PI0018449 (gene) Melon (PI 482460) v1

Overview


Name	PI0018449
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Proteinase inhibitor 1
Location	chr08: 2277399 .. 2277839 (-)
RNA-Seq Expression	PI0018449
Synteny	PI0018449

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTTTGTAAGGTCACAAATAGAAAGATAACTATTCCGAGATCATGTCGTCCCCATACGTTCGTAAGTATATCTTTTGATGTAATTTTTTTTGTTGAGTTCATCTCTTTTGTTCTAAGTTATATATATACAAACTTTTTAATCTTTGCTAATTAACAATAATATATATACTTTTTGCCAATATGTTTCAAAACAGTAAAATCATGGCCAGAACTAAACTTCATGGATGCAAACACTGTGGCAAATATCATCAAGAGAGAGAATCCTAGCTTTCAACCAGTCATATTGTTGGCAGGAAGTCCAGTAACAATGGATCTTAAACATGGTCGAGTTCGACTCTTTACTAATGTGCAGGGAACAGTGGTTGTTATTCCTCAAGAGGGCTGAAATGTAAACATACATGTTTCAAATAATAAATAAAAGTAATAAACTATTTCTAATA

mRNA sequence

AACTTTGTAAGGTCACAAATAGAAAGATAACTATTCCGAGATCATGTCGTCCCCATACGTTCTAAAATCATGGCCAGAACTAAACTTCATGGATGCAAACACTGTGGCAAATATCATCAAGAGAGAGAATCCTAGCTTTCAACCAGTCATATTGTTGGCAGGAAGTCCAGTAACAATGGATCTTAAACATGGTCGAGTTCGACTCTTTACTAATGTGCAGGGAACAGTGGTTGTTATTCCTCAAGAGGGCTGAAATGTAAACATACATGTTTCAAATAATAAATAAAAGTAATAAACTATTTCTAATA

Coding sequence (CDS)

ATGTCGTCCCCATACGTTCTAAAATCATGGCCAGAACTAAACTTCATGGATGCAAACACTGTGGCAAATATCATCAAGAGAGAGAATCCTAGCTTTCAACCAGTCATATTGTTGGCAGGAAGTCCAGTAACAATGGATCTTAAACATGGTCGAGTTCGACTCTTTACTAATGTGCAGGGAACAGTGGTTGTTATTCCTCAAGAGGGCTGA

Protein sequence

MSSPYVLKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQEG

Homology

[Close]

BLAST of PI0018449 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.5e-08
Identity = 31/59 (52.54%), Postives = 38/59 (64.41%), Query Frame = 0


Query: 10  WPELNFMDANTVANIIKRENPSFQPV-ILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQ 68
           WPEL  + A     II++ENPS   + ILL GSPVT D +  RVRLF N+ G VV IP+
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

[Close]

BLAST of PI0018449 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.9e-07
Identity = 28/58 (48.28%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 10  WPELNFMDANTVANIIKRENPSFQPV-ILLAGSPVTMDLKHGRVRLFTNVQGTVVVIP 67
           WPEL  +       II++ENPS   + ILL+GSP+T+D    RVRLF N+ G VV +P
Sbjct: 51  WPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQMP 108

[Close]

BLAST of PI0018449 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 6.3e-07
Identity = 30/59 (50.85%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 10  WPELNFMDANTVANIIKRENPSFQPV-ILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQ 68
           WPEL  +       II++EN     V ILL GSPVTMD +  RVRLF N+ G VV IP+
Sbjct: 47  WPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVVQIPR 105

[Close]

BLAST of PI0018449 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 8.3e-07
Identity = 30/61 (49.18%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 10  WPELNFMDANTVANIIKRENPSFQPV-ILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQE 69
           WPEL  +       II++EN     V ILL GSPVT+D++  RVRLF N+ G VV IP  
Sbjct: 47  WPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVDIPVV 106

[Close]

BLAST of PI0018449 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-06
Identity = 26/61 (42.62%), Postives = 38/61 (62.30%), Query Frame = 0


Query: 9  SWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQE 68
          SWP L  +  +    II+R+NP+ + VIL  G+PVT D +  RVR++ N +G VV  P+ 
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPPRI 67

Query: 69 G 70
          G
Sbjct: 68 G 68

[Close]

BLAST of PI0018449 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.4e-20
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 2  SSPYVLKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGT 61
          +S + LKSWP LNFMDA TVANIIK+ENP FQ  ILLAGSPVT DLKHGRVRLFTN++  
Sbjct: 4  ASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEI 63

Query: 62 VVVIPQEG 70
          VV++PQEG
Sbjct: 64 VVIVPQEG 71

[Close]

BLAST of PI0018449 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.6e-13
Identity = 40/69 (57.97%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 1  MSSPYVLKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQG 60
          MS P+ LKSWP L F+DA TVAN IK ENP+F+ V +  G+PVT D+  GRVRL  +  G
Sbjct: 1  MSFPFNLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANG 60

Query: 61 TVVVIPQEG 70
           VV +PQEG
Sbjct: 61 KVVNVPQEG 69

[Close]

BLAST of PI0018449 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-08
Identity = 30/61 (49.18%), Postives = 43/61 (70.49%), Query Frame = 0


Query: 9  SWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQE 68
          SWPEL F+ ++   + I+R+ P  +PV+LLAGSPVT DL+  RVR+F N+   VV +P+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 69 G 70
          G
Sbjct: 75 G 75

[Close]

BLAST of PI0018449 vs. ExPASy TrEMBL
Match: A0A6J1CY53 (inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=LOC111015335 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.7e-08
Identity = 31/62 (50.00%), Postives = 42/62 (67.74%), Query Frame = 0


Query: 8  KSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQ 67
          + WPEL  +  +  A IIKREN   +P +LLAGSPVT+D +  RVR+F N++  VV IP+
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 68 EG 70
           G
Sbjct: 78 TG 79

[Close]

BLAST of PI0018449 vs. ExPASy TrEMBL
Match: A0A397ZK38 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 8.6e-07
Identity = 30/63 (47.62%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 7  LKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIP 66
          LK+WPEL     +  A++IKRENPSF   +LL GS VT DL+  RVR++ +    VV +P
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 67 QEG 70
            G
Sbjct: 63 TTG 65

[Close]

BLAST of PI0018449 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 108.2 bits (269), Expect = 2.8e-20
Identity = 51/68 (75.00%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 2  SSPYVLKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGT 61
          +S + LKSWP LNFMDA TVANIIK+ENP FQ  ILLAGSPVT DLKHGRVRLFTN++  
Sbjct: 4  ASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEI 63

Query: 62 VVVIPQEG 70
          VV++PQEG
Sbjct: 64 VVIVPQEG 71

[Close]

BLAST of PI0018449 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 104.8 bits (260), Expect = 3.1e-19
Identity = 50/65 (76.92%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 5  YVLKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVV 64
          YVLKSWPELN M A+TV NIIKR++P FQ VILLAGSPVT DLKHGRVRLF N+ G VV 
Sbjct: 3  YVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVE 62

Query: 65 IPQEG 70
          IP+EG
Sbjct: 63 IPREG 67

[Close]

BLAST of PI0018449 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 83.6 bits (205), Expect = 7.5e-13
Identity = 40/69 (57.97%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 1  MSSPYVLKSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQG 60
          MS P+ LKSWP L F+DA TVAN IK ENP+F+ V +  G+PVT D+  GRVRL  +  G
Sbjct: 1  MSFPFNLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANG 60

Query: 61 TVVVIPQEG 70
           VV +PQEG
Sbjct: 61 KVVNVPQEG 69

[Close]

BLAST of PI0018449 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 68.6 bits (166), Expect = 2.5e-08
Identity = 30/61 (49.18%), Postives = 43/61 (70.49%), Query Frame = 0


Query: 9  SWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQE 68
          SWPEL F+ ++   + I+R+ P  +PV+LLAGSPVT DL+  RVR+F N+   VV +P+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 69 G 70
          G
Sbjct: 75 G 75

[Close]

BLAST of PI0018449 vs. NCBI nr
Match: XP_022146026.1 (inhibitor of trypsin and hageman factor-like [Momordica charantia])

HSP 1 Score: 67.4 bits (163), Expect = 5.5e-08
Identity = 31/62 (50.00%), Postives = 42/62 (67.74%), Query Frame = 0


Query: 8  KSWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQ 67
          + WPEL  +  +  A IIKREN   +P +LLAGSPVT+D +  RVR+F N++  VV IP+
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 68 EG 70
           G
Sbjct: 78 TG 79

[Close]

BLAST of PI0018449 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 55.8 bits (133), Expect = 1.5e-08
Identity = 26/61 (42.62%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 9  SWPELNFMDANTVANIIKRENPSFQPVILLAGSPVTMDLKHGRVRLFTNVQGTVVVIPQE 68
          SWPEL   + +  A +I+RENP+    ++L GSPVT D +  RVR+F +    VV  P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 69 G 70
          G
Sbjct: 70 G 70

The following BLAST results are available for this feature:

BLAST of PI0018449 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	7.5e-08	52.54	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P05118	4.9e-07	48.28	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
P08454	6.3e-07	50.85	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]
Q00783	8.3e-07	49.18	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P19873	1.1e-06	42.62	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of PI0018449 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	1.4e-20	75.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	3.6e-13	57.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	1.2e-08	49.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A6J1CY53	2.7e-08	50.00	inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A397ZK38	8.6e-07	47.62	Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1	[more]

BLAST of PI0018449 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN60477.1	2.8e-20	75.00	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KAG6571807.1	3.1e-19	76.92	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60478.1	7.5e-13	57.97	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	2.5e-08	49.18	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
XP_022146026.1	5.5e-08	50.00	inhibitor of trypsin and hageman factor-like [Momordica charantia]	[more]

BLAST of PI0018449 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.5e-08	42.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 9..22 score: 32.92 coord: 34..45 score: 48.19 coord: 46..56 score: 47.04
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..67 e-value: 2.9E-15 score: 56.7
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 7..69
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 6..67 e-value: 2.7E-16 score: 61.6
None	No IPR available	PANTHER	PTHR33091:SF69	SUBFAMILY NOT NAMED	coord: 7..69
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 7..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0018449.1	PI0018449.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity