PI0020761 (gene) Melon (PI 482460) v1

Overview
NamePI0020761
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionlong chain acyl-CoA synthetase 1
Locationchr04: 13859777 .. 13865463 (-)
RNA-Seq ExpressionPI0020761
SyntenyPI0020761
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGTGTTTGTTTTGTGGAATTTGGAGGTGGGGTTTTGATGATCATCATATTAACTCCATTTCCATGGCTGTTTTCTTATCACCACTCCTAAATATCTCCAACTTTCTTTAAAAAACAGGCACCATTTTTCTTCCACAAACTCTTTCTTTTTATACTTTTGTACTAACTTCAATCTTCTATTCATGTACTTTAACTCTCTCTCTCACTTCTAGACTTATATTTTATTCATATTCTCAACGAATCTTCCACTTCTGGGTTTGGAGATTAGTACAAGTCTAACGAGTAATCATCCATATTAATCAAGAAGGAATGATGTTTTGTTCATATTCTTGACGCATCTCTCACTTCTGTGCTTAGCAATTAGTACGAGTCAAACGAGTAATTGTCGAAAGATATTAATTGAGAATATTTGTTTGATACTTTCCCCTGCTTTGTTTCCTCCTTCAATTTCACAGATTAAAGCCTAATGACTCTGCTTTTAGTTAACTTCAACACCATTTCCATGGTTTTAGAATTTCTCATCTTAAAAATCTCAACTTTCGAAATGTACCCATTCAACCTAATGTGCTTCTGTTATGACTCTCTGTTTTTTTTCCTTTTTTTTTTCCTTTAAAACTCCATAAAATTTGAGCCATTTTTATTTATTTGTGTCCAATTGGAAGCATTTGGCCATGGTGGTTGGCCTCCCATTAATATCTGCAACTTGGTAGCTGACTTAATAACTCTCTGTACATGTTATATGTAGAACTTTACCCTTCTAATTAATTCTTCAGAGAAATCTTTTGTTTAGGTTTTTGTTTTATTTTTCTTTTTTCCTTCCTATTGAAAGTTTCAATGCAAGTTGTGGAGTCAATGGTGGGACAGTTTTGACATTTCGATCTTTTGAAAAACAGTATATAGCTTTGCTTGTGTATCATTAATCAATATACAACAGGTTTTCCAGCAGTTGAAAATGAAGAGGTTTTCTGTCAAGGTTGAGGAAGGAAGGGAAGGTAAAGACGGCAAACCATCGGTTGGACCGGTTTATCGAAATGTTCTTGCAGAAAAGGAATTTCCTTCTCTGTATGAGGATTTGAGCACATCTTGGGATCTTTTCAGGTTATATTCTATCCATGTTGATGTTCATGTTTTGGGATTCAAATGATTGTGTGAAATGAATGGTTTGATGTTATTGCAAGGCTGATATAGAACATCTTGTGTTTCAAATAAACTTAAGAATGTTAAGGATTTTTTCTTTGTTGGAAACTGCTGAGATAATGAATTCAAGTTGGTTTCTTGGATTGTTTTGCAGTGTTTCAGCTAAAAAGCATGCTGAAAGACGGATGCTCGGATGGCGAAAATTCGTTGACGGGAAGGTGAGTTCAATTCTCATTTTCTATATGAAAGTACAAAAGATAGAAACAATGTTAATGAACTCGAATGGTTTTGGTGATTGCTTGACATTATGATGATTTGATTTGGGTTTCTTTCTTTTTTGGTATCCAGCCAGGACCTTATATATGGAAAACGTACAAAGAAGTTTATGGTGATGTTCTTAACATTGGCTCTGCACTACGAGCAATTGGTGCCGAACCAGTAAGCACACTCCTGCAATTTTAAGGTTTTTCCCATATGATATGGGAGAAGCATATTAATTGTAACAACGGCAAAACTAAAGAAACGGCAATGTTGTGCAACAGGGGTCACGTATCGGGATTTATGGGGCGAACTGTCCTCAATGGATAGTTGCAATGGAGGTTCAACTGAAAGATTCTTCATATTTGTGCTGAATATTCCATAAGCTGTTAAAGTTACTTGTAGTTGTTTGATTGTATAAGATAATAGATGTTCTAAATGTTTTGTGTAGGCTTGTAATGCCCACAGTTTGATTTGTGTGCCTCTCTATGATACTCTTGGTAAAATACTTTTCTGAATTCACTTTTCTAGGAGATTTTAATTTGATGCATTTTCTTATCAGTTAACTTTTTCTGCTTTTTGTAAGTAATTACTTCAAGTTGAGCTTCCTGTTGATTCTTAAAATAATAGAGAAGGGACTAAATGAATTGCAGGACATGGAGCTGTTAATTTCATTATAGATCACGCAGAGATCGACATCGTTTTCATCCAAGATAAGAAAGCAAAAGAAGTAAGAGGGCAGTCTAGTTAATATAATATGCTTTCACATCTCAATCTAAACCAAAAAAGATGCACTCACTAGATTTGGTTGAAATTCTCTCATTTTGCAGCTATTAAATTCTGAATACACAATTCCTGAGAGGCTGAAAGGTGAGAATGATGATATAAGATGGTTTTCTATAGCAGTAATCGAACAAATTTATTGAGTTTGATTCCGTTTCGCAGTTGTTATCTGCTTTACTTCACTCAAGGAGGAAGAAAAGATCTCAGCAACAAACAATGGGATGAAACCATACTCGTGGGACGAGTTCCTTCGCTTGGTCAGTTTTAAATCTAAACCAACTCTTTTATTATCTTCCTTTAATTTATATTTTAGAGTTGACATGTGAATCTATTTGAATAATCAGGGAAAAGAAAATCCATCTGAGATCAGTCCTCCACGACCTCTTGACATTTGCACGATTATGTACACAAGTGGAACCAGCGGAACTCCCAAAGGGGTTGTGTTAACACAAGAAACCATCACTACATTTATTAGAGGAGTTGATATCCATATGGAGCAGTTTGAAGATAAGGTGAACAACTACCCTTATTTTCTTTTAACTTTCAAAGTTGTTGGAATGCCTTAAATTTGATATTAGTGCTCATTGTTATTCATATTTGTCATATTTACAGTATTTATAATTTTGCCCCTAGGGTTTAAAGAATTGTGTTATATAAAGTCTCTAACTTTAAAGGTGTCTCTAATCTGTAATCTTAATTATTCTCTCTAATAATAAATTGTTCTTCCTCTATCTGTGGATGTAGTTAACACACCGTTAATGAAGCACGTAAATCTTTATGCCAATTCTCTACTATCTACCTTTTTCGGCTTATCTCTATATGTCAATTTCATAACAAAAGTCCTTTCCTTTTGCTTATAACATGTATATCTTTCTTGTCAACAGATGACAGAGGATGATGTGTATCTATCATTTCTGCCTCTAGCTCACATCCTCGACCGTACGGTCGAGGAATATTTCTTTCGTAAAGGCGCATCGGTTGGCTACTACCATGGGGTATTATATTCTTTATAGCATCATCTAGCATCTACTTTTGAATAATATCATTCAGATGTTTGATTTACTAATGTTATTTTTTTAAGAGTAAATGATTTGAAATATCTTTTAGACCTTTGAGAATCAGAACAGCTCATTTTCATTTCTCATTCCTTTTATGAAGGATCTTAATGCAATAAAAGATGATCTAATGGAGTTAAAGCCAACGCTTTTAGTCGGCGTACCTCGAGTCTTTGAAAAGATTCACGAAGGCACGAAAACATTGCTCATTATCTGTAATTTCTTTATGTTCCATAACCTTGTTAACAAGTTTAAATTATCAAATCTGTATTTCAGGAATCAAACAAGCTGTACAAGTACTCAATCCAGTGAGAAGGAAGGCTTTTGATTTGCTCTACAAATAGTAAATCAAGTTTTATTCTCTTTTTTACTAATGGTTGCTTATTATCTTCATGATTCTTGTAAAATCAGAGATAAATCATATATATGCTGAACTTGCAGCAAACTTTCATGGATGAATAGTGGATACAAACAAAAAGATGCATCCCCTTTGGCAGATTTATTGGCATTCAGGAAGGTCGAATCCTACTCTCCTAATTAAATTATGTACTCTCGATTACGAGTTTCTCGGCCTCAAAGACATATGTTTGTGTTCTCAAAAAGTCATTTCAAACAAATCCTGAACTTCATATCATACATGTCCATCACTAACTTGATTATCACCTAACACTAAACTTCTATCAACTCAGGTGAAAGCAAGATTAGGTGGTCGCCTTCGACTAATAATATCTGGGGGTGCGGCCTTGAGCACAGAAGTGGAAGAGTTTCTAAGGGTCACATGTTGTGCCTTTTTTGTGCAAGGCTATGGTAAAGAAACACTCTACCTACACAAACAATATAGGTTTAGTTTTACTGATAGCTTTTTAGCACATTTCAAGCTTAAAGATTCTCTATTCTTCGCCTTAATATGCTTCACGAGTTTCTTCTCCGTTGTTTTTGCTATATTGTTTAAAATTTTGTCCAAACATTTTAAACAGCCATGATATGGTAACATTTAGGATTGACTGAAACTTGTGGACCAACAACTATTGGCTTTCCTGATGAGATGTGTATGCTCGGAAATGTTGGCGGCGTTACGCTATTCAATGAGCTTCGTTTGGAGGAGGTTCCGGATATGGGTTACAATCCGCTCGGGGATCGTCCATGTGGCGAAATATGCGTGAGAGGAAAGACTGTTTTTACTGAATACTACAAAGACCCTGAACTAACCAAAGAATCAATAAAGGATGGTTGGTTTCACACAGGTGATTCATCACTTCCCTCTCTTGTTCTTTTATGAAAATTCATTGCTATTTGACTTGTTTATTCCATTTCATTCTTGTACTACTTTAGGAGACATCGGAGAAATGTTTCCCAATGGGGTTGTGAAGATCATTGACAGGAAGAAGAATCTCATAAAACTATCCCAAGGAGAATACATAGCACTCGAATACTTAGAAAATGTTTACTCGATTACCCCAATCGTCGAGGATGTAAGTTTCGAATTGTCTTTCTTTTGAGTTTCTTTCTGAAACTAACTTCATAATATTGATTTAATCTACTCTTTCCAAAGATATGGATCTATGGGAACAGCTTCAAATCAATGCTGGTGGCAGTGGTGGTTCTACATGAGGAAAACACTAAAAAATGGGCAAATTCAAATGGTTTTTTGTGTTCTTTCTCTGAGCTTTGTCCTCTTGAACAGCTTAGACATTATGTTCTCTCTGAGCTCACTTCCACAGCTGAAAGGAACAAGGTATTGCATTTTCTTAAACAACCCAAACCTCGGCTCTACTTCAGTGTCGAATAAGTATGATAAAACCACTTGATAATATTTCTTTTTTACATTTCATATTAAAACAAAGTACTTGTATTAGAATGCCTTGAATTTGTTATTTAACGCTTCGAAATGACCAAGCATAGGGGTCTTGAGGGCAATGATCTTGAAACCTAAGTTTTATTCGTATTTATCTTATTTACGTTATTTATGATTTTGGTCTTAAAGTTTAGAGGGGTGTGCTATATAAACTCTAACTCTTCTGATGCCTCTAAATCTGTAGTTTAATATTTTTCTCTCTAATAAAAAACTGTCCTCTCCCTCTATCCGTAGACGTAGCTAACACACTGTTGATGAATCATGAAAATTTTTGTGATGATTCTCAATTGTTTACATTTTTCTGTATATCTTTTATCGTTGATTTCTTAACTGTTTTAACTCAATGTCCATCAGTTGAAACGTTTCGAATACATCAAAGGAGTTGTGTTGGAATCGCACCCATTCGACATCGAAAAGGATCTGGTGACAGCAACTTTGAAGAAGAAGAGAAACAACCTACTCAAACACTATGAGGTTTGTTGGAAAACTCTTCTTTTTTCATTGATTGAATTCTCCAAAGCATAGGTGAATGAAAAGCTCCCTTCTGTTCTAGGTGCAAATCAATGCAGAGTACAAAAATTTGGAGACAAAGATATGA

mRNA sequence

TCTGTGTTTGTTTTGTGGAATTTGGAGGTGGGGTTTTGATGATCATCATATTAACTCCATTTCCATGGCTGTTTTCTTATCACCACTCCTAAATATCTCCAACTTTCTTTAAAAAACAGGCACCATTTTTCTTCCACAAACTCTTTCTTTTTATACTTTTGTACTAACTTCAATCTTCTATTCATGTACTTTAACTCTCTCTCTCACTTCTAGACTTATATTTTATTCATATTCTCAACGAATCTTCCACTTCTGGGTTTGGAGATTAGTACAAGTCTAACGAGTAATCATCCATATTAATCAAGAAGGAATGATGTTTTGTTCATATTCTTGACGCATCTCTCACTTCTGTGCTTAGCAATTAGTACGAGTCAAACGAGTAATTGTCGAAAGATATTAATTGAGAATATTTGTTTGATACTTTCCCCTGCTTTGTTTCCTCCTTCAATTTCACAGATTAAAGCCTAATGACTCTGCTTTTAGTTAACTTCAACACCATTTCCATGGTTTTAGAATTTCTCATCTTAAAAATCTCAACTTTCGAAATGTACCCATTCAACCTAATGTGCTTCTGTTATGACTCTCTGTTTTTTTTCCTTTTTTTTTTCCTTTAAAACTCCATAAAATTTGAGCCATTTTTATTTATTTGTGTCCAATTGGAAGCATTTGGCCATGGTGGTTGGCCTCCCATTAATATCTGCAACTTGGTAGCTGACTTAATAACTCTCTGTACATGTTATATGTAGAACTTTACCCTTCTAATTAATTCTTCAGAGAAATCTTTTGTTTAGGTTTTTGTTTTATTTTTCTTTTTTCCTTCCTATTGAAAGTTTCAATGCAAGTTGTGGAGTCAATGGTGGGACAGTTTTGACATTTCGATCTTTTGAAAAACAGTATATAGCTTTGCTTGTGTATCATTAATCAATATACAACAGGTTTTCCAGCAGTTGAAAATGAAGAGGTTTTCTGTCAAGGTTGAGGAAGGAAGGGAAGGTAAAGACGGCAAACCATCGGTTGGACCGGTTTATCGAAATGTTCTTGCAGAAAAGGAATTTCCTTCTCTGTATGAGGATTTGAGCACATCTTGGGATCTTTTCAGTGTTTCAGCTAAAAAGCATGCTGAAAGACGGATGCTCGGATGGCGAAAATTCGTTGACGGGAAGCCAGGACCTTATATATGGAAAACGTACAAAGAAGTTTATGGTGATGTTCTTAACATTGGCTCTGCACTACGAGCAATTGGTGCCGAACCAGGGTCACGTATCGGGATTTATGGGGCGAACTGTCCTCAATGGATAGTTGCAATGGAGGCTTGTAATGCCCACAGTTTGATTTGTGTGCCTCTCTATGATACTCTTGGACATGGAGCTGTTAATTTCATTATAGATCACGCAGAGATCGACATCGTTTTCATCCAAGATAAGAAAGCAAAAGAACTATTAAATTCTGAATACACAATTCCTGAGAGGCTGAAAGTTGTTATCTGCTTTACTTCACTCAAGGAGGAAGAAAAGATCTCAGCAACAAACAATGGGATGAAACCATACTCGTGGGACGAGTTCCTTCGCTTGGGAAAAGAAAATCCATCTGAGATCAGTCCTCCACGACCTCTTGACATTTGCACGATTATGTACACAAGTGGAACCAGCGGAACTCCCAAAGGGGTTGTGTTAACACAAGAAACCATCACTACATTTATTAGAGGAGTTGATATCCATATGGAGCAGTTTGAAGATAAGATGACAGAGGATGATGTGTATCTATCATTTCTGCCTCTAGCTCACATCCTCGACCGTACGGTCGAGGAATATTTCTTTCGTAAAGGCGCATCGGTTGGCTACTACCATGGGGATCTTAATGCAATAAAAGATGATCTAATGGAGTTAAAGCCAACGCTTTTAGTCGGCGTACCTCGAGTCTTTGAAAAGATTCACGAAGGAATCAAACAAGCTGTACAAGTACTCAATCCAGTGAGAAGGAAGGCTTTTGATTTGCTCTACAAATACAAACTTTCATGGATGAATAGTGGATACAAACAAAAAGATGCATCCCCTTTGGCAGATTTATTGGCATTCAGGAAGGTGAAAGCAAGATTAGGTGGTCGCCTTCGACTAATAATATCTGGGGGTGCGGCCTTGAGCACAGAAGTGGAAGAGTTTCTAAGGGTCACATGTTGTGCCTTTTTTGTGCAAGGCTATGGATTGACTGAAACTTGTGGACCAACAACTATTGGCTTTCCTGATGAGATGTGTATGCTCGGAAATGTTGGCGGCGTTACGCTATTCAATGAGCTTCGTTTGGAGGAGGTTCCGGATATGGGTTACAATCCGCTCGGGGATCGTCCATGTGGCGAAATATGCGTGAGAGGAAAGACTGTTTTTACTGAATACTACAAAGACCCTGAACTAACCAAAGAATCAATAAAGGATGGTTGGTTTCACACAGGAGACATCGGAGAAATGTTTCCCAATGGGGTTGTGAAGATCATTGACAGGAAGAAGAATCTCATAAAACTATCCCAAGGAGAATACATAGCACTCGAATACTTAGAAAATGTTTACTCGATTACCCCAATCGTCGAGGATATATGGATCTATGGGAACAGCTTCAAATCAATGCTGGTGGCAGTGGTGGTTCTACATGAGGAAAACACTAAAAAATGGGCAAATTCAAATGGTTTTTTGTGTTCTTTCTCTGAGCTTTGTCCTCTTGAACAGCTTAGACATTATGTTCTCTCTGAGCTCACTTCCACAGCTGAAAGGAACAAGTTGAAACGTTTCGAATACATCAAAGGAGTTGTGTTGGAATCGCACCCATTCGACATCGAAAAGGATCTGGTGACAGCAACTTTGAAGAAGAAGAGAAACAACCTACTCAAACACTATGAGGTGCAAATCAATGCAGAGTACAAAAATTTGGAGACAAAGATATGA

Coding sequence (CDS)

ATGAAGAGGTTTTCTGTCAAGGTTGAGGAAGGAAGGGAAGGTAAAGACGGCAAACCATCGGTTGGACCGGTTTATCGAAATGTTCTTGCAGAAAAGGAATTTCCTTCTCTGTATGAGGATTTGAGCACATCTTGGGATCTTTTCAGTGTTTCAGCTAAAAAGCATGCTGAAAGACGGATGCTCGGATGGCGAAAATTCGTTGACGGGAAGCCAGGACCTTATATATGGAAAACGTACAAAGAAGTTTATGGTGATGTTCTTAACATTGGCTCTGCACTACGAGCAATTGGTGCCGAACCAGGGTCACGTATCGGGATTTATGGGGCGAACTGTCCTCAATGGATAGTTGCAATGGAGGCTTGTAATGCCCACAGTTTGATTTGTGTGCCTCTCTATGATACTCTTGGACATGGAGCTGTTAATTTCATTATAGATCACGCAGAGATCGACATCGTTTTCATCCAAGATAAGAAAGCAAAAGAACTATTAAATTCTGAATACACAATTCCTGAGAGGCTGAAAGTTGTTATCTGCTTTACTTCACTCAAGGAGGAAGAAAAGATCTCAGCAACAAACAATGGGATGAAACCATACTCGTGGGACGAGTTCCTTCGCTTGGGAAAAGAAAATCCATCTGAGATCAGTCCTCCACGACCTCTTGACATTTGCACGATTATGTACACAAGTGGAACCAGCGGAACTCCCAAAGGGGTTGTGTTAACACAAGAAACCATCACTACATTTATTAGAGGAGTTGATATCCATATGGAGCAGTTTGAAGATAAGATGACAGAGGATGATGTGTATCTATCATTTCTGCCTCTAGCTCACATCCTCGACCGTACGGTCGAGGAATATTTCTTTCGTAAAGGCGCATCGGTTGGCTACTACCATGGGGATCTTAATGCAATAAAAGATGATCTAATGGAGTTAAAGCCAACGCTTTTAGTCGGCGTACCTCGAGTCTTTGAAAAGATTCACGAAGGAATCAAACAAGCTGTACAAGTACTCAATCCAGTGAGAAGGAAGGCTTTTGATTTGCTCTACAAATACAAACTTTCATGGATGAATAGTGGATACAAACAAAAAGATGCATCCCCTTTGGCAGATTTATTGGCATTCAGGAAGGTGAAAGCAAGATTAGGTGGTCGCCTTCGACTAATAATATCTGGGGGTGCGGCCTTGAGCACAGAAGTGGAAGAGTTTCTAAGGGTCACATGTTGTGCCTTTTTTGTGCAAGGCTATGGATTGACTGAAACTTGTGGACCAACAACTATTGGCTTTCCTGATGAGATGTGTATGCTCGGAAATGTTGGCGGCGTTACGCTATTCAATGAGCTTCGTTTGGAGGAGGTTCCGGATATGGGTTACAATCCGCTCGGGGATCGTCCATGTGGCGAAATATGCGTGAGAGGAAAGACTGTTTTTACTGAATACTACAAAGACCCTGAACTAACCAAAGAATCAATAAAGGATGGTTGGTTTCACACAGGAGACATCGGAGAAATGTTTCCCAATGGGGTTGTGAAGATCATTGACAGGAAGAAGAATCTCATAAAACTATCCCAAGGAGAATACATAGCACTCGAATACTTAGAAAATGTTTACTCGATTACCCCAATCGTCGAGGATATATGGATCTATGGGAACAGCTTCAAATCAATGCTGGTGGCAGTGGTGGTTCTACATGAGGAAAACACTAAAAAATGGGCAAATTCAAATGGTTTTTTGTGTTCTTTCTCTGAGCTTTGTCCTCTTGAACAGCTTAGACATTATGTTCTCTCTGAGCTCACTTCCACAGCTGAAAGGAACAAGTTGAAACGTTTCGAATACATCAAAGGAGTTGTGTTGGAATCGCACCCATTCGACATCGAAAAGGATCTGGTGACAGCAACTTTGAAGAAGAAGAGAAACAACCTACTCAAACACTATGAGGTGCAAATCAATGCAGAGTACAAAAATTTGGAGACAAAGATATGA

Protein sequence

MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRMLGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEACNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTSLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLTQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYKQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI
Homology
BLAST of PI0020761 vs. ExPASy Swiss-Prot
Match: O22898 (Long chain acyl-CoA synthetase 1 OS=Arabidopsis thaliana OX=3702 GN=LACS1 PE=2 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 3.9e-271
Identity = 426/658 (64.74%), Postives = 537/658 (81.61%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MK F+ KVEEG +G DGKPSVGPVYRN+L+EK FP +  +++T+WD+FS S +K  +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWR+ VD K GPY+WKTYKEVY +VL IGSALRA GAEPGSR+GIYG NCPQWI+AMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VF+QD K K LL  +    +RLK ++ FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           ++ +E    A+  G+K YSW +FL +G+E P + +PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           T + + TF+ G+D++M+QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LN ++DD+ ELKPT L GVPRVFE+IHEGI++A+Q LNP RR  F+ LYK+KL+W+N GY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
               ASP+AD +AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
            G T +GFPDEMCMLG VG   ++NE+RLEEV +MGY+PLG+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELT+E +KDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           +V+DIW+YG+SFKSMLVAVVV + E   +WA   GF   F ELC   +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETK 659
           AE+NKL++FEYIK V +E+ PFD+E+DLVTATLK +RNNLLK+Y+VQI+  Y+ L +K
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASK 658

BLAST of PI0020761 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 2.1e-192
Identity = 312/657 (47.49%), Postives = 445/657 (67.73%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRML 61
           K++  +VEEG+EG DG+PSVGPVYR++ A+  FP   E + + WD+F +S +K+    ML
Sbjct: 5   KKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPML 64

Query: 62  GWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEAC 121
           G R+ VDGKPG Y+W+TY+EVY  V+ +G++LR++G +  ++ GIYGAN P+WI++MEAC
Sbjct: 65  GRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTS 181
           NAH L CVPLYDTLG  AV FII H+E+ IVF+++KK  EL  +     E +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFGG 184

Query: 182 LKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +  E+K  A   G+  Y+WDEFL+LG+    ++   +  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
            E+I T I GV   ++   + +T  DVYLS+LPLAHI DR +EE F + GA++G++ GD+
Sbjct: 245 NESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYK 361
             + +DL ELKPT+   VPRV ++++ G+++ +     +++  FD  + YK  +M  G  
Sbjct: 305 KLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQS 364

Query: 362 QKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
             +ASPL D L F KVK  LGG +R+I+SG A L++ VE FLRV  C   +QGYGLTE+C
Sbjct: 365 HVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYK 481
             T +  PDE+ MLG VG      ++RLE VP+M Y+ L     GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYYK 484

Query: 482 DPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y     
Sbjct: 485 REDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQA 544

Query: 542 VEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 601
           V+ +W+YGNSF+S L+A+   ++   ++WA  NG    +  LC  E+ + ++L EL   A
Sbjct: 545 VDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKMA 604

Query: 602 ERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETK 659
           +  K+K FE IK + L+  PFD+E+DL+T T KKKR  LLK+Y+  I+  YK +  K
Sbjct: 605 KEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINAK 661

BLAST of PI0020761 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 3.0e-191
Identity = 317/658 (48.18%), Postives = 437/658 (66.41%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRML 61
           KRF  +VE  +E  DG PSVGPVYR+  A+  FP+  + + + WD+F  + +K+   RML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 62  GWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEAC 121
           G R+  +GK G Y+WKTYKEVY  V+ +G++LR+ G + G + GIYG NC +WI++MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTS 181
           NAH L CVPLYDTLG GAV FII HAE+ I F+++KK  EL  +     + +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 182 LKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +K E+K  A   G+  +SWDEFL+LG+    E+   +P DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
            E+I T   GV   +      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ GD+
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYK 361
             + +DL ELKP++   VPRV ++++ G++Q +      ++K FD+ + YK   M  G  
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 362 QKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
              ASP  D L F KVK  LGG +R+I+SG A L++ +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYK 481
             T   FPDE+ MLG VG      ++RLE VP+M Y+ LG  P GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 482 DPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVYS   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 542 VEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 601
           +E IW+YGNSF+S LVA+    ++  ++WA  NG    F+ +C   + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

Query: 602 ERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           + NKLK FE IK V LE   FD+E+DL+T T KKKR  LLK+Y+  I+  YK  +  +
Sbjct: 605 KENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKESL 662

BLAST of PI0020761 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 5.7e-190
Identity = 313/652 (48.01%), Postives = 442/652 (67.79%), Query Frame = 0

Query: 3   RFSVKVEEGREGKD-GKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRML 62
           R+ V+VE+G++G D G PSVGPVYR++ A+  FP   +DL ++WD+F +S +K     ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 63  GWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEAC 122
           G R+ VDGK G Y+W+TYKEV+  V+ +G+++R IG   G + GIYGAN P+WI++MEAC
Sbjct: 65  GRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 124

Query: 123 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTS 182
           NAH L CVPLYDTLG GA+ FII HAE+ + F ++ K  ELL +     + LK ++ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 184

Query: 183 LKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLT 242
           +   +++ A  + +  YSWD+FL+LG+    E+   R  D+CTIMYTSGT+G PKGV+LT
Sbjct: 185 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 244

Query: 243 QETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 302
            E+I   + GV   ++  ++++T  DVYLS+LPLAHI DR +EE    + AS+G++ GD+
Sbjct: 245 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 304

Query: 303 NAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYK 362
             + +D+  LKPT+   VPRV E+I+ G++Q +     V++K F+  +KYK   M  G  
Sbjct: 305 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 364

Query: 363 QKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 422
            + ASP+AD + F+KVK  LGG +RLI+SG A L+  +E FLRV  CA  +QGYGLTE+C
Sbjct: 365 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 424

Query: 423 GPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYK 482
           G T +  P+E+ MLG VG      ++RLE VP+MGY+ L   P GEIC+RGKT+F+ YYK
Sbjct: 425 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 484

Query: 483 DPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 542
             +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+YS    
Sbjct: 485 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 544

Query: 543 VEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 602
           +E IW+YGNS++S LVAVV   +   + WA  +     F  +C  ++ + +VL E    A
Sbjct: 545 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 604

Query: 603 ERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYK 654
           +  KLK FE IKGV L++ PFD+E+DL+T + K KR  LLK+Y+ +I+  YK
Sbjct: 605 KDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

BLAST of PI0020761 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 8.1e-176
Identity = 299/649 (46.07%), Postives = 411/649 (63.33%), Query Frame = 0

Query: 4   FSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRMLGW 63
           ++VKVEE R   +  PS GPVYR++ A      L E L + W+  S S K+  +  MLG 
Sbjct: 3   YTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLGR 62

Query: 64  RKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEACNA 123
           R+  D + GPY+W TY+EV+ + + + SA+R+ G  PG R GIYG NCPQWIVAM+AC +
Sbjct: 63  RQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQACYS 122

Query: 124 HSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTSLK 183
           H++  VPLYDTLG  AV FII+H E+ I F+Q+ K   +L+        LK ++ F ++ 
Sbjct: 123 HAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGNIT 182

Query: 184 EEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLTQE 243
             +K      G+  +SW+EF +LG  +  E+      DICT+MYTSG +G PKGV+LT E
Sbjct: 183 SVQKKETEALGVSCFSWEEFSQLGNLS-GELPEKHRTDICTLMYTSGATGEPKGVILTNE 242

Query: 244 TITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNA 303
            I   I   D  +E  +   +E+DVY S+LPLAH+ D+ VE Y   KG+++GY+ GD+  
Sbjct: 243 AIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDIRF 302

Query: 304 IKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYKQK 363
           + DDL ELKPT+  GVPRV+++I+ GI   V     +++  F   Y YK++ M  G    
Sbjct: 303 LMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLPHG 362

Query: 364 DASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETCGP 423
            A+PL D L F K+K   GGR+RL+ SG A L   VEE+LRVT CA   QGYGLTE+CG 
Sbjct: 363 QAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESCGG 422

Query: 424 TTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYKDP 483
                 +   M+G VG      E RLE VP+MGY+ L D+P GEIC+RG T+F+ Y+K  
Sbjct: 423 CLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHKRE 482

Query: 484 ELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPIVE 543
           +LTK+ + DGWFHTGDIGE  PNG +KIIDRKKN+ KLSQGEY+ +E +E +Y   P++ 
Sbjct: 483 DLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPLIA 542

Query: 544 DIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTAER 603
            IW+YGNSF+S LVAVVV      + WA +      F  LC   +   Y+L EL STA++
Sbjct: 543 SIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTAKK 602

Query: 604 NKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEY 653
           ++L+ FE +K V LE +PFD+E+DL+T T K KR  LLK+Y+  ++  Y
Sbjct: 603 HQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLY 650

BLAST of PI0020761 vs. ExPASy TrEMBL
Match: A0A1S4DWM9 (long chain acyl-CoA synthetase 1 OS=Cucumis melo OX=3656 GN=LOC103489907 PE=4 SV=1)

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 643/659 (97.57%), Postives = 651/659 (98.79%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAEKEFPS YEDLSTSWDLFSVSAKK+A+RRM
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDI+FIQDKKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYE QINAEYKNLETKI
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLETKI 659

BLAST of PI0020761 vs. ExPASy TrEMBL
Match: A0A0A0L8J2 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G535120 PE=4 SV=1)

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 646/659 (98.03%), Postives = 649/659 (98.48%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPS YEDLSTSWDLFSVSA K+AERRM
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSANKYAERRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNS  TIPERLKV ICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSGCTIPERLKVFICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGNVGGVTLFNEL LEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELCLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           AERNKLKRFEYIKGVVLE  PFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLE KI
Sbjct: 601 AERNKLKRFEYIKGVVLELRPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLEKKI 659

BLAST of PI0020761 vs. ExPASy TrEMBL
Match: A0A5D3DAJ7 (Long chain acyl-CoA synthetase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00640 PE=4 SV=1)

HSP 1 Score: 1285.8 bits (3326), Expect = 0.0e+00
Identity = 631/659 (95.75%), Postives = 638/659 (96.81%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAEKEFPS             VSAKK+A+RRM
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLL KYKLSWMNSGY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLCKYKLSWMNSGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYE QINAEYKNLETKI
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLETKI 647

BLAST of PI0020761 vs. ExPASy TrEMBL
Match: A0A5A7T933 (Long chain acyl-CoA synthetase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003380 PE=4 SV=1)

HSP 1 Score: 1278.1 bits (3306), Expect = 0.0e+00
Identity = 631/668 (94.46%), Postives = 638/668 (95.51%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAEKEFPS             VSAKK+A+RRM
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEG---------IKQAVQVLNPVRRKAFDLLYKY 360
           LNAIKDDLMELKPTLLVGVPRVFEKIHEG         IKQAVQVLNPVRRKAFDLL KY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGTKALLIICRIKQAVQVLNPVRRKAFDLLCKY 360

Query: 361 KLSWMNSGYKQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420
           KLSWMNSGYKQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF
Sbjct: 361 KLSWMNSGYKQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420

Query: 421 VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR 480
           VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR
Sbjct: 421 VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR 480

Query: 481 GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEY 540
           GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEY
Sbjct: 481 GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEY 540

Query: 541 LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 600
           LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH
Sbjct: 541 LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 600

Query: 601 YVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAE 660
           YVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYE QINAE
Sbjct: 601 YVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAE 656

BLAST of PI0020761 vs. ExPASy TrEMBL
Match: A0A6J1J2W0 (long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482186 PE=4 SV=1)

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 594/658 (90.27%), Postives = 628/658 (95.44%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFS+KVEEGREGKDGKPSVGPVYRNVLA+   PSL+EDL+TSWDLFSVSA+K+A R+M
Sbjct: 1   MKRFSIKVEEGREGKDGKPSVGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVYGDVL+IGSALRA+GA+PGSRIGIYG NCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYGDVLDIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHS+ICVPLYDTLGH AVNFIIDHAEIDIVF+QDKKAKELL S  T P+RLKVV+CFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSGRTNPDRLKVVVCFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISA +NG+KPYSWDEF RLGKENPSEI+PPR LDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           T ETI TFIRGVDI+MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 THETIVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LNAIK+DLMEL+PTL+VGVPRVFE+I EGIKQAVQVLNPVRRKAFDLLYKYKLSWMN GY
Sbjct: 301 LNAIKEDLMELRPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNRGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQK+ASPLAD LAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCAFF+QGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VG VT FN+LRLE+VP+MGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEKVPEMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELTKE+IKDGWFHTGDIGE+ PNGVVKIIDRKKNLIKLSQGEYIALEYLENVYS TP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSNTP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKS LVAVVVLHEENTKKWANSNGFLCSFSEL  LEQLR+YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETK 659
           AERNKLKRFEYIKGVVLE HPFDIE DLVTATLKKKRNNLLKHYEVQIN EYKNLE K
Sbjct: 601 AERNKLKRFEYIKGVVLEPHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAK 658

BLAST of PI0020761 vs. NCBI nr
Match: XP_016900385.1 (PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo] >XP_016900386.1 PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo])

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 643/659 (97.57%), Postives = 651/659 (98.79%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAEKEFPS YEDLSTSWDLFSVSAKK+A+RRM
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDI+FIQDKKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYE QINAEYKNLETKI
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLETKI 659

BLAST of PI0020761 vs. NCBI nr
Match: XP_011651533.1 (long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739343.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739344.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739345.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739346.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >KGN58128.1 hypothetical protein Csa_017503 [Cucumis sativus])

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 646/659 (98.03%), Postives = 649/659 (98.48%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPS YEDLSTSWDLFSVSA K+AERRM
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSANKYAERRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNS  TIPERLKV ICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSGCTIPERLKVFICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGNVGGVTLFNEL LEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELCLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           AERNKLKRFEYIKGVVLE  PFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLE KI
Sbjct: 601 AERNKLKRFEYIKGVVLELRPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLEKKI 659

BLAST of PI0020761 vs. NCBI nr
Match: TYK20490.1 (long chain acyl-CoA synthetase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1285.8 bits (3326), Expect = 0.0e+00
Identity = 631/659 (95.75%), Postives = 638/659 (96.81%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAEKEFPS             VSAKK+A+RRM
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLL KYKLSWMNSGY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLCKYKLSWMNSGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
           KQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYE QINAEYKNLETKI
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLETKI 647

BLAST of PI0020761 vs. NCBI nr
Match: KAA0038117.1 (long chain acyl-CoA synthetase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1278.1 bits (3306), Expect = 0.0e+00
Identity = 631/668 (94.46%), Postives = 638/668 (95.51%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAEKEFPS             VSAKK+A+RRM
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TQETITTFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEG---------IKQAVQVLNPVRRKAFDLLYKY 360
           LNAIKDDLMELKPTLLVGVPRVFEKIHEG         IKQAVQVLNPVRRKAFDLL KY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGTKALLIICRIKQAVQVLNPVRRKAFDLLCKY 360

Query: 361 KLSWMNSGYKQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420
           KLSWMNSGYKQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF
Sbjct: 361 KLSWMNSGYKQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420

Query: 421 VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR 480
           VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR
Sbjct: 421 VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR 480

Query: 481 GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEY 540
           GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGV+KIIDRKKNLIKLSQGEYIALEY
Sbjct: 481 GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEY 540

Query: 541 LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 600
           LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH
Sbjct: 541 LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 600

Query: 601 YVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAE 660
           YVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYE QINAE
Sbjct: 601 YVLSELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAE 656

BLAST of PI0020761 vs. NCBI nr
Match: XP_038903045.1 (LOW QUALITY PROTEIN: long chain acyl-CoA synthetase 1-like [Benincasa hispida])

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 611/665 (91.88%), Postives = 634/665 (95.34%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MKRFSVKVEEGREGKDGKPSV PVYRNVLAE +FPS YED ST+WDLFSVSAKK+AERRM
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVEPVYRNVLAENDFPSPYEDSSTTWDLFSVSAKKYAERRM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWRKFV+GKPGPYIWKTYKEV GDVLNIGSALRAIGAEP S IGIYGA  PQWI+AME 
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVXGDVLNIGSALRAIGAEPVSVIGIYGAKLPQWIIAMEG 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKE------LLNSEYTIPERLK 180
           CNAHSLI +PLYDTLGHGAVNFIIDHAEIDIVF+QDK+ K       LLNSE  IPERLK
Sbjct: 121 CNAHSLIXLPLYDTLGHGAVNFIIDHAEIDIVFVQDKEGKRSKSCCLLLNSESAIPERLK 180

Query: 181 VVICFTSLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGT 240
           VVICFTSLKEEEKISA  NG+KPYSWDEFLR+GKENP E++PPRPL+ICTIMYTSGTSGT
Sbjct: 181 VVICFTSLKEEEKISAAGNGLKPYSWDEFLRMGKENPFEVTPPRPLNICTIMYTSGTSGT 240

Query: 241 PKGVVLTQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASV 300
           PKGVVLTQET+T FIRGVDI+MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASV
Sbjct: 241 PKGVVLTQETLTMFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASV 300

Query: 301 GYYHGDLNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLS 360
           GYYHGDLNAIKDDLMELKPTLLVGVPRVFE+IHEGIKQAVQVLNPVRRKAFDLLYKYKL 
Sbjct: 301 GYYHGDLNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQVLNPVRRKAFDLLYKYKLL 360

Query: 361 WMNSGYKQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQG 420
           WMNSGYKQKDASPLAD LAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF+QG
Sbjct: 361 WMNSGYKQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFIQG 420

Query: 421 YGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKT 480
           YGLTETCGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNPLGDRPCGEICVRGKT
Sbjct: 421 YGLTETCGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPLGDRPCGEICVRGKT 480

Query: 481 VFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLEN 540
           VFTEYYK+PELTKE+IKDGWFHTGDIGE+FPNGVVKIIDRKKNLIKLSQGEYIALEYLEN
Sbjct: 481 VFTEYYKNPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLEN 540

Query: 541 VYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVL 600
           VYS+TPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVL
Sbjct: 541 VYSVTPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVL 600

Query: 601 SELTSTAERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKN 660
           SELTSTAERNKLKRFEYIKGVVLE HPFDIEKDLVTATLKKKRNNLLKH+EVQINAEYKN
Sbjct: 601 SELTSTAERNKLKRFEYIKGVVLELHPFDIEKDLVTATLKKKRNNLLKHFEVQINAEYKN 660

BLAST of PI0020761 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 935.3 bits (2416), Expect = 2.8e-272
Identity = 426/658 (64.74%), Postives = 537/658 (81.61%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MK F+ KVEEG +G DGKPSVGPVYRN+L+EK FP +  +++T+WD+FS S +K  +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWR+ VD K GPY+WKTYKEVY +VL IGSALRA GAEPGSR+GIYG NCPQWI+AMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VF+QD K K LL  +    +RLK ++ FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           ++ +E    A+  G+K YSW +FL +G+E P + +PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           T + + TF+ G+D++M+QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LN ++DD+ ELKPT L GVPRVFE+IHEGI++A+Q LNP RR  F+ LYK+KL+W+N GY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
               ASP+AD +AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
            G T +GFPDEMCMLG VG   ++NE+RLEEV +MGY+PLG+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELT+E +KDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           +V+DIW+YG+SFKSMLVAVVV + E   +WA   GF   F ELC   +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETK 659
           AE+NKL++FEYIK V +E+ PFD+E+DLVTATLK +RNNLLK+Y+VQI+  Y+ L +K
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASK 658

BLAST of PI0020761 vs. TAIR 10
Match: AT2G47240.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 935.3 bits (2416), Expect = 2.8e-272
Identity = 426/658 (64.74%), Postives = 537/658 (81.61%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRM 60
           MK F+ KVEEG +G DGKPSVGPVYRN+L+EK FP +  +++T+WD+FS S +K  +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120
           LGWR+ VD K GPY+WKTYKEVY +VL IGSALRA GAEPGSR+GIYG NCPQWI+AMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VF+QD K K LL  +    +RLK ++ FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240
           ++ +E    A+  G+K YSW +FL +G+E P + +PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 TQETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           T + + TF+ G+D++M+QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360
           LN ++DD+ ELKPT L GVPRVFE+IHEGI++A+Q LNP RR  F+ LYK+KL+W+N GY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
               ASP+AD +AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480
            G T +GFPDEMCMLG VG   ++NE+RLEEV +MGY+PLG+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELT+E +KDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600
           +V+DIW+YG+SFKSMLVAVVV + E   +WA   GF   F ELC   +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETK 659
           AE+NKL++FEYIK V +E+ PFD+E+DLVTATLK +RNNLLK+Y+VQI+  Y+ L +K
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASK 658

BLAST of PI0020761 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 673.7 bits (1737), Expect = 1.5e-193
Identity = 312/657 (47.49%), Postives = 445/657 (67.73%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRML 61
           K++  +VEEG+EG DG+PSVGPVYR++ A+  FP   E + + WD+F +S +K+    ML
Sbjct: 5   KKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPML 64

Query: 62  GWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEAC 121
           G R+ VDGKPG Y+W+TY+EVY  V+ +G++LR++G +  ++ GIYGAN P+WI++MEAC
Sbjct: 65  GRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTS 181
           NAH L CVPLYDTLG  AV FII H+E+ IVF+++KK  EL  +     E +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFGG 184

Query: 182 LKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +  E+K  A   G+  Y+WDEFL+LG+    ++   +  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
            E+I T I GV   ++   + +T  DVYLS+LPLAHI DR +EE F + GA++G++ GD+
Sbjct: 245 NESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYK 361
             + +DL ELKPT+   VPRV ++++ G+++ +     +++  FD  + YK  +M  G  
Sbjct: 305 KLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQS 364

Query: 362 QKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
             +ASPL D L F KVK  LGG +R+I+SG A L++ VE FLRV  C   +QGYGLTE+C
Sbjct: 365 HVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYK 481
             T +  PDE+ MLG VG      ++RLE VP+M Y+ L     GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYYK 484

Query: 482 DPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y     
Sbjct: 485 REDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQA 544

Query: 542 VEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 601
           V+ +W+YGNSF+S L+A+   ++   ++WA  NG    +  LC  E+ + ++L EL   A
Sbjct: 545 VDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKMA 604

Query: 602 ERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETK 659
           +  K+K FE IK + L+  PFD+E+DL+T T KKKR  LLK+Y+  I+  YK +  K
Sbjct: 605 KEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINAK 661

BLAST of PI0020761 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 669.8 bits (1727), Expect = 2.2e-192
Identity = 317/658 (48.18%), Postives = 437/658 (66.41%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRML 61
           KRF  +VE  +E  DG PSVGPVYR+  A+  FP+  + + + WD+F  + +K+   RML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 62  GWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEAC 121
           G R+  +GK G Y+WKTYKEVY  V+ +G++LR+ G + G + GIYG NC +WI++MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTS 181
           NAH L CVPLYDTLG GAV FII HAE+ I F+++KK  EL  +     + +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 182 LKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +K E+K  A   G+  +SWDEFL+LG+    E+   +P DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
            E+I T   GV   +      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ GD+
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYK 361
             + +DL ELKP++   VPRV ++++ G++Q +      ++K FD+ + YK   M  G  
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 362 QKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
              ASP  D L F KVK  LGG +R+I+SG A L++ +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYK 481
             T   FPDE+ MLG VG      ++RLE VP+M Y+ LG  P GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 482 DPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVYS   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 542 VEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 601
           +E IW+YGNSF+S LVA+    ++  ++WA  NG    F+ +C   + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

Query: 602 ERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLETKI 660
           + NKLK FE IK V LE   FD+E+DL+T T KKKR  LLK+Y+  I+  YK  +  +
Sbjct: 605 KENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKESL 662

BLAST of PI0020761 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 665.6 bits (1716), Expect = 4.1e-191
Identity = 313/652 (48.01%), Postives = 442/652 (67.79%), Query Frame = 0

Query: 3   RFSVKVEEGREGKD-GKPSVGPVYRNVLAEKEFPSLYEDLSTSWDLFSVSAKKHAERRML 62
           R+ V+VE+G++G D G PSVGPVYR++ A+  FP   +DL ++WD+F +S +K     ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 63  GWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEAC 122
           G R+ VDGK G Y+W+TYKEV+  V+ +G+++R IG   G + GIYGAN P+WI++MEAC
Sbjct: 65  GRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 124

Query: 123 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSEYTIPERLKVVICFTS 182
           NAH L CVPLYDTLG GA+ FII HAE+ + F ++ K  ELL +     + LK ++ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 184

Query: 183 LKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVLT 242
           +   +++ A  + +  YSWD+FL+LG+    E+   R  D+CTIMYTSGT+G PKGV+LT
Sbjct: 185 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 244

Query: 243 QETITTFIRGVDIHMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 302
            E+I   + GV   ++  ++++T  DVYLS+LPLAHI DR +EE    + AS+G++ GD+
Sbjct: 245 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 304

Query: 303 NAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGYK 362
             + +D+  LKPT+   VPRV E+I+ G++Q +     V++K F+  +KYK   M  G  
Sbjct: 305 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 364

Query: 363 QKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 422
            + ASP+AD + F+KVK  LGG +RLI+SG A L+  +E FLRV  CA  +QGYGLTE+C
Sbjct: 365 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 424

Query: 423 GPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYYK 482
           G T +  P+E+ MLG VG      ++RLE VP+MGY+ L   P GEIC+RGKT+F+ YYK
Sbjct: 425 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 484

Query: 483 DPELTKESIKDGWFHTGDIGEMFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 542
             +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+YS    
Sbjct: 485 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 544

Query: 543 VEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 602
           +E IW+YGNS++S LVAVV   +   + WA  +     F  +C  ++ + +VL E    A
Sbjct: 545 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 604

Query: 603 ERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYK 654
           +  KLK FE IKGV L++ PFD+E+DL+T + K KR  LLK+Y+ +I+  YK
Sbjct: 605 KDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O228983.9e-27164.74Long chain acyl-CoA synthetase 1 OS=Arabidopsis thaliana OX=3702 GN=LACS1 PE=2 S... [more]
Q9T0A02.1e-19247.49Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9T0093.0e-19148.18Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
Q9C7W45.7e-19048.01Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
M4ISH18.1e-17646.07Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DWM90.0e+0097.57long chain acyl-CoA synthetase 1 OS=Cucumis melo OX=3656 GN=LOC103489907 PE=4 SV... [more]
A0A0A0L8J20.0e+0098.03AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G535120... [more]
A0A5D3DAJ70.0e+0095.75Long chain acyl-CoA synthetase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7T9330.0e+0094.46Long chain acyl-CoA synthetase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1J2W00.0e+0090.27long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148218... [more]
Match NameE-valueIdentityDescription
XP_016900385.10.0e+0097.57PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo] >XP_016900386.1 PREDI... [more]
XP_011651533.10.0e+0098.03long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739343.1 long chain ac... [more]
TYK20490.10.0e+0095.75long chain acyl-CoA synthetase 1 [Cucumis melo var. makuwa][more]
KAA0038117.10.0e+0094.46long chain acyl-CoA synthetase 1 [Cucumis melo var. makuwa][more]
XP_038903045.10.0e+0091.88LOW QUALITY PROTEIN: long chain acyl-CoA synthetase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G47240.12.8e-27264.74AMP-dependent synthetase and ligase family protein [more]
AT2G47240.22.8e-27264.74AMP-dependent synthetase and ligase family protein [more]
AT4G23850.11.5e-19347.49AMP-dependent synthetase and ligase family protein [more]
AT4G11030.12.2e-19248.18AMP-dependent synthetase and ligase family protein [more]
AT1G64400.14.1e-19148.01AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 53..522
e-value: 1.2E-98
score: 330.6
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 28..514
e-value: 7.1E-83
score: 280.5
NoneNo IPR availablePANTHERPTHR43272:SF6LONG CHAIN ACYL-COA SYNTHETASE 1coord: 1..656
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 1..656
NoneNo IPR availableCDDcd05927LC-FACS_eukcoord: 72..653
e-value: 0.0
score: 739.414
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 40..651
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 225..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0020761.1PI0020761.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010143 cutin biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
biological_process GO:0010025 wax biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity