PI0020599 (gene) Melon (PI 482460) v1

BLAST of PI0020599 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 2.8e-233
Identity = 378/549 (68.85%), Postives = 469/549 (85.43%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           ME LKP PA+SSPLTP+GFLERA+ +Y D  S++Y++ +YTWSQT+ RCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           I  GHVVSV+APN P MYEL FAVPMAGAIL+ +NLRLDARTIS+ L HSESKL+F+D  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEFR 200
           S  LI +A+ALFP     P LV +AD++   +S +S E+G +F   SY++++ +GDP+F+
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADES---ESGNSSELGKEFFC-SYKDLIDRGDPDFK 180

Query: 201 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMFH 260
           W+ P SEWDP+++NYTSGTTSSPKGVVH HRGIF +T+DSLI+WGVPKQ VYLWTLPMFH
Sbjct: 181 WVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFH 240

Query: 261 ANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRPLE 320
           ANGWS+P+ +AA+GGTNIC+RKFD+ +I+ +I RH VTH+CGAPVVLNML+N+P + PL+
Sbjct: 241 ANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLK 300

Query: 321 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 380
             V+I+TAGAPPP+AVLFRTESLGF VSHGYGLTET GLV+ C+WK +WN LPATERARL
Sbjct: 301 TTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARL 360

Query: 381 KARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMSEE 440
           K+RQGV T+   + DV+D  +G +VK DG ++GE+VLRGGS+MLGY KDPE T K+M+ +
Sbjct: 361 KSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTAD 420

Query: 441 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 500
           GWF+TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPDE
Sbjct: 421 GWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDE 480

Query: 501 FWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 560
           FWGETPCAFVSL++GLT+ PTE+EI+EYCR KL  +MVPKTVVF E+LPKTSTGK+QKF+
Sbjct: 481 FWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFI 540

Query: 561 LREMAKSMG 570
           LR+MA+ MG
Sbjct: 541 LRDMARGMG 545

BLAST of PI0020599 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 2.1e-220
Identity = 374/556 (67.27%), Postives = 457/556 (82.19%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSS-I 80
           ME LKP  ANS PLTP+GFLERA+ +Y D  S++Y+  +YTWSQT+ RCLR+AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 81  GIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQ 140
           G+ KG VVSV+APN PAMYEL FAVPMAG +L+ IN RL+A TISV LRHSESKL+F+DQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 141 ASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPE- 200
            S  L+ DA++LFP +T  PLLVLIAD+   E+   +VE    FV   YE++V+KGD + 
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGEN--LTVEDREHFVC-CYEDLVEKGDDKT 180

Query: 201 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPM 260
           F+W+RP+SEWDPIV+NYTSGTTSSPKGVVHSHR +F VTLDSLI+WGVPKQ VYLWTLPM
Sbjct: 181 FKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPM 240

Query: 261 FHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRP 320
           FHANGW + + IAA+GGTNIC+R+FD  +IF+LI RH VTH+C AP+VLNML+NSPN  P
Sbjct: 241 FHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEP 300

Query: 321 LERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERA 380
           L  PV ++T GAPPPAAVL RTESLGF +SHGYG+TE  G+V+ C+WK +WN+LPATE+A
Sbjct: 301 LPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQA 360

Query: 381 RLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMS 440
           RLK+RQGVRT AM E DV+D  SG SVK DG+++GEIVL+G S+MLGY K+  AT K + 
Sbjct: 361 RLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIR 420

Query: 441 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 500
            +GWF+TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA P
Sbjct: 421 ADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACP 480

Query: 501 DEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQK 560
           DE+WGETP AFV+L++G+   PTE+EI+EYCR KLA FMVPK VVF ++LPKTSTGKIQK
Sbjct: 481 DEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQK 540

Query: 561 FLLREMAKSMGPSRKS 575
           F L+E+ K+MG S  S
Sbjct: 541 FTLKEIVKTMGYSSSS 553

BLAST of PI0020599 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 7.0e-216
Identity = 373/564 (66.13%), Postives = 456/564 (80.85%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNS-TTYTWSQTYLRCLRLASSLSSI 80
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S T YTW +T LRCLR+ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 81  GIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQ 140
           GI +  VVSV++PNTPAMYELQFAVPM+GAIL+ IN RLDART+SV LRH  SKLLF+D 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 141 ASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDF-VAD------SYEEMV 200
            S  L  +A+++    T  P+LV IAD           E GGD  VAD      +Y++++
Sbjct: 121 FSVDLAVEAISMMT--TDPPILVFIAD---------KEEEGGDADVADRTKFSYTYDDLI 180

Query: 201 QKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVY 260
            +GD +F+WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK  VY
Sbjct: 181 HRGDLDFKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVY 240

Query: 261 LWTLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTN 320
           LWTLP+FHANGWS+P+ IAA+GGTN+C+RKFDA LI+ LI  H VTH+CGAPVVLNML+ 
Sbjct: 241 LWTLPIFHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSA 300

Query: 321 SPNNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQL 380
           +   +PL RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTET GL + C+WK +WN+L
Sbjct: 301 TNEFQPLNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRL 360

Query: 381 PATERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEA 440
           PA++RARLKARQGVRT+   E DV+D ESG+SV+ +G ++GEIV+RG S+MLGY KDP  
Sbjct: 361 PASDRARLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVG 420

Query: 441 TKKAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEA 500
           T+KA+ + GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE 
Sbjct: 421 TEKAL-KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEV 480

Query: 501 AVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTS 560
           AVVARPD FWGETPCAFVSL+ GLT+ PTE E+IEYCR K+  +MVPKTV F+++LPKTS
Sbjct: 481 AVVARPDVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTS 540

Query: 561 TGKIQKFLLREMAKSMGPSRKSRL 577
           TGK+ KF+LRE+AK MG +R SR+
Sbjct: 541 TGKVMKFVLREIAKKMGTTRLSRM 552

BLAST of PI0020599 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 9.4e-213
Identity = 358/550 (65.09%), Postives = 447/550 (81.27%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNS-TTYTWSQTYLRCLRLASSLSSI 80
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S T YTW +T LRCLR+ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 81  GIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQ 140
           GI +  VVSV++PNTPAMYELQFAVPM+GAIL+ IN RLDART+SV LRH ESKLLF+D 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 141 ASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEF 200
            S  L  +A+++    T  P+LV+IAD       A   ++     + +Y++++++GDP F
Sbjct: 121 FSVDLAVEAVSMMT--TDPPILVVIADKEEEGGVADVADLSK--FSYTYDDLIERGDPGF 180

Query: 201 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMF 260
           +WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK  VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 261 HANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRPL 320
           H+NGW+ P+ IAA+GGTN+C+RKFDA LI+ LI  H VTH+CGAPVVLNML+ +  ++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 321 ERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERAR 380
             PV ILTAG+PPPA VL R ES+GF +SHGYGLTET G+++ C+WK KWN LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 381 LKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMSE 440
           LKARQGVRT+   E DV+D ESG SV+ +G ++GEIV+RG S+MLGY KDP  T+KA+ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420

Query: 441 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 500
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 501 EFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 560
           EFWGETPCAFVSL+ G +  PTEEE++EYCR K+  +MVPKTV FM++LPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 561 LLREMAKSMG 570
           +LR++AK MG
Sbjct: 541 VLRDIAKKMG 545

BLAST of PI0020599 vs. ExPASy Swiss-Prot
Match: O80658 (Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana OX=3702 GN=AEE4 PE=2 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 7.5e-202
Identity = 339/550 (61.64%), Postives = 439/550 (79.82%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLS--S 80
           ME L P  +NS PLT +GFLERA+ ++ DSPS+++ +T +TWS+T+ RCLR+AS+LS  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 81  IGIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFID 140
           +GI++G VVSVI PN P++YELQFAVPM+GA+L+ IN RLDA  +SV LRHSESKL+F+D
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 141 QASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPE 200
             SS+L+ +A++ F P  +RP LV++ D     D  SS     DF+ D+YE  +++GD  
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDG---NDMPSSSSADMDFL-DTYEGFMERGDLR 180

Query: 201 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPM 260
           F+W+RP SEW P+V+NYTSGTTSSPKGVVHSHR +F  T++SL++W +P + VYLWTLPM
Sbjct: 181 FKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPM 240

Query: 261 FHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRP 320
           FHANGWS+ +A AA+G  NIC+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +P
Sbjct: 241 FHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKP 300

Query: 321 LERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERA 380
           L+ PVK++TAGAPPPA V+ + E+LGFDVSHGYG+TETGGLV+ C+ K +W++L   ERA
Sbjct: 301 LQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERA 360

Query: 381 RLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMS 440
           + K+RQG+RT   AE DV D  SGKSVK DG ++GEIV RGGS+MLGY+KDPE T  +M 
Sbjct: 361 KQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMR 420

Query: 441 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 500
           E+GWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNPA+ EAAVVA+P
Sbjct: 421 EDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKP 480

Query: 501 DEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQK 560
           D+ WGETPCAFVSL+     V TE EI E+C+ KL  +MVP+ VVF+E+LPKTSTGKIQK
Sbjct: 481 DKMWGETPCAFVSLKYHDGSV-TEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQK 540

Query: 561 FLLREMAKSM 569
           FLLR+MAKS+
Sbjct: 541 FLLRQMAKSL 544

BLAST of PI0020599 vs. ExPASy TrEMBL
Match: A0A0A0KT33 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 8.6e-310
Identity = 537/573 (93.72%), Postives = 553/573 (96.51%), Query Frame = 0

Query: 4   EKQSNNHTPTTLSLSISMEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWS 63
           EKQ NNHT T+LSL ISMEHLKPTPANSSPLTPIGFLERAS+IYADSPSIIY+STT+TWS
Sbjct: 5   EKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWS 64

Query: 64  QTYLRCLRLASSLSSIGIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTI 123
           QTY RCLRLASSLSSIGIHKGHVVSVIAPNTP MYELQFAVPMAGAILSCINLRLDARTI
Sbjct: 65  QTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTI 124

Query: 124 SVQLRHSESKLLFIDQASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDF 183
           SVQLRHSESKLLF+DQASS LISDALALFPP  KRPLLVLI+DD AVEDSASSV IGGD 
Sbjct: 125 SVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGD- 184

Query: 184 VADSYEEMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIE 243
           V D YE+MVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIE
Sbjct: 185 VVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIE 244

Query: 244 WGVPKQSVYLWTLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGA 303
           WGVPK SVYLWTLPMFHANGWSFPFAIAA+GGTNICIRKFDAALIFSLI+RHHVTH+CGA
Sbjct: 245 WGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGA 304

Query: 304 PVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFC 363
           PVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVIFC
Sbjct: 305 PVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFC 364

Query: 364 SWKLKWNQLPATERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMM 423
           SWKL+WNQLPATERARLKARQGVRTLAMAEADVLDS +GK V+ DGVSIGEIV+RGGSMM
Sbjct: 365 SWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMM 424

Query: 424 LGYFKDPEATKKAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVL 483
           LGYFKDPEATKKAM+EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVL
Sbjct: 425 LGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVL 484

Query: 484 YTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVV 543
           YTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYC+GKLAGFMVPKTVV
Sbjct: 485 YTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVV 544

Query: 544 FMEQLPKTSTGKIQKFLLREMAKSMGPSRKSRL 577
           FMEQLPKTSTGKIQKFLLREMAKSMG S KSRL
Sbjct: 545 FMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576

BLAST of PI0020599 vs. ExPASy TrEMBL
Match: A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.8e-308
Identity = 529/556 (95.14%), Postives = 546/556 (98.20%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           M+HLKPTPANSSPLTPI FLERAS++YADSPSIIYNST++TWSQTYLRCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           IHKGHVVSVI+PNTP MYELQF+VPMAGAILSCINLRLDARTISVQLRHSESKLLF+DQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEFR 200
           SSALISDAL LFPP +KRPLLVLIADD AVEDSASSV IGGDFV D YE+M+QKGDPEFR
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFV-DFYEDMIQKGDPEFR 180

Query: 201 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMFH 260
           WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPK SVYLWTLPMFH
Sbjct: 181 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFH 240

Query: 261 ANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRPLE 320
           ANGWSFPFAIAA+GGTNICIRKFDAALIFSLI+RHHVTHLCGAPVVLNMLTNSPNNRPLE
Sbjct: 241 ANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLE 300

Query: 321 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 380
           RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL
Sbjct: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 360

Query: 381 KARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMSEE 440
           KARQGVRTLAMAEADVLDSE+GKSVK DGVSIGEIVLRGGSMMLGYFKDPEATKKAM+EE
Sbjct: 361 KARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEE 420

Query: 441 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 500
           GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480

Query: 501 FWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 560
           FWGETPCAFVSLREGL+RVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL
Sbjct: 481 FWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 540

Query: 561 LREMAKSMGPSRKSRL 577
           LREMAKSMGPSRKSRL
Sbjct: 541 LREMAKSMGPSRKSRL 555

BLAST of PI0020599 vs. ExPASy TrEMBL
Match: A0A1S3CIM6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501383 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.8e-308
Identity = 529/556 (95.14%), Postives = 546/556 (98.20%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           M+HLKPTPANSSPLTPI FLERAS++YADSPSIIYNST++TWSQTYLRCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           IHKGHVVSVI+PNTP MYELQF+VPMAGAILSCINLRLDARTISVQLRHSESKLLF+DQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEFR 200
           SSALISDAL LFPP +KRPLLVLIADD AVEDSASSV IGGDFV D YE+M+QKGDPEFR
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFV-DFYEDMIQKGDPEFR 180

Query: 201 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMFH 260
           WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPK SVYLWTLPMFH
Sbjct: 181 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFH 240

Query: 261 ANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRPLE 320
           ANGWSFPFAIAA+GGTNICIRKFDAALIFSLI+RHHVTHLCGAPVVLNMLTNSPNNRPLE
Sbjct: 241 ANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLE 300

Query: 321 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 380
           RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL
Sbjct: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 360

Query: 381 KARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMSEE 440
           KARQGVRTLAMAEADVLDSE+GKSVK DGVSIGEIVLRGGSMMLGYFKDPEATKKAM+EE
Sbjct: 361 KARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEE 420

Query: 441 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 500
           GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480

Query: 501 FWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 560
           FWGETPCAFVSLREGL+RVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL
Sbjct: 481 FWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 540

Query: 561 LREMAKSMGPSRKSRL 577
           LREMAKSMGPSRKSRL
Sbjct: 541 LREMAKSMGPSRKSRL 555

BLAST of PI0020599 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.2e-271
Identity = 472/558 (84.59%), Postives = 506/558 (90.68%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIYNSTTYTW++T+ RCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           I +G VVSV++PNT AMYEL FAVPMAGA+L+ INLRLDARTISV LRH E+ L+F+DQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVED--SASSVEIGGDFVADSYEEMVQKGDPE 200
           S  LISDALALFPP  KRP L+LIAD+ A ED  SASSV    DFV DSYEEMVQKGDP 
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFV-DSYEEMVQKGDPL 180

Query: 201 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPM 260
           F+WIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGVPK+SVYLWTLPM
Sbjct: 181 FQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPM 240

Query: 261 FHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRP 320
           FHANGWSFP+ IAA+GGTN+CIRKFDAA IF+LIHR+HVTHLCGAPVVLNMLTNSP+NRP
Sbjct: 241 FHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRP 300

Query: 321 LERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERA 380
           L+RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA ERA
Sbjct: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERA 360

Query: 381 RLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMS 440
           RLKARQGVRTLAMA ADVLD E+GKSVK DG SIGEIVLRG SMMLGY KDPEAT KAM+
Sbjct: 361 RLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMT 420

Query: 441 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 500
            EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP
Sbjct: 421 AEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480

Query: 501 DEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQK 560
           DEFWGETPCAFVSL+EGLTR PTE EIIEYCRGKL  FMVPKTVVF  +LPKTSTGKIQK
Sbjct: 481 DEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540

Query: 561 FLLREMAKSMGPSRKSRL 577
           F+LREMAKSMGP+ KSRL
Sbjct: 541 FVLREMAKSMGPAGKSRL 557

BLAST of PI0020599 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.7e-271
Identity = 472/562 (83.99%), Postives = 507/562 (90.21%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIYNSTTYTW++T+ RCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           I +G VVSV++PNTPAMYEL FAVPMAGA+L+ INLRLD+RTISV LRH E+ L+F+DQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVED------SASSVEIGGDFVADSYEEMVQK 200
           S  LISDALALFPP  KRP L+LIAD+ A ED      SASSV    DFV DSYEEMVQK
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFV-DSYEEMVQK 180

Query: 201 GDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLW 260
           GDP F+WIRPVSEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGV KQSVYLW
Sbjct: 181 GDPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLW 240

Query: 261 TLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSP 320
           TLPMFHANGWSFP+ IAA+GGTN+CIRKFDAA IF+LIHR+HVTHLCGAPVVLNMLTNSP
Sbjct: 241 TLPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSP 300

Query: 321 NNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPA 380
           +NRPL+RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA
Sbjct: 301 DNRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPA 360

Query: 381 TERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATK 440
            ERARLKARQGVRTLAMA ADVL  E+GKSVK DG SIGEIVLRG SMMLGY KDPEAT 
Sbjct: 361 AERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATA 420

Query: 441 KAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV 500
           KAM+ EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV
Sbjct: 421 KAMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV 480

Query: 501 VARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTG 560
           VARPDEFWGETPCAFVSL+EGLTR PTE EIIEYCRGKL  FMVPKTVVFM +LPKTSTG
Sbjct: 481 VARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTG 540

Query: 561 KIQKFLLREMAKSMGPSRKSRL 577
           KIQKF+LREMAKSMGP+ +SRL
Sbjct: 541 KIQKFVLREMAKSMGPAGRSRL 561

BLAST of PI0020599 vs. NCBI nr
Match: XP_004148909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50896.1 hypothetical protein Csa_023073 [Cucumis sativus])

HSP 1 Score: 1072.0 bits (2771), Expect = 1.8e-309
Identity = 537/573 (93.72%), Postives = 553/573 (96.51%), Query Frame = 0

Query: 4   EKQSNNHTPTTLSLSISMEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWS 63
           EKQ NNHT T+LSL ISMEHLKPTPANSSPLTPIGFLERAS+IYADSPSIIY+STT+TWS
Sbjct: 5   EKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWS 64

Query: 64  QTYLRCLRLASSLSSIGIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTI 123
           QTY RCLRLASSLSSIGIHKGHVVSVIAPNTP MYELQFAVPMAGAILSCINLRLDARTI
Sbjct: 65  QTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTI 124

Query: 124 SVQLRHSESKLLFIDQASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDF 183
           SVQLRHSESKLLF+DQASS LISDALALFPP  KRPLLVLI+DD AVEDSASSV IGGD 
Sbjct: 125 SVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGD- 184

Query: 184 VADSYEEMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIE 243
           V D YE+MVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIE
Sbjct: 185 VVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIE 244

Query: 244 WGVPKQSVYLWTLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGA 303
           WGVPK SVYLWTLPMFHANGWSFPFAIAA+GGTNICIRKFDAALIFSLI+RHHVTH+CGA
Sbjct: 245 WGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGA 304

Query: 304 PVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFC 363
           PVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVIFC
Sbjct: 305 PVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFC 364

Query: 364 SWKLKWNQLPATERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMM 423
           SWKL+WNQLPATERARLKARQGVRTLAMAEADVLDS +GK V+ DGVSIGEIV+RGGSMM
Sbjct: 365 SWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMM 424

Query: 424 LGYFKDPEATKKAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVL 483
           LGYFKDPEATKKAM+EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVL
Sbjct: 425 LGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVL 484

Query: 484 YTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVV 543
           YTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYC+GKLAGFMVPKTVV
Sbjct: 485 YTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVV 544

Query: 544 FMEQLPKTSTGKIQKFLLREMAKSMGPSRKSRL 577
           FMEQLPKTSTGKIQKFLLREMAKSMG S KSRL
Sbjct: 545 FMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576

BLAST of PI0020599 vs. NCBI nr
Match: XP_008463175.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046215.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18981.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.7e-308
Identity = 529/556 (95.14%), Postives = 546/556 (98.20%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           M+HLKPTPANSSPLTPI FLERAS++YADSPSIIYNST++TWSQTYLRCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           IHKGHVVSVI+PNTP MYELQF+VPMAGAILSCINLRLDARTISVQLRHSESKLLF+DQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEFR 200
           SSALISDAL LFPP +KRPLLVLIADD AVEDSASSV IGGDFV D YE+M+QKGDPEFR
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFV-DFYEDMIQKGDPEFR 180

Query: 201 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMFH 260
           WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPK SVYLWTLPMFH
Sbjct: 181 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFH 240

Query: 261 ANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRPLE 320
           ANGWSFPFAIAA+GGTNICIRKFDAALIFSLI+RHHVTHLCGAPVVLNMLTNSPNNRPLE
Sbjct: 241 ANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLE 300

Query: 321 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 380
           RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL
Sbjct: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARL 360

Query: 381 KARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMSEE 440
           KARQGVRTLAMAEADVLDSE+GKSVK DGVSIGEIVLRGGSMMLGYFKDPEATKKAM+EE
Sbjct: 361 KARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEE 420

Query: 441 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 500
           GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480

Query: 501 FWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 560
           FWGETPCAFVSLREGL+RVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL
Sbjct: 481 FWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 540

Query: 561 LREMAKSMGPSRKSRL 577
           LREMAKSMGPSRKSRL
Sbjct: 541 LREMAKSMGPSRKSRL 555

BLAST of PI0020599 vs. NCBI nr
Match: XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.9e-297
Identity = 516/578 (89.27%), Postives = 545/578 (94.29%), Query Frame = 0

Query: 2   QREKQSNNHTPTTLSLSISMEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYT 61
           +REKQSN++  TT+S SISME LKP+ ANSSPLTPIGFLERAS+IY DSPSIIYNSTTYT
Sbjct: 9   KREKQSNHNRATTISQSISMEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYT 68

Query: 62  WSQTYLRCLRLASSLSSIGIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDAR 121
           WS+TY RCLR+ASSLSSIGI KG+VVSVIAPNTP MYELQFAVPMAGA+L+CINLRLDAR
Sbjct: 69  WSETYRRCLRVASSLSSIGIQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDAR 128

Query: 122 TISVQLRHSESKLLFIDQASSALISDALALFPPHTKRPLLVLIADDTAVED---SASSVE 181
           TIS+ LRH ESKL+FIDQAS  LISDALALFPP TKRPLLVLIADDTAVED   S+SS  
Sbjct: 129 TISILLRHCESKLIFIDQASCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSV 188

Query: 182 IGGDFVADSYEEMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTL 241
           IGG+FV DSYEEMVQKGD EFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIF VTL
Sbjct: 189 IGGEFV-DSYEEMVQKGDDEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTL 248

Query: 242 DSLIEWGVPKQSVYLWTLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVT 301
           DSLIEWGVPKQSVYLWTLPMFHANGWSFPF IAA+GGTN+CIRKFDAALIFSLIHRHHVT
Sbjct: 249 DSLIEWGVPKQSVYLWTLPMFHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVT 308

Query: 302 HLCGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGG 361
           HLCGAPVVLNMLTNSP+N+PLERPVKILTAGAPPPAAVLFRTESLGFDV HGYGLTETGG
Sbjct: 309 HLCGAPVVLNMLTNSPDNQPLERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGG 368

Query: 362 LVIFCSWKLKWNQLPATERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLR 421
           LVI+CSWK KWN+LPATERARLKARQGVRTLAM+E DVLDSE+GKSVK DG SIGEIVLR
Sbjct: 369 LVIYCSWKSKWNRLPATERARLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLR 428

Query: 422 GGSMMLGYFKDPEATKKAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVE 481
           GGSMMLGYFKDPEATKKAM++EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVE
Sbjct: 429 GGSMMLGYFKDPEATKKAMTDEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVE 488

Query: 482 VESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMV 541
           VESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTR+PTEEEI+EYCR KL  FMV
Sbjct: 489 VESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMV 548

Query: 542 PKTVVFMEQLPKTSTGKIQKFLLREMAKSMGPSRKSRL 577
           PKTVVFME+LPKTSTGKIQKFLLRE AKSMGP+R SRL
Sbjct: 549 PKTVVFMEKLPKTSTGKIQKFLLREKAKSMGPARTSRL 585

BLAST of PI0020599 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 945.3 bits (2442), Expect = 2.5e-271
Identity = 472/558 (84.59%), Postives = 506/558 (90.68%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIYNSTTYTW++T+ RCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           I +G VVSV++PNT AMYEL FAVPMAGA+L+ INLRLDARTISV LRH E+ L+F+DQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVED--SASSVEIGGDFVADSYEEMVQKGDPE 200
           S  LISDALALFPP  KRP L+LIAD+ A ED  SASSV    DFV DSYEEMVQKGDP 
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFV-DSYEEMVQKGDPL 180

Query: 201 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPM 260
           F+WIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGVPK+SVYLWTLPM
Sbjct: 181 FQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPM 240

Query: 261 FHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRP 320
           FHANGWSFP+ IAA+GGTN+CIRKFDAA IF+LIHR+HVTHLCGAPVVLNMLTNSP+NRP
Sbjct: 241 FHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRP 300

Query: 321 LERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERA 380
           L+RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA ERA
Sbjct: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERA 360

Query: 381 RLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMS 440
           RLKARQGVRTLAMA ADVLD E+GKSVK DG SIGEIVLRG SMMLGY KDPEAT KAM+
Sbjct: 361 RLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMT 420

Query: 441 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 500
            EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP
Sbjct: 421 AEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480

Query: 501 DEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQK 560
           DEFWGETPCAFVSL+EGLTR PTE EIIEYCRGKL  FMVPKTVVF  +LPKTSTGKIQK
Sbjct: 481 DEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQK 540

Query: 561 FLLREMAKSMGPSRKSRL 577
           F+LREMAKSMGP+ KSRL
Sbjct: 541 FVLREMAKSMGPAGKSRL 557

BLAST of PI0020599 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 944.1 bits (2439), Expect = 5.6e-271
Identity = 472/562 (83.99%), Postives = 507/562 (90.21%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLSSIG 80
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIYNSTTYTW++T+ RCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 81  IHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQA 140
           I +G VVSV++PNTPAMYEL FAVPMAGA+L+ INLRLD+RTISV LRH E+ L+F+DQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 141 SSALISDALALFPPHTKRPLLVLIADDTAVED------SASSVEIGGDFVADSYEEMVQK 200
           S  LISDALALFPP  KRP L+LIAD+ A ED      SASSV    DFV DSYEEMVQK
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFV-DSYEEMVQK 180

Query: 201 GDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLW 260
           GDP F+WIRPVSEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGV KQSVYLW
Sbjct: 181 GDPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLW 240

Query: 261 TLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSP 320
           TLPMFHANGWSFP+ IAA+GGTN+CIRKFDAA IF+LIHR+HVTHLCGAPVVLNMLTNSP
Sbjct: 241 TLPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSP 300

Query: 321 NNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPA 380
           +NRPL+RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA
Sbjct: 301 DNRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPA 360

Query: 381 TERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATK 440
            ERARLKARQGVRTLAMA ADVL  E+GKSVK DG SIGEIVLRG SMMLGY KDPEAT 
Sbjct: 361 AERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATA 420

Query: 441 KAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV 500
           KAM+ EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV
Sbjct: 421 KAMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV 480

Query: 501 VARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTG 560
           VARPDEFWGETPCAFVSL+EGLTR PTE EIIEYCRGKL  FMVPKTVVFM +LPKTSTG
Sbjct: 481 VARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTG 540

Query: 561 KIQKFLLREMAKSMGPSRKSRL 577
           KIQKF+LREMAKSMGP+ +SRL
Sbjct: 541 KIQKFVLREMAKSMGPAGRSRL 561

BLAST of PI0020599 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 751.5 bits (1939), Expect = 4.9e-217
Identity = 373/564 (66.13%), Postives = 456/564 (80.85%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNS-TTYTWSQTYLRCLRLASSLSSI 80
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S T YTW +T LRCLR+ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 81  GIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQ 140
           GI +  VVSV++PNTPAMYELQFAVPM+GAIL+ IN RLDART+SV LRH  SKLLF+D 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 141 ASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDF-VAD------SYEEMV 200
            S  L  +A+++    T  P+LV IAD           E GGD  VAD      +Y++++
Sbjct: 121 FSVDLAVEAISMMT--TDPPILVFIAD---------KEEEGGDADVADRTKFSYTYDDLI 180

Query: 201 QKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVY 260
            +GD +F+WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK  VY
Sbjct: 181 HRGDLDFKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVY 240

Query: 261 LWTLPMFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTN 320
           LWTLP+FHANGWS+P+ IAA+GGTN+C+RKFDA LI+ LI  H VTH+CGAPVVLNML+ 
Sbjct: 241 LWTLPIFHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSA 300

Query: 321 SPNNRPLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQL 380
           +   +PL RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTET GL + C+WK +WN+L
Sbjct: 301 TNEFQPLNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRL 360

Query: 381 PATERARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEA 440
           PA++RARLKARQGVRT+   E DV+D ESG+SV+ +G ++GEIV+RG S+MLGY KDP  
Sbjct: 361 PASDRARLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVG 420

Query: 441 TKKAMSEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEA 500
           T+KA+ + GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE 
Sbjct: 421 TEKAL-KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEV 480

Query: 501 AVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTS 560
           AVVARPD FWGETPCAFVSL+ GLT+ PTE E+IEYCR K+  +MVPKTV F+++LPKTS
Sbjct: 481 AVVARPDVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTS 540

Query: 561 TGKIQKFLLREMAKSMGPSRKSRL 577
           TGK+ KF+LRE+AK MG +R SR+
Sbjct: 541 TGKVMKFVLREIAKKMGTTRLSRM 552

BLAST of PI0020599 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 741.1 bits (1912), Expect = 6.7e-214
Identity = 358/550 (65.09%), Postives = 447/550 (81.27%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNS-TTYTWSQTYLRCLRLASSLSSI 80
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S T YTW +T LRCLR+ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 81  GIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQ 140
           GI +  VVSV++PNTPAMYELQFAVPM+GAIL+ IN RLDART+SV LRH ESKLLF+D 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 141 ASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEF 200
            S  L  +A+++    T  P+LV+IAD       A   ++     + +Y++++++GDP F
Sbjct: 121 FSVDLAVEAVSMMT--TDPPILVVIADKEEEGGVADVADLSK--FSYTYDDLIERGDPGF 180

Query: 201 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMF 260
           +WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK  VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 261 HANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRPL 320
           H+NGW+ P+ IAA+GGTN+C+RKFDA LI+ LI  H VTH+CGAPVVLNML+ +  ++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 321 ERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERAR 380
             PV ILTAG+PPPA VL R ES+GF +SHGYGLTET G+++ C+WK KWN LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 381 LKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMSE 440
           LKARQGVRT+   E DV+D ESG SV+ +G ++GEIV+RG S+MLGY KDP  T+KA+ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420

Query: 441 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 500
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 501 EFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 560
           EFWGETPCAFVSL+ G +  PTEEE++EYCR K+  +MVPKTV FM++LPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 561 LLREMAKSMG 570
           +LR++AK MG
Sbjct: 541 VLRDIAKKMG 545

BLAST of PI0020599 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 704.9 bits (1818), Expect = 5.3e-203
Identity = 339/550 (61.64%), Postives = 439/550 (79.82%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIYNSTTYTWSQTYLRCLRLASSLS--S 80
           ME L P  +NS PLT +GFLERA+ ++ DSPS+++ +T +TWS+T+ RCLR+AS+LS  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 81  IGIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFID 140
           +GI++G VVSVI PN P++YELQFAVPM+GA+L+ IN RLDA  +SV LRHSESKL+F+D
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 141 QASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPE 200
             SS+L+ +A++ F P  +RP LV++ D     D  SS     DF+ D+YE  +++GD  
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDG---NDMPSSSSADMDFL-DTYEGFMERGDLR 180

Query: 201 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPM 260
           F+W+RP SEW P+V+NYTSGTTSSPKGVVHSHR +F  T++SL++W +P + VYLWTLPM
Sbjct: 181 FKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPM 240

Query: 261 FHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNRP 320
           FHANGWS+ +A AA+G  NIC+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +P
Sbjct: 241 FHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKP 300

Query: 321 LERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERA 380
           L+ PVK++TAGAPPPA V+ + E+LGFDVSHGYG+TETGGLV+ C+ K +W++L   ERA
Sbjct: 301 LQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERA 360

Query: 381 RLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMS 440
           + K+RQG+RT   AE DV D  SGKSVK DG ++GEIV RGGS+MLGY+KDPE T  +M 
Sbjct: 361 KQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMR 420

Query: 441 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 500
           E+GWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNPA+ EAAVVA+P
Sbjct: 421 EDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKP 480

Query: 501 DEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQK 560
           D+ WGETPCAFVSL+     V TE EI E+C+ KL  +MVP+ VVF+E+LPKTSTGKIQK
Sbjct: 481 DKMWGETPCAFVSLKYHDGSV-TEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQK 540

Query: 561 FLLREMAKSM 569
           FLLR+MAKS+
Sbjct: 541 FLLRQMAKSL 544

BLAST of PI0020599 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 703.4 bits (1814), Expect = 1.5e-202
Identity = 351/550 (63.82%), Postives = 437/550 (79.45%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIY-NSTTYTWSQTYLRCLRLASSLSSI 80
           ME LKP+ ANS PLT +GFLERA+ +Y D  SI+Y NST YTW +T  RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 81  GIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFIDQ 140
           GI +  VVSV++ NTP MYELQF+VPM+GAIL+ IN RLDART+SV LRH ESKLLF+D 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 141 ASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDPEF 200
             S L  +A+ +       P+LVLIA++   E  A   E         Y +++ +G+P+F
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTE--RSKFCYLYSDLITRGNPDF 180

Query: 201 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLPMF 260
           +WIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIF +TLDSL +W VPK  VYLWTLP+F
Sbjct: 181 KWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIF 240

Query: 261 HANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNS-PNNRP 320
           HANGW++P+ IAA+GGTN+C+RK  A  I+ LI  H VTH+ GAP+VL +L+ S  +++P
Sbjct: 241 HANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQP 300

Query: 321 LERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERA 380
           L+ PV  LTAG+ PPA VL R ESLGF VSHGYGLTET G+++ C+WK  WN+LPA+++A
Sbjct: 301 LKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQA 360

Query: 381 RLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAMS 440
           +LK+RQGVRT+  +E DV+D ESG+SV+ DG ++GEIVLRG S+MLGY K+P  T+ +  
Sbjct: 361 QLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF- 420

Query: 441 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 500
           + GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARP
Sbjct: 421 KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARP 480

Query: 501 DEFWGETPCAFVSLREGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQK 560
           DEFWGETPCAFVSL+ GLTR PT++EIIEYC+ K+  +M PKTV F+E+LPKTSTGKI K
Sbjct: 481 DEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIK 540

Query: 561 FLLREMAKSM 569
            LL+E+AK+M
Sbjct: 541 SLLKEIAKNM 544

BLAST of PI0020599 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 699.5 bits (1804), Expect = 2.2e-201
Identity = 336/553 (60.76%), Postives = 439/553 (79.39%), Query Frame = 0

Query: 21  MEHLKPTPANSSPLTPIGFLERASIIYADSPSIIY-NSTTYTWSQTYLRCLRLASSL--S 80
           ME L P P+NS+PLT +GFL+RA+ +Y D PSI++  +T +TWS+T+ RCLR+AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 81  SIGIHKGHVVSVIAPNTPAMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFI 140
           S+GI++G VVSV+ PN P++YELQFAVPM+GAIL+ IN RLDA  +SV LRHSESKL+F+
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 141 DQASSALISDALALFPPHTKRPLLVLIADDTAVEDSASSVEIGGDFVADSYEEMVQKGDP 200
           D  S +++ +A++ F    ++P LVL+ DD   ED + S     DF+ D+Y+ ++++GD 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSAASDFL-DTYQGVMERGDS 180

Query: 201 EFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKQSVYLWTLP 260
            F+WIRP +EW P+++NYTSGTTSSPKGVV SHR IF +T+ SL++W  P + VYLWTLP
Sbjct: 181 RFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLP 240

Query: 261 MFHANGWSFPFAIAAIGGTNICIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPNNR 320
           MFHANGW + +  AA+G TN+C R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P+ +
Sbjct: 241 MFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRK 300

Query: 321 PLERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATER 380
           PL+ PV+++TAGAPPPAA++ R E+LGF+V HGYGLTETGG V+ C+WK +W+ L   ER
Sbjct: 301 PLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLER 360

Query: 381 ARLKARQGVRTLAMAEADVLDSESGKSVKPDGVSIGEIVLRGGSMMLGYFKDPEATKKAM 440
           ARLK+RQGVRT+  AE DV D  +GKSV+ DGVS+GEIVL+GGS+MLGY+KDPE T   M
Sbjct: 361 ARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACM 420

Query: 441 SEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVAR 500
            E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+
Sbjct: 421 REDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAK 480

Query: 501 PDEFWGETPCAFVSLR--EGLTRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGK 560
           PD+ WGETPCAFVSL+       + TE EI E+C+ +L  +MVP+ V+F E+LPKTSTGK
Sbjct: 481 PDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGK 540

Query: 561 IQKFLLREMAKSM 569
           IQKFLLR+MAKS+
Sbjct: 541 IQKFLLRQMAKSL 549