PI0020859 (gene) Melon (PI 482460) v1

Overview


Name	PI0020859
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Non-lysosomal glucosylceramidase
Location	chr10: 3138403 .. 3141297 (-)
RNA-Seq Expression	PI0020859
Synteny	PI0020859

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGAGGGACAATCTGGACAGGTATATCTAACCTACCATTTTAAGATTGTTATTTATGATTTTGATTAAAATTGTATCATATTTTTTAAAATGTTTGTATAGATGGGTCGCTTCCAATACAGAGTTTAGTAAGCATAGGGGAAAGAGGATTTTGCCTTGATCAATGCAGATCTGGTCTGAAGAGTGTAATTTATGAGTCCCATGAAAATGACACTGCTAATGATATTCTTGGGAGGATGACTTCAAGACTTGATGAGTTACGTGATTCAGTCGCGTCGAATTCTGCATTTGGAGTAAATCTGCTTCAAAAGGGAGAGGAGAATGTTGGGCAGTTCCTTTACCTTGAAGGGGTTGAATACACAATGTGGAATACCTATGACGTTCATTTTTACTCCTCTTTTGCAATAATAATGCTATTTCCAAAACTCGAACTCAGCATTCAACGAGATTTTGCAGCAGCAGTGATGATGCATGATCCCAGCAAGATGCAACTTCTTGATAATGGGAAATGGGAAGCAAGAAATGTTCTTGGAGCTGTTCCCCATGATATCGGAGTTAATGATCCATGGTTTGAAGTGAATGGATATAACCTATATAATACGGATAGGTGGAAGGACTTGAATCCAAAATTTGTCCTCCAAATTTATAGGGATGTTGTTGCCACGGGGATATGAAATTTGCAAAGGCTGTCTGGCCCCCTGTCTATCTTGCCATAGCTTATATGGACCAGTTCGATCGAGATGGTGATGGGATGATTGAGAATGATGGGTTCCCCGATCAGACTTATGATACATGGTCCGTCACTGGTGTTAGTGCATACAGTGGTGGCTTATGGGTGGCAGCGTTGCAGGCTGCTTCAGCCCTGGCTCGTGTAGCCGATGAAAAGGATGCTGAACACTACTTTTGGTTCAAATTTCAGAAGGCAAAACGTGCCTATGAGAAATTGTGGAATGGTTCTTACTTCAACTATGACAGTAGTGGCGGGAGTTCAAGTTCATCCATTCAAGCTGATCAGTTGGCTGGACAATGGTATATATGTAATAATCTTGAAGTTTATTAATAATAATAAAAAGTTTAGCATAATGCTATACAACTCAAAGGAATTATCTCTTTAATATACTTCTTTTTCTAATTCATCGGCAGTTAGGTTTTTCCTTAGTTTGGACTAGAATCTAGAATTAAATATTAGATGATATAATATTAAATCTACCTTCACCCATTGGTTTAAGCTTTTTGGGTTAGACGGTGATTTAAAATAGTATCAAAGATGGTCTAGGGGAGGTCCTATGTTTAAACCTGTGTAATATTAGTTCCTCCCCATTTAATATTGATTTCCACTTGTTGAGTCCTCAAAAGAATGATGCACAAAACTAACTCAAATTAATATTGTTAAACTGTAGAGTAGGAACTGCCTTTCAACTTTAATCTCAATCGGAAATCCTCCATCTTTCCTCCCATTTTCAACATTGTATGAATGTGAGACTATATAATTTATGGACTTCTAATAGCAACATAATATTTATCTGCAAGGTATGCAAGAGCTTCAGGTCTTCTTCCCATTGTTGATGAAGACAAGGCCAAAAGTGCACTGGCGAAGGTATATAATTACAATGTCATGAAGGTGAAGGATGGGAAGCGAGGTGCAGTAAATGGGATGCTTCCAGATGGAACGGTAGACTTTTCGTCAATGCAGTCAAGAGAAATATGGTCAGGAGTGACATATGCGGTTGCTGCTTCAATGATCCATGAAAATATGACTGACATGGCATTTCGAACAGCAGAAGGCATCCATGAAACTGCATGGTCAGAAGATGGCCTGGGGTAAGTCAGATCATACATATATATACACTAACATACATACATATAGATACATATACATATACATATACATACATATACATATACATATATACATATATACATACTTATTTCATTTTGTTTCTTTTTGCACTTCCACCATGTAGACTGTTTTCAATTAAATCTCAGATGATTTCCTATGGCAAGAAGCTTGACGGTCTGGATGTCACTTACATACTGAAGATTTCTTTTGCATAACTTATAGAATAAGCCGCCTATTAACCAAGTTGTTTCTTAGTTTCACGAAATGGTTTCTATTTTTGTTTTGAAAAGGATGCCGAGCTTTTCATTAAGAAAATGAGAAGAACCGACTAATGCTATGAAATGCGAAGTAAACAAAATATAATCAAATAAGTCATCAAAAGTACAAAATAAGGGAAATCTACTAAAGAAAAACAATAAAACTAACTATCCCAGCTCCTGTTTATACCCTAGATGGAATAGCCTTTAAAGCTTTCAGATAATGAACACCATGATGAACATTCAAATGGGCTGAATCAAAACACTCCAAACATGAACGTGACGATTCCTAAAATATATGTTGATTTCTTCCATTCCAAAGCTCATGTAAAAGACCAATTAACTGTATCAATCTGCAATAAATAAGTTTTTACACTCAATAGAGGGCTGCACAATATCTAAAGAACATACAACATGGTTTTTATTTGAACCATCCATATTCTCTTTAGCGCCTAGATATGTAAGTATACGTGTCTTTCACTCAGAAATTGACTCATATTTATAAATTTTTTTGTTGTGATCAGATACAACTTTCAGACACCAGAAGCTTGGACAACCACAGATCGGTACAGATCATTGTGTTACATGCGACCTCTTGCAATTTGGGCGATGCAATGGGCATTTTCAGAAAAAGTATCTGTCATTGAGGAGTTGAAAGAACTTGATAGCGATGCCATATTAAGACACCATGCTAAGTTTTCAAAAGTTGCCCGCCTTTTGAAGTTGCCTGAAGATGGGACATCTTCAAGTGTACTACAAACCGTCTATGATTACACCCTTAAGAGGTTTTTCTAG

mRNA sequence

ATGCAGGAGGGACAATCTGGACAGAGTTTAGTAAGCATAGGGGAAAGAGGATTTTGCCTTGATCAATGCAGATCTGGTCTGAAGAGTGTAATTTATGAGTCCCATGAAAATGACACTGCTAATGATATTCTTGGGAGGATGACTTCAAGACTTGATGAGTTACGTGATTCAGTCGCGTCGAATTCTGCATTTGGAGTAAATCTGCTTCAAAAGGGAGAGGAGAATGTTGGGCAGTTCCTTTACCTTGAAGGGGTTGAATACACAATGTGGAATACCTATGACGTTCATTTTTACTCCTCTTTTGCAATAATAATGCTATTTCCAAAACTCGAACTCAGCATTCAACGAGATTTTGCAGCAGCAGTGATGATGCATGATCCCAGCAAGATGCAACTTCTTGATAATGGGAAATGGGAAGCAAGAAATGTTCTTGGAGCTGTTCCCCATGATATCGGAGTTAATGATCCATGGTTTGAAGTGAATGGATATAACCTATATAATACGGATAGGTGGAAGGACTTGAATCCAAAATTTGTCCTCCAAATTTATAGGGATGTTGCTGTCTGGCCCCCTGTCTATCTTGCCATAGCTTATATGGACCAGTTCGATCGAGATGGTGATGGGATGATTGAGAATGATGGGTTCCCCGATCAGACTTATGATACATGGTCCGTCACTGGTGTTAGTGCATACAGTGGTGGCTTATGGGTGGCAGCGTTGCAGGCTGCTTCAGCCCTGGCTCGTGTAGCCGATGAAAAGGATGCTGAACACTACTTTTGGTTCAAATTTCAGAAGGCAAAACGTGCCTATGAGAAATTGTGGAATGGTTCTTACTTCAACTATGACAGTAGTGGCGGGAGTTCAAGTTCATCCATTCAAGCTGATCAGTTGGCTGGACAATGGTATGCAAGAGCTTCAGGTCTTCTTCCCATTGTTGATGAAGACAAGGCCAAAAGTGCACTGGCGAAGGTATATAATTACAATGTCATGAAGGTGAAGGATGGGAAGCGAGGTGCAGTAAATGGGATGCTTCCAGATGGAACGGTAGACTTTTCGTCAATGCAGTCAAGAGAAATATGGTCAGGAGTGACATATGCGGTTGCTGCTTCAATGATCCATGAAAATATGACTGACATGGCATTTCGAACAGCAGAAGGCATCCATGAAACTGCATGGTCAGAAGATGGCCTGGGATACAACTTTCAGACACCAGAAGCTTGGACAACCACAGATCGGTACAGATCATTGTGTTACATGCGACCTCTTGCAATTTGGGCGATGCAATGGGCATTTTCAGAAAAAGTATCTGTCATTGAGGAGTTGAAAGAACTTGATAGCGATGCCATATTAAGACACCATGCTAAGTTTTCAAAAGTTGCCCGCCTTTTGAAGTTGCCTGAAGATGGGACATCTTCAAGTGTACTACAAACCGTCTATGATTACACCCTTAAGAGGTTTTTCTAG

Coding sequence (CDS)

ATGCAGGAGGGACAATCTGGACAGAGTTTAGTAAGCATAGGGGAAAGAGGATTTTGCCTTGATCAATGCAGATCTGGTCTGAAGAGTGTAATTTATGAGTCCCATGAAAATGACACTGCTAATGATATTCTTGGGAGGATGACTTCAAGACTTGATGAGTTACGTGATTCAGTCGCGTCGAATTCTGCATTTGGAGTAAATCTGCTTCAAAAGGGAGAGGAGAATGTTGGGCAGTTCCTTTACCTTGAAGGGGTTGAATACACAATGTGGAATACCTATGACGTTCATTTTTACTCCTCTTTTGCAATAATAATGCTATTTCCAAAACTCGAACTCAGCATTCAACGAGATTTTGCAGCAGCAGTGATGATGCATGATCCCAGCAAGATGCAACTTCTTGATAATGGGAAATGGGAAGCAAGAAATGTTCTTGGAGCTGTTCCCCATGATATCGGAGTTAATGATCCATGGTTTGAAGTGAATGGATATAACCTATATAATACGGATAGGTGGAAGGACTTGAATCCAAAATTTGTCCTCCAAATTTATAGGGATGTTGCTGTCTGGCCCCCTGTCTATCTTGCCATAGCTTATATGGACCAGTTCGATCGAGATGGTGATGGGATGATTGAGAATGATGGGTTCCCCGATCAGACTTATGATACATGGTCCGTCACTGGTGTTAGTGCATACAGTGGTGGCTTATGGGTGGCAGCGTTGCAGGCTGCTTCAGCCCTGGCTCGTGTAGCCGATGAAAAGGATGCTGAACACTACTTTTGGTTCAAATTTCAGAAGGCAAAACGTGCCTATGAGAAATTGTGGAATGGTTCTTACTTCAACTATGACAGTAGTGGCGGGAGTTCAAGTTCATCCATTCAAGCTGATCAGTTGGCTGGACAATGGTATGCAAGAGCTTCAGGTCTTCTTCCCATTGTTGATGAAGACAAGGCCAAAAGTGCACTGGCGAAGGTATATAATTACAATGTCATGAAGGTGAAGGATGGGAAGCGAGGTGCAGTAAATGGGATGCTTCCAGATGGAACGGTAGACTTTTCGTCAATGCAGTCAAGAGAAATATGGTCAGGAGTGACATATGCGGTTGCTGCTTCAATGATCCATGAAAATATGACTGACATGGCATTTCGAACAGCAGAAGGCATCCATGAAACTGCATGGTCAGAAGATGGCCTGGGATACAACTTTCAGACACCAGAAGCTTGGACAACCACAGATCGGTACAGATCATTGTGTTACATGCGACCTCTTGCAATTTGGGCGATGCAATGGGCATTTTCAGAAAAAGTATCTGTCATTGAGGAGTTGAAAGAACTTGATAGCGATGCCATATTAAGACACCATGCTAAGTTTTCAAAAGTTGCCCGCCTTTTGAAGTTGCCTGAAGATGGGACATCTTCAAGTGTACTACAAACCGTCTATGATTACACCCTTAAGAGGTTTTTCTAG

Protein sequence

MQEGQSGQSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSREIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQTVYDYTLKRFF

Homology

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BLAST of PI0020859 vs. ExPASy Swiss-Prot
Match: Q69ZF3 (Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2)

HSP 1 Score: 325.1 bits (832), Expect = 1.4e-87
Identity = 180/396 (45.45%), Postives = 234/396 (59.09%), Query Frame = 0


Query: 56  DSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQ 115
           DS+       +  L+   ++ G+F YLEG EY M+NTYDVHFY+SFA++ML+PKLELS+Q
Sbjct: 491 DSLPEGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQ 550

Query: 116 RDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKD 175
            D A A +  D ++ + L +G          +PHDIG  D  PW  VN Y +++T  WKD
Sbjct: 551 YDMALATLKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKD 610

Query: 176 LNPKFVLQIYRDV----------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTW 235
           LN KFVLQIYRD            +WP     +    +FD+D DG+IEN G+ DQTYD W
Sbjct: 611 LNLKFVLQIYRDYYLTGDQGFLEDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAW 670

Query: 236 SVTGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGSYFNYD 295
             TG SAY GGLW+AA+     +A +   +D +  F     + + AYE+ LWNG Y+NYD
Sbjct: 671 VTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQERFASILCRGREAYERLLWNGRYYNYD 730

Query: 296 SSGGSSSSSIQADQLAGQWYARASGL----LPIVDEDKAKSALAKVYNYNVMKVKDGKRG 355
           SS    S SI +DQ AGQW+ RA GL      +        AL  ++  NV     G  G
Sbjct: 731 SSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALQTIFELNVQAFAGGAMG 790

Query: 356 AVNGMLPDGTVDFSSMQSREIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLG 415
           AVNGM P G  D SS+QS E+W GV Y +AA+MI E +T   FRTAEG + T W  + LG
Sbjct: 791 AVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLG 850

Query: 416 YNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEK 435
             FQTPEA+     +RSL YMRPL+IWAMQ A  ++
Sbjct: 851 LAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLALQQQ 884

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BLAST of PI0020859 vs. ExPASy Swiss-Prot
Match: Q9HCG7 (Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2)

HSP 1 Score: 323.9 bits (829), Expect = 3.0e-87
Identity = 179/401 (44.64%), Postives = 236/401 (58.85%), Query Frame = 0


Query: 51  LDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKL 110
           L+ L DS+       +  L+    + G+F YLEG EY M+NTYDVHFY+SFA+IML+PKL
Sbjct: 495 LEVLEDSLPEELGRNMCHLRPTLRDYGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKL 554

Query: 111 ELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNT 170
           ELS+Q D A A +  D ++ + L +G          +PHDIG  D  PW  VN Y +++T
Sbjct: 555 ELSLQYDMALATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDT 614

Query: 171 DRWKDLNPKFVLQIYRDV----------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQ 230
             WKDLN KFVLQ+YRD            +WP     +    +FD+D DG+IEN G+ DQ
Sbjct: 615 ADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQ 674

Query: 231 TYDTWSVTGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGS 290
           TYD W  TG SAY GGLW+AA+     +A +   +D +  F     + + AYE+ LWNG 
Sbjct: 675 TYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGR 734

Query: 291 YFNYDSSGGSSSSSIQADQLAGQWYARASGL----LPIVDEDKAKSALAKVYNYNVMKVK 350
           Y+NYDSS    S S+ +DQ AGQW+ +A GL      +        AL  ++  NV    
Sbjct: 735 YYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFA 794

Query: 351 DGKRGAVNGMLPDGTVDFSSMQSREIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWS 410
            G  GAVNGM P G  D SS+QS E+W GV Y +AA+MI E +T   F+TAEG + T W 
Sbjct: 795 GGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVW- 854

Query: 411 EDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEK 435
            + LG  FQTPEA+     +RSL YMRPL+IWAMQ A  ++
Sbjct: 855 -ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQ 893

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BLAST of PI0020859 vs. ExPASy Swiss-Prot
Match: Q5M868 (Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2)

HSP 1 Score: 320.5 bits (820), Expect = 3.4e-86
Identity = 175/378 (46.30%), Postives = 227/378 (60.05%), Query Frame = 0


Query: 74  ENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLL 133
           ++ G+F YLEG EY M+NTYDVHFY+SFA++ML+PKLELS+Q D A A    D ++ + L
Sbjct: 509 QDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYL 568

Query: 134 DNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV----- 193
            +G          +PHDIG  D  PW  VN Y +++T  WKDLN KFVLQ+YRD      
Sbjct: 569 MSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGD 628

Query: 194 -----AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQ 253
                 +WP     +    +FD+D DG+IEN G+ DQTYD W  TG SAY GGLW+AA+ 
Sbjct: 629 QGFLKDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVA 688

Query: 254 AASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGSYFNYDSSGGSSSSSIQADQLAGQ 313
               +A +   +D +  F     + + AYE+ LWNG Y+NYDSS    S S+ +DQ AGQ
Sbjct: 689 VMVQMAVLCGAQDVQDKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCAGQ 748

Query: 314 WYARASGL----LPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQS 373
           W+ RA GL      +        AL  ++  NV     G  GAVNGM P G  D SS+QS
Sbjct: 749 WFLRACGLGEGDTEVFPTLHVVRALKTIFELNVQAFAGGAMGAVNGMQPHGVPDRSSVQS 808

Query: 374 REIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSL 433
            E+W GV Y +AA+MI E +T   FRTAEG + T W  + LG  FQTPEA+     +RSL
Sbjct: 809 DEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRSL 868

Query: 434 CYMRPLAIWAMQWAFSEK 435
            YMRPL+IWAMQ A  ++
Sbjct: 869 AYMRPLSIWAMQLALQQQ 884

[Close]

BLAST of PI0020859 vs. ExPASy Swiss-Prot
Match: Q7KT91 (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.8e-72
Identity = 171/442 (38.69%), Postives = 238/442 (53.85%), Query Frame = 0


Query: 77  GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 136
           G+F YLEG EY M+NTYDVHFY+S A+  L+P L++S+Q DF  A+        ++L +G
Sbjct: 505 GRFGYLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTRKMLYDG 564

Query: 137 KWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVAVW----- 196
           K   R V   VPHD+G  D  P+  +N YN+++ + WKDLN KFVLQ+YRD  V      
Sbjct: 565 KVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYYVLNELAQ 624

Query: 197 ----------------------------------------------------PPVYL--A 256
                                                                 VYL  A
Sbjct: 625 AQSDNASKFSSIEFIDKESLYELYSQDNKRKNSADEKQQNRKSASMYINETNGKVYLMDA 684

Query: 257 IAYMD--------------QFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQ 316
           I Y+               ++D+D DG+IEN   PDQTYD+W + G SAY  GLW+AALQ
Sbjct: 685 IGYLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQ 744

Query: 317 AASALARVADEKDAEHYFWFKFQKAKRAY-EKLWNGSYFNYDSSGGSSSSSIQADQLAGQ 376
           A SA+A + D+ +    +    +K KR+  EKLWNGSY+ +D S  S   +I ADQL G 
Sbjct: 745 AMSAMATILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDLS-HSHRDTIMADQLCGH 804

Query: 377 WYARASGL-LPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPD-------GTVDFS 435
           WY ++ G    I  ++  ++AL ++Y+ NVM   +G  GA NG + +       G VD S
Sbjct: 805 WYLKSCGFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPTKPGHVDNS 864

[Close]

BLAST of PI0020859 vs. ExPASy TrEMBL
Match: A0A1S4E4V1 (non-lysosomal glucosylceramidase-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103502562 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.2e-277
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 371 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 430

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 431 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 490

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 491 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 550

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 551 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 610

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 611 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 670

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 671 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 730

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 731 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 790

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 791 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 850

[Close]

BLAST of PI0020859 vs. ExPASy TrEMBL
Match: A0A1S4E4W0 (non-lysosomal glucosylceramidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502562 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.2e-277
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 409 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 468

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 469 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 528

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 529 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 588

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 589 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 648

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 649 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 708

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 709 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 768

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 769 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 828

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 829 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 888

[Close]

BLAST of PI0020859 vs. ExPASy TrEMBL
Match: A0A1S3CMC1 (Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103502562 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.2e-277
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 463 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 522

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 523 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 582

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 583 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 642

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 643 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 702

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 703 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 762

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 763 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 822

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 823 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 882

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 883 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 942

[Close]

BLAST of PI0020859 vs. ExPASy TrEMBL
Match: A0A1S3CM69 (Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103502562 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.2e-277
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 380 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 439

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 440 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 499

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 500 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 559

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 560 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 619

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 620 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 679

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 680 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 739

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 740 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 799

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 800 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 859

[Close]

BLAST of PI0020859 vs. ExPASy TrEMBL
Match: A0A5A7VAV6 (Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G001100 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.2e-275
Identity = 473/488 (96.93%), Postives = 475/488 (97.34%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 450 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 509

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 510 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 569

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 570 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 629

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 630 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 689

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 690 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 749

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 750 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 809

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 810 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 869

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 486
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 870 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 929

[Close]

BLAST of PI0020859 vs. NCBI nr
Match: XP_016903258.1 (PREDICTED: non-lysosomal glucosylceramidase-like isoform X4 [Cucumis melo])

HSP 1 Score: 962.6 bits (2487), Expect = 1.3e-276
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 371 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 430

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 431 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 490

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 491 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 550

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 551 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 610

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 611 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 670

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 671 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 730

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 731 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 790

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 791 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 850

[Close]

BLAST of PI0020859 vs. NCBI nr
Match: XP_016903257.1 (PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 962.6 bits (2487), Expect = 1.3e-276
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 409 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 468

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 469 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 528

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 529 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 588

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 589 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 648

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 649 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 708

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 709 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 768

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 769 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 828

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 829 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 888

[Close]

BLAST of PI0020859 vs. NCBI nr
Match: XP_008464754.1 (PREDICTED: non-lysosomal glucosylceramidase-like isoform X3 [Cucumis melo])

HSP 1 Score: 962.6 bits (2487), Expect = 1.3e-276
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 380 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 439

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 440 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 499

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 500 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 559

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 560 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 619

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 620 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 679

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 680 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 739

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 740 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 799

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 800 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 859

[Close]

BLAST of PI0020859 vs. NCBI nr
Match: XP_008464753.1 (PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 962.6 bits (2487), Expect = 1.3e-276
Identity = 475/490 (96.94%), Postives = 477/490 (97.35%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 463 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 522

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 523 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 582

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 583 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 642

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 643 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 702

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 703 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 762

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 763 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 822

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 823 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 882

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 487
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 883 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 942

[Close]

BLAST of PI0020859 vs. NCBI nr
Match: KAA0065432.1 (non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 958.4 bits (2476), Expect = 2.4e-275
Identity = 473/488 (96.93%), Postives = 475/488 (97.34%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVN 67
           QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVN
Sbjct: 450 QSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVN 509

Query: 68  LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 127
           LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP
Sbjct: 510 LLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDP 569

Query: 128 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV- 187
           SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 
Sbjct: 570 SKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVV 629

Query: 188 ---------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 247
                    AVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV
Sbjct: 630 ATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWV 689

Query: 248 AALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 307
           AALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL
Sbjct: 690 AALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQL 749

Query: 308 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 367
           AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR
Sbjct: 750 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 809

Query: 368 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 427
           EIWSGVTYAVAASMIHENMTDMAFRTA+GIHE AWSEDGLGYNFQTPEAWTTTDRYRSLC
Sbjct: 810 EIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLC 869

Query: 428 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 486
           YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT
Sbjct: 870 YMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQT 929

[Close]

BLAST of PI0020859 vs. TAIR 10
Match: AT5G49900.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 692.2 bits (1785), Expect = 3.0e-199
Identity = 335/496 (67.54%), Postives = 400/496 (80.65%), Query Frame = 0


Query: 9   SLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNL 68
           SL  + E+ F LD+ + GLK+ I   H+NDTA  +L +M S L+EL  S  SNSAFG  L
Sbjct: 459 SLAGVREKKFSLDKSQLGLKNDIDVPHQNDTAVSVLEKMASTLEELHASTTSNSAFGTKL 518

Query: 69  LQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPS 128
           L++GEEN+G FLYLEG+EY MWNTYDVHFY+SFA++MLFPKLELSIQRDFAAAVM+HDP+
Sbjct: 519 LEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPT 578

Query: 129 KMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV-- 188
           K++ L  G+W  R VLGAVPHD+G+NDPWFEVNGY L+NTDRWKDLNPKFVLQ+YRDV  
Sbjct: 579 KVKTLSEGQWVQRKVLGAVPHDLGINDPWFEVNGYTLHNTDRWKDLNPKFVLQVYRDVVA 638

Query: 189 --------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVA 248
                   AVWP VY+A+AYM QFD+DGDGMIEN+GFPDQTYDTWS +GVSAY GGLWVA
Sbjct: 639 TGDKKFASAVWPSVYVAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVA 698

Query: 249 ALQAASALARVADEKDAEHYFWFKFQKAKRAYE-KLWNGSYFNYDSSGGSSSSSIQADQL 308
           ALQAASALARV  +K+++ YFW KFQKAK  YE KLWNGSYFNYD+SG   SS+IQADQL
Sbjct: 699 ALQAASALARVVGDKNSQDYFWSKFQKAKVVYEKKLWNGSYFNYDNSGSQYSSTIQADQL 758

Query: 309 AGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSR 368
           AGQWYARASGLLPIVDEDKA++AL KVYNYNVMK+KDGKRGAVNGM P+G VD +SMQSR
Sbjct: 759 AGQWYARASGLLPIVDEDKARTALEKVYNYNVMKIKDGKRGAVNGMHPNGKVDTASMQSR 818

Query: 369 EIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLC 428
           EIWSGVTYA++A+MI E + +MAF+TA GI+E AWSE GLGY+FQTPE+W T D YRSL 
Sbjct: 819 EIWSGVTYALSATMIQEGLVEMAFQTASGIYEAAWSETGLGYSFQTPESWNTVDEYRSLT 878

Query: 429 YMRPLAIWAMQWAFSEKVSVIEEL--------KELDSDAILRHHAKFSKVARLLKLPEDG 486
           YMRPLAIWAMQWA ++     E+L         EL+  + ++H   FS+V+RLL LP + 
Sbjct: 879 YMRPLAIWAMQWALTKTSQKQEQLGLEPEQQEPELEPSSSMKHDIGFSRVSRLLSLPNEA 938

[Close]

BLAST of PI0020859 vs. TAIR 10
Match: AT1G33700.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 586.6 bits (1511), Expect = 1.8e-167
Identity = 298/494 (60.32%), Postives = 360/494 (72.87%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSV-IYESHENDTANDILGRMTSRLDELRDSVASNSAFGV 67
           QSL SIG R   L     GL ++   +  +N+ A DILGR+ +   ++   + SN+A G 
Sbjct: 455 QSLDSIGRRKISL-----GLSTIDKNDQDQNNVALDILGRIDAVCSQIHAPLTSNAALGN 514

Query: 68  NLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHD 127
            ++Q   EN+GQFLYLEGV+Y M+NTYDVHFYSSFA++MLFPK+ELSIQRDFAAAV+MHD
Sbjct: 515 TMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHD 574

Query: 128 PSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 187
            SK Q++ +G++  R VLGAVPHDIG+NDPWFE+N YNL+NTDRWKDLN KFVLQ+YRDV
Sbjct: 575 SSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDV 634

Query: 188 ----------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLW 247
                     AVWP VY AIAY+DQFD+DGDGMIEN+GFPDQTYD WS +GVSAY GGLW
Sbjct: 635 VATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLW 694

Query: 248 VAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQ 307
           VAALQA SALAR   +  A  YF  K++KA+  YEKLWNGSYFNYD+S   SSSSI ADQ
Sbjct: 695 VAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNGSYFNYDNSRSGSSSSILADQ 754

Query: 308 LAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQS 367
           +AGQWYARA GL PI  E+  K AL  VY++NVM+V+DG RGAVNGMLPDG VD S+M S
Sbjct: 755 MAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVS 814

Query: 368 REIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSL 427
           RE+W+G TY+VAA MI E + D  FRTA GI+E AWS+ GLG  FQTPEAWTT D YRSL
Sbjct: 815 REVWAGTTYSVAACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSL 874

Query: 428 CYMRPLAIWAMQWAFS------EKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGT 485
           CYMRPLAIW +QWA +      E+   +   +E  +  + + HA F KVA  LK  +   
Sbjct: 875 CYMRPLAIWGIQWAHTMPKPNREQEVSLRPQEEDATSVLFQQHAGFIKVAHYLKNTKGKD 934

[Close]

BLAST of PI0020859 vs. TAIR 10
Match: AT1G33700.2 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 586.6 bits (1511), Expect = 1.8e-167
Identity = 298/494 (60.32%), Postives = 360/494 (72.87%), Query Frame = 0


Query: 8   QSLVSIGERGFCLDQCRSGLKSV-IYESHENDTANDILGRMTSRLDELRDSVASNSAFGV 67
           QSL SIG R   L     GL ++   +  +N+ A DILGR+ +   ++   + SN+A G 
Sbjct: 455 QSLDSIGRRKISL-----GLSTIDKNDQDQNNVALDILGRIDAVCSQIHAPLTSNAALGN 514

Query: 68  NLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHD 127
            ++Q   EN+GQFLYLEGV+Y M+NTYDVHFYSSFA++MLFPK+ELSIQRDFAAAV+MHD
Sbjct: 515 TMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHD 574

Query: 128 PSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV 187
            SK Q++ +G++  R VLGAVPHDIG+NDPWFE+N YNL+NTDRWKDLN KFVLQ+YRDV
Sbjct: 575 SSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDV 634

Query: 188 ----------AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLW 247
                     AVWP VY AIAY+DQFD+DGDGMIEN+GFPDQTYD WS +GVSAY GGLW
Sbjct: 635 VATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLW 694

Query: 248 VAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQ 307
           VAALQA SALAR   +  A  YF  K++KA+  YEKLWNGSYFNYD+S   SSSSI ADQ
Sbjct: 695 VAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNGSYFNYDNSRSGSSSSILADQ 754

Query: 308 LAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQS 367
           +AGQWYARA GL PI  E+  K AL  VY++NVM+V+DG RGAVNGMLPDG VD S+M S
Sbjct: 755 MAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVS 814

Query: 368 REIWSGVTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSL 427
           RE+W+G TY+VAA MI E + D  FRTA GI+E AWS+ GLG  FQTPEAWTT D YRSL
Sbjct: 815 REVWAGTTYSVAACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSL 874

Query: 428 CYMRPLAIWAMQWAFS------EKVSVIEELKELDSDAILRHHAKFSKVARLLKLPEDGT 485
           CYMRPLAIW +QWA +      E+   +   +E  +  + + HA F KVA  LK  +   
Sbjct: 875 CYMRPLAIWGIQWAHTMPKPNREQEVSLRPQEEDATSVLFQQHAGFIKVAHYLKNTKGKD 934

[Close]

BLAST of PI0020859 vs. TAIR 10
Match: AT4G10060.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 545.0 bits (1403), Expect = 5.9e-155
Identity = 271/475 (57.05%), Postives = 335/475 (70.53%), Query Frame = 0


Query: 28  KSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEY 87
           +S +  + +ND   D+  ++ +  +++    +SNS          EEN+GQF+YLEG+EY
Sbjct: 454 RSKVTNTEQNDIVIDLFQKINAVCEQIYSPQSSNS----------EENIGQFIYLEGIEY 513

Query: 88  TMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAV 147
            M+NTYDVHFYSSFA++ LFPKL LSIQRDFAA V++ DP+K +++ +G+W  R +LG+V
Sbjct: 514 LMYNTYDVHFYSSFALLSLFPKLALSIQRDFAATVLIQDPTKKKIMSSGEWVTRKLLGSV 573

Query: 148 PHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDV----------AVWPPVYLAIA 207
           PHDIG+NDPW E+N YN +NTDRWKDLN KFVLQ+YRDV          AVWP VY A+A
Sbjct: 574 PHDIGLNDPWLELNEYNFFNTDRWKDLNAKFVLQVYRDVVATNDQSFAKAVWPSVYTAVA 633

Query: 208 YMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQAASALARVADEKDAEH 267
           Y+DQFD+D DGMIEN+GFPDQTYD WSVTGVSAY GGLWVAALQAASA A +  E     
Sbjct: 634 YLDQFDKDEDGMIENEGFPDQTYDAWSVTGVSAYCGGLWVAALQAASAFASIVGENAVAI 693

Query: 268 YFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKA 327
           YF  K++KAK  YEKLWNGSYFNYD SG  SSSSI ADQLAGQWYARA GL PI  E+  
Sbjct: 694 YFNAKYEKAKIVYEKLWNGSYFNYDDSGSGSSSSILADQLAGQWYARACGLKPITKEEWI 753

Query: 328 KSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSREIWSGVTYAVAASMIHENMT 387
           K AL  +Y +NVMKVK G RGAVNGM  +G VD +S+ S+E+W+G TY+VAA MI E   
Sbjct: 754 KKALETIYEFNVMKVKGGTRGAVNGMSTEGKVDTNSLVSKEVWAGTTYSVAACMIQEGQR 813

Query: 388 DMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSV 447
           +  F+TA GI+E  WS+ GL  +FQTPEAW   D YRSLCYMRPLAIWA+QWA +   S 
Sbjct: 814 EKGFQTASGIYEAIWSDRGLSCSFQTPEAWNMNDEYRSLCYMRPLAIWAIQWALTRTQSF 873

Query: 448 IEELKEL-------DSDAILRHHAKFSKVARLLKL-PEDGTSSSVLQTVYDYTLK 485
            EE ++L       +S+ +LR H  F  VAR +K+ P      S LQ  Y+  LK
Sbjct: 874 GEEKQKLVAGDEEEESNLLLRQHKGFKDVARFVKIVPTSNVHRSRLQHTYETVLK 918

[Close]

BLAST of PI0020859 vs. TAIR 10
Match: AT3G24180.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 5.4e-132
Identity = 234/404 (57.92%), Postives = 289/404 (71.53%), Query Frame = 0


Query: 74  ENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLL 133
           ++VG+FLYLEGVEY MW TYDVHFY+S+A++MLFPK+EL+IQRDFA AV+  D  K++ L
Sbjct: 532 DDVGRFLYLEGVEYVMWCTYDVHFYASYALLMLFPKIELNIQRDFAKAVLSEDGRKVKFL 591

Query: 134 DNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVA------ 193
             G    R V GAVPHD+G++DPW E+N YN+++T RWKDLNPKFVLQ+YRD A      
Sbjct: 592 AEGNVGIRKVRGAVPHDLGMHDPWNEMNAYNIHDTSRWKDLNPKFVLQVYRDFAATGDYQ 651

Query: 194 ----VWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQAA 253
               VWP V  A+ YM+QFDRD D +IENDGFPDQTYDTW+V GVSAY G LW+AALQAA
Sbjct: 652 FGIDVWPAVRAAMEYMEQFDRDNDDLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAA 711

Query: 254 SALARVADEKDAEHYFWFKFQKAKRAYE-KLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 313
           +A+A    +K        KF  AK A E KLWNGSYFNYDS   S+S SIQ DQLAGQWY
Sbjct: 712 AAMALQIGDKFFAELCKNKFLNAKAALETKLWNGSYFNYDSGSSSNSKSIQTDQLAGQWY 771

Query: 314 ARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSREIWSG 373
           A +SGL P+ +E K +S + K++++NVMK K GK GAVNGM PDG VD + MQSREIW+G
Sbjct: 772 AASSGLPPLFEESKIRSTMQKIFDFNVMKTKGGKMGAVNGMHPDGKVDDTCMQSREIWTG 831

Query: 374 VTYAVAASMIHENMTDMAFRTAEGIHETAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPL 433
           VTYA AA+MI   M +  F TAEGI    WSE+G GY FQTPE WT    YRSL YMRPL
Sbjct: 832 VTYAAAATMILSGMEEQGFTTAEGIFTAGWSEEGFGYWFQTPEGWTMDGHYRSLIYMRPL 891

Query: 434 AIWAMQWAFSEKVSVIE--ELKELDSDAILRHHAKFSKVARLLK 465
           AIW MQWA S   ++++  ++  +D   +     +FS   +++K
Sbjct: 892 AIWGMQWALSLPKAILDAPQINMMDRVHLSPRSRRFSNNFKVVK 935

The following BLAST results are available for this feature:

BLAST of PI0020859 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69ZF3	1.4e-87	45.45	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2	[more]
Q9HCG7	3.0e-87	44.64	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2	[more]
Q5M868	3.4e-86	46.30	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2	[more]
Q7KT91	2.8e-72	38.69	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 P...	[more]

BLAST of PI0020859 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E4V1	6.2e-277	96.94	non-lysosomal glucosylceramidase-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S4E4W0	6.2e-277	96.94	non-lysosomal glucosylceramidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3CMC1	6.2e-277	96.94	Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103502562 PE=3 SV...	[more]
A0A1S3CM69	6.2e-277	96.94	Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103502562 PE=3 SV...	[more]
A0A5A7VAV6	1.2e-275	96.93	Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of PI0020859 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016903258.1	1.3e-276	96.94	PREDICTED: non-lysosomal glucosylceramidase-like isoform X4 [Cucumis melo]	[more]
XP_016903257.1	1.3e-276	96.94	PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Cucumis melo]	[more]
XP_008464754.1	1.3e-276	96.94	PREDICTED: non-lysosomal glucosylceramidase-like isoform X3 [Cucumis melo]	[more]
XP_008464753.1	1.3e-276	96.94	PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo]	[more]
KAA0065432.1	2.4e-275	96.93	non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of PI0020859 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G49900.1	3.0e-199	67.54	Beta-glucosidase, GBA2 type family protein	[more]
AT1G33700.1	1.8e-167	60.32	Beta-glucosidase, GBA2 type family protein	[more]
AT1G33700.2	1.8e-167	60.32	Beta-glucosidase, GBA2 type family protein	[more]
AT4G10060.1	5.9e-155	57.05	Beta-glucosidase, GBA2 type family protein	[more]
AT3G24180.1	5.4e-132	57.92	Beta-glucosidase, GBA2 type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 156..426 e-value: 2.5E-12 score: 48.9
IPR006775	Glycosyl-hydrolase family 116, catalytic region	PFAM	PF04685	DUF608	coord: 77..427 e-value: 1.4E-145 score: 485.0
None	No IPR available	PANTHER	PTHR12654	BILE ACID BETA-GLUCOSIDASE-RELATED	coord: 8..486
None	No IPR available	PANTHER	PTHR12654:SF3	NON-LYSOSOMAL GLUCOSYLCERAMIDASE	coord: 8..486
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 83..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0020859.1	PI0020859.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006680	glucosylceramide catabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0004348	glucosylceramidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds