PI0020962 (gene) Melon (PI 482460) v1

Overview
NamePI0020962
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCysteine proteinase inhibitor
Locationchr10: 17272451 .. 17276512 (-)
RNA-Seq ExpressionPI0020962
SyntenyPI0020962
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAAATTGATTCTTTGCCCAAGAAATAAATAAAATGCTTAAACAAAACGCATCGCTTCGTGGCGCAGTTTCCTTATTGGCACGAATCCCATTTTACGATCGTGGCGCCAAGAGAGAGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGACTCTCATTCATATCTAACCGCGAATAAGTTTTTTCATAACATCGAAAGAGAAATGAAAGGAACTGTGGAAATCTCTCCTCTGTGATGAAAAAGGATTCTTCCGTTTCGTTTTCTCCATCATCATCTACTTTGTTGTTGTTGTTGTTGCTGTTTTTCTCTTGGATCATCGTTCTTGCAGGTAACGCATCAGCTCACTTTTGCGCTGAGCAGGACGATCCACTCCTTATGGCGTCCACACTTGGAGGCGTTCACGATTCTCAGGGAGCTTCCAACTCCACCGATGTTGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGGTAAACAGCTTCTCTCTCGATTTTTGTTTAAATGAATTTGTTTCTTACTCGGTGTTTTTTATCCTGTCGCGTTTAGTTGATTCTCGCTGATTGCGTCTGTGCTTTTTGTTGTCGATCTTGTTCTTGTTGTGTGTTGTGCTGTGGATTTCTGTGTTTCAGGCAGAATTTTGTTTGACTTGTTCTGCATTTTGATGCTGTCCGTTTCTGTCGGTGTCGTTAAGTAGTATGAGGATGTGTTGAACTAAAACTGCACGATTGTCTTCGTCTTTCGTTTGTTTGATAGATTGAATCGGTTGACTGTGGTGTGATTCTTTTTTCTTTAAGGAACTTGGCTGTCTATGGTTTTTAGGTTTGGATAGTGGATATGCGTAAATGTGTCTTTAGATTGACCATAGAATTTGAATTCGCGTCTAAAAGCTTGAAAAACTGAACCAGTCTTTCTGGCTATAAATAGTTAGGATTAACATTCAATTTTTATTATGAAAATCTTGCAATCTTCCATAAGATGATAACTGGAAACATGTTGGCGTTTTAATCTTTTCAAGGCTGATGGAGACCAGGTTAGCGAATGGTCCCTATGACGATGTGATTTCTGTTTATCTTCTGATCACTTCTAGTTACTTAAACAAGCACGTTTCCATGTCTTTAGAAGAGACGTTTGTTTCTCCGTTTCTCACAAGTGTTTTATGTTAATTCTATTTCAGTCTAAACACTCTTTCTATGAGATCCCAACTAATTGTATATTGATTTTATTTTAGTATAAGAACTCCTTTTACTATCAAATCCAATGTATATGTATACCAACTTCATTTCAGTTTAAAAGAGTTCTTTTTCTCTCATTTCTGAGTATTTGTATTGAAGTTTTATTTCAGCTTTTAAGTGGAAGTTCTACTTTGATTTCACGGGCTCTTTTTCCAGAACTAAGGAAACTTTCCTTTGAAAAATATCCACGATAAATTCGTTGGAAATTTACAAATTGGATACACATAAGCATAGTGTTGTAGTTGTTTGCAACTAAAAATTCCTCCTGTTCCTATTTCCTCTTTTCATGCAGCACACATATACTTTTCACATGTTATATATGTGTATGATGTAATTATCTTCCCATTCCTTTGCAGAATTCACTTCTTGAGTTTGTGAGAGTCGTGAAGGCAAAAGAGCAGGTAGTAGCTGGTACACTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCACAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGGATAACATTCTTTAACTCGTGCAATGCAGTTTCAATTACAATTTAAAGTATAGCTTTGAATATACAACCAATGTCAAATGAAATGTGTTGGCTCATATTCCACCTGTTTTTTTGGAGGTTATTTTCAGTTGAAGTCATTCATAACTTCTTGCAGGCTGATAAAATCTGAAGTTGTGGCTTTGTTTCTTTTCTAATTTACTAAGGGTTGATATTCTTGCAAACTTGAATCGACAACTATTTAGCAAACATTAGTAGCTCTATCGTAGAGCATAAGCATTCATTAGTTCTTGTATGTTTGTTTATATTCTTACAGGTCATAAAGCTGTAAATTTATGTGAAAATCTTGTAACTGATTTTAGAGTCAAATGTTGTTTTCTTTAATATACTATTGACCATGGTTAGAAATTAGGTGATCATCCCCAGGGATGGCGAGAAGTGCCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCGTATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTAAGTGGATTATGGTTCTTTTATTTAGTATGTTTTACCTCTGTTTAAGTTAGTTATCACAAGACTGTGAAGTTGATCTATGATATCAGGATTTGTCTGTCTTTTTCTTTCTTGCGTAATTGAAACTTGTTCATTGATGATGAAATAAAAGGACTAAACTCACGTCATTGATAGGTTATAAAAGAGCTCTCCAACTAGAAATCAACAAAGAAAGACTTTAGTCATTAAAAGGCTGTTTACATTGACACCAATACAAATAAAACGTATAAAATGAAATCAATTCCACAAAGCTAAATTTTTCCATTCCTCTTGCTCCAAAGAGGCCAAAGAATTTTTGTTTGTAGCAAACCCCACCGTGGAAAGGGTGGCGTTCCACAAAGACAAAAGACAAAAGCGTGATTATCCTAAATGCTGTTTGGAAATGGAACAAGGTTGGAATGAGGCTTTCTTTTTAGAGAATGAAATAGCTCCAAGACTTGGCTTCCCGTAGAAAGATTTCTAATTTTTTTTTTTTTTTGCTTTTTTGCTTTTTTGCTTTTTTTGTTTTCTTAGAAACATGAAAGAAAACAAGGGCATATAAAAAACTAAATCCGCAAAAATCCTACAAAAGGAGAGGGGTTCCAACTAGATAAAACGTTACCAAAAACATCTTTATATTCTTTGTTCCTTTATATTTTAGACATGTATGATTGACCAGAAAGTAAAGGTTGTATTCATCCCACAAGGCATTTAGTTTGAACTTTTGTAGGATTCACTGCGTTTCCATCATTTGTTTTAAAGCTTTTATGTTAATATCATTTTGATTAGCTTAACTTCTAATCTAACACTTCTATCAAACTCAATATGACCAACATTTTAAACCCGAGGAGAAGGTAGGCATTCATTCATACCCCATGTTCATACTTTATGAAAACTTTCTTCTGGTGAAGACTCAACATCCTAAATCGCATACCTTTCATTCATTTTAATTATCTTATTTATCAATCTACATAATACTAATGGAGAGATTCATGATGGTGTGAGAACAGGTGATTGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCACTCCTAAGAGAGTTTGAATTGTTCAATATGATTAAATAAAATACAAGTTTATGTACGATAGAACCTTTAAAGCTGTATACTATGCGAACTTTAACCTTCCCCTTCTTAACTTCTTTCGAAGCTCAGACTCCTCAGGCTGATACATAAAGGACCATGATAAATGATGTAGCCACTTATTAATTTGGTTTTGGATCTGTGGTTCACTTTTCTTCTGCTTTCCTTAATCCTTGCTTGAATTGTTAAGTGAACCTAGAAGGGTTTCTTAGAGGTTTTTTTGTTTCTGATATAGTTTTAGAAGGTCCTTTAAGAATCTCACACTGATATGAGTTATGTGGATATAAACTTAATTTGGGATTGTAAATTAGTATCATGTTGTATGTGTATTAATTTTGAACCATAGAATGGCAAATGAGTTGGTGTGATTTAACGTCACTATGAAAATTGCATTTAGAAGTGTATAATTAAATTTATTAATTTTAAGTGAATAAAAGTGATACTTTTTCTTTGCGTGCACTAAA

mRNA sequence

AAAAGAAATTGATTCTTTGCCCAAGAAATAAATAAAATGCTTAAACAAAACGCATCGCTTCGTGGCGCAGTTTCCTTATTGGCACGAATCCCATTTTACGATCGTGGCGCCAAGAGAGAGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGACTCTCATTCATATCTAACCGCGAATAAGTTTTTTCATAACATCGAAAGAGAAATGAAAGGAACTGTGGAAATCTCTCCTCTGTGATGAAAAAGGATTCTTCCGTTTCGTTTTCTCCATCATCATCTACTTTGTTGTTGTTGTTGTTGCTGTTTTTCTCTTGGATCATCGTTCTTGCAGGTAACGCATCAGCTCACTTTTGCGCTGAGCAGGACGATCCACTCCTTATGGCGTCCACACTTGGAGGCGTTCACGATTCTCAGGGAGCTTCCAACTCCACCGATGTTGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGAATTCACTTCTTGAGTTTGTGAGAGTCGTGAAGGCAAAAGAGCAGGTAGTAGCTGGTACACTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCACAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGATCATCCCCAGGGATGGCGAGAAGTGCCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCGTATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTGATTGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCACTCCTAAGAGAGTTTGAATTGTTCAATATGATTAAATAAAATACAAGTTTATGTACGATAGAACCTTTAAAGCTGTATACTATGCGAACTTTAACCTTCCCCTTCTTAACTTCTTTCGAAGCTCAGACTCCTCAGGCTGATACATAAAGGACCATGATAAATGATGTAGCCACTTATTAATTTGGTTTTGGATCTGTGGTTCACTTTTCTTCTGCTTTCCTTAATCCTTGCTTGAATTGTTAAGTGAACCTAGAAGGGTTTCTTAGAGGTTTTTTTGTTTCTGATATAGTTTTAGAAGGTCCTTTAAGAATCTCACACTGATATGAGTTATGTGGATATAAACTTAATTTGGGATTGTAAATTAGTATCATGTTGTATGTGTATTAATTTTGAACCATAGAATGGCAAATGAGTTGGTGTGATTTAACGTCACTATGAAAATTGCATTTAGAAGTGTATAATTAAATTTATTAATTTTAAGTGAATAAAAGTGATACTTTTTCTTTGCGTGCACTAAA

Coding sequence (CDS)

ATGAAAAAGGATTCTTCCGTTTCGTTTTCTCCATCATCATCTACTTTGTTGTTGTTGTTGTTGCTGTTTTTCTCTTGGATCATCGTTCTTGCAGGTAACGCATCAGCTCACTTTTGCGCTGAGCAGGACGATCCACTCCTTATGGCGTCCACACTTGGAGGCGTTCACGATTCTCAGGGAGCTTCCAACTCCACCGATGTTGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGAATTCACTTCTTGAGTTTGTGAGAGTCGTGAAGGCAAAAGAGCAGGTAGTAGCTGGTACACTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCACAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGATCATCCCCAGGGATGGCGAGAAGTGCCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCGTATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTGATTGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCACTCCTAA

Protein sequence

MKKDSSVSFSPSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Homology
BLAST of PI0020962 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.4e-80
Identity = 153/238 (64.29%), Postives = 188/238 (78.99%), Query Frame = 0

Query: 11  PSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDEL 70
           P+SS+    L LF   ++ +A  A+A F        +    LGG HD+  A+NS + D L
Sbjct: 9   PASSSAAAPLPLFL--LLAVAAAAAALFLVGSASLAMAGHVLGGAHDAPSAANSVETDAL 68

Query: 71  ARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMN 130
           ARFAV+EHNK+EN+LLEFVRVV+AKEQVVAGTLHHLT+E +EAG+KK+YEAKVWVK W++
Sbjct: 69  ARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLD 128

Query: 131 FKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSL 190
           FKELQEF++ GD  T T +DLGAKKG H  GWR+VP HDP V+DAA HA+++IQQRSNSL
Sbjct: 129 FKELQEFRNTGDATTFTNADLGAKKGGHEPGWRDVPVHDPVVKDAADHAVKSIQQRSNSL 188

Query: 191 VPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDH 249
            PYELLEI+ AKAEV+ED AKFD+L+KLKRG+KEEKFK EVHKN EG F+LNQM Q+H
Sbjct: 189 FPYELLEIVRAKAEVVEDFAKFDILMKLKRGNKEEKFKAEVHKNLEGAFVLNQMQQEH 244

BLAST of PI0020962 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 6.8e-75
Identity = 147/233 (63.09%), Postives = 179/233 (76.82%), Query Frame = 0

Query: 18  LLLLLFFSWIIVLAGNASAH--FCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAV 77
           LL ++FFS  + ++   ++   FC E+       + +GGV D     NS +V+ LARFAV
Sbjct: 7   LLFIVFFSLSLFISSLIASDLGFCNEE------MALVGGVGDVPANQNSGEVESLARFAV 66

Query: 78  EEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQ 137
           +EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++EAG+KKLYEAKVWVK W+NFKELQ
Sbjct: 67  DEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQ 126

Query: 138 EFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYEL 197
           EFK A D P IT SDLG K+G+H  GWREVP  DP V+  A+ A++TIQQRSNSL PYEL
Sbjct: 127 EFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQRSNSLFPYEL 186

Query: 198 LEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDH 249
           LE++HAKAEV  +AAK+++LLKLKRG KEEKFKVEVHKN+EG   LN   Q H
Sbjct: 187 LEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLNHAEQHH 233

BLAST of PI0020962 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 4.3e-53
Identity = 111/226 (49.12%), Postives = 165/226 (73.01%), Query Frame = 0

Query: 18  LLLLLFFSWIIVLAGNASAHFCAEQD---DPLLMASTLGGVHDSQGASN-STDVDELARF 77
           + ++L    +++L+G      C+E+    +  ++   LGG  DS+   N   ++D++A F
Sbjct: 8   MCMMLICVSLVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIALF 67

Query: 78  AVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKE 137
           AV+EHN++EN++LE  RV+KA EQVVAG L+ LT+EV+EAG+KK+YEAKVWVK WMNFK+
Sbjct: 68  AVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQ 127

Query: 138 LQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPY 197
           LQEFK+   +P+ T SDLG K   +   WR V  ++P VQ+AA+HA++++QQ+SNSL PY
Sbjct: 128 LQEFKNI--IPSFTISDLGFKPDGNGFDWRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPY 187

Query: 198 ELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           +L++II A+A+V+E+  KF+LLLKL+RG+K EKF VEV K+  G +
Sbjct: 188 KLIDIILARAKVVEERVKFELLLKLERGNKLEKFMVEVMKDQTGKY 231

BLAST of PI0020962 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 9.6e-29
Identity = 61/95 (64.21%), Postives = 76/95 (80.00%), Query Frame = 0

Query: 50  STLGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVE 109
           + LGG  D  G  NS ++D LARFAVEEHNKK+N+LLEF RVV A++QVV+GTL+ +T+E
Sbjct: 2   AALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLE 61

Query: 110 VVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVP 145
             + G+KK+YEAKVW K W+NFKELQEFKH GD P
Sbjct: 62  AKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGDAP 96

BLAST of PI0020962 vs. ExPASy Swiss-Prot
Match: Q41906 (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 2.1e-28
Identity = 60/117 (51.28%), Postives = 83/117 (70.94%), Query Frame = 0

Query: 26  WII--VLAGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAVEEHNKKEN 85
           WI+  +L G      C  ++        LGGVHD +G  NS +++ LARFA++EHNK++N
Sbjct: 7   WIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQN 66

Query: 86  SLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHA 141
            +LEF ++VKA+EQVVAGT++HLT+E  E  + K +EAKVWVK WMNFK+LQEFK +
Sbjct: 67  KILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

BLAST of PI0020962 vs. ExPASy TrEMBL
Match: A0A5D3CW86 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002960 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 8.4e-129
Identity = 241/250 (96.40%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTL-LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M   S++SFSP SSTL LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVP+I+PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVQDHS 250
           LLNQMVQDHS
Sbjct: 241 LLNQMVQDHS 250

BLAST of PI0020962 vs. ExPASy TrEMBL
Match: A0A1S3BEK2 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 8.4e-129
Identity = 241/250 (96.40%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTL-LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M   S++SFSP SSTL LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVP+I+PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVQDHS 250
           LLNQMVQDHS
Sbjct: 241 LLNQMVQDHS 250

BLAST of PI0020962 vs. ExPASy TrEMBL
Match: A0A5A7SXY4 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001180 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.5e-128
Identity = 240/250 (96.00%), Postives = 244/250 (97.60%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTL-LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M   S++SFSP SSTL LLLLLLFFSWIIVL GNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVP+I+PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVQDHS 250
           LLNQMVQDHS
Sbjct: 241 LLNQMVQDHS 250

BLAST of PI0020962 vs. ExPASy TrEMBL
Match: A0A0A0KV53 (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.3e-126
Identity = 237/249 (95.18%), Postives = 241/249 (96.79%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQG 60
           M KD+S+SFSP SS LLLLLLLFFS IIVLAGNASAHFCA+QDDPLLMASTLG V DSQG
Sbjct: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60

Query: 61  ASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE
Sbjct: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180
           AKVWVKSWMNFKELQEFKHAGDVP+ITPSDLGAKKGDHPQGWREV PHDPHVQDAAQHAL
Sbjct: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240
           RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL
Sbjct: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240

Query: 241 LNQMVQDHS 250
           LNQMVQDHS
Sbjct: 241 LNQMVQDHS 249

BLAST of PI0020962 vs. ExPASy TrEMBL
Match: A0A6J1H1C9 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.9e-118
Identity = 225/249 (90.36%), Postives = 234/249 (93.98%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQG 60
           MK DSS+ F  SSST LLLLLL  SW IVLAGNASAHFCA+QDDPLLMASTLGGVHDSQG
Sbjct: 1   MKNDSSIWF--SSSTFLLLLLL--SW-IVLAGNASAHFCAQQDDPLLMASTLGGVHDSQG 60

Query: 61  ASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNS D DELARFAV+EHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLYE
Sbjct: 61  ASNSADFDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180
           AK+WVKSWMNFKELQEFKHAGD P+ +PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL
Sbjct: 121 AKIWVKSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240
           RTIQQRSNSL+PYELLEI+HAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL
Sbjct: 181 RTIQQRSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240

Query: 241 LNQMVQDHS 250
           LNQMV D S
Sbjct: 241 LNQMVPDQS 244

BLAST of PI0020962 vs. NCBI nr
Match: XP_008445944.1 (PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 469.5 bits (1207), Expect = 1.7e-128
Identity = 241/250 (96.40%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTL-LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M   S++SFSP SSTL LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVP+I+PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVQDHS 250
           LLNQMVQDHS
Sbjct: 241 LLNQMVQDHS 250

BLAST of PI0020962 vs. NCBI nr
Match: KAA0034127.1 (cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 468.0 bits (1203), Expect = 5.1e-128
Identity = 240/250 (96.00%), Postives = 244/250 (97.60%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTL-LLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M   S++SFSP SSTL LLLLLLFFSWIIVL GNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVP+I+PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVQDHS 250
           LLNQMVQDHS
Sbjct: 241 LLNQMVQDHS 250

BLAST of PI0020962 vs. NCBI nr
Match: XP_004147084.1 (cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical protein Csa_008462 [Cucumis sativus])

HSP 1 Score: 461.5 bits (1186), Expect = 4.7e-126
Identity = 237/249 (95.18%), Postives = 241/249 (96.79%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQG 60
           M KD+S+SFSP SS LLLLLLLFFS IIVLAGNASAHFCA+QDDPLLMASTLG V DSQG
Sbjct: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60

Query: 61  ASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE
Sbjct: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180
           AKVWVKSWMNFKELQEFKHAGDVP+ITPSDLGAKKGDHPQGWREV PHDPHVQDAAQHAL
Sbjct: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240
           RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL
Sbjct: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240

Query: 241 LNQMVQDHS 250
           LNQMVQDHS
Sbjct: 241 LNQMVQDHS 249

BLAST of PI0020962 vs. NCBI nr
Match: XP_038891963.1 (cysteine proteinase inhibitor 12 [Benincasa hispida])

HSP 1 Score: 443.4 bits (1139), Expect = 1.3e-120
Identity = 226/249 (90.76%), Postives = 239/249 (95.98%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQG 60
           M K+SS+S    SS++ LLLLL FSWIIVLAGNASA FCA+QDDPLLMASTLGGV DSQG
Sbjct: 1   MNKNSSISL---SSSIALLLLLLFSWIIVLAGNASAQFCAQQDDPLLMASTLGGVRDSQG 60

Query: 61  ASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNSTDVDELARFAV+EHNKKENS+LEFVRVVKAKEQVVAGTLHHLT+EVV+AGKKKLYE
Sbjct: 61  ASNSTDVDELARFAVDEHNKKENSILEFVRVVKAKEQVVAGTLHHLTLEVVDAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180
           AKVWVKSWMNFKELQEFKHAGDVP+IT SDLGAKKGDHPQGWREVPPHDPHVQDAAQHA+
Sbjct: 121 AKVWVKSWMNFKELQEFKHAGDVPSITSSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAV 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240
           +TIQQRSNSL+PYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL
Sbjct: 181 QTIQQRSNSLLPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240

Query: 241 LNQMVQDHS 250
           LNQMVQDHS
Sbjct: 241 LNQMVQDHS 246

BLAST of PI0020962 vs. NCBI nr
Match: XP_022957234.1 (cysteine proteinase inhibitor 12-like [Cucurbita moschata])

HSP 1 Score: 434.1 bits (1115), Expect = 8.1e-118
Identity = 225/249 (90.36%), Postives = 234/249 (93.98%), Query Frame = 0

Query: 1   MKKDSSVSFSPSSSTLLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQG 60
           MK DSS+ F  SSST LLLLLL  SW IVLAGNASAHFCA+QDDPLLMASTLGGVHDSQG
Sbjct: 1   MKNDSSIWF--SSSTFLLLLLL--SW-IVLAGNASAHFCAQQDDPLLMASTLGGVHDSQG 60

Query: 61  ASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNS D DELARFAV+EHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLYE
Sbjct: 61  ASNSADFDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180
           AK+WVKSWMNFKELQEFKHAGD P+ +PSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL
Sbjct: 121 AKIWVKSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240
           RTIQQRSNSL+PYELLEI+HAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL
Sbjct: 181 RTIQQRSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFL 240

Query: 241 LNQMVQDHS 250
           LNQMV D S
Sbjct: 241 LNQMVPDQS 244

BLAST of PI0020962 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 282.0 bits (720), Expect = 4.8e-76
Identity = 147/233 (63.09%), Postives = 179/233 (76.82%), Query Frame = 0

Query: 18  LLLLLFFSWIIVLAGNASAH--FCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAV 77
           LL ++FFS  + ++   ++   FC E+       + +GGV D     NS +V+ LARFAV
Sbjct: 7   LLFIVFFSLSLFISSLIASDLGFCNEE------MALVGGVGDVPANQNSGEVESLARFAV 66

Query: 78  EEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQ 137
           +EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++EAG+KKLYEAKVWVK W+NFKELQ
Sbjct: 67  DEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQ 126

Query: 138 EFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYEL 197
           EFK A D P IT SDLG K+G+H  GWREVP  DP V+  A+ A++TIQQRSNSL PYEL
Sbjct: 127 EFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQRSNSLFPYEL 186

Query: 198 LEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDH 249
           LE++HAKAEV  +AAK+++LLKLKRG KEEKFKVEVHKN+EG   LN   Q H
Sbjct: 187 LEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLNHAEQHH 233

BLAST of PI0020962 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 278.9 bits (712), Expect = 4.1e-75
Identity = 139/197 (70.56%), Postives = 162/197 (82.23%), Query Frame = 0

Query: 52  LGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVV 111
           +GGV D     NS +V+ LARFAV+EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL 63

Query: 112 EAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPH 171
           EAG+KKLYEAKVWVK W+NFKELQEFK A D P IT SDLG K+G+H  GWREVP  DP 
Sbjct: 64  EAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPE 123

Query: 172 VQDAAQHALRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEV 231
           V+  A+ A++TIQQRSNSL PYELLE++HAKAEV  +AAK+++LLKLKRG KEEKFKVEV
Sbjct: 124 VKHVAEQAVKTIQQRSNSLFPYELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEV 183

Query: 232 HKNNEGNFLLNQMVQDH 249
           HKN+EG   LN   Q H
Sbjct: 184 HKNHEGALHLNHAEQHH 200

BLAST of PI0020962 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 209.5 bits (532), Expect = 3.0e-54
Identity = 111/226 (49.12%), Postives = 165/226 (73.01%), Query Frame = 0

Query: 18  LLLLLFFSWIIVLAGNASAHFCAEQD---DPLLMASTLGGVHDSQGASN-STDVDELARF 77
           + ++L    +++L+G      C+E+    +  ++   LGG  DS+   N   ++D++A F
Sbjct: 8   MCMMLICVSLVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIALF 67

Query: 78  AVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKE 137
           AV+EHN++EN++LE  RV+KA EQVVAG L+ LT+EV+EAG+KK+YEAKVWVK WMNFK+
Sbjct: 68  AVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQ 127

Query: 138 LQEFKHAGDVPTITPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPY 197
           LQEFK+   +P+ T SDLG K   +   WR V  ++P VQ+AA+HA++++QQ+SNSL PY
Sbjct: 128 LQEFKNI--IPSFTISDLGFKPDGNGFDWRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPY 187

Query: 198 ELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240
           +L++II A+A+V+E+  KF+LLLKL+RG+K EKF VEV K+  G +
Sbjct: 188 KLIDIILARAKVVEERVKFELLLKLERGNKLEKFMVEVMKDQTGKY 231

BLAST of PI0020962 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-29
Identity = 60/117 (51.28%), Postives = 83/117 (70.94%), Query Frame = 0

Query: 26  WII--VLAGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAVEEHNKKEN 85
           WI+  +L G      C  ++        LGGVHD +G  NS +++ LARFA++EHNK++N
Sbjct: 7   WIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQN 66

Query: 86  SLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHA 141
            +LEF ++VKA+EQVVAGT++HLT+E  E  + K +EAKVWVK WMNFK+LQEFK +
Sbjct: 67  KILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

BLAST of PI0020962 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 102.4 bits (254), Expect = 5.2e-22
Identity = 51/92 (55.43%), Postives = 64/92 (69.57%), Query Frame = 0

Query: 52  LGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVV 111
           +GGV D    +N   V+ LARFAV+EHNK EN  LE+ R++ AK QVVAGT+HHLTVEV 
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 112 EAGKKKLYEAKVWVKSWMNFKELQEFKHAGDV 144
           +    K+YEAKV  K+W N K+L+ F H  DV
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESFNHLHDV 101

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0JNR21.4e-8064.29Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0... [more]
Q8H0X66.8e-7563.09Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=... [more]
Q8LC764.3e-5349.12Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=... [more]
Q064459.6e-2964.21Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1[more]
Q419062.1e-2851.28Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A5D3CW868.4e-12996.40Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BEK28.4e-12996.40Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1[more]
A0A5A7SXY42.5e-12896.00Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A0A0KV532.3e-12695.18Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV... [more]
A0A6J1H1C93.9e-11890.36Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3... [more]
Match NameE-valueIdentityDescription
XP_008445944.11.7e-12896.40PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine ... [more]
KAA0034127.15.1e-12896.00cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa][more]
XP_004147084.14.7e-12695.18cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical prot... [more]
XP_038891963.11.3e-12090.76cysteine proteinase inhibitor 12 [Benincasa hispida][more]
XP_022957234.18.1e-11890.36cysteine proteinase inhibitor 12-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G12490.24.8e-7663.09cystatin B [more]
AT3G12490.14.1e-7570.56cystatin B [more]
AT5G05110.13.0e-5449.12Cystatin/monellin family protein [more]
AT2G40880.11.5e-2951.28cystatin A [more]
AT5G12140.15.2e-2255.43cystatin-1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 50..140
e-value: 3.8E-33
score: 126.1
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 64..139
e-value: 1.4E-20
score: 73.4
IPR000010Cystatin domainCDDcd00042CYcoord: 53..139
e-value: 6.51707E-23
score: 87.9148
NoneNo IPR availableGENE3D3.10.450.10coord: 48..147
e-value: 7.2E-42
score: 144.0
NoneNo IPR availableGENE3D3.10.450.10coord: 149..243
e-value: 1.5E-10
score: 43.2
NoneNo IPR availablePANTHERPTHR11413:SF103CYSTEINE PROTEINASE INHIBITOR 6coord: 51..245
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 51..143
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 154..241
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 51..245
IPR018073Proteinase inhibitor I25, cystatin, conserved sitePROSITEPS00287CYSTATINcoord: 96..109

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0020962.1PI0020962.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity