PI0018452 (gene) Melon (PI 482460) v1

Overview


Name	PI0018452
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Alpha-amylase
Location	chr07: 22452384 .. 22454723 (-)
RNA-Seq Expression	PI0018452
Synteny	PI0018452

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAGCGTGTCAAAATACGGACAGGAGTACTGTACTCAATATTTTTACTAGAATCACTTCATTTTGTTGGTTAAGATTAAATTTGTTTTCCACATTTTTAGTCATTTGTATTGGTCAATAAAAATTTAAATCATTTTTAGTCATTTTGTGGGAAGAATAGTCTGATTTGAAGTGTCCTATTAAATAAAGAGGAATATTTGGATGTAACCCAAAATCCACATATTTCTGTACATAATATAAATATTTTAGAAATTTGTAAGAGAAATTTGTCACATGAGATCATCAAAGAATTTTTCTTAAATCCCCCAATTAGGGTTCTACCGTGTGAATGTGCCTTACGAGCACGACTAGAGAACTTGCGTAGAAAACAACCTCTTGTTTCTCAAAGATTAGAGGCTATATTGTCAGACGACCCACATTCAATCAGCGAGTCTTACAAGGTCACATGATTAATTCTTTAATTTGGAACTTTCCTCTTACTTCTCGACTTAGAACTTCTGCCTGTCCACCATTCATTCATCAAACATCTTCCTTTTCTTCAACTCGTTCTCACTATCTTTACATCAGACGCAAATGGAAGCTTATAAAACTAAAATTCCCACTACCCATTTCCATAACAATGGCATTTCTAGCTTCTTTCTGTTTCCTCTACCTCTCTCTTTCCCTCTTCCCATCCCCTTCTTCTTCCACCTTACTGTTTCAGGTAACCAAATCCACTTGACTACTTTACTCTCCTTCATGGATACTCACTTCTAAAAGCTTGAAATTGATTGTAATGAAAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAGTTCATTAAACAACATAATTCCTGACCTTGTAAATGCTAAGGTTACCCATGTATGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGCAAGCTTTCTTTCTCTCTGTTCTTCAGCTCATTATCAATAACAATTATCTAAATTTCTGTAAACCCTCTTACAGGGTACTTACCTGGAAGACTTTATGATCTCAATGCATCCAAATATGGAACCCAGAATGAACTCAAGTCCTTAATTGCTGCCCTCCATGGAAAGGGAATCAAAGCCATTGCTGATATAGTGATCAACCACAGAACAGCAGAGAAACAAGATCAGAGAGGAATTTGGTGTATTTTTGAAGGAGGAACTCTAGATAACCGTCTTGATTGGGGCCCTTCCTTCATTTGTAGCGATGACACTGAATATTCCGATGGAAAGGGAAATCCAGACAGTGGAGAACCTTATCCACCAGCACCTGATATCGATCATCTCAATCCACGAGTTCAGAGAGAAATATCAGAGTGGATGAGATGGTTGAAAACAGAAATAGGTTTTGATGGATGGAGATTCGATTTCGTAAAAGGCTATGCACCAAGTATTACAAAGCTTTACACGGATCAAACCTCACCAGATTTTGCAGTTGGTGAGAAATGGGATGAACTTTCATATGGACAGGACGATAAACCCAATCAAAACCAAGACGCACATCGGAATTCTCTTGCAAATTGGGTTAGGGCTTCAGGTGATACAGTCACAGCGTTTGATTTCACAACGAAAGGGATCCTTCAAGCTGCCGTTCAAGGTGAGCTATGGAGGTTGAAGGATTCCAATGAGAAACCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTAACTTTCATCGACAACCACGATACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAAGGATACGCATATATTCTGACTCATCCTGGAACACCTTGCATTGTAATTCATTCAACAAAACAGTTCAGATTTTTGAATTTCGAGATTTGATTACCTGTAATTGACATACTGTTTGTTTATTTGATTGTTGCAGTTCTACGATCATTTCGCGGATTGGGGATTGAAGGAGGAGATAACGAAATTGAGTGAAATTAGGGCAAAGAATGGGATTGGGAAAACGAGCAGGGTGAATATTATGGCTTCTGAAGGGGATTTGTACGTTGCTGTTGTTGATGGGAAAATTATAATGAAGATCGGAGCAAAGACTAATGTTGGAAATTTGATTCCGTCGGATTATGAGCTTGCCACTTCGGGAATGGAGTATGCTGTTTGGGAGAAGAAGTGAATCTTTGAATATCTGCCATTGATGGAATAATTCGTCGTCCGTTTCTATTTTGAAGAAAGTTTCAAAATAATAATATTATATTAACTGAGGGTGTAATTGAAGGAGGGACAACTTGAAAGATTGATGATACTATAGCTAATTAAATATGCTTATTTTAAGAATCTTAATAAATTATGGTTGCAAATATAGTCATCCAAATTTGAA

mRNA sequence

ATAAGCGTGTCAAAATACGGACAGGAGTACTGTACTCAATATTTTTACTAGAATCACTTCATTTTGTTGGTTAAGATTAAATTTGTTTTCCACATTTTTAGTCATTTGTATTGGTCAATAAAAATTTAAATCATTTTTAGTCATTTTGTGGGAAGAATAGTCTGATTTGAAGTGTCCTATTAAATAAAGAGGAATATTTGGATGTAACCCAAAATCCACATATTTCTGTACATAATATAAATATTTTAGAAATTTGTAAGAGAAATTTGTCACATGAGATCATCAAAGAATTTTTCTTAAATCCCCCAATTAGGGTTCTACCGTGTGAATGTGCCTTACGAGCACGACTAGAGAACTTGCGTAGAAAACAACCTCTTGTTTCTCAAAGATTAGAGGCTATATTGTCAGACGACCCACATTCAATCAGCGAGTCTTACAAGGTCACATGATTAATTCTTTAATTTGGAACTTTCCTCTTACTTCTCGACTTAGAACTTCTGCCTGTCCACCATTCATTCATCAAACATCTTCCTTTTCTTCAACTCGTTCTCACTATCTTTACATCAGACGCAAATGGAAGCTTATAAAACTAAAATTCCCACTACCCATTTCCATAACAATGGCATTTCTAGCTTCTTTCTGTTTCCTCTACCTCTCTCTTTCCCTCTTCCCATCCCCTTCTTCTTCCACCTTACTGTTTCAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAGTTCATTAAACAACATAATTCCTGACCTTGTAAATGCTAAGGTTACCCATGTATGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGGTACTTACCTGGAAGACTTTATGATCTCAATGCATCCAAATATGGAACCCAGAATGAACTCAAGTCCTTAATTGCTGCCCTCCATGGAAAGGGAATCAAAGCCATTGCTGATATAGTGATCAACCACAGAACAGCAGAGAAACAAGATCAGAGAGGAATTTGGTGTATTTTTGAAGGAGGAACTCTAGATAACCGTCTTGATTGGGGCCCTTCCTTCATTTGTAGCGATGACACTGAATATTCCGATGGAAAGGGAAATCCAGACAGTGGAGAACCTTATCCACCAGCACCTGATATCGATCATCTCAATCCACGAGTTCAGAGAGAAATATCAGAGTGGATGAGATGGTTGAAAACAGAAATAGGTTTTGATGGATGGAGATTCGATTTCGTAAAAGGCTATGCACCAAGTATTACAAAGCTTTACACGGATCAAACCTCACCAGATTTTGCAGTTGGTGAGAAATGGGATGAACTTTCATATGGACAGGACGATAAACCCAATCAAAACCAAGACGCACATCGGAATTCTCTTGCAAATTGGGTTAGGGCTTCAGGTGATACAGTCACAGCGTTTGATTTCACAACGAAAGGGATCCTTCAAGCTGCCGTTCAAGGTGAGCTATGGAGGTTGAAGGATTCCAATGAGAAACCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTAACTTTCATCGACAACCACGATACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAAGGATACGCATATATTCTGACTCATCCTGGAACACCTTGCATTTTCTACGATCATTTCGCGGATTGGGGATTGAAGGAGGAGATAACGAAATTGAGTGAAATTAGGGCAAAGAATGGGATTGGGAAAACGAGCAGGGTGAATATTATGGCTTCTGAAGGGGATTTGTACGTTGCTGTTGTTGATGGGAAAATTATAATGAAGATCGGAGCAAAGACTAATGTTGGAAATTTGATTCCGTCGGATTATGAGCTTGCCACTTCGGGAATGGAGTATGCTGTTTGGGAGAAGAAGTGAATCTTTGAATATCTGCCATTGATGGAATAATTCGTCGTCCGTTTCTATTTTGAAGAAAGTTTCAAAATAATAATATTATATTAACTGAGGGTGTAATTGAAGGAGGGACAACTTGAAAGATTGATGATACTATAGCTAATTAAATATGCTTATTTTAAGAATCTTAATAAATTATGGTTGCAAATATAGTCATCCAAATTTGAA

Coding sequence (CDS)

ATGATTAATTCTTTAATTTGGAACTTTCCTCTTACTTCTCGACTTAGAACTTCTGCCTGTCCACCATTCATTCATCAAACATCTTCCTTTTCTTCAACTCGTTCTCACTATCTTTACATCAGACGCAAATGGAAGCTTATAAAACTAAAATTCCCACTACCCATTTCCATAACAATGGCATTTCTAGCTTCTTTCTGTTTCCTCTACCTCTCTCTTTCCCTCTTCCCATCCCCTTCTTCTTCCACCTTACTGTTTCAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAGTTCATTAAACAACATAATTCCTGACCTTGTAAATGCTAAGGTTACCCATGTATGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGGTACTTACCTGGAAGACTTTATGATCTCAATGCATCCAAATATGGAACCCAGAATGAACTCAAGTCCTTAATTGCTGCCCTCCATGGAAAGGGAATCAAAGCCATTGCTGATATAGTGATCAACCACAGAACAGCAGAGAAACAAGATCAGAGAGGAATTTGGTGTATTTTTGAAGGAGGAACTCTAGATAACCGTCTTGATTGGGGCCCTTCCTTCATTTGTAGCGATGACACTGAATATTCCGATGGAAAGGGAAATCCAGACAGTGGAGAACCTTATCCACCAGCACCTGATATCGATCATCTCAATCCACGAGTTCAGAGAGAAATATCAGAGTGGATGAGATGGTTGAAAACAGAAATAGGTTTTGATGGATGGAGATTCGATTTCGTAAAAGGCTATGCACCAAGTATTACAAAGCTTTACACGGATCAAACCTCACCAGATTTTGCAGTTGGTGAGAAATGGGATGAACTTTCATATGGACAGGACGATAAACCCAATCAAAACCAAGACGCACATCGGAATTCTCTTGCAAATTGGGTTAGGGCTTCAGGTGATACAGTCACAGCGTTTGATTTCACAACGAAAGGGATCCTTCAAGCTGCCGTTCAAGGTGAGCTATGGAGGTTGAAGGATTCCAATGAGAAACCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTAACTTTCATCGACAACCACGATACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAAGGATACGCATATATTCTGACTCATCCTGGAACACCTTGCATTTTCTACGATCATTTCGCGGATTGGGGATTGAAGGAGGAGATAACGAAATTGAGTGAAATTAGGGCAAAGAATGGGATTGGGAAAACGAGCAGGGTGAATATTATGGCTTCTGAAGGGGATTTGTACGTTGCTGTTGTTGATGGGAAAATTATAATGAAGATCGGAGCAAAGACTAATGTTGGAAATTTGATTCCGTCGGATTATGAGCTTGCCACTTCGGGAATGGAGTATGCTGTTTGGGAGAAGAAGTGA

Protein sequence

MINSLIWNFPLTSRLRTSACPPFIHQTSSFSSTRSHYLYIRRKWKLIKLKFPLPISITMAFLASFCFLYLSLSLFPSPSSSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWEKK

Homology

[Close]

BLAST of PI0018452 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 3.9e-196
Identity = 319/418 (76.32%), Postives = 358/418 (85.65%), Query Frame = 0


Query: 62  LASFCFLYLSLSLFPSPSSSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHVWLPP 121
           L+ FC   L +SL P  SS  LLFQGFNWESS KGGWY+SL N IPDL NA +THVWLPP
Sbjct: 7   LSIFC---LFISLLPLFSSPALLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPP 66

Query: 122 PSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQR 181
           PSQSVS +GYLPGRLYDL+ASKYG++NELKSLIAA H KGIK +ADIVINHRTAE++D R
Sbjct: 67  PSQSVSPEGYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGR 126

Query: 182 GIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREIS 241
           GI+CIFEGGT D+R DWGPSFIC DDT YSDG GN DSGE Y  APDIDHLNP+VQRE+S
Sbjct: 127 GIYCIFEGGTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELS 186

Query: 242 EWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQ 301
           EWM WLKTEIGFDGWRFDFVKGYAPSI+K+Y +QT PDFAVGEKWD +SYGQD KPN NQ
Sbjct: 187 EWMNWLKTEIGFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQ 246

Query: 302 DAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVT 361
           D+HR +L NWV ++G  +TAFDFTTKGILQAAVQGELWRL D N KPPG IG+KPE+AVT
Sbjct: 247 DSHRGALVNWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVT 306

Query: 362 FIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIRAK 421
           FIDNHDTGSTQR+WPFPSDKV+QGYAYILTHPGTP IFYDHF DWGLKE+I KLS IR +
Sbjct: 307 FIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLR 366

Query: 422 NGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWE 480
           NGI + S V IMASEGDLYVA +D KI++KIG K ++GNLIPS+  +ATSG +YAVWE
Sbjct: 367 NGINEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

[Close]

BLAST of PI0018452 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 1.7e-162
Identity = 270/404 (66.83%), Postives = 327/404 (80.94%), Query Frame = 0


Query: 80  SSTLLFQGFNWESSSK-GGWYSSLNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYD 139
           SSTLLFQ FNWES  K GG+Y+SL+N I D+ NA +TH+WLPPPSQSV+ +GYLPG+LYD
Sbjct: 24  SSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYD 83

Query: 140 LNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDW 199
           LN+SKYG++ ELKSLI AL+ KGIKA+ADIVINHRTAE++D +  +C FEGGT D+RLDW
Sbjct: 84  LNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDW 143

Query: 200 GPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRF 259
            PSF+C +D ++  G GN D+G  +  APDIDHLNPRVQ+E+SEWM WLKTEIGF GWRF
Sbjct: 144 DPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRF 203

Query: 260 DFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQDAHRNSLANWV-RASGD 319
           D+V+GYA SITKLY   TSPDFAVGEKWD++ YG D K + +Q+ HR+ L  W+  A G 
Sbjct: 204 DYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGG 263

Query: 320 TVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPF 379
            +TAFDFTTKGILQ+AV+GELWRLKDS  KPPG IGI P +AVTFIDNHD   T R W F
Sbjct: 264 VLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---TFRTWVF 323

Query: 380 PSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIRAKNGIGKTSRVNIMASEG 439
           PSDKV+ GY YILTHPGTPCIFY+H+ +WGLKE I+KL  IR KNGIG TS V I A+E 
Sbjct: 324 PSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEA 383

Query: 440 DLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWEKK 482
           DLY+A++D K+IMKIG K +VG L+PS++ LA SG+++AVWEKK
Sbjct: 384 DLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEKK 423

[Close]

BLAST of PI0018452 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 570.9 bits (1470), Expect = 1.4e-161
Identity = 258/421 (61.28%), Postives = 317/421 (75.30%), Query Frame = 0


Query: 63  ASFCFLYLSL--SLFPSPSSSTLLFQGFNWES-SSKGGWYSSLNNIIPDLVNAKVTHVWL 122
           +S C L L +  SL  +   + +LFQGFNWES   +GGWY+ L   + D+  A VTHVWL
Sbjct: 5   SSLCLLLLVVLCSLTCNSGQAQVLFQGFNWESWKQQGGWYNMLKGQVDDIAKAGVTHVWL 64

Query: 123 PPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQD 182
           PPPS SV+ QGY+PGRLYDL+ASKYGT  ELKSLIAA HGKG++ +AD+VINHR AEK+D
Sbjct: 65  PPPSHSVAPQGYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKD 124

Query: 183 QRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQRE 242
            RG++C+FEGGT D+RLDWGP  ICSDDT+YSDG G+ D+GE +  APDIDHLNPRVQRE
Sbjct: 125 ARGVYCVFEGGTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRE 184

Query: 243 ISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQ 302
           +++W+ WLK+++GFDGWR DF KGY+  I K+Y +   P F V E W+ LSY  D KP  
Sbjct: 185 LTDWLNWLKSDVGFDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGDGKPAA 244

Query: 303 NQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESA 362
           NQD  R  L NWV A G     FDFTTKG+LQA VQGELWRL+D N K  G IG  PE A
Sbjct: 245 NQDQGRQELVNWVNAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWLPEKA 304

Query: 363 VTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIR 422
           VTF+DNHDTGSTQ++WPFPSDKV+QGYAYILTHPG PCIFYDH  DW LK+EIT L+ IR
Sbjct: 305 VTFVDNHDTGSTQKLWPFPSDKVMQGYAYILTHPGVPCIFYDHMFDWNLKQEITALAAIR 364

Query: 423 AKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWE 481
            +NGI   S++ I+ ++ D YVAVVD K+++KIG + +VGN +PSD+     G +Y+VWE
Sbjct: 365 ERNGINAGSKLRIVVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTVHGKDYSVWE 424

[Close]

BLAST of PI0018452 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 569.3 bits (1466), Expect = 4.1e-161
Identity = 264/422 (62.56%), Postives = 319/422 (75.59%), Query Frame = 0


Query: 69  YLSLSLF-------PSPSSSTLLFQGFNWES-SSKGGWYSSLNNIIPDLVNAKVTHVWLP 128
           +LSLSLF        S +S  +LFQGFNWES    GGWY+ L   + D+  A +THVWLP
Sbjct: 5   HLSLSLFLVLLGLSASLASGQVLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGITHVWLP 64

Query: 129 PPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQ 188
           P SQSV+ QGY+PGRLYDL+ASKYG + +LKSLI ALHGKG+KAIADIVINHRTAE +D 
Sbjct: 65  PASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDG 124

Query: 189 RGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREI 248
           RGI+CIFEGGT D RLDWGP  IC DD  Y+DG GNPD+G  +  APDIDHLN RVQ+E+
Sbjct: 125 RGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKEL 184

Query: 249 SEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQN 308
            EW+ WLK +IGFDGWRFDF KGY+  + K+Y D++ P FAV E W  L+YG D KPN N
Sbjct: 185 VEWLNWLKADIGFDGWRFDFAKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLN 244

Query: 309 QDAHRNSLANWVRASG--DTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPES 368
           QD HR  L NWV   G     T FDFTTKGIL  AV+GELWRL+ ++ K PG IG  P  
Sbjct: 245 QDQHRQELVNWVDKVGGKGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAK 304

Query: 369 AVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEI 428
           AVTF+DNHDTGSTQ MWPFPSD+V+QGYAYILTHPGTPCIFYDHF DWGLKEEI +L  +
Sbjct: 305 AVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSV 364

Query: 429 RAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVW 481
           R ++GI   S++ I+ ++ DLY+A +DGK+I+K+G + +VGNLIP  +++A  G +YAVW
Sbjct: 365 RTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVW 424

[Close]

BLAST of PI0018452 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 2.7e-160
Identity = 251/417 (60.19%), Postives = 317/417 (76.02%), Query Frame = 0


Query: 66  CFLYLSLSLFPSPSSSTLLFQGFNWESSSK-GGWYSSLNNIIPDLVNAKVTHVWLPPPSQ 125
           C ++  L L  S + + +LFQGFNWES  K GGWY+ L+  + ++ +   THVWLPPPS 
Sbjct: 10  CVVFAVLCLASSLAQAQVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSH 69

Query: 126 SVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQRGIW 185
           SVS QGY+PGRLYDL+ASKYGT+ ELKSLI A H K ++ +ADIVINHR A+ +D RG++
Sbjct: 70  SVSPQGYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVY 129

Query: 186 CIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREISEWM 245
           C+FEGGT D RLDWGP  ICSDDT+YS+G+G+ D+G  +  APDIDHLNPRVQRE+++W+
Sbjct: 130 CVFEGGTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWL 189

Query: 246 RWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQDAH 305
            WL+T++GFDGWR DF KGY+  + ++Y D T+P F VGE W  L Y  D KP+ NQDA 
Sbjct: 190 NWLRTDLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDAD 249

Query: 306 RNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVTFID 365
           R  L NWV   G   TAFDFTTKGILQAAVQGELWRL D N K PG +G  P+ AVTF+D
Sbjct: 250 RQELVNWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVD 309

Query: 366 NHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIRAKNGI 425
           NHDTGSTQ +WPFPSDKV+QGYAYILTHPG PCIFYDH  DW L+ EI  L+EIR++NGI
Sbjct: 310 NHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGI 369

Query: 426 GKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWEKK 482
              S ++I+ +EGD+YVA++DGK+I K+G + + G +IPSD+ +   G +Y VWEK+
Sbjct: 370 HAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEKE 426

[Close]

BLAST of PI0018452 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.7e-232
Identity = 384/436 (88.07%), Postives = 409/436 (93.81%), Query Frame = 0


Query: 46  LIKLKFPLPISITMAFLASFCFLYLSLSLFPSPSSSTLLFQGFNWESSSKGGWYSSLNNI 105
           LIK K+ LPIS TM FLAS CFLYLS+SLFPSPSSS LLFQGFNWESSSKGGWY+SL NI
Sbjct: 3   LIKAKYALPISKTMGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLKNI 62

Query: 106 IPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAI 165
           IPDLVNA++THVWLPPPSQSVS QGYLPGRLYDL+ASKYGTQNELKSLIAALH KGIKAI
Sbjct: 63  IPDLVNARITHVWLPPPSQSVSPQGYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAI 122

Query: 166 ADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPP 225
           ADIVINHRTAEKQDQRGIWCIFEGGT DNRLDWGPSFICSDDT+YSDGKGNPDSGEPYPP
Sbjct: 123 ADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPP 182

Query: 226 APDIDHLNPRVQREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEK 285
           APDIDHL P+VQ+EISEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLY DQTSPDFAVGEK
Sbjct: 183 APDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEK 242

Query: 286 WDELSYGQDDKPNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSN 345
           WD LS G D K NQNQDAHR+SLANWV+ASGD VTAFDFTTKGILQAAVQGELWRLKDSN
Sbjct: 243 WDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSN 302

Query: 346 EKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFAD 405
            KPPGFIGIKPESAVTF+DNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYDHF +
Sbjct: 303 GKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVE 362

Query: 406 WGLKEEITKLSEIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSD 465
           WGLKEEI +LSEIRA+N IGKTS VNI+AS+ DLYVA VDGKIIMKIGA TN+GNLIPS+
Sbjct: 363 WGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSE 422

Query: 466 YELATSGMEYAVWEKK 482
           ++LATSGMEYAVWEK+
Sbjct: 423 FQLATSGMEYAVWEKR 438

[Close]

BLAST of PI0018452 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.9e-224
Identity = 377/440 (85.68%), Postives = 400/440 (90.91%), Query Frame = 0


Query: 45  KLIKLKFPLPIS-ITMAFLASFCFL-YLSLSLFPSPS-SSTLLFQGFNWESSSKGGWYSS 104
           KLI  +FP  IS  TM FLAS CFL YLS+ LFPSPS SSTLLFQGFNWESSSKGGWY+S
Sbjct: 10  KLINAEFPQHISPNTMGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNS 69

Query: 105 LNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKG 164
           LNNIIPDLV+AKVTHVWLPPPSQS S QGYLPGRLYDLNASKYGTQNELKSLIAALH  G
Sbjct: 70  LNNIIPDLVHAKVTHVWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNG 129

Query: 165 IKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGE 224
           IKA+ADIVINHRTA+KQD+RGIWCIFEGGT D+RLDWGPSFICSDDTEYSDGKGNPDSGE
Sbjct: 130 IKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGE 189

Query: 225 PYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFA 284
           PY  APDIDHLNP+VQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLY DQTSPDFA
Sbjct: 190 PYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFA 249

Query: 285 VGEKWDELSYGQDDKPNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRL 344
           VGEKWD LS G D KP+ NQD HR +LANWVR SGDTVTAFDFTTKGILQAAVQGELWRL
Sbjct: 250 VGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRL 309

Query: 345 KDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYD 404
           KDSN KPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYD
Sbjct: 310 KDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYD 369

Query: 405 HFADWGLKEEITKLSEIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNL 464
           HF +WGLKEE+ KLS IRA+NGIG+ S VNIMAS+GDLYVA +DGKIIMKIG  TN+GNL
Sbjct: 370 HFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNL 429

Query: 465 IPSDYELATSGMEYAVWEKK 482
           +PSDYELA SGM+YAVWEKK
Sbjct: 430 VPSDYELAASGMQYAVWEKK 449

[Close]

BLAST of PI0018452 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.8e-220
Identity = 367/425 (86.35%), Postives = 390/425 (91.76%), Query Frame = 0


Query: 59  MAFLASFCFL-YLSLSLFPSPS-SSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTH 118
           M FLAS CFL YLS+ +FPSPS SSTLLFQGFNWESSSKGGWY+SLNNIIPDLV+AKVTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 119 VWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAE 178
           VWLPPPSQS S QGYLPGRLYDLNASKYGTQ+ELKSLIAALH  GIKA+ADIVINHRTA+
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 179 KQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRV 238
           KQD+RGIWCIFEGGT D+RLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNP+V
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 239 QREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDK 298
           Q E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLY DQTSPDFAVGEKWD LS G D K
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 299 PNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKP 358
           P+ NQD HR +LANWVR SGDTVTAFDFTTKGILQAAVQGELWRLKD N KPPGFIGIKP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 359 ESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLS 418
           ESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYDHF +WGLKEE+ KLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 419 EIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYA 478
            IR +NGIG+ S VNIMAS+GDLYVA +DGKIIMKIG  TN+GNLIPSDYELATSGM+YA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 479 VWEKK 482
           VWEKK
Sbjct: 421 VWEKK 425

[Close]

BLAST of PI0018452 vs. ExPASy TrEMBL
Match: A0A1S3V9G5 (Alpha-amylase OS=Vigna radiata var. radiata OX=3916 GN=LOC106772881 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 1.1e-193
Identity = 319/418 (76.32%), Postives = 359/418 (85.89%), Query Frame = 0


Query: 62  LASFCFLYLSLSLFPSPSSSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHVWLPP 121
           L+ FC   L +SL P  SS  LLFQGFNWESS KGGWY+SL N IPDL NA +THVWLPP
Sbjct: 7   LSIFC---LFISLLPLFSSPALLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPP 66

Query: 122 PSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQR 181
           PSQSVS +GYLPGRLYDL+AS+YG++NELKSLIAA H KGIK +ADIVINHRTAE++D R
Sbjct: 67  PSQSVSPEGYLPGRLYDLDASRYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGR 126

Query: 182 GIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREIS 241
           GI+CIFEGGT D+RLDWGPSFIC DDT YSDG GN DSGE Y  APDIDHLNP+VQRE+S
Sbjct: 127 GIYCIFEGGTPDSRLDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELS 186

Query: 242 EWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQ 301
           EWM WLKTEIGFDGWRFDFVKGYAPSI+K+Y +QT PDFAVGEKWD +SYGQD KPN NQ
Sbjct: 187 EWMNWLKTEIGFDGWRFDFVKGYAPSISKIYMEQTRPDFAVGEKWDAISYGQDGKPNYNQ 246

Query: 302 DAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVT 361
           D+HR +L NWV ++G  +TAFDFTTKGILQAAVQGELWRL D N KPPG IG+KPE+AVT
Sbjct: 247 DSHRGALVNWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVT 306

Query: 362 FIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIRAK 421
           FIDNHDTGSTQR+WPFPSDKV+QGYAYILTHPGTP IFYDHF DWGLKE+I KLS IR +
Sbjct: 307 FIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLR 366

Query: 422 NGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWE 480
           NGI + S V IMASEGDLYVA +D KI++KIG K ++GNLIPS+  +ATSG +YAVWE
Sbjct: 367 NGINEKSSVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

[Close]

BLAST of PI0018452 vs. ExPASy TrEMBL
Match: A0A2N9FNN7 (Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.4e-193
Identity = 316/423 (74.70%), Postives = 363/423 (85.82%), Query Frame = 0


Query: 59  MAFLASFCFLYLSLSLFPSPSSSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHVW 118
           M  L S CFL L +SL PS +   +LFQGFNWESS+KGGWY+ L N IPDL NA +THVW
Sbjct: 1   MNILTSLCFLCLCISLSPSFAVPAILFQGFNWESSNKGGWYNLLKNSIPDLANAGITHVW 60

Query: 119 LPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQ 178
           LPPPSQSV+ QGY+PGRLYDL+ASKYG+++ELKSLIAALH KGIKA+ADIVINHRTAEKQ
Sbjct: 61  LPPPSQSVAPQGYMPGRLYDLDASKYGSKDELKSLIAALHEKGIKALADIVINHRTAEKQ 120

Query: 179 DQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQR 238
           D RGIWCIFEGGT D RLDWGPSFIC DDT YSDG GNPDSG+ +  APDIDH+NP+VQ+
Sbjct: 121 DGRGIWCIFEGGTPDGRLDWGPSFICKDDTAYSDGTGNPDSGKDFGGAPDIDHVNPQVQK 180

Query: 239 EISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPN 298
           E+SEWM WLKTEIGFDGWRFDFV GYA S TK+Y +QT PDFAVGE W+ LSYG   KPN
Sbjct: 181 ELSEWMNWLKTEIGFDGWRFDFVLGYASSFTKVYMEQTKPDFAVGENWNSLSYGDGGKPN 240

Query: 299 QNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPES 358
            NQDAHRN+L +W++A+G  VTAFDFTTKGILQAAV+GELWRLKDSN KPPG IGIKPE+
Sbjct: 241 PNQDAHRNALVDWIQAAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPEN 300

Query: 359 AVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEI 418
           AVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYDH  DWGLK+EI+KL+ I
Sbjct: 301 AVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHLFDWGLKDEISKLTAI 360

Query: 419 RAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVW 478
           R++NGI     VNI+AS+ DLYVA +D KIIMKIG K ++GNLIPS+++++ SG +YAVW
Sbjct: 361 RSRNGINTQGIVNILASDADLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSASGKDYAVW 420

Query: 479 EKK 482
           EKK
Sbjct: 421 EKK 423

[Close]

BLAST of PI0018452 vs. NCBI nr
Match: XP_022142476.1 (alpha-amylase [Momordica charantia])

HSP 1 Score: 814.3 bits (2102), Expect = 5.6e-232
Identity = 384/436 (88.07%), Postives = 409/436 (93.81%), Query Frame = 0


Query: 46  LIKLKFPLPISITMAFLASFCFLYLSLSLFPSPSSSTLLFQGFNWESSSKGGWYSSLNNI 105
           LIK K+ LPIS TM FLAS CFLYLS+SLFPSPSSS LLFQGFNWESSSKGGWY+SL NI
Sbjct: 3   LIKAKYALPISKTMGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLKNI 62

Query: 106 IPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAI 165
           IPDLVNA++THVWLPPPSQSVS QGYLPGRLYDL+ASKYGTQNELKSLIAALH KGIKAI
Sbjct: 63  IPDLVNARITHVWLPPPSQSVSPQGYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAI 122

Query: 166 ADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPP 225
           ADIVINHRTAEKQDQRGIWCIFEGGT DNRLDWGPSFICSDDT+YSDGKGNPDSGEPYPP
Sbjct: 123 ADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPP 182

Query: 226 APDIDHLNPRVQREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEK 285
           APDIDHL P+VQ+EISEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLY DQTSPDFAVGEK
Sbjct: 183 APDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEK 242

Query: 286 WDELSYGQDDKPNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSN 345
           WD LS G D K NQNQDAHR+SLANWV+ASGD VTAFDFTTKGILQAAVQGELWRLKDSN
Sbjct: 243 WDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSN 302

Query: 346 EKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFAD 405
            KPPGFIGIKPESAVTF+DNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYDHF +
Sbjct: 303 GKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVE 362

Query: 406 WGLKEEITKLSEIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSD 465
           WGLKEEI +LSEIRA+N IGKTS VNI+AS+ DLYVA VDGKIIMKIGA TN+GNLIPS+
Sbjct: 363 WGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSE 422

Query: 466 YELATSGMEYAVWEKK 482
           ++LATSGMEYAVWEK+
Sbjct: 423 FQLATSGMEYAVWEKR 438

[Close]

BLAST of PI0018452 vs. NCBI nr
Match: XP_038896126.1 (alpha-amylase-like [Benincasa hispida])

HSP 1 Score: 807.0 bits (2083), Expect = 8.9e-230
Identity = 382/424 (90.09%), Postives = 403/424 (95.05%), Query Frame = 0


Query: 59  MAFLASFCFLYLSLSLFPSP-SSSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHV 118
           M FLAS CFLYL +SLFPSP SSSTLLFQGFNWESSSKGGWY+SLNNIIPDL++A+VTHV
Sbjct: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60

Query: 119 WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEK 178
           WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIA LH KGIKAIADIVINHRTAEK
Sbjct: 61  WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEK 120

Query: 179 QDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQ 238
           QDQRGIWCIFEGGT D+RLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNP+VQ
Sbjct: 121 QDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQ 180

Query: 239 REISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKP 298
           +E+SEWMRWLKTEIGFDGWRFDFVKGYAPSITKLY DQTSP+FAVGEKWD LSYGQD KP
Sbjct: 181 KELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKP 240

Query: 299 NQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPE 358
           NQNQD+HR++LANWVRASGD VTAFDFTTKGILQAAV+GELWRLKDSN  PPGFIGIKPE
Sbjct: 241 NQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPE 300

Query: 359 SAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSE 418
           SAVTFI+NHDTGSTQRMWPFPSDKVIQGYAYILTHPGTP IFYDHF DWGLKEEI KLS 
Sbjct: 301 SAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSG 360

Query: 419 IRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAV 478
           IRA+NGIGKTS VNIMASEGDLYVA VDGKIIMKIGA T++GNLIPSDY+LAT GM+YAV
Sbjct: 361 IRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAV 420

Query: 479 WEKK 482
           WEK+
Sbjct: 421 WEKR 424

[Close]

BLAST of PI0018452 vs. NCBI nr
Match: XP_022936258.1 (alpha-amylase, partial [Cucurbita moschata])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 377/440 (85.68%), Postives = 400/440 (90.91%), Query Frame = 0


Query: 45  KLIKLKFPLPIS-ITMAFLASFCFL-YLSLSLFPSPS-SSTLLFQGFNWESSSKGGWYSS 104
           KLI  +FP  IS  TM FLAS CFL YLS+ LFPSPS SSTLLFQGFNWESSSKGGWY+S
Sbjct: 10  KLINAEFPQHISPNTMGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNS 69

Query: 105 LNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKG 164
           LNNIIPDLV+AKVTHVWLPPPSQS S QGYLPGRLYDLNASKYGTQNELKSLIAALH  G
Sbjct: 70  LNNIIPDLVHAKVTHVWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNG 129

Query: 165 IKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGE 224
           IKA+ADIVINHRTA+KQD+RGIWCIFEGGT D+RLDWGPSFICSDDTEYSDGKGNPDSGE
Sbjct: 130 IKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGE 189

Query: 225 PYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFA 284
           PY  APDIDHLNP+VQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLY DQTSPDFA
Sbjct: 190 PYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFA 249

Query: 285 VGEKWDELSYGQDDKPNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRL 344
           VGEKWD LS G D KP+ NQD HR +LANWVR SGDTVTAFDFTTKGILQAAVQGELWRL
Sbjct: 250 VGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRL 309

Query: 345 KDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYD 404
           KDSN KPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYD
Sbjct: 310 KDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYD 369

Query: 405 HFADWGLKEEITKLSEIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNL 464
           HF +WGLKEE+ KLS IRA+NGIG+ S VNIMAS+GDLYVA +DGKIIMKIG  TN+GNL
Sbjct: 370 HFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNL 429

Query: 465 IPSDYELATSGMEYAVWEKK 482
           +PSDYELA SGM+YAVWEKK
Sbjct: 430 VPSDYELAASGMQYAVWEKK 449

[Close]

BLAST of PI0018452 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 776.9 bits (2005), Expect = 9.9e-221
Identity = 368/426 (86.38%), Postives = 391/426 (91.78%), Query Frame = 0


Query: 58  TMAFLASFCFL-YLSLSLFPSPS-SSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVT 117
           TM FLAS CFL YLS+ +FPSPS SSTLLFQGFNWESSSKGGWY+SLNNIIPDLV+AKVT
Sbjct: 42  TMGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVT 101

Query: 118 HVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTA 177
           HVWLPPPSQS S QGYLPGRLYDLNASKYGTQ+ELKSLIAALH  GIKA+ADIVINHRTA
Sbjct: 102 HVWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTA 161

Query: 178 EKQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPR 237
           +KQD+RGIWCIFEGGT D+RLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNP+
Sbjct: 162 DKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQ 221

Query: 238 VQREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDD 297
           VQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLY DQTSPDFAVGEKWD LS G D 
Sbjct: 222 VQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDG 281

Query: 298 KPNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIK 357
           KP+ NQD HR +LANWVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSN KPPGFIGIK
Sbjct: 282 KPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIK 341

Query: 358 PESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKL 417
           PESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYDHF +WGLKEE+ KL
Sbjct: 342 PESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKL 401

Query: 418 SEIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEY 477
           S IR +NGIG+ S VNIMAS+GDLYVA +DGKIIMKIG  TN+GNLIPSDY LATSGM+Y
Sbjct: 402 SAIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYVLATSGMQY 461

Query: 478 AVWEKK 482
           AVWEKK
Sbjct: 462 AVWEKK 467

[Close]

BLAST of PI0018452 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 775.0 bits (2000), Expect = 3.7e-220
Identity = 367/425 (86.35%), Postives = 390/425 (91.76%), Query Frame = 0


Query: 59  MAFLASFCFL-YLSLSLFPSPS-SSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTH 118
           M FLAS CFL YLS+ +FPSPS SSTLLFQGFNWESSSKGGWY+SLNNIIPDLV+AKVTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 119 VWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAE 178
           VWLPPPSQS S QGYLPGRLYDLNASKYGTQ+ELKSLIAALH  GIKA+ADIVINHRTA+
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 179 KQDQRGIWCIFEGGTLDNRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRV 238
           KQD+RGIWCIFEGGT D+RLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNP+V
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 239 QREISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDK 298
           Q E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLY DQTSPDFAVGEKWD LS G D K
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 299 PNQNQDAHRNSLANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKP 358
           P+ NQD HR +LANWVR SGDTVTAFDFTTKGILQAAVQGELWRLKD N KPPGFIGIKP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 359 ESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLS 418
           ESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP IFYDHF +WGLKEE+ KLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 419 EIRAKNGIGKTSRVNIMASEGDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYA 478
            IR +NGIG+ S VNIMAS+GDLYVA +DGKIIMKIG  TN+GNLIPSDYELATSGM+YA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 479 VWEKK 482
           VWEKK
Sbjct: 421 VWEKK 425

[Close]

BLAST of PI0018452 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-163
Identity = 270/404 (66.83%), Postives = 327/404 (80.94%), Query Frame = 0


Query: 80  SSTLLFQGFNWESSSK-GGWYSSLNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYD 139
           SSTLLFQ FNWES  K GG+Y+SL+N I D+ NA +TH+WLPPPSQSV+ +GYLPG+LYD
Sbjct: 24  SSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYD 83

Query: 140 LNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDW 199
           LN+SKYG++ ELKSLI AL+ KGIKA+ADIVINHRTAE++D +  +C FEGGT D+RLDW
Sbjct: 84  LNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDW 143

Query: 200 GPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRF 259
            PSF+C +D ++  G GN D+G  +  APDIDHLNPRVQ+E+SEWM WLKTEIGF GWRF
Sbjct: 144 DPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRF 203

Query: 260 DFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQDAHRNSLANWV-RASGD 319
           D+V+GYA SITKLY   TSPDFAVGEKWD++ YG D K + +Q+ HR+ L  W+  A G 
Sbjct: 204 DYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGG 263

Query: 320 TVTAFDFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPF 379
            +TAFDFTTKGILQ+AV+GELWRLKDS  KPPG IGI P +AVTFIDNHD   T R W F
Sbjct: 264 VLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---TFRTWVF 323

Query: 380 PSDKVIQGYAYILTHPGTPCIFYDHFADWGLKEEITKLSEIRAKNGIGKTSRVNIMASEG 439
           PSDKV+ GY YILTHPGTPCIFY+H+ +WGLKE I+KL  IR KNGIG TS V I A+E 
Sbjct: 324 PSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEA 383

Query: 440 DLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWEKK 482
           DLY+A++D K+IMKIG K +VG L+PS++ LA SG+++AVWEKK
Sbjct: 384 DLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEKK 423

[Close]

BLAST of PI0018452 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 371.7 bits (953), Expect = 8.9e-103
Identity = 186/403 (46.15%), Postives = 248/403 (61.54%), Query Frame = 0


Query: 79  SSSTLLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYD 138
           S   +L QGFNWES+  G WY  L     +L +   T +WLPPP++SVS +GY+P  LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 139 LNASKYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDW 198
           LN S+YGT +ELK  +   H  GIK + D V+NHR A  ++Q G+W +F G     RL+W
Sbjct: 553 LN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNW 612

Query: 199 GPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRF 258
               + +DD  +  G+GN  SG+ +  AP+IDH    V+++I EW+ W+  E+G+DGWR 
Sbjct: 613 DDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 672

Query: 259 DFVKGYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQDAHRNSLANWVRASGDT 318
           DFV+G+     K Y D + P FAVGE WD LSY   +  + NQDAHR  + +W+ A+   
Sbjct: 673 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEM-DYNQDAHRQRIVDWINATSGA 732

Query: 319 VTAFDFTTKGILQAAVQG-ELWRLKDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPF 378
             AFD TTKGIL  A+Q  E WRL D   KPPG +G  P  AVTFI+NHDTGSTQ  W F
Sbjct: 733 AGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRF 792

Query: 379 PSDKVIQGYAYILTHPGTPCIFYDH-FADWGLKEEITKLSEIRAKNGIGKTSRVNIMASE 438
           P  K +QGYAYILTHPGTP +F+DH F+D+    EI  L  +R +  +   S VNI  SE
Sbjct: 793 PEGKEMQGYAYILTHPGTPAVFFDHIFSDY--HSEIAALLSLRNRQKLHCRSEVNIDKSE 852

Query: 439 GDLYVAVVDGKIIMKIGAKTNVGNLIPSDYELATSGMEYAVWE 480
            D+Y A++D K+ MKIG           ++ +A  G +Y VWE
Sbjct: 853 RDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of PI0018452 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 365.9 bits (938), Expect = 4.9e-101
Identity = 182/402 (45.27%), Postives = 254/402 (63.18%), Query Frame = 0


Query: 83  LLFQGFNWESSSKGGWYSSLNNIIPDLVNAKVTHVWLPPPSQSVSSQGYLPGRLYDLNAS 142
           ++ Q +NWE S K  W+ +L+  +PD+  +  T  WLPPPSQS++ +GYLP  LY LN S
Sbjct: 27  VILQAYNWE-SHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-S 86

Query: 143 KYGTQNELKSLIAALHGKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTLDNRLDWGPSF 202
            YG+++ LKSL+  +    ++A+ADIVINHR    +   G++  ++G +    L W    
Sbjct: 87  AYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGIS----LPWDEHA 146

Query: 203 ICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPRVQREISEWMRWLKTEIGFDGWRFDFVK 262
           +    T  + G GN  +G+ +   P++DH    V+++I  W+RWL+  +GF  +RFDF +
Sbjct: 147 V----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFAR 206

Query: 263 GYAPSITKLYTDQTSPDFAVGEKWDELSYGQDDKPNQNQDAHRNSLANWVRASGDTVTAF 322
           GY+ +  K Y     P F+VGE WD  +Y      + NQD+HR  + +W+ A+G    AF
Sbjct: 207 GYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGL-DYNQDSHRQRIISWIDATGQISAAF 266

Query: 323 DFTTKGILQAAVQGELWRLKDSNEKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKV 382
           DFTTKGILQ AV+G+ WRL D+  KPPG +G  P  AVTF+DNHDTGSTQ  WPFPS  V
Sbjct: 267 DFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHV 326

Query: 383 IQGYAYILTHPGTPCIFYDHFADWG--LKEEITKLSEIRAKNGIGKTSRVNIMASEGDLY 442
           ++GYAYILTHPG PC+FYDHF DWG  + ++I KL +IR +  I   S V ++ +E +LY
Sbjct: 327 MEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLY 386

Query: 443 VAVVDGKIIMKIGAKTNVGNLIPS--DYELATSGMEYAVWEK 481
            A+V  KI MK+G     G+  PS  D+ LATSG  YAVW K
Sbjct: 387 AAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413

The following BLAST results are available for this feature:

BLAST of PI0018452 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17859	3.9e-196	76.32	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
Q8VZ56	1.7e-162	66.83	Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1	[more]
P27933	1.4e-161	61.28	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2...	[more]
P04063	4.1e-161	62.56	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3	[more]
P27934	2.7e-160	60.19	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]

BLAST of PI0018452 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA4	2.7e-232	88.07	Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1	[more]
A0A6J1FCS4	7.9e-224	85.68	Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1	[more]
A0A6J1IGI6	1.8e-220	86.35	Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1	[more]
A0A1S3V9G5	1.1e-193	76.32	Alpha-amylase OS=Vigna radiata var. radiata OX=3916 GN=LOC106772881 PE=3 SV=1	[more]
A0A2N9FNN7	1.4e-193	74.70	Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1	[more]

BLAST of PI0018452 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142476.1	5.6e-232	88.07	alpha-amylase [Momordica charantia]	[more]
XP_038896126.1	8.9e-230	90.09	alpha-amylase-like [Benincasa hispida]	[more]
XP_022936258.1	1.6e-223	85.68	alpha-amylase, partial [Cucurbita moschata]	[more]
XP_023536126.1	9.9e-221	86.38	alpha-amylase, partial [Cucurbita pepo subsp. pepo]	[more]
XP_022976296.1	3.7e-220	86.35	alpha-amylase [Cucurbita maxima]	[more]

BLAST of PI0018452 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25000.1	1.2e-163	66.83	alpha-amylase-like	[more]
AT1G69830.1	8.9e-103	46.15	alpha-amylase-like 3	[more]
AT1G76130.1	4.9e-101	45.27	alpha-amylase-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 253..264 score: 40.85 coord: 357..369 score: 51.43 coord: 161..172 score: 49.51
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 82..419 e-value: 4.0E-49 score: 179.2
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 114..400 e-value: 2.1E-14 score: 53.8
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 420..480 e-value: 3.2E-31 score: 119.7
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 421..479 e-value: 5.9E-17 score: 61.7
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 424..481 e-value: 1.2E-22 score: 81.7
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 82..423 e-value: 1.7E-143 score: 480.3
None	No IPR available	PIRSR	PIRSR001021-2	PIRSR001021-2	coord: 227..426 e-value: 2.5E-15 score: 54.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 207..232
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 59..481
None	No IPR available	PANTHER	PTHR43447:SF37	ALPHA-AMYLASE 1	coord: 59..481
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 84..430 e-value: 1.34773E-167 score: 472.476
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 424..480
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 83..411

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0018452.1	PI0018452.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding