PI0019477 (gene) Melon (PI 482460) v1

BLAST of PI0019477 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.4e-64
Identity = 142/338 (42.01%), Postives = 199/338 (58.88%), Query Frame = 0

Query: 8   FLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQGSIGFFIG 67
           F++   LV  SL + F I   +P G  + +L  P F +F  +P    +LHL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEI-----PQDTNRSDPNPILPLS 127
           WLA+FKLLLFA G+GPL S     SLP FLA+  LPI+I     P  T+  + +   PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 FFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELLPY 187
           + IK   ++L   A  +   L   V L ++   IYF LEI++   AA V+A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAVSG 247
           F++PYL+ SLQDFWG+RWNLM + ILR  VY+P  +L   ++G   + ++A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWYVM 307
           +MHELI+FYMGRL P W++  FFL++G   + E+A+K  + GR R P  +S   T  +VM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 RTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQL 341
            T+ WLF P+F R     +  +E+ A+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

BLAST of PI0019477 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.9e-55
Identity = 134/347 (38.62%), Postives = 194/347 (55.91%), Query Frame = 0

Query: 4   EIRIFLQTLFLVSSSLSFCFSIRNSIPN-GFLKFLLILPFFFLFLYIPLQFQTLHLQGSI 63
           E++ F +      ++  +C  +    P+ G L+ LL+LP   LF+++PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILPLS 123
             ++ WLA+FKLLLFAF  GPL +P  S SL  F++   LPI+   D +         LS
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSNP--SLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 124 F-------FIKFGLLILTFVAIY-FKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKAT 183
           F       F+   +     + IY F+  L   V  V++CF  Y   EI +  +A   +AT
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARAT 185

Query: 184 LGVELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAV 243
           LG++L P F+EPYL+ SLQDFWG+RWNLM S IL L  Y P R++    +  +     A+
Sbjct: 186 LGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAM 245

Query: 244 TATFAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVST 303
              F VSGLMHE+ +FY+ R  PSWEVT FF+LHGV  + EM +K AV G++R+   VS 
Sbjct: 246 LVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSG 305

Query: 304 AFTWWYVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLI 342
           A T  +VM T  WLF PQ  R   D++  +E+  M  + +   + L+
Sbjct: 306 ALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 350

BLAST of PI0019477 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 8.6e-52
Identity = 133/334 (39.82%), Postives = 189/334 (56.59%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E+R  ++       S+S+C+ I + I  G L+ L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQD--TNRSDPNPI 120
             GFF  WLA+FKL LFAF + PL SP   S+L RF      PI+I ++  +NR    P+
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SP-LPSNLTRFFCFACFPIKINKNPSSNRIHNKPM 120

Query: 121 LPLSFFIKFGLLILTFVA-IYFKNY---LHPDVYLVVFCFLIYFLLEILIGGAAAFVKAT 180
               + + F LLI +F+  +Y  NY   L    +L +F   +Y   E+++    A +   
Sbjct: 121 --SKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAV 240
           LG E+ P F++PYL+ SLQ+FW +RWNLM   +LR AV+ P ++    ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 TATFAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKS---AVRGRLRVPGI 300
            ATF VSGLMHELIYFY+ R +P+WEVTCFFLLHGV    E+AMK        R  V G+
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRMRWLPTPRRAVSGL 300

Query: 301 VSTAFTWWYVMRTSSWLFFPQFFRLKPDVRMFEE 326
             T F    ++ T+ WLF+PQ  R     R+  E
Sbjct: 301 AITVF----LLVTAGWLFYPQMLRNDVHKRVISE 326

BLAST of PI0019477 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.3e-51
Identity = 132/349 (37.82%), Postives = 189/349 (54.15%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E+   ++       S+S+C+     I +G  + L +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILP 120
           S  FF+ WLA+FKL+LF+F +GPL      S+L RF+     PI++ Q  N    N +  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF--PVPSNLSRFVCFTCFPIKLQQ--NPKPQNQMPK 120

Query: 121 LSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELL 180
             F +K     +      +K ++ P V LV++   IY   EIL+      +  +L  +L 
Sbjct: 121 WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLE 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAV 240
           P+F+EPYLS SLQDFWG+RWNLM   ILR AVY P R++    +    A  + V A+F V
Sbjct: 181 PHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLV 240

Query: 241 SGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMK-SAVRGRLRVPGIVSTAFTWW 300
           SG++HELI+FY  R +P+ EVT FF+LHGV  +AE A K + +  R +V  +VS   T  
Sbjct: 241 SGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVG 300

Query: 301 YVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKFFY 349
           +V+ T  WLFFP   R     R+ +E      FVK+         KFFY
Sbjct: 301 FVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH---------KFFY 336

BLAST of PI0019477 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.7e-50
Identity = 130/314 (41.40%), Postives = 179/314 (57.01%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E++ F++       S+S+C+ +   I  G  + L +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILP 120
            + FF+ WLA+FKL+LF+F +GPL SP    +L RF+ I   PI+  Q+ N    N  +P
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SP-LPRTLSRFICITCFPIKPQQNPNIQ--NYKIP 120

Query: 121 LSFF-IKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVEL 180
           +  F IK  + ++      +K YL P + LV     I+  LEI+     A V  TLG +L
Sbjct: 121 IWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFA 240
            P  +EPYL+ SLQDFWG+RWNLM   ILR AVY P R++    +    A  + V A F 
Sbjct: 181 EPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFL 240

Query: 241 VSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSA--VRGRLRVPGIVSTAFT 300
           VSG +HE+++FY+ R  P+ EVT FFLLHGV   AE+A+K +  VR   RV   VS   T
Sbjct: 241 VSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLT 300

Query: 301 WWYVMRTSSWLFFP 312
             +V+ TS W FFP
Sbjct: 301 VGFVVVTSGWFFFP 310

BLAST of PI0019477 vs. ExPASy TrEMBL
Match: A0A0A0LES4 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684670 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.6e-173
Identity = 314/348 (90.23%), Postives = 331/348 (95.11%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           MAAEIRIFLQTL LVSSSLSFCFSIRNSIPNGFL+FLLILPFF LFLYIPLQFQT+HLQG
Sbjct: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILP 120
           SIGFFIGWLASFKLLLFAFGKGPLCS AASSSL RFLAIGSLPIEIPQDTNRS PNPILP
Sbjct: 61  SIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120

Query: 121 LSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELL 180
            ++FIK  LLILT VAIYFKNYLHP +YL+VFCF+IYFLLEILIGGAAAFVKATLGVELL
Sbjct: 121 PTYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAV 240
           PYFDEPYLSVSLQDFWGKRWNL+TSRILRLAVYDPCRKLTVGI+GKK +AMVA+TATFAV
Sbjct: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTATFAV 240

Query: 241 SGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWY 300
           SGLMHELIYFYMGRLAP+WEVTCFFLLHGVSLSAEMAMKSAVRGRL+VP I+ST FTWW+
Sbjct: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300

Query: 301 VMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKFFY 349
           VM TSSWLFFPQFFRLKPDVRM EEH A+GAFVKNVTVQLITPFKF +
Sbjct: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 348

BLAST of PI0019477 vs. ExPASy TrEMBL
Match: A0A5D3BDP7 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold939G00060 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.6e-171
Identity = 306/345 (88.70%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 2   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQGS 61
           AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGF KFL +LPFFFLFLY+PLQFQTLHLQG 
Sbjct: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62

Query: 62  IGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILPL 121
            GFFIGWLASFKLLLFAFGKGPL SPAASSSL RFLAIGSLPIEIPQDTN SDPNPILP 
Sbjct: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPH 122

Query: 122 SFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELLP 181
           ++FIK GLLILTFVA+YF+ YLHP++YL+ FCFLIYFLLEILIGGAAAFVKATLGVELLP
Sbjct: 123 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 182

Query: 182 YFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAVS 241
           YFDEPYLS SLQDFWGKRWNLM SRILRLAVYDPCRKLT+GI+GKKP+A+VA+TATFAVS
Sbjct: 183 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 242

Query: 242 GLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWYV 301
           GLMHELIYFYMGR+AP+WEVTCFFLLHGVSLSAEMAMKSAV GR RVP IVST FTWW+V
Sbjct: 243 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 302

Query: 302 MRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF 347
           M TSSWLFFPQFFRLKP+VRMFEEH A+ AFVKNVTVQLITPFKF
Sbjct: 303 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 347

BLAST of PI0019477 vs. ExPASy TrEMBL
Match: A0A1S3CFI3 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500342 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.6e-171
Identity = 306/345 (88.70%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 2   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQGS 61
           AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGF KFL +LPFFFLFLY+PLQFQTLHLQG 
Sbjct: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62

Query: 62  IGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILPL 121
            GFFIGWLASFKLLLFAFGKGPL SPAASSSL RFLAIGSLPIEIPQDTN SDPNPILP 
Sbjct: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPH 122

Query: 122 SFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELLP 181
           ++FIK GLLILTFVA+YF+ YLHP++YL+ FCFLIYFLLEILIGGAAAFVKATLGVELLP
Sbjct: 123 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 182

Query: 182 YFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAVS 241
           YFDEPYLS SLQDFWGKRWNLM SRILRLAVYDPCRKLT+GI+GKKP+A+VA+TATFAVS
Sbjct: 183 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 242

Query: 242 GLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWYV 301
           GLMHELIYFYMGR+AP+WEVTCFFLLHGVSLSAEMAMKSAV GR RVP IVST FTWW+V
Sbjct: 243 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 302

Query: 302 MRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF 347
           M TSSWLFFPQFFRLKP+VRMFEEH A+ AFVKNVTVQLITPFKF
Sbjct: 303 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 347

BLAST of PI0019477 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.6e-141
Identity = 253/289 (87.54%), Postives = 270/289 (93.43%), Query Frame = 0

Query: 58  LQGSIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNP 117
           L G  GFFIGWLASFKLLLFAFGKGPL SPAASSSL RFLAIGSLPIEIPQDTN SDPNP
Sbjct: 348 LFGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP 407

Query: 118 ILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGV 177
           ILP ++FIK GLLILTFVA+YF+ YLHP++YL+ FCFLIYFLLEILIGGAAAFVKATLGV
Sbjct: 408 ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV 467

Query: 178 ELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTAT 237
           ELLPYFDEPYLS SLQDFWGKRWNLM SRILRLAVYDPCRKLT+GI+GKKP+A+VA+TAT
Sbjct: 468 ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT 527

Query: 238 FAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFT 297
           FAVSGLMHELIYFYMGR+AP+WEVTCFFLLHGVSLSAEMAMKSAV GR RVP IVST FT
Sbjct: 528 FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT 587

Query: 298 WWYVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF 347
           WW+VM TSSWLFFPQFFRLKP+VRMFEEH A+ AFVKNVTVQLITPFKF
Sbjct: 588 WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 636

BLAST of PI0019477 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 8.4e-50
Identity = 128/297 (43.10%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  EI  FL+        LS+C+ +   IP+G  +FL ILP   LFLY+PL   +LHL G
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQD----------- 120
              FFI WLA+FKLLLF+ G GPL +P    SL  F AI S P+++ Q+           
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGI--SLGTFCAIASFPVKLKQNPLQKASEFAIN 120

Query: 121 ---TNRSDPN--PILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILI 180
               ++SD N  P  PLS+ +K  L+     A  + + L   ++ +  CF IYFLLE+ +
Sbjct: 121 GELPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 GGAAAFVKATLGVELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGII 240
             AA+ V     VELLP F EPY S SLQDFWG+RWNL+ + ILR AV+ P       + 
Sbjct: 181 AAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASKLF 240

Query: 241 GKKPAAMVAVTATFAVSGLMHELIYFYMGRL--APSWEVTCFFLLHGVSLSAEMAMK 280
           G +   +  VTATF VS +MHEL++FYM R    P+WEV+ FF+ HG  L  EM +K
Sbjct: 241 GSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVK 295

HSP 2 Score: 491.5 bits (1264), Expect = 2.9e-135
Identity = 250/346 (72.25%), Postives = 287/346 (82.95%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           MAAEI  F+QTLFL+SSSL  CFSIRNSIPNGFLKF+ +LPFFFLFLYIPLQFQT+HL+G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQ---DTNRSDPNP 120
           ++GFFIGWL SFKLLLFAFGKGPLCS A SSSL RFLA+ SLPI+IP+     NRSDP+P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 ILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGV 180
           +LPLSF  K G LILTF AIYFKN  +P+V L+ FC LIY LLEILIGGA A  +A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTAT 240
           ELLP+FDEPYLS SLQDFWG+RWNL++SRILR AV+DPCRKL   ++GKKPA ++AV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFT 300
           FAVSG+MHELIYFYMGRLAP+WEV+ FFL+HGV L+AEMA+KSAV  R R P +VS   T
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 WWYVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITP 344
             +VM TS WLFFPQ  R K DVRM EE+ A+GAFVKNVT +LI P
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGP 346

BLAST of PI0019477 vs. NCBI nr
Match: XP_004149741.2 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58561.1 hypothetical protein Csa_001738 [Cucumis sativus])

HSP 1 Score: 618.6 bits (1594), Expect = 3.3e-173
Identity = 314/348 (90.23%), Postives = 331/348 (95.11%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           MAAEIRIFLQTL LVSSSLSFCFSIRNSIPNGFL+FLLILPFF LFLYIPLQFQT+HLQG
Sbjct: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILP 120
           SIGFFIGWLASFKLLLFAFGKGPLCS AASSSL RFLAIGSLPIEIPQDTNRS PNPILP
Sbjct: 61  SIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILP 120

Query: 121 LSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELL 180
            ++FIK  LLILT VAIYFKNYLHP +YL+VFCF+IYFLLEILIGGAAAFVKATLGVELL
Sbjct: 121 PTYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELL 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAV 240
           PYFDEPYLSVSLQDFWGKRWNL+TSRILRLAVYDPCRKLTVGI+GKK +AMVA+TATFAV
Sbjct: 181 PYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTATFAV 240

Query: 241 SGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWY 300
           SGLMHELIYFYMGRLAP+WEVTCFFLLHGVSLSAEMAMKSAVRGRL+VP I+ST FTWW+
Sbjct: 241 SGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWF 300

Query: 301 VMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKFFY 349
           VM TSSWLFFPQFFRLKPDVRM EEH A+GAFVKNVTVQLITPFKF +
Sbjct: 301 VMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKFSF 348

BLAST of PI0019477 vs. NCBI nr
Match: XP_008461835.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >TYJ97433.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 609.4 bits (1570), Expect = 2.0e-170
Identity = 306/345 (88.70%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 2   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQGS 61
           AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGF KFL +LPFFFLFLY+PLQFQTLHLQG 
Sbjct: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62

Query: 62  IGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILPL 121
            GFFIGWLASFKLLLFAFGKGPL SPAASSSL RFLAIGSLPIEIPQDTN SDPNPILP 
Sbjct: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPH 122

Query: 122 SFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELLP 181
           ++FIK GLLILTFVA+YF+ YLHP++YL+ FCFLIYFLLEILIGGAAAFVKATLGVELLP
Sbjct: 123 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 182

Query: 182 YFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAVS 241
           YFDEPYLS SLQDFWGKRWNLM SRILRLAVYDPCRKLT+GI+GKKP+A+VA+TATFAVS
Sbjct: 183 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 242

Query: 242 GLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWYV 301
           GLMHELIYFYMGR+AP+WEVTCFFLLHGVSLSAEMAMKSAV GR RVP IVST FTWW+V
Sbjct: 243 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 302

Query: 302 MRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF 347
           M TSSWLFFPQFFRLKP+VRMFEEH A+ AFVKNVTVQLITPFKF
Sbjct: 303 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 347

BLAST of PI0019477 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 542.3 bits (1396), Expect = 3.0e-150
Identity = 280/353 (79.32%), Postives = 307/353 (86.97%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           MAAEIRIFLQTLFLVSSSL FCF+IRNSIPNGFLKFLLILPFFFLFLYIPLQFQ++H QG
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQD---TNRSDPNP 120
           SIGFFIGWL SFKLLLFAFGKGPLCS AASSSL  FLA+GSLPIEIP      N S  NP
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 ILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGV 180
           +LPLSF  K GLLILT VAIYFKN  HP+  L+ FCF+IY LLEIL+GGAAA  KATLGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 ELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTAT 240
           ELLPYFDEPYLS SLQDFWG+RWNLMTSRILRL VYDPCRK+T+GI+G+KPAAM+A+TAT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFT 300
           FAVSGLMHELIYFYMGRLAP+WEVTCFFLLHGV L+AEMA+KSAV GR R+PG+VS A T
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 WWYVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF-FYF 350
             +VM TS WLFFPQF +LK DVRMFEE+ A+GAFVK+VT QLITP    F+F
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLNISFHF 353

BLAST of PI0019477 vs. NCBI nr
Match: KAA0048417.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 511.1 bits (1315), Expect = 7.3e-141
Identity = 253/289 (87.54%), Postives = 270/289 (93.43%), Query Frame = 0

Query: 58  LQGSIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNP 117
           L G  GFFIGWLASFKLLLFAFGKGPL SPAASSSL RFLAIGSLPIEIPQDTN SDPNP
Sbjct: 348 LFGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP 407

Query: 118 ILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGV 177
           ILP ++FIK GLLILTFVA+YF+ YLHP++YL+ FCFLIYFLLEILIGGAAAFVKATLGV
Sbjct: 408 ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV 467

Query: 178 ELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTAT 237
           ELLPYFDEPYLS SLQDFWGKRWNLM SRILRLAVYDPCRKLT+GI+GKKP+A+VA+TAT
Sbjct: 468 ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT 527

Query: 238 FAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFT 297
           FAVSGLMHELIYFYMGR+AP+WEVTCFFLLHGVSLSAEMAMKSAV GR RVP IVST FT
Sbjct: 528 FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT 587

Query: 298 WWYVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF 347
           WW+VM TSSWLFFPQFFRLKP+VRMFEEH A+ AFVKNVTVQLITPFKF
Sbjct: 588 WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 636

BLAST of PI0019477 vs. NCBI nr
Match: KAA0048417.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 207.6 bits (527), Expect = 1.7e-49
Identity = 128/297 (43.10%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  EI  FL+        LS+C+ +   IP+G  +FL ILP   LFLY+PL   +LHL G
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQD----------- 120
              FFI WLA+FKLLLF+ G GPL +P    SL  F AI S P+++ Q+           
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGI--SLGTFCAIASFPVKLKQNPLQKASEFAIN 120

Query: 121 ---TNRSDPN--PILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILI 180
               ++SD N  P  PLS+ +K  L+     A  + + L   ++ +  CF IYFLLE+ +
Sbjct: 121 GELPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 GGAAAFVKATLGVELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGII 240
             AA+ V     VELLP F EPY S SLQDFWG+RWNL+ + ILR AV+ P       + 
Sbjct: 181 AAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASKLF 240

Query: 241 GKKPAAMVAVTATFAVSGLMHELIYFYMGRL--APSWEVTCFFLLHGVSLSAEMAMK 280
           G +   +  VTATF VS +MHEL++FYM R    P+WEV+ FF+ HG  L  EM +K
Sbjct: 241 GSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVK 295

HSP 2 Score: 494.6 bits (1272), Expect = 7.1e-136
Identity = 256/349 (73.35%), Postives = 282/349 (80.80%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           MAAEI   LQTLFLVSSSL FCF+IRNSIPNGFLKFLLILPFFFLFLYIPLQFQT+H QG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTIHFQG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIP---QDTNRSDPNP 120
            IGFFIGWL SFKLLLFAFGKGPL S A SSSLPRFLA+GSLPIEIP      N S+P  
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLSSAATSSSLPRFLAVGSLPIEIPDHQSPPNHSNPKL 120

Query: 121 ILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGV 180
           +LP S+  K   LILT VAI FKN+ HP+  L+ +C LIYF LE LIG  AA  KA LGV
Sbjct: 121 VLPFSYSAKLAFLILTMVAINFKNHFHPNAALLFYCLLIYFFLEFLIGSTAAVAKAVLGV 180

Query: 181 ELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTAT 240
           ELLPYF+EPYLS SLQDFWG+RWNLMTSRILRL VYDPCRKLT+G+IG+KPA M+AV AT
Sbjct: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTIGVIGRKPATMLAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFT 300
           F VSGLMHELIYFY+GRLAP+WEVTCFFL+HGV L  EMA+K A  GR R P +VS   T
Sbjct: 241 FVVSGLMHELIYFYIGRLAPTWEVTCFFLVHGVCLVTEMAVKLAAGGRFRPPQVVSIPLT 300

Query: 301 WWYVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKF 347
             +VM T  WLFFPQ  R K DVRMFEE+ A+GAFVK++TV LITPFKF
Sbjct: 301 IGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKSITVDLITPFKF 349

BLAST of PI0019477 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 246.9 bits (629), Expect = 2.4e-65
Identity = 142/338 (42.01%), Postives = 199/338 (58.88%), Query Frame = 0

Query: 8   FLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQGSIGFFIG 67
           F++   LV  SL + F I   +P G  + +L  P F +F  +P    +LHL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEI-----PQDTNRSDPNPILPLS 127
           WLA+FKLLLFA G+GPL S     SLP FLA+  LPI+I     P  T+  + +   PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 FFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELLPY 187
           + IK   ++L   A  +   L   V L ++   IYF LEI++   AA V+A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAVSG 247
           F++PYL+ SLQDFWG+RWNLM + ILR  VY+P  +L   ++G   + ++A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVRGRLRVPGIVSTAFTWWYVM 307
           +MHELI+FYMGRL P W++  FFL++G   + E+A+K  + GR R P  +S   T  +VM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 RTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQL 341
            T+ WLF P+F R     +  +E+ A+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

BLAST of PI0019477 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 205.7 bits (522), Expect = 6.1e-53
Identity = 133/334 (39.82%), Postives = 189/334 (56.59%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E+R  ++       S+S+C+ I + I  G L+ L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQD--TNRSDPNPI 120
             GFF  WLA+FKL LFAF + PL SP   S+L RF      PI+I ++  +NR    P+
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SP-LPSNLTRFFCFACFPIKINKNPSSNRIHNKPM 120

Query: 121 LPLSFFIKFGLLILTFVA-IYFKNY---LHPDVYLVVFCFLIYFLLEILIGGAAAFVKAT 180
               + + F LLI +F+  +Y  NY   L    +L +F   +Y   E+++    A +   
Sbjct: 121 --SKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAV 240
           LG E+ P F++PYL+ SLQ+FW +RWNLM   +LR AV+ P ++    ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 TATFAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKS---AVRGRLRVPGI 300
            ATF VSGLMHELIYFY+ R +P+WEVTCFFLLHGV    E+AMK        R  V G+
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRMRWLPTPRRAVSGL 300

Query: 301 VSTAFTWWYVMRTSSWLFFPQFFRLKPDVRMFEE 326
             T F    ++ T+ WLF+PQ  R     R+  E
Sbjct: 301 AITVF----LLVTAGWLFYPQMLRNDVHKRVISE 326

BLAST of PI0019477 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.3e-52
Identity = 132/349 (37.82%), Postives = 189/349 (54.15%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E+   ++       S+S+C+     I +G  + L +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILP 120
           S  FF+ WLA+FKL+LF+F +GPL      S+L RF+     PI++ Q  N    N +  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF--PVPSNLSRFVCFTCFPIKLQQ--NPKPQNQMPK 120

Query: 121 LSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVELL 180
             F +K     +      +K ++ P V LV++   IY   EIL+      +  +L  +L 
Sbjct: 121 WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLE 180

Query: 181 PYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFAV 240
           P+F+EPYLS SLQDFWG+RWNLM   ILR AVY P R++    +    A  + V A+F V
Sbjct: 181 PHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLV 240

Query: 241 SGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMK-SAVRGRLRVPGIVSTAFTWW 300
           SG++HELI+FY  R +P+ EVT FF+LHGV  +AE A K + +  R +V  +VS   T  
Sbjct: 241 SGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVG 300

Query: 301 YVMRTSSWLFFPQFFRLKPDVRMFEEHEAMGAFVKNVTVQLITPFKFFY 349
           +V+ T  WLFFP   R     R+ +E      FVK+         KFFY
Sbjct: 301 FVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH---------KFFY 336

BLAST of PI0019477 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.9 bits (507), Expect = 3.4e-51
Identity = 130/314 (41.40%), Postives = 179/314 (57.01%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E++ F++       S+S+C+ +   I  G  + L +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEIPQDTNRSDPNPILP 120
            + FF+ WLA+FKL+LF+F +GPL SP    +L RF+ I   PI+  Q+ N    N  +P
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SP-LPRTLSRFICITCFPIKPQQNPNIQ--NYKIP 120

Query: 121 LSFF-IKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATLGVEL 180
           +  F IK  + ++      +K YL P + LV     I+  LEI+     A V  TLG +L
Sbjct: 121 IWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDL 180

Query: 181 LPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVTATFA 240
            P  +EPYL+ SLQDFWG+RWNLM   ILR AVY P R++    +    A  + V A F 
Sbjct: 181 EPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFL 240

Query: 241 VSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSA--VRGRLRVPGIVSTAFT 300
           VSG +HE+++FY+ R  P+ EVT FFLLHGV   AE+A+K +  VR   RV   VS   T
Sbjct: 241 VSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLT 300

Query: 301 WWYVMRTSSWLFFP 312
             +V+ TS W FFP
Sbjct: 301 VGFVVVTSGWFFFP 310

BLAST of PI0019477 vs. TAIR 10
Match: AT5G55330.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 129/322 (40.06%), Postives = 180/322 (55.90%), Query Frame = 0

Query: 1   MAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTLHLQG 60
           M  E+++F+Q       S+++C+ +   I    L+ L +LP   LFL IP+ F T+H   
Sbjct: 1   MEEELKLFIQVWVSAIISVTYCYYLTPKIKTSLLRLLSVLPVCVLFLIIPIFFSTVHSSF 60

Query: 61  SIGFFIGWLASFKLLLFAFGKGPLCSPAASSSLPRFLAIGSLPIEI-----PQDTNRSDP 120
           +I FF+  LA  KL+LFA  KGPL       +LP F+     PI++     P++TN   P
Sbjct: 61  TIAFFLSGLAVPKLILFALEKGPLF--PLPPNLPHFVCFACFPIKLQKKPNPENTNHF-P 120

Query: 121 NPILPLSFFIKFGLLILTFVAIYFKNYLHPDVYLVVFCFLIYFLLEILIGGAAAFVKATL 180
             +  L  FI FG L+L   A ++K +L  +  L ++   IY  LEI +      V  TL
Sbjct: 121 KWVFALKVFI-FGALLLQ--AYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITL 180

Query: 181 GVELLPYFDEPYLSVSLQDFWGKRWNLMTSRILRLAVYDPCRKLTVGIIGKKPAAMVAVT 240
           G +L P F+EPYL+ SL DFWG RWNLM S+IL LAVY+P R+        +     A+ 
Sbjct: 181 GCDLEPQFNEPYLATSLHDFWGHRWNLMVSKILWLAVYNPIRQWRAK--SSEWDRFFAIF 240

Query: 241 ATFAVSGLMHELIYFYMGRLAPSWEVTCFFLLHGVSLSAEMAMKSAVR--GRLRVPGIVS 300
           ATF VSG+ HE++YFY+ R  P+WEVT FF+LHG  ++AE+A+K   +   R  V   VS
Sbjct: 241 ATFLVSGVAHEILYFYLTREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVS 300

Query: 301 TAFTWWYVMRTSSWLFFPQFFR 316
              T  +V  T  WLF PQ  R
Sbjct: 301 RLLTVGFVFVTGVWLFSPQPIR 314