PI0019284 (gene) Melon (PI 482460) v1

Overview


Name	PI0019284
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	DNA-directed RNA polymerase II subunit 4
Location	chr03: 24165770 .. 24167961 (+)
RNA-Seq Expression	PI0019284
Synteny	PI0019284

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGGGGAGGAGGAAGAAAATGCTGCTGAGCTTAAAATTGGAGATGGTAGAAATCTATCTATAAATTAAAACTTCCCATCTATATTCAATGATTTTAAGTTTCCCATACTTTTATGGTGATGGGTTTCCTTCATATATTTGTAAAATTAGAAGTTTCTAAGCAACTGATGTATTTAATTTTTTATTTTGCTCAAAAAGTTAGAAAATTTATTATGTTATACCCCATGACTTATTATATGTATCATTATTACGAAACCTTTGATCCTTCTAGTAGCCAATACCAATAGGGAGACTGTTCTTATGTACAATGCATATAAATATAGTTTAGATTGGAAATGTGGAGTTAATGCACTAGTTTATCTTCAAGCAGTTTTATGGGTAGTGTGGTAATAATCTCTTCGGCCTGAGAGGAAAAAGACTGAACTTCCTTAGTTCTTTAAGATCGATAGTTATGTAATTATATCTTGTAGCCATCTAGTATCTCATAACTTTCTCAGATATTGTTCGGTAAGTCATAGTCATTTGATTGGTGACTTGACACGTCAACAAAGAAGTTTCTTTTGTATATACCTTCCCATTGTGTTTTATGATATCATGTTATTGTTTTTCATAATGCATTCTCAATATTTTAAGCTAGCTCATTTACTACATCAAAGGAATACTTCCTTCACATGATATTTAAGGGTCGAATGGCTGACCGCTTTGAGTTTTCTTATTTTTGGATTAATATCATATTGATTTTTAGGTTTTTTAAAATTTTAGATGAAAATGTGTGATCGATGCAGTATCTAAATTCTTATCTATGATTATTCTGGCAAAAAGATATTCAAGTATTGTAAGTTTTTCAGTTCTTTATGAATCTATGATAATGCGGACTCAAGAATGACCAAATATTTGGTTTACATAAGTAATTGTGCAAAATTCAGGCTCTTGCACCCAAATAATTTTGGTGTACATTCTAGGGTTTGTAAGGTAATAACTGGCTTTTACTTTCTTTGGTTACTTGCAGAGTTCTTAAAAGCCAAATGTTTAATGAATTGTGAAGTTTCTCTAATCCTTGAACATAAATATGAGCAACTTCAACAGATGTCTGAGGATCCAACAAATCAAGTTTCCCAGTGAGTTTTATTATGTATATCCTTTATTGATTCATGAGTTAATTTAGAGTCTCCGTAGTTGCTTAGAAAGCTAAAATATATTGCTAAGTTACTATATCATTTTCTAAAAAATAAAACGACTAGAAAATTCTTAATTATGGTCTTATCTTGCAGAGTGTTTGAAAAGTCACTTCAATATGTGAAACGCTTCAGCCGTTATAAGAATCCTGATGCTGTTAGACAAGTTCGAGAGTATCCTTAAAACTATATATGTTGATGTTTTTCATTTTATGGGATAGTGGGTGGGAAGGCATGTTTCATTTTATGTGATGGTGGGAATGGTCAACTGCCAAGCCAAAAACTCTCTTTGGTTAATTTTTTTTTGGGAAATACTTAATAAATTAGTTCATTATAAGCATACTATTTACCAACGTAATTATGAATTGAAAGTTTGGGTATCATTGTTTAGAAGTGGATTAAATAATGTGTAATTGTTATCTTTTGAGATATTGAATCTTAACAAACAAACAGAGTTCTAAGCAGGTACCAGTTGGCGGAGTTTGAGGTATGTACACTAGACACCTTGGTTTAATCATTTTGTGTAATATTATTATCTCTTTCTTTTTTGAGGAAACGCTCATCTCATTAGTTTTGAAATTTGCAGCTTTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAAGAAGCCATTGCCATGGTACCATCATTGAAGGTATCTTTTGCCACTTTTGTGCTATTGGATTAGCAAGTATCAATCGTTTTTCTGTTGCTTGAAACCTATAGTATATTTATAGGTATTTTGCTAAATCATAGATAGGTACAATTTTTTGGAAGTTTACTATTGTTTTTATCTCTCAACATTGAACATGCGTCAAATTTTAAGATGTGTTACTTACAACTTTTAAATTTTGATATAATCTCTTTTTCTTTTCCATTTTTTGAAAACAGAAGTTAATCGCTTGTGAATGATTTTCTTTTTGTTGGTTTCAGACCAAGGGAAGGGTGCATGACGATGAAGCAATTGAGAAGATGTTGAATGATCTTTCATTAATAAAAGATTTGAGTAGCTAG

mRNA sequence

ATGTCCGGGGAGGAGGAAGAAAATGCTGCTGAGCTTAAAATTGGAGATGAGTTCTTAAAAGCCAAATGTTTAATGAATTGTGAAGTTTCTCTAATCCTTGAACATAAATATGAGCAACTTCAACAGATGTCTGAGGATCCAACAAATCAAGTTTCCCAAGTGTTTGAAAAGTCACTTCAATATGTGAAACGCTTCAGCCGTTATAAGAATCCTGATGCTGTTAGACAAGTTCGAGAAGTTCTAAGCAGGTACCAGTTGGCGGAGTTTGAGCTTTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAAGAAGCCATTGCCATGGTACCATCATTGAAGACCAAGGGAAGGGTGCATGACGATGAAGCAATTGAGAAGATGTTGAATGATCTTTCATTAATAAAAGATTTGAGTAGCTAG

Coding sequence (CDS)

ATGTCCGGGGAGGAGGAAGAAAATGCTGCTGAGCTTAAAATTGGAGATGAGTTCTTAAAAGCCAAATGTTTAATGAATTGTGAAGTTTCTCTAATCCTTGAACATAAATATGAGCAACTTCAACAGATGTCTGAGGATCCAACAAATCAAGTTTCCCAAGTGTTTGAAAAGTCACTTCAATATGTGAAACGCTTCAGCCGTTATAAGAATCCTGATGCTGTTAGACAAGTTCGAGAAGTTCTAAGCAGGTACCAGTTGGCGGAGTTTGAGCTTTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAAGAAGCCATTGCCATGGTACCATCATTGAAGACCAAGGGAAGGGTGCATGACGATGAAGCAATTGAGAAGATGTTGAATGATCTTTCATTAATAAAAGATTTGAGTAGCTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDDEAIEKMLNDLSLIKDLSS

Homology

[Close]

BLAST of PI0019284 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 1.9e-66
Identity = 126/135 (93.33%), Postives = 132/135 (97.78%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSR+QL EFELCVLGNLCPETVEEA+AMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSL+K
Sbjct: 121 DEAIEKMLNDLSLVK 135

[Close]

BLAST of PI0019284 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-20
Identity = 55/130 (42.31%), Postives = 90/130 (69.23%), Query Frame = 0


Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDL 132
           E ++++L+D+
Sbjct: 130 E-LQQILDDI 134

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BLAST of PI0019284 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-20
Identity = 55/130 (42.31%), Postives = 90/130 (69.23%), Query Frame = 0


Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDL 132
           E ++++L+D+
Sbjct: 130 E-LQQILDDI 134

[Close]

BLAST of PI0019284 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.1e-17
Identity = 54/145 (37.24%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHK----------YEQ---LQQMSEDPTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+          + Q   L  +  +  N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA +++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKTKGRVHDDEAIEKMLNDLSLIK 136
           SLK   ++ DDE ++ +L++LS ++
Sbjct: 132 SLK---KMEDDE-LQAILDELSNLR 152

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BLAST of PI0019284 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-16
Identity = 50/132 (37.88%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  S D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDDEAIE 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA A++PSL  +GR  D+E + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGRFEDEE-LR 130

Query: 126 KMLNDLSLIKDL 138
           ++L+D+   + L
Sbjct: 131 QILDDIGTKRSL 137

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BLAST of PI0019284 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.3e-67
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. ExPASy TrEMBL
Match: A0A0A0K7V5 (RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.3e-67
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. ExPASy TrEMBL
Match: A0A1S3C4R9 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo OX=3656 GN=LOC103496636 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.3e-67
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.9e-66
Identity = 132/135 (97.78%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.9e-66
Identity = 132/135 (97.78%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. NCBI nr
Match: XP_004138390.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDICTED: DNA-directed RNA polymerase II subunit 4 [Cucumis melo] >XP_011656444.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAA0051957.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa] >KGN45845.1 hypothetical protein Csa_005663 [Cucumis sativus] >TYK04594.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 264.6 bits (675), Expect = 4.8e-67
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. NCBI nr
Match: XP_038884885.1 (DNA-directed RNA polymerase II subunit 4 [Benincasa hispida])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. NCBI nr
Match: XP_022939401.1 (DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022993195.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_022993196.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_023549816.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >XP_023549818.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >KAG6578503.1 DNA-directed RNA polymerase II subunit 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 261.5 bits (667), Expect = 4.0e-66
Identity = 132/135 (97.78%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. NCBI nr
Match: XP_022133507.1 (DNA-directed RNA polymerase II subunit 4 [Momordica charantia])

HSP 1 Score: 260.4 bits (664), Expect = 9.0e-66
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. NCBI nr
Match: XP_022954766.1 (DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954773.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954778.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022990067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_022990068.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_022990070.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_023536652.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.0 bits (663), Expect = 1.2e-65
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKAKGRVHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSLIK
Sbjct: 121 DEAIEKMLNDLSLIK 135

[Close]

BLAST of PI0019284 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 253.1 bits (645), Expect = 1.3e-67
Identity = 126/135 (93.33%), Postives = 132/135 (97.78%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSR+QL EFELCVLGNLCPETVEEA+AMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIK 136
           DEAIEKMLNDLSL+K
Sbjct: 121 DEAIEKMLNDLSLVK 135

[Close]

BLAST of PI0019284 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-16
Identity = 44/115 (38.26%), Postives = 74/115 (64.35%), Query Frame = 0


Query: 21  AKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREV 80
           A+C+M+CE   IL+    QL  +SEDP+ ++   ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDDEAIEKMLNDLSLIK 136
           L  Y +++ E+CV+ N   E+V+E +A +PSLKTK  V  ++ ++  L +LS +K
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTKKEV-INQPLQDALEELSKLK 202

The following BLAST results are available for this feature:

BLAST of PI0019284 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48890	1.9e-66	93.33	DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]
O15514	1.6e-20	42.31	DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE...	[more]
Q9D7M8	1.6e-20	42.31	DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P...	[more]
Q54S04	2.1e-17	37.24	DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689...	[more]
Q9VEA5	1.0e-16	37.88	DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G...	[more]

BLAST of PI0019284 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C019	2.3e-67	100.00	DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0K7V5	2.3e-67	100.00	RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4...	[more]
A0A1S3C4R9	2.3e-67	100.00	DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo OX=3656 GN=LOC103496636...	[more]
A0A6J1JVN3	1.9e-66	97.78	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1FFS7	1.9e-66	97.78	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of PI0019284 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138390.1	4.8e-67	100.00	DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDI...	[more]
XP_038884885.1	6.2e-67	99.26	DNA-directed RNA polymerase II subunit 4 [Benincasa hispida]	[more]
XP_022939401.1	4.0e-66	97.78	DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402...	[more]
XP_022133507.1	9.0e-66	97.78	DNA-directed RNA polymerase II subunit 4 [Momordica charantia]	[more]
XP_022954766.1	1.2e-65	97.78	DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954773.1 DN...	[more]

BLAST of PI0019284 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09920.1	1.3e-67	93.33	RNA polymerase II, Rpb4, core protein	[more]
AT4G15950.1	1.1e-16	38.26	RNA polymerase II, Rpb4, core protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006590	RNA polymerase Rpb4/RPC9, core	SMART	SM00657	rpol4neu2	coord: 16..138 e-value: 9.2E-57 score: 204.6
IPR038324	Rpb4/RPC9 superfamily	GENE3D	1.20.1250.40		coord: 2..138 e-value: 7.7E-51 score: 173.4
IPR005574	RNA polymerase subunit RPB4/RPC9	PFAM	PF03874	RNA_pol_Rpb4	coord: 23..132 e-value: 1.5E-23 score: 83.5
None	No IPR available	PANTHER	PTHR21297:SF3	DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKE	coord: 1..136
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 1..136
IPR010997	HRDC-like superfamily	SUPERFAMILY	47819	HRDC-like	coord: 3..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019284.1	PI0019284.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031990	mRNA export from nucleus in response to heat stress
biological_process	GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0034402	recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0000932	P-body
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0030880	RNA polymerase complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0031369	translation initiation factor binding