PI0021021 (gene) Melon (PI 482460) v1

Overview


Name	PI0021021
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Transcription initiation factor IIF subunit alpha
Location	chr04: 18776801 .. 18780227 (+)
RNA-Seq Expression	PI0021021
Synteny	PI0021021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTTCGATTTGCTCCTGAAGCCATCGTGCAGTGGATGTGGATCCACGACGGAGTTGTATGGGAGCAATTGCAAGCACATGACATTGTGTTTGACCTGTGGCAAAACTATGGCGGAGAACAAGGGCAAATGTTATGATTGTGGGGCTACTGTAACTCGCTTAATAAGAGTTCGTTCTTTACCTCGTTGTTCTTAAAGAGCGTAATGATTTGAATGTGTTAAATTTGTACTCATGTGATTAGGGGTTCTTATTTACGACAGATTTAAGAATTTTCATAATAGCGTGGATTGAGTACTGTCTGCCTTCTCTTTCTGAGAGTGCATGATGAACTATATTTTAGGAATACAATGTTCGAGCAAGTGCAAGTAGCGACAAGAACTACTTCATCGGTAGGTTCATGAGTGGGCTGCCGAACTTTTCGAAGAAGAGAAATGCTGAAAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAACTTACCGATGCCCTTCGGGTACTTAGTTCACTTCAATTTTGCTTCCTTTCTTTAGTTTGTCTTGGATAGTTTATTGTTTTGGAAGCCTGGATATTGGTGACTTGATATCTATTTTTCTTTTTAGAAGTTATAACTGTTAAGAGTAGCTAGGGAAAAAGTAATTGGTGCTATCTTTCAACAAAATTTAAAAGGAACACCTATGAAGCCCAAACAAATATGAATACTTTGATACGTCAATTTCTAAAAGCCTAGGAAATTAATATGCTGAGGTACACTTTTCTAAGATTCTAAAATATAGGTAAATTTAACTCAAAGGTACTAAAAAACATGTCCAAGTTAATGACAATAGTGCAAAATACAAATATACAATACAAACATTGAAATACAACACGAAAAGTAGCATTAGAGATATTTAAATACAATAATAGCAATTCGAATACAATAGAAAAGGTCAGAAAAGGAGCACTGCTAAGATAAATGAAGAACTTCATTAAATTGTTGAATATTTTTTATTTGGCTCTGTGGGGACTTAGTCCCGAAGATGGGGATTCGATGATAATATGACTTTATTTTTTAAGTTGGGGCAATATGCTGATTAGTTTTGAGTTGGGAAAGATTAGATGAGTTTTTTTATTTTATTTTAAAAAAGGTCTGCTGTGTCCCCTTCACGTATTAGAACACACGTATTCAATCTTTTAAGAAAAAAGTTCCAAATTGCATATCAGATACACATGTAGGAGTATCGGTATTTGATACGATCCGATACTGGTTCTCTGCCTCACATCAAGTATCCATGCTTCATAGGGGAACACATTTTCTTTAATGCAGATAAATGGTCCGGTTCTTGATGGACATGGAATATCATACACTTTTTTTTTGTTACTTCTTGGGCTTGTTGCCACAATAGAGTGCCTAAAATTATCCTCTTTGTATTGGTTGGAAATTCTCTATTTGTTTTACGGCTTATGTATATGTTGGGCTATATAACTACTTGTTTTGTGTTCACTATTTAATTATTACCTTGTGTTAACCATAACTCAGGCTCTCAGCTTTGACCCTGGAGATGCATTTGGTTAATATCTTGTTGCATTCATCTTTTCTTGTTTCTCTCTTCAAGTTAGTTGTTTTCTGGCATATTGCTTACCTTTTCTTTGTTTCTTTGCTTCTTTCCTTTTTAATGGTTATTTTCACTCTTGTTCTGCTTGACCCTGATCAATTTTGATTATGTTAACATAGGAAAAGTACAAGAATAGACCTTGGCTGTTGGAAGATGAAACAGGCCAATTTCAATTCCAAGGTCATCTTGAGGGCTCACAATCAGCAACATACTACTTGCTTGTATTGCAGGGAAAGGAATTTACTGCCATTCCTGCAGGTTCTTGGTATGGTTTATCTTTGAGTTAGTCTGCCATGGATCGAGTTATAGCCCTTTATTTGTTTGGCATAGTCTTCTTCATACATGTTTATTATTCTTTCCTATTCTTGGAGGCTTCTATATTGTCCAGATGAAATATTTTAAGTCATGCATGGTGGTAGCAGAAAGACTGACCTCAATTTTCAGGTACAACTTCAATAAAGTGGCACAGTACAAGCAACTTACATTGGAGGAGGCAGAAGAAAAAATGAAGAATCGAAAGAAGACGGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCATTTGGTGAAGTTGAGAAGTTTGATGACAAAGAAACTGTTGGTACTAGTGGTAGGGGACGGAGAAAAACTACGGGTGATGAGAATGAAGGCAACGTCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAGTAAGAAGGGTGGTGGTGATGATGATGATGAGGAAGGGCCTAGGGTGGTGATCATGACTTGGATGATGATGATATTGAGAAAGGTTATCTTTTCTGTTTAAATAAAAGCAAAATTTTGTTTAGGTCAAGTGCTTTACTACCATTGTCATCTCGATAGACTGTCACTTGCCATCTGTTGATTGTTTACGGGCTAGGCTGTCACCACCGTATGGGAACCAAATGCATCAAATGAGTGATTTAGATTTGGGGACAGCATTAGATTAGCAAATGCTTTTGTTCTCCAGTAAGATTCACATTATCACCATGTTTTTTTAATAGCGTTTGACAAGGAAACACTTTCTTGTATCCATGTATGTGAAATTTGGCTGTTAATTCATATGATGGGCATTGAGCTATGTTAACCTGTCTGTCCAACTTTCTAGGGGATGATTGGGAGCACGAAGAGATTTTTACTGATGATGATGAAACTACTGCTCCAGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCTGCACCTCCTGAAATCAAGCAGGTTGTCTATTCCTAATGAAAAAAGAAAAAAAAAAAATTCGCTGATATATATCACTTCATCCATTACTTGAAACAATTGTTTATATTTAACAGTTGAAGTAACTGTATATGTTTTTCTCCAAAGGTGCTGGAAAGGGTGGTAAGAAATGAAATATTAGAATGTTTTTGTAAGAATAAAAAGAAAAGGATCAAGACAAATGGAATGAAAAGACAAGCTAATGTAGGATGGGCGATGGAAAATTGGAAAGGTGGAGAGGAGATTATGAATGACCATAATTTTTTCTGAAAGTAGTCACATGCACATGTACAGGTGTGATTAAATGGCAACAGTATATCATTTTCCACTGAACCTTAACATCCCCTCAAGTTCTGGTTGACATCTTGAATTGTCTGAAGCCCTTTTTATTCTTTTTAATATTTTATTTTATTTCTATGTAGAAACCTTTCAAGTCAAAGATATCACATCTCGGATATAAATGGCTTGTTTATATGACTAAGCTTACTATTTCAGTAAATTATGGACTCTTTGTAGGATGAAGAAGATGAGGATGA

mRNA sequence

ATGTCGTTCGATTTGCTCCTGAAGCCATCGTGCAGTGGATGTGGATCCACGACGGAGTTGTATGGGAGCAATTGCAAGCACATGACATTGTGTTTGACCTGTGGCAAAACTATGGCGGAGAACAAGGGCAAATGTTATGATTGTGGGGCTACTGTAACTCGCTTAATAAGAGAATACAATGTTCGAGCAAGTGCAAGTAGCGACAAGAACTACTTCATCGGTAGGTTCATGAGTGGGCTGCCGAACTTTTCGAAGAAGAGAAATGCTGAAAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAACTTACCGATGCCCTTCGGGAAAAGTACAAGAATAGACCTTGGCTGTTGGAAGATGAAACAGGCCAATTTCAATTCCAAGGTCATCTTGAGGGCTCACAATCAGCAACATACTACTTGCTTGTATTGCAGGGAAAGGAATTTACTGCCATTCCTGCAGGTTCTTGGTACAACTTCAATAAAGTGGCACAGTACAAGCAACTTACATTGGAGGAGGCAGAAGAAAAAATGAAGAATCGAAAGAAGACGGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCATTTGGTGAAGTTGAGAAGTTTGATGACAAAGAAACTGTTGGTACTAGTGGTAGGGGACGGAGAAAAACTACGGGTGATGAGAATGAAGGCAACGTCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAGTAAGAAGGGTGGTGGTGATGATGATGATGAGGAAGGGCCTAGGGTGGTGATCATGACTTGGATGATGATGATATTGAGAAAGCACGAAGAGATTTTTACTGATGATGATGAAACTACTGCTCCAGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCTGCACCTCCTGAAATCAAGCAGGTGCTGGAAAGGGTGGTAAGAAATGAAATATTAGAATGTTTTTGTAAGAATAAAAAGAAAAGGATCAAGACAAATGGAATGAAAAGACAAGCTAATGTAGGATGGGCGATGGAAAATTGGAAAGGTGGAGAGGAGATTATGAATGACCATAATTTTTTCTGAAAGTAGTCACATGCACATGTACAGGATGAAGAAGATGAGGATGA

Coding sequence (CDS)

ATGTCGTTCGATTTGCTCCTGAAGCCATCGTGCAGTGGATGTGGATCCACGACGGAGTTGTATGGGAGCAATTGCAAGCACATGACATTGTGTTTGACCTGTGGCAAAACTATGGCGGAGAACAAGGGCAAATGTTATGATTGTGGGGCTACTGTAACTCGCTTAATAAGAGAATACAATGTTCGAGCAAGTGCAAGTAGCGACAAGAACTACTTCATCGGTAGGTTCATGAGTGGGCTGCCGAACTTTTCGAAGAAGAGAAATGCTGAAAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAACTTACCGATGCCCTTCGGGAAAAGTACAAGAATAGACCTTGGCTGTTGGAAGATGAAACAGGCCAATTTCAATTCCAAGGTCATCTTGAGGGCTCACAATCAGCAACATACTACTTGCTTGTATTGCAGGGAAAGGAATTTACTGCCATTCCTGCAGGTTCTTGGTACAACTTCAATAAAGTGGCACAGTACAAGCAACTTACATTGGAGGAGGCAGAAGAAAAAATGAAGAATCGAAAGAAGACGGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCATTTGGTGAAGTTGAGAAGTTTGATGACAAAGAAACTGTTGGTACTAGTGGTAGGGGACGGAGAAAAACTACGGGTGATGAGAATGAAGGCAACGTCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAGTAAGAAGGGTGGTGGTGATGATGATGATGAGGAAGGGCCTAGGGTGGTGATCATGACTTGGATGATGATGATATTGAGAAAGCACGAAGAGATTTTTACTGATGATGATGAAACTACTGCTCCAGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCTGCACCTCCTGAAATCAAGCAGGTGCTGGAAAGGGTGGTAAGAAATGAAATATTAGAATGTTTTTGTAAGAATAAAAAGAAAAGGATCAAGACAAATGGAATGAAAAGACAAGCTAATGTAGGATGGGCGATGGAAAATTGGAAAGGTGGAGAGGAGATTATGAATGACCATAATTTTTTCTGA

Protein sequence

MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYNVRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLLEDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRGEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRKHEEIFTDDDETTAPDREEREDLAPEVPAPPEIKQVLERVVRNEILECFCKNKKKRIKTNGMKRQANVGWAMENWKGGEEIMNDHNFF

Homology

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BLAST of PI0021021 vs. ExPASy Swiss-Prot
Match: Q9SU25 (Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=3702 GN=RAP74 PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.5e-123
Identity = 235/328 (71.65%), Postives = 272/328 (82.93%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS  L L  SC GCGS ++LYGS+C+HMTLCL CG+TMA+NK KC++CG  VTRLIREYN
Sbjct: 1   MSNCLQLNTSCVGCGSQSDLYGSSCRHMTLCLKCGRTMAQNKSKCHECGTVVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRA+A +DKNYFIGRF++GLPNF  K+ +ENKWSL K+  QGRQ TDA REK KN+PW+L
Sbjct: 61  VRAAAPTDKNYFIGRFVTGLPNF--KKGSENKWSLRKDIPQGRQFTDAQREKLKNKPWIL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQ+QGHLEGSQSATYYLLV+Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQYQGHLEGSQSATYYLLVMQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTS---GRGRRKTT-GDENEGNV 240
           KNR+KTADGY+RWMMKAA+NGPA FGEV+  ++KE+ GTS   GRGR+K++ GDE EGNV
Sbjct: 181 KNRRKTADGYQRWMMKAANNGPALFGEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGNV 240

Query: 241 SDRGEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTD 300
           SDRG+EDEEEEA+RK+RLGL++K   DDDDEEGPR   +      + K     HEEIFTD
Sbjct: 241 SDRGDEDEEEEASRKSRLGLNRK-SNDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTD 300

Query: 301 DDETTAPDREERED-LAPEVPAPPEIKQ 319
           DDE    D EERED LAPE+PAPPEIKQ
Sbjct: 301 DDEAVGNDPEEREDLLAPEIPAPPEIKQ 323

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BLAST of PI0021021 vs. ExPASy Swiss-Prot
Match: Q54WV4 (General transcription factor IIF subunit 1 OS=Dictyostelium discoideum OX=44689 GN=gtf2f1 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-08
Identity = 57/204 (27.94%), Postives = 95/204 (46.57%), Query Frame = 0


Query: 114 KNRPWLLEDETGQFQFQGHLEGSQ-SATYYLLVLQGK-EFTAIPAGSWYNFNKVAQYKQL 173
           K  PW LED  G   +QG++EG+Q S+ Y+L + Q      A+P   WYNF    +++ L
Sbjct: 146 KTIPWKLEDSEGNNTYQGNVEGNQASSNYFLFMFQSDGSIKAVPCNDWYNFRPKKEFQSL 205

Query: 174 TLEEAEEKMKNRKKTADGYERWMMK------AASNGPAAFGE--VEKFDDKETVG-TSGR 233
           T EEAE+ MK + +  D +   + K      + S G  + G+  +E+ ++ E        
Sbjct: 206 TTEEAEDFMKKKNQEWDVFTSRLQKKTDPSGSGSGGSESSGKKSIEELEEAEHRNRNEDP 265

Query: 234 GRRKTTGDENEGNVSDRGEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMIL 293
            R KTT +E +   +     + E+E   ++      +   DDDD++       T+M    
Sbjct: 266 NRYKTTNEEKKKKKAPARRREREDEGNGEDGDAPDFESKFDDDDDD-------TYM---- 325

Query: 294 RKHEEIFTDDDETTAPDREEREDL 307
               +  T +DE    + EE ED+
Sbjct: 326 --DGDGLTGEDENQIEEDEEEEDV 336

[Close]

BLAST of PI0021021 vs. ExPASy Swiss-Prot
Match: O94416 (Transcription initiation factor IIF subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tfg1 PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 3.8e-05
Identity = 61/244 (25.00%), Postives = 94/244 (38.52%), Query Frame = 0


Query: 58  EYNVRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRP 117
           E N    +SS+ N        G  N  +         + K   Q R+L     ++ +  P
Sbjct: 96  EQNAEQGSSSNVNIASIAPYGGAQNMKQNAFKRKTRQVVKVDPQARRL-----QEEELSP 155

Query: 118 WLLEDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAE 177
           W++ED  G+  +   +EG QS+ Y L +     F  IP   +Y FN+   ++ L+++EAE
Sbjct: 156 WIMEDFEGKNTWVSTMEGGQSSAYVLFMFSENGFKVIPTDRFYRFNQRNNFQTLSIDEAE 215

Query: 178 EKMKNRKKTADGYERWMMKAAS-------------------------NGPAAFGEVEKFD 237
            KM   KKT     RW MK  S                          GP A G  ++ D
Sbjct: 216 AKM--NKKTP--IPRWFMKKESEENLAEAGSASPMYKLKTVPNARPVTGPRASGSDDELD 275

Query: 238 DKETVGTSGRGRRKTTGDENEGNVSDRGEEDEE--EEAARKNRLGLSKKGGGDDD---DE 272
             E              D+ E  + +  EED +  ++  +K  L  +  G  D D   +E
Sbjct: 276 YDEEF-----------ADDEEAPIMEGNEEDNKKLKDKIKKEMLTANLFGEADQDVDLEE 319

[Close]

BLAST of PI0021021 vs. ExPASy TrEMBL
Match: A0A1S4DWR2 (Transcription initiation factor IIF subunit alpha OS=Cucumis melo OX=3656 GN=LOC103489800 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.4e-167
Identity = 305/323 (94.43%), Postives = 306/323 (94.74%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAE KM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEXKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGLSKKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. ExPASy TrEMBL
Match: A0A0A0LCA6 (Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_3G624050 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.5e-166
Identity = 303/323 (93.81%), Postives = 306/323 (94.74%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLK SCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGLSKKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. ExPASy TrEMBL
Match: A0A6J1ESX1 (Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111437424 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.9e-162
Identity = 295/323 (91.33%), Postives = 301/323 (93.19%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG +GRGRRK T DENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. ExPASy TrEMBL
Match: A0A6J1ETJ9 (Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111437424 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.9e-162
Identity = 295/323 (91.33%), Postives = 301/323 (93.19%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG +GRGRRK T DENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. ExPASy TrEMBL
Match: A0A6J1K8G8 (Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111491607 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 5.1e-162
Identity = 294/330 (89.09%), Postives = 303/330 (91.82%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL+CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLSCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+++G +GRGRRK T DENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSIGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPR------------VVIMTWMMMILRKHEEI 300
           EEDEEEEAARKNRLGL+KKGGGDDDDEEGPR             +   W      +HEEI
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIESIGDDW------EHEEI 300

Query: 301 FTDDDETTAPDREEREDLAPEVPAPPEIKQ 319
           FTDDDETTAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 FTDDDETTAPDREEREDLAPEVPAPPEIKQ 324

[Close]

BLAST of PI0021021 vs. NCBI nr
Match: XP_016900419.1 (PREDICTED: LOW QUALITY PROTEIN: transcription initiation factor IIF subunit alpha [Cucumis melo])

HSP 1 Score: 599.0 bits (1543), Expect = 2.8e-167
Identity = 305/323 (94.43%), Postives = 306/323 (94.74%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAE KM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEXKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGLSKKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. NCBI nr
Match: XP_004152016.1 (transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >XP_011651639.1 transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >KGN58337.1 hypothetical protein Csa_017587 [Cucumis sativus])

HSP 1 Score: 595.5 bits (1534), Expect = 3.1e-166
Identity = 303/323 (93.81%), Postives = 306/323 (94.74%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLK SCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGLSKKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. NCBI nr
Match: XP_031738317.1 (transcription initiation factor IIF subunit alpha isoform X2 [Cucumis sativus])

HSP 1 Score: 595.5 bits (1534), Expect = 3.1e-166
Identity = 303/323 (93.81%), Postives = 306/323 (94.74%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLK SCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGLSKKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. NCBI nr
Match: XP_038888042.1 (transcription initiation factor IIF subunit alpha isoform X1 [Benincasa hispida])

HSP 1 Score: 591.3 bits (1523), Expect = 5.9e-165
Identity = 300/323 (92.88%), Postives = 304/323 (94.12%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDL+LKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLMLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYK+GLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKDGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKE VGTSGRGRRK TGDENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKEGVGTSGRGRRKPTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEA RKNRLGL+KKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAQRKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. NCBI nr
Match: XP_022931161.1 (transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 580.9 bits (1496), Expect = 8.0e-162
Identity = 295/323 (91.33%), Postives = 301/323 (93.19%), Query Frame = 0


Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG +GRGRRK T DENEGNVSDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KKGGGDDDDEEGPR          + K     HEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQ 319
           TAPDREEREDLAPEVPAPPEIKQ
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQ 323

[Close]

BLAST of PI0021021 vs. TAIR 10
Match: AT4G12610.1 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 223.8 bits (569), Expect = 2.3e-58
Identity = 129/183 (70.49%), Postives = 148/183 (80.87%), Query Frame = 0


Query: 146 LQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAF 205
           +Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKMKNR+KTADGY+RWMMKAA+NGPA F
Sbjct: 1   MQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGPALF 60

Query: 206 GEVEKFDDKETVGTS---GRGRRKTT-GDENEGNVSDRGEEDEEEEAARKNRLGLSKKGG 265
           GEV+  ++KE+ GTS   GRGR+K++ GDE EGNVSDRG+EDEEEEA+RK+RLGL++K  
Sbjct: 61  GEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRK-S 120

Query: 266 GDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDETTAPDREERED-LAPEVPAPPE 319
            DDDDEEGPR   +      + K     HEEIFTDDDE    D EERED LAPE+PAPPE
Sbjct: 121 NDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPE 180

[Close]

BLAST of PI0021021 vs. TAIR 10
Match: AT4G12610.2 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 223.8 bits (569), Expect = 2.3e-58
Identity = 129/183 (70.49%), Postives = 148/183 (80.87%), Query Frame = 0


Query: 146 LQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAF 205
           +Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKMKNR+KTADGY+RWMMKAA+NGPA F
Sbjct: 1   MQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGPALF 60

Query: 206 GEVEKFDDKETVGTS---GRGRRKTT-GDENEGNVSDRGEEDEEEEAARKNRLGLSKKGG 265
           GEV+  ++KE+ GTS   GRGR+K++ GDE EGNVSDRG+EDEEEEA+RK+RLGL++K  
Sbjct: 61  GEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRK-S 120

Query: 266 GDDDDEEGPRVVIMTWMMMILRK-----HEEIFTDDDETTAPDREERED-LAPEVPAPPE 319
            DDDDEEGPR   +      + K     HEEIFTDDDE    D EERED LAPE+PAPPE
Sbjct: 121 NDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPE 180

The following BLAST results are available for this feature:

BLAST of PI0021021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SU25	1.5e-123	71.65	Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=370...	[more]
Q54WV4	2.1e-08	27.94	General transcription factor IIF subunit 1 OS=Dictyostelium discoideum OX=44689 ...	[more]
O94416	3.8e-05	25.00	Transcription initiation factor IIF subunit alpha OS=Schizosaccharomyces pombe (...	[more]

BLAST of PI0021021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DWR2	1.4e-167	94.43	Transcription initiation factor IIF subunit alpha OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A0A0LCA6	1.5e-166	93.81	Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=...	[more]
A0A6J1ESX1	3.9e-162	91.33	Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1ETJ9	3.9e-162	91.33	Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1K8G8	5.1e-162	89.09	Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of PI0021021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016900419.1	2.8e-167	94.43	PREDICTED: LOW QUALITY PROTEIN: transcription initiation factor IIF subunit alph...	[more]
XP_004152016.1	3.1e-166	93.81	transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >...	[more]
XP_031738317.1	3.1e-166	93.81	transcription initiation factor IIF subunit alpha isoform X2 [Cucumis sativus]	[more]
XP_038888042.1	5.9e-165	92.88	transcription initiation factor IIF subunit alpha isoform X1 [Benincasa hispida]	[more]
XP_022931161.1	8.0e-162	91.33	transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita mos...	[more]

BLAST of PI0021021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12610.1	2.3e-58	70.49	transcription activators;DNA binding;RNA polymerase II transcription factors;cat...	[more]
AT4G12610.2	2.3e-58	70.49	transcription activators;DNA binding;RNA polymerase II transcription factors;cat...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008851	Transcription initiation factor IIF, alpha subunit	PFAM	PF05793	TFIIF_alpha	coord: 48..319 e-value: 4.1E-74 score: 250.1
IPR008851	Transcription initiation factor IIF, alpha subunit	PANTHER	PTHR13011	TFIIF-ALPHA	coord: 1..345
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..270
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 288..308
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 207..235
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 244..270
None	No IPR available	PANTHER	PTHR13011:SF3	BNAANNG16720D PROTEIN	coord: 1..345
IPR011039	Transcription Factor IIF, Rap30/Rap74, interaction	SUPERFAMILY	50916	Rap30/74 interaction domains	coord: 53..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0021021.1	PI0021021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032968	positive regulation of transcription elongation from RNA polymerase II promoter
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006413	translational initiation
cellular_component	GO:0005674	transcription factor TFIIF complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016251	RNA polymerase II general transcription initiation factor activity
molecular_function	GO:0001096	TFIIF-class transcription factor complex binding
molecular_function	GO:0003743	translation initiation factor activity