PI0020172 (gene) Melon (PI 482460) v1

Overview


Name	PI0020172
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Retrovirus-related Pol polyprotein from transposon 17.6
Location	chr01: 15672806 .. 15673060 (+)
RNA-Seq Expression	PI0020172
Synteny	PI0020172

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGACGCAAGTGATGTGGCAGTAGGGGATATGCTAGGTCGAAAGAAAGATAAAGTGATCCACCTTATATACTACGCGAGCAAGACTCTCACAAATGTTCAAGAGAATTATACCACCACAGAGAAGGAATTGCTCGCGGTAGTATTTGCGATTGAAAAGTTCAGAAGTTACATTGTTGGCTCTAAAGTTATGTTACATTTTGATCCCTCTACGATCAAATTCCTCATGGCAAAGAAGGATGCAAAACCGTAG

mRNA sequence

ATGTGTGACGCAAGTGATGTGGCAGTAGGGGATATGCTAGGTCGAAAGAAAGATAAAGTGATCCACCTTATATACTACGCGAGCAAGACTCTCACAAATGTTCAAGAGAATTATACCACCACAGAGAAGGAATTGCTCGCGGTAGTATTTGCGATTGAAAAGTTCAGAAGTTACATTGTTGGCTCTAAAGTTATGTTACATTTTGATCCCTCTACGATCAAATTCCTCATGGCAAAGAAGGATGCAAAACCGTAG

Coding sequence (CDS)

ATGTGTGACGCAAGTGATGTGGCAGTAGGGGATATGCTAGGTCGAAAGAAAGATAAAGTGATCCACCTTATATACTACGCGAGCAAGACTCTCACAAATGTTCAAGAGAATTATACCACCACAGAGAAGGAATTGCTCGCGGTAGTATTTGCGATTGAAAAGTTCAGAAGTTACATTGTTGGCTCTAAAGTTATGTTACATTTTGATCCCTCTACGATCAAATTCCTCATGGCAAAGAAGGATGCAAAACCGTAG

Protein sequence

MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIVGSKVMLHFDPSTIKFLMAKKDAKP

Homology

[Close]

BLAST of PI0020172 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-07
Identity = 32/79 (40.51%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 3   DASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIVGS 62
           DASDVA+G +L +      H + Y S+TL   + NY+T EKELLA+V+A + FR Y++G 
Sbjct: 514 DASDVALGAVLSQDG----HPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGR 573

Query: 63  KVMLHFDPSTIKFLMAKKD 82
              +  D   + +L   KD
Sbjct: 574 HFEISSDHQPLSWLYRMKD 588

[Close]

BLAST of PI0020172 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.0e-06
Identity = 28/79 (35.44%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 3   DASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIVGS 62
           DAS++A+G +L +      H I + S+TL + + NY+  EKELLA+V+A + FR Y++G 
Sbjct: 513 DASNLALGAVLSQNG----HPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGR 572

Query: 63  KVMLHFDPSTIKFLMAKKD 82
           + ++  D   +++L   K+
Sbjct: 573 QFLIASDHQPLRWLHNLKE 587

[Close]

BLAST of PI0020172 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.1e-05
Identity = 24/80 (30.00%), Postives = 48/80 (60.00%), Query Frame = 0


Query: 3   DASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIVGS 62
           DAS+ A+G +L +        I Y S++L   +ENY T EKE+LA++++++  R+Y+ G+
Sbjct: 440 DASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGA 499

Query: 63  -KVMLHFDPSTIKFLMAKKD 82
             + ++ D   + F +  ++
Sbjct: 500 GTIKVYTDHQPLTFALGNRN 519

[Close]

BLAST of PI0020172 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.6e-04
Identity = 24/59 (40.68%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 3   DASDVAVGDMLGRK--KDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYI 60
           DASDVAVG +L +K   DK   + YY++K ++  Q NY+ ++KE+LA++ +++ +R Y+
Sbjct: 713 DASDVAVGAVLSQKHDDDKYYPVGYYSAK-MSKAQLNYSVSDKEMLAIIKSLKHWRHYL 770

[Close]

BLAST of PI0020172 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.6e-04
Identity = 24/59 (40.68%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 3   DASDVAVGDMLGRK--KDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYI 60
           DASDVAVG +L +K   DK   + YY++K ++  Q NY+ ++KE+LA++ +++ +R Y+
Sbjct: 713 DASDVAVGAVLSQKHDDDKYYPVGYYSAK-MSKAQLNYSVSDKEMLAIIKSLKHWRHYL 770

[Close]

BLAST of PI0020172 vs. ExPASy TrEMBL
Match: A0A5D3DS34 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001700 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-25
Identity = 63/83 (75.90%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASDV VGDM G+KKDKV   IYYA KTL   +ENYTT EKELLA+VFAIEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVMLHFDPSTIKFLMAKKDAK 84
           GSKV +H D S I++LMAKKDAK
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAK 160

[Close]

BLAST of PI0020172 vs. ExPASy TrEMBL
Match: A0A5A7V586 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002210 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-25
Identity = 63/83 (75.90%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASDV VGDM G+KKDKV   IYYA KTL   +ENYTT EKELLA+VFAIEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVMLHFDPSTIKFLMAKKDAK 84
           GSKV +H D S I++LMAKKDAK
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAK 134

[Close]

BLAST of PI0020172 vs. ExPASy TrEMBL
Match: A0A087GID7 (Reverse transcriptase OS=Arabis alpina OX=50452 GN=AALP_AA7G159800 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.0e-25
Identity = 58/84 (69.05%), Postives = 70/84 (83.33%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASD AVG +LG+KKDK +H IYYAS+TL   Q+NY TTEKELLA+VFA EKFR Y++
Sbjct: 648 MCDASDFAVGAVLGQKKDKKLHAIYYASRTLDEAQQNYATTEKELLALVFAFEKFRPYLI 707

Query: 61  GSKVMLHFDPSTIKFLMAKKDAKP 85
           GSKV++H D S +K+LM KKDAKP
Sbjct: 708 GSKVIVHTDHSALKYLMQKKDAKP 731

[Close]

BLAST of PI0020172 vs. ExPASy TrEMBL
Match: A0A6J1DHH9 (LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 OS=Momordica charantia OX=3673 GN=LOC111020110 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.5e-25
Identity = 57/84 (67.86%), Postives = 72/84 (85.71%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASD A+G MLG++K+K++H +YYASKTLT  Q NYTTTEKELLAVVFA +KFRSY++
Sbjct: 352 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAVVFAFDKFRSYLI 411

Query: 61  GSKVMLHFDPSTIKFLMAKKDAKP 85
           G+KV++  D S +K+L AKKDAKP
Sbjct: 412 GTKVIVFTDHSALKYLFAKKDAKP 435

[Close]

BLAST of PI0020172 vs. ExPASy TrEMBL
Match: A0A087FZ25 (Integrase catalytic domain-containing protein (Fragment) OS=Arabis alpina OX=50452 GN=AALP_AAs74748U000100 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.5e-25
Identity = 58/84 (69.05%), Postives = 70/84 (83.33%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASD AVG +LG+KKDK +H IYYAS+TL + Q+NY TTEKELL VVFA EKFR Y++
Sbjct: 383 MCDASDFAVGAVLGQKKDKKLHAIYYASRTLDDAQKNYATTEKELLPVVFAFEKFRPYLI 442

Query: 61  GSKVMLHFDPSTIKFLMAKKDAKP 85
           GSKV++H D S +K+LM KKDAKP
Sbjct: 443 GSKVIVHTDHSALKYLMQKKDAKP 466

[Close]

BLAST of PI0020172 vs. NCBI nr
Match: KAA0062414.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 125.2 bits (313), Expect = 2.7e-25
Identity = 63/83 (75.90%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASDV VGDM G+KKDKV   IYYA KTL   +ENYTT EKELLA+VFAIEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVMLHFDPSTIKFLMAKKDAK 84
           GSKV +H D S I++LMAKKDAK
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAK 134

[Close]

BLAST of PI0020172 vs. NCBI nr
Match: TYK26567.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 125.2 bits (313), Expect = 2.7e-25
Identity = 63/83 (75.90%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASDV VGDM G+KKDKV   IYYA KTL   +ENYTT EKELLA+VFAIEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVMLHFDPSTIKFLMAKKDAK 84
           GSKV +H D S I++LMAKKDAK
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAK 160

[Close]

BLAST of PI0020172 vs. NCBI nr
Match: XP_010507141.1 (PREDICTED: uncharacterized protein LOC104783719 [Camelina sativa])

HSP 1 Score: 124.4 bits (311), Expect = 4.6e-25
Identity = 59/84 (70.24%), Postives = 72/84 (85.71%), Query Frame = 0


Query: 1  MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
          MCDASD AVG +LG++KDK +H+IYYAS+TL + Q NY TTEKELLAVVFA EKFRSY+V
Sbjct: 1  MCDASDFAVGAVLGQRKDKKLHVIYYASRTLDDAQRNYETTEKELLAVVFAFEKFRSYLV 60

Query: 61 GSKVMLHFDPSTIKFLMAKKDAKP 85
          GSKV++H D + +K+LM KKDAKP
Sbjct: 61 GSKVIVHTDHAALKYLMQKKDAKP 84

[Close]

BLAST of PI0020172 vs. NCBI nr
Match: XP_019100932.1 (PREDICTED: uncharacterized protein LOC104789643 [Camelina sativa])

HSP 1 Score: 124.0 bits (310), Expect = 6.1e-25
Identity = 59/84 (70.24%), Postives = 70/84 (83.33%), Query Frame = 0


Query: 1    MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
            MCDASD AVG MLG++KDK +H IYYAS+TL   Q NY TTEKELLAVVFA EKFRSY+V
Sbjct: 973  MCDASDFAVGAMLGQRKDKKLHAIYYASRTLDEAQRNYATTEKELLAVVFAFEKFRSYLV 1032

Query: 61   GSKVMLHFDPSTIKFLMAKKDAKP 85
            GSKV++H D + +++LM KKDAKP
Sbjct: 1033 GSKVIVHTDHAALRYLMQKKDAKP 1056

[Close]

BLAST of PI0020172 vs. NCBI nr
Match: XP_024015893.1 (uncharacterized protein LOC112089147 [Eutrema salsugineum])

HSP 1 Score: 123.2 bits (308), Expect = 1.0e-24
Identity = 59/84 (70.24%), Postives = 70/84 (83.33%), Query Frame = 0


Query: 1   MCDASDVAVGDMLGRKKDKVIHLIYYASKTLTNVQENYTTTEKELLAVVFAIEKFRSYIV 60
           MCDASD AVG +LG+KKDK +H IYYAS+TL + Q NY TTEKELLAVVFA EKFR Y+V
Sbjct: 267 MCDASDFAVGAVLGQKKDKKLHAIYYASRTLDDAQRNYATTEKELLAVVFAFEKFRPYLV 326

Query: 61  GSKVMLHFDPSTIKFLMAKKDAKP 85
           GSKV++H D S++K+ M KKDAKP
Sbjct: 327 GSKVIVHTDHSSLKYFMQKKDAKP 350

The following BLAST results are available for this feature:

BLAST of PI0020172 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P04323	1.6e-07	40.51	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P20825	5.0e-06	35.44	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
Q8I7P9	1.1e-05	30.00	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
Q9UR07	3.6e-04	40.68	Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT41	3.6e-04	40.68	Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]

BLAST of PI0020172 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DS34	1.3e-25	75.90	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7V586	1.3e-25	75.90	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A087GID7	5.0e-25	69.05	Reverse transcriptase OS=Arabis alpina OX=50452 GN=AALP_AA7G159800 PE=4 SV=1	[more]
A0A6J1DHH9	6.5e-25	67.86	LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 OS=Momordica charantia...	[more]
A0A087FZ25	8.5e-25	69.05	Integrase catalytic domain-containing protein (Fragment) OS=Arabis alpina OX=504...	[more]

BLAST of PI0020172 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0062414.1	2.7e-25	75.90	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
TYK26567.1	2.7e-25	75.90	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
XP_010507141.1	4.6e-25	70.24	PREDICTED: uncharacterized protein LOC104783719 [Camelina sativa]	[more]
XP_019100932.1	6.1e-25	70.24	PREDICTED: uncharacterized protein LOC104789643 [Camelina sativa]	[more]
XP_024015893.1	1.0e-24	70.24	uncharacterized protein LOC112089147 [Eutrema salsugineum]	[more]

BLAST of PI0020172 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 2..81 e-value: 2.0E-21 score: 76.3
None	No IPR available	GENE3D	3.10.20.370		coord: 1..66 e-value: 3.9E-6 score: 28.8
None	No IPR available	CDD	cd09274	RNase_HI_RT_Ty3	coord: 1..84 e-value: 1.69591E-30 score: 102.186
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0020172.1	PI0020172.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding