PI0020627 (gene) Melon (PI 482460) v1

Overview
NamePI0020627
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptiondisease resistance protein RGA2-like
Locationchr04: 7938063 .. 7939313 (-)
RNA-Seq ExpressionPI0020627
SyntenyPI0020627
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGATCTAATGCATGAAATTGCTTGTGCAATTTCAAGTCATCAAAAGATAGAATCAAATTCAAATAATTTGAGTCGAAAAAGTGCATTGCGCACATTGATTTTCAATGATAAAAATATTGATTATAAGGTTTTGGACGACCTTGTTTGTTTGCGTGTTTTAAAATTGAATTGGCATTATTGGGCTAATGATTTCCGGATTCCTCTGAACAAGTTGATACATTTAAGATATCTCGACATTTCAGATCGTTCAGCTGGATACAAGTTTGCTCCAAAATTTGATAGAATTTCTGCACTTTATAATTTACAAACGCTAAAGCTTGGACGCATTCAAAGTGGTGATCTACCAAAGAATTTGAAAAATTTGGTTAATCTAAGACATTTAGAATTCGACACACATCATATGAAACAAATGCCTTCGAATATGGGCAACTTGATTCATCTTCAAACATTGCCCAAGTTTGTAATTGGGCTTGAGAAGGGTTGTAAAATAGAAGAGCTTGGGCCGTTGAAAAACCTCAAAGGTAAACTAACTCTTAATCATCTTGGGAATGTGCAAAGTAAAGAGGAAGCTATGGCTGCAAAATTGAGGGAAAAGAAGAATTTACGTCATCTAGATCTAATCTTTGAGGGGTTTGCTTGGGGAAGAATTGATAATTACGATGACCAGATACAAGTGTTGGAAGAACTTCAACCAAACAACAATCTACAAGTATTGGAAATTAGGTGTTTTCAAGGGAGATATTTGCCTAGTATTTTTGTTGAAAATTTAGTTGAGATACATTTGTGTGATTGTGAAAGATGTGAAGAGCTTCCAATGCTTGGACAGTTGCCCAACTTAAAGATACTTGTGATTACGAGCATGAATAGTGTGAGAAGTATAGGGAATGAGTTCTATGGAATTAACTCCAATGACCACACAAATTCTTTTGCTTTTCCCAAGTTAGCGGAACTTCATATTTATGAGATGCAGAAGCTAGAGCAATGGGATGAAGCAACGGTTGTTATTGCATCAAATCTCTTTGGATGTCTAAAAAAGCTTTGTATTCATGGATGCAGTCAATTGGCAAAATTGCCAAGTGGGTTGGAAGGTTGCCAATCCATTGAGTATTTGAGGATAAATAATTGTTCTAATTTGATGCTAAATGTGAAAAATTTGCACAACTTACATTCTTTAGAGATTCCTGGGTTGAAAAGATTGCCAGAGGGATTAGGTGAGCTCACTCACTTGAAAGAATTGATAATTTGA

mRNA sequence

ATGCATGATCTAATGCATGAAATTGCTTGTGCAATTTCAAGTCATCAAAAGATAGAATCAAATTCAAATAATTTGAGTCGAAAAAGTGCATTGCGCACATTGATTTTCAATGATAAAAATATTGATTATAAGGTTTTGGACGACCTTGTTTGTTTGCGTGTTTTAAAATTGAATTGGCATTATTGGGCTAATGATTTCCGGATTCCTCTGAACAAGTTGATACATTTAAGATATCTCGACATTTCAGATCGTTCAGCTGGATACAAGTTTGCTCCAAAATTTGATAGAATTTCTGCACTTTATAATTTACAAACGCTAAAGCTTGGACGCATTCAAAGTGGTGATCTACCAAAGAATTTGAAAAATTTGGTTAATCTAAGACATTTAGAATTCGACACACATCATATGAAACAAATGCCTTCGAATATGGGCAACTTGATTCATCTTCAAACATTGCCCAAGTTTGTAATTGGGCTTGAGAAGGGTTGTAAAATAGAAGAGCTTGGGCCGTTGAAAAACCTCAAAGGTAAACTAACTCTTAATCATCTTGGGAATGTGCAAAGTAAAGAGGAAGCTATGGCTGCAAAATTGAGGGAAAAGAAGAATTTACGTCATCTAGATCTAATCTTTGAGGGGTTTGCTTGGGGAAGAATTGATAATTACGATGACCAGATACAAGTGTTGGAAGAACTTCAACCAAACAACAATCTACAAGTATTGGAAATTAGGTGTTTTCAAGGGAGATATTTGCCTAGTATTTTTGTTGAAAATTTAGTTGAGATACATTTGTGTGATTGTGAAAGATGTGAAGAGCTTCCAATGCTTGGACAGTTGCCCAACTTAAAGATACTTGTGATTACGAGCATGAATAGTGTGAGAAGTATAGGGAATGAGTTCTATGGAATTAACTCCAATGACCACACAAATTCTTTTGCTTTTCCCAAGTTAGCGGAACTTCATATTTATGAGATGCAGAAGCTAGAGCAATGGGATGAAGCAACGGTTGTTATTGCATCAAATCTCTTTGGATGTCTAAAAAAGCTTTGTATTCATGGATGCAGTCAATTGGCAAAATTGCCAAGTGGGTTGGAAGGTTGCCAATCCATTGAGTATTTGAGGATAAATAATTGTTCTAATTTGATGCTAAATGTGAAAAATTTGCACAACTTACATTCTTTAGAGATTCCTGGGTTGAAAAGATTGCCAGAGGGATTAGGTGAGCTCACTCACTTGAAAGAATTGATAATTTGA

Coding sequence (CDS)

ATGCATGATCTAATGCATGAAATTGCTTGTGCAATTTCAAGTCATCAAAAGATAGAATCAAATTCAAATAATTTGAGTCGAAAAAGTGCATTGCGCACATTGATTTTCAATGATAAAAATATTGATTATAAGGTTTTGGACGACCTTGTTTGTTTGCGTGTTTTAAAATTGAATTGGCATTATTGGGCTAATGATTTCCGGATTCCTCTGAACAAGTTGATACATTTAAGATATCTCGACATTTCAGATCGTTCAGCTGGATACAAGTTTGCTCCAAAATTTGATAGAATTTCTGCACTTTATAATTTACAAACGCTAAAGCTTGGACGCATTCAAAGTGGTGATCTACCAAAGAATTTGAAAAATTTGGTTAATCTAAGACATTTAGAATTCGACACACATCATATGAAACAAATGCCTTCGAATATGGGCAACTTGATTCATCTTCAAACATTGCCCAAGTTTGTAATTGGGCTTGAGAAGGGTTGTAAAATAGAAGAGCTTGGGCCGTTGAAAAACCTCAAAGGTAAACTAACTCTTAATCATCTTGGGAATGTGCAAAGTAAAGAGGAAGCTATGGCTGCAAAATTGAGGGAAAAGAAGAATTTACGTCATCTAGATCTAATCTTTGAGGGGTTTGCTTGGGGAAGAATTGATAATTACGATGACCAGATACAAGTGTTGGAAGAACTTCAACCAAACAACAATCTACAAGTATTGGAAATTAGGTGTTTTCAAGGGAGATATTTGCCTAGTATTTTTGTTGAAAATTTAGTTGAGATACATTTGTGTGATTGTGAAAGATGTGAAGAGCTTCCAATGCTTGGACAGTTGCCCAACTTAAAGATACTTGTGATTACGAGCATGAATAGTGTGAGAAGTATAGGGAATGAGTTCTATGGAATTAACTCCAATGACCACACAAATTCTTTTGCTTTTCCCAAGTTAGCGGAACTTCATATTTATGAGATGCAGAAGCTAGAGCAATGGGATGAAGCAACGGTTGTTATTGCATCAAATCTCTTTGGATGTCTAAAAAAGCTTTGTATTCATGGATGCAGTCAATTGGCAAAATTGCCAAGTGGGTTGGAAGGTTGCCAATCCATTGAGTATTTGAGGATAAATAATTGTTCTAATTTGATGCTAAATGTGAAAAATTTGCACAACTTACATTCTTTAGAGATTCCTGGGTTGAAAAGATTGCCAGAGGGATTAGGTGAGCTCACTCACTTGAAAGAATTGATAATTTGA

Protein sequence

MHDLMHEIACAISSHQKIESNSNNLSRKSALRTLIFNDKNIDYKVLDDLVCLRVLKLNWHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNNNLQVLEIRCFQGRYLPSIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKELII
Homology
BLAST of PI0020627 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.0e-35
Identity = 120/358 (33.52%), Postives = 180/358 (50.28%), Query Frame = 0

Query: 38  DKNIDYKVLDDLVCLRVLKLNWHYWANDFRIP---LNKLIHLRYLDISDRSAGYKFAPKF 97
           D+ +  K+L  L  LRVL L+ HY     R+P      + H R+LD+S      +  PK 
Sbjct: 566 DQMVSEKLLPTLTRLRVLSLS-HY--KIARLPPDFFKNISHARFLDLS--RTELEKLPK- 625

Query: 98  DRISALYNLQTLKLGRIQS-GDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLP 157
             +  +YNLQTL L    S  +LP ++ NL+NLR+L+     ++QMP   G L  LQTL 
Sbjct: 626 -SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLT 685

Query: 158 KFVIGLEKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGF 217
            F +    G +I ELG L +L GKL +  L  V    +A  A L  KK+LR +D ++   
Sbjct: 686 TFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTG 745

Query: 218 AWGRIDNYD-----DQIQVLEELQPNNNLQVLEIRCFQGRYLPSIFVE----NLVEIHLC 277
           +    +N +     ++ +V E+L+P+ +++ L I  ++GR  P    +     +V I L 
Sbjct: 746 SSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLR 805

Query: 278 DCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSN-DHTNSFAFPKLAELHIYE 337
           +C+ C  LP LGQLP LK L I+ M  ++SIG +FY  +      +   F  L  L    
Sbjct: 806 ECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDN 865

Query: 338 MQKLEQWDEATVVIASNLFGCLKKLCIHGCSQL-AKLPSGLEGCQSIEYLRINNCSNL 381
           +   ++W +  V    +LF  LKKL I  C +L   LP+ L    S+  L I  C  L
Sbjct: 866 LPDWQEWLDVRVT-RGDLFPSLKKLFILRCPELTGTLPTFL---PSLISLHIYKCGLL 912

BLAST of PI0020627 vs. ExPASy Swiss-Prot
Match: Q9LRR5 (Putative disease resistance protein At3g14460 OS=Arabidopsis thaliana OX=3702 GN=At3g14460 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.1e-32
Identity = 115/346 (33.24%), Postives = 170/346 (49.13%), Query Frame = 0

Query: 29  SALRTLIFNDKNIDYKVLDDLVCLRVLKLNWHYWANDFRIPLNKLIHLRYLDISDRSAGY 88
           ++L +L   +K ++  +L+ L  LR+L L+ HY   +    L  L  LRYLD+S  S   
Sbjct: 553 TSLESLQLTEKVLN-PLLNALSGLRILSLS-HYQITNLPKSLKGLKLLRYLDLS--STKI 612

Query: 89  KFAPKFDRISALYNLQTLKLGRIQS-GDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLI 148
           K  P+F  +  L NLQTL L   +    LPK++  L+NLR L+     + +MP  +  L 
Sbjct: 613 KELPEF--VCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLR 672

Query: 149 HLQTLPKFVIGLEKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLD 208
            LQ L  FVIG   G  + EL  L +L+G L ++ L NV    EA  A L+ K  L  L 
Sbjct: 673 SLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLI 732

Query: 209 LIF----EGFAWGRIDNYD-DQIQVLEELQPNNNLQVLEIRCFQGRYLPSIFVEN----L 268
           L +     GF  G  +    DQ +VL  L+P+ +L+   I  +QG   P    ++    +
Sbjct: 733 LKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGI 792

Query: 269 VEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSNDHTNSFAFPKLAE 328
             + L  C  C  LP +GQLP+LK L I   N ++ +G +F+   +N  +    F  L  
Sbjct: 793 TSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENN--SRGVPFQSLQI 852

Query: 329 LHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQL-AKLPSGL 364
           L  Y M + ++W      +   +F CL+KL I  C  L  K P GL
Sbjct: 853 LKFYGMPRWDEW--ICPELEDGIFPCLQKLIIQRCPSLRKKFPEGL 888

BLAST of PI0020627 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.1e-32
Identity = 137/436 (31.42%), Postives = 210/436 (48.17%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSALRTLIFNDKNIDY----KVLDDLVCLRVLK 60
           MHDL+H++A ++ S     SN   +++ S    +      + +      L+  + LRVL 
Sbjct: 473 MHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEVVFFYTLPPLEKFISLRVLN 532

Query: 61  LNWHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGR-IQSGD 120
           L    + N     +  L+HLRYL++    +G +  PK  ++  L NLQTL L    +   
Sbjct: 533 LGDSTF-NKLPSSIGDLVHLRYLNL--YGSGMRSLPK--QLCKLQNLQTLDLQYCTKLCC 592

Query: 121 LPKNLKNLVNLRHLEFD-THHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNL 180
           LPK    L +LR+L  D +  +  MP  +G+L  L+TL +FV+G +KG ++ ELG L NL
Sbjct: 593 LPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NL 652

Query: 181 KGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPN 240
            G + ++HL  V++ ++A  A L  K NL  L + +  F  G      ++++VLE L+P+
Sbjct: 653 YGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNF--GPHIYESEEVKVLEALKPH 712

Query: 241 NNLQVLEIRCFQGRYLPS----IFVENLVEIHLCDCERCEELPMLGQLPNLKILVI-TSM 300
           +NL  L+I  F+G +LP       ++N+V I + +   C  LP  G LP L+ L +    
Sbjct: 713 SNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGS 772

Query: 301 NSVRSIGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLC 360
             V  +      ++S   T    FP L +L I++   L+   +         F  L+++ 
Sbjct: 773 ADVEYVEEVDIDVHSGFPTR-IRFPSLRKLDIWDFGSLKGLLKKE---GEEQFPVLEEMI 832

Query: 361 IHGCSQLAKLPSGLEGCQSIEYLRINNCSNLMLN------VKNLHNLHSLEIP---GLKR 417
           IH C  L  L S L    S   LRI  C N +         KNL NL  L I     LK 
Sbjct: 833 IHECPFLT-LSSNLRALTS---LRI--CYNKVATSFPEEMFKNLANLKYLTISRCNNLKE 890

BLAST of PI0020627 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 131.0 bits (328), Expect = 3.2e-29
Identity = 134/445 (30.11%), Postives = 199/445 (44.72%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSN--NLSRKSALRTLIFNDKNIDY--KVLDDLVCLRVLK 60
           MHDL+H++A ++ S     SN    N +    + ++ F +    Y   +L   V LRVL 
Sbjct: 472 MHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYSPSLLQKFVSLRVLN 531

Query: 61  LNWHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQS-GD 120
           L  +   N     +  L+HLRYLD+   S  ++      R+  L NLQTL L    S   
Sbjct: 532 LR-NSNLNQLPSSIGDLVHLRYLDL---SGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSC 591

Query: 121 LPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLK 180
           LPK    L +LR+L  D   +   P  +G L  L++L  FVIG  KG ++ EL  L NL 
Sbjct: 592 LPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLY 651

Query: 181 GKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNN 240
           G +++  L  V+   +A  A L  K NL  L L     +W     +    +VLE L+P++
Sbjct: 652 GSISITKLDRVKKDTDAKEANLSAKANLHSLCL-----SWDLDGKHRYDSEVLEALKPHS 711

Query: 241 NLQVLEIRCFQGRYLP----SIFVENLVEIHLCDCERCEELPMLGQLPNLKILVI-TSMN 300
           NL+ LEI  F G  LP       ++N+V I +  CE C  LP  G+LP L+ L + T   
Sbjct: 712 NLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELHTGSA 771

Query: 301 SVRSIGNEFYGINSNDHTNSFAFPKLAELHIYE------MQKLEQWDEATVVIASNLFGC 360
            V  +          D+ +   FP L +L I++      + K+E   +  V+     + C
Sbjct: 772 DVEYV---------EDNVHPGRFPSLRKLVIWDFSNLKGLLKMEGEKQFPVLEEMTFYWC 831

Query: 361 LKKL--CIHGCSQLAKLPSGLEGCQSIEYLRINNCSNLMLNV----------KNLHNLHS 415
              +   +     L  + +     +SI  LR     ++  NV          K+L NL  
Sbjct: 832 PMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPEEMFKSLANLKY 891

BLAST of PI0020627 vs. ExPASy Swiss-Prot
Match: Q8RXS5 (Probable disease resistance protein At5g63020 OS=Arabidopsis thaliana OX=3702 GN=At5g63020 PE=2 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 3.0e-11
Identity = 76/282 (26.95%), Postives = 121/282 (42.91%), Query Frame = 0

Query: 95  DRISALYNLQTLKLGRIQSGDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPK 154
           + IS   +LQ L L R +    P  L  L  L +L  +   M +    +  L  L+ L  
Sbjct: 575 NEISECVSLQYLSLSRTRIRIWPAGLVELRKLLYLNLEYTRMVESICGISGLTSLKVLRL 634

Query: 155 FVIGL-EKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGF 214
           FV G  E  C + EL  L+NL+   TL     + S  E   +  R     R L       
Sbjct: 635 FVSGFPEDPCVLNELQLLENLQ---TLTITLGLASILEQFLSNQRLASCTRAL------- 694

Query: 215 AWGRIDNYDDQIQVLEELQPNNNLQVL--------EIRCFQGR-----YLP--SIFVENL 274
              RI+N + Q  V+  +   ++LQ L        EI+  +       ++P  + F  NL
Sbjct: 695 ---RIENLNPQSSVISFVATMDSLQELHFADSDIWEIKVKRNETVLPLHIPTTTTFFPNL 754

Query: 275 VEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSNDHTNSFAFPKLAE 334
            ++ L  C R  +L  L   PNL +L + S + ++ + N+       +  N   F +L E
Sbjct: 755 SQVSLEFCTRLRDLTWLIFAPNLTVLRVISASDLKEVINK----EKAEQQNLIPFQELKE 814

Query: 335 LHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLAKLP 361
           L +  +Q L+      +      F CL+K+ ++GCS+L KLP
Sbjct: 815 LRLENVQMLKHIHRGPLP-----FPCLQKILVNGCSELRKLP 834

BLAST of PI0020627 vs. ExPASy TrEMBL
Match: A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 5.0e-134
Identity = 274/419 (65.39%), Postives = 317/419 (75.66%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           MHDLMH+IACAISSHQ +ESN NNLS KS   LRTLI ND+ I+Y   +D+VCLRVLK+ 
Sbjct: 506 MHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRTLICNDEVINYLNQNDIVCLRVLKVI 565

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
           +     D  IP++KLIHLRYLDIS+ S         + +S LYNLQTLKLG  QSG LPK
Sbjct: 566 FQS-HTDLWIPIDKLIHLRYLDISECSINKLL---LESLSLLYNLQTLKLG--QSG-LPK 625

Query: 121 NLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGKL 180
           NL+ LVNLRHLEF       MPS+MGNLIHLQ+L  F++G EKGCKIEELGPLKNLKGKL
Sbjct: 626 NLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKL 685

Query: 181 TLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNNNLQ 240
           TL +L  VQ+K+EAMAAKL EKKNLRHL+L F        D+ D  +QVLE LQP+ NLQ
Sbjct: 686 TLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDDEDGIVQVLEGLQPHKNLQ 745

Query: 241 VLEIRCFQGRYLPS-IFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGN 300
            LEI  F+G+ LP+ IFVENLV+I L   ERCE LPMLGQLPNLK L I  M SVRSIGN
Sbjct: 746 SLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGN 805

Query: 301 EFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLA 360
           EFYG++S+ H NS AFP+L +L IYEM  LEQWDEATVV+ SNLFGCLK++ I  C+ LA
Sbjct: 806 EFYGVDSS-HQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLA 865

Query: 361 KLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKELII 417
           KLPSGLEGC S+EYL I  C NLMLNV+NLH L+ LEI GLKRLP+G+  LT LKEL I
Sbjct: 866 KLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKRLPKGMDGLTRLKELKI 916

BLAST of PI0020627 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 4.8e-129
Identity = 267/424 (62.97%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           +HDLMH+IACAIS+H K++SN  + + KS   LRTLI  ++   +K+  D++CLRVL L 
Sbjct: 497 IHDLMHDIACAISNHHKMDSNPISWNGKSTRKLRTLICENEEAFHKIQTDIICLRVLVLK 556

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
           W +  N     ++KLIHLRYLDIS+ +         D I ALYNLQTLKLG I+  DLPK
Sbjct: 557 W-FDTNTLSTIMDKLIHLRYLDISNCNINKLLR---DSICALYNLQTLKLGYIEC-DLPK 616

Query: 121 NLKNLVNLRHLEFDT-HHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGK 180
           NL+NLVNLRHLEF     M QMPS+MGN+IHLQTL +FV+GLEKGCKI+ELGPLK+LKG 
Sbjct: 617 NLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGLEKGCKIDELGPLKDLKGT 676

Query: 181 LTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFA----WGRIDNYDDQIQVLEELQP 240
           LTL +L NVQ+K+EAMAAKL EKK LRH  LIF+ F      G  D  DD  QVLE LQP
Sbjct: 677 LTLKNLQNVQNKDEAMAAKLVEKKYLRH--LIFQWFLNLYDRGEYDE-DDNKQVLEGLQP 736

Query: 241 NNNLQVLEIRCFQGRYL-PSIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSV 300
           + N+Q L+IR FQGR L  +IFVENLVEI L DC RCE LPMLGQLPNLK L I SMNSV
Sbjct: 737 HKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPMLGQLPNLKKLEIISMNSV 796

Query: 301 RSIGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHG 360
           RSIG+EFYG++ ND  NS AFP+L + HI  ++KL+QWDEAT V ASN FGCLK+L + G
Sbjct: 797 RSIGSEFYGVDCNDR-NSSAFPQLNKFHICGLKKLQQWDEAT-VFASNRFGCLKELILSG 856

Query: 361 CSQLAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLK 417
           C QLAKLPSGLEGC SIEYL I+ C NLMLNV+NL+NL+ L+I GLKRLP+  G+LT+LK
Sbjct: 857 CHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLPDEFGKLTNLK 910

BLAST of PI0020627 vs. ExPASy TrEMBL
Match: A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 1.1e-96
Identity = 224/420 (53.33%), Postives = 276/420 (65.71%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQK-IESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKL 60
           MHDL+H+IACAIS+H K +E  S+N SRKSA  LRTLI N++ I Y  L D V LRVL L
Sbjct: 496 MHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLICNNQVI-YNELMDCVSLRVLVL 555

Query: 61  NWHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLP 120
           N     ++    + KL HLRYLDIS     Y      + I+ LYNLQTLK+GR++S  LP
Sbjct: 556 N-SAKTDNLLDSIGKLKHLRYLDISH----YSIRVLPESIAKLYNLQTLKIGRMES--LP 615

Query: 121 KNLKNLVNLRHLEF-DTHHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKG 180
           KNL+ +V+LRHL+F   +H  QMPS+M +LIHLQTL +F  G E GCKIEELGPLKNLKG
Sbjct: 616 KNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAGFENGCKIEELGPLKNLKG 675

Query: 181 KLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWG-RIDNYDDQIQVLEELQPNN 240
           +L L++L  V+SKEEA+AAKL EKKNL  L      F W    +  DD  +VLE LQP+ 
Sbjct: 676 RLRLSNLERVKSKEEAIAAKLVEKKNLHELT-----FDWSLDFERNDDDSEVLEGLQPHK 735

Query: 241 NLQVLEIRCFQGRYLP-SIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRS 300
           NLQ L I  F G+ LP +IFVENL  I LC  ++CE LPMLGQLPNL  L I+ M+SVR 
Sbjct: 736 NLQSLNIMNFGGQVLPEAIFVENLAVIRLCGGKKCEMLPMLGQLPNLVELEISFMDSVRC 795

Query: 301 IGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCS 360
           IG+EFYG  S        F KL +     +  LEQW EA  +  SN FG L+ L + GC 
Sbjct: 796 IGSEFYGNGS--------FSKLRKFDFIGLHNLEQWTEAASI--SNSFGSLQTLNVIGCG 855

Query: 361 QLAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKEL 415
            L KLP+GLE C S+  L + +C  L LNV+N+HNL +L I G K LP GL  LT+LKE+
Sbjct: 856 NLKKLPNGLEFCSSLHDLTVRDCPKLALNVQNMHNLSNLFIHGFKELPLGLSHLTNLKEM 892

BLAST of PI0020627 vs. ExPASy TrEMBL
Match: A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 1.9e-96
Identity = 223/420 (53.10%), Postives = 281/420 (66.90%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKI--ESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLK 60
           MHDL+H+IACAIS+HQK+  E +S+N SRKSA  LRTLI N++ I Y  L D V LRVL 
Sbjct: 498 MHDLIHDIACAISNHQKLLHELSSSNRSRKSARKLRTLICNNQVI-YNELVDCVSLRVLV 557

Query: 61  LNWHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDL 120
           L+     N     + KL HLRYL I++   G       + I  LYNLQTLKL  ++S  L
Sbjct: 558 LHCEKTGNLLE-SIGKLKHLRYLSIAN---GNYVDVLPESIVMLYNLQTLKLRHMKS--L 617

Query: 121 PKNLKNLVNLRHLEFD-THHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLK 180
           PKN + +V+LRHLEF+     +QMPS+M  LIHLQTL +FV G E GCKIEELGPLKNLK
Sbjct: 618 PKNSRKMVSLRHLEFELKSDFRQMPSHMSELIHLQTLSRFVAGFENGCKIEELGPLKNLK 677

Query: 181 GKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNN 240
           GKL L+ L  V+SKEE++ AKL EKKNL+ L   +     G+   Y  +++VLE LQP+ 
Sbjct: 678 GKLQLSCLERVESKEESIVAKLVEKKNLQELIFEWSSRFEGKNSRY-SELEVLEGLQPHK 737

Query: 241 NLQVLEIRCFQGRYL-PSIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRS 300
           NLQ L +  F G+ L  +IFVENL  I L +C+ CE LPMLG+LPNL  L I SM+S+R 
Sbjct: 738 NLQSLTVIGFGGQVLGDAIFVENLTVIRLSNCKNCERLPMLGRLPNLVELNINSMDSLRC 797

Query: 301 IGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCS 360
           IG+EFYG  SN+  +SF+  KL +L     + LEQW +A VV  SN FG L+ L I+GC 
Sbjct: 798 IGSEFYGNGSNNQGHSFS--KLRKLTFIWCRNLEQWKDAAVV--SNSFGSLQTLSIYGCG 857

Query: 361 QLAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKEL 415
            L KLP+GLEGC S+  L +  C  L LNV+N  NL +L I GLK+LP GLG LT+LKE+
Sbjct: 858 NLEKLPNGLEGCSSLRDLTVAYCPKLSLNVQNCQNLSNLRIDGLKKLPLGLGHLTNLKEM 905

BLAST of PI0020627 vs. ExPASy TrEMBL
Match: A0A0A0KXG0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016360 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 8.4e-89
Identity = 210/421 (49.88%), Postives = 276/421 (65.56%), Query Frame = 0

Query: 1   MHDLMHEIACAI-SSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKL 60
           MHDL+H++A AI S+ QK   +  + + K++  LRTL++N++ I +KV  D V LRVL++
Sbjct: 408 MHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVA-DCVFLRVLEV 467

Query: 61  NWHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLP 120
           N  +  N+    + KL HLRYLDIS  S      P    ++ L+NLQTLKLG I+  +LP
Sbjct: 468 NSLHMMNNLPDFIAKLKHLRYLDIS--SCSMWVMP--HSVTTLFNLQTLKLGSIE--NLP 527

Query: 121 KNLKNLVNLRHLEFDTHH-MKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKG 180
            NL+NLV LRHLEF  ++  ++MPS+MG LIHLQ L  FV G E+GCKIEELG LKNLKG
Sbjct: 528 MNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKG 587

Query: 181 KLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNNN 240
           +L L++L  V+SKEEA+AAKL  KKNLR L   +         +Y+D  +VLE LQP  N
Sbjct: 588 QLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYND-FEVLEGLQPPKN 647

Query: 241 LQVLEIRCFQGRYLP-SIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSI 300
           L  L+I  F G++LP + FVENLV + L  C +CE LPMLGQL NL+ L I  M+SVRSI
Sbjct: 648 LSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSI 707

Query: 301 GNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQ 360
           G+EFYGI+SN       FPKL +     M  LEQW+       SN FG L+ L +  C +
Sbjct: 708 GSEFYGIDSN---RRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGK 767

Query: 361 LAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKELI 417
           L KLP+GLE C+S+  + I+NC NL LNV+ +HNL  L I GLK LP+GL    +LK ++
Sbjct: 768 LTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIM 817

BLAST of PI0020627 vs. NCBI nr
Match: XP_004136783.2 (disease resistance protein RGA2 [Cucumis sativus])

HSP 1 Score: 487.6 bits (1254), Expect = 1.0e-133
Identity = 274/419 (65.39%), Postives = 317/419 (75.66%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           MHDLMH+IACAISSHQ +ESN NNLS KS   LRTLI ND+ I+Y   +D+VCLRVLK+ 
Sbjct: 506 MHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRTLICNDEVINYLNQNDIVCLRVLKVI 565

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
           +     D  IP++KLIHLRYLDIS+ S         + +S LYNLQTLKLG  QSG LPK
Sbjct: 566 FQS-HTDLWIPIDKLIHLRYLDISECSINKLL---LESLSLLYNLQTLKLG--QSG-LPK 625

Query: 121 NLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGKL 180
           NL+ LVNLRHLEF       MPS+MGNLIHLQ+L  F++G EKGCKIEELGPLKNLKGKL
Sbjct: 626 NLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKL 685

Query: 181 TLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNNNLQ 240
           TL +L  VQ+K+EAMAAKL EKKNLRHL+L F        D+ D  +QVLE LQP+ NLQ
Sbjct: 686 TLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDDEDGIVQVLEGLQPHKNLQ 745

Query: 241 VLEIRCFQGRYLPS-IFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGN 300
            LEI  F+G+ LP+ IFVENLV+I L   ERCE LPMLGQLPNLK L I  M SVRSIGN
Sbjct: 746 SLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGN 805

Query: 301 EFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLA 360
           EFYG++S+ H NS AFP+L +L IYEM  LEQWDEATVV+ SNLFGCLK++ I  C+ LA
Sbjct: 806 EFYGVDSS-HQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLA 865

Query: 361 KLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKELII 417
           KLPSGLEGC S+EYL I  C NLMLNV+NLH L+ LEI GLKRLP+G+  LT LKEL I
Sbjct: 866 KLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKRLPKGMDGLTRLKELKI 916

BLAST of PI0020627 vs. NCBI nr
Match: KAE8651192.1 (hypothetical protein Csa_002145 [Cucumis sativus])

HSP 1 Score: 487.6 bits (1254), Expect = 1.0e-133
Identity = 274/419 (65.39%), Postives = 317/419 (75.66%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           MHDLMH+IACAISSHQ +ESN NNLS KS   LRTLI ND+ I+Y   +D+VCLRVLK+ 
Sbjct: 389 MHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRTLICNDEVINYLNQNDIVCLRVLKVI 448

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
           +     D  IP++KLIHLRYLDIS+ S         + +S LYNLQTLKLG  QSG LPK
Sbjct: 449 FQS-HTDLWIPIDKLIHLRYLDISECSINKLL---LESLSLLYNLQTLKLG--QSG-LPK 508

Query: 121 NLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGKL 180
           NL+ LVNLRHLEF       MPS+MGNLIHLQ+L  F++G EKGCKIEELGPLKNLKGKL
Sbjct: 509 NLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKL 568

Query: 181 TLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNNNLQ 240
           TL +L  VQ+K+EAMAAKL EKKNLRHL+L F        D+ D  +QVLE LQP+ NLQ
Sbjct: 569 TLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDDEDGIVQVLEGLQPHKNLQ 628

Query: 241 VLEIRCFQGRYLPS-IFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGN 300
            LEI  F+G+ LP+ IFVENLV+I L   ERCE LPMLGQLPNLK L I  M SVRSIGN
Sbjct: 629 SLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGN 688

Query: 301 EFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLA 360
           EFYG++S+ H NS AFP+L +L IYEM  LEQWDEATVV+ SNLFGCLK++ I  C+ LA
Sbjct: 689 EFYGVDSS-HQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLA 748

Query: 361 KLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKELII 417
           KLPSGLEGC S+EYL I  C NLMLNV+NLH L+ LEI GLKRLP+G+  LT LKEL I
Sbjct: 749 KLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKRLPKGMDGLTRLKELKI 799

BLAST of PI0020627 vs. NCBI nr
Match: XP_038877689.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 478.4 bits (1230), Expect = 6.3e-131
Identity = 270/421 (64.13%), Postives = 319/421 (75.77%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           MHDL+H+IAC +S+HQK  SN NN S KSA  LR LI N++   +K+  ++ CLRVL LN
Sbjct: 498 MHDLIHDIACMVSNHQKKASNPNNWSGKSARTLRALICNNEETPHKIQANINCLRVLVLN 557

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
             +  N+  I ++KLIHLRYLDIS  S         + +S+LYNLQTLK G I S DLPK
Sbjct: 558 -SFETNNLSIIMDKLIHLRYLDISKCSVNQL---SQESLSSLYNLQTLKFGYIGS-DLPK 617

Query: 121 NLKNLVNLRHLEFD-THHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGK 180
           NL+ LVNLRHLEF     MKQM S+MGNLIHLQTL +FV+GL K  KIEELGPLKNLKG 
Sbjct: 618 NLRKLVNLRHLEFQKKFDMKQMASHMGNLIHLQTLSEFVVGLGKAYKIEELGPLKNLKGT 677

Query: 181 LTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFAWGRIDNY-DDQIQVLEELQPNNN 240
           L + +L  VQ+KEEA+AAKL EKKNLRHL L +    + R + Y DD ++VLE LQP+ N
Sbjct: 678 LIITNLEKVQNKEEAIAAKLVEKKNLRHLILKWFVGLYNRGEYYEDDHVRVLEGLQPHKN 737

Query: 241 LQVLEIRCFQGRYLP-SIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSI 300
           LQ LEI  F+G+  P +IFV+NLVE+ L  CERC  LPMLGQLPNLK L+I SM SV+SI
Sbjct: 738 LQSLEINGFKGKVFPNNIFVQNLVEVDLYKCERCVMLPMLGQLPNLKKLLIRSMVSVKSI 797

Query: 301 GNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQ 360
           GNEFYGI+SN   NSFAFP+L ELHIY M+KLEQWDE+TVV +SNLFGCLKKL I GC Q
Sbjct: 798 GNEFYGIDSNQLRNSFAFPQLKELHIYGMKKLEQWDESTVV-SSNLFGCLKKLHISGCGQ 857

Query: 361 LAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLKELI 417
           LAKLPSGLEGC SIEYL I  CSNLMLNV+N +NL+ L+I GLKRLPEGLG+LT+LK L 
Sbjct: 858 LAKLPSGLEGCHSIEYLGIFQCSNLMLNVQNFYNLYHLDINGLKRLPEGLGKLTNLKILR 912

BLAST of PI0020627 vs. NCBI nr
Match: XP_031741283.1 (disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 471.1 bits (1211), Expect = 1.0e-128
Identity = 267/424 (62.97%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           +HDLMH+IACAIS+H K++SN  + + KS   LRTLI  ++   +K+  D++CLRVL L 
Sbjct: 380 IHDLMHDIACAISNHHKMDSNPISWNGKSTRKLRTLICENEEAFHKIQTDIICLRVLVLK 439

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
           W +  N     ++KLIHLRYLDIS+ +         D I ALYNLQTLKLG I+  DLPK
Sbjct: 440 W-FDTNTLSTIMDKLIHLRYLDISNCNINKLLR---DSICALYNLQTLKLGYIEC-DLPK 499

Query: 121 NLKNLVNLRHLEFDT-HHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGK 180
           NL+NLVNLRHLEF     M QMPS+MGN+IHLQTL +FV+GLEKGCKI+ELGPLK+LKG 
Sbjct: 500 NLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGLEKGCKIDELGPLKDLKGT 559

Query: 181 LTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFA----WGRIDNYDDQIQVLEELQP 240
           LTL +L NVQ+K+EAMAAKL EKK LRH  LIF+ F      G  D  DD  QVLE LQP
Sbjct: 560 LTLKNLQNVQNKDEAMAAKLVEKKYLRH--LIFQWFLNLYDRGEYDE-DDNKQVLEGLQP 619

Query: 241 NNNLQVLEIRCFQGRYL-PSIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSV 300
           + N+Q L+IR FQGR L  +IFVENLVEI L DC RCE LPMLGQLPNLK L I SMNSV
Sbjct: 620 HKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPMLGQLPNLKKLEIISMNSV 679

Query: 301 RSIGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHG 360
           RSIG+EFYG++ ND  NS AFP+L + HI  ++KL+QWDEAT V ASN FGCLK+L + G
Sbjct: 680 RSIGSEFYGVDCNDR-NSSAFPQLNKFHICGLKKLQQWDEAT-VFASNRFGCLKELILSG 739

Query: 361 CSQLAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLK 417
           C QLAKLPSGLEGC SIEYL I+ C NLMLNV+NL+NL+ L+I GLKRLP+  G+LT+LK
Sbjct: 740 CHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLPDEFGKLTNLK 793

BLAST of PI0020627 vs. NCBI nr
Match: XP_004140442.1 (disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical protein Csa_018865 [Cucumis sativus])

HSP 1 Score: 471.1 bits (1211), Expect = 1.0e-128
Identity = 267/424 (62.97%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 1   MHDLMHEIACAISSHQKIESNSNNLSRKSA--LRTLIFNDKNIDYKVLDDLVCLRVLKLN 60
           +HDLMH+IACAIS+H K++SN  + + KS   LRTLI  ++   +K+  D++CLRVL L 
Sbjct: 497 IHDLMHDIACAISNHHKMDSNPISWNGKSTRKLRTLICENEEAFHKIQTDIICLRVLVLK 556

Query: 61  WHYWANDFRIPLNKLIHLRYLDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPK 120
           W +  N     ++KLIHLRYLDIS+ +         D I ALYNLQTLKLG I+  DLPK
Sbjct: 557 W-FDTNTLSTIMDKLIHLRYLDISNCNINKLLR---DSICALYNLQTLKLGYIEC-DLPK 616

Query: 121 NLKNLVNLRHLEFDT-HHMKQMPSNMGNLIHLQTLPKFVIGLEKGCKIEELGPLKNLKGK 180
           NL+NLVNLRHLEF     M QMPS+MGN+IHLQTL +FV+GLEKGCKI+ELGPLK+LKG 
Sbjct: 617 NLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGLEKGCKIDELGPLKDLKGT 676

Query: 181 LTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGFA----WGRIDNYDDQIQVLEELQP 240
           LTL +L NVQ+K+EAMAAKL EKK LRH  LIF+ F      G  D  DD  QVLE LQP
Sbjct: 677 LTLKNLQNVQNKDEAMAAKLVEKKYLRH--LIFQWFLNLYDRGEYDE-DDNKQVLEGLQP 736

Query: 241 NNNLQVLEIRCFQGRYL-PSIFVENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSV 300
           + N+Q L+IR FQGR L  +IFVENLVEI L DC RCE LPMLGQLPNLK L I SMNSV
Sbjct: 737 HKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPMLGQLPNLKKLEIISMNSV 796

Query: 301 RSIGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHG 360
           RSIG+EFYG++ ND  NS AFP+L + HI  ++KL+QWDEAT V ASN FGCLK+L + G
Sbjct: 797 RSIGSEFYGVDCNDR-NSSAFPQLNKFHICGLKKLQQWDEAT-VFASNRFGCLKELILSG 856

Query: 361 CSQLAKLPSGLEGCQSIEYLRINNCSNLMLNVKNLHNLHSLEIPGLKRLPEGLGELTHLK 417
           C QLAKLPSGLEGC SIEYL I+ C NLMLNV+NL+NL+ L+I GLKRLP+  G+LT+LK
Sbjct: 857 CHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLPDEFGKLTNLK 910

BLAST of PI0020627 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 152.5 bits (384), Expect = 7.3e-37
Identity = 120/358 (33.52%), Postives = 180/358 (50.28%), Query Frame = 0

Query: 38  DKNIDYKVLDDLVCLRVLKLNWHYWANDFRIP---LNKLIHLRYLDISDRSAGYKFAPKF 97
           D+ +  K+L  L  LRVL L+ HY     R+P      + H R+LD+S      +  PK 
Sbjct: 566 DQMVSEKLLPTLTRLRVLSLS-HY--KIARLPPDFFKNISHARFLDLS--RTELEKLPK- 625

Query: 98  DRISALYNLQTLKLGRIQS-GDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLP 157
             +  +YNLQTL L    S  +LP ++ NL+NLR+L+     ++QMP   G L  LQTL 
Sbjct: 626 -SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLT 685

Query: 158 KFVIGLEKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGF 217
            F +    G +I ELG L +L GKL +  L  V    +A  A L  KK+LR +D ++   
Sbjct: 686 TFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTG 745

Query: 218 AWGRIDNYD-----DQIQVLEELQPNNNLQVLEIRCFQGRYLPSIFVE----NLVEIHLC 277
           +    +N +     ++ +V E+L+P+ +++ L I  ++GR  P    +     +V I L 
Sbjct: 746 SSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLR 805

Query: 278 DCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSN-DHTNSFAFPKLAELHIYE 337
           +C+ C  LP LGQLP LK L I+ M  ++SIG +FY  +      +   F  L  L    
Sbjct: 806 ECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDN 865

Query: 338 MQKLEQWDEATVVIASNLFGCLKKLCIHGCSQL-AKLPSGLEGCQSIEYLRINNCSNL 381
           +   ++W +  V    +LF  LKKL I  C +L   LP+ L    S+  L I  C  L
Sbjct: 866 LPDWQEWLDVRVT-RGDLFPSLKKLFILRCPELTGTLPTFL---PSLISLHIYKCGLL 912

BLAST of PI0020627 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 141.0 bits (354), Expect = 2.2e-33
Identity = 115/346 (33.24%), Postives = 170/346 (49.13%), Query Frame = 0

Query: 29  SALRTLIFNDKNIDYKVLDDLVCLRVLKLNWHYWANDFRIPLNKLIHLRYLDISDRSAGY 88
           ++L +L   +K ++  +L+ L  LR+L L+ HY   +    L  L  LRYLD+S  S   
Sbjct: 553 TSLESLQLTEKVLN-PLLNALSGLRILSLS-HYQITNLPKSLKGLKLLRYLDLS--STKI 612

Query: 89  KFAPKFDRISALYNLQTLKLGRIQS-GDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLI 148
           K  P+F  +  L NLQTL L   +    LPK++  L+NLR L+     + +MP  +  L 
Sbjct: 613 KELPEF--VCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLR 672

Query: 149 HLQTLPKFVIGLEKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLD 208
            LQ L  FVIG   G  + EL  L +L+G L ++ L NV    EA  A L+ K  L  L 
Sbjct: 673 SLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLI 732

Query: 209 LIF----EGFAWGRIDNYD-DQIQVLEELQPNNNLQVLEIRCFQGRYLPSIFVEN----L 268
           L +     GF  G  +    DQ +VL  L+P+ +L+   I  +QG   P    ++    +
Sbjct: 733 LKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGI 792

Query: 269 VEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSNDHTNSFAFPKLAE 328
             + L  C  C  LP +GQLP+LK L I   N ++ +G +F+   +N  +    F  L  
Sbjct: 793 TSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENN--SRGVPFQSLQI 852

Query: 329 LHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQL-AKLPSGL 364
           L  Y M + ++W      +   +F CL+KL I  C  L  K P GL
Sbjct: 853 LKFYGMPRWDEW--ICPELEDGIFPCLQKLIIQRCPSLRKKFPEGL 888

BLAST of PI0020627 vs. TAIR 10
Match: AT5G63020.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 71.2 bits (173), Expect = 2.1e-12
Identity = 76/282 (26.95%), Postives = 121/282 (42.91%), Query Frame = 0

Query: 95  DRISALYNLQTLKLGRIQSGDLPKNLKNLVNLRHLEFDTHHMKQMPSNMGNLIHLQTLPK 154
           + IS   +LQ L L R +    P  L  L  L +L  +   M +    +  L  L+ L  
Sbjct: 575 NEISECVSLQYLSLSRTRIRIWPAGLVELRKLLYLNLEYTRMVESICGISGLTSLKVLRL 634

Query: 155 FVIGL-EKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKLREKKNLRHLDLIFEGF 214
           FV G  E  C + EL  L+NL+   TL     + S  E   +  R     R L       
Sbjct: 635 FVSGFPEDPCVLNELQLLENLQ---TLTITLGLASILEQFLSNQRLASCTRAL------- 694

Query: 215 AWGRIDNYDDQIQVLEELQPNNNLQVL--------EIRCFQGR-----YLP--SIFVENL 274
              RI+N + Q  V+  +   ++LQ L        EI+  +       ++P  + F  NL
Sbjct: 695 ---RIENLNPQSSVISFVATMDSLQELHFADSDIWEIKVKRNETVLPLHIPTTTTFFPNL 754

Query: 275 VEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSNDHTNSFAFPKLAE 334
            ++ L  C R  +L  L   PNL +L + S + ++ + N+       +  N   F +L E
Sbjct: 755 SQVSLEFCTRLRDLTWLIFAPNLTVLRVISASDLKEVINK----EKAEQQNLIPFQELKE 814

Query: 335 LHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLAKLP 361
           L +  +Q L+      +      F CL+K+ ++GCS+L KLP
Sbjct: 815 LRLENVQMLKHIHRGPLP-----FPCLQKILVNGCSELRKLP 834

BLAST of PI0020627 vs. TAIR 10
Match: AT1G63350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 69.3 bits (168), Expect = 8.2e-12
Identity = 71/289 (24.57%), Postives = 123/289 (42.56%), Query Frame = 0

Query: 79  LDISDRSAGYKFAPKFDRISALYNLQTLKLGRIQSGDLPKNLKNLVNLRHLEFDTHHMKQ 138
           L + D S  Y  +   + IS L +LQ L L       LPK L+ L  L HL        +
Sbjct: 563 LAVLDLSGNYYLSELPNGISELVSLQYLNLSSTGIRHLPKGLQELKKLIHLYL------E 622

Query: 139 MPSNMGNLIHLQTLPKF-VIGLEKGCKIEELGPLKNLKGKLTLNHLGNVQSKEEAMAAKL 198
             S +G+++ +  L    V+ L       +L  +K L+    L HL  + +  +      
Sbjct: 623 RTSQLGSMVGISCLHNLKVLKLSGSSYAWDLDTVKELE---ALEHLEVLTTTIDDCTLGT 682

Query: 199 REKKNLRHLDLIFEGFAWGRIDNYDDQIQVLEELQPNNNLQVLEIRCFQGRYLPSIFV-- 258
            +  +   L        + +I N  ++ +    +     +  L+    +  +   I +  
Sbjct: 683 DQFLSSHRL---MSCIRFLKISNNSNRNRNSSRISLPVTMDRLQEFTIEHCHTSEIKMGR 742

Query: 259 ----ENLVEIHLCDCERCEELPMLGQLPNLKILVITSMNSVRSIGNEFYGINSNDHTNSF 318
                +L+E++L +C R  EL  L   PNLK L + S N +  I N+    +  + +   
Sbjct: 743 ICSFSSLIEVNLSNCRRLRELTFLMFAPNLKRLHVVSSNQLEDIINK-EKAHDGEKSGIV 802

Query: 319 AFPKLAELHIYEMQKLEQWDEATVVIASNLFGCLKKLCIHGCSQLAKLP 361
            FPKL ELH+Y +++L+      +  +   F CL+K+ + GC  L KLP
Sbjct: 803 PFPKLNELHLYNLRELK-----NIYWSPLPFPCLEKINVMGCPNLKKLP 833

BLAST of PI0020627 vs. TAIR 10
Match: AT5G44510.1 (target of AVRB operation1 )

HSP 1 Score: 63.5 bits (153), Expect = 4.5e-10
Identity = 58/202 (28.71%), Postives = 93/202 (46.04%), Query Frame = 0

Query: 225 IQVLEELQPNNNLQVLE-IRCFQGRYLPSIF--VENLVEIHLCDCERCEELP-MLGQLPN 284
           ++ L +     NLQ L  I C     LPS      NL+E+ L DC    +LP  +G L N
Sbjct: 670 LKELPDFSTATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPSSIGNLTN 729

Query: 285 LKILVITSMNSVRSIGNEFYGINSNDHTNSFAFPKLAELHIYEMQKLEQWDEATVVIASN 344
           LK L +   +S+  + + F  + S           L EL++     L +   +   I + 
Sbjct: 730 LKKLFLNRCSSLVKLPSSFGNVTS-----------LKELNLSGCSSLLEIPSSIGNIVN- 789

Query: 345 LFGCLKKLCIHGCSQLAKLPSGLEGCQSIEYLRINNCSNL------MLNVKNLHNLHSLE 404
               LKK+   GCS L +LPS +    +++ L + NCS+L      MLN+  L +L+   
Sbjct: 790 ----LKKVYADGCSSLVQLPSSIGNNTNLKELHLLNCSSLMECPSSMLNLTRLEDLNLSG 849

Query: 405 IPGLKRLPEGLGELTHLKELII 417
              L +LP  +G + +L+ L +
Sbjct: 850 CLSLVKLP-SIGNVINLQSLYL 854

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LRR41.0e-3533.52Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Q9LRR53.1e-3233.24Putative disease resistance protein At3g14460 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q7XBQ93.1e-3231.42Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA423.2e-2930.11Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q8RXS53.0e-1126.95Probable disease resistance protein At5g63020 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LBV05.0e-13465.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1[more]
A0A0A0KST54.8e-12962.97Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1[more]
A0A6J1CVR61.1e-9653.33putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CU861.9e-9653.10disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A0A0KXG08.4e-8949.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016360 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004136783.21.0e-13365.39disease resistance protein RGA2 [Cucumis sativus][more]
KAE8651192.11.0e-13365.39hypothetical protein Csa_002145 [Cucumis sativus][more]
XP_038877689.16.3e-13164.13putative disease resistance protein RGA3 [Benincasa hispida][more]
XP_031741283.11.0e-12862.97disease resistance protein RGA2-like [Cucumis sativus][more]
XP_004140442.11.0e-12862.97disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical prote... [more]
Match NameE-valueIdentityDescription
AT3G14470.17.3e-3733.52NB-ARC domain-containing disease resistance protein [more]
AT3G14460.12.2e-3333.24LRR and NB-ARC domains-containing disease resistance protein [more]
AT5G63020.12.1e-1226.95Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G63350.18.2e-1224.57Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G44510.14.5e-1028.71target of AVRB operation1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 13..416
e-value: 1.6E-38
score: 134.0
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 1..380
NoneNo IPR availablePANTHERPTHR23155:SF1055OS05G0414200 PROTEINcoord: 1..380
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 1..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0020627.1PI0020627.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response