PI0020248 (gene) Melon (PI 482460) v1

Overview
NamePI0020248
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProtein kinase domain-containing protein
Locationchr08: 14384990 .. 14388571 (+)
RNA-Seq ExpressionPI0020248
SyntenyPI0020248
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCATGAAATATGATCTCGTTGGATGGTTCATATATTAATTATTGTGCAGTTTAGAGAAATTCTAGGCAAAGGAGCCACGAAAACAGTATATAAAGGCTTCGATGAGGTTCTTGGAATTGAAGTGGCTTGGAACCAAGTCCATCTCAAAGACGTCTTCCATTCCCCTGAAGAACTACAACGTTTATACTCAGAAGTTCATCTCCTCAAGAACCTCAACCACGACTCGATCATCCGATTCTACACTTCTTGGATCGATACTCATCGCCGAACATTCAACTTCATCACCGAAATGTTCACGTCTGGCACCCTTAGAGAGTACTGACTAATTCTCGACCATATGTTTTTGTTAATTCTCACTTTTGTTCAATTGGGGGGATGATCATCGTCAATGCTATTAGGTACCGACAAAAGTATCGAAACATCGACATCGAAGCAATAAAGAACTGGGCTCGTCAAATCCTACACGGCCTTGTTTATCTTCATGGTCATGATCCACCCGTAATTCATAGAGACCTAAAGTGTGATAATGTATTCATCAATGGCCATCTCGGGCAAGTTAAAATTGGTGACCTTGGACTCGCAACAATCCTTCATGATTCTCAGCATGCTCATAGTGTCATAGGTAACCTTCTAAATTTAATATTGAGATGATAGGACCTCTTCGTATTTCTACAATAGTATAATATAGTTGTCTGTTTAGAATGTTTGCACGTTCAATGACTCGAAGTAATGTCAAAACGTTTAATTAAGAATGGTTATGTGCTGAAAAAATTTTTAGGTACACCAGAGTTCATGGCACCAGAGCTTTATGATGAGGAATATAATGAACTTGTGGATGTTTATTCGTTTGGAATGTGTATGATTGAGATGCTTACTTTGGAGTATCCTTATAACGAGTGCTCTAACCCTGCTCAGATATACAAGAAAGTCACTTCAGTAAGTCAATATTATTATTACTAAAAAAATTCAATTATAATAAGTTTGATCCATTTTCAAGTGTGTCGATTTAGTAACAAAGTAGTTTTGAATAATTTATTGAACTTTTGAGAAAGTTGTATCGGTGATTTATTTGACAAGGTCCAAATGAAATATGATCCTACTTTTTGAAAACTCAAGTGTTATAACCTTTTGTTACATCATGTGATAAAGTGAAATACAATTAAAATCATCTAATCGTATACTAACGTTGATATTTGACTCTCACTCTGGTTAATTGCTATCTCCTTGTATGCTCGCACTCTCGTTACTAAGAAAGCTTGTTGAGTTTTCTTTTCCTCAATTTTAACATGTTTCTTCTCCGTTGCCTATCTTCTTAGCAAGTAGCAAGAATCGAATATATTTGCACGTGTATACAACAATAATGTCGATTCCATTATTTCACTTCATAGTTACGTAAGGAAAATGACAAAAAAAAAAAAAAAAATATGGATCAATTTAATTTAGTTGAAAACTAATCTTATAATTACCATAAGATGTTGGACTTAATAAAGAAATTGCAGGGAAAATTGCCCAATGCATTCTATGAGATCAAAGACTTGGAGGCCCAAAGATTTGTAAGAAAATGTTTGGAGAATGTTTCAAAGAGGGTATCTGCTAGAGAGCTCTTGCTTGATCCATTTCTAGCACCTCCTAACAGTAACAATGCCTCTCATAATGAAGAGTTGTTCTCTTCATCTTTATCTCCAGAGAAATCAATGTCAAGAAGAACAGATTTAGCCATTTCTGGATCAATCAATCCCAAGGATGATTCAATTTTTCTAAAAGTGCAGATCAAAGTGAAGAATGGTAAATTAATACGAGTTCTGATCTTGTTCAATTTTGAGTACATTACGTTAACAATAATTTGCAAAAACTCATTAATTATATATGAATATATATATATATATAGGTAAATCTAAGAATGTATATTTTGCATTCGACACCTTAAACGACACTGCAATCGACGTTGCGACCGAGATGGTTAAAGAGTTGGAAATTATCAGTGATTGGGATCCATTGGAGATAGCTGTGATGATAGAGAAGGAGATATCCTCATTGGTTCCAGATCGGGAGGAATGGAAGTTACAAAAAATCCACCATCAAGATAGCTTCAACTACGAACAAGATCACCATGGTGGCGACGATAACTACAATGACGATGACGACGAAAACTATGCAACACCACATCCTTTCTATTATTGTGGCTCTTCTCATGGCTCCTCCTCAGATTCTCTTCATGCTTTCTACTCTTTCCATGAGAACCCCAATCATCATTTTGGAGGAACGAAGGACACTTCTATTGCCACTGAATGGTTTCAAGGTACACCCTTTTCATTCTTATATCATTATGGGATTTTGTCCGATCGTTTGTTAATCATTTGGTTTTTTACTTCTAGTTTTTTTCAATTAAGCCTATAAATACTTTTCCCCTTCTAAATAATTCTTTGTTTTGTTATCTACTTTTTGCCAATATTTAAAAAATCTACGGTAAGTTTTGAAAATTAAAAAAAAAAAAGTTTTTTAAAACATGTTTTTATTTTTAAAATTTAGCTAAGAATTTAACTATATTACTTAAGAATGATGGGAACTATTATAAGTAAATGACGATAAACTTCATTTAAAAAAAAAAAAAACGGATTACCAAACATATAATTTTAGTTGTTATTTATTTTTTGGGTAAAATTCTAGGTTCGATTTTATAAGTTTTAGTAGGTCTTCCATCCCTCTAAATTAAAACGTTTAGAAGAAGCACCTTAAAAGTTTAAGGTTGTATCTACTTAGTCTACAATAATTTTCAATTGGTATGGCACCGGGTGAAACTACTGATTATAGAGTAAATGGTAGGAAACCCATATTTTAAGCATTTAAGTCTTTTTCGCTGCATGTGAAAAACAAATACTGTAGTGTTCTAAACACTAGTAAAGGTAATACTCTTGAGGTTGGTTTTAAATGCAGAGGATGATACAAGTTCTTGTTGCTCTCTCAATTCCTTCAATTACTCTGATTTAAGTTTTTACTCAAACAATGAAGATGAGTATGAATATGATTCAAATATCAAAGGAAGAGAGCCTCAATACATCTCAACAACCAAGCAACCTACAAGATTTTGCCCAACCATGAAGATAGACTCACACCATTTGAGGCACAAGGACACTAAAATCCCCAATCGAGAAATATTCGAGAGTCGATCTCGATCAAATAACTCGCCTAGACTTACGAGGGTGAAATCGATGGTGAACCTCCGTAGTGAGACGTTGCACCGATGCTTAGTAGAGATGTTGTTGAAGAAGCGCTTGTTTAATACGGTGGGTGCAATGGAGAATATTGGATACCAAAAGCCATAGCAAAATTAATATTATTATTCAATGTGTTCAAGTTTCATAAATTATTTCAATGGGATTTGAGGATTTGTGTATATAAATAAATGAAAAGGAAACTGAATTGTGTTTTGGGGCTTGTAGTAATTGTTTTGCATTTGTAAAACGTGTGAAACATAAAGCCATGGATACATATGATTGAATAGTATTGCCTTTTTAATGCATTTTTATGTGTAAAAGGAAACGTTTATCTTAAGAATATATTGAGTGTTCAAAGCTTTAAAA

mRNA sequence

TTCATGAAATATGATCTCGTTGGATGGTTCATATATTAATTATTGTGCAGTTTAGAGAAATTCTAGGCAAAGGAGCCACGAAAACAGTATATAAAGGCTTCGATGAGGTTCTTGGAATTGAAGTGGCTTGGAACCAAGTCCATCTCAAAGACGTCTTCCATTCCCCTGAAGAACTACAACGTTTATACTCAGAAGTTCATCTCCTCAAGAACCTCAACCACGACTCGATCATCCGATTCTACACTTCTTGGATCGATACTCATCGCCGAACATTCAACTTCATCACCGAAATGTTCACGTCTGGCACCCTTAGAGAGTACCGACAAAAGTATCGAAACATCGACATCGAAGCAATAAAGAACTGGGCTCGTCAAATCCTACACGGCCTTGTTTATCTTCATGGTCATGATCCACCCGTAATTCATAGAGACCTAAAGTGTGATAATGTATTCATCAATGGCCATCTCGGGCAAGTTAAAATTGGTGACCTTGGACTCGCAACAATCCTTCATGATTCTCAGCATGCTCATAGTGTCATAGGTACACCAGAGTTCATGGCACCAGAGCTTTATGATGAGGAATATAATGAACTTGTGGATGTTTATTCGTTTGGAATGTGTATGATTGAGATGCTTACTTTGGAGTATCCTTATAACGAGTGCTCTAACCCTGCTCAGATATACAAGAAAGTCACTTCAGGAAAATTGCCCAATGCATTCTATGAGATCAAAGACTTGGAGGCCCAAAGATTTGTAAGAAAATGTTTGGAGAATGTTTCAAAGAGGGTATCTGCTAGAGAGCTCTTGCTTGATCCATTTCTAGCACCTCCTAACAGTAACAATGCCTCTCATAATGAAGAGTTGTTCTCTTCATCTTTATCTCCAGAGAAATCAATGTCAAGAAGAACAGATTTAGCCATTTCTGGATCAATCAATCCCAAGGATGATTCAATTTTTCTAAAAGTGCAGATCAAAGTGAAGAATGGTAAATCTAAGAATGTATATTTTGCATTCGACACCTTAAACGACACTGCAATCGACGTTGCGACCGAGATGGTTAAAGAGTTGGAAATTATCAGTGATTGGGATCCATTGGAGATAGCTGTGATGATAGAGAAGGAGATATCCTCATTGGTTCCAGATCGGGAGGAATGGAAGTTACAAAAAATCCACCATCAAGATAGCTTCAACTACGAACAAGATCACCATGGTGGCGACGATAACTACAATGACGATGACGACGAAAACTATGCAACACCACATCCTTTCTATTATTGTGGCTCTTCTCATGGCTCCTCCTCAGATTCTCTTCATGCTTTCTACTCTTTCCATGAGAACCCCAATCATCATTTTGGAGGAACGAAGGACACTTCTATTGCCACTGAATGGTTTCAAGAGGATGATACAAGTTCTTGTTGCTCTCTCAATTCCTTCAATTACTCTGATTTAAGTTTTTACTCAAACAATGAAGATGAGTATGAATATGATTCAAATATCAAAGGAAGAGAGCCTCAATACATCTCAACAACCAAGCAACCTACAAGATTTTGCCCAACCATGAAGATAGACTCACACCATTTGAGGCACAAGGACACTAAAATCCCCAATCGAGAAATATTCGAGAGTCGATCTCGATCAAATAACTCGCCTAGACTTACGAGGGTGAAATCGATGGTGAACCTCCGTAGTGAGACGTTGCACCGATGCTTAGTAGAGATGTTGTTGAAGAAGCGCTTGTTTAATACGGTGGGTGCAATGGAGAATATTGGATACCAAAAGCCATAGCAAAATTAATATTATTATTCAATGTGTTCAAGTTTCATAAATTATTTCAATGGGATTTGAGGATTTGTGTATATAAATAAATGAAAAGGAAACTGAATTGTGTTTTGGGGCTTGTAGTAATTGTTTTGCATTTGTAAAACGTGTGAAACATAAAGCCATGGATACATATGATTGAATAGTATTGCCTTTTTAATGCATTTTTATGTGTAAAAGGAAACGTTTATCTTAAGAATATATTGAGTGTTCAAAGCTTTAAAA

Coding sequence (CDS)

ATGGTTCATATATTAATTATTGTGCAGTTTAGAGAAATTCTAGGCAAAGGAGCCACGAAAACAGTATATAAAGGCTTCGATGAGGTTCTTGGAATTGAAGTGGCTTGGAACCAAGTCCATCTCAAAGACGTCTTCCATTCCCCTGAAGAACTACAACGTTTATACTCAGAAGTTCATCTCCTCAAGAACCTCAACCACGACTCGATCATCCGATTCTACACTTCTTGGATCGATACTCATCGCCGAACATTCAACTTCATCACCGAAATGTTCACGTCTGGCACCCTTAGAGAGTACCGACAAAAGTATCGAAACATCGACATCGAAGCAATAAAGAACTGGGCTCGTCAAATCCTACACGGCCTTGTTTATCTTCATGGTCATGATCCACCCGTAATTCATAGAGACCTAAAGTGTGATAATGTATTCATCAATGGCCATCTCGGGCAAGTTAAAATTGGTGACCTTGGACTCGCAACAATCCTTCATGATTCTCAGCATGCTCATAGTGTCATAGGTACACCAGAGTTCATGGCACCAGAGCTTTATGATGAGGAATATAATGAACTTGTGGATGTTTATTCGTTTGGAATGTGTATGATTGAGATGCTTACTTTGGAGTATCCTTATAACGAGTGCTCTAACCCTGCTCAGATATACAAGAAAGTCACTTCAGGAAAATTGCCCAATGCATTCTATGAGATCAAAGACTTGGAGGCCCAAAGATTTGTAAGAAAATGTTTGGAGAATGTTTCAAAGAGGGTATCTGCTAGAGAGCTCTTGCTTGATCCATTTCTAGCACCTCCTAACAGTAACAATGCCTCTCATAATGAAGAGTTGTTCTCTTCATCTTTATCTCCAGAGAAATCAATGTCAAGAAGAACAGATTTAGCCATTTCTGGATCAATCAATCCCAAGGATGATTCAATTTTTCTAAAAGTGCAGATCAAAGTGAAGAATGGTAAATCTAAGAATGTATATTTTGCATTCGACACCTTAAACGACACTGCAATCGACGTTGCGACCGAGATGGTTAAAGAGTTGGAAATTATCAGTGATTGGGATCCATTGGAGATAGCTGTGATGATAGAGAAGGAGATATCCTCATTGGTTCCAGATCGGGAGGAATGGAAGTTACAAAAAATCCACCATCAAGATAGCTTCAACTACGAACAAGATCACCATGGTGGCGACGATAACTACAATGACGATGACGACGAAAACTATGCAACACCACATCCTTTCTATTATTGTGGCTCTTCTCATGGCTCCTCCTCAGATTCTCTTCATGCTTTCTACTCTTTCCATGAGAACCCCAATCATCATTTTGGAGGAACGAAGGACACTTCTATTGCCACTGAATGGTTTCAAGAGGATGATACAAGTTCTTGTTGCTCTCTCAATTCCTTCAATTACTCTGATTTAAGTTTTTACTCAAACAATGAAGATGAGTATGAATATGATTCAAATATCAAAGGAAGAGAGCCTCAATACATCTCAACAACCAAGCAACCTACAAGATTTTGCCCAACCATGAAGATAGACTCACACCATTTGAGGCACAAGGACACTAAAATCCCCAATCGAGAAATATTCGAGAGTCGATCTCGATCAAATAACTCGCCTAGACTTACGAGGGTGAAATCGATGGTGAACCTCCGTAGTGAGACGTTGCACCGATGCTTAGTAGAGATGTTGTTGAAGAAGCGCTTGTTTAATACGGTGGGTGCAATGGAGAATATTGGATACCAAAAGCCATAG

Protein sequence

MVHILIIVQFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGHDPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCLENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKSMSRRTDLAISGSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTKIPNREIFESRSRSNNSPRLTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP
Homology
BLAST of PI0020248 vs. ExPASy Swiss-Prot
Match: Q9LVL5 (Probable serine/threonine-protein kinase WNK4 OS=Arabidopsis thaliana OX=3702 GN=WNK4 PE=1 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.3e-148
Identity = 305/589 (51.78%), Postives = 387/589 (65.70%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +F EILG+GA KTVYK  DE LGIEVAW+QV LK+V  S  +LQRLYSEVHLL  LNH S
Sbjct: 20  RFAEILGRGAMKTVYKAIDEKLGIEVAWSQVKLKEVLRSSVDLQRLYSEVHLLSTLNHKS 79

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWID H  T NFITE+FTSGTLR+Y+ KY  IDI AIK+WARQIL GLVYLH H
Sbjct: 80  IIRFYTSWIDVHNHTLNFITELFTSGTLRQYKNKYLRIDIRAIKSWARQILEGLVYLHEH 139

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+F+NGHLGQVKIGDLGLA +L D   AHS+IGTPEFMAPELY+E YN
Sbjct: 140 DPPVIHRDLKCDNIFVNGHLGQVKIGDLGLARMLRDCHSAHSIIGTPEFMAPELYEENYN 199

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           EL+DVYSFGMC +EM+T E+PY+EC++PAQIYKKV  GKLP AFY + D+EAQRF+ KCL
Sbjct: 200 ELIDVYSFGMCFLEMITSEFPYSECNHPAQIYKKVVGGKLPGAFYRVGDIEAQRFIGKCL 259

Query: 249 ENVSKRVSARELLLDPFLAPPNS----NNASHNEELFSSSLSPE----KSMSRRTDLAIS 308
            + SKRVSA+ELL DPFLA   S     + + N + F +    +    +    RT+++I+
Sbjct: 260 VSASKRVSAKELLQDPFLASDESWMVYTSGAGNPKPFLNENEMDTLKLEDDELRTEMSIA 319

Query: 309 GSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIA 368
           G +  +D+ I L+VQI   NG + NV+F FD +NDT+IDVA EMVKELEII DW+P+EIA
Sbjct: 320 GKLGAEDNKIDLEVQIAYDNGLANNVFFPFDIMNDTSIDVAKEMVKELEII-DWEPVEIA 379

Query: 369 VMIEKEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGS 428
            MI+  ISSLV D   WK           YE+D     D++    D         ++  S
Sbjct: 380 KMIDGAISSLVSD---WK-----------YEEDDETPHDHHRHRTDS--------FHSSS 439

Query: 429 SHGSSSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQE---DDTSSCCSLNSFNYSDLSF 488
           SH SSS +           N+   G +D      W Q+   D+T S  S +S +YS+L++
Sbjct: 440 SHASSSQA--------SLSNYMARGLQD------WVQDDLHDETYSQSSSHSGSYSNLNY 499

Query: 489 YSNNEDEYEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTKIPNREIFESRSR 548
            +   DEY   S +       +S T   TRFCP    +S HL+             +RS 
Sbjct: 500 IA--VDEYSSQSPV-------MSRTHNMTRFCPE---ESSHLQSGQANAYAASSSTNRSL 559

Query: 549 SNNSPRLTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           ++++  LTR +S+V+++ + LHR   E   K+RLF TVG +E +G+Q P
Sbjct: 560 ASDNRTLTRNRSLVDVQRQLLHRSPGEEARKRRLFKTVGDVETVGFQSP 559

BLAST of PI0020248 vs. ExPASy Swiss-Prot
Match: Q9SCU5 (Probable serine/threonine-protein kinase WNK5 OS=Arabidopsis thaliana OX=3702 GN=WNK5 PE=1 SV=2)

HSP 1 Score: 516.9 bits (1330), Expect = 2.9e-145
Identity = 264/406 (65.02%), Postives = 324/406 (79.80%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FRE+LGKGA KTVYK FD+VLG+EVAWNQV L +VF SPE LQRLYSEVHLLKNLNH+S
Sbjct: 26  RFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLYSEVHLLKNLNHES 85

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIR+ TSWID +RRTFNFITE+FTSGTLREYR+KY+ +DI AIK+WARQIL+GL YLHGH
Sbjct: 86  IIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWARQILNGLAYLHGH 145

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+F+NGHLGQVKIGDLGLA IL  SQ+AHSVIGTPEFMAPELY+E+YN
Sbjct: 146 DPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTPEFMAPELYEEDYN 205

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVD+YSFGMC++EMLT EYPY+EC+NPAQIYKKVTSGKLP++F+ I+  EAQRFV KCL
Sbjct: 206 ELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLIQHTEAQRFVGKCL 265

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASH----NEELFSSSLSPEKSM----------SRR 308
           E VS+R+ A+ELL DPFLA  +  + +      ++L   +L+   ++          +R 
Sbjct: 266 ETVSRRLPAKELLADPFLAATDERDLAPLFRLPQQLAIQNLAANGTVVEHLPSTTDPTRT 325

Query: 309 TDLAISGSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDW 368
           TD++I+G +N +D +IFL+VQI   +G  +N+ F F+ L+DT ++VA EMVKELE I+DW
Sbjct: 326 TDMSITGKMNSEDHTIFLQVQILDGDGHMRNIQFPFNILSDTPLEVALEMVKELE-ITDW 385

Query: 369 DPLEIAVMIEKEISSLVPDREEWKL--QKIHHQDSFNYEQDHHGGD 399
           DPLEIA MIE EIS LVP+   W+     I H +SF +E D   GD
Sbjct: 386 DPLEIAAMIENEISLLVPN---WRANDSSIRH-ESFGHEDDEDNGD 426

BLAST of PI0020248 vs. ExPASy Swiss-Prot
Match: Q2QYL8 (Probable serine/threonine-protein kinase WNK8 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK8 PE=2 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.9e-128
Identity = 280/597 (46.90%), Postives = 361/597 (60.47%), Query Frame = 0

Query: 12  EILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDSIIR 71
           E+LGKGA KTVY+GFDE+ G+EVAWNQ  + DV  +P+ L R+Y+EV LL +L HD+II 
Sbjct: 39  EVLGKGAMKTVYRGFDELRGVEVAWNQATISDVLRTPDALHRMYAEVSLLADLRHDAIIA 98

Query: 72  FYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGHDPP 131
           F+ SW+   RRTFNFITE+F+SGTLR YR +Y  +   A+  WAR IL GL YLH     
Sbjct: 99  FHASWVHPSRRTFNFITELFSSGTLRSYRLRYPRVSRRAVAAWARAILRGLAYLHSRG-- 158

Query: 132 VIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYNELV 191
           VIHRDLKCDN+F+NGHLGQVKIGDLGLA +L     A SVIGTPEFMAPE+YDE Y   V
Sbjct: 159 VIHRDLKCDNIFVNGHLGQVKIGDLGLAAVLRGCTSARSVIGTPEFMAPEMYDECYGVGV 218

Query: 192 DVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCLENV 251
           DVYSFGMCM+EMLT EYPY+EC NPAQIYKKVT+GKLP+AFY + D +A+RF+ +CL + 
Sbjct: 219 DVYSFGMCMLEMLTNEYPYSECDNPAQIYKKVTAGKLPDAFYLLTDADARRFIGRCLVDA 278

Query: 252 SKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSP----------------EKSMSRRT 311
           + R SA ELLLDPFL+PP  N+  HN    +++  P                E   ++ T
Sbjct: 279 AHRPSAEELLLDPFLSPP-QNHDDHNTIAHATAPPPPLPLACSNSSEEQEEEEAPAAKTT 338

Query: 312 DLAISGSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWD 371
            +AI+G +N + D+IFLKVQI    G  +N+YF FD  NDTA++VATEMVKEL+ I+D +
Sbjct: 339 GMAITGKLNKEHDTIFLKVQIG-GGGNVRNIYFPFDVANDTAMEVATEMVKELD-IADRE 398

Query: 372 PLEIAVMIEKEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPF 431
           P EIA MIE+EI  LVP          + Q  ++Y  D         DDDD+    P+PF
Sbjct: 399 PTEIAAMIEQEIVRLVPG---------YKQHEYSYADD---------DDDDDVNGQPNPF 458

Query: 432 YYCGSSHGSSSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDL 491
           YY  SS  SS  SL              G T          + D +   C      Y   
Sbjct: 459 YYLSSSPTSSQGSLCG-----------VGPTSSEGFPGPHGKVDWSRDYC------YYPP 518

Query: 492 SFYSNNEDEYEYDSNIKGR------EPQYISTTKQPTRFCPTMKIDSHHLRHKDTKIPNR 551
           S  S ++D+    S++         + Q+ S +   +R  P     S        +   R
Sbjct: 519 SSVSVSDDDDSSTSSLSAAVSAISLQQQHCSASS--SRLGPASASASEDGGGHAGRPRQR 578

Query: 552 EIFESRSRSNNSPRLTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           E  E R R     R++R +SMV++RS+ LHR LVE L K+  FNTVGA+ +IG++ P
Sbjct: 579 EGEEERRRR----RMSRNRSMVDMRSQLLHRTLVEELNKRLFFNTVGAVHDIGFRDP 589

BLAST of PI0020248 vs. ExPASy Swiss-Prot
Match: Q2RBE3 (Probable serine/threonine-protein kinase WNK7 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK7 PE=2 SV=2)

HSP 1 Score: 454.9 bits (1169), Expect = 1.4e-126
Identity = 287/602 (47.67%), Postives = 372/602 (61.79%), Query Frame = 0

Query: 12  EILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDSIIR 71
           E+LGKGA KTVY+GFDE+ G+EVAWNQ  + DV  +P+ L R+Y+EV LL +L HD+II 
Sbjct: 39  EVLGKGAMKTVYRGFDELRGVEVAWNQATISDVLRTPDALHRMYAEVSLLADLRHDAIIA 98

Query: 72  FYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGHDPP 131
           F+ SW+   RRTFNFITE+F+SGTLR YR +Y  +   A+  WAR IL GL YLH     
Sbjct: 99  FHASWVHPSRRTFNFITELFSSGTLRSYRLRYPRVSRRAVAAWARAILRGLAYLHARG-- 158

Query: 132 VIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYNELV 191
           VIHRDLKCDN+F+NGHLGQVKIGDLGLA +L     A SVIGTPEFMAPE+YDE Y   V
Sbjct: 159 VIHRDLKCDNIFVNGHLGQVKIGDLGLAAVLRGCASARSVIGTPEFMAPEMYDECYGVGV 218

Query: 192 DVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCLENV 251
           DVYSFGMCM+EMLT EYPY+EC NPAQIYKKVT+GKLP+AFY + D +A+RF+ +CL + 
Sbjct: 219 DVYSFGMCMLEMLTNEYPYSECDNPAQIYKKVTAGKLPDAFYRLTDADARRFIGRCLVDA 278

Query: 252 SKRVSARELLLDPFLAPPNSNNASHN-----------EELFSSSLSPEKS------MSRR 311
           + R SA ELLLDPFL+ P+ N+  HN             L  S+ S E+        ++ 
Sbjct: 279 AHRPSAEELLLDPFLS-PSQNHDDHNIIAHATAPPPPLPLACSNSSEEQEEAAPAPAAKT 338

Query: 312 TDLAISGSINPKDDSIFLKVQIKVKNGKS-KNVYFAFDTLNDTAIDVATEMVKELEIISD 371
           TD+AI+G +N + D+IFLKVQI    G++ +N+YF FD  NDTA++VATEMVKEL+ I+D
Sbjct: 339 TDMAITGKLNKEHDTIFLKVQI--GGGRNVRNIYFPFDVANDTAMEVATEMVKELD-IAD 398

Query: 372 WDPLEIAVMIEKEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPH 431
            +P EIA MIE+EI  LVP          + Q  ++Y  D        NDDDD +   P+
Sbjct: 399 REPTEIAAMIEQEIVRLVPG---------YKQHEYSYADD--------NDDDDVS-GHPN 458

Query: 432 PFYYCGSSHGSSSDSLHAFYSFHEN--PNHHFGGTKDTSIATEWF-------QEDDTSSC 491
           PFYY  SS  SS  SL    S      P  H  G  D S    ++        +DD SS 
Sbjct: 459 PFYYLSSSPTSSQGSLCGVGSTSSEGFPGPH--GKVDWSRDYCYYPPSSVSVSDDDDSST 518

Query: 492 CSLNSFNYSDLSFYSNNEDEYEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDT 551
            SL+    + +S  S ++ +    ++     P   S ++             H  R +  
Sbjct: 519 SSLS----AAVSASSLHQQQQHCSASSSRLGPASASASEDGG--------GGHAGRPR-- 578

Query: 552 KIPNREIFESRSRSNNSPRLTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQ 587
               RE  E R R     R++R +SMV++RS+ LHR LVE L K+  FNTVGA+ +IG++
Sbjct: 579 ---QREGEEERRRR----RMSRNRSMVDMRSQLLHRTLVEELNKRLFFNTVGAVHDIGFR 593

BLAST of PI0020248 vs. ExPASy Swiss-Prot
Match: Q9STK6 (Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN=WNK3 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.1e-114
Identity = 208/382 (54.45%), Postives = 272/382 (71.20%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +++E+LGKGA K VY+ FD++ GIEVAWNQV L D F S E+L RLYSEVHLLK L H S
Sbjct: 23  RYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSSEDLDRLYSEVHLLKTLKHKS 82

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           II+FYTSWID    T N ITE+FTSG LR+YR+K++ +D+ A+K W+RQIL GLVYLH H
Sbjct: 83  IIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDLRALKKWSRQILEGLVYLHSH 142

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+FING+ G+VKIGDLGLA ILH ++ AHSVIGTPEFMAPELY+E+YN
Sbjct: 143 DPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSAHSVIGTPEFMAPELYEEDYN 202

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
            LVD+Y+FGMC++E++T EYPY+EC+N AQIY+KVTSG  P A   + D + + F+ KC+
Sbjct: 203 VLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIKPAALLNVTDPQVRAFIEKCI 262

Query: 249 ENVSKRVSARELLLDPFL---APPNSNNASHNEELFSSSLSPEKSMSRRTDLAISGSINP 308
             VS+R+SA+ELL DPFL        N +SH E  ++ +   +K       L        
Sbjct: 263 AKVSQRLSAKELLDDPFLKCYKENTENVSSHKENGYNGNGIVDKLSDSEVGLLTVEGQRK 322

Query: 309 KDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEK 368
             ++IFLK++I    G+ +N++F F+   DT+  VA EMV+EL++  D D   IA MI+ 
Sbjct: 323 DLNTIFLKLRITDSKGQIRNIHFPFNIETDTSFSVAIEMVEELDLTDDQDISTIAKMIDT 382

Query: 369 EISSLVPDREEWKLQKIHHQDS 388
           EI S +PD   W   ++   DS
Sbjct: 383 EIHSHIPD---WTPSRLIGDDS 401

BLAST of PI0020248 vs. ExPASy TrEMBL
Match: A0A0A0L051 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332110 PE=4 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 547/580 (94.31%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYT WIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTYWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPP+IHRDLKCDNVFINGHLGQVKIGDLGLA ILHDSQHAHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPIIHRDLKCDNVFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVYSFGMCMIEMLTLEYPY+EC NPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYSECFNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 258

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSINPKD 308
           ENVSKRVSARELLLDPFLAP N+NNASHNEEL SSSLSPEKS M+RRTDLAISGSINPKD
Sbjct: 259 ENVSKRVSARELLLDPFLAPSNANNASHNEELLSSSLSPEKSIMARRTDLAISGSINPKD 318

Query: 309 DSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 368
           DSIFLKVQIKVKNGKSKNVYFAFD LNDT IDVATEMVKELEIISDWDPLEIAVMIEKEI
Sbjct: 319 DSIFLKVQIKVKNGKSKNVYFAFDILNDTTIDVATEMVKELEIISDWDPLEIAVMIEKEI 378

Query: 369 SSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSD 428
           SSL+PD EEWKL KI HQDSFNYEQDH G +DN NDDDDENYATPHPFYYCGSSHGSSSD
Sbjct: 379 SSLIPDWEEWKLPKIQHQDSFNYEQDHDGDNDNDNDDDDENYATPHPFYYCGSSHGSSSD 438

Query: 429 SLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 488
           SLHAFYS  ENPNH+FGG KDTS  TEWF+EDDTSSCCSLNSFNYSDLSFYSNNEDEYEY
Sbjct: 439 SLHAFYSSRENPNHYFGGMKDTSNTTEWFREDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 498

Query: 489 DSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPRLTR 548
           DSNIKGREPQY+STTKQPTRFCPTMKIDSHHLRHKD K IPNRE+FESRSRSNNSPRLTR
Sbjct: 499 DSNIKGREPQYVSTTKQPTRFCPTMKIDSHHLRHKDNKIIPNREVFESRSRSNNSPRLTR 558

Query: 549 VKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           VKSMVNLRSETLHR LVEMLLKKRLFNTVGAMENIGYQKP
Sbjct: 559 VKSMVNLRSETLHRYLVEMLLKKRLFNTVGAMENIGYQKP 598

BLAST of PI0020248 vs. ExPASy TrEMBL
Match: A0A1S3CJ68 (probable serine/threonine-protein kinase WNK5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501457 PE=4 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 537/580 (92.59%), Postives = 556/580 (95.86%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQ+AHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 258

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSINPKD 308
           ENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSINPKD
Sbjct: 259 ENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSINPKD 318

Query: 309 DSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 368
           DSIFLKVQIKVKNGKSKNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI
Sbjct: 319 DSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 378

Query: 369 SSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSD 428
           SSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGSSSD
Sbjct: 379 SSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGSSSD 438

Query: 429 SLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 488
           SLH FY F ENPNHHFGG  DTSI TEWF+EDDTSSCCSLNSF+YSDLSFYSN+EDEYEY
Sbjct: 439 SLHDFYPFRENPNHHFGG-MDTSITTEWFREDDTSSCCSLNSFDYSDLSFYSNDEDEYEY 498

Query: 489 DSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPRLTR 548
           DSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR+TR
Sbjct: 499 DSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPRITR 558

Query: 549 VKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           VKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIG+QKP
Sbjct: 559 VKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGFQKP 597

BLAST of PI0020248 vs. ExPASy TrEMBL
Match: A0A1S3CIS3 (probable serine/threonine-protein kinase WNK5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501457 PE=4 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 4.8e-308
Identity = 537/583 (92.11%), Postives = 556/583 (95.37%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQ+AHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS---GKLPNAFYEIKDLEAQRFVR 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS   GKLPNAFYEIKDLEAQRFVR
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSKLQGKLPNAFYEIKDLEAQRFVR 258

Query: 249 KCLENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSIN 308
           KCLENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSIN
Sbjct: 259 KCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSIN 318

Query: 309 PKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIE 368
           PKDDSIFLKVQIKVKNGKSKNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIE
Sbjct: 319 PKDDSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIE 378

Query: 369 KEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGS 428
           KEISSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGS
Sbjct: 379 KEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGS 438

Query: 429 SSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDE 488
           SSDSLH FY F ENPNHHFGG  DTSI TEWF+EDDTSSCCSLNSF+YSDLSFYSN+EDE
Sbjct: 439 SSDSLHDFYPFRENPNHHFGG-MDTSITTEWFREDDTSSCCSLNSFDYSDLSFYSNDEDE 498

Query: 489 YEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPR 548
           YEYDSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR
Sbjct: 499 YEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPR 558

Query: 549 LTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           +TRVKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIG+QKP
Sbjct: 559 ITRVKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGFQKP 600

BLAST of PI0020248 vs. ExPASy TrEMBL
Match: A0A5A7SMB2 (Putative serine/threonine-protein kinase WNK5 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1220G00080 PE=4 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.5e-305
Identity = 532/580 (91.72%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQHAHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQHAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 258

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSINPKD 308
           ENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSINPKD
Sbjct: 259 ENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSINPKD 318

Query: 309 DSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 368
           DSIFLKVQI       KNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI
Sbjct: 319 DSIFLKVQI-------KNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 378

Query: 369 SSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSD 428
           SSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGSSSD
Sbjct: 379 SSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGSSSD 438

Query: 429 SLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 488
           SLH FY F ENPNHHFGG  DTSI TEWF+EDDTSSCCSLNSF+YSDLSFYSN+EDEYEY
Sbjct: 439 SLHDFYPFRENPNHHFGG-MDTSITTEWFREDDTSSCCSLNSFDYSDLSFYSNDEDEYEY 498

Query: 489 DSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPRLTR 548
           DSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR+TR
Sbjct: 499 DSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPRITR 558

Query: 549 VKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           VKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIGYQKP
Sbjct: 559 VKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGYQKP 590

BLAST of PI0020248 vs. ExPASy TrEMBL
Match: A0A1S3CKC9 (probable serine/threonine-protein kinase WNK5 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103501457 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.5e-290
Identity = 514/583 (88.16%), Postives = 531/583 (91.08%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQ+AHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS---GKLPNAFYEIKDLEAQRFVR 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS   GKLPNAFYEIKDLEAQRFVR
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSKLQGKLPNAFYEIKDLEAQRFVR 258

Query: 249 KCLENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSIN 308
           KCLENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSIN
Sbjct: 259 KCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSIN 318

Query: 309 PKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIE 368
           PKDDSIFLKVQIKVKNGKSKNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIE
Sbjct: 319 PKDDSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIE 378

Query: 369 KEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGS 428
           KEISSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGS
Sbjct: 379 KEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGS 438

Query: 429 SSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDE 488
           SSDSLH FY F ENPNHHFGG  DTSI TEWF+                         DE
Sbjct: 439 SSDSLHDFYPFRENPNHHFGG-MDTSITTEWFR-------------------------DE 498

Query: 489 YEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPR 548
           YEYDSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR
Sbjct: 499 YEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPR 558

Query: 549 LTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           +TRVKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIG+QKP
Sbjct: 559 ITRVKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGFQKP 575

BLAST of PI0020248 vs. NCBI nr
Match: XP_004142734.2 (probable serine/threonine-protein kinase WNK5 [Cucumis sativus] >KGN54444.1 hypothetical protein Csa_012775 [Cucumis sativus])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 547/580 (94.31%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYT WIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTYWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPP+IHRDLKCDNVFINGHLGQVKIGDLGLA ILHDSQHAHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPIIHRDLKCDNVFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVYSFGMCMIEMLTLEYPY+EC NPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYSECFNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 258

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSINPKD 308
           ENVSKRVSARELLLDPFLAP N+NNASHNEEL SSSLSPEKS M+RRTDLAISGSINPKD
Sbjct: 259 ENVSKRVSARELLLDPFLAPSNANNASHNEELLSSSLSPEKSIMARRTDLAISGSINPKD 318

Query: 309 DSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 368
           DSIFLKVQIKVKNGKSKNVYFAFD LNDT IDVATEMVKELEIISDWDPLEIAVMIEKEI
Sbjct: 319 DSIFLKVQIKVKNGKSKNVYFAFDILNDTTIDVATEMVKELEIISDWDPLEIAVMIEKEI 378

Query: 369 SSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSD 428
           SSL+PD EEWKL KI HQDSFNYEQDH G +DN NDDDDENYATPHPFYYCGSSHGSSSD
Sbjct: 379 SSLIPDWEEWKLPKIQHQDSFNYEQDHDGDNDNDNDDDDENYATPHPFYYCGSSHGSSSD 438

Query: 429 SLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 488
           SLHAFYS  ENPNH+FGG KDTS  TEWF+EDDTSSCCSLNSFNYSDLSFYSNNEDEYEY
Sbjct: 439 SLHAFYSSRENPNHYFGGMKDTSNTTEWFREDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 498

Query: 489 DSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPRLTR 548
           DSNIKGREPQY+STTKQPTRFCPTMKIDSHHLRHKD K IPNRE+FESRSRSNNSPRLTR
Sbjct: 499 DSNIKGREPQYVSTTKQPTRFCPTMKIDSHHLRHKDNKIIPNREVFESRSRSNNSPRLTR 558

Query: 549 VKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           VKSMVNLRSETLHR LVEMLLKKRLFNTVGAMENIGYQKP
Sbjct: 559 VKSMVNLRSETLHRYLVEMLLKKRLFNTVGAMENIGYQKP 598

BLAST of PI0020248 vs. NCBI nr
Match: XP_008463254.1 (PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X2 [Cucumis melo])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.3e-309
Identity = 537/580 (92.59%), Postives = 556/580 (95.86%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQ+AHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 258

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSINPKD 308
           ENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSINPKD
Sbjct: 259 ENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSINPKD 318

Query: 309 DSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 368
           DSIFLKVQIKVKNGKSKNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI
Sbjct: 319 DSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 378

Query: 369 SSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSD 428
           SSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGSSSD
Sbjct: 379 SSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGSSSD 438

Query: 429 SLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 488
           SLH FY F ENPNHHFGG  DTSI TEWF+EDDTSSCCSLNSF+YSDLSFYSN+EDEYEY
Sbjct: 439 SLHDFYPFRENPNHHFGG-MDTSITTEWFREDDTSSCCSLNSFDYSDLSFYSNDEDEYEY 498

Query: 489 DSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPRLTR 548
           DSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR+TR
Sbjct: 499 DSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPRITR 558

Query: 549 VKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           VKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIG+QKP
Sbjct: 559 VKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGFQKP 597

BLAST of PI0020248 vs. NCBI nr
Match: XP_008463253.1 (PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X1 [Cucumis melo])

HSP 1 Score: 1066.2 bits (2756), Expect = 9.9e-308
Identity = 537/583 (92.11%), Postives = 556/583 (95.37%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQ+AHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS---GKLPNAFYEIKDLEAQRFVR 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS   GKLPNAFYEIKDLEAQRFVR
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSKLQGKLPNAFYEIKDLEAQRFVR 258

Query: 249 KCLENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSIN 308
           KCLENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSIN
Sbjct: 259 KCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSIN 318

Query: 309 PKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIE 368
           PKDDSIFLKVQIKVKNGKSKNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIE
Sbjct: 319 PKDDSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIE 378

Query: 369 KEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGS 428
           KEISSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGS
Sbjct: 379 KEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGS 438

Query: 429 SSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDE 488
           SSDSLH FY F ENPNHHFGG  DTSI TEWF+EDDTSSCCSLNSF+YSDLSFYSN+EDE
Sbjct: 439 SSDSLHDFYPFRENPNHHFGG-MDTSITTEWFREDDTSSCCSLNSFDYSDLSFYSNDEDE 498

Query: 489 YEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPR 548
           YEYDSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR
Sbjct: 499 YEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPR 558

Query: 549 LTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           +TRVKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIG+QKP
Sbjct: 559 ITRVKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGFQKP 600

BLAST of PI0020248 vs. NCBI nr
Match: KAA0025381.1 (putative serine/threonine-protein kinase WNK5 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.7e-304
Identity = 532/580 (91.72%), Postives = 549/580 (94.66%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQHAHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQHAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 258

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSINPKD 308
           ENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSINPKD
Sbjct: 259 ENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSINPKD 318

Query: 309 DSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 368
           DSIFLKVQI       KNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI
Sbjct: 319 DSIFLKVQI-------KNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIEKEI 378

Query: 369 SSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGSSSD 428
           SSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGSSSD
Sbjct: 379 SSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGSSSD 438

Query: 429 SLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDEYEY 488
           SLH FY F ENPNHHFGG  DTSI TEWF+EDDTSSCCSLNSF+YSDLSFYSN+EDEYEY
Sbjct: 439 SLHDFYPFRENPNHHFGG-MDTSITTEWFREDDTSSCCSLNSFDYSDLSFYSNDEDEYEY 498

Query: 489 DSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPRLTR 548
           DSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR+TR
Sbjct: 499 DSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPRITR 558

Query: 549 VKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           VKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIGYQKP
Sbjct: 559 VKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGYQKP 590

BLAST of PI0020248 vs. NCBI nr
Match: XP_008463255.1 (PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X3 [Cucumis melo])

HSP 1 Score: 1006.9 bits (2602), Expect = 7.1e-290
Identity = 514/583 (88.16%), Postives = 531/583 (91.08%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEEL+RLYSEVHLLKNLNH+S
Sbjct: 19  RFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELERLYSEVHLLKNLNHNS 78

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKY+NIDIEAIKNWARQILHGLVYLHGH
Sbjct: 79  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEAIKNWARQILHGLVYLHGH 138

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILH SQ+AHSVIGTPEFMAPELYDEEYN
Sbjct: 139 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHSVIGTPEFMAPELYDEEYN 198

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS---GKLPNAFYEIKDLEAQRFVR 248
           ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTS   GKLPNAFYEIKDLEAQRFVR
Sbjct: 199 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSKLQGKLPNAFYEIKDLEAQRFVR 258

Query: 249 KCLENVSKRVSARELLLDPFLAPPNSNNASHNEELFSSSLSPEKS-MSRRTDLAISGSIN 308
           KCLENVSKRVSARELLL+PFLAP NSNN SHNEEL SSSLSPEKS M+RRTDLAISGSIN
Sbjct: 259 KCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKSIMARRTDLAISGSIN 318

Query: 309 PKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIE 368
           PKDDSIFLKVQIKVKNGKSKNVYFAFD LNDTAIDVATEMVKELEIISDWDPLEIAVMIE
Sbjct: 319 PKDDSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKELEIISDWDPLEIAVMIE 378

Query: 369 KEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGSSHGS 428
           KEISSL+PD EEWK  +IHHQDSFNYEQDH+G DDN +DDDDENYATPHPFYYCGSSHGS
Sbjct: 379 KEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDENYATPHPFYYCGSSHGS 438

Query: 429 SSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQEDDTSSCCSLNSFNYSDLSFYSNNEDE 488
           SSDSLH FY F ENPNHHFGG  DTSI TEWF+                         DE
Sbjct: 439 SSDSLHDFYPFRENPNHHFGG-MDTSITTEWFR-------------------------DE 498

Query: 489 YEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTK-IPNREIFESRSRSNNSPR 548
           YEYDSNIKGRE +YISTTKQPTRFCPTMKIDSHHLRHKDT+ IPNRE FE+ S SNNSPR
Sbjct: 499 YEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKDTRIIPNRETFENGSLSNNSPR 558

Query: 549 LTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           +TRVKSMVNLRSETLHR LVE LLKKRLFNTVGAMENIG+QKP
Sbjct: 559 ITRVKSMVNLRSETLHRYLVETLLKKRLFNTVGAMENIGFQKP 575

BLAST of PI0020248 vs. TAIR 10
Match: AT5G58350.1 (with no lysine (K) kinase 4 )

HSP 1 Score: 528.1 bits (1359), Expect = 9.0e-150
Identity = 305/589 (51.78%), Postives = 387/589 (65.70%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +F EILG+GA KTVYK  DE LGIEVAW+QV LK+V  S  +LQRLYSEVHLL  LNH S
Sbjct: 20  RFAEILGRGAMKTVYKAIDEKLGIEVAWSQVKLKEVLRSSVDLQRLYSEVHLLSTLNHKS 79

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIRFYTSWID H  T NFITE+FTSGTLR+Y+ KY  IDI AIK+WARQIL GLVYLH H
Sbjct: 80  IIRFYTSWIDVHNHTLNFITELFTSGTLRQYKNKYLRIDIRAIKSWARQILEGLVYLHEH 139

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+F+NGHLGQVKIGDLGLA +L D   AHS+IGTPEFMAPELY+E YN
Sbjct: 140 DPPVIHRDLKCDNIFVNGHLGQVKIGDLGLARMLRDCHSAHSIIGTPEFMAPELYEENYN 199

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           EL+DVYSFGMC +EM+T E+PY+EC++PAQIYKKV  GKLP AFY + D+EAQRF+ KCL
Sbjct: 200 ELIDVYSFGMCFLEMITSEFPYSECNHPAQIYKKVVGGKLPGAFYRVGDIEAQRFIGKCL 259

Query: 249 ENVSKRVSARELLLDPFLAPPNS----NNASHNEELFSSSLSPE----KSMSRRTDLAIS 308
            + SKRVSA+ELL DPFLA   S     + + N + F +    +    +    RT+++I+
Sbjct: 260 VSASKRVSAKELLQDPFLASDESWMVYTSGAGNPKPFLNENEMDTLKLEDDELRTEMSIA 319

Query: 309 GSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIA 368
           G +  +D+ I L+VQI   NG + NV+F FD +NDT+IDVA EMVKELEII DW+P+EIA
Sbjct: 320 GKLGAEDNKIDLEVQIAYDNGLANNVFFPFDIMNDTSIDVAKEMVKELEII-DWEPVEIA 379

Query: 369 VMIEKEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGDDNYNDDDDENYATPHPFYYCGS 428
            MI+  ISSLV D   WK           YE+D     D++    D         ++  S
Sbjct: 380 KMIDGAISSLVSD---WK-----------YEEDDETPHDHHRHRTDS--------FHSSS 439

Query: 429 SHGSSSDSLHAFYSFHENPNHHFGGTKDTSIATEWFQE---DDTSSCCSLNSFNYSDLSF 488
           SH SSS +           N+   G +D      W Q+   D+T S  S +S +YS+L++
Sbjct: 440 SHASSSQA--------SLSNYMARGLQD------WVQDDLHDETYSQSSSHSGSYSNLNY 499

Query: 489 YSNNEDEYEYDSNIKGREPQYISTTKQPTRFCPTMKIDSHHLRHKDTKIPNREIFESRSR 548
            +   DEY   S +       +S T   TRFCP    +S HL+             +RS 
Sbjct: 500 IA--VDEYSSQSPV-------MSRTHNMTRFCPE---ESSHLQSGQANAYAASSSTNRSL 559

Query: 549 SNNSPRLTRVKSMVNLRSETLHRCLVEMLLKKRLFNTVGAMENIGYQKP 587
           ++++  LTR +S+V+++ + LHR   E   K+RLF TVG +E +G+Q P
Sbjct: 560 ASDNRTLTRNRSLVDVQRQLLHRSPGEEARKRRLFKTVGDVETVGFQSP 559

BLAST of PI0020248 vs. TAIR 10
Match: AT3G51630.1 (with no lysine (K) kinase 5 )

HSP 1 Score: 516.9 bits (1330), Expect = 2.1e-146
Identity = 264/406 (65.02%), Postives = 324/406 (79.80%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FRE+LGKGA KTVYK FD+VLG+EVAWNQV L +VF SPE LQRLYSEVHLLKNLNH+S
Sbjct: 26  RFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLYSEVHLLKNLNHES 85

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIR+ TSWID +RRTFNFITE+FTSGTLREYR+KY+ +DI AIK+WARQIL+GL YLHGH
Sbjct: 86  IIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWARQILNGLAYLHGH 145

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+F+NGHLGQVKIGDLGLA IL  SQ+AHSVIGTPEFMAPELY+E+YN
Sbjct: 146 DPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTPEFMAPELYEEDYN 205

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVD+YSFGMC++EMLT EYPY+EC+NPAQIYKKVTSGKLP++F+ I+  EAQRFV KCL
Sbjct: 206 ELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLIQHTEAQRFVGKCL 265

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASH----NEELFSSSLSPEKSM----------SRR 308
           E VS+R+ A+ELL DPFLA  +  + +      ++L   +L+   ++          +R 
Sbjct: 266 ETVSRRLPAKELLADPFLAATDERDLAPLFRLPQQLAIQNLAANGTVVEHLPSTTDPTRT 325

Query: 309 TDLAISGSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDW 368
           TD++I+G +N +D +IFL+VQI   +G  +N+ F F+ L+DT ++VA EMVKELE I+DW
Sbjct: 326 TDMSITGKMNSEDHTIFLQVQILDGDGHMRNIQFPFNILSDTPLEVALEMVKELE-ITDW 385

Query: 369 DPLEIAVMIEKEISSLVPDREEWKL--QKIHHQDSFNYEQDHHGGD 399
           DPLEIA MIE EIS LVP+   W+     I H +SF +E D   GD
Sbjct: 386 DPLEIAAMIENEISLLVPN---WRANDSSIRH-ESFGHEDDEDNGD 426

BLAST of PI0020248 vs. TAIR 10
Match: AT3G51630.2 (with no lysine (K) kinase 5 )

HSP 1 Score: 516.9 bits (1330), Expect = 2.1e-146
Identity = 264/406 (65.02%), Postives = 324/406 (79.80%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +FRE+LGKGA KTVYK FD+VLG+EVAWNQV L +VF SPE LQRLYSEVHLLKNLNH+S
Sbjct: 26  RFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLYSEVHLLKNLNHES 85

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           IIR+ TSWID +RRTFNFITE+FTSGTLREYR+KY+ +DI AIK+WARQIL+GL YLHGH
Sbjct: 86  IIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWARQILNGLAYLHGH 145

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+F+NGHLGQVKIGDLGLA IL  SQ+AHSVIGTPEFMAPELY+E+YN
Sbjct: 146 DPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTPEFMAPELYEEDYN 205

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVD+YSFGMC++EMLT EYPY+EC+NPAQIYKKVTSGKLP++F+ I+  EAQRFV KCL
Sbjct: 206 ELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLIQHTEAQRFVGKCL 265

Query: 249 ENVSKRVSARELLLDPFLAPPNSNNASH----NEELFSSSLSPEKSM----------SRR 308
           E VS+R+ A+ELL DPFLA  +  + +      ++L   +L+   ++          +R 
Sbjct: 266 ETVSRRLPAKELLADPFLAATDERDLAPLFRLPQQLAIQNLAANGTVVEHLPSTTDPTRT 325

Query: 309 TDLAISGSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDW 368
           TD++I+G +N +D +IFL+VQI   +G  +N+ F F+ L+DT ++VA EMVKELE I+DW
Sbjct: 326 TDMSITGKMNSEDHTIFLQVQILDGDGHMRNIQFPFNILSDTPLEVALEMVKELE-ITDW 385

Query: 369 DPLEIAVMIEKEISSLVPDREEWKL--QKIHHQDSFNYEQDHHGGD 399
           DPLEIA MIE EIS LVP+   W+     I H +SF +E D   GD
Sbjct: 386 DPLEIAAMIENEISLLVPN---WRANDSSIRH-ESFGHEDDEDNGD 426

BLAST of PI0020248 vs. TAIR 10
Match: AT3G48260.1 (with no lysine (K) kinase 3 )

HSP 1 Score: 414.5 bits (1064), Expect = 1.5e-115
Identity = 208/382 (54.45%), Postives = 272/382 (71.20%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           +++E+LGKGA K VY+ FD++ GIEVAWNQV L D F S E+L RLYSEVHLLK L H S
Sbjct: 23  RYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSSEDLDRLYSEVHLLKTLKHKS 82

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           II+FYTSWID    T N ITE+FTSG LR+YR+K++ +D+ A+K W+RQIL GLVYLH H
Sbjct: 83  IIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDLRALKKWSRQILEGLVYLHSH 142

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
           DPPVIHRDLKCDN+FING+ G+VKIGDLGLA ILH ++ AHSVIGTPEFMAPELY+E+YN
Sbjct: 143 DPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSAHSVIGTPEFMAPELYEEDYN 202

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
            LVD+Y+FGMC++E++T EYPY+EC+N AQIY+KVTSG  P A   + D + + F+ KC+
Sbjct: 203 VLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIKPAALLNVTDPQVRAFIEKCI 262

Query: 249 ENVSKRVSARELLLDPFL---APPNSNNASHNEELFSSSLSPEKSMSRRTDLAISGSINP 308
             VS+R+SA+ELL DPFL        N +SH E  ++ +   +K       L        
Sbjct: 263 AKVSQRLSAKELLDDPFLKCYKENTENVSSHKENGYNGNGIVDKLSDSEVGLLTVEGQRK 322

Query: 309 KDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAIDVATEMVKELEIISDWDPLEIAVMIEK 368
             ++IFLK++I    G+ +N++F F+   DT+  VA EMV+EL++  D D   IA MI+ 
Sbjct: 323 DLNTIFLKLRITDSKGQIRNIHFPFNIETDTSFSVAIEMVEELDLTDDQDISTIAKMIDT 382

Query: 369 EISSLVPDREEWKLQKIHHQDS 388
           EI S +PD   W   ++   DS
Sbjct: 383 EIHSHIPD---WTPSRLIGDDS 401

BLAST of PI0020248 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 407.5 bits (1046), Expect = 1.8e-113
Identity = 225/477 (47.17%), Postives = 311/477 (65.20%), Query Frame = 0

Query: 9   QFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEELQRLYSEVHLLKNLNHDS 68
           ++ EILGKGA+KTVY+ FDE  GIEVAWNQV L++   +PEEL++ + E+HLLK LNH +
Sbjct: 25  RYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEKFFREIHLLKTLNHQN 84

Query: 69  IIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEAIKNWARQILHGLVYLHGH 128
           I++FYTSW+DT+  + NF+TE+FTSGTLR+YR ++R ++I A+K W +QIL GL+YLH  
Sbjct: 85  IMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQWCKQILKGLLYLHSR 144

Query: 129 DPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPELYDEEYN 188
            PP+IHRDLKCDN+FING+ G+VKIGDLGLA IL  S HA   +GTPEFMAPE+YDEEYN
Sbjct: 145 SPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKS-HAVRCVGTPEFMAPEVYDEEYN 204

Query: 189 ELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPNAFYEIKDLEAQRFVRKCL 248
           ELVDVY+FGMC++EM+T +YPY+EC++PAQIYKKVTSGK P AFY +KD E + FV KCL
Sbjct: 205 ELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCL 264

Query: 249 ENVSKRVSARELLLDPFLAPPNS--------------------------NNASHNEELFS 308
            NV+ R++A ELL DPFL   N                           ++  ++++  S
Sbjct: 265 ANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRHPLIDDPLYHDQFES 324

Query: 309 SSLSPEKSMSR----RTDLAISGSINPKDDSIFLKVQIKVKNGKSKNVYFAFDTLNDTAI 368
           S +      +       D++I G  N  DD IFL+++I    G+ +N+YF F+T  DTA 
Sbjct: 325 SQICEIDLFANDDEDHVDISIKGKRN-GDDGIFLRLRISDAEGRIRNIYFPFETAIDTAW 384

Query: 369 DVATEMVKELEIISDWDPLEIAVMIEKEISSLVPDREEWKLQKIHHQDSFNYEQDHHGGD 428
            VA EMV EL+ I++ D  +IA MI+ EI++LVPD   WK              +     
Sbjct: 385 SVAVEMVSELD-ITNQDVAKIAEMIDAEIAALVPD---WK--------------NDTESS 444

Query: 429 DNYNDDDDENYATPHPFYYCG--SSHGSSSDSLHAFYSFHENPNHHFGGTKDTSIAT 454
            N N++ + N A      +CG  +S+G   +++ +    H N +H F  ++D S ++
Sbjct: 445 QNVNNNKNNNTAG-----FCGECASNGYIQETVSSGEKSHHN-HHEFDSSEDKSCSS 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVL51.3e-14851.78Probable serine/threonine-protein kinase WNK4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SCU52.9e-14565.02Probable serine/threonine-protein kinase WNK5 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q2QYL81.9e-12846.90Probable serine/threonine-protein kinase WNK8 OS=Oryza sativa subsp. japonica OX... [more]
Q2RBE31.4e-12647.67Probable serine/threonine-protein kinase WNK7 OS=Oryza sativa subsp. japonica OX... [more]
Q9STK62.1e-11454.45Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0L0510.0e+0094.31Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332... [more]
A0A1S3CJ681.1e-30992.59probable serine/threonine-protein kinase WNK5 isoform X2 OS=Cucumis melo OX=3656... [more]
A0A1S3CIS34.8e-30892.11probable serine/threonine-protein kinase WNK5 isoform X1 OS=Cucumis melo OX=3656... [more]
A0A5A7SMB28.5e-30591.72Putative serine/threonine-protein kinase WNK5 isoform X2 OS=Cucumis melo var. ma... [more]
A0A1S3CKC93.5e-29088.16probable serine/threonine-protein kinase WNK5 isoform X3 OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
XP_004142734.20.0e+0094.31probable serine/threonine-protein kinase WNK5 [Cucumis sativus] >KGN54444.1 hypo... [more]
XP_008463254.12.3e-30992.59PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X2 [Cucumis mel... [more]
XP_008463253.19.9e-30892.11PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X1 [Cucumis mel... [more]
KAA0025381.11.7e-30491.72putative serine/threonine-protein kinase WNK5 isoform X2 [Cucumis melo var. maku... [more]
XP_008463255.17.1e-29088.16PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X3 [Cucumis mel... [more]
Match NameE-valueIdentityDescription
AT5G58350.19.0e-15051.78with no lysine (K) kinase 4 [more]
AT3G51630.12.1e-14665.02with no lysine (K) kinase 5 [more]
AT3G51630.22.1e-14665.02with no lysine (K) kinase 5 [more]
AT3G48260.11.5e-11554.45with no lysine (K) kinase 3 [more]
AT3G22420.11.8e-11347.17with no lysine (K) kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 8..266
e-value: 1.5E-59
score: 213.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 10..266
e-value: 3.2E-48
score: 164.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 8..266
score: 39.990044
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 7..89
e-value: 5.0E-26
score: 92.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 91..270
e-value: 9.5E-49
score: 167.4
NoneNo IPR availableGENE3D3.10.20.90coord: 308..378
e-value: 1.1E-5
score: 27.2
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 9..586
NoneNo IPR availablePANTHERPTHR13902:SF68SERINE/THREONINE-PROTEIN KINASE WNK5-RELATEDcoord: 9..586
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 10..266
e-value: 9.91132E-154
score: 439.739
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 132..144
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 10..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0020248.1PI0020248.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0048573 photoperiodism, flowering
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0043424 protein histidine kinase binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity