PI0018935 (gene) Melon (PI 482460) v1

Overview


Name	PI0018935
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	PseudoU_synth_1 domain-containing protein
Location	chr12: 24613585 .. 24614590 (+)
RNA-Seq Expression	PI0018935
Synteny	PI0018935

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATCCCTGAAAGTGCATGGATGGGCGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAAGGTTTTGGCATTGTACCTTCACAGAGGTGAGTGTTCGAGGTTTTTTCATTGGTGCTTTTAAGTGAGTAAATGAAGTTTTATCACCAGCATGATACACTTTTACGCATAAACTACAAAGTGGTAACATTGCATCGATTTTGTTACTTGTCAAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTTCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAATTGATTCCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTGAGGTACGTTATGATCTCCTTCATTTAATTACATACGGATCGTATTGCCCCTTCCTTCACTGCAAGAAACATGATTTGTTAGTATTTGATATAGTATAGTGCTGTTATTTGGGAAGACAATTATATGGCGTTGAAGAACAAAGGTGGATTCTTTTTGGCCACAGGGGGAGCATCCTTTTCATAATTATACAATACGTGCCAATTATAGAACGAGAAGTCGAGCCAAGCAATCTCCAGGATATGCCCTTAAGTCAGGCAACAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAATCAAGGAACCATATCAACATTCTCCAAGCTTAAGCGTTTGTGAAAATCTAACTGAAGAATGCAGTAAACTTGAATATGGATCAGAACTTCTGAATGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGTGCCTCACACTTCAGAAGGATTCTTTGCTGTTCTTGTGAGAAGCTGGAAACATCTTTTGGATATGATTTAATTGAGATATCCATCTGGGGAGAATCGTTCATGTTGCATCAAGTTAGTCTTTCTCTAACATTGTTTTTATGA

mRNA sequence

ATGGAAATCCCTGAAAGTGCATGGATGGGCGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAAGGTTTTGGCATTGTACCTTCACAGAGGTGAGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTTCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAATTGATTCCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTGAGGGGGAGCATCCTTTTCATAATTATACAATACGTGCCAATTATAGAACGAGAAGTCGAGCCAAGCAATCTCCAGGATATGCCCTTAAGTCAGGCAACAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAATCAAGGAACCATATCAACATTCTCCAAGCTTAAGCGTTTGTGAAAATCTAACTGAAGAATGCAGTAAACTTGAATATGGATCAGAACTTCTGAATGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGTGCCTCACACTTCAGAAGGATTCTTTGCTGTTCTTGTGAGAAGCTGGAAACATCTTTTGGATATGATTTAATTGAGATATCCATCTGGGGAGAATCGTTCATGTTGCATCAAGTTAGTCTTTCTCTAACATTGTTTTTATGA

Coding sequence (CDS)

ATGGAAATCCCTGAAAGTGCATGGATGGGCGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAAGGTTTTGGCATTGTACCTTCACAGAGGTGAGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTTCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAATTGATTCCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTGAGGGGGAGCATCCTTTTCATAATTATACAATACGTGCCAATTATAGAACGAGAAGTCGAGCCAAGCAATCTCCAGGATATGCCCTTAAGTCAGGCAACAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAATCAAGGAACCATATCAACATTCTCCAAGCTTAAGCGTTTGTGAAAATCTAACTGAAGAATGCAGTAAACTTGAATATGGATCAGAACTTCTGAATGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGTGCCTCACACTTCAGAAGGATTCTTTGCTGTTCTTGTGAGAAGCTGGAAACATCTTTTGGATATGATTTAATTGAGATATCCATCTGGGGAGAATCGTTCATGTTGCATCAAGTTAGTCTTTCTCTAACATTGTTTTTATGA

Protein sequence

MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGNKVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVRARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQVSLSLTLFL

Homology

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BLAST of PI0018935 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.0e-48
Identity = 102/233 (43.78%), Postives = 143/233 (61.37%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPE+AW  DP G  LA  I+ +LP+NI+V ++ L     FDPR+EC +RKYSYLLP  
Sbjct: 139 MEIPETAWKDDPQGTVLAKCISKHLPENIRVFSV-LPSNRRFDPRRECTLRKYSYLLPVD 198

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           ++GI++   + EID HI+DF +IL  FEGE+PFHNYT R+ YR +S  K      +K  N
Sbjct: 199 VLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK------IKQRN 258

Query: 121 KVSPGDMSNSEFEESDGEDDTFVE-GIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGV 180
              P +  + +  ES+  ++  +E G +E  +     S    +T +    +  S      
Sbjct: 259 GRPPREPKSIKASESEFREENHIEIGEEEEEKEVDGESDEHVVTPDSDNSQVYS------ 318

Query: 181 RARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           RA+WL+EPDE D+I  +HFR++  C   KLE S G+  +EISIWGESFMLHQ+
Sbjct: 319 RAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGESFMLHQI 358

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BLAST of PI0018935 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.0e-115
Identity = 208/232 (89.66%), Postives = 214/232 (92.24%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLADYINSYLPDNIKV  + +    SFDPRKECDVRKYSYLLPAK
Sbjct: 148 MEIPESAWMGDPCGISLADYINSYLPDNIKVFGI-VPSQRSFDPRKECDVRKYSYLLPAK 207

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDILNSFEGEHPFHNYTIR+NYRTR RAKQSPGYALKSGN
Sbjct: 208 LIGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGN 267

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPG+MSN EFEESD EDDTFVEG+KEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 268 KVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVR 327

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           ARWLHEPDEKDRIGASHFRRILCCSC KLETSFGYD IEISIWGESFMLHQ+
Sbjct: 328 ARWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQI 378

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BLAST of PI0018935 vs. ExPASy TrEMBL
Match: A0A5A7V0I4 (Putative tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold340G00400 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.3e-115
Identity = 210/239 (87.87%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLADYINSYLPDNIKV  + +    SFDPRKECDVRKYSYLLPAK
Sbjct: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVFGI-VPSQRSFDPRKECDVRKYSYLLPAK 60

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDILNSFEGEHPFHNYTIR+NYRTR RAKQSPGYALKSGN
Sbjct: 61  LIGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGN 120

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPG+MSN EFEESD EDDTFVEG+KEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 121 KVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVR 180

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQVSLSLTLF 240
           ARWLHEPDEKDRIGASHFRRILCCSC KLETSFGYD IEISIWGESFMLHQ+   L  F
Sbjct: 181 ARWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQLVALLLRF 238

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BLAST of PI0018935 vs. ExPASy TrEMBL
Match: A0A5D3BFP0 (Putative tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00490 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.3e-115
Identity = 210/239 (87.87%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLADYINSYLPDNIKV  + +    SFDPRKECDVRKYSYLLPAK
Sbjct: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVFGI-VPSQRSFDPRKECDVRKYSYLLPAK 60

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDILNSFEGEHPFHNYTIR+NYRTR RAKQSPGYALKSGN
Sbjct: 61  LIGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGN 120

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPG+MSN EFEESD EDDTFVEG+KEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 121 KVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVR 180

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQVSLSLTLF 240
           ARWLHEPDEKDRIGASHFRRILCCSC KLETSFGYD IEISIWGESFMLHQ+   L  F
Sbjct: 181 ARWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQLVALLLRF 238

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BLAST of PI0018935 vs. ExPASy TrEMBL
Match: A0A0A0M0Z2 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533680 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.5e-103
Identity = 193/232 (83.19%), Postives = 202/232 (87.07%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIP SAW+ DPCGISLADYINSYLPDNIKV  + +   + FDPRKECDVR YSYLLPAK
Sbjct: 62  MEIPGSAWIDDPCGISLADYINSYLPDNIKVFGI-VPAQKRFDPRKECDVRMYSYLLPAK 121

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDIL SFEG+HPFHNYT+R+ YRTR RAKQ   YALKSG 
Sbjct: 122 LIGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGK 181

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPGDMSNSEFE SD E+DTFVEGIKEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 182 KVSPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVR 241

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           ARWLHEPDEKDRIGASHFRRIL CSC KLET FG D IEISIWGESFMLHQ+
Sbjct: 242 ARWLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQI 291

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BLAST of PI0018935 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.3e-98
Identity = 182/234 (77.78%), Postives = 199/234 (85.04%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIP+SAWMGDPCGI LA+YIN YLP+NIKV  + +    SFDPR+ECDVRKY+YLLPAK
Sbjct: 148 MEIPDSAWMGDPCGILLANYINFYLPENIKVFGI-VPSQRSFDPRRECDVRKYTYLLPAK 207

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKL TTEID HIS+FKDILNSF+GEHPFHNYTIR+ YRTR RA QSPGY  KSG+
Sbjct: 208 LIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRANQSPGYVHKSGD 267

Query: 121 KVSPGDMSNSEFEESDGEDDTFV-EGIKEPYQHSPSLSVC-ENLTEECSKLEYGSELLNG 180
            VS  D+SNSEFEESDGE+D FV  GIKEP QHSPSLS C EN  E CSKL  GSEL  G
Sbjct: 268 GVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHSPSLSGCNENTFEVCSKLGNGSELPQG 327

Query: 181 VRARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           VRARWLHEPDE+DRIGASHFR+ILCCSC KLETS GYD +EISIWGESFMLHQ+
Sbjct: 328 VRARWLHEPDERDRIGASHFRKILCCSCGKLETSLGYDFVEISIWGESFMLHQI 380

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BLAST of PI0018935 vs. NCBI nr
Match: XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 426.0 bits (1094), Expect = 2.1e-115
Identity = 208/232 (89.66%), Postives = 214/232 (92.24%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLADYINSYLPDNIKV  + +    SFDPRKECDVRKYSYLLPAK
Sbjct: 148 MEIPESAWMGDPCGISLADYINSYLPDNIKVFGI-VPSQRSFDPRKECDVRKYSYLLPAK 207

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDILNSFEGEHPFHNYTIR+NYRTR RAKQSPGYALKSGN
Sbjct: 208 LIGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGN 267

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPG+MSN EFEESD EDDTFVEG+KEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 268 KVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVR 327

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           ARWLHEPDEKDRIGASHFRRILCCSC KLETSFGYD IEISIWGESFMLHQ+
Sbjct: 328 ARWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQI 378

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BLAST of PI0018935 vs. NCBI nr
Match: TYJ97255.1 (putative tRNA pseudouridine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 425.6 bits (1093), Expect = 2.8e-115
Identity = 210/239 (87.87%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLADYINSYLPDNIKV  + +    SFDPRKECDVRKYSYLLPAK
Sbjct: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVFGI-VPSQRSFDPRKECDVRKYSYLLPAK 60

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDILNSFEGEHPFHNYTIR+NYRTR RAKQSPGYALKSGN
Sbjct: 61  LIGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGN 120

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPG+MSN EFEESD EDDTFVEG+KEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 121 KVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVR 180

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQVSLSLTLF 240
           ARWLHEPDEKDRIGASHFRRILCCSC KLETSFGYD IEISIWGESFMLHQ+   L  F
Sbjct: 181 ARWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQLVALLLRF 238

[Close]

BLAST of PI0018935 vs. NCBI nr
Match: KAA0059996.1 (putative tRNA pseudouridine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 425.6 bits (1093), Expect = 2.8e-115
Identity = 210/239 (87.87%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLADYINSYLPDNIKV  + +    SFDPRKECDVRKYSYLLPAK
Sbjct: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVFGI-VPSQRSFDPRKECDVRKYSYLLPAK 60

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDILNSFEGEHPFHNYTIR+NYRTR RAKQSPGYALKSGN
Sbjct: 61  LIGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGN 120

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPG+MSN EFEESD EDDTFVEG+KEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 121 KVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVR 180

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQVSLSLTLF 240
           ARWLHEPDEKDRIGASHFRRILCCSC KLETSFGYD IEISIWGESFMLHQ+   L  F
Sbjct: 181 ARWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQLVALLLRF 238

[Close]

BLAST of PI0018935 vs. NCBI nr
Match: XP_038894776.1 (putative tRNA pseudouridine synthase isoform X3 [Benincasa hispida])

HSP 1 Score: 392.1 bits (1006), Expect = 3.4e-105
Identity = 195/240 (81.25%), Postives = 211/240 (87.92%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPESAWMGDPCGISLA+YIN YLPDNIKV  + +   +SFDPRKECDVRKYSY+LPAK
Sbjct: 148 MEIPESAWMGDPCGISLANYINFYLPDNIKVFGI-VPSQKSFDPRKECDVRKYSYVLPAK 207

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGI +KLKTTE+D HISDFKDILNSF+GEHPFHNYTIR+ YRTR RAK+SPGY    G+
Sbjct: 208 LIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFHNYTIRSKYRTRIRAKRSPGY----GH 267

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVC-ENLTEECSKLEYGSELLNGV 180
           +VSP D+SNSEFEESDGEDDTFVEG+KEP Q SPSLSVC EN TE CSKL  GSEL   V
Sbjct: 268 EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSPSLSVCYENPTEVCSKLGDGSELPKDV 327

Query: 181 RARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQVSLSLTLF 240
           RARWLHEPDEKDRIGASHFR+ILCCSC KLETSFGYD +EISIWGESFMLHQVSLSLT F
Sbjct: 328 RARWLHEPDEKDRIGASHFRKILCCSCGKLETSFGYDFVEISIWGESFMLHQVSLSLTCF 382

[Close]

BLAST of PI0018935 vs. NCBI nr
Match: XP_031742713.1 (putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus])

HSP 1 Score: 384.0 bits (985), Expect = 9.3e-103
Identity = 193/232 (83.19%), Postives = 202/232 (87.07%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIP SAW+ DPCGISLADYINSYLPDNIKV  + +   + FDPRKECDVR YSYLLPAK
Sbjct: 167 MEIPGSAWIDDPCGISLADYINSYLPDNIKVFGI-VPAQKRFDPRKECDVRMYSYLLPAK 226

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           LIGIESKLKTTEID HISDFKDIL SFEG+HPFHNYT+R+ YRTR RAKQ   YALKSG 
Sbjct: 227 LIGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGK 286

Query: 121 KVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGVR 180
           KVSPGDMSNSEFE SD E+DTFVEGIKEP Q SPSLSVCENLTE CSKLE GSELL GVR
Sbjct: 287 KVSPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVR 346

Query: 181 ARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           ARWLHEPDEKDRIGASHFRRIL CSC KLET FG D IEISIWGESFMLHQ+
Sbjct: 347 ARWLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQI 396

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BLAST of PI0018935 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 193.4 bits (490), Expect = 2.2e-49
Identity = 102/233 (43.78%), Postives = 143/233 (61.37%), Query Frame = 0


Query: 1   MEIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAK 60
           MEIPE+AW  DP G  LA  I+ +LP+NI+V ++ L     FDPR+EC +RKYSYLLP  
Sbjct: 139 MEIPETAWKDDPQGTVLAKCISKHLPENIRVFSV-LPSNRRFDPRRECTLRKYSYLLPVD 198

Query: 61  LIGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALKSGN 120
           ++GI++   + EID HI+DF +IL  FEGE+PFHNYT R+ YR +S  K      +K  N
Sbjct: 199 VLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK------IKQRN 258

Query: 121 KVSPGDMSNSEFEESDGEDDTFVE-GIKEPYQHSPSLSVCENLTEECSKLEYGSELLNGV 180
              P +  + +  ES+  ++  +E G +E  +     S    +T +    +  S      
Sbjct: 259 GRPPREPKSIKASESEFREENHIEIGEEEEEKEVDGESDEHVVTPDSDNSQVYS------ 318

Query: 181 RARWLHEPDEKDRIGASHFRRILCCSCEKLETSFGYDLIEISIWGESFMLHQV 233
           RA+WL+EPDE D+I  +HFR++  C   KLE S G+  +EISIWGESFMLHQ+
Sbjct: 319 RAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGESFMLHQI 358

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BLAST of PI0018935 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 45.4 bits (106), Expect = 7.3e-05
Identity = 60/260 (23.08%), Postives = 100/260 (38.46%), Query Frame = 0


Query: 2   EIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAKL 61
           ++    ++ DP G    + +NS LP+ I++   Y H   SF  +K CD R+Y YLLP   
Sbjct: 89  QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFG-YKHVTPSFSSKKFCDRRRYVYLLPVFA 148

Query: 62  IGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALK---- 121
           +   S      + + +   ++ +  FE                R R K  PG   K    
Sbjct: 149 LDPISHRDRETVMASLGPGEEYVKCFECSE-------------RGR-KIPPGLVGKWKGT 208

Query: 122 -SGNKVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSV-CENLTEECSKLEYGSEL 181
             G K        S    S    D  +E +        S+ V    + E+  KL   S  
Sbjct: 209 NFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDSCKLNTNSSE 268

Query: 182 LNGVRARWLHEPDEKDR--------IGASHF---------------RRILCCSCEKLETS 233
              V++++ +   EK+R        +G+ +F               R+I+  +   +   
Sbjct: 269 TK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINL 328

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BLAST of PI0018935 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 45.4 bits (106), Expect = 7.3e-05
Identity = 60/260 (23.08%), Postives = 100/260 (38.46%), Query Frame = 0


Query: 2   EIPESAWMGDPCGISLADYINSYLPDNIKVLALYLHRGESFDPRKECDVRKYSYLLPAKL 61
           ++    ++ DP G    + +NS LP+ I++   Y H   SF  +K CD R+Y YLLP   
Sbjct: 89  QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFG-YKHVTPSFSSKKFCDRRRYVYLLPVFA 148

Query: 62  IGIESKLKTTEIDSHISDFKDILNSFEGEHPFHNYTIRANYRTRSRAKQSPGYALK---- 121
           +   S      + + +   ++ +  FE                R R K  PG   K    
Sbjct: 149 LDPISHRDRETVMASLGPGEEYVKCFECSE-------------RGR-KIPPGLVGKWKGT 208

Query: 122 -SGNKVSPGDMSNSEFEESDGEDDTFVEGIKEPYQHSPSLSV-CENLTEECSKLEYGSEL 181
             G K        S    S    D  +E +        S+ V    + E+  KL   S  
Sbjct: 209 NFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDSCKLNTNSSE 268

Query: 182 LNGVRARWLHEPDEKDR--------IGASHF---------------RRILCCSCEKLETS 233
              V++++ +   EK+R        +G+ +F               R+I+  +   +   
Sbjct: 269 TK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINL 328

The following BLAST results are available for this feature:

BLAST of PI0018935 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22928	3.0e-48	43.78	Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032...	[more]

BLAST of PI0018935 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C5W1	1.0e-115	89.66	putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=...	[more]
A0A5A7V0I4	1.3e-115	87.87	Putative tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5D3BFP0	1.3e-115	87.87	Putative tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A0A0M0Z2	4.5e-103	83.19	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G53...	[more]
A0A6J1I156	3.3e-98	77.78	putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569...	[more]

BLAST of PI0018935 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008457308.1	2.1e-115	89.66	PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo]	[more]
TYJ97255.1	2.8e-115	87.87	putative tRNA pseudouridine synthase [Cucumis melo var. makuwa]	[more]
KAA0059996.1	2.8e-115	87.87	putative tRNA pseudouridine synthase [Cucumis melo var. makuwa]	[more]
XP_038894776.1	3.4e-105	81.25	putative tRNA pseudouridine synthase isoform X3 [Benincasa hispida]	[more]
XP_031742713.1	9.3e-103	83.19	putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus]	[more]

BLAST of PI0018935 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30320.1	2.2e-49	43.78	Pseudouridine synthase family protein	[more]
AT1G76120.1	7.3e-05	23.08	Pseudouridine synthase family protein	[more]
AT1G76120.2	7.3e-05	23.08	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 49..234 e-value: 1.8E-12 score: 49.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..130
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..137
None	No IPR available	PANTHER	PTHR11142:SF21	TRNA PSEUDOURIDINE SYNTHASE	coord: 1..232
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 1..232
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 16..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0018935.1	PI0018935.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding