PI0019573 (gene) Melon (PI 482460) v1

Overview


Name	PI0019573
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Serine proteinase inhibitor
Location	chr10: 6021716 .. 6022165 (+)
RNA-Seq Expression	PI0019573
Synteny	PI0019573

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAGAGAAAAAGTGATATTGAACATGGCTGAAGAAGATGTTATTATTCCTTCAGGTTAGTAATAATACTCCTTCCTAGACTTTTCCAAATAATTTCTTTTCTTCCCTTCAATTTCTCTTTTCAAAACTTATGAATAGTTAGTATTTACTTTTTGCGTAATTCAATTTGCTCAGGAAAATCTTCGTGGCCGGAGCTCGTGTTCGTCGAATCTTCAGTTGCAACACGTCTTATAGAGAGAGATAATCCCGATGTGAAGAAAATTGTTGTACTACTAGCTGGGTCTCCAGTTACTAGAGATTTCAAACTAGATCGAGTTCGAATTTTTGTTAATATAGAGAATAAGGTGGTTGAAGTTCCTAGAATTGGTTGAAATAATTTGAATATTTGTATCTGGAACAATTAAGATGATAAATAAGGTGGAATCTACATTTCTACCCTTTTAAATGACT

mRNA sequence

AATAGAGAAAAAGTGATATTGAACATGGCTGAAGAAGATGTTATTATTCCTTCAGGAAAATCTTCGTGGCCGGAGCTCGTGTTCGTCGAATCTTCAGTTGCAACACGTCTTATAGAGAGAGATAATCCCGATGTGAAGAAAATTGTTGTACTACTAGCTGGGTCTCCAGTTACTAGAGATTTCAAACTAGATCGAGTTCGAATTTTTGTTAATATAGAGAATAAGGTGGTTGAAGTTCCTAGAATTGGTTGAAATAATTTGAATATTTGTATCTGGAACAATTAAGATGATAAATAAGGTGGAATCTACATTTCTACCCTTTTAAATGACT

Coding sequence (CDS)

ATGGCTGAAGAAGATGTTATTATTCCTTCAGGAAAATCTTCGTGGCCGGAGCTCGTGTTCGTCGAATCTTCAGTTGCAACACGTCTTATAGAGAGAGATAATCCCGATGTGAAGAAAATTGTTGTACTACTAGCTGGGTCTCCAGTTACTAGAGATTTCAAACTAGATCGAGTTCGAATTTTTGTTAATATAGAGAATAAGGTGGTTGAAGTTCCTAGAATTGGTTGA

Protein sequence

MAEEDVIIPSGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVEVPRIG

Homology

[Close]

BLAST of PI0019573 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-13
Identity = 34/64 (53.12%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 11  GKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVE 70
           GK  WPEL+ V +  A  +IE++NP +  I +LL GSPVT+DF+ DRVR+FVNI   VV+
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 71  VPRI 75
           +PR+
Sbjct: 107 IPRV 110

[Close]

BLAST of PI0019573 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.1e-12
Identity = 39/65 (60.00%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 11 GKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVE 70
          GKSSWP LV V  SVA  +IER NP+V K V+L  G+PVT+DF+ +RVRI+VN    VV 
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNV-KAVILEEGTPVTKDFRCNRVRIWVNKRGLVVS 64

Query: 71 VPRIG 76
           PRIG
Sbjct: 65 PPRIG 68

[Close]

BLAST of PI0019573 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.6e-11
Identity = 30/62 (48.39%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 11  GKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVE 70
           GK  WPEL+ V + +A  +IE++NP +  I +LL+GSP+T D+  DRVR+F NI   VV+
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 71  VP 73
           +P
Sbjct: 107 MP 108

[Close]

BLAST of PI0019573 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 6.0e-11
Identity = 28/64 (43.75%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 12 KSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVEV 71
          K +WPEL+ V +  A  +I+++N  +  +  +L GSPVT+DF+ +RVR+FVN+ + VV++
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQI 90

Query: 72 PRIG 76
          PR+G
Sbjct: 91 PRVG 94

[Close]

BLAST of PI0019573 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 29/65 (44.62%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 10  SGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVV 69
           +GK  WPEL+ V + +A  +IE++N  +  + +LL GSPVT D++ +RVR+F NI   VV
Sbjct: 42  NGKQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVV 101

Query: 70  EVPRI 75
           ++PR+
Sbjct: 102 QIPRV 106

[Close]

BLAST of PI0019573 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.4e-18
Identity = 54/76 (71.05%), Postives = 62/76 (81.58%), Query Frame = 0


Query: 1  MAEEDVIIP-SGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVR 60
          MAEE ++ P SGKSSWPELVFV+SSVA   IERD PDVK  VVLLAGSPVT D + +RVR
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKP-VVLLAGSPVTEDLRPNRVR 60

Query: 61 IFVNIENKVVEVPRIG 76
          IFVN+ ++VVEVPR G
Sbjct: 61 IFVNMNDRVVEVPRTG 75

[Close]

BLAST of PI0019573 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.0e-14
Identity = 37/69 (53.62%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 7  IIPSGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIEN 66
          I P GK+ WPELV ++++ A  +I +DNP V+K++VLLAG+  T+D + DRV +FVNIE 
Sbjct: 4  ICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVNIEE 63

Query: 67 KVVEVPRIG 76
          KVVE+P++G
Sbjct: 64 KVVEIPKVG 72

[Close]

BLAST of PI0019573 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 6.7e-13
Identity = 38/69 (55.07%), Postives = 53/69 (76.81%), Query Frame = 0


Query: 7  IIPSGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIEN 66
          I P GK+ WPELV V+++ A  +I++DNP+V+ +VVLLAGS  T D + DRV +FVNI +
Sbjct: 4  ICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHD 63

Query: 67 KVVEVPRIG 76
           VVEVP++G
Sbjct: 64 LVVEVPKVG 72

[Close]

BLAST of PI0019573 vs. ExPASy TrEMBL
Match: A0A3Q7I868 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247557 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.7e-11
Identity = 34/64 (53.12%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 11  GKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVE 70
           GK  WPEL+ V +  A  +IE++NP +  I +LL GSPVT+DF+ DRVR+FVNI   VV+
Sbjct: 47  GKQFWPELIGVPAQYAKGIIEKENPSIANIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 71  VPRI 75
           +PR+
Sbjct: 107 IPRV 110

[Close]

BLAST of PI0019573 vs. ExPASy TrEMBL
Match: A0A067E312 (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033055mg PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.7e-11
Identity = 38/64 (59.38%), Postives = 49/64 (76.56%), Query Frame = 0


Query: 12  KSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVEV 71
           KSSWPELV     VA  +IE++NP V  I VLL G+PVTRD+++DRVR++VN + KV+ V
Sbjct: 66  KSSWPELVGKNGEVAAAIIEKENPCVHAI-VLLEGTPVTRDYRIDRVRVWVNKKGKVIRV 125

Query: 72  PRIG 76
           PRIG
Sbjct: 126 PRIG 128

[Close]

BLAST of PI0019573 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 101.7 bits (252), Expect = 2.9e-18
Identity = 54/76 (71.05%), Postives = 62/76 (81.58%), Query Frame = 0


Query: 1  MAEEDVIIP-SGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVR 60
          MAEE ++ P SGKSSWPELVFV+SSVA   IERD PDVK  VVLLAGSPVT D + +RVR
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKP-VVLLAGSPVTEDLRPNRVR 60

Query: 61 IFVNIENKVVEVPRIG 76
          IFVN+ ++VVEVPR G
Sbjct: 61 IFVNMNDRVVEVPRTG 75

[Close]

BLAST of PI0019573 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 86.3 bits (212), Expect = 1.2e-13
Identity = 37/69 (53.62%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 7  IIPSGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIEN 66
          I P GK+ WPELV ++++ A  +I +DNP V+K++VLLAG+  T+D + DRV +FVNIE 
Sbjct: 4  ICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVNIEE 63

Query: 67 KVVEVPRIG 76
          KVVE+P++G
Sbjct: 64 KVVEIPKVG 72

[Close]

BLAST of PI0019573 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 82.8 bits (203), Expect = 1.4e-12
Identity = 38/69 (55.07%), Postives = 53/69 (76.81%), Query Frame = 0


Query: 7  IIPSGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIEN 66
          I P GK+ WPELV V+++ A  +I++DNP+V+ +VVLLAGS  T D + DRV +FVNI +
Sbjct: 4  ICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHD 63

Query: 67 KVVEVPRIG 76
           VVEVP++G
Sbjct: 64 LVVEVPKVG 72

[Close]

BLAST of PI0019573 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 78.2 bits (191), Expect = 3.4e-11
Identity = 34/67 (50.75%), Postives = 50/67 (74.63%), Query Frame = 0


Query: 9  PSGKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKV 68
          P GK+ WPELV ++S++A  +I +DN +V+ +  +LAGS VT D + DRVRIFVNI+  +
Sbjct: 6  PPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKGII 65

Query: 69 VEVPRIG 76
          V++P IG
Sbjct: 66 VQIPTIG 72

[Close]

BLAST of PI0019573 vs. NCBI nr
Match: NP_001315434.1 (proteinase inhibitor I precursor [Solanum lycopersicum])

HSP 1 Score: 77.0 bits (188), Expect = 7.6e-11
Identity = 34/64 (53.12%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 11  GKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVE 70
           GK  WPEL+ V +  A  +IE++NP +  I +LL GSPVT+DF+ DRVR+FVNI   VV+
Sbjct: 47  GKQFWPELIGVPAQYAKGIIEKENPSIANIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 71  VPRI 75
           +PR+
Sbjct: 107 IPRV 110

[Close]

BLAST of PI0019573 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 62.0 bits (149), Expect = 2.4e-10
Identity = 32/64 (50.00%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 12 KSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVEV 71
          K+SWPEL       A  +IER+NP V    V+L GSPVT DF+ DRVR+FV+    VV+ 
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTV-NAAVILDGSPVTADFRCDRVRVFVDGNRIVVKT 67

Query: 72 PRIG 76
          P+ G
Sbjct: 68 PKSG 70

[Close]

BLAST of PI0019573 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.9e-07
Identity = 29/65 (44.62%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 11 GKSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVE 70
          GKSSWPEL+  +   A  +IER+NP + K V++L G+ V   F   RV ++VN    VV+
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKM-KAVIILDGTVVPEIFICSRVYVWVNDCGIVVQ 95

Query: 71 VPRIG 76
          +P IG
Sbjct: 96 IPIIG 99

[Close]

BLAST of PI0019573 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 42.0 bits (97), Expect = 2.5e-04
Identity = 24/64 (37.50%), Postives = 34/64 (53.12%), Query Frame = 0


Query: 12 KSSWPELVFVESSVATRLIERDNPDVKKIVVLLAGSPVTRDFKLDRVRIFVNIENKVVEV 71
          K  WPELV     +A   IER+NP+V  I VL  G     +F  +RV +++    +V + 
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAI-VLRYGEKRIENFCCNRVFVYLGSNGQVADA 82

Query: 72 PRIG 76
          P IG
Sbjct: 83 PMIG 85

The following BLAST results are available for this feature:

BLAST of PI0019573 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	1.7e-13	53.13	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P19873	1.1e-12	60.00	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P05118	4.6e-11	48.39	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
Q02214	6.0e-11	43.75	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P08454	1.3e-10	44.62	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]

BLAST of PI0019573 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPI0	1.4e-18	71.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A0M3SAG8	6.0e-14	53.62	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KME7	6.7e-13	55.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A3Q7I868	3.7e-11	53.13	Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247557 PE=3 SV=1	[more]
A0A067E312	3.7e-11	59.38	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033055mg PE=3 SV=1	[more]

BLAST of PI0019573 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654473.1	2.9e-18	71.05	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
ALD47589.1	1.2e-13	53.62	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50840.1	1.4e-12	55.07	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
KAG6571494.1	3.4e-11	50.75	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
NP_001315434.1	7.6e-11	53.13	proteinase inhibitor I precursor [Solanum lycopersicum]	[more]

BLAST of PI0019573 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	2.4e-10	50.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.9e-07	44.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	2.5e-04	37.50	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 52..62 score: 52.96 coord: 40..51 score: 47.83 coord: 14..27 score: 43.48
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 12..75 e-value: 8.7E-24 score: 84.0
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 7..75
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 8..75 e-value: 2.4E-23 score: 84.1
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 7..75
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 9..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019573.1	PI0019573.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity