PI0020565 (gene) Melon (PI 482460) v1

Overview


Name	PI0020565
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	ubiquitin-conjugating enzyme E2 22
Location	chr03: 21852577 .. 21856960 (+)
RNA-Seq Expression	PI0020565
Synteny	PI0020565

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACGAGATTTCACTTCTGTCTTCAATTTTGCTCGAGGTCCATTGAATTAAGTTAGTCCTGAGATTTCACTTTTGTTTTCAATCTCACCTGCCATCTCACACATCTCCCCCAATTTCCTCAAATCTCGTTCGAGATTGCTTGAGGCAGCTGCCTGAGTCTTAACAAATCATCTATCTTTTGAACTTCATATCTCCCTCAATACTAACTCATTTTAAGTGTATGACCACTTGATTTGGTGCATTTTTTCATGATCTTTACATCTATAGTCATAAACGGAGTATTTTATTAATGTATCTTAAAATTGAATTTCCCTTATAATTATATATATTGAAAGATCATAACTTTGAGCATAGTCTTTGTTCAAATTGTGTGATTCTTTTTAATTCCATGTAACTTTTCGAGATCTACACTCTGGCAAGTGTCGAAGGCAACTTGTCCGACCCTTCATCCCAAAAAATGTCCATTTTGGCCCCCAAATTGGATTACACAATGTTTTTCATCAAAGTTTCATAGTTTTGAAGTTTGAAACTGCTAGCATATGGTTCTACTTTTTGTATCTTCTTTCTTTTATATGTAGTAACAATACAAGAACATAGAAAATTACGTGCGGTGGTACCTATATGTGTCTACCCGAGTTTTAAATCTTGAGGTAAAGTTGCCTCGAGATCACCCCGAGATTTAAAACTAGGGGCAAAGTTGCGCCGAGGTTTAAATCTCAAGTGATCTTGAGGCAAAATCTTGGGTGATCTCAAGGCAACTTTGCCCCAAGATTTAAAACTTGGATCGGGTCCTAGAGTCCATCCCTTAATGTGGCAACCAGTTTGATTCCACATATCGGCCACCTGAAACCGAAATCGTGGACTAGGTCCACAAGTTTATTTCAGATCTTTGAGAAATTACAGCATAAGGAAGTGAGGATGAAGTAGAGCATTTTATAGCATAAAAAAAGATAAATAAGAGTTCCAAAATTGAAGAAGAATTGAAAATTCCATAAAGAGAAATGAAAATCAAGTGGCAAATATATATGGAGAAATAAAAAGGGTGACTCCAAGTTCATTTGAAATTGCGGATATGGCAAATTAATTATATTGTTTTATTTTAATTTGCATTTATCATTTATTCTTTTTACTAATTTTTTCCTTTTTAATAAATCGATATATCGAAATTTTAACTCTCTCATTTACATATTTTCAATTTTTAAATCTTCTTGTTTTTTTCGTTGATCATTATGTTTTTTTTTTATATATATAATTTTATCTGTTTGACTATTTTTTAATTTTCGCCGTATATTATTTTTTTATTCCTTTCTTATTTTGAATTTTTTAATTCTTTTTCATCAGCATATTTTTAGAGGATTTTGTTGGAAGCGTTGACGAGCACACTCTAAAACTCACAAACTCTTAAGAGAAGAATCTTGTTCTCTTACAAAACTCAAAAACACACCAGAGAAGAATATTGTTCTCTTGAAACTCACTTTCTTTATAAACTTTACTTTGCTTCCATTTATATAATATTTTCTCTCTTTCTTATAAATGAGGGAAGTGAACTTTGTATATAGTACTTAAGAGACACTTCCTAAAAGACACAAAATAAATGAGGTACATGAATACATACCATTCATGTACTTGAATAATTACATGTATCTAGAAATGCATGTATCTGAAAATAGAAATGTATTTAAGAATGTGTTATCGATAATGAATCTAGCTTATTAAATTATAAAAGATTTGCTACCCGTTTCATATAAAAATACACTGACATGTGACTTCTAAATTTTAACATTTGCAAATATATAAAATCATATTTTATTGACTGCACGAGGTTTGCACAGGGTTTCTTCGTCGCTAGCCGTGTGTTTTTTTCTCCTCTCTTACTCTCAGTTTGTCTCTACAGCTTCAGGCACCATCAACTGCTTCTCCAATGTATCTGAATTCATCTTGTTTACCCCAACGTATTTTCTGGCGATTTTCTTACATATTTTAACACAACCGTAAGAGAAAAAAAAATCAATATTCACACTTATGCAACGGTACTCAAATTTACTTTCAACATCTACGTCAAAAGACAAATTTTCATTTGCATCAAGAGTTGATTAATATGTTTGATAGCACCCAAACTGTACTATCTAACTCCTATTTTGAGATCATTACATGATTTTACGTTTCTATTTCACTATTTGGTAGATTTTAAGCCAACTAAGGGTAGAATTCGACGTTGAGCTCAATTTAGAGCTAAAATGGACGAAAATGAAATCCTAAGGTATCAACGAATGTCCGGGACAAGACATGAAGAAGAGATGCCAAATTTGGTCTCTACCTTGTAGTGCTGTAGCGCTATGCAAGGCGTTGGAAGGCAGTAGCTACCTTACTCAAGAAAGCACATGGTGCCACGACGCTCTGAACCTGATGCTGCGTAAAATTTCCGTTAGCGCCGTGGCGCTACTTTAAAGTGTCGCGCCACCAGTTCGTTTTCTATAGAAATTGATCCGCTGCCTAAGTCAAGCCATCTGATCTGATTCCAGCTGTCCTTAACCCGCTTTCTCTCTATTTCCTAAGTTTTTTAGTGTAGTTTTATGCATTGAACATCTCTCCCTTGCTCGACATGACGAGAATCGGCTAAGGTACTCTATCTTGGCTTGACTTTGTAGCAATCACCCTTGAATTCATGCATGGGTGAGGTTTGGGGTTTTAGTAATGCGAACTGTTTTCTGAAATTAAATGCTTTTTCCCTGATGTTTGATTTTGGAGCTCTATTGGTATATTCTTGCATCGTGTCTGTTATCTTTGAATGCTTTGAAAATTATGCTCAAATCAGTGGTCGATTGCTATTGATTTCAAAGTTTGTTGGCTGTTTTTATTTTTTAGTATGAGGAACAATTTTCCTTTCTGTTCTTCATGTTGGTGATGTGACCTTTGTAGGATTATGCTTAACGTCTAGATCATAGAAGCTTAAAGACCTGGGAATGTGGAAGCTATTAATAGTTTGATTTTAGAGTTCTAGAGTTCTGAAGTTGAAATCAACCGTTATTTCTATAATGGCATCTATACTGCTCAATGTCTGAGGGTCTATCAGAAAGCAGTAATGTCTAGGATTGAATACTTAACTACGAATGCCCACCCTAGTCGTAATCTCTCCCCTCGTCTACTGAACTTGTTACAACCTAATTGTAATTACTTTTTCAAATTTCTGGCCGTAATTATGTTTCTTGCATGATATCCTCATTGACTGAAGAGAACTGTTTTTACAAATGCTTTAGTATATTGAATTCAGTTTAATATGTTTTGTTTGTTTCAGAATCTGTGTATGATAAATAGGTGTTATGTTGAACAGGTTGTTAGGTGTCTACTGGTTGAACCATTTCCAGAACCAGCTTTGAATGAACAAGCTGGCAGGCTGCTGCTTGAAAATTATGACGAGTATGCCAGACATGCCAAGTTAGTCAGGCCCTTCTCCAAATTGTCTGTTCTATGAATATAAAGGTGATATGAAATGCTGGAATGGTTATTATTGTTTATTTGGATAGACTTCATTTGATTTTATTGGTGCACCCATGTGATGTTGAACCCAATAATCACTATTCAAACTAAGATTGAACTTGCACGACTCTCGAAACGGTAAAAAGTTCTTTCAAAATATTCACGTCGGGTTGTACAACTATAATATTATGTGAAACTTTTAGAATCCCTGGAAACCGTGCAAGATGATGCACTTAAGAAAAGGCTTTTGCTGGAAACGATTTTCTTGTTTGACTCTATATTGGAAATTTTTGTTGGTTTCCAACCAGTTTTCTTAGTATGGTTTTTAATTTGAACACTAAGCTTAATTTATTTTCAAAAAAACCAAAGATCAAATAATGATTGAACCAAATCTATATCTGCTTTTCCACATCCACTCCTTTGTTCTATATTTGTTATAAAAAATTTAATTTTCTAGTAAATTGTCCTTTAAAAGATAATAATAATGATGGTATGATCAAAACAACAAGCAGCCAACTTTCCTCTTGCTTTCCCAGGCTCTATACAGGAATCCATGCAAAACCAAAGCCAAAATTCAAGATGGGAGCCATTTCCAAATCCACAACCGCCCTCAATGTCGACCCGACAAATGAGTCGGCACTTAAATACAGATCTGAAAAATGCATCTGCCACAGCATTGCCTTTGGCATGTCCAACTGCCAATTCTAATACTACAGTGAGAAGCGGGCAGGAGCAGCAGCAGCCAAATGTCGTTTGAGCACCAGCCTCGGAATCAGGAGTCGGGGGTTCCAGGGTGGCTGGAGCACCAGCAGCAGCGGTGAAGAAGGAAAGTGAACCTTTAAAAATGCAGCAGGTTGACAAGAAGAAGAAGATAGATGCAAGAAAGAAAAGGTTGAAGAGATTGTGA

mRNA sequence

CCACGAGATTTCACTTCTGTCTTCAATTTTGCTCGAGGTATCAACGAATGTCCGGGACAAGACATGAAGAAGAGATGCCAAATTTGGTCTCTACCTTGTAGTGCTGTAGCGCTATGCAAGGCGATTGAATACTTAACTACGAATGCCCACCCTAGTCGTAATCTCTCCCCTCGTCTACTGAACTTGTTACAACCTAATTGTAATTACTTTTTCAAATTTCTGGCCGTTGTTAGGTGTCTACTGGTTGAACCATTTCCAGAACCAGCTTTGAATGAACAAGCTGGCAGGCTGCTGCTTGAAAATTATGACGAGTATGCCAGACATGCCAAGCTCTATACAGGAATCCATGCAAAACCAAAGCCAAAATTCAAGATGGGAGCCATTTCCAAATCCACAACCGCCCTCAATGTCGACCCGACAAATGAGTCGGCACTTAAATACAGATCTGAAAAATGCATCTGCCACAGCATTGCCTTTGGCATGGTGGCTGGAGCACCAGCAGCAGCGGTGAAGAAGGAAAGTGAACCTTTAAAAATGCAGCAGGTTGACAAGAAGAAGAAGATAGATGCAAGAAAGAAAAGGTTGAAGAGATTGTGA

Coding sequence (CDS)

CCACGAGATTTCACTTCTGTCTTCAATTTTGCTCGAGGTATCAACGAATGTCCGGGACAAGACATGAAGAAGAGATGCCAAATTTGGTCTCTACCTTGTAGTGCTGTAGCGCTATGCAAGGCGATTGAATACTTAACTACGAATGCCCACCCTAGTCGTAATCTCTCCCCTCGTCTACTGAACTTGTTACAACCTAATTGTAATTACTTTTTCAAATTTCTGGCCGTTGTTAGGTGTCTACTGGTTGAACCATTTCCAGAACCAGCTTTGAATGAACAAGCTGGCAGGCTGCTGCTTGAAAATTATGACGAGTATGCCAGACATGCCAAGCTCTATACAGGAATCCATGCAAAACCAAAGCCAAAATTCAAGATGGGAGCCATTTCCAAATCCACAACCGCCCTCAATGTCGACCCGACAAATGAGTCGGCACTTAAATACAGATCTGAAAAATGCATCTGCCACAGCATTGCCTTTGGCATGGTGGCTGGAGCACCAGCAGCAGCGGTGAAGAAGGAAAGTGAACCTTTAAAAATGCAGCAGGTTGACAAGAAGAAGAAGATAGATGCAAGAAAGAAAAGGTTGAAGAGATTGTGA

Protein sequence

PRDFTSVFNFARGINECPGQDMKKRCQIWSLPCSAVALCKAIEYLTTNAHPSRNLSPRLLNLLQPNCNYFFKFLAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTTALNVDPTNESALKYRSEKCICHSIAFGMVAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL

Homology

[Close]

BLAST of PI0020565 vs. ExPASy Swiss-Prot
Match: Q9FF66 (Ubiquitin-conjugating enzyme E2 22 OS=Arabidopsis thaliana OX=3702 GN=UBC22 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.9e-29
Identity = 79/142 (55.63%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L+VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LSVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESA-------------LKYRSEKCICHSIAFGMVAGAPAAAV----KKESEP 193
           ALNV  TN                +K  +           +V  A A+ V    K+E+  
Sbjct: 172 ALNVGQTNNETPGAATAIPSSMTDIKRVTTSAQDQQHVANVVVAASASVVTTTQKREAGL 231

Query: 194 LKMQQVDKKKKIDARKKRLKRL 199
            K+Q    KKK+DARKK LKRL
Sbjct: 232 AKVQA--DKKKVDARKKSLKRL 251

[Close]

BLAST of PI0020565 vs. ExPASy Swiss-Prot
Match: A7SE05 (Ubiquitin-conjugating enzyme E2 S OS=Nematostella vectensis OX=45351 GN=v1g237158 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-09
Identity = 28/45 (62.22%), Postives = 37/45 (82.22%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAK 119
           L  V+CLL+ P PE ALNE+AG+LLLE YD+Y++ AK++T IHAK
Sbjct: 116 LLTVKCLLIVPNPESALNEEAGKLLLERYDDYSKRAKMFTEIHAK 160

[Close]

BLAST of PI0020565 vs. ExPASy Swiss-Prot
Match: Q76EZ2 (Ubiquitin-conjugating enzyme E2 S-C OS=Xenopus laevis OX=8355 GN=ube2s-c PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.1e-09
Identity = 48/125 (38.40%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L  ++CLL+ P PE ALNE+AGRLLLENY+EYA  AKL T IHA            + +T
Sbjct: 113 LLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRAKLMTEIHA------------QGST 172

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGMVAGAPAAAVKKESEPLKMQQVDKKKKIDARKK 193
               DPT+            C S +  +V+G    A K   +  + +++  KKK D +K+
Sbjct: 173 LRGKDPTDP-----------CSSASATVVSGDGPMAKKHAGD--RDKKLAAKKKTD-KKR 211

Query: 194 RLKRL 199
            L+RL
Sbjct: 233 ALRRL 211

[Close]

BLAST of PI0020565 vs. ExPASy Swiss-Prot
Match: Q8AVU2 (Ubiquitin-conjugating enzyme E2 S-B OS=Xenopus laevis OX=8355 GN=ube2s-b PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 8.7e-09
Identity = 47/125 (37.60%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L  ++CLL+ P PE ALNE+AGRLLLENY+EYA  A+L T IHA            + T+
Sbjct: 113 LLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRARLMTDIHA------------QGTS 172

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGMVAGAPAAAVKKESEPLKMQQVDKKKKIDARKK 193
               DPT+            C S +  +V+G    A K   +  + +++  KKK D +K+
Sbjct: 173 LRGKDPTDP-----------CSSASTPVVSGDGPMAKKHAGD--RDKKLAAKKKTD-KKR 211

Query: 194 RLKRL 199
            L+RL
Sbjct: 233 ALRRL 211

[Close]

BLAST of PI0020565 vs. ExPASy Swiss-Prot
Match: Q16763 (Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens OX=9606 GN=UBE2S PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.5e-08
Identity = 46/128 (35.94%), Postives = 62/128 (48.44%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIH---AKPKPKFKMG-AIS 133
           L  ++CLL+ P PE ALNE+AGRLLLENY+EYA  A+L T IH     P  + + G A++
Sbjct: 113 LLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRALA 172

Query: 134 KSTTALNVDPTNESALKYRSEKCICHSIAFGMVAGAPAAAVKKESEPLKMQQVDKKKKID 193
             T A + DP                        GAP      E    K    ++ KK+ 
Sbjct: 173 SGTEASSTDP------------------------GAPGGPGGAEGPMAKKHAGERDKKLA 216

Query: 194 ARKKRLKR 198
           A+KK  K+
Sbjct: 233 AKKKTDKK 216

[Close]

BLAST of PI0020565 vs. ExPASy TrEMBL
Match: A0A5D3BZH9 (Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00180 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.8e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 142 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKAGAISESTT 201

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGM-------------------------------- 193
           ALNVDPTN SAL    +     ++                                    
Sbjct: 202 ALNVDPTNASALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGV 261

Query: 194 --VAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQVD KKKIDARKK LKRL
Sbjct: 262 SGVGVAPAATVKKESGHLKMQQVD-KKKIDARKKSLKRL 299

[Close]

BLAST of PI0020565 vs. ExPASy TrEMBL
Match: A0A5A7VIJ3 (Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G00920 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.8e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 142 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 201

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGM-------------------------------- 193
           ALNVDPTN SAL    +     ++                                    
Sbjct: 202 ALNVDPTNASALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGV 261

Query: 194 --VAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQVD KKKIDARKK LKRL
Sbjct: 262 SGVGVAPAATVKKESGHLKMQQVD-KKKIDARKKSLKRL 299

[Close]

BLAST of PI0020565 vs. ExPASy TrEMBL
Match: A0A1S3CLW5 (ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo OX=3656 GN=LOC103502453 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.8e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGM-------------------------------- 193
           ALNVDPTN SAL    +     ++                                    
Sbjct: 172 ALNVDPTNASALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGV 231

Query: 194 --VAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQVD KKKIDARKK LKRL
Sbjct: 232 SGVGVAPAATVKKESGHLKMQQVD-KKKIDARKKSLKRL 269

[Close]

BLAST of PI0020565 vs. ExPASy TrEMBL
Match: A0A0A0L0F5 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G340550 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.0e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LTVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESALKYRSEKC-------IC---------------------------HSIAF 193
           ALNVDPTN SAL    +         +C                             +  
Sbjct: 172 ALNVDPTNASALSTDLKNASATALPLVCPTANSITVVRSGQEQQQPNVVTALASESGVGG 231

Query: 194 GMVAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQV  KKKIDARKK LKRL
Sbjct: 232 SGVGEAPAAMVKKESGHLKMQQVG-KKKIDARKKSLKRL 269

[Close]

BLAST of PI0020565 vs. ExPASy TrEMBL
Match: A0A6J1EAT8 (ubiquitin-conjugating enzyme E2 22-like OS=Cucurbita moschata OX=3662 GN=LOC111432427 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 4.9e-31
Identity = 93/158 (58.86%), Postives = 99/158 (62.66%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKSGAISESTT 171

Query: 134 ALNVDPTNESALK---------YRSEKC-----------------------ICHSIAFGM 193
           ALNVDPTN SAL            S  C                          S   G 
Sbjct: 172 ALNVDPTNASALNTDLKNTSAAVLSSACPTANSNTAVRGGQEQQPNTTTPPALESGVGGS 231

Query: 194 VAGAPAAA-VKKESEPLKMQQVDKKKKIDARKKRLKRL 199
              APAAA VKK+  PLKMQ    KKKIDARKK LKRL
Sbjct: 232 GVAAPAAAVVKKQDGPLKMQ--GDKKKIDARKKSLKRL 267

[Close]

BLAST of PI0020565 vs. NCBI nr
Match: XP_008464614.1 (PREDICTED: ubiquitin-conjugating enzyme E2 22 [Cucumis melo])

HSP 1 Score: 152.5 bits (384), Expect = 3.7e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGM-------------------------------- 193
           ALNVDPTN SAL    +     ++                                    
Sbjct: 172 ALNVDPTNASALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGV 231

Query: 194 --VAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQVD KKKIDARKK LKRL
Sbjct: 232 SGVGVAPAATVKKESGHLKMQQVD-KKKIDARKKSLKRL 269

[Close]

BLAST of PI0020565 vs. NCBI nr
Match: KAA0067923.1 (ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 3.7e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 142 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 201

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGM-------------------------------- 193
           ALNVDPTN SAL    +     ++                                    
Sbjct: 202 ALNVDPTNASALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGV 261

Query: 194 --VAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQVD KKKIDARKK LKRL
Sbjct: 262 SGVGVAPAATVKKESGHLKMQQVD-KKKIDARKKSLKRL 299

[Close]

BLAST of PI0020565 vs. NCBI nr
Match: TYK04292.1 (ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 3.7e-33
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 142 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKAGAISESTT 201

Query: 134 ALNVDPTNESALKYRSEKCICHSIAFGM-------------------------------- 193
           ALNVDPTN SAL    +     ++                                    
Sbjct: 202 ALNVDPTNASALSTDLKNASATALPLACPTANSTTAVRSGQEQQQPNVVTALASESGVGV 261

Query: 194 --VAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQVD KKKIDARKK LKRL
Sbjct: 262 SGVGVAPAATVKKESGHLKMQQVD-KKKIDARKKSLKRL 299

[Close]

BLAST of PI0020565 vs. NCBI nr
Match: XP_004142654.1 (ubiquitin-conjugating enzyme E2 22 [Cucumis sativus] >KGN54504.1 hypothetical protein Csa_011956 [Cucumis sativus])

HSP 1 Score: 150.2 bits (378), Expect = 1.9e-32
Identity = 93/159 (58.49%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LTVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESALKYRSEKC-------IC---------------------------HSIAF 193
           ALNVDPTN SAL    +         +C                             +  
Sbjct: 172 ALNVDPTNASALSTDLKNASATALPLVCPTANSITVVRSGQEQQQPNVVTALASESGVGG 231

Query: 194 GMVAGAPAAAVKKESEPLKMQQVDKKKKIDARKKRLKRL 199
             V  APAA VKKES  LKMQQV  KKKIDARKK LKRL
Sbjct: 232 SGVGEAPAAMVKKESGHLKMQQVG-KKKIDARKKSLKRL 269

[Close]

BLAST of PI0020565 vs. NCBI nr
Match: XP_022925072.1 (ubiquitin-conjugating enzyme E2 22-like [Cucurbita moschata])

HSP 1 Score: 144.4 bits (363), Expect = 1.0e-30
Identity = 93/158 (58.86%), Postives = 99/158 (62.66%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LIVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKSGAISESTT 171

Query: 134 ALNVDPTNESALK---------YRSEKC-----------------------ICHSIAFGM 193
           ALNVDPTN SAL            S  C                          S   G 
Sbjct: 172 ALNVDPTNASALNTDLKNTSAAVLSSACPTANSNTAVRGGQEQQPNTTTPPALESGVGGS 231

Query: 194 VAGAPAAA-VKKESEPLKMQQVDKKKKIDARKKRLKRL 199
              APAAA VKK+  PLKMQ    KKKIDARKK LKRL
Sbjct: 232 GVAAPAAAVVKKQDGPLKMQ--GDKKKIDARKKSLKRL 267

[Close]

BLAST of PI0020565 vs. TAIR 10
Match: AT5G05080.1 (ubiquitin-conjugating enzyme 22 )

HSP 1 Score: 128.3 bits (321), Expect = 7.1e-30
Identity = 79/142 (55.63%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L+VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LSVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESA-------------LKYRSEKCICHSIAFGMVAGAPAAAV----KKESEP 193
           ALNV  TN                +K  +           +V  A A+ V    K+E+  
Sbjct: 172 ALNVGQTNNETPGAATAIPSSMTDIKRVTTSAQDQQHVANVVVAASASVVTTTQKREAGL 231

Query: 194 LKMQQVDKKKKIDARKKRLKRL 199
            K+Q    KKK+DARKK LKRL
Sbjct: 232 AKVQA--DKKKVDARKKSLKRL 251

[Close]

BLAST of PI0020565 vs. TAIR 10
Match: AT5G05080.2 (ubiquitin-conjugating enzyme 22 )

HSP 1 Score: 128.3 bits (321), Expect = 7.1e-30
Identity = 79/142 (55.63%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 74  LAVVRCLLVEPFPEPALNEQAGRLLLENYDEYARHAKLYTGIHAKPKPKFKMGAISKSTT 133
           L+VVRCLL+EPFPE ALNEQAG++LLENYDEYARHA+LYTGIHAKPKPKFK GAIS+STT
Sbjct: 112 LSVVRCLLIEPFPESALNEQAGKMLLENYDEYARHARLYTGIHAKPKPKFKTGAISESTT 171

Query: 134 ALNVDPTNESA-------------LKYRSEKCICHSIAFGMVAGAPAAAV----KKESEP 193
           ALNV  TN                +K  +           +V  A A+ V    K+E+  
Sbjct: 172 ALNVGQTNNETPGAATAIPSSMTDIKRVTTSAQDQQHVANVVVAASASVVTTTQKREAGL 231

Query: 194 LKMQQVDKKKKIDARKKRLKRL 199
            K+Q    KKK+DARKK LKRL
Sbjct: 232 AKVQA--DKKKVDARKKSLKRL 251

The following BLAST results are available for this feature:

BLAST of PI0020565 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FF66	9.9e-29	55.63	Ubiquitin-conjugating enzyme E2 22 OS=Arabidopsis thaliana OX=3702 GN=UBC22 PE=1...	[more]
A7SE05	1.8e-09	62.22	Ubiquitin-conjugating enzyme E2 S OS=Nematostella vectensis OX=45351 GN=v1g23715...	[more]
Q76EZ2	5.1e-09	38.40	Ubiquitin-conjugating enzyme E2 S-C OS=Xenopus laevis OX=8355 GN=ube2s-c PE=2 SV...	[more]
Q8AVU2	8.7e-09	37.60	Ubiquitin-conjugating enzyme E2 S-B OS=Xenopus laevis OX=8355 GN=ube2s-b PE=2 SV...	[more]
Q16763	1.5e-08	35.94	Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens OX=9606 GN=UBE2S PE=1 SV=2	[more]

BLAST of PI0020565 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BZH9	1.8e-33	58.49	Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A5A7VIJ3	1.8e-33	58.49	Ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3CLW5	1.8e-33	58.49	ubiquitin-conjugating enzyme E2 22 OS=Cucumis melo OX=3656 GN=LOC103502453 PE=3 ...	[more]
A0A0A0L0F5	9.0e-33	58.49	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G340550 PE...	[more]
A0A6J1EAT8	4.9e-31	58.86	ubiquitin-conjugating enzyme E2 22-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of PI0020565 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008464614.1	3.7e-33	58.49	PREDICTED: ubiquitin-conjugating enzyme E2 22 [Cucumis melo]	[more]
KAA0067923.1	3.7e-33	58.49	ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa]	[more]
TYK04292.1	3.7e-33	58.49	ubiquitin-conjugating enzyme E2 22 [Cucumis melo var. makuwa]	[more]
XP_004142654.1	1.9e-32	58.49	ubiquitin-conjugating enzyme E2 22 [Cucumis sativus] >KGN54504.1 hypothetical pr...	[more]
XP_022925072.1	1.0e-30	58.86	ubiquitin-conjugating enzyme E2 22-like [Cucurbita moschata]	[more]

BLAST of PI0020565 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05080.1	7.1e-30	55.63	ubiquitin-conjugating enzyme 22	[more]
AT5G05080.2	7.1e-30	55.63	ubiquitin-conjugating enzyme 22	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 34..130 e-value: 7.1E-6 score: 27.5
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 72..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 176..198
None	No IPR available	PANTHER	PTHR24068:SF377	BNAA03G55110D PROTEIN	coord: 73..141
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 73..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0020565.1	PI0020565.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity