  +++++N FTM MFLFRPF W +E G QRFIW
Sbjct: 481 IGTIFVSINVFTMAMFLFRPFKWSHEDGVQRFIW 509

BLAST of PI0021042 vs. ExPASy Swiss-Prot
Match: Q3UGP8 (Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg10b PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 7.1e-55
Identity = 177/506 (34.98%), Postives = 246/506 (48.62%), Query Frame = 0

Query: 22  NRILPNSYMDEIFHVPQAQKYCNGNF--RSWDPMITTPPGLYYLSLAHV--ASLFPGIVF 81
           +R L   YMDEIFH+PQAQ+YC G F    WDPMITT PGLY +S+  V  AS   G   
Sbjct: 28  SRALREPYMDEIFHLPQAQRYCEGRFSLSQWDPMITTLPGLYLVSVGVVKPASWLLGW-- 87

Query: 82  TRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLDDKRATTY-----AVILA 141
                S    CSI +LR +N + +V    L+Y +  +++P   +K +++       + LA
Sbjct: 88  -----SEHVICSIGVLRFVNLLFSVGNFYLLYLLFRKVQPR--NKASSSIQRILSTLTLA 147

Query: 142 LYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIRQTNVIWMLFVAC 201
           ++P  +FF FLYYT+  S+   L  YL CL  N+  SAL G    + RQTN+IW  F  C
Sbjct: 148 VFPTLYFFNFLYYTEAGSVFFTLFAYLMCLYGNHRTSALLGFCGFMFRQTNIIWAAF--C 207

Query: 202 SGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFSPIKDVGKQVPQS 261
           +G +                         +  K S   K  L+K+K        +  P  
Sbjct: 208 AGHL-------------------------IAQKCSEAWKIELQKKKEE------RLAPTK 267

Query: 262 TRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFVRWNGSIVLGSTC 321
              S + + L  +  + AM L   RM F L    +P+V++L+AFFAFV  NG IV+G   
Sbjct: 268 GPLSELRRVLQFL-LVYAMSLKNLRMLFLLT---WPYVLLLLAFFAFVVVNGGIVVGDRS 327

Query: 322 SLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFFQVFIALLAGFIS 381
           S  A   F   + F  F A    P   +L++V       WK R     Q  +  L   + 
Sbjct: 328 SHEACLHFPQLFYFFSFTAFFSFPHLLSLTKVKTFLSLVWKRR----VQFSVVTLVSILL 387

Query: 382 VQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSIIKILGKSQRKIW 441
           V  F+  H YLLADNRHY FY+W++V   H   KYLLVPAYI + ++I   L K++   W
Sbjct: 388 VWKFTYVHKYLLADNRHYTFYVWKRVFQRHEVVKYLLVPAYIFAGWAIADSL-KAKSIFW 447

Query: 442 VVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL--------LYIAL 501
            + +F+   A  VP  L+EFRYF  P+           RLN PL  +         Y  +
Sbjct: 448 NLMFFVCLVASTVPQKLLEFRYFILPYIIY--------RLNIPLPPISRLVCELGCYTVV 474

Query: 502 NAFTMFMFLFRPFYWENETGKQRFIW 508
           N  T ++FL + F W N    QRF+W
Sbjct: 508 NFVTFYIFLNKTFQWPNSQDIQRFMW 474

BLAST of PI0021042 vs. ExPASy Swiss-Prot
Match: Q5BKT4 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG10 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 5.1e-53
Identity = 167/503 (33.20%), Postives = 239/503 (47.51%), Query Frame = 0

Query: 22  NRILPNSYMDEIFHVPQAQKYCNGNF--RSWDPMITTPPGLYYLSLAHVASLFPGI-VFT 81
           +R L   YMDEIFH+PQAQ+YC G+F    WDPMITT PGLY +S+  +    P I +F 
Sbjct: 28  SRALREPYMDEIFHLPQAQRYCEGHFSLSQWDPMITTLPGLYLVSIGVIK---PAIWIF- 87

Query: 82  RAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLDDKRATTY-----AVILAL 141
               S    CSI +LR +N + +V    L+Y +  +++P   +K A++       + LA+
Sbjct: 88  --GWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFCKVQPR--NKAASSIQRVLSTLTLAV 147

Query: 142 YPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIRQTNVIWMLFVACS 201
           +P  +FF FLYYT+  S+   L  YL CL  N+  SA  G    + RQTN+IW +F A +
Sbjct: 148 FPTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGFCGFMFRQTNIIWAVFCAGN 207

Query: 202 GVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFSPIKDVGKQVPQST 261
            +       +   +Q  E+                         +  PIK          
Sbjct: 208 VIAQKLTEAWKTELQKKED-------------------------RLPPIK---------- 267

Query: 262 RFSSINQKLGLMDEIRAMI--LTMWRMKFK----LLVSFFPFVIVLMAFFAFVRWNGSIV 321
                    G   E R ++  L  + M FK    LL+  +P++++   F AFV  NG IV
Sbjct: 268 ---------GPFAEFRKILQFLLAYSMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIV 327

Query: 322 LGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFFQVFIALL 381
           +G   S  A   F   + F  F      P   + S++       WK R L     F+  L
Sbjct: 328 IGDRSSHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKRRIL----FFVVTL 387

Query: 382 AGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSIIKILGKS 441
                V  F+ AH YLLADNRHY FY+W++V   + + KYLLVPAYI + +SI   L KS
Sbjct: 388 VSVFLVWKFTYAHKYLLADNRHYTFYVWKRVFQRYETVKYLLVPAYIFAGWSIADSL-KS 447

Query: 442 QRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALLYIALNAF 501
           +   W + +F+    V+VP  L+EFRYF  P+    L+  +            Y  +N  
Sbjct: 448 KSIFWNLMFFICLFTVIVPQKLLEFRYFILPYVIYRLNIPLPPTSRLICELSCYAVVNFI 473

Query: 502 TMFMFLFRPFYWENETGKQRFIW 508
           T F+FL + F W N    QRF+W
Sbjct: 508 TFFIFLNKTFQWPNSQDIQRFMW 473

BLAST of PI0021042 vs. ExPASy Swiss-Prot
Match: O88788 (Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Rattus norvegicus OX=10116 GN=Alg10b PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.5e-52
Identity = 173/512 (33.79%), Postives = 246/512 (48.05%), Query Frame = 0

Query: 22  NRILPNSYMDEIFHVPQAQKYCNGNF--RSWDPMITTPPGLYYLSLAHV--ASLFPGIVF 81
           +R L   YMDEIFH+PQAQ+YC G F    WDPMITT PGLY +S+  V  AS   G   
Sbjct: 28  SRALREPYMDEIFHLPQAQRYCEGRFSLSQWDPMITTLPGLYLVSVGVVKPASWILGW-- 87

Query: 82  TRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLDDKRATTY-----AVILA 141
                S    CSI +LR +N + +V    L+Y +  +++P   +K +++       + LA
Sbjct: 88  -----SEHVVCSIGMLRFVNLLFSVGNFYLLYLLFRKIQPR--NKASSSIQRILSTLTLA 147

Query: 142 LYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIRQTNVIWMLFVAC 201
           ++P  +FF FLYYT+  S+   L  YL CL  N+  SAL G    + RQTN+IW  F  C
Sbjct: 148 VFPTLYFFNFLYYTEAGSVFFTLFAYLMCLYGNHRTSALLGFCGFMFRQTNIIWAAF--C 207

Query: 202 SGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFSPIKDVGKQVPQS 261
           +G I                         +  K S   K  L+K+K        +++P +
Sbjct: 208 AGHI-------------------------IAQKCSEAWKTELQKKK-------EERLPPA 267

Query: 262 TRFSSINQKLGLMDEIRAMI--LTMWRMKFKLLVSFF----PFVIVLMAFFAFVRWNGSI 321
                     G + E+R ++  L M+ M  K L   F    P++++L+AFF FV  NG I
Sbjct: 268 K---------GPLSELRRVLQFLLMYSMSLKNLSMLFLLTWPYMLLLLAFFVFVVVNGGI 327

Query: 322 VLGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFFQVFIAL 381
           V+G   S  A   F   + F  F A    P   + ++V       WK R     Q  +  
Sbjct: 328 VVGDRSSHEACLHFPQLFYFFSFTAFFSFPHLLSPTKVKTFLSLVWKRR----VQFSVIT 387

Query: 382 LAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSIIKILGK 441
           L     V  F+  H YLLADNRHY FY+W++V   H   KYLLVPAY+ + +++   L K
Sbjct: 388 LVSVFLVWKFTYVHKYLLADNRHYTFYVWKRVFQRHEIVKYLLVPAYMFAGWAVADSL-K 447

Query: 442 SQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL------- 501
           S+   W + +F+   A  VP  L+EFRYF  P+           RLN PL  +       
Sbjct: 448 SKSIFWNLMFFVCLVASTVPQKLLEFRYFILPYIIY--------RLNMPLPPISRLVCEL 474

Query: 502 -LYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
             Y  +N  T ++FL + F W +    QRF+W
Sbjct: 508 GCYAVVNFLTFYIFLNKTFQWSDSHDIQRFMW 474

BLAST of PI0021042 vs. ExPASy Swiss-Prot
Match: Q5I7T1 (Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG10B PE=1 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 8.1e-51
Identity = 166/503 (33.00%), Postives = 235/503 (46.72%), Query Frame = 0

Query: 22  NRILPNSYMDEIFHVPQAQKYCNGNF--RSWDPMITTPPGLYYLSLAHVASLFPGI-VFT 81
           +R L   YMDEIFH+PQAQ+YC G+F    WDPMITT PGLY +S+  V    P I +F 
Sbjct: 28  SRALREPYMDEIFHLPQAQRYCEGHFSLSQWDPMITTLPGLYLVSVGVVK---PAIWIF- 87

Query: 82  RAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLDDKRATTY-----AVILAL 141
             A S    CSI +LR +N + +V    L+Y +  +++P   +K A++       + LA+
Sbjct: 88  --AWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFHKVQPR--NKAASSIQRVLSTLTLAV 147

Query: 142 YPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIRQTNVIWMLFVACS 201
           +P  +FF FLYYT+  S+   L  YL CL  N+  SA  G    + RQTN+IW +F A +
Sbjct: 148 FPTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGFCGFMFRQTNIIWAVFCAGN 207

Query: 202 GVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFSPIKDVGKQVPQST 261
            +       +   +Q  E+                         +  PIK          
Sbjct: 208 VIAQKLTEAWKTELQKKED-------------------------RLPPIK---------- 267

Query: 262 RFSSINQKLGLMDEIRAMI--LTMWRMKFKLLVSFF----PFVIVLMAFFAFVRWNGSIV 321
                    G   E R ++  L  + M FK L   F    P++++   F AFV  NG IV
Sbjct: 268 ---------GPFAEFRKILQFLLAYSMSFKNLSMLFCLTWPYILLGFLFCAFVVVNGGIV 327

Query: 322 LGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFFQVFIALL 381
           +G   S  A   F   + F  F      P   + S++       WK   L      +  L
Sbjct: 328 IGDRSSHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKHGIL----FLVVTL 387

Query: 382 AGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSIIKILGKS 441
                V  F+ AH YLLADNRHY FY+W++V   +   KYLLVPAYI + +SI   L KS
Sbjct: 388 VSVFLVWKFTYAHKYLLADNRHYTFYVWKRVFQRYAILKYLLVPAYIFAGWSIADSL-KS 447

Query: 442 QRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALLYIALNAF 501
           +   W + +F+    V+VP  L+EFRYF  P+    L+  +            Y  +N  
Sbjct: 448 KPIFWNLMFFICLFIVIVPQKLLEFRYFILPYVIYRLNITLPPTSRLVCELSCYAIVNFI 473

Query: 502 TMFMFLFRPFYWENETGKQRFIW 508
           T ++FL + F W N    QRF+W
Sbjct: 508 TFYIFLNKTFQWPNSQDIQRFMW 473

BLAST of PI0021042 vs. ExPASy TrEMBL
Match: A0A0A0L276 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G296040 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.8e-264
Identity = 476/511 (93.15%), Postives = 487/511 (95.30%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRIAVAAIVTLWVVPISILVNRILP+SYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL
Sbjct: 1   MGRIAVAAIVTLWVVPISILVNRILPDSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFPG VFTR APSFSAACSIQILRSINGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPGTVFTRVAPSFSAACSIQILRSINGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           +KRAT YAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR
Sbjct: 121 EKRATIYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTNVIWMLFVACSGVIDTT+TF+DD+IQMHENDV VEESSSLIAKGSL SKANLRKRKFS
Sbjct: 181 QTNVIWMLFVACSGVIDTTMTFHDDNIQMHENDV-VEESSSLIAKGSLNSKANLRKRKFS 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMW MKF+LLVSFFPFV+VLMAF AFV
Sbjct: 241 RTKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWLMKFQLLVSFFPFVVVLMAFVAFV 300

Query: 301 RWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360
           RWNGSIVLG+    T SL  F     FGLF+ALLMAPMHCN SQVIDLFHSFWKGRPLSF
Sbjct: 301 RWNGSIVLGAKEAHTVSLH-FAQIMYFGLFSALLMAPMHCNPSQVIDLFHSFWKGRPLSF 360

Query: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFS 420
           FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKV+NAHWSSKYLLVPAYICSWFS
Sbjct: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVVNAHWSSKYLLVPAYICSWFS 420

Query: 421 IIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL 480
           IIKILG+SQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNW LVAL
Sbjct: 421 IIKILGESQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWSLVAL 480

Query: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           LYIALNAFTMFMFLFRPFYWENETGKQRFIW
Sbjct: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 509

BLAST of PI0021042 vs. ExPASy TrEMBL
Match: A0A1S3BJ26 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103490227 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.1e-263
Identity = 472/511 (92.37%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRIAVAAIVTLWVVPISILVNRI+P+SYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL
Sbjct: 1   MGRIAVAAIVTLWVVPISILVNRIVPDSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASL+PGIVFTR APSFS ACSIQILRSINGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLYPGIVFTRVAPSFSPACSIQILRSINGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DKRA  YAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAV IR
Sbjct: 121 DKRAMIYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVFIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTNVIWMLFVACSGVIDTTLTF+DD+IQMHENDV+VEESSSLIAKGSLTSKANLRKRKFS
Sbjct: 181 QTNVIWMLFVACSGVIDTTLTFHDDNIQMHENDVVVEESSSLIAKGSLTSKANLRKRKFS 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KDVGKQVPQ TRFSSINQKLGL+DEIRAMILTMWRM+FKLLVSFFPFVIVL+AF AFV
Sbjct: 241 RTKDVGKQVPQFTRFSSINQKLGLLDEIRAMILTMWRMRFKLLVSFFPFVIVLIAFVAFV 300

Query: 301 RWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360
            WNGSIVLG+      SL  F     FGLF+ALLMAPMHCNLSQVIDLFHSFWKGRPLSF
Sbjct: 301 CWNGSIVLGAKEEHAVSLH-FAQIMYFGLFSALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360

Query: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFS 420
           F VFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLL+PAYICSWFS
Sbjct: 361 FLVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLIPAYICSWFS 420

Query: 421 IIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL 480
           IIKILG+SQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNW LVAL
Sbjct: 421 IIKILGESQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWSLVAL 480

Query: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           LYIALNAFTMFMFLFRPFYWENETGKQRFIW
Sbjct: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 510

BLAST of PI0021042 vs. ExPASy TrEMBL
Match: A0A6J1HUC4 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111466254 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.7e-238
Identity = 426/510 (83.53%), Postives = 461/510 (90.39%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRI VAAIV+LWVVPISILV+RI+P+ YMDEIFH+PQAQKYC+GNFRSWDPMITTPPGL
Sbjct: 1   MGRIVVAAIVSLWVVPISILVDRIVPDPYMDEIFHIPQAQKYCSGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFP  +FT+AAPSFS ACS++ILRS NGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPATIFTQAAPSFSEACSVRILRSTNGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DK+ATTYAVIL+LYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYW SALFGIFAVVIR
Sbjct: 121 DKKATTYAVILSLYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWSSALFGIFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTN+IWMLFVACS VIDTTLT +D +IQMHENDVMVEE + L AKGS  S+ANLRKRKF+
Sbjct: 181 QTNIIWMLFVACSAVIDTTLTCHDHNIQMHENDVMVEEGNPLNAKGSPISRANLRKRKFN 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KD GKQ   STR S I+QK GL+ EIRAMILTMWRMKFKLLVSF PFVIVLMAF AFV
Sbjct: 241 TTKDAGKQFRHSTRSSLIDQKSGLLGEIRAMILTMWRMKFKLLVSFCPFVIVLMAFVAFV 300

Query: 301 RWNGSIVLGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFF 360
           RWNGSIVLG+  + +    F     FGLF+AL+MAPMHCN S+VID+FHS WKGRPLSF 
Sbjct: 301 RWNGSIVLGAKEAHAVSLHFAQIMYFGLFSALVMAPMHCNPSRVIDVFHSLWKGRPLSFL 360

Query: 361 QVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI 420
           QVFIALLAGFI V YFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYI SWFSI
Sbjct: 361 QVFIALLAGFIFVHYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYIYSWFSI 420

Query: 421 IKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALL 480
             ILGKS+RKIWVVAYFLATAAVLVPAPLIEFRYFTTPF+FL+L+SQMNGRLNW L+AL+
Sbjct: 421 FNILGKSKRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFFFLILNSQMNGRLNWLLIALV 480

Query: 481 YIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           YIAL+AFTMFMFLFRPFYWENETGKQRF+W
Sbjct: 481 YIALDAFTMFMFLFRPFYWENETGKQRFLW 510

BLAST of PI0021042 vs. ExPASy TrEMBL
Match: A0A6J1EVG0 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111438157 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.5e-237
Identity = 422/510 (82.75%), Postives = 460/510 (90.20%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRI VAAIV+LWVVPISILVNRI+P+ YMDEIFH+PQAQKYC+GNFRSWDPMITTPPGL
Sbjct: 1   MGRIVVAAIVSLWVVPISILVNRIVPDPYMDEIFHIPQAQKYCSGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFP  +FT+AAPSFS ACS++ILRS NGI AVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPATIFTQAAPSFSEACSVRILRSTNGIFAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DK+ATTYAVIL+LYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYW SALFGIFAVVIR
Sbjct: 121 DKKATTYAVILSLYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWSSALFGIFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTN+IWMLFVACS VIDTTLT +DD+IQMHENDVMVEES+ L AKGS  S+ANLRKRKF 
Sbjct: 181 QTNIIWMLFVACSAVIDTTLTCHDDNIQMHENDVMVEESNPLNAKGSPISRANLRKRKFI 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KD GKQ   STR S I+QK GL+DEIRA+ILT+W MKFKLLVSF PFVIVLMAF AFV
Sbjct: 241 TTKDAGKQFRHSTRSSLIDQKSGLLDEIRALILTVWHMKFKLLVSFCPFVIVLMAFVAFV 300

Query: 301 RWNGSIVLGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFF 360
           RWNGSIVLG+  + +    F     FGLF+AL+MAPMHCN SQVID+FHS WKGRPLSF 
Sbjct: 301 RWNGSIVLGAKEAHAVSLHFAQIMYFGLFSALVMAPMHCNPSQVIDVFHSLWKGRPLSFL 360

Query: 361 QVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI 420
           QVFIALLAGFI V YFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYI SWFSI
Sbjct: 361 QVFIALLAGFIFVHYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYIYSWFSI 420

Query: 421 IKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALL 480
             ILGKS+RKIWV+AYFLATAAVLVPAPLIEFRYFTTPF+FL+L+S++NGRLNW L+AL+
Sbjct: 421 FNILGKSKRKIWVLAYFLATAAVLVPAPLIEFRYFTTPFFFLILNSELNGRLNWLLIALV 480

Query: 481 YIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           YIAL+AFTM MFLFRPF+WENETGKQRF+W
Sbjct: 481 YIALDAFTMIMFLFRPFHWENETGKQRFLW 510

BLAST of PI0021042 vs. ExPASy TrEMBL
Match: A0A6J1CRK7 (Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Momordica charantia OX=3673 GN=LOC111013674 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 7.2e-236
Identity = 423/511 (82.78%), Postives = 459/511 (89.82%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRIAVA IV+LWVVPISILVNRI+P+ YMDEIFH+PQAQKYC+GNFRSWDPMITTPPGL
Sbjct: 1   MGRIAVAVIVSLWVVPISILVNRIVPDPYMDEIFHIPQAQKYCSGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAH+ASLFP +VFT+A  SFS ACS QILRS NGILAVLCSVLVY+IIS LRPNLD
Sbjct: 61  YYLSLAHIASLFPAVVFTQAGSSFSEACSTQILRSTNGILAVLCSVLVYDIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DK+ATTYAVI ALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYW SALFGIFAVVIR
Sbjct: 121 DKKATTYAVIFALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWSSALFGIFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTN+IWMLFVAC GVIDTTLT +DD++Q+HEND +V+ESS L AKGSL S ANLRKRKF 
Sbjct: 181 QTNIIWMLFVACCGVIDTTLTCHDDNVQIHEND-LVQESSPLNAKGSLVSSANLRKRKFY 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             K  GKQ P+STRFSSIN K GLMDE+R+MILTMWRMK +LLVSF PF+IVLMAF AFV
Sbjct: 241 RTKVAGKQFPRSTRFSSINLKSGLMDEVRSMILTMWRMKSELLVSFSPFLIVLMAFVAFV 300

Query: 301 RWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360
           RWNGSIVLG+    T SL  F     FGLF+AL+ AP+H NLS+VIDLFHSFWKGRPLS 
Sbjct: 301 RWNGSIVLGAKEAHTVSLH-FAQIMYFGLFSALVTAPLHYNLSEVIDLFHSFWKGRPLSC 360

Query: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFS 420
           FQVFI LLAGFISV YFSIAHPYLLADNRHYPFYLWRKVIN +WSSKYLLVPAY+CSWFS
Sbjct: 361 FQVFIFLLAGFISVHYFSIAHPYLLADNRHYPFYLWRKVINTNWSSKYLLVPAYVCSWFS 420

Query: 421 IIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL 480
           I  ILGK +RKIWV+AYF+ATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRL+W L+AL
Sbjct: 421 IFNILGKFRRKIWVLAYFVATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLSWLLIAL 480

Query: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           L+IALNAFTM MFLFRPFYWENE+GKQRFIW
Sbjct: 481 LFIALNAFTMIMFLFRPFYWENESGKQRFIW 509

BLAST of PI0021042 vs. NCBI nr
Match: XP_011653629.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucumis sativus] >XP_011653630.1 dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucumis sativus] >KGN54251.1 hypothetical protein Csa_017996 [Cucumis sativus])

HSP 1 Score: 920.6 bits (2378), Expect = 5.8e-264
Identity = 476/511 (93.15%), Postives = 487/511 (95.30%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRIAVAAIVTLWVVPISILVNRILP+SYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL
Sbjct: 1   MGRIAVAAIVTLWVVPISILVNRILPDSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFPG VFTR APSFSAACSIQILRSINGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPGTVFTRVAPSFSAACSIQILRSINGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           +KRAT YAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR
Sbjct: 121 EKRATIYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTNVIWMLFVACSGVIDTT+TF+DD+IQMHENDV VEESSSLIAKGSL SKANLRKRKFS
Sbjct: 181 QTNVIWMLFVACSGVIDTTMTFHDDNIQMHENDV-VEESSSLIAKGSLNSKANLRKRKFS 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMW MKF+LLVSFFPFV+VLMAF AFV
Sbjct: 241 RTKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWLMKFQLLVSFFPFVVVLMAFVAFV 300

Query: 301 RWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360
           RWNGSIVLG+    T SL  F     FGLF+ALLMAPMHCN SQVIDLFHSFWKGRPLSF
Sbjct: 301 RWNGSIVLGAKEAHTVSLH-FAQIMYFGLFSALLMAPMHCNPSQVIDLFHSFWKGRPLSF 360

Query: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFS 420
           FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKV+NAHWSSKYLLVPAYICSWFS
Sbjct: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVVNAHWSSKYLLVPAYICSWFS 420

Query: 421 IIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL 480
           IIKILG+SQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNW LVAL
Sbjct: 421 IIKILGESQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWSLVAL 480

Query: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           LYIALNAFTMFMFLFRPFYWENETGKQRFIW
Sbjct: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 509

BLAST of PI0021042 vs. NCBI nr
Match: XP_008447877.1 (PREDICTED: dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 916.8 bits (2368), Expect = 8.4e-263
Identity = 472/511 (92.37%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRIAVAAIVTLWVVPISILVNRI+P+SYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL
Sbjct: 1   MGRIAVAAIVTLWVVPISILVNRIVPDSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASL+PGIVFTR APSFS ACSIQILRSINGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLYPGIVFTRVAPSFSPACSIQILRSINGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DKRA  YAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAV IR
Sbjct: 121 DKRAMIYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVFIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTNVIWMLFVACSGVIDTTLTF+DD+IQMHENDV+VEESSSLIAKGSLTSKANLRKRKFS
Sbjct: 181 QTNVIWMLFVACSGVIDTTLTFHDDNIQMHENDVVVEESSSLIAKGSLTSKANLRKRKFS 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KDVGKQVPQ TRFSSINQKLGL+DEIRAMILTMWRM+FKLLVSFFPFVIVL+AF AFV
Sbjct: 241 RTKDVGKQVPQFTRFSSINQKLGLLDEIRAMILTMWRMRFKLLVSFFPFVIVLIAFVAFV 300

Query: 301 RWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360
            WNGSIVLG+      SL  F     FGLF+ALLMAPMHCNLSQVIDLFHSFWKGRPLSF
Sbjct: 301 CWNGSIVLGAKEEHAVSLH-FAQIMYFGLFSALLMAPMHCNLSQVIDLFHSFWKGRPLSF 360

Query: 361 FQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFS 420
           F VFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLL+PAYICSWFS
Sbjct: 361 FLVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLIPAYICSWFS 420

Query: 421 IIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVAL 480
           IIKILG+SQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNW LVAL
Sbjct: 421 IIKILGESQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWSLVAL 480

Query: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           LYIALNAFTMFMFLFRPFYWENETGKQRFIW
Sbjct: 481 LYIALNAFTMFMFLFRPFYWENETGKQRFIW 510

BLAST of PI0021042 vs. NCBI nr
Match: XP_038877648.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Benincasa hispida])

HSP 1 Score: 867.1 bits (2239), Expect = 7.6e-248
Identity = 441/510 (86.47%), Postives = 469/510 (91.96%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRIAVAAIV+LWVVPISILVNRILP+ YMDEIFHVPQAQKYC+GNFRSWDPMITTPPGL
Sbjct: 1   MGRIAVAAIVSLWVVPISILVNRILPDPYMDEIFHVPQAQKYCSGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFP IVFTRAAPSFS ACS+QILRS NGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPAIVFTRAAPSFSEACSVQILRSTNGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DK+ATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLS LFG+FAVVIR
Sbjct: 121 DKKATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSTLFGVFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTNV+WMLFVACSG+IDTTLT +DD+IQMHEN+V+VEESS L AKG+  S+ NLRKRK +
Sbjct: 181 QTNVVWMLFVACSGIIDTTLTCHDDTIQMHENNVVVEESSPLNAKGNFISRVNLRKRKLN 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KD G+Q  QSTRFSSINQKLGLMDEIRAM+LTMW MKFKLLVSF PFVI+LMAF AFV
Sbjct: 241 RTKDEGEQFTQSTRFSSINQKLGLMDEIRAMLLTMWHMKFKLLVSFCPFVIMLMAFVAFV 300

Query: 301 RWNGSIVLGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFF 360
           RWNGSIVLG+  + +    F     FGLF+AL+MAP HCNLSQ++DLFHSFWKGRPLSF 
Sbjct: 301 RWNGSIVLGAKEAHAVSLHFAQILYFGLFSALVMAPRHCNLSQLLDLFHSFWKGRPLSFL 360

Query: 361 QVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI 420
           QVFIALLAGFISV YFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI
Sbjct: 361 QVFIALLAGFISVHYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI 420

Query: 421 IKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALL 480
           I IL KSQRKIWV+AYFLATAAVLVPAPLIEFRYFTTPFYFLMLH QMNGRL+W LVALL
Sbjct: 421 IDILRKSQRKIWVLAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHCQMNGRLSWLLVALL 480

Query: 481 YIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           YI+LNAFTMFMFLFRPFYWENETG QRFIW
Sbjct: 481 YISLNAFTMFMFLFRPFYWENETGNQRFIW 510

BLAST of PI0021042 vs. NCBI nr
Match: XP_022966629.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucurbita maxima])

HSP 1 Score: 834.3 bits (2154), Expect = 5.5e-238
Identity = 426/510 (83.53%), Postives = 461/510 (90.39%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRI VAAIV+LWVVPISILV+RI+P+ YMDEIFH+PQAQKYC+GNFRSWDPMITTPPGL
Sbjct: 1   MGRIVVAAIVSLWVVPISILVDRIVPDPYMDEIFHIPQAQKYCSGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFP  +FT+AAPSFS ACS++ILRS NGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPATIFTQAAPSFSEACSVRILRSTNGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DK+ATTYAVIL+LYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYW SALFGIFAVVIR
Sbjct: 121 DKKATTYAVILSLYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWSSALFGIFAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTN+IWMLFVACS VIDTTLT +D +IQMHENDVMVEE + L AKGS  S+ANLRKRKF+
Sbjct: 181 QTNIIWMLFVACSAVIDTTLTCHDHNIQMHENDVMVEEGNPLNAKGSPISRANLRKRKFN 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KD GKQ   STR S I+QK GL+ EIRAMILTMWRMKFKLLVSF PFVIVLMAF AFV
Sbjct: 241 TTKDAGKQFRHSTRSSLIDQKSGLLGEIRAMILTMWRMKFKLLVSFCPFVIVLMAFVAFV 300

Query: 301 RWNGSIVLGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFF 360
           RWNGSIVLG+  + +    F     FGLF+AL+MAPMHCN S+VID+FHS WKGRPLSF 
Sbjct: 301 RWNGSIVLGAKEAHAVSLHFAQIMYFGLFSALVMAPMHCNPSRVIDVFHSLWKGRPLSFL 360

Query: 361 QVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI 420
           QVFIALLAGFI V YFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYI SWFSI
Sbjct: 361 QVFIALLAGFIFVHYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYIYSWFSI 420

Query: 421 IKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALL 480
             ILGKS+RKIWVVAYFLATAAVLVPAPLIEFRYFTTPF+FL+L+SQMNGRLNW L+AL+
Sbjct: 421 FNILGKSKRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFFFLILNSQMNGRLNWLLIALV 480

Query: 481 YIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           YIAL+AFTMFMFLFRPFYWENETGKQRF+W
Sbjct: 481 YIALDAFTMFMFLFRPFYWENETGKQRFLW 510

BLAST of PI0021042 vs. NCBI nr
Match: XP_023518927.1 (dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.2 bits (2151), Expect = 1.2e-237
Identity = 426/510 (83.53%), Postives = 460/510 (90.20%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MGRI VAAIV+LWVVPISILVNRI+P+ YMDEIFH+PQAQKYC+GNFRSWDPMITTPPGL
Sbjct: 1   MGRIVVAAIVSLWVVPISILVNRIVPDPYMDEIFHIPQAQKYCSGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPGIVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNLD 120
           YYLSLAHVASLFP  +FT+AAPSFS ACS++ILRS NGILAVLCSVLVYEIIS LRPNLD
Sbjct: 61  YYLSLAHVASLFPATIFTQAAPSFSEACSVRILRSTNGILAVLCSVLVYEIISHLRPNLD 120

Query: 121 DKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVIR 180
           DK+ATTYAVIL+LYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYW SALFGI AVVIR
Sbjct: 121 DKKATTYAVILSLYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWSSALFGISAVVIR 180

Query: 181 QTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKFS 240
           QTN+IWMLFVACS VIDTTLT +DD+IQMHENDVMVEES+ L AKGS  S+ANLRKRKF+
Sbjct: 181 QTNIIWMLFVACSAVIDTTLTCHDDNIQMHENDVMVEESNPLNAKGSPISRANLRKRKFN 240

Query: 241 PIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAFV 300
             KD GKQ   STR SSI+QK GLMDEIRAMILT+WRMKFKLLVSF PFVIVL AF AFV
Sbjct: 241 STKDAGKQFRHSTRSSSIDQKSGLMDEIRAMILTIWRMKFKLLVSFCPFVIVLTAFVAFV 300

Query: 301 RWNGSIVLGSTCSLSA---FCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLSFF 360
           RWNGSIVLG+  + +    F     FGLF+AL+MAP HCN SQVID+FHS WKGRPLSF 
Sbjct: 301 RWNGSIVLGAKEAHAVSLHFAQIMYFGLFSALVMAPTHCNPSQVIDVFHSLWKGRPLSFL 360

Query: 361 QVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWFSI 420
           QVFIALLAGFI V YFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYI SWFSI
Sbjct: 361 QVFIALLAGFIFVHYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYIYSWFSI 420

Query: 421 IKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHSQMNGRLNWPLVALL 480
             ILGKS+RKIWVVAYFLATAAVLVPAPLIEFRYFTTPF+FL+L+SQMNGRLNW L+AL+
Sbjct: 421 FNILGKSKRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFFFLILNSQMNGRLNWLLIALV 480

Query: 481 YIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           YI L+AFTM MFLFRPF+WENETGKQRF+W
Sbjct: 481 YITLDAFTMIMFLFRPFHWENETGKQRFLW 510

BLAST of PI0021042 vs. TAIR 10
Match: AT5G02410.1 (DIE2/ALG10 family )

HSP 1 Score: 563.5 bits (1451), Expect = 1.7e-160
Identity = 294/514 (57.20%), Postives = 367/514 (71.40%), Query Frame = 0

Query: 1   MGRIAVAAIVTLWVVPISILVNRILPNSYMDEIFHVPQAQKYCNGNFRSWDPMITTPPGL 60
           MG++AVAAI +LWV+P+SI+VN I+P  YMDEIFHVPQAQ+YCNGNFRSWDPMITTPPGL
Sbjct: 1   MGKLAVAAITSLWVIPMSIIVNHIVPEPYMDEIFHVPQAQQYCNGNFRSWDPMITTPPGL 60

Query: 61  YYLSLAHVASLFPG-IVFTRAAPSFSAACSIQILRSINGILAVLCSVLVYEIISQLRPNL 120
           YYLSLAHVASLFPG ++    + SFS ACS  +LRS N + AVLC VLVYEII  L PNL
Sbjct: 61  YYLSLAHVASLFPGMLLMENTSQSFSEACSTSVLRSTNAVSAVLCGVLVYEIIRFLGPNL 120

Query: 121 DDKRATTYAVILALYPLHWFFTFLYYTDVASLTAVLAMYLACLKKNYWLSALFGIFAVVI 180
            D++AT  A++++LYPLHWFFTFLYYTDVASLTAVLAMYL CLK+ Y LSALFG  AV I
Sbjct: 121 SDRKATFMALVMSLYPLHWFFTFLYYTDVASLTAVLAMYLTCLKRRYVLSALFGTLAVFI 180

Query: 181 RQTNVIWMLFVACSGVIDTTLTFYDDSIQMHENDVMVEESSSLIAKGSLTSKANLRKRKF 240
           RQTNV+WMLFVACSG++D TL   D S Q  + +V  E   S   KG+ T ++NLRKRK 
Sbjct: 181 RQTNVVWMLFVACSGILDFTL---DSSKQKGKQEVNQELHQSSNKKGA-TLRSNLRKRKS 240

Query: 241 SPIKDVGKQVPQSTRFSSINQKLGLMDEIRAMILTMWRMKFKLLVSFFPFVIVLMAFFAF 300
               D            S      L+ +I  +I T W +K+++L+ F PF+ V++AF  F
Sbjct: 241 DISSDTSDPFNHGQTVPSTEDTSDLVYDIYTVISTSWNLKWRILIKFSPFIFVVVAFGIF 300

Query: 301 VRWNGSIVLGS----TCSLSAFCTDYVFGLFAALLMAPMHCNLSQVIDLFHSFWKGRPLS 360
           + WNG IVLG+      SL  F     F L +AL  AP+H +++Q+   FH   +   LS
Sbjct: 301 ILWNGGIVLGAKEAHVVSLH-FAQIMYFSLVSALFTAPLHFSVNQLRHQFHQLHRNWSLS 360

Query: 361 FFQVFIALLAGFISVQYFSIAHPYLLADNRHYPFYLWRKVINAHWSSKYLLVPAYICSWF 420
                +AL+AGF+SV +FS+AHPYLLADNRHYPFYLWRK+INAHW  KY+LVP Y+ SWF
Sbjct: 361 LILTLVALVAGFVSVHFFSLAHPYLLADNRHYPFYLWRKIINAHWLMKYILVPVYVYSWF 420

Query: 421 SIIKILGKSQRKIWVVAYFLATAAVLVPAPLIEFRYFTTPFYFLMLHS--QMNGRLNWPL 480
           SI+ +L K++R+ W++ YFLAT  VLVP PLIEFRY+T PFY  MLHS  + +    W L
Sbjct: 421 SILTLLAKTRRQTWILVYFLATCGVLVPTPLIEFRYYTIPFYLFMLHSCVRSSSFATWLL 480

Query: 481 VALLYIALNAFTMFMFLFRPFYWENETGKQRFIW 508
           +  +++++N FTM MFLFRPF W +E G QRFIW
Sbjct: 481 IGTIFVSINVFTMAMFLFRPFKWSHEDGVQRFIW 509

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6382.4e-15957.20Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Arabidop... [more]
Q3UGP87.1e-5534.98Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS... [more]
Q5BKT45.1e-5333.20Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Homo sap... [more]
O887881.5e-5233.79Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS... [more]
Q5I7T18.1e-5133.00Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS... [more]
Match NameE-valueIdentityDescription
A0A0A0L2762.8e-26493.15Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucumis ... [more]
A0A1S3BJ264.1e-26392.37Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucumis ... [more]
A0A6J1HUC42.7e-23883.53Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbit... [more]
A0A6J1EVG06.5e-23782.75Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Cucurbit... [more]
A0A6J1CRK77.2e-23682.78Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase OS=Momordic... [more]
Match NameE-valueIdentityDescription
XP_011653629.15.8e-26493.15dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucumis sa... [more]
XP_008447877.18.4e-26392.37PREDICTED: dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase ... [more]
XP_038877648.17.6e-24886.47dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Benincasa ... [more]
XP_022966629.15.5e-23883.53dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucurbita ... [more]
XP_023518927.11.2e-23783.53dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT5G02410.11.7e-16057.20DIE2/ALG10 family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016900Alpha-2-glucosyltransferase Alg10PIRSFPIRSF028810Alg10coord: 1..507
e-value: 7.1E-137
score: 454.8
IPR016900Alpha-2-glucosyltransferase Alg10PFAMPF04922DIE2_ALG10coord: 26..461
e-value: 1.0E-114
score: 383.9
IPR016900Alpha-2-glucosyltransferase Alg10PANTHERPTHR12989ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10coord: 3..507
NoneNo IPR availablePANTHERPTHR12989:SF10DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATEDcoord: 3..507

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021042.1PI0021042.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006488 dolichol-linked oligosaccharide biosynthetic process
biological_process GO:0048366 leaf development
biological_process GO:0009651 response to salt stress
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0106073 dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity