PI0019452 (gene) Melon (PI 482460) v1

Overview
NamePI0019452
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMalic enzyme
Locationchr11: 9141 .. 23817 (+)
RNA-Seq ExpressionPI0019452
SyntenyPI0019452
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCCTCTCCGCAATTATTCCTCCATTATTCGTCAACAGCTTCAACTGCCCCGCTATAATTTGACCGCAACTTTTATGTTTCAACTGAACAAAGCCTCTGAAACCATTACAACCTTAACTAGCATTGCTTCGAATTACAGCGTCAATCCCGCGAACTTTGGGATCTTTTTTTTAATCTTTTTGGAAGATTGGTTGGACGCTTGAAGTAGTAGAGCACTGGATTTGCGGCACTGCTGCGTTGGGTTTACTGGTAGCCAGATTGGATTTGGTTTCTGCTTTCTTGATTTTTCCCTAGATCCAGCTTTATGTGGAACCTTGTGAAGTTTACTGCAGCGAGTTTTCGCCGATCTAGGCGGTTGTCGACTGCGATTCCTGGTCCTTGTATTGTCCATAAGCGCGGCGCTGATATTCTACACGATCCTTGGTTCAACAAGGTTTGTTGCTTTTGTTTTCTTTGGTCCTTTCATGTATGTTAGTTTCTCCGTTAGTTGAAATTGATCTGTACTTTTTCCTCTCAAAAGTAGAAATTTTAGTCTCTTACTCAAAAGTTGAAAAGACGATCCAAAAGTGTTTATATATATATGAAAAAAATTCTATAAAAAAAATATAGAAACAAAGGTGTAACTGTGAAGAGTACATTTGAGAACTGTTGTAATTTTCCAAGGGCATTGTTTTATTGCTATTAATTAATTATTTGTTCAGTAGAAGTAATGAAATTTAAGCATTGTTGTCTATATTCTGGTATAAGCGTGTGGACGAATGCAAGTGTTTTGTAAAAATGAAATATGAATATACAATGCCTTATAATTTGAAGATAGGACTGGACTTTTTGGTGTTTCCAGATTTGAAGGTGTCTCCATTTAGGGTGATAATTGGAACATATTTATGAACTCTGTACTTGCACTAGAATGTTTTAACACGGTAAATGAATCGAGCTCTTATTAGTTACTGTACTTTTAAAGCCTGTCTGTTAGTACAATTGCCATTTTGAGCATAACTTTAAAGCATGTTCACTAGTATCAAGAGGTTAGAGGTTCAGATCTTCTACCTTAGCACTCATTGAACTCTAAATAAAATAATAGTAATAATAATAATAAATAAAAAGTCCGTTCATAAATACCCTTTGGGACTTCTTTTTTAAACGGGTTTTGATTCTTCAACTGGGTAAGGTTTCTGGCACAAGGAGATCGACGTGTGATCATCACTTTTAAATTCTTTATCAGTATGCATTTTACTCTTTTTTTATTGTTAAATTATGAAAATTTGTAGTACCAAATCTGCTTCTTCTCTTTTTATATTTCTAAAAAGAAAATTTTTCTTTATCCCTTGGTTGACTGTCATGGTCATGTGCAAGTCTCTTTACATTTGTCTCTTTCTATTTTCAAAGAGCAGGATACTGGATTTCCTCTCACTGAACGAGATCGTCTAGGAATCCGTGGCCTTCTTCCTCCACGAGTAATATCATTTGAGCAGCAATATGCTCGTTTCAGTAAGTTTATGCTTGATCTTAAATTTCATTGCTTGTCTATCATTGTAATGATTTAGTATGGATCCCCTAAAATAGAAATACATTAGTGGATTTGCTATAATGAAAGATTTGCATTTTACCTTTATTTCACCGTTAGTCTCGTTACACATTGTTTGGTAGGATATTACTTGGATGCTATGCTAGCTGTTAAATGTAAATTGCTAGTAGTTTGGCTGTTTAACCTTTTATTTGGCTATTATTTTTCTAGTTCGTTGAATGCAACTTTGTTGGTCATTTGCAACACTTAATGGTAGGTTATTGCCCTTGTAATCACAAATTTTATTTAAAAGAAACATTTATTTTGATTAGTTGAAAACTTGTGACTCTATTTTATTGCTCCTGCCATAGTTACAAAAAGCCACTTCAAGTTAAGTTAATTGCAATTTGTTGAAGTAATATAATTACAAAAGGAGTTGAATTATTTGATATCCAATTGGAGATATTTTATGTTATTCACCTTTTGGTGTTGTGATTCCAAAGAGCTTATTTTATTTCATTAGCCAATGAAATTTGTTTCTATTCTAAGGAAGTTCTTGTAGTCACAAGTCTGGTACTGAATACAGATTTCTTTTAGTATTGGAGAGTTTCAATCCTTAGGAGCATTAGAGATAAGATCATTTCTTTGAATGTCTCTTGCATGTTTTCGTTCAATTTGCTAGTGGAATCATATCGGTCGCTGGAGAGGAATACTCATGGTCAACCAGATGGCATTGTTTCATTAGCGAAATGGAGGATTTTAAACAGATTGCATGATAGGAATGAGACACTGTACTATCGAGTAAGTTGCAAAATTATTGCTGTTTAGGTGCTTGTAAGTAAATATTTGTTTTGATCCAATTTTATTGTTTTTTCGCTTTAGGTCCTTATTGATAACATAAAAAGCTTTGCTCCAATAATATACACACCTACAGTGGGATTAGTTTGTCAAAACTATTCTGGGTTATTCCGACGTCCACGTGGAATGTATTTCAGTGCAAAGGACAAAGGAGAAATGATGTCCATGATTTATAATTGGCCAGGACAGCAGGTTTGTTGAACCTTGTAGCTACCATTTTGTTTCTCGTCCTTTCTTTTTTTTTTTTTAATAAGAAAATTTCATTGATGGTTTGAAAAAAACAGGAGAGAATTTGCACCATACCACAACCAAACTAACTATAGCTTCACAAAATCTAGAATAGGTAAGCTCCTTTTCTTGGAAGGTCTTCTATTTTGTTCAAGCCAAGTAGTCCAGTAGAAAGCTCTCACGAAATGTTCCCATAAAAGCTTCTTTACCCTTAATTTTCTTTAAAGAACTTTCATTGGGTAAAAATGAAAGAATAAAAGGGTATACGGAAAATCAAACCCACAAGAAAGGGAAACTAACTAAAAGAAGGGGCTCCAACTAGCAAAATAGAACTTATTGAATAATTACGAATGTTCTTTAAAATCAAAGTCTAAAGAGAAACATGAAATCTAACAAAGGGAAGCTTCTTTTCCTATTTAGAAGGATAACTGCCCAAAGTGTATGCAAGGTAAATCTTTGAGTCCCTCAGAAGTGCCATGAGCCAGATTTCTTCTAAGTTGTTGGTTCTAGCAGGTGTTTTTGACACTTCACATGTCTGAAATGGCTTCATCTTTGCACAGAGAGTTTAAAATAAGAGGAAACCTTTCATGAAAGAGAGCATCTCCAGCCAAACATTTTTCCAAAATGATAAACCCTTACCATTTGCAACAGTGTGGCTCATTCTGTCAAGGCTGATGGGGTGGTATTGTAATATGATCTTCTAGGGATCTTTAGCTTATCGTTGAGTGGTTTTTCTAGGTTCAGGAAACTTGTAGATGGACCCATATTTGGCGTCAATCACCTTCCCCTTCCACCAGAGGGCAGTTTTTTCATATTGATATCCCCAAATCCATTTTTCAAGGATTCTCTCTCTATTTTATATTTTAATACCGGATTCTTTTTCTTTCCCCCGAGTTTTAGTACATAATAGTATATTACTTTGCTAGAATTTATAGAACATACAACCAAGACTTCTTATACATTTTCAAATACAATAATTAACACGTTCGATTAAAGTTGAGAATTCTAGATTGTTCTCTTGCTTCATTTGTATTTGAGAATTGCGCTTAGCTGATTCTATCTTGTTATAATTCTATCATTGTAAATATCCTTGTTATATTTTCTTTTTGTGATTTTTCCTACACATTCCTTCTTTGGTCACCATTGTATCTTGTATATATATTTCTTTAGTGAATACAATAAGTTATTATGATTCTCTCTCAAACCTAAGTGTTATCACAGAGAAAATATGTACTTGACTTGTTGACTGAAACATGGAAGATAGGGGATAAGCCATGTAGTACCCAATGATGCCCAACTTACAGCTAACAAAAAATGGAGAATTGTTGAAAGATCCTGAAAGATATAAGAGGTTAGTGAGAAAACTTAATTACCTTACAATGACTTGACCCGACATAGCCTATTTAGTGAGTATTATGAGCCAGTGTATTCCATGCCCAACAGTCGATCATTCGGCTGCTTTGGAATGATTCAGTGTTATTTGAAGGTTGCTCTCGAGCATGGTTTATTATATAAGGATTATGGTCATACTAATATTGAATGTTTCTTAGACGCCGATTGGGCAAAATCTAAAGAAGACAGAAGATCAACTTCAGGATATTGTGTTTTTGTTGGTGGCAATTTGATGTCTTGGAAAAGTAAAAAGCAAAACATGGTGTCACATTCAAGTGTAGGATCAGAATATAGAGCAATTGGACACTCTAGTGTGAATCGATTTGGATATATCAACTAATTGTTGAGTTGAGATTTGAAATCACCACACCAACAAAATTGTGGTGTGGTAATCAAGTAGCATTTCATATCGCATCTAATCTGGTGTTCCATGAGAGGACTAAACATATTGAAGTTGATTGTCATTTTGTACATGATAAAATTCAACAAGTTTGGTATCTACAGGATATGTGAAGATTGGAGAACAGTTAGGAGATATCTTCATGAAAGCATGGAATGGAGCCCGTATAGATTATCTATGTAACAAGTTGGGCATGATTAACATGCATGCTTTAACTTAAGGGGAGTGTTATAATTCTATTATTGTAAATATCCTTGTTATATTTTCCTTTTTTGGTTTTTTCTACACTTTCCTTCTTTGGTCACCATTGTATCTTGTATACATATTTCTTCAGTGAATGCAATAAGTTATTCTGATTCTCTCTCAAACCTAAGTGTTTCACATTTCTAAATCATATCCCCCCTCCCAAGAATTTTGTGTAATTTGTGTTACATGTTGAGGACCTAGTTCAGTTTATTAGACAAGCTTGATGCTTCATTTATTTCCAGGACTATTGGTTAATGAAAATCTTCTGGTACTCTTCTTCAATCCTCTTTAAGTTGTTTAAGAGACCTTAGCTCGACACAATTTGTACTGAGATAATGGTCTATTAATTTATGAATTATATACAAAGCATAACAAATATTGTTTCTTTCAGCAATTAGATAATTATTATGCTTTATAGATATATATATATTTTCTTAAGTTCATTGTATTTTCCTCTTCCAACTGCAGGTTGACATGATTGTCCTGACAGATGGAAGTCGTATTCTTGGCCTCGGTGACCTTGGAGTTCAGGGAATAGGAATACCTATAGGAAAGCTAGATATGTATGTTGCTGCTGCTGGAATAAACCCTCAAAGAGTATGTTTTTTTCTTTTAATATTTGAGCTTGTTTATGACTGGCTCGTCCAAGCCTGTATGTGTTTTTGTCCCTACATTTATCCCTCTAATATCTAATCCTTCTTGGACTGATCGATGAACATGCAGTATACAGTGACGAGAGGTTTAATAGGTTAGAGTAGATTTTCATTTTTTATTTTGAAGATAAATAAGACCTCAATGGTTTGCAATTGCAGCCTATTGTAATTTTATCACTTAAATAACTATAAACATATACTTAAGTTAATTATTGTAAAAGATAAAATTATAATATGCTGCTATTGCAAATTATAGAGCTCTATTTGTCTTTAAAGGAAAAAAAAAGGATAAATTACACTTTTGATTTATGTGATTTGAGATTTGAGTCAAATTAACCCCTTGGTTTCAAACTTGAACAATCAAGTCTCCAACATTTGAAAATTGCTTAATTTTGGTCCCTTAATAACAGAGTTTGTAAAAGAATTAACTGTTTCCTGGCGTAGCATTTTTAAAATAAAAAACAAGTTATTATAATAATTATAATTTAAATAAAAGTAAATAAAATATTTCTCTTCTCTAACCCTTCTCCAAACCGAGGGATCAGAAGTGTATTTTGCCGTATTATTTATCTTAATAAACATGTATATATTTTTATCTTTATTCCTTGAAAATTCTCAAGTTAATGACCCCATTAAGAGATAGCTTGAAATTAAGCATTTTGTAATTGTTGGGGACTTGATTGTTCAATTTTGAACTTTAAACCTTAGAGACCAATAATTGTAGTCTATCCAAAAATTATGTGTAGTTTAATTTAATGAATGCTATGTAGTTTGTGTTTCAAACAAAATTCTAAAAATTAAACCACAAACATGATGAGGATGCTAAGGGGATGTCAACCTAGTTGAGATGTCCGAGTGCGTCTGCCAATCCTTAGTTCTTAATTGCTCATTGTATAACCCTCTTGTACTTTGAGCTATGCCTCATTTTTTATAATAAATGAGATTCGTTTCCTTTTAAAAAAAGGGGAGAACCCAACCTCTTCTTCACCTTTGATGAACCCAAAGCATTTTGCAACCCATACAACCAACAACAAACTAAAGACAATTTTCGCTCTGAAGTTAAGCGGTCTACAGCCATTGTGCCTTTGCTTTTTTATGTTGATATGGAAAATTACCTTTCCAACCCCATCCCACTCCGCTTCACTCTTTTCATCCATCTTCCATAGTTGAGCCTTTTGAACCACAATGGTATGATTTCATCCCTCCTAAAAAAACGAGCCATTCAATCCAAGGCCTCATATAATTGCATTGGCTCTGGTTCAGAGTTCGATTCTGTATTATGGCTATCCCCACTAAGAAAAAGAACGAAAACAAAGCATTCGATGAAACAAAGGAAAGGCATTAGAGAAATTCAGAACTTGAAATGCTCAATCTTTTATGAGAAAAAAAGCTACTTCTTTGCTTAGAGAGGGGTCAATACCGACTTGATCCTTTACAAGCTCAACGCTCTTCCTTCATCAAATGGTTGTTTATCCAACTTGGTTTCAATCAGTTGTGGCTATTGTTTTAATTGTTTATAGGATCACCTGCATTCAGATTGTTTTATCTTCTCGTATTGGGGTACCTCTAATCAATGTTCTTTGGATTGTTGTTTTGTTTGGTTTGCCCTTTCGTTTGCAATAAGAGATTGGAGGGCTTCTTGGACCGTCAAGCCAGCTTGCATCTCTATGTTTTATCCCTCCTTTTTGTTGCCCCCCTCTCCCTTGATTTTGTTGTATCTATATCTTTTACTTGGGGTTTTGTTGTATTTCTTTATGGGGCTTTCCTATTGTATCATTTTGGATATTTCATTTATCAATAAAATTGTTTCTTATCCCCCCAAAAAACATGCTTAAATATAGAGGTATAGTTTGCAGATTTCACGAGTAGTAGGTTTTGTTATTATTATTTTTTTTTTGGGACCATGTAGTCATGTATTATAGCTGGCCTCTTGTTGGAGTGTCTACACGAAATGTTCTTTTGATTGGATCTCTTGGGGTGGGGGGGGGTCCCCTCCCCTACTATTTTCCTCTTTTGTCTCTTCTGTGGCATTAACTAAATAAATTATTGAAGTGTAGACTTGAATTATTCCTTATTGTGTCAATCTATATTTATGTTACTTTTTGGATAAATTTCTAAAGGAAATTTGGTTTGTTTTGGTGAAAGAGGCACTGTCAGCATTGACATGTACATGTGATTTTAAGCAAGAACCAGGAGAGTGACAGTTTTAGTGTGCCGTGTTTAGAATTCGCCGATATCTTTTTCCCGTTCCAGCAAGTATTCCTGATAGTGGGGGTAACCCTCAAGTCTAGTCTTTTCTTTATGAAATTTGTAGATTACCTAAAAATAAATATCATTTAATGATGACATTTTTTTAACAACTTTGCTTAACCCCACTTTCTTGAAATTTCATTAAGTTCACTCTTGTTTAATTGACAGATTCTTCCAGTAATGCTAGATATTGGCACTAACAACGAGAAGTTACTTAATGATCGTCTTTGTAAGTGAAATAGAAACCTTCATGTCTTTTATCTCCATATTTCCTTGTAATGTATTGTGCTGGTATTGCTCTGTTTCTGTTGTACAATGGCGATATATCTGGGCTTTTTGTTGTTGTTGTTGTTGTGGTGGTGGTGGTGGTGAAATATTTTATTTAGGCCCCTAGTTAATATTTAGTTTGCTTAACAGAAAATTTGTTGCTTGAGCTTGCTCAATTTCATCCTTTTACTTGTTCAGGTCCTCCTCTAGCCTTTGTCATCTCCCTATAGAATATGCAAACACTTGTAGTATGCAAGATTTGCAGATATATGGAGCCAGTTACTGGTTGTATGTTTTTAGAAAGTTCCAAAGTGTGATATCCTTTGTTTCTCTAAAGAAAAATTTGAAACTTCCAAAATGTTTAAATATGGGACCTCAACATATTTGGGTTTATGGTTCCTGCTTTTAGGGTGCATTGGTTTGATTTTCATTGGATTGTTGTTTTCTTTAGTTTTCCCCTTAATTGTGAGTTTCTTGCATTGTCAATCCATTTTACATCCATTTTAAGCTTATAAACACTACTTCCACCTATTGATTTCTGTCTTGTTTTCTAGTTTTGAAAAACCAAGCTAGGTCTGAAAAAGATTAAAAGAAAATAAAAGAAAGAGTTCAAACTTGTTTTTGTTTTTACGATTTGGCTCAGAACTCAATTGTTTAGTTAGAAAAGATGGAAACAGTAATTGCTTAAAAACAAAAAACAAAAATCAAGTGGGCCTTAGTTATTGGGGTGTCTCCTAAGGTTCAAATTTTTCTTTTTCAATTTGAACGGTCTTTGGTGTCTATTTGCCTGCACTTTGTTAAATGGTCTGTATAGTGTTCTTTAAAGCTCTAGACGCATCTTTATCTTTTTTTTGGGTGAGAGGCACTATGTAAGAGGACTGCATAAAGAAGGGAAAGTATAGTTGAAGTGGAATTATAAAGGAATACCCTAAATTCAAAGAATTTTGCATTTAGGCTTAGAAAATTTTAATTTAAATTATTAATAAAGGAGGGAAAATCCTAATTTTTTTTTTTTTTTGGGTGCACACCATATTTTATGGGCCTCATCTTGAGGTGCCAGACATGCACTTAACGCCTAGGCATGGCCCGAGATGGTTTGTTAAACATTGAGTCTGCATTATTTCTTCTAGACGTTTCATAGAATGTCACGTCACATGATCTAGTATGGCAGATTTAGGACTTCGGCAACCTAGATTGGAGGGAGAAGAGTATCTATCAATTGTTGATGAATTTATGGAAGCCGTGCATACACGTTGGCCCAAAGCTATTGTTCAGGTTTATTGGTTAAGATGTCAAATTTTTAACTTAGTGTACACTGCCAGTAATCAGTATCGTAACCTCGCAATTTGATCCTGAATGATGCCAGTTTGAGGATTTTCAAATGAAATGGGCTTTTGAAACATTACAACGTTATCGTAAGAGGTTCTGCATGTTCAACGATGACATACAAGTGAGTAGTTGATGCTGTATTCAGGCAGTAGATAATTACTTAAAAGGACATTCATTTTCATTTATTTCATTTCATAAAGGGAACTGCTGGTGTTGCTCTCGCTGGACTATTGGGAACTGTGAGAGCTCAAGGGCGGCCATTGAGTGATTTCGTCAATCAAAAGATAGTAGTGGTAGGGGCTGGAAGGTATTGTCAGCTGTTTAACTTTAACATTTACTCCAAATAGTAATACTGCTTACGATTTAGGGATCATAAGCCTTTTCCTCTTTGTTGAAACAAAAGTGGTTGTTTGAAAGAGGAATTACTATTACTATTACTATTACAATTACTCTTACTATTACTATTACTATTACTCTTACTATTACTGTTACTATTACTATTACTCTTACTATTACTGTCACTGTTACTATTACTATCACTATCACGTGAATATAAGAGGAATGAAAACTTTAGAAATTATAGTTCAATCCTTTTGGTTTAACCTTCATTTGTCTCAACATGCTGTTTTTTCCCCTCTTTCGACTTCAGTGCAGGGCTCGGTGTTCTTAACATGGCTATTCAGGCTGTTTCGAGAATGGCAGGGAACAACGATTCTACTGCAAGAAATCAATTTTTTCTAATAGATAAAGATGTAAAAATCTTTTTCAAACTTTCATTTATCAATTTCTGCATCAGTCTTGCTAAGTTTTTCCTTCCATGAGAGTTATTGTACAAATTGTATATTCATTTCATGGATTTGGCATGATCCGTGATCATGCTGCAAATTGACCTCATCTATCTGGCCAATTGTCTTTGTAGATTACAAATTACATATTAATTGAAAAAGAAAATTATCATTTCTTTTAACGCTATGTAATGGTAGTCAAGTATAGTAGTCATGCCTCTACTCTAGCTTATATATTCATTTTTGCCTATGTGACTTCGACACTTTCATTTATTAATTTCATGAAGTCAGTGGGTTGATTTTAGATTGCTAGCATGATCGTACTGCTGGGAAAGCTTAACATTAAGCTTGTTTTGTTTACGTTTGAACTACTCAAATTTCTAGCATGTAAAAATTATCATTCATGAACACGATAAAGCAGATTTTAAAGATGTGGAAAATAAATTTTTGTTTAATATTTGATTGAACGGGAAATGAATTGTTTGTCATTATTGTTGTCTTGCCTTTTCTACTACTGTATCTTTTAGGTAAATTTCTTTCTTGTAATACTTTTTTACTTTAATTTTAATTAATTTATTTTTCTGTGAAGAATGCTCTTCCTTGTTCTCAATTTTTATCTACATCATATTTGTTTTCCTTACATCCGATCCCAAAAAGGTCAACTGTTGTGCAAGTGATATATTAGAATATTAATTTCTTATGAAATTGACATTGATCTTAACTTTTCAGATCTATTGTTGGTTTTTTAATCTTTATGAAGTGAATTTAATTGTTATATGCTCTTCACACCTTATTGTCATGAATCATGATAATGCCTGTGATGCAATGAATTTTAGCAAGGTTATGCTGCTAGATTTTTAACTCCATGTTACATGTGTCATCTGAGCAAAAGAAATTATGTCTTTGTGCAGGGCCTTATCACAAAAGAGAGGACAAACATTGATCCAGCAGCTGTACCATTTGCTAAAGATCCAAGAGAGCTCGAGGGACTTAGTGAAGGAGCCAATTTATTAGAAGTGGTAAGCTTTCTCAAACTCCCTTCATGGTTAGGGTTACTTAGCTAATTTCCTCAACACTCAGAGAGGATTCTGCCATTACTCTTCAGTAGTTTTCGATCATCTCCATTGAGATTTATGGATGAAATTTTAATCACTGTTCAGGTGAAAAAGGTCAGGCCCCATGTTCTTCTTGGTCTGTCAGGAGTTGGCGGTATCTTCAATGAGGAGGTACATATCCTCTTTTTTTCGTTAGTTGGGGCTTACTTCAACTTCCAAAAAGTATTATCTCAACAACATCAATATGTATGATGTTTCGGTTATTTTCATTTATGCCACATGTTTTTAAGTCGTCCATGCTTTTATCTCACACTTTCTTGTTATAAATTTGAAAAATAAGCAACTTAAGAAATCCATTTCTCATTCATCTTTTTCTCTGGAGTGGAGATTATTTTAGACGTCTCCTCAGATAAAAGCAATGATGGAGGATAGGAGAAAGGAAATTCAATATCTCTCTAGTAAATTACTTGCATCATTGTCTTTTATTCATTTAGCCTCATAATTACGAGGACAGAAGTAATGAACTTCAACCTCATTGTCCTTTTCCATTTAGTTAATGGTGAACTCAGTGTAGGATGGTGTCAATTTTCGGGTTCACTTTCCTCTTCTAGTTCTCATATTTGATGGGATTACTCTAGGTGCTCAAGGCAATGCGGGAATCTGATTCAAGTAAACCTGCAATATTTGCAATGTCAAATCCCACCATGAACGGTTTGTTTGTTTATTAGGATGCAATAATTTTCATATTAGCTAGCTATTTGCAGTTACAACTAGTTATCGGTTTTTTCTTTTACCAGCTGAATGCACTGCTGCTGATGCTTTCAAGTATGCTGGAGAAAACATTGTCTTCGCTAGTGGAAGCCCTTTTGAGAACGTTGCTCTTGGTATCTTCTATGTTTTGAAAAATAAAATGTTTGAAATGGCATCTACCTAAACGATCAATTTAAAGTTTTCCTATGGGTTGCACGAGCTTTGTAATTTTCGGACTCTAGCCCTGTATCTTATAGTTTAATATGACTTTCTGTAAAATAACTTGCAGGTAATGGAAAGTTTGGCCATGTAAATCAAGCAAATAACATGTATCTGTTTCCCGGGTTTGTATATTTCCTTTAAAAACTGTTATTTTGAACTTGTAAGAATTCGGTGAGTTAATTGTTTAACTGTGATTGTTTTCATGCAGGATTGGGTTGGGATCTCTTCTATCAGGTGCTCGTTATATTACCGATGGAATGTTGCAAGCAGCTGCAGAATGGTATGAATCTTTATGTAGCAGTCCAATAACATCTCTTTTTTTAATTTTAAATTTGGAAGACCGAAGATGTTAAAAATGCCTAGTTTGCTGCGGAAACCTTAGGGTGTATTGAGAGGACTTGTCTTATGGTTGTTGGTGAAACCACTTGCAGACTGCTTATGAAAAAAATGATAAGTGGGGTTGTATCTCTTTCCGTACAGCGTCCAATAGTTTTACTCTAAAAGTATAAACTCTTTACTCAAAATTTTCCATACAAACATCCAATAGTTCTTTCTTAATGAAAGCAATCTTTGTAAATTTGATTAAGCCTCATCTAGGCATATAAACAATTCTTCCACCTCTAAATATCTTGCTTTGCTATCCAACTTCTACCAATGTTTTTATTTTTTAAAAATTAAGCCAACTTTTGAAAATTAAAAGAAAAATAGTTTTTTAACTTTTTTTTTTTTTAAATTTGGCTAAAAATTAAACTCTTGTGGAAAGATGTCAATCAATGTATAAGAAAGATGCAAATCAATGTAAGAATAGAGGAAATAGGCTTAATTTTGAAAAACTAATAATTAAAAAACGAAATGGTTACCGGTTGAGATCTTAGGCCTCGTTTGTAACCGTTTCATTTTTTGTTTTTGGTTTTTAAAAATTAAGCTTTTCTCATTTTCTTACCATGGTTTGTATCAATCTTAAGTAGAAGAGTTGCATTCTTAGCCAAATTTAAAAAACAAAAACAAGTTTTTAAAAACTACTTTTCTTAGTTTTCAGGTTTTTTAAAATTAAGCCTATAAACATCTCACACCTCAAAATTTCTTGTTGTGTTATCTATTTTCATTCAATGTTTTAGAAAACCAAGCTAAGTCTTGCAAATTAAAAAAAAAAAAAAGGTAGTTTTTGAAAACTTTATTTTCTGTTTTCTATTTTGAAATTTGGCTGTGAATTCTACCATTCTACTTAAATAATGAAAATTATGTTAAGAAATTTGGAGGAAATAGATTTTATTTTTAAAAACCAAATGATTATCAAATGGGGTCTAAAAGTAGATAATTTTAGCTCCTTACCATTTTGACACTATCCTTGATTTATGTTTTATTTATCTCCAAGGTGCTGATATGATGTTGTCATCTATGCAGCCTTGCTTCTTATATGACAGATGAAGAAGTTCAAAGTGGCATTTTGTACCCATCGATTGATAGGTAGGAGTAGGATTCACGGTTCTATATTCCATCATATTGTCATATAATTGAATCAGCACAATCTGATTACTTCATTGTTTAGACTGGTTGACCAAAGAACTAACCTCCAAAATATGTAAATACAATTTGCAGCATTCGAGAAATCACAGCAGAGGTCGGAGCTGCAGTCCTGAGGAGTGCTGTTTCGGAGAATTTGGCAGAAGGCCATGGCGATGTGGGCCCTCGAGAGCTGAGGCTAATGGCCAAAGTACGTAAACTGATCTCATGGTTTGCATATTATTCGTCTGTGTTTCTAAATTATATTTTCAAATCTCCACTCACTCGAGAAAATAAAAAATAAAGTAAACCTCATATGGTGCATAAGGTTATAAGCACCTTCTCTTTTTTCCTCTTCCTTAAAGTAAGGTACCCATCTTAGAGTAAATGACTTTTGTCTGTGGACAGTGAACTGATAATTGAGGATAGATACAGCTTTAACTTAAAGACTGGAGATTGCAAAAAATGCCTACATTATTATTTGGGTTAAAAACGAAGGAACCTGTCTAAACTCTAGTTTCCATCTTCTCGTTTATTTATAGAGTGACGATTAAATAATGTTATTTCTTCTGATCATGAATCTGTATGCAGGGATTTCAACGGGCACACACTTTTGTTTTTATTTTCATAATTCATTCCGTCACAAGTTTCAGAAAACAGTTTGTTACCTTTGATAATTATCAAATATATAACCGCATGGGAATCTGGCTGTTGCTAAAATTTCTGTAATTTCTTGAGTTTATTGTTGAAGTTTATTGCTTTTTCTGCAGGAGGAGACGATTGAATACATCAAACGCAATATGTGGTTTCCGATTTATAGCCCTCTTGTCCATGAAAAATAAGCCTCATTTAGAGCTCTACCATTCTTTTCTTTTCTCCCAATCGTCATCTCTCTTATAGCTCGGGGTCGGGGCCAAACAATTGAACATAAGGAACTAGGTCTCTCTATTCTGAAGAACTCTGAGAAACGTCCTTGCTTGGATTCCCTCAGGTTATATATATATATATTCTCAATTATTGTATTCAAGGTGATTAACATCATCATGCAATTGGAGTAGGGAAGTTTAGAGTTCATATTTGCAGCCATCCATTCTCTTTATACTTCCATTATAAGTTCTTATCCGAATTTTTTGATATCACAACAGACTTTTCTTTAGTTTTCTTTCTCCTTCCTAATTTCATATCTGAGAGCTTTGCTCAAAAAAATTAAATTTAATTATCGTTTAGTTTCTTCA

mRNA sequence

CGCCTCTCCGCAATTATTCCTCCATTATTCGTCAACAGCTTCAACTGCCCCGCTATAATTTGACCGCAACTTTTATGTTTCAACTGAACAAAGCCTCTGAAACCATTACAACCTTAACTAGCATTGCTTCGAATTACAGCGTCAATCCCGCGAACTTTGGGATCTTTTTTTTAATCTTTTTGGAAGATTGGTTGGACGCTTGAAGTAGTAGAGCACTGGATTTGCGGCACTGCTGCGTTGGGTTTACTGGTAGCCAGATTGGATTTGGTTTCTGCTTTCTTGATTTTTCCCTAGATCCAGCTTTATGTGGAACCTTGTGAAGTTTACTGCAGCGAGTTTTCGCCGATCTAGGCGGTTGTCGACTGCGATTCCTGGTCCTTGTATTGTCCATAAGCGCGGCGCTGATATTCTACACGATCCTTGGTTCAACAAGGATACTGGATTTCCTCTCACTGAACGAGATCGTCTAGGAATCCGTGGCCTTCTTCCTCCACGAGTAATATCATTTGAGCAGCAATATGCTCGTTTCATGGAATCATATCGGTCGCTGGAGAGGAATACTCATGGTCAACCAGATGGCATTGTTTCATTAGCGAAATGGAGGATTTTAAACAGATTGCATGATAGGAATGAGACACTGTACTATCGAGTCCTTATTGATAACATAAAAAGCTTTGCTCCAATAATATACACACCTACAGTGGGATTAGTTTGTCAAAACTATTCTGGGTTATTCCGACGTCCACGTGGAATGTATTTCAGTGCAAAGGACAAAGGAGAAATGATGTCCATGATTTATAATTGGCCAGGACAGCAGGTTGACATGATTGTCCTGACAGATGGAAGTCGTATTCTTGGCCTCGGTGACCTTGGAGTTCAGGGAATAGGAATACCTATAGGAAAGCTAGATATGTATGTTGCTGCTGCTGGAATAAACCCTCAAAGAATTCTTCCAGTAATGCTAGATATTGGCACTAACAACGAGAAGTTACTTAATGATCGTCTTTATTTAGGACTTCGGCAACCTAGATTGGAGGGAGAAGAGTATCTATCAATTGTTGATGAATTTATGGAAGCCGTGCATACACGTTGGCCCAAAGCTATTGTTCAGTTTGAGGATTTTCAAATGAAATGGGCTTTTGAAACATTACAACGTTATCGTAAGAGGTTCTGCATGTTCAACGATGACATACAAGGAACTGCTGGTGTTGCTCTCGCTGGACTATTGGGAACTGTGAGAGCTCAAGGGCGGCCATTGAGTGATTTCGTCAATCAAAAGATAGTAGTGGTAGGGGCTGGAAGTGCAGGGCTCGGTGTTCTTAACATGGCTATTCAGGCTGTTTCGAGAATGGCAGGGAACAACGATTCTACTGCAAGAAATCAATTTTTTCTAATAGATAAAGATGGCCTTATCACAAAAGAGAGGACAAACATTGATCCAGCAGCTGTACCATTTGCTAAAGATCCAAGAGAGCTCGAGGGACTTAGTGAAGGAGCCAATTTATTAGAAGTGGTGAAAAAGGTCAGGCCCCATGTTCTTCTTGGTCTGTCAGGAGTTGGCGGTATCTTCAATGAGGAGGTGCTCAAGGCAATGCGGGAATCTGATTCAAGTAAACCTGCAATATTTGCAATGTCAAATCCCACCATGAACGCTGAATGCACTGCTGCTGATGCTTTCAAGTATGCTGGAGAAAACATTGTCTTCGCTAGTGGAAGCCCTTTTGAGAACGTTGCTCTTGGTAATGGAAAGTTTGGCCATGTAAATCAAGCAAATAACATGTATCTGTTTCCCGGGATTGGGTTGGGATCTCTTCTATCAGGTGCTCGTTATATTACCGATGGAATGTTGCAAGCAGCTGCAGAATGCCTTGCTTCTTATATGACAGATGAAGAAGTTCAAAGTGGCATTTTGTACCCATCGATTGATAGCATTCGAGAAATCACAGCAGAGGTCGGAGCTGCAGTCCTGAGGAGTGCTGTTTCGGAGAATTTGGCAGAAGGCCATGGCGATGTGGGCCCTCGAGAGCTGAGGCTAATGGCCAAAGAGGAGACGATTGAATACATCAAACGCAATATGTGGTTTCCGATTTATAGCCCTCTTGTCCATGAAAAATAAGCCTCATTTAGAGCTCTACCATTCTTTTCTTTTCTCCCAATCGTCATCTCTCTTATAGCTCGGGGTCGGGGCCAAACAATTGAACATAAGGAACTAGGTCTCTCTATTCTGAAGAACTCTGAGAAACGTCCTTGCTTGGATTCCCTCAGGTTATATATATATATATTCTCAATTATTGTATTCAAGGTGATTAACATCATCATGCAATTGGAGTAGGGAAGTTTAGAGTTCATATTTGCAGCCATCCATTCTCTTTATACTTCCATTATAAGTTCTTATCCGAATTTTTTGATATCACAACAGACTTTTCTTTAGTTTTCTTTCTCCTTCCTAATTTCATATCTGAGAGCTTTGCTCAAAAAAATTAAATTTAATTATCGTTTAGTTTCTTCA

Coding sequence (CDS)

ATGTGGAACCTTGTGAAGTTTACTGCAGCGAGTTTTCGCCGATCTAGGCGGTTGTCGACTGCGATTCCTGGTCCTTGTATTGTCCATAAGCGCGGCGCTGATATTCTACACGATCCTTGGTTCAACAAGGATACTGGATTTCCTCTCACTGAACGAGATCGTCTAGGAATCCGTGGCCTTCTTCCTCCACGAGTAATATCATTTGAGCAGCAATATGCTCGTTTCATGGAATCATATCGGTCGCTGGAGAGGAATACTCATGGTCAACCAGATGGCATTGTTTCATTAGCGAAATGGAGGATTTTAAACAGATTGCATGATAGGAATGAGACACTGTACTATCGAGTCCTTATTGATAACATAAAAAGCTTTGCTCCAATAATATACACACCTACAGTGGGATTAGTTTGTCAAAACTATTCTGGGTTATTCCGACGTCCACGTGGAATGTATTTCAGTGCAAAGGACAAAGGAGAAATGATGTCCATGATTTATAATTGGCCAGGACAGCAGGTTGACATGATTGTCCTGACAGATGGAAGTCGTATTCTTGGCCTCGGTGACCTTGGAGTTCAGGGAATAGGAATACCTATAGGAAAGCTAGATATGTATGTTGCTGCTGCTGGAATAAACCCTCAAAGAATTCTTCCAGTAATGCTAGATATTGGCACTAACAACGAGAAGTTACTTAATGATCGTCTTTATTTAGGACTTCGGCAACCTAGATTGGAGGGAGAAGAGTATCTATCAATTGTTGATGAATTTATGGAAGCCGTGCATACACGTTGGCCCAAAGCTATTGTTCAGTTTGAGGATTTTCAAATGAAATGGGCTTTTGAAACATTACAACGTTATCGTAAGAGGTTCTGCATGTTCAACGATGACATACAAGGAACTGCTGGTGTTGCTCTCGCTGGACTATTGGGAACTGTGAGAGCTCAAGGGCGGCCATTGAGTGATTTCGTCAATCAAAAGATAGTAGTGGTAGGGGCTGGAAGTGCAGGGCTCGGTGTTCTTAACATGGCTATTCAGGCTGTTTCGAGAATGGCAGGGAACAACGATTCTACTGCAAGAAATCAATTTTTTCTAATAGATAAAGATGGCCTTATCACAAAAGAGAGGACAAACATTGATCCAGCAGCTGTACCATTTGCTAAAGATCCAAGAGAGCTCGAGGGACTTAGTGAAGGAGCCAATTTATTAGAAGTGGTGAAAAAGGTCAGGCCCCATGTTCTTCTTGGTCTGTCAGGAGTTGGCGGTATCTTCAATGAGGAGGTGCTCAAGGCAATGCGGGAATCTGATTCAAGTAAACCTGCAATATTTGCAATGTCAAATCCCACCATGAACGCTGAATGCACTGCTGCTGATGCTTTCAAGTATGCTGGAGAAAACATTGTCTTCGCTAGTGGAAGCCCTTTTGAGAACGTTGCTCTTGGTAATGGAAAGTTTGGCCATGTAAATCAAGCAAATAACATGTATCTGTTTCCCGGGATTGGGTTGGGATCTCTTCTATCAGGTGCTCGTTATATTACCGATGGAATGTTGCAAGCAGCTGCAGAATGCCTTGCTTCTTATATGACAGATGAAGAAGTTCAAAGTGGCATTTTGTACCCATCGATTGATAGCATTCGAGAAATCACAGCAGAGGTCGGAGCTGCAGTCCTGAGGAGTGCTGTTTCGGAGAATTTGGCAGAAGGCCATGGCGATGTGGGCCCTCGAGAGCTGAGGCTAATGGCCAAAGAGGAGACGATTGAATACATCAAACGCAATATGTGGTTTCCGATTTATAGCCCTCTTGTCCATGAAAAATAA

Protein sequence

MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQFFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLVHEK
Homology
BLAST of PI0019452 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 9.9e-298
Identity = 500/604 (82.78%), Postives = 551/604 (91.23%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MW + +  A++FRR+RRLSTAI  PCIVHKRGADILHDPWFNKDTGFP+TERDRLG+RGL
Sbjct: 1   MWRVARSAASTFRRTRRLSTAISAPCIVHKRGADILHDPWFNKDTGFPMTERDRLGLRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQY RFMES+RSLE+NT GQPD +VSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYDRFMESFRSLEKNTEGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMI+NWP  QVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIFNWPSTQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQR+LPVMLD+GTNN+KLL D LYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPVMLDVGTNNQKLLEDPLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEF+EAVH RWPKA+VQFEDFQ KWAFETL RYRK+FCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFVEAVHARWPKAVVQFEDFQAKWAFETLDRYRKKFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPL+DF NQKIVVVGAGSAGLGVL MA+QAVSRM G    +A   
Sbjct: 301 GVALAGLLGTVRAQGRPLTDFANQKIVVVGAGSAGLGVLKMALQAVSRMTG---PSADPH 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELE--GLSEGANLLEVVKKVRPHVLLGLSGV 420
           FFL+DK+GLITK+R +IDPAA+PFAK   E+E  GL EGA L EVVKKV+PHVLLGLSGV
Sbjct: 361 FFLLDKNGLITKDRKDIDPAALPFAKAHHEIEGLGLQEGAGLAEVVKKVKPHVLLGLSGV 420

Query: 421 GGIFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVAL 480
           GGIF+EEVL+AM+ESDS +PAIFAMSNPT NAEC   DAFK AGE+IVFASGSPF NV L
Sbjct: 421 GGIFHEEVLRAMKESDSVRPAIFAMSNPTNNAECCPVDAFKLAGEDIVFASGSPFANVDL 480

Query: 481 GNGKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYP 540
           GNGK GHVNQANNMYLFPGIGLG+LLSGAR I+D ML+AAAECLASYM+D+E+  GILYP
Sbjct: 481 GNGKIGHVNQANNMYLFPGIGLGALLSGARNISDTMLEAAAECLASYMSDDEINRGILYP 540

Query: 541 SIDSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSP 600
           SID IR+ITAEVGAAVLR+AV+E+LAEGHGDVG +EL+ M+KEETIE++++NMW+P+Y P
Sbjct: 541 SIDDIRDITAEVGAAVLRAAVAEDLAEGHGDVGVKELQHMSKEETIEHVRQNMWYPVYGP 600

Query: 601 LVHE 603
           LVHE
Sbjct: 601 LVHE 601

BLAST of PI0019452 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 3.0e-294
Identity = 493/596 (82.72%), Postives = 545/596 (91.44%), Query Frame = 0

Query: 9   AASFRRSRR-LSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVIS 68
           AA    SRR  STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRV++
Sbjct: 15  AARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMT 74

Query: 69  FEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPI 128
             QQ  RF+ES+RSLE NT G+P+ +V+LAKWR+LNRLHDRNETLYYRVLIDNIK FAPI
Sbjct: 75  CVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYYRVLIDNIKDFAPI 134

Query: 129 IYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLG 188
           IYTPTVGLVCQNYSGL+RRPRGMYFSAKDKGEMMSMIYNWP  QVDMIV+TDGSRILGLG
Sbjct: 135 IYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDMIVITDGSRILGLG 194

Query: 189 DLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEE 248
           DLGVQGIGIPIGKLDMYVAAAGINPQR+LP+MLD+GTNNEKLL + LYLG+RQPRLEGEE
Sbjct: 195 DLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDLYLGVRQPRLEGEE 254

Query: 249 YLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGL 308
           YL I+DEFMEA  TRWPKA+VQFEDFQ KWAF TL+RYRK+FCMFNDD+QGTAGVALAGL
Sbjct: 255 YLEIIDEFMEAAFTRWPKAVVQFEDFQAKWAFGTLERYRKKFCMFNDDVQGTAGVALAGL 314

Query: 309 LGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQFFLIDKD 368
           LGTVRAQGRP+SDFVNQKIVVVGAGSAGLGV  MA+QAV+RMAG ++S A   F+LIDKD
Sbjct: 315 LGTVRAQGRPISDFVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISESEATKNFYLIDKD 374

Query: 369 GLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVL 428
           GL+T ERT +DP AV FAK+P E+    EGA+++EVVKKVRPHVLLGLSGVGGIFNEEVL
Sbjct: 375 GLVTTERTKLDPGAVLFAKNPAEIR---EGASIVEVVKKVRPHVLLGLSGVGGIFNEEVL 434

Query: 429 KAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVN 488
           KAMRESDS KPAIFAMSNPT+NAECTAADAFK+AG NIVFASGSPFENV L NGK GHVN
Sbjct: 435 KAMRESDSCKPAIFAMSNPTLNAECTAADAFKHAGGNIVFASGSPFENVELENGKVGHVN 494

Query: 489 QANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREIT 548
           QANNMYLFPGIGLG+LLSGAR +TDGMLQAA+ECLASYMTDEEVQ GILYPSI++IR IT
Sbjct: 495 QANNMYLFPGIGLGTLLSGARIVTDGMLQAASECLASYMTDEEVQKGILYPSINNIRHIT 554

Query: 549 AEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLVHEK 604
           AEVGAAVLR+AV++++AEGHGDVGP++L  M+KE+T+ YI RNMWFP+YSPLVHEK
Sbjct: 555 AEVGAAVLRAAVTDDIAEGHGDVGPKDLSHMSKEDTVNYITRNMWFPVYSPLVHEK 607

BLAST of PI0019452 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 4.5e-234
Identity = 399/605 (65.95%), Postives = 482/605 (79.67%), Query Frame = 0

Query: 6   KFTAASFRRSRRLSTAIPG--PCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPP 65
           +  AA    S R  T   G  P IVHKR  DILHDPWFNK T F  TERDRL IRGLLPP
Sbjct: 21  RVAAAVNSSSSRNFTTTEGHRPTIVHKRSLDILHDPWFNKGTAFSFTERDRLHIRGLLPP 80

Query: 66  RVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKS 125
            V+SFEQQ ARFM   + LE      P     LAKWRILNRLHDRNETLYY+VL++NI+ 
Sbjct: 81  NVMSFEQQIARFMADLKRLEVQARDGPSDPYVLAKWRILNRLHDRNETLYYKVLMENIEE 140

Query: 126 FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRI 185
           +API+YTPTVGLVCQ YSGLFRRPRGMYFSA+D+GEMMSM+YNWP  QVDMIV+TDGSRI
Sbjct: 141 YAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRI 200

Query: 186 LGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRL 245
           LGLGDLG+QGIGI IGKLD+YVAAAGINPQR+LPVM+D+GT+NE LL D LYLGL+  RL
Sbjct: 201 LGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTDNENLLKDPLYLGLQDHRL 260

Query: 246 EGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVA 305
           +GEEY+ ++DEFMEAV TRWP  IVQFEDFQ KWAF+ LQRYR  + MFNDDIQGTAGVA
Sbjct: 261 DGEEYIEVIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDIQGTAGVA 320

Query: 306 LAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNND---STARNQ 365
           +AGLLG VRAQGRP+ DF   KIVV GAGSAG+GVLN A + ++RM GN +    +AR+Q
Sbjct: 321 IAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARMLGNTEIAFESARSQ 380

Query: 366 FFLIDKDGLITKERTNIDPAAVPFAKDPRELE--GLSEGANLLEVVKKVRPHVLLGLSGV 425
           F+++D  GLIT+ R N+DP A PFA+  +E+E  GLSEGA L EVV++V+P VLLGLS  
Sbjct: 381 FWVVDAKGLITEARENVDPDARPFARKIKEIERQGLSEGATLAEVVREVKPDVLLGLSAC 440

Query: 426 GGIFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVAL 485
           GG+F++EVL+A++ S S++PAIF MSNPT NAECT  +AF   GENI+FASGSPF++V L
Sbjct: 441 GGLFSKEVLEALKHSTSTRPAIFPMSNPTRNAECTPEEAFSILGENIIFASGSPFKDVDL 500

Query: 486 GNGKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYP 545
           GNG  GH NQANNM+LFPGIGLG+LLSG+R ++DGMLQAAAECLA+Y+T+EEV  GI+YP
Sbjct: 501 GNGHVGHCNQANNMFLFPGIGLGTLLSGSRIVSDGMLQAAAECLAAYITEEEVLKGIIYP 560

Query: 546 SIDSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSP 604
           SI  IR+IT EV AAV++ A+ E+LAEG+ ++  RELR + + +  E+++ NMW P Y  
Sbjct: 561 SISRIRDITKEVAAAVVKEAIEEDLAEGYREMDSRELRKLDEAQISEFVENNMWSPDYPT 620

BLAST of PI0019452 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 3.6e-231
Identity = 383/583 (65.69%), Postives = 475/583 (81.48%), Query Frame = 0

Query: 25  PCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLER 84
           P IVHK+G DILHDPWFNK T F +TER+RL +RGLLPP V+  EQQ  RFM   + LE 
Sbjct: 39  PTIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEE 98

Query: 85  NTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLF 144
                P    +LAKWRILNRLHDRNET+YY+VLI+NI+ +API+YTPTVGLVCQNYSGLF
Sbjct: 99  QARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLF 158

Query: 145 RRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMY 204
           RRPRGMYFSA+D+GEMMSM+YNWP +QVDMIV+TDGSRILGLGDLGV GIGI +GKLD+Y
Sbjct: 159 RRPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLY 218

Query: 205 VAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRWP 264
           VAAAGINPQR+LPVM+D+GTNNEKL ND +YLGL+Q RLE ++Y+ ++DEFMEAV+TRWP
Sbjct: 219 VAAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWP 278

Query: 265 KAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQ 324
             IVQFEDFQ KWAF+ LQRYR  + MFNDD+QGTAGVA+AGLLG VRAQGRP+ DF   
Sbjct: 279 HVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKM 338

Query: 325 KIVVVGAGSAGLGVLNMAIQAVSRMAGNNDS---TARNQFFLIDKDGLITKERTNIDPAA 384
           KIVV GAGSAG+GVLN A + ++RM GN ++   +A++QF+++D  GLIT+ R NIDP A
Sbjct: 339 KIVVAGAGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEA 398

Query: 385 VPFAKDPRELE--GLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPA 444
            PFA+  +E+E  GL EGA L+EVV++V+P VLLGLS VGG+F++EVL+AM+ S S++PA
Sbjct: 399 QPFARKTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPA 458

Query: 445 IFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIG 504
           IFAMSNPT NAECT  DAF   GEN++FASGSPF+NV  GNG  GH NQ NNMYLFPGIG
Sbjct: 459 IFAMSNPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIG 518

Query: 505 LGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAV 564
           LG+LLSGA  ++DGMLQAA+ECLA+YM++EEV  GI+YP I  IR+IT  + AAV++ A+
Sbjct: 519 LGTLLSGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAI 578

Query: 565 SENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLVHE 603
            E+L EG+ ++  RE++ + +E  +EY++ NMW P Y  LV++
Sbjct: 579 EEDLVEGYREMDAREIQKLDEEGLMEYVENNMWNPEYPTLVYK 621

BLAST of PI0019452 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 7.3e-224
Identity = 377/582 (64.78%), Postives = 471/582 (80.93%), Query Frame = 0

Query: 27  IVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNT 86
           IV+KR  DIL DPWFNK T F +TERDRL +RGLLPP V++ EQQ  RF    R LE  T
Sbjct: 41  IVNKRSLDILQDPWFNKGTAFSMTERDRLDLRGLLPPNVMTTEQQIERFTADLRVLELTT 100

Query: 87  HGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLFRR 146
              P     LAKWRILNRLHDRNET++++VLI+NI+ +API+ TPTVGLVCQ +SGL+RR
Sbjct: 101 KDGPSDTYDLAKWRILNRLHDRNETMFFKVLIENIEEYAPIVSTPTVGLVCQKFSGLYRR 160

Query: 147 PRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVA 206
           PRGMYFS+ D+GEMMSM+YNWP +QVDMIV+TDGSRILGLGDLGV GIG+ IGKLD+YVA
Sbjct: 161 PRGMYFSSDDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGVAIGKLDLYVA 220

Query: 207 AAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRWPKA 266
           AAGINPQR+LPVM+D+GTNNE LL + LYLGL++ RL+GEEYL+++DEFMEAV TRWP  
Sbjct: 221 AAGINPQRVLPVMIDVGTNNEDLLKNPLYLGLQKKRLDGEEYLAVMDEFMEAVFTRWPNV 280

Query: 267 IVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQKI 326
           IVQFED Q KWA   LQRYR ++  FN D+QGT+GVA+AGLLG VRAQGRP+ DF  QKI
Sbjct: 281 IVQFEDIQNKWALTLLQRYRHKYRTFNVDVQGTSGVAIAGLLGAVRAQGRPMIDFPKQKI 340

Query: 327 VVVGAGSAGLGVLNMAIQAVSRMAGNNDST---ARNQFFLIDKDGLITKERTNIDPAAVP 386
           VV GAGS+G+GVLN A + ++RM GN++S    AR+QF+++D  GLIT++R N+DP   P
Sbjct: 341 VVAGAGSSGVGVLNAARKTMARMLGNDESAFDRARSQFWVVDDKGLITEKRANLDPEVQP 400

Query: 387 FAKDPRE--LEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPAIF 446
           FA    E  L+GL+EGA L+EVV++V+P VLLGLS  GG+F++EVL+A+++S S++PAIF
Sbjct: 401 FAWKENEISLQGLNEGAKLVEVVRQVKPDVLLGLSAYGGLFSKEVLEALKDSTSTRPAIF 460

Query: 447 AMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIGLG 506
           AMSNPT NAECT  +AF   G+++V+ASGSPF++V LGNGK GHVNQ NNMYLFPGIGLG
Sbjct: 461 AMSNPTKNAECTPEEAFSIVGDHVVYASGSPFKDVDLGNGKIGHVNQGNNMYLFPGIGLG 520

Query: 507 SLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAVSE 566
            LLSG+R I+D M QAAAE LA YMTDEEV +G++YPSI  IR+IT EV AAV++ AV E
Sbjct: 521 VLLSGSRIISDSMFQAAAERLAGYMTDEEVINGVIYPSISRIRDITKEVAAAVIKEAVEE 580

Query: 567 NLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLVHEK 604
           +LAEG+ D+  REL+ + +E+ +EYI++NMW P Y  LV++K
Sbjct: 581 DLAEGYRDMDARELQKLNEEQILEYIEKNMWNPEYPTLVYKK 622

BLAST of PI0019452 vs. ExPASy TrEMBL
Match: A0A1S3CBH2 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103499065 PE=3 SV=1)

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 598/603 (99.17%), Postives = 600/603 (99.50%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLVKFTAAS RRSR LSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLVKFTAASCRRSRWLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           I++FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG
Sbjct: 121 IRNFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDKDGLITKERTNIDPAA PFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG
Sbjct: 361 FFLIDKDGLITKERTNIDPAAAPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN
Sbjct: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. ExPASy TrEMBL
Match: A0A6J1DPR7 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111023088 PE=3 SV=1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 575/603 (95.36%), Postives = 591/603 (98.01%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL + TAAS  RSRR STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTAASLSRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPD +VSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWP QQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRL+GEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRK+FCMFNDDIQGTA
Sbjct: 241 PRLDGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKKFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMA+QAVSRMAGN +STARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAVQAVSRMAGNTNSTARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDKDGL+TK+RTNIDPAA PFAKDP ELEGLSEGA+LLEVVKKVRPHVLLGLSGVGG
Sbjct: 361 FFLIDKDGLVTKDRTNIDPAAAPFAKDPTELEGLSEGASLLEVVKKVRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDS+KPAIFAMSNPTMNAECTAADAFK+AGE+IVFASGSPFENVALGN
Sbjct: 421 IFNEEVLKAMRESDSTKPAIFAMSNPTMNAECTAADAFKHAGEHIVFASGSPFENVALGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GK GHVNQANNMYLFPGIGLG+LLSGAR+ITDGMLQAAAECLASYMTDEEVQSGILYPSI
Sbjct: 481 GKIGHVNQANNMYLFPGIGLGALLSGARFITDGMLQAAAECLASYMTDEEVQSGILYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIR+ITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMA+EETIEYIKRNMWFPIYSPLV
Sbjct: 541 DSIRQITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAEEETIEYIKRNMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. ExPASy TrEMBL
Match: A0A6J1HJ42 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111464523 PE=3 SV=1)

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 570/603 (94.53%), Postives = 587/603 (97.35%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL + T AS RRSRR STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWP QQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLD+GTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDVGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYR++FCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRRKFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDF+NQKIVVVGAGSAGLGVLNMA+QA+SRMAGN+D  ARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFINQKIVVVGAGSAGLGVLNMAVQALSRMAGNSDLNARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDK+GLITK+R NID AA PFAKDPRELEGLSEGA+LLEVVKK+RPHVLLGLSGVGG
Sbjct: 361 FFLIDKEGLITKDRKNIDSAAAPFAKDPRELEGLSEGASLLEVVKKIRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDS+KPAIFAMSNPTMNAECTAADAFKYAGEN+VFASGSPFENV  GN
Sbjct: 421 IFNEEVLKAMRESDSTKPAIFAMSNPTMNAECTAADAFKYAGENVVFASGSPFENVDFGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGAR+ITDGMLQAAAECLASYMTDEEVQ+G+LYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARFITDGMLQAAAECLASYMTDEEVQNGVLYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELR MAKEETIEYIK NMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRQMAKEETIEYIKENMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. ExPASy TrEMBL
Match: A0A6J1KE67 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111494177 PE=3 SV=1)

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 571/603 (94.69%), Postives = 586/603 (97.18%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL + T AS RRSRR STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWP QQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLD+GTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDVGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYR++FCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRRKFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDF NQKIVVVGAGSAGLGVLNMA+QA+SRMAGN+D TARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFTNQKIVVVGAGSAGLGVLNMAVQALSRMAGNSDLTARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDK+GLITK+R NID AA PFAKDPRELEGLSEGA+LLEVVKK+RPHVLLGLSGVGG
Sbjct: 361 FFLIDKEGLITKDRKNIDSAAAPFAKDPRELEGLSEGASLLEVVKKIRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDS+KPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENV  GN
Sbjct: 421 IFNEEVLKAMRESDSTKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVDFGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGAR+ITDGMLQAAAECLASYMTDEEVQ+G+LYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARFITDGMLQAAAECLASYMTDEEVQNGVLYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELR M KEETIEYIK NMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRQMEKEETIEYIKENMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. ExPASy TrEMBL
Match: A0A251LW48 (Malic enzyme OS=Manihot esculenta OX=3983 GN=MANES_01G260100 PE=3 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 543/603 (90.05%), Postives = 577/603 (95.69%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWN+ +F  +S RRSRR STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLG+RGL
Sbjct: 1   MWNVARFATSSLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGLRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLE+NT GQP+ +VSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLEKNTQGQPNSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWP QQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLD+GTNNEKLL DRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDVGTNNEKLLEDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAV TRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDD+QGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVFTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDVQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAG N++  +NQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGKNEAAVKNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFL+DKDGLITKERTNIDPAA PFAKD +++EGL EGA+L+EVVKK++PHVLLGLSGVGG
Sbjct: 361 FFLLDKDGLITKERTNIDPAAAPFAKDLKDVEGLREGASLIEVVKKLKPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRES+S+KPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENV LGN
Sbjct: 421 IFNEEVLKAMRESESTKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVDLGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GK GHVNQANNMYLFPGIGLG+LLSGAR ITD MLQAA+ECLASYMTDEE+Q GILYPS+
Sbjct: 481 GKVGHVNQANNMYLFPGIGLGTLLSGARIITDEMLQAASECLASYMTDEEIQKGILYPSV 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           ++IR ITAEVGAAVLR+AV+E LAEG+GDVGPRELR M+KEET+EYI RNMWFP+YSPLV
Sbjct: 541 NNIRHITAEVGAAVLRAAVAEGLAEGYGDVGPRELRHMSKEETVEYITRNMWFPVYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. NCBI nr
Match: XP_008460179.1 (PREDICTED: NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 598/603 (99.17%), Postives = 600/603 (99.50%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLVKFTAAS RRSR LSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLVKFTAASCRRSRWLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           I++FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG
Sbjct: 121 IRNFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDKDGLITKERTNIDPAA PFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG
Sbjct: 361 FFLIDKDGLITKERTNIDPAAAPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN
Sbjct: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. NCBI nr
Match: XP_038878987.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Benincasa hispida] >XP_038878988.1 NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Benincasa hispida] >XP_038878989.1 NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Benincasa hispida])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 590/603 (97.84%), Postives = 596/603 (98.84%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL KFTAAS  RSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLAKFTAASLSRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLD+GTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDVGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPL DF+NQKIVVVGAGSAGLGVLNMA+QAVSRMAGN+DS ARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLRDFINQKIVVVGAGSAGLGVLNMAVQAVSRMAGNSDSMARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDKDGLITKERTNIDPAA PFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG
Sbjct: 361 FFLIDKDGLITKERTNIDPAAAPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN
Sbjct: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGAR+ITDGMLQAAAECLASYMTDEEVQSGILYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARFITDGMLQAAAECLASYMTDEEVQSGILYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYI+RNMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIQRNMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. NCBI nr
Match: XP_031743262.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucumis sativus] >KAE8646688.1 hypothetical protein Csa_005626 [Cucumis sativus])

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 594/603 (98.51%), Postives = 597/603 (99.00%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLVKFTAASFRRSRRLSTAIPGP IVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLVKFTAASFRRSRRLSTAIPGPSIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQP G VSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPYGTVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK+FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG
Sbjct: 121 IKNFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLND LYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDPLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTAR+Q
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARSQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDKDGLITKERTNIDPAA PFAKDPRELEGLSEGANLLEVVK+VRPHVLLGLSGVGG
Sbjct: 361 FFLIDKDGLITKERTNIDPAAAPFAKDPRELEGLSEGANLLEVVKRVRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN
Sbjct: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPREL LMAKEETIEYIKRNMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELGLMAKEETIEYIKRNMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. NCBI nr
Match: XP_022156123.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Momordica charantia])

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 575/603 (95.36%), Postives = 591/603 (98.01%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL + TAAS  RSRR STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTAASLSRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPD +VSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWP QQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRL+GEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRK+FCMFNDDIQGTA
Sbjct: 241 PRLDGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKKFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMA+QAVSRMAGN +STARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAVQAVSRMAGNTNSTARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDKDGL+TK+RTNIDPAA PFAKDP ELEGLSEGA+LLEVVKKVRPHVLLGLSGVGG
Sbjct: 361 FFLIDKDGLVTKDRTNIDPAAAPFAKDPTELEGLSEGASLLEVVKKVRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDS+KPAIFAMSNPTMNAECTAADAFK+AGE+IVFASGSPFENVALGN
Sbjct: 421 IFNEEVLKAMRESDSTKPAIFAMSNPTMNAECTAADAFKHAGEHIVFASGSPFENVALGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GK GHVNQANNMYLFPGIGLG+LLSGAR+ITDGMLQAAAECLASYMTDEEVQSGILYPSI
Sbjct: 481 GKIGHVNQANNMYLFPGIGLGALLSGARFITDGMLQAAAECLASYMTDEEVQSGILYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIR+ITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMA+EETIEYIKRNMWFPIYSPLV
Sbjct: 541 DSIRQITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAEEETIEYIKRNMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. NCBI nr
Match: XP_023514814.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 572/603 (94.86%), Postives = 588/603 (97.51%), Query Frame = 0

Query: 1   MWNLVKFTAASFRRSRRLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL + T AS RRSRR STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 IKSFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDG 180
           IK FAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWP QQVDMIVLTDG
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDG 180

Query: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQ 240
           SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLD+GTNNEKLLNDRLYLGLRQ
Sbjct: 181 SRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDVGTNNEKLLNDRLYLGLRQ 240

Query: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTA 300
           PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYR++FCMFNDDIQGTA
Sbjct: 241 PRLEGEEYLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRRKFCMFNDDIQGTA 300

Query: 301 GVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQ 360
           GVALAGLLGTVRAQGRPLSDF+NQKIVVVGAGSAGLGVLNMA+QA+SRMAGN+D TARNQ
Sbjct: 301 GVALAGLLGTVRAQGRPLSDFINQKIVVVGAGSAGLGVLNMAVQALSRMAGNSDLTARNQ 360

Query: 361 FFLIDKDGLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGG 420
           FFLIDK+GLITK+R NID AA PFAKDPRELEGLSEGA+LLEVVKK+RPHVLLGLSGVGG
Sbjct: 361 FFLIDKEGLITKDRKNIDSAAAPFAKDPRELEGLSEGASLLEVVKKIRPHVLLGLSGVGG 420

Query: 421 IFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGN 480
           IFNEEVLKAMRESDS+KPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENV  GN
Sbjct: 421 IFNEEVLKAMRESDSTKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVDFGN 480

Query: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSI 540
           GKFGHVNQANNMYLFPGIGLGSLLSGAR+ITDGMLQAAAECLASYMTDEEVQ+G+LYPSI
Sbjct: 481 GKFGHVNQANNMYLFPGIGLGSLLSGARFITDGMLQAAAECLASYMTDEEVQNGVLYPSI 540

Query: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLV 600
           DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELR MAKEETIEYIK NMWFPIYSPLV
Sbjct: 541 DSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELRQMAKEETIEYIKENMWFPIYSPLV 600

Query: 601 HEK 604
           HEK
Sbjct: 601 HEK 603

BLAST of PI0019452 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 1011.9 bits (2615), Expect = 2.1e-295
Identity = 493/596 (82.72%), Postives = 545/596 (91.44%), Query Frame = 0

Query: 9   AASFRRSRR-LSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVIS 68
           AA    SRR  STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRV++
Sbjct: 15  AARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMT 74

Query: 69  FEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPI 128
             QQ  RF+ES+RSLE NT G+P+ +V+LAKWR+LNRLHDRNETLYYRVLIDNIK FAPI
Sbjct: 75  CVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYYRVLIDNIKDFAPI 134

Query: 129 IYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLG 188
           IYTPTVGLVCQNYSGL+RRPRGMYFSAKDKGEMMSMIYNWP  QVDMIV+TDGSRILGLG
Sbjct: 135 IYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDMIVITDGSRILGLG 194

Query: 189 DLGVQGIGIPIGKLDMYVAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEE 248
           DLGVQGIGIPIGKLDMYVAAAGINPQR+LP+MLD+GTNNEKLL + LYLG+RQPRLEGEE
Sbjct: 195 DLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDLYLGVRQPRLEGEE 254

Query: 249 YLSIVDEFMEAVHTRWPKAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGL 308
           YL I+DEFMEA  TRWPKA+VQFEDFQ KWAF TL+RYRK+FCMFNDD+QGTAGVALAGL
Sbjct: 255 YLEIIDEFMEAAFTRWPKAVVQFEDFQAKWAFGTLERYRKKFCMFNDDVQGTAGVALAGL 314

Query: 309 LGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAGNNDSTARNQFFLIDKD 368
           LGTVRAQGRP+SDFVNQKIVVVGAGSAGLGV  MA+QAV+RMAG ++S A   F+LIDKD
Sbjct: 315 LGTVRAQGRPISDFVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISESEATKNFYLIDKD 374

Query: 369 GLITKERTNIDPAAVPFAKDPRELEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVL 428
           GL+T ERT +DP AV FAK+P E+    EGA+++EVVKKVRPHVLLGLSGVGGIFNEEVL
Sbjct: 375 GLVTTERTKLDPGAVLFAKNPAEIR---EGASIVEVVKKVRPHVLLGLSGVGGIFNEEVL 434

Query: 429 KAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVN 488
           KAMRESDS KPAIFAMSNPT+NAECTAADAFK+AG NIVFASGSPFENV L NGK GHVN
Sbjct: 435 KAMRESDSCKPAIFAMSNPTLNAECTAADAFKHAGGNIVFASGSPFENVELENGKVGHVN 494

Query: 489 QANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREIT 548
           QANNMYLFPGIGLG+LLSGAR +TDGMLQAA+ECLASYMTDEEVQ GILYPSI++IR IT
Sbjct: 495 QANNMYLFPGIGLGTLLSGARIVTDGMLQAASECLASYMTDEEVQKGILYPSINNIRHIT 554

Query: 549 AEVGAAVLRSAVSENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLVHEK 604
           AEVGAAVLR+AV++++AEGHGDVGP++L  M+KE+T+ YI RNMWFP+YSPLVHEK
Sbjct: 555 AEVGAAVLRAAVTDDIAEGHGDVGPKDLSHMSKEDTVNYITRNMWFPVYSPLVHEK 607

BLAST of PI0019452 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 802.4 bits (2071), Expect = 2.6e-232
Identity = 383/583 (65.69%), Postives = 475/583 (81.48%), Query Frame = 0

Query: 25  PCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLER 84
           P IVHK+G DILHDPWFNK T F +TER+RL +RGLLPP V+  EQQ  RFM   + LE 
Sbjct: 39  PTIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEE 98

Query: 85  NTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLF 144
                P    +LAKWRILNRLHDRNET+YY+VLI+NI+ +API+YTPTVGLVCQNYSGLF
Sbjct: 99  QARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLF 158

Query: 145 RRPRGMYFSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMY 204
           RRPRGMYFSA+D+GEMMSM+YNWP +QVDMIV+TDGSRILGLGDLGV GIGI +GKLD+Y
Sbjct: 159 RRPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLY 218

Query: 205 VAAAGINPQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRWP 264
           VAAAGINPQR+LPVM+D+GTNNEKL ND +YLGL+Q RLE ++Y+ ++DEFMEAV+TRWP
Sbjct: 219 VAAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWP 278

Query: 265 KAIVQFEDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQ 324
             IVQFEDFQ KWAF+ LQRYR  + MFNDD+QGTAGVA+AGLLG VRAQGRP+ DF   
Sbjct: 279 HVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKM 338

Query: 325 KIVVVGAGSAGLGVLNMAIQAVSRMAGNNDS---TARNQFFLIDKDGLITKERTNIDPAA 384
           KIVV GAGSAG+GVLN A + ++RM GN ++   +A++QF+++D  GLIT+ R NIDP A
Sbjct: 339 KIVVAGAGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEA 398

Query: 385 VPFAKDPRELE--GLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPA 444
            PFA+  +E+E  GL EGA L+EVV++V+P VLLGLS VGG+F++EVL+AM+ S S++PA
Sbjct: 399 QPFARKTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPA 458

Query: 445 IFAMSNPTMNAECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIG 504
           IFAMSNPT NAECT  DAF   GEN++FASGSPF+NV  GNG  GH NQ NNMYLFPGIG
Sbjct: 459 IFAMSNPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIG 518

Query: 505 LGSLLSGARYITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAV 564
           LG+LLSGA  ++DGMLQAA+ECLA+YM++EEV  GI+YP I  IR+IT  + AAV++ A+
Sbjct: 519 LGTLLSGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAI 578

Query: 565 SENLAEGHGDVGPRELRLMAKEETIEYIKRNMWFPIYSPLVHE 603
            E+L EG+ ++  RE++ + +E  +EY++ NMW P Y  LV++
Sbjct: 579 EEDLVEGYREMDAREIQKLDEEGLMEYVENNMWNPEYPTLVYK 621

BLAST of PI0019452 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-122
Identity = 238/561 (42.42%), Postives = 337/561 (60.07%), Query Frame = 0

Query: 32  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPD 91
           G  ++ DP +NK   F   ERD   I GLLPP V+S + Q  + M + R           
Sbjct: 49  GYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQY--------- 108

Query: 92  GIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLFRRPRGMY 151
             V L ++  L  L +RNE L+Y++LIDN++   P++YTPTVG  CQ Y  ++RRP+G+Y
Sbjct: 109 -TVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLY 168

Query: 152 FSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGIN 211
            S K+KG+++ ++ NWP + + +IV+TDG RILGLGDLG QG+GIP+GKL +Y A  GI 
Sbjct: 169 ISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIR 228

Query: 212 PQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRW-PKAIVQF 271
           P   LP+ +D+GTNNEKLLN+  Y+GL+Q R  GEEY   + EFM AV   +  K +VQF
Sbjct: 229 PSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYGEKVLVQF 288

Query: 272 EDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQKIVVVG 331
           EDF    AFE L +Y     +FNDDIQGTA V LAGL+   +  G+ L+D      + +G
Sbjct: 289 EDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLAD---HTFLFLG 348

Query: 332 AGSAGLGVLNMAIQAVSRMAGNNDSTARNQFFLIDKDGLITKER-TNIDPAAVPFAKDPR 391
           AG AG G+  +    +S+  G      R + +L+D  GLI  ER  ++     P+A D +
Sbjct: 349 AGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHK 408

Query: 392 ELEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPAIFAMSNPTMN 451
            ++       LL  V  ++P VL+G SGVG  F +EV++AM  + + KP I A+SNPT  
Sbjct: 409 PVK------ELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAM-ATLNEKPLILALSNPTSQ 468

Query: 452 AECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIGLGSLLSGARY 511
           AECTA +A+ +     +FASGSPF+ V     KF    QANN Y+FPG+GLG ++SGA  
Sbjct: 469 AECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKF-TPGQANNCYIFPGLGLGLIMSGAIR 528

Query: 512 ITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAVSENLAEGHGD 571
           + D ML AA+E LAS +T+E   +G++YP   +IR+I+A + A+V   A +  L      
Sbjct: 529 VRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASV--GAKTYELGLASNL 586

Query: 572 VGPRELRLMAKEETIEYIKRN 591
             P++L  MA+      + RN
Sbjct: 589 PRPKDLVKMAESCMYSPVYRN 586

BLAST of PI0019452 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 434.5 bits (1116), Expect = 1.4e-121
Identity = 235/567 (41.45%), Postives = 340/567 (59.96%), Query Frame = 0

Query: 32  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPD 91
           G  +L DP +NK   F   ERD   +RGLLPP V+  + Q  R + + R  +        
Sbjct: 42  GYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQ-------- 101

Query: 92  GIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLFRRPRGMY 151
               L K+  L  L +RNE L+Y++LIDN++   PI+YTPTVG  CQ +  +FRRP+G++
Sbjct: 102 --FPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLF 161

Query: 152 FSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGIN 211
            S KDKG+++ ++ NWP + + +IV+TDG RILGLGDLG QG+GIP+GKL +Y A  G+ 
Sbjct: 162 ISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVR 221

Query: 212 PQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRW-PKAIVQF 271
           P   LPV +D+GTNNEKLLND  Y+GLRQ R  G+EY  +++EFM AV   +  K ++QF
Sbjct: 222 PSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELLNEFMSAVKQNYGEKVLIQF 281

Query: 272 EDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQKIVVVG 331
           EDF    AFE L +Y     +FNDDIQGTA V LAGL+   +    PL++      + +G
Sbjct: 282 EDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQKLTNSPLAE---HTFLFLG 341

Query: 332 AGSAGLGVLNMAIQAVSRMAGNNDSTARNQFFLIDKDGLITKER-TNIDPAAVPFAKDPR 391
           AG AG G+  +    +S+    +   +R + +L+D  GLI   R  ++     P+A +  
Sbjct: 342 AGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSRKDSLQDFKKPWAHEHE 401

Query: 392 ELEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPAIFAMSNPTMN 451
            ++      +LL  +K ++P VL+G SGVG  F +EV++AM  S + +P I A+SNPT  
Sbjct: 402 PVK------DLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAM-SSINERPLIMALSNPTTQ 461

Query: 452 AECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIGLGSLLSGARY 511
           +ECTA +A+ ++    +FASGSPF+ V    GK     QANN Y+FPG GLG ++SGA  
Sbjct: 462 SECTAEEAYTWSKGRAIFASGSPFDPVEY-EGKVFVSTQANNAYIFPGFGLGLVISGAIR 521

Query: 512 ITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAVSENLAEGHGD 571
           + D ML AAAE LA  ++ E  + G++YPS  SIR+I+A++ A V   A    LA     
Sbjct: 522 VHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYELGLAG---- 577

Query: 572 VGPRELRLMAKEETIEYIKRNMWFPIY 597
                 RL   ++ ++  + +M+ P Y
Sbjct: 582 ------RLPRPKDIVKCAESSMYSPTY 577

BLAST of PI0019452 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 429.5 bits (1103), Expect = 4.5e-120
Identity = 228/567 (40.21%), Postives = 338/567 (59.61%), Query Frame = 0

Query: 32  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPD 91
           G  ++ DP +NK   F   ERD   + GLLPP ++S + Q  + M + R           
Sbjct: 49  GYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQY--------- 108

Query: 92  GIVSLAKWRILNRLHDRNETLYYRVLIDNIKSFAPIIYTPTVGLVCQNYSGLFRRPRGMY 151
             V L ++  L  L +RNE L+Y++LIDN++   P++YTPTVG  CQ Y  +FR+P+G+Y
Sbjct: 109 -TVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLY 168

Query: 152 FSAKDKGEMMSMIYNWPGQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGIN 211
            S  +KG+++ ++ NWP + + +IV+TDG RILGLGDLG QG+GIP+GKL +Y A  GI 
Sbjct: 169 ISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIR 228

Query: 212 PQRILPVMLDIGTNNEKLLNDRLYLGLRQPRLEGEEYLSIVDEFMEAVHTRW-PKAIVQF 271
           P   LP+ +D+GTNNEKLLND  Y+GL+Q R  G+EY   + EFM AV   +  K +VQF
Sbjct: 229 PSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYGEKVLVQF 288

Query: 272 EDFQMKWAFETLQRYRKRFCMFNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQKIVVVG 331
           EDF    AF+ L +Y     +FNDDIQGTA V LAGL+   +  G+ L+D      + +G
Sbjct: 289 EDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLAD---HTFLFLG 348

Query: 332 AGSAGLGVLNMAIQAVSRMAGNNDSTARNQFFLIDKDGLITKER-TNIDPAAVPFAKDPR 391
           AG AG G+  +    +S+  G   +  R + +L+D  GLI   R  ++     P+A + +
Sbjct: 349 AGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHK 408

Query: 392 ELEGLSEGANLLEVVKKVRPHVLLGLSGVGGIFNEEVLKAMRESDSSKPAIFAMSNPTMN 451
            ++      +L+  V  ++P VL+G SGVG  F +EV++AM  +++ KP I A+SNPT  
Sbjct: 409 PVK------DLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAM-ATNNEKPLILALSNPTSQ 468

Query: 452 AECTAADAFKYAGENIVFASGSPFENVALGNGKFGHVNQANNMYLFPGIGLGSLLSGARY 511
           AECTA  A+ +     +F SGSPF+ V   +GK     QANN Y+FPG+GLG ++SGA  
Sbjct: 469 AECTAEQAYTWTKGRAIFGSGSPFDPVVY-DGKTYLPGQANNCYIFPGLGLGLIMSGAIR 528

Query: 512 ITDGMLQAAAECLASYMTDEEVQSGILYPSIDSIREITAEVGAAVLRSAVSENLAEGHGD 571
           + D ML AA+E LA+ +T+E   +G++YP   +IREI+A + A V        LA     
Sbjct: 529 VRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLAS---- 584

Query: 572 VGPRELRLMAKEETIEYIKRNMWFPIY 597
                  L   ++ +++ + +M+ P+Y
Sbjct: 589 ------NLPRAKDLVKFAESSMYSPVY 584

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P372259.9e-29882.78NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX... [more]
Q8L7K93.0e-29482.72NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N... [more]
P372214.5e-23465.95NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX... [more]
Q9SIU03.6e-23165.69NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N... [more]
P372247.3e-22464.78NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond... [more]
Match NameE-valueIdentityDescription
A0A1S3CBH20.0e+0099.17Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103499065 PE=3 SV=1[more]
A0A6J1DPR70.0e+0095.36Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111023088 PE=3 SV=1[more]
A0A6J1HJ420.0e+0094.53Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111464523 PE=3 SV=1[more]
A0A6J1KE670.0e+0094.69Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111494177 PE=3 SV=1[more]
A0A251LW480.0e+0090.05Malic enzyme OS=Manihot esculenta OX=3983 GN=MANES_01G260100 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008460179.10.0e+0099.17PREDICTED: NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [... [more]
XP_038878987.10.0e+0097.84NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Benincasa hispida] >XP... [more]
XP_031743262.10.0e+0098.51NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucumis sat... [more]
XP_022156123.10.0e+0095.36NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Momordica charantia][more]
XP_023514814.10.0e+0094.86NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucurbita p... [more]
Match NameE-valueIdentityDescription
AT4G00570.12.1e-29582.72NAD-dependent malic enzyme 2 [more]
AT2G13560.12.6e-23265.69NAD-dependent malic enzyme 1 [more]
AT5G25880.16.3e-12242.42NADP-malic enzyme 3 [more]
AT2G19900.11.4e-12141.45NADP-malic enzyme 1 [more]
AT5G11670.14.5e-12040.21NADP-malic enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 208..230
score: 59.78
coord: 292..308
score: 79.12
coord: 112..136
score: 52.1
coord: 267..285
score: 50.66
coord: 435..451
score: 54.26
coord: 172..201
score: 71.08
coord: 326..342
score: 44.71
IPR001891Malic oxidoreductasePIRSFPIRSF000106MEcoord: 6..602
e-value: 1.4E-212
score: 705.3
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 106..286
e-value: 3.5E-94
score: 328.9
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 106..286
e-value: 3.3E-75
score: 251.9
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 296..559
e-value: 8.2E-90
score: 314.4
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 296..558
e-value: 2.7E-93
score: 312.0
IPR037062Malic enzyme, N-terminal domain superfamilyGENE3D3.40.50.10380coord: 30..293
e-value: 5.7E-126
score: 420.8
NoneNo IPR availableGENE3D3.40.50.720coord: 294..603
e-value: 9.2E-102
score: 342.4
NoneNo IPR availablePIRSRPIRSR000106-3PIRSR000106-3coord: 34..375
e-value: 3.9E-130
score: 432.7
NoneNo IPR availablePIRSRPIRSR000106-1PIRSR000106-1coord: 35..373
e-value: 1.8E-120
score: 400.9
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 1..599
NoneNo IPR availablePANTHERPTHR23406:SF73NAD-DEPENDENT MALIC ENZYME 2, MITOCHONDRIALcoord: 1..599
NoneNo IPR availableCDDcd05312NAD_bind_1_malic_enzcoord: 296..594
e-value: 1.70053E-127
score: 374.193
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 18..295
IPR015884Malic enzyme, conserved sitePROSITEPS00331MALIC_ENZYMEScoord: 292..308
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 296..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0019452.1PI0019452.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
biological_process GO:0006090 pyruvate metabolic process
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004470 malic enzyme activity