PI0019216 (gene) Melon (PI 482460) v1

Overview
NamePI0019216
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionVicilin-like seed storage protein
Locationchr11: 5945873 .. 5948211 (-)
RNA-Seq ExpressionPI0019216
SyntenyPI0019216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAACAGAAGCAAAGATGGGGAAGAAAGAAGCCCTTTTGATACTGCTGATTGTTGCGGTTTTGGGAAATGCAATTGGGATTAAGGAGGAAGAAGAAGAAGAATGGTGGAGAAAGAGAGAAGAAGAGAAAGAGTTTGGAAGGAAAGAAAGGTTTCTAATGGAGGACTCGAAGAAGGTGATAGAGACAGAAGCAGGAGAAATGAGGGTTATGAGAAGTCCAGCTTCAAGAATTCTTGACAGGCCCATGCATATTGGCTTCATCACCATGGAACCTAAATCCTTGTTTGTCCCTCAGTATTTGGACTCCACCTTGATTCTCTTCGTCCGTAGAGGTAATTCACTGTTCTTTCTCAACTCTTTCTTGCTTACATGCATCCATATATCACTATTACAACTCCAAAAGCACACTAGCATAGTGAACCAATAAGTGATTTTCAATATTAATGGAAGGAAATGACATGAAGAGCAGGCTAGGATGGGATTTTGAATTGCTTAAAATCACTCATATCGTGTTCAAAATTCTGAAATAACCATATCTTGAATGATTTTGAACATGACAAAAGTGATATTGTGATTTTATCCCCAAATAGGATCAATACAGGACCCATGTTAATATTAGTTCATATTGTTAACATGCAATTAGTTTACGGAAGCTAGAATGTGAACGAAATGGGAGACAATTGAAAGGAATGGGGAATGTTTAAACGATTGCAGGGGATGTGAAAGTGGGATTGATTTACAAAGATGAGTTAGCAGAGAGGAGGATGAAGGGTGGGGACGTGTTCCGAATCCCAGCCGGTTCAGTATTTTACATGGTGAATGTAGGGGAAGGACAGAGACTTCAGATAATCTGCAGCATTGATAAATCTGAGAGTTTGAGTTATGGTACTTTCCAGGTAAATTATCCAGTTTGGTGATGATGGGTTGGAATGAAGTATAAATTTGATATTCCAAATGGGAATTTGATTCTTGTTTCTCTGTTCTAATAATGGCAGTCCTTCTTCGTAGCTGGAGGAACCTATCCTGGGTCAGTTCTTGCCGGATTCGACCAGGATACATTAGCTACAGCATTTAATGTGAGAAATTCGACTAAAATCCCTAATCTGTCTCTCTAATTTCCAAACATCAATGAAATTTCTCACAATTCAATGCACTCCACAGGTTTCTTATACAGAATTGAGGAGGATTCTATCAAGACAACGACAGGGGCCAATCGTTTACGTCTCAGATACTGAAGCGCCTAGGGTTTGGAGCAAGTTTCTGCAAGTGAAAGATGAAGCCAGACTAAATAAAGTAGCTGATATCAATGAAGATGGTGAAGAATCGGAGAAAAATAAGCGATGGTCGTGGAGAAAGCTCATGGATTCGATCTTTAGAAACGAAAATCGGGACAAAAGTAAAAAAATAACAAGAACGGGAAAATCCCCCGATTCATACAATCTCTACGACAAAACCCCAGACTTCAGCAACGCCTACGGATGGAGCGTTGCCCTAGACGAAACCGAGTACCATCCTCTAGGCCACTCCGGAATCGGAGTTTATCTAGTCAATCTCACAGCGGTAAATCTAGTTTTTCAAATCCCTAATTCTGCTATCAATTAGATCAATTAGTTCATGTATCTTCTATGATGTGCGAATCACTACGAATTAACTCAAATACAGGGATCAATGATGGCACCGCACGTGAATCCAACGGCAGCGGAGTACGGAATCGTCCTCAAAGGCACAGGTACAATACAAATCGTGTATCCAAACGGAACCTCCGCGATGAACGCGGAAGTAACCGAAGGAGACGTGTTCTGGATTCCAAGATACTTCCCATTCTGTCAAATCGCATCTCGAACAGGTCCGTTCGAGTTCTTTGGATTCACGACGTCGGCGCGGAAGAATCGGCCGCAGTTCTTGGCGGGAGCGAGTTCGATTTTCCACACTTTGAGAAGCATGGAGATGGCGACGGCTTTTGACATAACAGAGGATGATATGGATAGGTTGCTGGGGGCGCAGTATGAGGCGATAATTCTGCCGTCGGCGGAGATAGCGCCGCCGCACAAGGAAGAGGAGAAGAAGAGGAGGAAAGAGGAAGAGAGGAGGGAAGCGGAAACGGAAACGGAACGGGAAAGAGAAAGGGAAAGGGAAAGGGAAAGAAGAGTGAAAGAAGCGGTTCGAAGCTTTGAAAGGAATTTGGTTACGGGATTTGTTTAGTTAAGAGTTGAAATGGCGGGAAATAAACTATAATTTCCATTGCTTTTTTTTTTTTTTAACGGTCTGCTTCGTTTTGGTTTAGGGTTTGTTCATGAGGTTGGAGATTTGTGAATAAAACCCATAACTTTTTACTGCA

mRNA sequence

AAGAAACAGAAGCAAAGATGGGGAAGAAAGAAGCCCTTTTGATACTGCTGATTGTTGCGGTTTTGGGAAATGCAATTGGGATTAAGGAGGAAGAAGAAGAAGAATGGTGGAGAAAGAGAGAAGAAGAGAAAGAGTTTGGAAGGAAAGAAAGGTTTCTAATGGAGGACTCGAAGAAGGTGATAGAGACAGAAGCAGGAGAAATGAGGGTTATGAGAAGTCCAGCTTCAAGAATTCTTGACAGGCCCATGCATATTGGCTTCATCACCATGGAACCTAAATCCTTGTTTGTCCCTCAGTATTTGGACTCCACCTTGATTCTCTTCGTCCGTAGAGGGGATGTGAAAGTGGGATTGATTTACAAAGATGAGTTAGCAGAGAGGAGGATGAAGGGTGGGGACGTGTTCCGAATCCCAGCCGGTTCAGTATTTTACATGGTGAATGTAGGGGAAGGACAGAGACTTCAGATAATCTGCAGCATTGATAAATCTGAGAGTTTGAGTTATGGTACTTTCCAGTCCTTCTTCGTAGCTGGAGGAACCTATCCTGGGTCAGTTCTTGCCGGATTCGACCAGGATACATTAGCTACAGCATTTAATGTTTCTTATACAGAATTGAGGAGGATTCTATCAAGACAACGACAGGGGCCAATCGTTTACGTCTCAGATACTGAAGCGCCTAGGGTTTGGAGCAAGTTTCTGCAAGTGAAAGATGAAGCCAGACTAAATAAAGTAGCTGATATCAATGAAGATGGTGAAGAATCGGAGAAAAATAAGCGATGGTCGTGGAGAAAGCTCATGGATTCGATCTTTAGAAACGAAAATCGGGACAAAAGTAAAAAAATAACAAGAACGGGAAAATCCCCCGATTCATACAATCTCTACGACAAAACCCCAGACTTCAGCAACGCCTACGGATGGAGCGTTGCCCTAGACGAAACCGAGTACCATCCTCTAGGCCACTCCGGAATCGGAGTTTATCTAGTCAATCTCACAGCGGGATCAATGATGGCACCGCACGTGAATCCAACGGCAGCGGAGTACGGAATCGTCCTCAAAGGCACAGGTACAATACAAATCGTGTATCCAAACGGAACCTCCGCGATGAACGCGGAAGTAACCGAAGGAGACGTGTTCTGGATTCCAAGATACTTCCCATTCTGTCAAATCGCATCTCGAACAGGTCCGTTCGAGTTCTTTGGATTCACGACGTCGGCGCGGAAGAATCGGCCGCAGTTCTTGGCGGGAGCGAGTTCGATTTTCCACACTTTGAGAAGCATGGAGATGGCGACGGCTTTTGACATAACAGAGGATGATATGGATAGGTTGCTGGGGGCGCAGTATGAGGCGATAATTCTGCCGTCGGCGGAGATAGCGCCGCCGCACAAGGAAGAGGAGAAGAAGAGGAGGAAAGAGGAAGAGAGGAGGGAAGCGGAAACGGAAACGGAACGGGAAAGAGAAAGGGAAAGGGAAAGGGAAAGAAGAGTGAAAGAAGCGGTTCGAAGCTTTGAAAGGAATTTGGTTACGGGATTTGTTTAGTTAAGAGTTGAAATGGCGGGAAATAAACTATAATTTCCATTGCTTTTTTTTTTTTTTAACGGTCTGCTTCGTTTTGGTTTAGGGTTTGTTCATGAGGTTGGAGATTTGTGAATAAAACCCATAACTTTTTACTGCA

Coding sequence (CDS)

ATGGGGAAGAAAGAAGCCCTTTTGATACTGCTGATTGTTGCGGTTTTGGGAAATGCAATTGGGATTAAGGAGGAAGAAGAAGAAGAATGGTGGAGAAAGAGAGAAGAAGAGAAAGAGTTTGGAAGGAAAGAAAGGTTTCTAATGGAGGACTCGAAGAAGGTGATAGAGACAGAAGCAGGAGAAATGAGGGTTATGAGAAGTCCAGCTTCAAGAATTCTTGACAGGCCCATGCATATTGGCTTCATCACCATGGAACCTAAATCCTTGTTTGTCCCTCAGTATTTGGACTCCACCTTGATTCTCTTCGTCCGTAGAGGGGATGTGAAAGTGGGATTGATTTACAAAGATGAGTTAGCAGAGAGGAGGATGAAGGGTGGGGACGTGTTCCGAATCCCAGCCGGTTCAGTATTTTACATGGTGAATGTAGGGGAAGGACAGAGACTTCAGATAATCTGCAGCATTGATAAATCTGAGAGTTTGAGTTATGGTACTTTCCAGTCCTTCTTCGTAGCTGGAGGAACCTATCCTGGGTCAGTTCTTGCCGGATTCGACCAGGATACATTAGCTACAGCATTTAATGTTTCTTATACAGAATTGAGGAGGATTCTATCAAGACAACGACAGGGGCCAATCGTTTACGTCTCAGATACTGAAGCGCCTAGGGTTTGGAGCAAGTTTCTGCAAGTGAAAGATGAAGCCAGACTAAATAAAGTAGCTGATATCAATGAAGATGGTGAAGAATCGGAGAAAAATAAGCGATGGTCGTGGAGAAAGCTCATGGATTCGATCTTTAGAAACGAAAATCGGGACAAAAGTAAAAAAATAACAAGAACGGGAAAATCCCCCGATTCATACAATCTCTACGACAAAACCCCAGACTTCAGCAACGCCTACGGATGGAGCGTTGCCCTAGACGAAACCGAGTACCATCCTCTAGGCCACTCCGGAATCGGAGTTTATCTAGTCAATCTCACAGCGGGATCAATGATGGCACCGCACGTGAATCCAACGGCAGCGGAGTACGGAATCGTCCTCAAAGGCACAGGTACAATACAAATCGTGTATCCAAACGGAACCTCCGCGATGAACGCGGAAGTAACCGAAGGAGACGTGTTCTGGATTCCAAGATACTTCCCATTCTGTCAAATCGCATCTCGAACAGGTCCGTTCGAGTTCTTTGGATTCACGACGTCGGCGCGGAAGAATCGGCCGCAGTTCTTGGCGGGAGCGAGTTCGATTTTCCACACTTTGAGAAGCATGGAGATGGCGACGGCTTTTGACATAACAGAGGATGATATGGATAGGTTGCTGGGGGCGCAGTATGAGGCGATAATTCTGCCGTCGGCGGAGATAGCGCCGCCGCACAAGGAAGAGGAGAAGAAGAGGAGGAAAGAGGAAGAGAGGAGGGAAGCGGAAACGGAAACGGAACGGGAAAGAGAAAGGGAAAGGGAAAGGGAAAGAAGAGTGAAAGAAGCGGTTCGAAGCTTTGAAAGGAATTTGGTTACGGGATTTGTTTAG

Protein sequence

MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVADINEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETERERERERERERRVKEAVRSFERNLVTGFV
Homology
BLAST of PI0019216 vs. ExPASy Swiss-Prot
Match: Q9SK09 (Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=At2g28490 PE=2 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 2.2e-133
Identity = 237/428 (55.37%), Postives = 318/428 (74.30%), Query Frame = 0

Query: 41  GRKERFLMEDSKKVIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLI 100
           GR+  F+M +S++VI++E GEMRV+ SP  RI+++PMHIGF+TMEPK+LFVPQYLDS+L+
Sbjct: 81  GRRGWFMMRESRQVIKSEGGEMRVVLSPRGRIIEKPMHIGFLTMEPKTLFVPQYLDSSLL 140

Query: 101 LFVRRGDVKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESL 160
           +F+R+G+  +G+I KDE  ER++K GD++ IPAGSVFY+ N G GQRL +ICSID ++SL
Sbjct: 141 IFIRQGEATLGVICKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQRLHVICSIDPTQSL 200

Query: 161 SYGTFQSFFVAGGTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAP 220
            + TFQ F++ GG  P SVLAGFD  TL +AFNVS  EL++++  Q +GPIVYV++   P
Sbjct: 201 GFETFQPFYIGGG--PSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQP 260

Query: 221 R----VWSKFLQVKDEARLNKVADINEDGEESEKNKR----WSWRKLMDSIFRNENRDKS 280
           +    VW++FL ++ E +  ++  + E  + S ++++    WSWR ++ SI   +  ++ 
Sbjct: 261 QPQSTVWTQFLGLRGEEKHKQLKKLLETKQGSPQDQQYSSGWSWRNIVRSIL--DLTEEK 320

Query: 281 KKITRTGKSPDSYNLYDK--TPDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMM 340
            K + + +  DSYN+YDK   P F N YGWS+ALD  +Y PL HSGIGVYLVNLTAG+MM
Sbjct: 321 NKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDDYKPLKHSGIGVYLVNLTAGAMM 380

Query: 341 APHVNPTAAEYGIVLKGTGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPF 400
           APH+NPTA EYGIVL G+G IQ+V+PNGTSAMN  V+ GDVFWIPRYF FCQIASRTGPF
Sbjct: 381 APHMNPTATEYGIVLAGSGEIQVVFPNGTSAMNTRVSVGDVFWIPRYFAFCQIASRTGPF 440

Query: 401 EFFGFTTSARKNRPQFLAGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSAE 459
           EF GFTTSA KNRPQFL G++S+  TL    ++ AF + E+ M R + AQ EA+ILP+  
Sbjct: 441 EFVGFTTSAHKNRPQFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPA 500

BLAST of PI0019216 vs. ExPASy Swiss-Prot
Match: F4IQK5 (Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=At2g18540 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.1e-44
Identity = 150/476 (31.51%), Postives = 230/476 (48.32%), Query Frame = 0

Query: 54  VIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLI 113
           V+ TE G +  ++      +    HI FIT+EP +L +P  L S ++ FV  G   +  I
Sbjct: 53  VVATEFGNISAVQ------IGDGYHIQFITLEPNALLLPLLLHSDMVFFVHTGTGILNWI 112

Query: 114 YKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKS-ESLSYGTFQSFFVAG 173
            ++   +  ++ GDVFR+ +G+VFY V+  E  R+  I ++ K       G + S     
Sbjct: 113 DEESERKLELRRGDVFRLRSGTVFY-VHSNEKLRVYAIFNVGKCLNDPCLGAYSSV---- 172

Query: 174 GTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDE 233
                 +L GFD  TL +AF V    LR+I    +   IV       PR           
Sbjct: 173 ----RDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIV----NALPR----------- 232

Query: 234 ARLNKVADINEDGEESEKNKRW-SWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKT 293
              N+   + ED  +S   + + S   + D +      D +KK +RT      +N++++ 
Sbjct: 233 ---NRTQGLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKSRT------FNVFEED 292

Query: 294 PDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTI 353
           PDF N  G S+ +DE +   L  S  GV++VNLT GSM+ PH NP+A E  IVL+G G +
Sbjct: 293 PDFENNNGRSIVVDEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMV 352

Query: 354 QIVYPNGTSAMNAE-------VTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRP 413
           ++V     S+   +       V EGDVF +P++ P  Q++     F F GF+TSA+ N P
Sbjct: 353 RVVNQQSLSSCKNDRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHP 412

Query: 414 QFLAGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSA--------------- 473
           QFL G SS+   L    +A +F+++ + +  LL AQ E++I   A               
Sbjct: 413 QFLVGQSSVLKVLDRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIE 472

Query: 474 --------EIAPPHKEEEKKRRKEEERREAETETERERERERERERRVKEAVRSFE 498
                   EI    KEEE+ R++EE +R  E E +R  E E ER++R +E  R  E
Sbjct: 473 ERKRREEEEIERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKRE 489

BLAST of PI0019216 vs. ExPASy Swiss-Prot
Match: F4JQG6 (Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=At4g36700 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.9e-40
Identity = 150/511 (29.35%), Postives = 245/511 (47.95%), Query Frame = 0

Query: 8   LILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAGEMRVMRS 67
           ++LL++  L      K EE EE+         F        +  K + ET+ G++  ++ 
Sbjct: 11  VLLLVLLFLCTESLAKSEESEEYDVAVPSCCGFSSPLLIKKDQWKPIFETKFGQISTVQI 70

Query: 68  PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAERRMKGGD 127
                   P  I  IT+EP ++ +P  L S ++ FV  G   +  +  +E     ++ GD
Sbjct: 71  GNGCGGMGPYKIHSITLEPNTILLPLLLHSDMVFFVDSGSGILNWV-DEEAKSTEIRLGD 130

Query: 128 VFRIPAGSVFYM----VNVGEGQRLQIICSIDKSESL----SYGTFQSFFVAGGTYPGSV 187
           V+R+  GSVFY+    V++  G +L++      ++       +G + S           +
Sbjct: 131 VYRLRPGSVFYLQSKPVDIFLGTKLKLYAIFSNNDECLHDPCFGAYSSI--------TDL 190

Query: 188 LAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVA 247
           + GFD+  L +AF V    +   L R R  P + VS+T      +   Q+  + RL K+ 
Sbjct: 191 MFGFDETILQSAFGVPEGIIE--LMRNRTKPPLIVSETLCTPGVANTWQL--QPRLLKLF 250

Query: 248 DINEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYG 307
             + D              L+D+  + E ++K +K+    K   ++N+++  PDF + YG
Sbjct: 251 AGSAD--------------LVDNKKKKEKKEKKEKV----KKAKTFNVFESEPDFESPYG 310

Query: 308 WSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGT 367
            ++ ++  +   L  S +GV +VNLT GSMM PH NP A E  IVLKG G ++++  + +
Sbjct: 311 RTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKGAGMVRVLRSSIS 370

Query: 368 SAMNAE-------VTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASS 427
           S  ++E       V EGD+F +PR  P  Q++       F GFTTSA+ N PQFLAG  S
Sbjct: 371 SNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGEDS 430

Query: 428 IFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILP------------SAEIAPPHKEEEK 487
               L    +A + +++   +D LLGAQ EA+IL               EI     ++E+
Sbjct: 431 ALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKKIDDER 490

Query: 488 KRRKEE---ERREAETETERERERERERERR 489
           KRR +E   E  EA+ E E  R+RE E E++
Sbjct: 491 KRRHDERKKEEEEAKREEEERRKREEEEEKK 490

BLAST of PI0019216 vs. ExPASy Swiss-Prot
Match: V9VGU0 (Vicilin Pin k 2.0101 OS=Pinus koraiensis OX=88728 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 5.8e-33
Identity = 120/453 (26.49%), Postives = 199/453 (43.93%), Query Frame = 0

Query: 17  GNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAGEMRVMRS--PASRILD 76
           G+  G +EEE       REE       +RF +  S     +EAGE+R + +    S +L+
Sbjct: 52  GHHRGGREEE-------REENPYVFHSDRFRIRAS-----SEAGEIRALPNFGEVSELLE 111

Query: 77  --RPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAERRMKGGDVFRIP 136
                 +  I M+P ++ +P Y+D+  IL+V  G   +  + ++EL +R+++ GDVF +P
Sbjct: 112 GISRYRVTCIEMKPNTVMLPHYIDAKWILYVTGGRGYIAYVQQNELVKRKLEEGDVFGVP 171

Query: 137 AGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVLAGFDQDTLATAF 196
           +G  FY+VN  +   L+ I S+ ++ES   G ++ F+VAGG  P +V + F  D L  AF
Sbjct: 172 SGHTFYLVNNDDHNSLR-IASLLRTESTMRGEYEPFYVAGGRNPETVYSAFSDDVLEAAF 231

Query: 197 NVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVADINEDGEESEKNK 256
           N    +L  I    R+G I Y ++           Q+++  R                  
Sbjct: 232 NTDVQKLEHIFGAHRRGVIFYANEE----------QIREMMRRGGF-------------- 291

Query: 257 RWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGWSVALDETEYHPL 316
                              S + T   + P  +NL ++ PDF N  G        +   L
Sbjct: 292 -------------------SAESTSASEQPKPFNLRNQKPDFENDNGRFTRAGPKDNPFL 351

Query: 317 GHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYP-----NGTSA------ 376
               + V    L  G+M AP  N  A    IV++G G I++  P     +G S+      
Sbjct: 352 DSVDVTVGFGVLNPGTMTAPSHNTKATSIAIVMEGEGRIEMACPHLGQEHGWSSPRERGH 411

Query: 377 -------MNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIF 436
                  + A +  G V+ +P   P  +IAS  G  E   F  +   N  +FLAG +++ 
Sbjct: 412 QDINYERVRARLRTGTVYVVPAGHPITEIASTNGRLEILWFDINTSGNEREFLAGKNNVL 448

Query: 437 HTLRSMEMATAFDITE-DDMDRLLGAQYEAIIL 447
            TL       +F+I   ++++ +L AQ + +IL
Sbjct: 472 QTLEKEVRHLSFNIPRGEEIEEVLQAQKDQVIL 448

BLAST of PI0019216 vs. ExPASy Swiss-Prot
Match: B4X640 (Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 1.8e-29
Identity = 116/440 (26.36%), Postives = 182/440 (41.36%), Query Frame = 0

Query: 24  EEEEEEWWRKREEEKEFGRKERFLMEDSKKV--IETEAGEMRVM-----RSPASRILDRP 83
           EEEEEE   ++EEE E      ++ ED      ++TE G++ V+     RS   R L++ 
Sbjct: 137 EEEEEEGDEEQEEEDE----NPYVFEDEHFTTRVKTEQGKVVVLPKFTKRSKLLRGLEK- 196

Query: 84  MHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAERRMKGGDVFRIPAGSV 143
             + F+   P++  VP ++D+  I FV  G   +  I +++     +K GD+ RI AG+ 
Sbjct: 197 YRLAFLVANPQAFVVPNHMDADSIFFVSWGRGTITKIRENKRESMNVKQGDIIRIRAGTP 256

Query: 144 FYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVLAGFDQDTLATAFNVSY 203
           FY+VN  E ++L I+  +        G ++ F   GG  P S    F ++ L  A     
Sbjct: 257 FYIVNTDENEKLYIVKLLQPVN--LPGHYEVFHGPGGENPESFYRAFSREVLEAALKTPR 316

Query: 204 TELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVADINEDGEESEKNKRWSW 263
            +L ++  +Q +G IV  S  +                      I       E    W +
Sbjct: 317 DKLEKLFEKQDEGAIVKASKEQ----------------------IRAMSRRGEGPSIWPF 376

Query: 264 RKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGWSVALDETEYHPLGHSG 323
                                TGKS  ++NL+ K P  SN YG     +  +Y PL    
Sbjct: 377 ---------------------TGKSTGTFNLFKKDPSQSNNYGQLFESEFKDYPPLQELD 436

Query: 324 IGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTSAMN------------- 383
           I V  VN+T G M  P  N  A +  IV+ G G ++I  P+ +S+ N             
Sbjct: 437 IMVSYVNITKGGMSGPFYNSRATKIAIVVSGEGRLEIACPHLSSSKNSGQEKSGPSYKKL 496

Query: 384 -AEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSMEM 443
            + +    VF +P   PF  +AS     E   F  +A  N    LAG  +I   +     
Sbjct: 497 SSSIRTDSVFVVPAGHPFVTVASGNQNLEILCFEVNAEGNIRYTLAGKKNIIEVMEKEAK 526

BLAST of PI0019216 vs. ExPASy TrEMBL
Match: A0A0A0KHQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290870 PE=4 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.8e-264
Identity = 479/508 (94.29%), Postives = 491/508 (96.65%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKKEALLILLIVAVLGNAIGIKEEEEEEWWR+REEEK FG KERFLM DSKKVIETEAG
Sbjct: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEK-FGSKERFLMVDSKKVIETEAG 60

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMR P SRILD+ MHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE
Sbjct: 61  EMRVMRGPISRILDKAMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQSFFVAGG YPGSVL
Sbjct: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 180

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTELRRILSRQRQGPIVY+SDTE+PRVWSKFLQVKD+ARL+KVAD
Sbjct: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYISDTESPRVWSKFLQVKDKARLSKVAD 240

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
            NEDGEESEKNKRWSWRKLM+SIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW
Sbjct: 241 NNEDGEESEKNKRWSWRKLMNSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVL+GTGTIQIVYPNGTS
Sbjct: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLRGTGTIQIVYPNGTS 360

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTS+RKNRPQFLAGASSIFHTLR+M
Sbjct: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGASSIFHTLRNM 420

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETET----E 480
           EMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETET    E
Sbjct: 421 EMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETETEWE 480

Query: 481 RERERERERERRVKEAVRSFERNLVTGF 505
           RERERERERERRV E V SF RNLV GF
Sbjct: 481 RERERERERERRVDEVVGSFGRNLVMGF 507

BLAST of PI0019216 vs. ExPASy TrEMBL
Match: A0A5D3CSE7 (Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00510 PE=4 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.1e-263
Identity = 476/504 (94.44%), Postives = 489/504 (97.02%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKKEALLILLIVAVLGNAIGIKEEEEEEWWR+REEE+EFGRKERFLM DSKKVIETEAG
Sbjct: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEREFGRKERFLMVDSKKVIETEAG 60

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMR PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE
Sbjct: 61  EMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQSFFVAGG YPGSVL
Sbjct: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 180

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+PRVWSKFLQV DEARL+KVAD
Sbjct: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFLQVNDEARLSKVAD 240

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
           I+EDGE+SEKNK WSWRKL++SIFRN NRDKSKKITRTGKSPDSYNLYDK PDFSNAYGW
Sbjct: 241 IDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKKITRTGKSPDSYNLYDKDPDFSNAYGW 300

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPL HSGIGVYLVNLTAGSMMAPHVNPTA+EYGIVL+GTGTIQIVYPNGTS
Sbjct: 301 SVALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRGTGTIQIVYPNGTS 360

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM
Sbjct: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETERERE 480
           EMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEERREA  E ERERE
Sbjct: 421 EMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEERREA--EWERERE 480

Query: 481 RERERERRVKEAVRSFERNLVTGF 505
           RERERERRV E VRSF RNLV GF
Sbjct: 481 RERERERRVDEMVRSFGRNLVMGF 502

BLAST of PI0019216 vs. ExPASy TrEMBL
Match: A0A1S3CKT5 (LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g28490 OS=Cucumis melo OX=3656 GN=LOC103501928 PE=4 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.0e-261
Identity = 475/504 (94.25%), Postives = 488/504 (96.83%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKKEALLILLIVAVLGNAIGIKEEEEEEWWR+REEE+EFGRKERFLM DSKKVIETEAG
Sbjct: 43  MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEREFGRKERFLMVDSKKVIETEAG 102

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMR PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE
Sbjct: 103 EMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 162

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQSFFVAGG YPGSVL
Sbjct: 163 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 222

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+PRVWSKFLQV DEARL+KVAD
Sbjct: 223 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFLQVNDEARLSKVAD 282

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
           I+EDGE+SEKNK WSWRKL++SIFRN NRDKSKK TRTGKSPDSYNLYDK PDFSNAYGW
Sbjct: 283 IDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKK-TRTGKSPDSYNLYDKDPDFSNAYGW 342

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPL HSGIGVYLVNLTAGSMMAPHVNPTA+EYGIVL+GTGTIQIVYPNGTS
Sbjct: 343 SVALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRGTGTIQIVYPNGTS 402

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM
Sbjct: 403 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 462

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETERERE 480
           EMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEERREA  E ERERE
Sbjct: 463 EMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEERREA--EWERERE 522

Query: 481 RERERERRVKEAVRSFERNLVTGF 505
           RERERERRV E VRSF RNLV GF
Sbjct: 523 RERERERRVDEMVRSFGRNLVMGF 543

BLAST of PI0019216 vs. ExPASy TrEMBL
Match: A0A5A7SVK6 (Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G001130 PE=4 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.1e-239
Identity = 431/457 (94.31%), Postives = 442/457 (96.72%), Query Frame = 0

Query: 48  MEDSKKVIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGD 107
           M DSKKVIETEAGEMRVMR PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGD
Sbjct: 1   MVDSKKVIETEAGEMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGD 60

Query: 108 VKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQS 167
           VKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQS
Sbjct: 61  VKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQS 120

Query: 168 FFVAGGTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFL 227
           FFVAGG YPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+PRVWSKFL
Sbjct: 121 FFVAGGKYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFL 180

Query: 228 QVKDEARLNKVADINEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNL 287
           QV DEARL+KVADI+EDGE+SEKNK WSWRKL++SIFRN NRDKSKKITRTGKSPDSYNL
Sbjct: 181 QVNDEARLSKVADIDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKKITRTGKSPDSYNL 240

Query: 288 YDKTPDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKG 347
           YDK PDFSNAYGWSVALDETEYHPL HSGIGVYLVNLTAGSMMAPHVNPTA+EYGIVL+G
Sbjct: 241 YDKDPDFSNAYGWSVALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRG 300

Query: 348 TGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFL 407
           TGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFL
Sbjct: 301 TGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFL 360

Query: 408 AGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEE 467
           AGASSIFHTLRSMEMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEE
Sbjct: 361 AGASSIFHTLRSMEMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEE 420

Query: 468 RREAETETERERERERERERRVKEAVRSFERNLVTGF 505
           RREA  E ERERERERERERRV E VRSF RNLV GF
Sbjct: 421 RREA--EWERERERERERERRVDEMVRSFGRNLVMGF 455

BLAST of PI0019216 vs. ExPASy TrEMBL
Match: A0A6J1FRY9 (vicilin-like seed storage protein At2g28490 OS=Cucurbita moschata OX=3662 GN=LOC111448275 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.9e-238
Identity = 429/492 (87.20%), Postives = 461/492 (93.70%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKE----EEEEEWWRKREEEKEFGRKERFLMEDSKKVIE 60
           MGKKEAL++LLI+AVLGNAIGIKE    EEEEEWWR+REEE+EF  KERFL+EDSK+VIE
Sbjct: 1   MGKKEALVMLLIIAVLGNAIGIKEEAEAEEEEEWWREREEEREFRSKERFLLEDSKRVIE 60

Query: 61  TEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKD 120
           TEAGEMRV+RSPASRILDRPMHIGFITMEPKSLFVPQYLDS+LILFVRRG+VKVGLIYKD
Sbjct: 61  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 120

Query: 121 ELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYP 180
           ELAERRMKGGDV+RIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFV GGTYP
Sbjct: 121 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVGGGTYP 180

Query: 181 GSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLN 240
            SVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+P VWSKFLQVKD  + N
Sbjct: 181 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGEKGN 240

Query: 241 KVADINEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSN 300
           ++++INE+GEE+EKNK WSWR LM SIF NENRDK+K+ TRTGKSPDSYNLYDKTPDFSN
Sbjct: 241 QISNINENGEEAEKNKAWSWRNLMSSIFGNENRDKTKR-TRTGKSPDSYNLYDKTPDFSN 300

Query: 301 AYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYP 360
           AYGWSVALDE EY PLGHSGIGVYLVNLTAGSMMAPH+NPTAAEYGIVL+GTGTIQIVYP
Sbjct: 301 AYGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYP 360

Query: 361 NGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHT 420
           NGTSAM+ EVTEGDVFW+PRYFPFCQIASRTGPFEFFGFTTS+RKNRPQFLAGA+SIFHT
Sbjct: 361 NGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGANSIFHT 420

Query: 421 LRSMEMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETE 480
           LRS  +ATAFDITEDD+DRLL  QYEA+ILPSAEIAPPHKEEEK+RR+EE RRE E E E
Sbjct: 421 LRSSAVATAFDITEDDLDRLLSLQYEAVILPSAEIAPPHKEEEKRRRREEGRREREREGE 480

Query: 481 RERERERERERR 489
           RERERE E  RR
Sbjct: 481 REREREEEWTRR 491

BLAST of PI0019216 vs. NCBI nr
Match: XP_004149563.1 (vicilin-like seed storage protein At2g28490 [Cucumis sativus])

HSP 1 Score: 920.6 bits (2378), Expect = 5.8e-264
Identity = 479/508 (94.29%), Postives = 491/508 (96.65%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKKEALLILLIVAVLGNAIGIKEEEEEEWWR+REEEK FG KERFLM DSKKVIETEAG
Sbjct: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEK-FGSKERFLMVDSKKVIETEAG 60

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMR P SRILD+ MHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE
Sbjct: 61  EMRVMRGPISRILDKAMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQSFFVAGG YPGSVL
Sbjct: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 180

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTELRRILSRQRQGPIVY+SDTE+PRVWSKFLQVKD+ARL+KVAD
Sbjct: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYISDTESPRVWSKFLQVKDKARLSKVAD 240

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
            NEDGEESEKNKRWSWRKLM+SIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW
Sbjct: 241 NNEDGEESEKNKRWSWRKLMNSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVL+GTGTIQIVYPNGTS
Sbjct: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLRGTGTIQIVYPNGTS 360

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTS+RKNRPQFLAGASSIFHTLR+M
Sbjct: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGASSIFHTLRNM 420

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETET----E 480
           EMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETET    E
Sbjct: 421 EMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETETEWE 480

Query: 481 RERERERERERRVKEAVRSFERNLVTGF 505
           RERERERERERRV E V SF RNLV GF
Sbjct: 481 RERERERERERRVDEVVGSFGRNLVMGF 507

BLAST of PI0019216 vs. NCBI nr
Match: TYK14322.1 (vicilin-like seed storage protein [Cucumis melo var. makuwa])

HSP 1 Score: 918.7 bits (2373), Expect = 2.2e-263
Identity = 476/504 (94.44%), Postives = 489/504 (97.02%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKKEALLILLIVAVLGNAIGIKEEEEEEWWR+REEE+EFGRKERFLM DSKKVIETEAG
Sbjct: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEREFGRKERFLMVDSKKVIETEAG 60

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMR PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE
Sbjct: 61  EMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQSFFVAGG YPGSVL
Sbjct: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 180

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+PRVWSKFLQV DEARL+KVAD
Sbjct: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFLQVNDEARLSKVAD 240

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
           I+EDGE+SEKNK WSWRKL++SIFRN NRDKSKKITRTGKSPDSYNLYDK PDFSNAYGW
Sbjct: 241 IDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKKITRTGKSPDSYNLYDKDPDFSNAYGW 300

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPL HSGIGVYLVNLTAGSMMAPHVNPTA+EYGIVL+GTGTIQIVYPNGTS
Sbjct: 301 SVALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRGTGTIQIVYPNGTS 360

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM
Sbjct: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETERERE 480
           EMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEERREA  E ERERE
Sbjct: 421 EMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEERREA--EWERERE 480

Query: 481 RERERERRVKEAVRSFERNLVTGF 505
           RERERERRV E VRSF RNLV GF
Sbjct: 481 RERERERRVDEMVRSFGRNLVMGF 502

BLAST of PI0019216 vs. NCBI nr
Match: XP_008463915.1 (PREDICTED: LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g28490 [Cucumis melo])

HSP 1 Score: 912.1 bits (2356), Expect = 2.1e-261
Identity = 475/504 (94.25%), Postives = 488/504 (96.83%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKKEALLILLIVAVLGNAIGIKEEEEEEWWR+REEE+EFGRKERFLM DSKKVIETEAG
Sbjct: 43  MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEREFGRKERFLMVDSKKVIETEAG 102

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMR PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE
Sbjct: 103 EMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 162

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQSFFVAGG YPGSVL
Sbjct: 163 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 222

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+PRVWSKFLQV DEARL+KVAD
Sbjct: 223 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFLQVNDEARLSKVAD 282

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
           I+EDGE+SEKNK WSWRKL++SIFRN NRDKSKK TRTGKSPDSYNLYDK PDFSNAYGW
Sbjct: 283 IDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKK-TRTGKSPDSYNLYDKDPDFSNAYGW 342

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPL HSGIGVYLVNLTAGSMMAPHVNPTA+EYGIVL+GTGTIQIVYPNGTS
Sbjct: 343 SVALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRGTGTIQIVYPNGTS 402

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM
Sbjct: 403 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 462

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETERERE 480
           EMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEERREA  E ERERE
Sbjct: 463 EMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEERREA--EWERERE 522

Query: 481 RERERERRVKEAVRSFERNLVTGF 505
           RERERERRV E VRSF RNLV GF
Sbjct: 523 RERERERRVDEMVRSFGRNLVMGF 543

BLAST of PI0019216 vs. NCBI nr
Match: XP_038901798.1 (vicilin-like seed storage protein At2g28490 [Benincasa hispida])

HSP 1 Score: 882.9 bits (2280), Expect = 1.3e-252
Identity = 456/505 (90.30%), Postives = 480/505 (95.05%), Query Frame = 0

Query: 1   MGKKEALLILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAG 60
           MGKK+ALL+LLI+AVLG AIGIKEE EEEWWR    E+EFGRKERFLMEDSK+VIETEAG
Sbjct: 37  MGKKDALLLLLIIAVLGKAIGIKEEGEEEWWR----EREFGRKERFLMEDSKQVIETEAG 96

Query: 61  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 120
           EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDS+LILFV RGDVKVGL+YKDELAE
Sbjct: 97  EMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVHRGDVKVGLVYKDELAE 156

Query: 121 RRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVL 180
           RRMK GDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYP SVL
Sbjct: 157 RRMKAGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPSSVL 216

Query: 181 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVAD 240
           AGFDQDTLATAFNVSYTEL+RILSRQRQGPIVYVSDTE+PRVWSKFLQVKDEARL+K+AD
Sbjct: 217 AGFDQDTLATAFNVSYTELKRILSRQRQGPIVYVSDTESPRVWSKFLQVKDEARLSKIAD 276

Query: 241 INEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGW 300
           I+EDGEE+EKN+ WSWRKLM SIF NENRDKSKK T TGKSPDSYNLYDK PDFSNAYGW
Sbjct: 277 IDEDGEEAEKNEPWSWRKLMVSIFGNENRDKSKK-TTTGKSPDSYNLYDKNPDFSNAYGW 336

Query: 301 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTS 360
           SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVL+GTGTIQIVYPNGTS
Sbjct: 337 SVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLRGTGTIQIVYPNGTS 396

Query: 361 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSM 420
           AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTS+RKNRPQFLAGASSIFHTLRS+
Sbjct: 397 AMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGASSIFHTLRSL 456

Query: 421 EMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEERREAETETERERE 480
           EMATAFDITE+D++RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEERR      ERERE
Sbjct: 457 EMATAFDITEEDLERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEERR------ERERE 516

Query: 481 RERERERRVKEAVRSFERNLVTGFV 506
           RE E ERRV EA+RSFERN+V G+V
Sbjct: 517 REPETERRVDEAIRSFERNMVMGYV 530

BLAST of PI0019216 vs. NCBI nr
Match: KAA0035314.1 (vicilin-like seed storage protein [Cucumis melo var. makuwa])

HSP 1 Score: 835.9 bits (2158), Expect = 1.9e-238
Identity = 431/457 (94.31%), Postives = 442/457 (96.72%), Query Frame = 0

Query: 48  MEDSKKVIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGD 107
           M DSKKVIETEAGEMRVMR PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGD
Sbjct: 1   MVDSKKVIETEAGEMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGD 60

Query: 108 VKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQS 167
           VKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRL+IICSIDKSESLSYGTFQS
Sbjct: 61  VKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQS 120

Query: 168 FFVAGGTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFL 227
           FFVAGG YPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTE+PRVWSKFL
Sbjct: 121 FFVAGGKYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFL 180

Query: 228 QVKDEARLNKVADINEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNL 287
           QV DEARL+KVADI+EDGE+SEKNK WSWRKL++SIFRN NRDKSKKITRTGKSPDSYNL
Sbjct: 181 QVNDEARLSKVADIDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKKITRTGKSPDSYNL 240

Query: 288 YDKTPDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKG 347
           YDK PDFSNAYGWSVALDETEYHPL HSGIGVYLVNLTAGSMMAPHVNPTA+EYGIVL+G
Sbjct: 241 YDKDPDFSNAYGWSVALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRG 300

Query: 348 TGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFL 407
           TGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFL
Sbjct: 301 TGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFL 360

Query: 408 AGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSAEIAPPHKEEEKKRRKEEE 467
           AGASSIFHTLRSMEMATAFDITEDDM+RLLGAQYEAIILPSAEIAPPHKEEEK+RRKEEE
Sbjct: 361 AGASSIFHTLRSMEMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEE 420

Query: 468 RREAETETERERERERERERRVKEAVRSFERNLVTGF 505
           RREA  E ERERERERERERRV E VRSF RNLV GF
Sbjct: 421 RREA--EWERERERERERERRVDEMVRSFGRNLVMGF 455

BLAST of PI0019216 vs. TAIR 10
Match: AT2G28490.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.6e-134
Identity = 237/428 (55.37%), Postives = 318/428 (74.30%), Query Frame = 0

Query: 41  GRKERFLMEDSKKVIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLI 100
           GR+  F+M +S++VI++E GEMRV+ SP  RI+++PMHIGF+TMEPK+LFVPQYLDS+L+
Sbjct: 81  GRRGWFMMRESRQVIKSEGGEMRVVLSPRGRIIEKPMHIGFLTMEPKTLFVPQYLDSSLL 140

Query: 101 LFVRRGDVKVGLIYKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKSESL 160
           +F+R+G+  +G+I KDE  ER++K GD++ IPAGSVFY+ N G GQRL +ICSID ++SL
Sbjct: 141 IFIRQGEATLGVICKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQRLHVICSIDPTQSL 200

Query: 161 SYGTFQSFFVAGGTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAP 220
            + TFQ F++ GG  P SVLAGFD  TL +AFNVS  EL++++  Q +GPIVYV++   P
Sbjct: 201 GFETFQPFYIGGG--PSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQP 260

Query: 221 R----VWSKFLQVKDEARLNKVADINEDGEESEKNKR----WSWRKLMDSIFRNENRDKS 280
           +    VW++FL ++ E +  ++  + E  + S ++++    WSWR ++ SI   +  ++ 
Sbjct: 261 QPQSTVWTQFLGLRGEEKHKQLKKLLETKQGSPQDQQYSSGWSWRNIVRSIL--DLTEEK 320

Query: 281 KKITRTGKSPDSYNLYDK--TPDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMM 340
            K + + +  DSYN+YDK   P F N YGWS+ALD  +Y PL HSGIGVYLVNLTAG+MM
Sbjct: 321 NKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDDYKPLKHSGIGVYLVNLTAGAMM 380

Query: 341 APHVNPTAAEYGIVLKGTGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPF 400
           APH+NPTA EYGIVL G+G IQ+V+PNGTSAMN  V+ GDVFWIPRYF FCQIASRTGPF
Sbjct: 381 APHMNPTATEYGIVLAGSGEIQVVFPNGTSAMNTRVSVGDVFWIPRYFAFCQIASRTGPF 440

Query: 401 EFFGFTTSARKNRPQFLAGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSAE 459
           EF GFTTSA KNRPQFL G++S+  TL    ++ AF + E+ M R + AQ EA+ILP+  
Sbjct: 441 EFVGFTTSAHKNRPQFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPA 500

BLAST of PI0019216 vs. TAIR 10
Match: AT2G18540.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 182.6 bits (462), Expect = 8.0e-46
Identity = 150/476 (31.51%), Postives = 230/476 (48.32%), Query Frame = 0

Query: 54  VIETEAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLI 113
           V+ TE G +  ++      +    HI FIT+EP +L +P  L S ++ FV  G   +  I
Sbjct: 53  VVATEFGNISAVQ------IGDGYHIQFITLEPNALLLPLLLHSDMVFFVHTGTGILNWI 112

Query: 114 YKDELAERRMKGGDVFRIPAGSVFYMVNVGEGQRLQIICSIDKS-ESLSYGTFQSFFVAG 173
            ++   +  ++ GDVFR+ +G+VFY V+  E  R+  I ++ K       G + S     
Sbjct: 113 DEESERKLELRRGDVFRLRSGTVFY-VHSNEKLRVYAIFNVGKCLNDPCLGAYSSV---- 172

Query: 174 GTYPGSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDE 233
                 +L GFD  TL +AF V    LR+I    +   IV       PR           
Sbjct: 173 ----RDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIV----NALPR----------- 232

Query: 234 ARLNKVADINEDGEESEKNKRW-SWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKT 293
              N+   + ED  +S   + + S   + D +      D +KK +RT      +N++++ 
Sbjct: 233 ---NRTQGLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKSRT------FNVFEED 292

Query: 294 PDFSNAYGWSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTI 353
           PDF N  G S+ +DE +   L  S  GV++VNLT GSM+ PH NP+A E  IVL+G G +
Sbjct: 293 PDFENNNGRSIVVDEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMV 352

Query: 354 QIVYPNGTSAMNAE-------VTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRP 413
           ++V     S+   +       V EGDVF +P++ P  Q++     F F GF+TSA+ N P
Sbjct: 353 RVVNQQSLSSCKNDRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHP 412

Query: 414 QFLAGASSIFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILPSA--------------- 473
           QFL G SS+   L    +A +F+++ + +  LL AQ E++I   A               
Sbjct: 413 QFLVGQSSVLKVLDRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIE 472

Query: 474 --------EIAPPHKEEEKKRRKEEERREAETETERERERERERERRVKEAVRSFE 498
                   EI    KEEE+ R++EE +R  E E +R  E E ER++R +E  R  E
Sbjct: 473 ERKRREEEEIERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKRE 489

BLAST of PI0019216 vs. TAIR 10
Match: AT4G36700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 2.0e-41
Identity = 150/511 (29.35%), Postives = 245/511 (47.95%), Query Frame = 0

Query: 8   LILLIVAVLGNAIGIKEEEEEEWWRKREEEKEFGRKERFLMEDSKKVIETEAGEMRVMRS 67
           ++LL++  L      K EE EE+         F        +  K + ET+ G++  ++ 
Sbjct: 11  VLLLVLLFLCTESLAKSEESEEYDVAVPSCCGFSSPLLIKKDQWKPIFETKFGQISTVQI 70

Query: 68  PASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAERRMKGGD 127
                   P  I  IT+EP ++ +P  L S ++ FV  G   +  +  +E     ++ GD
Sbjct: 71  GNGCGGMGPYKIHSITLEPNTILLPLLLHSDMVFFVDSGSGILNWV-DEEAKSTEIRLGD 130

Query: 128 VFRIPAGSVFYM----VNVGEGQRLQIICSIDKSESL----SYGTFQSFFVAGGTYPGSV 187
           V+R+  GSVFY+    V++  G +L++      ++       +G + S           +
Sbjct: 131 VYRLRPGSVFYLQSKPVDIFLGTKLKLYAIFSNNDECLHDPCFGAYSSI--------TDL 190

Query: 188 LAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVA 247
           + GFD+  L +AF V    +   L R R  P + VS+T      +   Q+  + RL K+ 
Sbjct: 191 MFGFDETILQSAFGVPEGIIE--LMRNRTKPPLIVSETLCTPGVANTWQL--QPRLLKLF 250

Query: 248 DINEDGEESEKNKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYG 307
             + D              L+D+  + E ++K +K+    K   ++N+++  PDF + YG
Sbjct: 251 AGSAD--------------LVDNKKKKEKKEKKEKV----KKAKTFNVFESEPDFESPYG 310

Query: 308 WSVALDETEYHPLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGT 367
            ++ ++  +   L  S +GV +VNLT GSMM PH NP A E  IVLKG G ++++  + +
Sbjct: 311 RTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKGAGMVRVLRSSIS 370

Query: 368 SAMNAE-------VTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASS 427
           S  ++E       V EGD+F +PR  P  Q++       F GFTTSA+ N PQFLAG  S
Sbjct: 371 SNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGEDS 430

Query: 428 IFHTLRSMEMATAFDITEDDMDRLLGAQYEAIILP------------SAEIAPPHKEEEK 487
               L    +A + +++   +D LLGAQ EA+IL               EI     ++E+
Sbjct: 431 ALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKKIDDER 490

Query: 488 KRRKEE---ERREAETETERERERERERERR 489
           KRR +E   E  EA+ E E  R+RE E E++
Sbjct: 491 KRRHDERKKEEEEAKREEEERRKREEEEEKK 490

BLAST of PI0019216 vs. TAIR 10
Match: AT2G28680.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-09
Identity = 77/352 (21.88%), Postives = 139/352 (39.49%), Query Frame = 0

Query: 82  ITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKD-ELAERRMKGGDVFRIPAGSVFYMV 141
           + +E   L +P+Y DS  + +V +G    G++  + E     +K GD   +P G V +  
Sbjct: 42  LALEKYGLALPRYSDSPKVAYVLQGAGTAGIVLPEKEEKVIAIKKGDSIALPFGVVTWWF 101

Query: 142 NVGEGQRLQIICSIDKSESLSYGTFQSFFVAGGTYPGSVLAGFDQDTLATAFNVSYTELR 201
           N  E   L ++   +  +    G F  F++ G      +  GF  + +  A+++  T ++
Sbjct: 102 N-NEDTELVVLFLGETHKGHKAGQFTDFYLTGS---NGIFTGFSTEFVGRAWDLDETTVK 161

Query: 202 RILSRQRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVADINEDGEESEKNKRWSWRKLM 261
           +++  Q    IV V                         D +    E +K          
Sbjct: 162 KLVGSQTGNGIVKV-------------------------DASLKMPEPKKG--------- 221

Query: 262 DSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGWSVALDETEYHPL-GHSGIGV 321
                    D+   +    ++P   ++ D         G  V +  T+  PL G  G G 
Sbjct: 222 ---------DRKGFVLNCLEAPLDVDIKD---------GGRVVVLNTKNLPLVGEVGFGA 281

Query: 322 YLVNLTAGSMMAPHVN-PTAAEYGIVLKGTGTIQIVYPNGTSAMNAEVTEGDVFWIPRYF 381
            LV +   SM +P  +  +A +   ++ G+G +QIV  +G   +   V  G +F +PR+F
Sbjct: 282 DLVRIDGHSMCSPGFSCDSALQVTYIVGGSGRVQIVGADGKRVLETHVKAGVLFIVPRFF 333

Query: 382 PFCQIASRTGPFEFFGFTTSARKNRPQF--LAGASSIFHTLRSMEMATAFDI 429
              +IA   G   +F   T+     P F  LAG +S++  L    +  AF +
Sbjct: 342 VVSKIADSDG-LSWFSIVTTP---DPIFTHLAGRTSVWKALSPEVLQAAFKV 333

BLAST of PI0019216 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 3.5e-09
Identity = 88/435 (20.23%), Postives = 160/435 (36.78%), Query Frame = 0

Query: 58  EAGEMRVMRSPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYK-- 117
           EAG+M V    +  +    + +  IT++P S+F+P +     + +V +G+  +G I    
Sbjct: 53  EAGQMEVWDHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGC 112

Query: 118 -DELAE------------------------RRMKGGDVFRIPAGSVFYMVNVGEGQR-LQ 177
            +  AE                           + GDVF   AG   +  N G+    + 
Sbjct: 113 PETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIV 172

Query: 178 IICSIDKSESLSYGTFQSFFVAGG---------TYP--GSVLAGFDQDTLATAFNVSYTE 237
           I+  +   E+      + F +AG          T+P   +  +GFD + +A AF ++   
Sbjct: 173 IVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIET 232

Query: 238 LRRILSR--------QRQGPIVYVSDTEAPRVWSKFLQVKDEARLNKVADINEDGEESEK 297
            +++ ++        +  GP+ +V     PR W                   +DG  +  
Sbjct: 233 AKQLQNQKDNRGNIIRANGPLHFV--IPPPREW------------------QQDGIANGI 292

Query: 298 NKRWSWRKLMDSIFRNENRDKSKKITRTGKSPDSYNLYDKTPDFSNAYGWSVALDETEYH 357
            + +   K+ ++I   E  D                       FS   G    L+     
Sbjct: 293 EETYCTAKIHENIDDPERSD----------------------HFSTRAGRISTLNSLNLP 352

Query: 358 PLGHSGIGVYLVNLTAGSMMAPHVNPTAAEYGIVLKGTGTIQIVYPNGTSAMNAEVTEGD 417
            L    +      L +G M+ P     A     V  G   IQ+V  NG S  N +V +G 
Sbjct: 353 VLRLVRLNALRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVDDNGQSVFNEQVGQGQ 412

Query: 418 VFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSMEMATAFDITE 446
           +  IP+ F   + A  TG FE+  F T+        L+G +S    +    +  ++ + E
Sbjct: 413 IIVIPQGFAVSKTAGETG-FEWISFKTNDNA-YINTLSGQTSYLRAVPVDVIKASYGVNE 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SK092.2e-13355.37Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
F4IQK51.1e-4431.51Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
F4JQG62.9e-4029.35Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
V9VGU05.8e-3326.49Vicilin Pin k 2.0101 OS=Pinus koraiensis OX=88728 PE=1 SV=1[more]
B4X6401.8e-2926.36Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KHQ12.8e-26494.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290870 PE=4 SV=1[more]
A0A5D3CSE71.1e-26394.44Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CKT51.0e-26194.25LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g28490 OS=Cucumis melo... [more]
A0A5A7SVK69.1e-23994.31Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1FRY95.9e-23887.20vicilin-like seed storage protein At2g28490 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_004149563.15.8e-26494.29vicilin-like seed storage protein At2g28490 [Cucumis sativus][more]
TYK14322.12.2e-26394.44vicilin-like seed storage protein [Cucumis melo var. makuwa][more]
XP_008463915.12.1e-26194.25PREDICTED: LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g28490 [Cuc... [more]
XP_038901798.11.3e-25290.30vicilin-like seed storage protein At2g28490 [Benincasa hispida][more]
KAA0035314.11.9e-23894.31vicilin-like seed storage protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G28490.11.6e-13455.37RmlC-like cupins superfamily protein [more]
AT2G18540.18.0e-4631.51RmlC-like cupins superfamily protein [more]
AT4G36700.12.0e-4129.35RmlC-like cupins superfamily protein [more]
AT2G28680.11.2e-0921.88RmlC-like cupins superfamily protein [more]
AT1G03890.13.5e-0920.23RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 455..499
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 452..489
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 448..489
NoneNo IPR availablePANTHERPTHR31189:SF2OS03G0336100 PROTEINcoord: 2..456
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 2..456
NoneNo IPR availableCDDcd02245cupin_7S_vicilin-like_Ccoord: 292..443
e-value: 8.45416E-61
score: 195.043
NoneNo IPR availableCDDcd02244cupin_7S_vicilin-like_Ncoord: 54..215
e-value: 6.47665E-76
score: 234.71
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 285..434
e-value: 3.5E-49
score: 179.4
coord: 44..200
e-value: 5.5E-20
score: 82.4
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 52..196
e-value: 7.2E-10
score: 38.6
coord: 285..430
e-value: 3.6E-33
score: 114.3
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 277..449
e-value: 1.3E-49
score: 170.2
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 34..226
e-value: 1.7E-53
score: 182.9
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 50..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0019216.1PI0019216.1mRNA