PI0019164 (gene) Melon (PI 482460) v1

Overview
NamePI0019164
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic-like
Locationchr11: 4750987 .. 4753222 (-)
RNA-Seq ExpressionPI0019164
SyntenyPI0019164
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATAATTTTCACTCTCTATTATCTCTCTTTGCTCTTCCCTCTCCCCACCTAATTTTAAGGAGAGGTTTTGTAGGTACACTTGTATTACGCATCTTCCGACACAAGTTTTCTTCGGTGCAAAGGTAAACTTCCATCCTTCATAACTACTCAACTTTCGTGTTTGTTTTTGTATAATTTTTTCTTATGTTCAAACATATTTAAGATATATATTTAATCAACTATGACTTAGTTATACTCTCCTAATACTTCTCATACTCATATAGACATTATTATGAAGAATATTGCAAGGACCAACAAGTAATTCTTGATATTACTTGATGATGTTGATAATATACAAAACCTTTTGTGTTTTAAATTACTGTCTTAACCTATAAAATTAAGTGTATTATAATATTTTGTTAGTGATTTAAAAATAATTTAATGCTAGGGTGTTCACAAAAGTCAAGCATATGCTTCCAATTTATTCACTATTCAAAATTACATAAGAATTAATACAAACTCAAATACTTCATATAAAAATAATAATTAATGAGAGCAAGTCATTTTGTTTAATTAGTACAATAGATTCATTATTCACAACACATTATTACATGACAAAGGAAGTGTGTAAAAATTGATTTTATGAACTGTGTCGTAAATCATCTTAACAATATTTATTGAGTTAAAATGAATCATATAACATATAATTTACTATCTTAATAGTATATTATAACTCACTCAATATTAGTTTTTTTATTATACTAAAATTATTTTTAAACTTTTATTATCTTATGTATTGACTAATTTATAATTATCCTTTCTCAACTCAAATTAAAATAATCTACATTAATTCCAAATACAACTCAAACTCAAATAGACTACATCCATCGAAGAATTTAGTATAGGGTCTGAGATTTTTCCTCAACTTTATACTCCAACTTGATACTCTTTATATATATTAATATATAACTTAAATAATATAAATAACAGTAACTAGTCTATTGGTAAAATTGTTTTTCAGCTCCTTTAGACGAATTACAATTCCCACAGTTTACAGAAAAACTAATAAAACCATTAGTTATAATAATCCATTTATACCATTCCAAACAAACCTGAAAATTAAAAGGATTATGAATATTTATTTTATATGTAAATGAATTTTTTATTTTTGTTGTAATAAACTTTTACAGGATCAATATATCGTGAGTTAAAAGAATGAAAATTTCGTTATCGACGAGCTGCAAGCAAGTTGCGGTGGAGCCGCCGCTTGTCAACTTTCAAGGCACAAACAACACACTGAATTTGGAACCATTTTTCCGGCGAAAAGACAAAGTGGTATTTGTAATGGGAGCTACCGGTACAGGGAAATCTCGGTTGGCAATTGATTTAGCCACTCGTTTCCCAGCTGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCAAAATCCTCACCAATAAAGTCACTGAAAAAGAATGCCGTGGGGTCCCACATCATCTCCTTGGAATCGCCGATCCCAATTCAAACTTCACTGTCGCCGACTTCCGCCGCCATGCCTCATCGGCGATCAACTCCATTATTGTAGGAAAACGTCGGCTCCCTGTCCTGGCTGGTGGCTCCAACTCCTTCATCGAATCACTTGTAGAGGAGGAGCCTGAGTTTCGTTACCGTTATGATTGTTGCTTTTTATGGGTGGATGTTTCATTACCCATCCTCAACTCGTTTGTGTCGGATCGTGTTGATCGGATGTTGGAAAATGGATTAGTCAATGAGGTACGAAATTTATTTGATCCTAATGGATTAGGAAATGATTATGGACGTGGAATTCGACGCGCCATTGGAGTGCCGGAACTTGACGCGTTTCTACGCACTGAGATGGATTCTACCATTGATGATAAAACTCGATCTCGAATCCTTAAAGCGGCTATTTTGAAAATAAAAGAAAATACAATAAAATTGGCGTGTCGTCAACGACAAAAGATTCACCGCCTTCAAAACAAATGGCGGGGATGGAATAATCTCCACCGTATCGATGCTACTGAAGTGTTCTTACAACTCGGCAACAGAGACTATTCGTTTGATAATGCTTGGGAGGCGCTTGTGGTGGAGCCAAGCTCCATAATCGTTGACCAATTTCTCTCTGAAAATAGTTTCAAACTTACAGAAAAGGTGTTCAACGCAGTTTTTTGAATAATTTTTTTCAACTTTAAAGATATATTACTCGTCCAAATGATGACACATGACAGCAAC

mRNA sequence

ATATAATTTTCACTCTCTATTATCTCTCTTTGCTCTTCCCTCTCCCCACCTAATTTTAAGGAGAGGTTTTGTAGGTACACTTGTATTACGCATCTTCCGACACAAGTTTTCTTCGGTGCAAAGGATCAATATATCGTGAGTTAAAAGAATGAAAATTTCGTTATCGACGAGCTGCAAGCAAGTTGCGGTGGAGCCGCCGCTTGTCAACTTTCAAGGCACAAACAACACACTGAATTTGGAACCATTTTTCCGGCGAAAAGACAAAGTGGTATTTGTAATGGGAGCTACCGGTACAGGGAAATCTCGGTTGGCAATTGATTTAGCCACTCGTTTCCCAGCTGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCAAAATCCTCACCAATAAAGTCACTGAAAAAGAATGCCGTGGGGTCCCACATCATCTCCTTGGAATCGCCGATCCCAATTCAAACTTCACTGTCGCCGACTTCCGCCGCCATGCCTCATCGGCGATCAACTCCATTATTGTAGGAAAACGTCGGCTCCCTGTCCTGGCTGGTGGCTCCAACTCCTTCATCGAATCACTTGTAGAGGAGGAGCCTGAGTTTCGTTACCGTTATGATTGTTGCTTTTTATGGGTGGATGTTTCATTACCCATCCTCAACTCGTTTGTGTCGGATCGTGTTGATCGGATGTTGGAAAATGGATTAGTCAATGAGGTACGAAATTTATTTGATCCTAATGGATTAGGAAATGATTATGGACGTGGAATTCGACGCGCCATTGGAGTGCCGGAACTTGACGCGTTTCTACGCACTGAGATGGATTCTACCATTGATGATAAAACTCGATCTCGAATCCTTAAAGCGGCTATTTTGAAAATAAAAGAAAATACAATAAAATTGGCGTGTCGTCAACGACAAAAGATTCACCGCCTTCAAAACAAATGGCGGGGATGGAATAATCTCCACCGTATCGATGCTACTGAAGTGTTCTTACAACTCGGCAACAGAGACTATTCGTTTGATAATGCTTGGGAGGCGCTTGTGGTGGAGCCAAGCTCCATAATCGTTGACCAATTTCTCTCTGAAAATAGTTTCAAACTTACAGAAAAGGTGTTCAACGCAGTTTTTTGAATAATTTTTTTCAACTTTAAAGATATATTACTCGTCCAAATGATGACACATGACAGCAAC

Coding sequence (CDS)

ATGAAAATTTCGTTATCGACGAGCTGCAAGCAAGTTGCGGTGGAGCCGCCGCTTGTCAACTTTCAAGGCACAAACAACACACTGAATTTGGAACCATTTTTCCGGCGAAAAGACAAAGTGGTATTTGTAATGGGAGCTACCGGTACAGGGAAATCTCGGTTGGCAATTGATTTAGCCACTCGTTTCCCAGCTGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCAAAATCCTCACCAATAAAGTCACTGAAAAAGAATGCCGTGGGGTCCCACATCATCTCCTTGGAATCGCCGATCCCAATTCAAACTTCACTGTCGCCGACTTCCGCCGCCATGCCTCATCGGCGATCAACTCCATTATTGTAGGAAAACGTCGGCTCCCTGTCCTGGCTGGTGGCTCCAACTCCTTCATCGAATCACTTGTAGAGGAGGAGCCTGAGTTTCGTTACCGTTATGATTGTTGCTTTTTATGGGTGGATGTTTCATTACCCATCCTCAACTCGTTTGTGTCGGATCGTGTTGATCGGATGTTGGAAAATGGATTAGTCAATGAGGTACGAAATTTATTTGATCCTAATGGATTAGGAAATGATTATGGACGTGGAATTCGACGCGCCATTGGAGTGCCGGAACTTGACGCGTTTCTACGCACTGAGATGGATTCTACCATTGATGATAAAACTCGATCTCGAATCCTTAAAGCGGCTATTTTGAAAATAAAAGAAAATACAATAAAATTGGCGTGTCGTCAACGACAAAAGATTCACCGCCTTCAAAACAAATGGCGGGGATGGAATAATCTCCACCGTATCGATGCTACTGAAGTGTTCTTACAACTCGGCAACAGAGACTATTCGTTTGATAATGCTTGGGAGGCGCTTGTGGTGGAGCCAAGCTCCATAATCGTTGACCAATTTCTCTCTGAAAATAGTTTCAAACTTACAGAAAAGGTGTTCAACGCAGTTTTTTGA

Protein sequence

MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAINSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVEPSSIIVDQFLSENSFKLTEKVFNAVF
Homology
BLAST of PI0019164 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 349.0 bits (894), Expect = 5.9e-95
Identity = 188/306 (61.44%), Postives = 236/306 (77.12%), Query Frame = 0

Query: 7   TSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI 66
           T+ +QV ++P  +NFQG  N +++ PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI
Sbjct: 6   TALRQV-IQPLSLNFQG--NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI 65

Query: 67  INSDKIQVYKGLKILTNKVTEKECRGVPHHLLG-IADPNSNFTVADFRRHASSAINSIIV 126
           +NSDKIQVYKGL I+TNKVT +E  GVPHHLLG + D   +FT  DF+R A  A+ S IV
Sbjct: 66  VNSDKIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVES-IV 125

Query: 127 GKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGL 186
            + R+P++AGGSNS+IE+LV +  +FR RY+CCFLWVDVS P+L+SFVS+RVD+M++ GL
Sbjct: 126 QRDRVPIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGL 185

Query: 187 VNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAILKIKE 246
           V+EVR +FDP+   +DY  GIRRAIGVPELD FLR+EM      +T  R+L+ AI KIKE
Sbjct: 186 VDEVRRIFDPS--SSDYSAGIRRAIGVPELDEFLRSEM-RNYPAETTERLLETAIEKIKE 245

Query: 247 NTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVEPSSII 306
           NT  LACRQ QKI RL  +W+ W N+HR+DATEVFL+ G      D AW+  V  PS++ 
Sbjct: 246 NTCLLACRQLQKIQRLYKQWK-W-NMHRVDATEVFLRRGE---EADEAWDNSVAHPSALA 298

Query: 307 VDQFLS 312
           V++FLS
Sbjct: 306 VEKFLS 298

BLAST of PI0019164 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 322.0 bits (824), Expect = 7.7e-87
Identity = 177/316 (56.01%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MK S+S S KQV    P++ F+   + +N+  F   K+KV+FVMGATG+GKSRLAIDLAT
Sbjct: 1   MKFSIS-SLKQV---QPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNS-NFTVADFRRHASSA 120
           RF  EIINSDKIQ+YKGL +LTNKVT KECRGVPHHLLG+ D  + N T   + R AS A
Sbjct: 61  RFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQA 120

Query: 121 INSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRY-RYDCCFLWVDVSLPILNSFVSDRVD 180
           I S +    +LP++AGGSNS+IE+LV     F    YDCCF+WVDVSLP+LNSFVS RVD
Sbjct: 121 I-SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVD 180

Query: 181 RMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKA 240
           RM+E GL+ EVR +F+P     +Y  GIRRAIGVPEL  +LR E  S +D  T+S++L  
Sbjct: 181 RMMEAGLLEEVREVFNPKA---NYSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDV 240

Query: 241 AILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALV 300
           A+  IK+NT  LACRQ +KI RL  KW+   ++HR+DATEVFL+    +   D AWE LV
Sbjct: 241 AVKNIKKNTEILACRQLKKIQRLHKKWK--MSMHRVDATEVFLKRNVEEQ--DEAWENLV 300

Query: 301 VEPSSIIVDQFLSENS 315
             PS  IVD+F + N+
Sbjct: 301 ARPSERIVDKFYNNNN 302

BLAST of PI0019164 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.2e-78
Identity = 158/314 (50.32%), Postives = 221/314 (70.38%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTN---NTLNLEPFFRRKDKVVFVMGATGTGKSRLAID 60
           MKIS++  CKQ     P ++F       N L L P +  KDKVV +MGATGTGKSRL++D
Sbjct: 3   MKISMA-MCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHAS 120
           +ATRF AEIINSDKIQV++GL I+TNK+T +E  GVPHHLLG+  P ++ T A++   A+
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 SAINSIIVGKRRLPVLAGGSNSFIESLVEE-EPEFRYRYDCCFLWVDVSLPILNSFVSDR 180
            +I S++  + +LP++ GGSNS++E+LV++ E +FR RYDCCFLWVDV+LP+L+ FVS+R
Sbjct: 123 LSIESVL-NRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSER 182

Query: 181 VDRMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRIL 240
           VD+M+E+G+V EVR  FD +   +DY RGI++AIG PE D F R E    ++D  R  +L
Sbjct: 183 VDKMVESGMVEEVREFFDFS--NSDYSRGIKKAIGFPEFDRFFRNEQFLNVED--REELL 242

Query: 241 KAAILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEA 300
              + +IK NT +LACRQR+KI RL+ K + W ++ R+DAT VF +  ++    + AWE 
Sbjct: 243 SKVLEEIKRNTFELACRQREKIERLR-KVKKW-SIQRVDATPVFTKRRSK-MDANVAWER 302

Query: 301 LVVEPSSIIVDQFL 311
           LV  PS+  V +FL
Sbjct: 303 LVAGPSTDTVSRFL 306

BLAST of PI0019164 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.4e-59
Identity = 141/318 (44.34%), Postives = 181/318 (56.92%), Query Frame = 0

Query: 24  TNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKILTN 83
           T   ++L     RK+K++ +MGATGTGKSRL+IDLA  FP E+INSDK+QVYKGL I TN
Sbjct: 16  TTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTN 75

Query: 84  KVTEKECRGVPHHLLGIADP-NSNFTVADFRRHASSAINSIIVGKRRLPVLAGGSNSFIE 143
           K++  +  GVPHHLLG  DP     T ADFR  A  A+ S I G+R+LPVL GGSNSFI 
Sbjct: 76  KISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAV-SEITGRRKLPVLVGGSNSFIH 135

Query: 144 SLVEEE----------------PEFRYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGLV 203
           +L+ +                      RYDCCFLWVDVS+ +L  +++ RVD MLE G+ 
Sbjct: 136 ALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMF 195

Query: 204 NEVRNLFDPNGLGNDYG----RGIRRAIGVPELDAFLRTEMDSTID------DKTRSRIL 263
           +E+   + P    +D       G+R+AIGVPE D +        ++      D+ R    
Sbjct: 196 DELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAF 255

Query: 264 KAAILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRD--YSFDNAW 313
           + A+  IKENT  LA RQ  KI RL  K  GW +L R+DATE F      D        W
Sbjct: 256 EEAVRAIKENTCHLAKRQIGKILRL--KGAGW-DLRRLDATESFRAAMTSDSGEKCTEIW 315

BLAST of PI0019164 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 227.3 bits (578), Expect = 2.6e-58
Identity = 128/299 (42.81%), Postives = 185/299 (61.87%), Query Frame = 0

Query: 36  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPH 95
           RKDKVV ++GATG GKSRL++DLATRFP+EIINSDKIQVY+GL+I TN++T ++ RGVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 96  HLLGIADP-NSNFTVADFRRHASSAINSIIVGKRRLPVLAGGSNSFIESLVEE--EPEF- 155
           HLLG+ +P +   T  +FR  AS+ +   I  ++++P++AGGSNSF+ +L+ +  +P+F 
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKE-ITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 182

Query: 156 -----------RYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGLVNEVRNLFDPNGLGN 215
                        RY+CCF+WVDVS  +L  ++  RVD M+++G+  E+   +DP   G 
Sbjct: 183 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 242

Query: 216 DYGRGIRRAIGVPELDAFLR---TEMDSTIDDKTRSRILKAAILKIKENTIKLACRQRQK 275
           +   GIR+AIGVPE D + +    E      D  R      A+  IK NT  LA RQ +K
Sbjct: 243 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 302

Query: 276 IHRLQNKWRGWNNLHRIDATEVF---LQLGNRDYSFDNAWEALVVEPSSIIVDQFLSEN 314
           I  L++   GW  + R+DAT  F   +   + +  +   WE  V+EPS  IV + L +N
Sbjct: 303 IEMLKD--AGW-EIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of PI0019164 vs. ExPASy TrEMBL
Match: A0A5A7STV9 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00780 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 309/328 (94.21%), Postives = 317/328 (96.65%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGT-NNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60
           MKI LSTSCKQVAVEPPLVNFQGT NNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120
           TRFPAEIINSDKIQVYKGLK+LTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDR 180
           INSIIVGKRRLP+LAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVS+RVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAA 240
           MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLR EMDST DDK RSRILKAA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGN-RDYSFDNAWEALV 300
           ILKIKENTIKLACRQ+QKIHRLQNKWRGWNN HRIDATE FLQLGN  +YS DNAWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 VEPSSIIVDQFLSENSFKLTEKVFNAVF 327
           VEPSS+I+DQFLS+NSFKLTEKVFNAVF
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAVF 328

BLAST of PI0019164 vs. ExPASy TrEMBL
Match: A0A1S3C845 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103497923 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 309/328 (94.21%), Postives = 317/328 (96.65%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGT-NNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60
           MKI LSTSCKQVAVEPPLVNFQGT NNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120
           TRFPAEIINSDKIQVYKGLK+LTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDR 180
           INSIIVGKRRLP+LAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVS+RVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAA 240
           MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLR EMDST DDK RSRILKAA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGN-RDYSFDNAWEALV 300
           ILKIKENTIKLACRQ+QKIHRLQNKWRGWNN HRIDATE FLQLGN  +YS DNAWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 VEPSSIIVDQFLSENSFKLTEKVFNAVF 327
           VEPSS+I+DQFLS+NSFKLTEKVFNAVF
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAVF 328

BLAST of PI0019164 vs. ExPASy TrEMBL
Match: A0A0A0KHK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.2e-172
Identity = 303/326 (92.94%), Postives = 313/326 (96.01%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MKISLSTSCKQ+AVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT
Sbjct: 1   MKISLSTSCKQIAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQVYKGLK+LTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASS I
Sbjct: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECCGVPHHLLGIADPNSNFTVADFRRHASSTI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NSI+VGKR LP+LAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVS+RVDRM
Sbjct: 121 NSIVVGKRCLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           LENGLVNEVR+LFDPNGLGNDYG GIRRAIGVPELDAFLR EMDS  DDKTRSRILKAAI
Sbjct: 181 LENGLVNEVRSLFDPNGLGNDYGHGIRRAIGVPELDAFLRAEMDSATDDKTRSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKENTIKLACRQRQKIHRLQ+KWRGWNNLHRIDATEVFLQL + DYS DNAWE LVV+
Sbjct: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWETLVVK 300

Query: 301 PSSIIVDQFLSENSFKLTEKVFNAVF 327
           PS  IVDQFLSENSFKLTEKVF+AVF
Sbjct: 301 PSFKIVDQFLSENSFKLTEKVFSAVF 326

BLAST of PI0019164 vs. ExPASy TrEMBL
Match: A0A6J1H3C2 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460117 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.9e-141
Identity = 266/325 (81.85%), Postives = 284/325 (87.38%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQVA EPPLVN QGT N LNL+PFFR+KDK+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGT-NILNLKPFFRQKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL+ILTNKVTEKEC GVPHHLLGIADPNSNFT  DFRRHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NS IVGK RLP++AGGSNSFIESLV++E EFRYRYDCCFLWVDVSLP+L+SFVSDRVDRM
Sbjct: 121 NS-IVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           L+NGLVNEVRNLFDP    NDY RGIRRAIGVPELDAFL  EMDS  DDK RSRIL AAI
Sbjct: 181 LDNGLVNEVRNLFDPK--ENDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKEN+IKLA RQRQKIHRLQ+KW GW NLHRIDATEVFLQ    +YS D+AWEALV E
Sbjct: 241 LKIKENSIKLALRQRQKIHRLQSKW-GW-NLHRIDATEVFLQHDGGEYS-DHAWEALVAE 300

Query: 301 PSSIIVDQFLSENSFKLTEKVFNAV 326
           PSSIIVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAGKIPMAV 318

BLAST of PI0019164 vs. ExPASy TrEMBL
Match: A0A6J1KZJ7 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499016 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 6.2e-140
Identity = 263/325 (80.92%), Postives = 283/325 (87.08%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQ A E PLVNFQGT N LNL+PFFRRKDK+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSASCKQAATESPLVNFQGT-NILNLKPFFRRKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL+ILTNKVTEKEC GVPHHLLGI DPNSNFT  DF RHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIVDPNSNFTATDFCRHASLAI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NS IVGK RLP++AGGSNSFIESLV+ E EFRYRYDCCFLWVDVSLP+L+SFVSDRVDRM
Sbjct: 121 NS-IVGKHRLPIIAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           L+NGLVNEVRNLFDP   GNDY RGIRRAIGVPELDAFLR EMDS  D+K RS+IL+AAI
Sbjct: 181 LDNGLVNEVRNLFDPK--GNDYTRGIRRAIGVPELDAFLRAEMDSATDNKARSQILQAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKEN+IKLA RQRQKIH LQ+KW GW NLHRIDATEVFLQ G  +YS ++AWEALV E
Sbjct: 241 LKIKENSIKLALRQRQKIHHLQSKW-GW-NLHRIDATEVFLQHGGGEYS-EHAWEALVAE 300

Query: 301 PSSIIVDQFLSENSFKLTEKVFNAV 326
           PSSIIVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAAKIPMAV 318

BLAST of PI0019164 vs. NCBI nr
Match: XP_008458554.1 (PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo] >KAA0033426.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 621.3 bits (1601), Expect = 4.7e-174
Identity = 309/328 (94.21%), Postives = 317/328 (96.65%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGT-NNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60
           MKI LSTSCKQVAVEPPLVNFQGT NNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120
           TRFPAEIINSDKIQVYKGLK+LTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDR 180
           INSIIVGKRRLP+LAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVS+RVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAA 240
           MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLR EMDST DDK RSRILKAA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGN-RDYSFDNAWEALV 300
           ILKIKENTIKLACRQ+QKIHRLQNKWRGWNN HRIDATE FLQLGN  +YS DNAWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 VEPSSIIVDQFLSENSFKLTEKVFNAVF 327
           VEPSS+I+DQFLS+NSFKLTEKVFNAVF
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAVF 328

BLAST of PI0019164 vs. NCBI nr
Match: XP_004150320.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN47261.1 hypothetical protein Csa_017001 [Cucumis sativus])

HSP 1 Score: 615.5 bits (1586), Expect = 2.6e-172
Identity = 303/326 (92.94%), Postives = 313/326 (96.01%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MKISLSTSCKQ+AVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT
Sbjct: 1   MKISLSTSCKQIAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQVYKGLK+LTNKVTEKEC GVPHHLLGIADPNSNFTVADFRRHASS I
Sbjct: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECCGVPHHLLGIADPNSNFTVADFRRHASSTI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NSI+VGKR LP+LAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVS+RVDRM
Sbjct: 121 NSIVVGKRCLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           LENGLVNEVR+LFDPNGLGNDYG GIRRAIGVPELDAFLR EMDS  DDKTRSRILKAAI
Sbjct: 181 LENGLVNEVRSLFDPNGLGNDYGHGIRRAIGVPELDAFLRAEMDSATDDKTRSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKENTIKLACRQRQKIHRLQ+KWRGWNNLHRIDATEVFLQL + DYS DNAWE LVV+
Sbjct: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWETLVVK 300

Query: 301 PSSIIVDQFLSENSFKLTEKVFNAVF 327
           PS  IVDQFLSENSFKLTEKVF+AVF
Sbjct: 301 PSFKIVDQFLSENSFKLTEKVFSAVF 326

BLAST of PI0019164 vs. NCBI nr
Match: XP_038875913.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 544.3 bits (1401), Expect = 7.3e-151
Identity = 281/333 (84.38%), Postives = 299/333 (89.79%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MKISLS SCKQ A EPPLVNFQG +NTLNLEPFFRRKDKVVFVMGATG+GKSRLAIDLAT
Sbjct: 1   MKISLSASCKQAAAEPPLVNFQG-SNTLNLEPFFRRKDKVVFVMGATGSGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQVYKGL+ILTNKVTEKEC GVPHHLLGIADPNSNFT ADFRRHASSAI
Sbjct: 61  RFPAEIINSDKIQVYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NS IVGKRRLP++AGGSNSFIESLV++EPEFRYRYDCCFLWVDVSLP+L+SFVS+RVDRM
Sbjct: 121 NS-IVGKRRLPIVAGGSNSFIESLVDDEPEFRYRYDCCFLWVDVSLPVLHSFVSERVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           L++GLVNEVRNLFDP   GNDY RGIRRAIGVPELDAFL  EMDS  DDKTRSRILKAAI
Sbjct: 181 LDDGLVNEVRNLFDPK--GNDYARGIRRAIGVPELDAFLHAEMDSASDDKTRSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKENT KLACRQRQ IHRLQ+KW GW NLHRIDATEVFLQLGN +YS ++ WE LV E
Sbjct: 241 LKIKENTRKLACRQRQNIHRLQSKW-GW-NLHRIDATEVFLQLGNGEYS-NHVWETLVAE 300

Query: 301 PSSIIVDQFLSENSFKL-------TEKVFNAVF 327
           PSSII+DQFL ENSFK        T+KVFN VF
Sbjct: 301 PSSIIIDQFLCENSFKRHSVKIEHTDKVFNVVF 326

BLAST of PI0019164 vs. NCBI nr
Match: KAG6575015.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.2 bits (1341), Expect = 6.6e-144
Identity = 270/325 (83.08%), Postives = 288/325 (88.62%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQVA EPPLVNFQGT N LNL+PFFR+KDK+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSVSCKQVAAEPPLVNFQGT-NILNLKPFFRQKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL+ILTNKVTEKEC GVPHHLLGIADPNSNFT  DFRRHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NS IVGK RLP++AGGSNSFIESLV++E EFRYRYDCCFLWVDVSLP+L+SFVSDRVDRM
Sbjct: 121 NS-IVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           L+NGLVNEVRNLFDP    NDY RGIRRAIGVPELDAFLR EMDS  DDK RSRILKAAI
Sbjct: 181 LDNGLVNEVRNLFDPK--ENDYTRGIRRAIGVPELDAFLRAEMDSATDDKARSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKEN+IKLA RQRQKIHRLQ+KW GW NLHRIDATEVFLQ G  +YS D+AWEALV E
Sbjct: 241 LKIKENSIKLALRQRQKIHRLQSKW-GW-NLHRIDATEVFLQHGGGEYS-DHAWEALVAE 300

Query: 301 PSSIIVDQFLSENSFKLTEKVFNAV 326
           PSSIIVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAAKIPMAV 318

BLAST of PI0019164 vs. NCBI nr
Match: KAG7013588.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.8 bits (1335), Expect = 3.3e-143
Identity = 269/325 (82.77%), Postives = 287/325 (88.31%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MK SLS SCKQVA EPPLVNFQGT N LNL+PFFR+KDK+VFVMGATGTGKSRLAI+LA 
Sbjct: 1   MKFSLSVSCKQVAAEPPLVNFQGT-NILNLKPFFRQKDKLVFVMGATGTGKSRLAIELAN 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSAI 120
           RFPAEIINSDKIQ+YKGL+ILTNKVTEKEC GVPHHLLGIADPNSNFT  DFRRHAS AI
Sbjct: 61  RFPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAI 120

Query: 121 NSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRM 180
           NS IVGK RLP++AGGSNSFIESLV++E EFRYRYDCCFLWVDVSLP+L+SFVSDRVDRM
Sbjct: 121 NS-IVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRM 180

Query: 181 LENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAI 240
           L+NGLV EVRNLFDP    NDY RGIRRAIGVPELDAFLR EMDS  DDK RSRILKAAI
Sbjct: 181 LDNGLVKEVRNLFDPK--ENDYTRGIRRAIGVPELDAFLRAEMDSATDDKARSRILKAAI 240

Query: 241 LKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVE 300
           LKIKEN+IKLA RQRQKIHRLQ+KW GW NLHRIDATEVFLQ G  +YS D+AWEALV E
Sbjct: 241 LKIKENSIKLALRQRQKIHRLQSKW-GW-NLHRIDATEVFLQHGGGEYS-DHAWEALVAE 300

Query: 301 PSSIIVDQFLSENSFKLTEKVFNAV 326
           PSSIIVDQFL ENS  L  K+  AV
Sbjct: 301 PSSIIVDQFLCENSCSLAAKIPMAV 318

BLAST of PI0019164 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 349.0 bits (894), Expect = 4.2e-96
Identity = 188/306 (61.44%), Postives = 236/306 (77.12%), Query Frame = 0

Query: 7   TSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI 66
           T+ +QV ++P  +NFQG  N +++ PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI
Sbjct: 6   TALRQV-IQPLSLNFQG--NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEI 65

Query: 67  INSDKIQVYKGLKILTNKVTEKECRGVPHHLLG-IADPNSNFTVADFRRHASSAINSIIV 126
           +NSDKIQVYKGL I+TNKVT +E  GVPHHLLG + D   +FT  DF+R A  A+ S IV
Sbjct: 66  VNSDKIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVES-IV 125

Query: 127 GKRRLPVLAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGL 186
            + R+P++AGGSNS+IE+LV +  +FR RY+CCFLWVDVS P+L+SFVS+RVD+M++ GL
Sbjct: 126 QRDRVPIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGL 185

Query: 187 VNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKAAILKIKE 246
           V+EVR +FDP+   +DY  GIRRAIGVPELD FLR+EM      +T  R+L+ AI KIKE
Sbjct: 186 VDEVRRIFDPS--SSDYSAGIRRAIGVPELDEFLRSEM-RNYPAETTERLLETAIEKIKE 245

Query: 247 NTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVEPSSII 306
           NT  LACRQ QKI RL  +W+ W N+HR+DATEVFL+ G      D AW+  V  PS++ 
Sbjct: 246 NTCLLACRQLQKIQRLYKQWK-W-NMHRVDATEVFLRRGE---EADEAWDNSVAHPSALA 298

Query: 307 VDQFLS 312
           V++FLS
Sbjct: 306 VEKFLS 298

BLAST of PI0019164 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 322.0 bits (824), Expect = 5.5e-88
Identity = 177/316 (56.01%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60
           MK S+S S KQV    P++ F+   + +N+  F   K+KV+FVMGATG+GKSRLAIDLAT
Sbjct: 1   MKFSIS-SLKQV---QPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLAT 60

Query: 61  RFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNS-NFTVADFRRHASSA 120
           RF  EIINSDKIQ+YKGL +LTNKVT KECRGVPHHLLG+ D  + N T   + R AS A
Sbjct: 61  RFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQA 120

Query: 121 INSIIVGKRRLPVLAGGSNSFIESLVEEEPEFRY-RYDCCFLWVDVSLPILNSFVSDRVD 180
           I S +    +LP++AGGSNS+IE+LV     F    YDCCF+WVDVSLP+LNSFVS RVD
Sbjct: 121 I-SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVD 180

Query: 181 RMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRILKA 240
           RM+E GL+ EVR +F+P     +Y  GIRRAIGVPEL  +LR E  S +D  T+S++L  
Sbjct: 181 RMMEAGLLEEVREVFNPKA---NYSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDV 240

Query: 241 AILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALV 300
           A+  IK+NT  LACRQ +KI RL  KW+   ++HR+DATEVFL+    +   D AWE LV
Sbjct: 241 AVKNIKKNTEILACRQLKKIQRLHKKWK--MSMHRVDATEVFLKRNVEEQ--DEAWENLV 300

Query: 301 VEPSSIIVDQFLSENS 315
             PS  IVD+F + N+
Sbjct: 301 ARPSERIVDKFYNNNN 302

BLAST of PI0019164 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-79
Identity = 158/314 (50.32%), Postives = 221/314 (70.38%), Query Frame = 0

Query: 1   MKISLSTSCKQVAVEPPLVNFQGTN---NTLNLEPFFRRKDKVVFVMGATGTGKSRLAID 60
           MKIS++  CKQ     P ++F       N L L P +  KDKVV +MGATGTGKSRL++D
Sbjct: 3   MKISMA-MCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHAS 120
           +ATRF AEIINSDKIQV++GL I+TNK+T +E  GVPHHLLG+  P ++ T A++   A+
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 SAINSIIVGKRRLPVLAGGSNSFIESLVEE-EPEFRYRYDCCFLWVDVSLPILNSFVSDR 180
            +I S++  + +LP++ GGSNS++E+LV++ E +FR RYDCCFLWVDV+LP+L+ FVS+R
Sbjct: 123 LSIESVL-NRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSER 182

Query: 181 VDRMLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRTEMDSTIDDKTRSRIL 240
           VD+M+E+G+V EVR  FD +   +DY RGI++AIG PE D F R E    ++D  R  +L
Sbjct: 183 VDKMVESGMVEEVREFFDFS--NSDYSRGIKKAIGFPEFDRFFRNEQFLNVED--REELL 242

Query: 241 KAAILKIKENTIKLACRQRQKIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEA 300
              + +IK NT +LACRQR+KI RL+ K + W ++ R+DAT VF +  ++    + AWE 
Sbjct: 243 SKVLEEIKRNTFELACRQREKIERLR-KVKKW-SIQRVDATPVFTKRRSK-MDANVAWER 302

Query: 301 LVVEPSSIIVDQFL 311
           LV  PS+  V +FL
Sbjct: 303 LVAGPSTDTVSRFL 306

BLAST of PI0019164 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 227.3 bits (578), Expect = 1.8e-59
Identity = 128/299 (42.81%), Postives = 185/299 (61.87%), Query Frame = 0

Query: 36  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPH 95
           RKDKVV ++GATG GKSRL++DLATRFP+EIINSDKIQVY+GL+I TN++T ++ RGVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 96  HLLGIADP-NSNFTVADFRRHASSAINSIIVGKRRLPVLAGGSNSFIESLVEE--EPEF- 155
           HLLG+ +P +   T  +FR  AS+ +   I  ++++P++AGGSNSF+ +L+ +  +P+F 
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKE-ITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 182

Query: 156 -----------RYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGLVNEVRNLFDPNGLGN 215
                        RY+CCF+WVDVS  +L  ++  RVD M+++G+  E+   +DP   G 
Sbjct: 183 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 242

Query: 216 DYGRGIRRAIGVPELDAFLR---TEMDSTIDDKTRSRILKAAILKIKENTIKLACRQRQK 275
           +   GIR+AIGVPE D + +    E      D  R      A+  IK NT  LA RQ +K
Sbjct: 243 ETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKK 302

Query: 276 IHRLQNKWRGWNNLHRIDATEVF---LQLGNRDYSFDNAWEALVVEPSSIIVDQFLSEN 314
           I  L++   GW  + R+DAT  F   +   + +  +   WE  V+EPS  IV + L +N
Sbjct: 303 IEMLKD--AGW-EIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of PI0019164 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 202.6 bits (514), Expect = 4.8e-52
Identity = 122/294 (41.50%), Postives = 174/294 (59.18%), Query Frame = 0

Query: 37  KDKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLKILTNKVTEKECRGVPHH 96
           K KVV +MGATG+GKS L+IDLATRF  EI+NSDKIQ Y GLK+ TN+++  E  GVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 97  LLGIADP-NSNFTVADFRRHASSAINSIIVGKRRLPVLAGGSNSFIESLV---------- 156
           LLG   P +S  T ++FR  AS +I S I  +  LP++AGGSNSFI +L+          
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSI-SEITARGNLPIIAGGSNSFIHALLVDRFDPKTYP 161

Query: 157 ---EEEPEFRYRYDCCFLWVDVSLPILNSFVSDRVDRMLENGLVNEVRNLFDPNGLGNDY 216
              E       RY+CCFLWVDVS+ +L  ++S RVD+M+E+G+  E+   +DP   G+  
Sbjct: 162 FSSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAI 221

Query: 217 -GRGIRRAIGVPELDAFL-----RTEMDSTIDDKTRSRILKAAILKIKENTIKLACRQRQ 276
              GI + IG+PE D +        +   +  D+ R      A+ +IKENT +LA +Q +
Sbjct: 222 RAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIE 281

Query: 277 KIHRLQNKWRGWNNLHRIDATEVFLQLGNRDYSFDNAWEALVVEPSSIIVDQFL 311
           +I +L++   GW ++ R+DAT  F        S    W+  V++ S  +V +FL
Sbjct: 282 RIMKLKSS--GW-DIQRLDATPSF------GRSSREIWDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94ID25.9e-9561.44Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID17.7e-8756.01Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q93WC92.2e-7850.32Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q5GHF71.4e-5944.34Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID32.6e-5842.81Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A5A7STV92.3e-17494.21Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3C8452.3e-17494.21adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 G... [more]
A0A0A0KHK31.2e-17292.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1[more]
A0A6J1H3C21.9e-14181.85adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KZJ76.2e-14080.92adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_008458554.14.7e-17494.21PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo]... [more]
XP_004150320.12.6e-17292.94adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN47261.1 ... [more]
XP_038875913.17.3e-15184.38adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida][more]
KAG6575015.16.6e-14483.08Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper... [more]
KAG7013588.13.3e-14382.77Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT5G19040.14.2e-9661.44isopentenyltransferase 5 [more]
AT3G23630.15.5e-8856.01isopentenyltransferase 7 [more]
AT3G63110.11.6e-7950.32isopentenyltransferase 3 [more]
AT1G68460.11.8e-5942.81isopentenyltransferase 1 [more]
AT3G19160.14.8e-5241.50ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 37..310
e-value: 5.8E-105
score: 352.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 40..148
NoneNo IPR availablePFAMPF01715IPPTcoord: 73..148
e-value: 4.1E-21
score: 75.8
coord: 152..258
e-value: 5.9E-11
score: 42.5
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 166..271
e-value: 5.8E-105
score: 352.3
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 28..316
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 28..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0019164.1PI0019164.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity