PI0017526 (gene) Melon (PI 482460) v1

Overview
NamePI0017526
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
Locationchr11: 29016959 .. 29018700 (-)
RNA-Seq ExpressionPI0017526
SyntenyPI0017526
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGCCATTTCATGTAATTTGGAAGTGACGAGTGGTTTTTTCATTAAGCTTTCTCTTTACTCTCACTATATATAAAGCTTTCCCACAGCCTTCAATGGAGGACATGGCCAGGACTGCTTAAACAACAACCTCTCCATAGTTCCCTAAATTTCCTTTTTTTTTTTCTTTTTTTTTCTTCCATTTTTCAGAGAAGAAAATGTTGAAGAAGATGAAGTTTTTCTCTCCCTTCTTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCTGGGTCTTACTAGTAAGCTTCTCTCTCTCTATCTCTCTTTACATCTATTTACTTCCTGTCTTGTGGGTTTATTGTTCTGCCATGGCAGCTGCAGGTCAAGAATCCATTCAATTTGTGAAGCTGGATGAGATAGTTTCTTCAGGGGATTTTCCAGCAATGTCCGCTTCGGAACTCCCTGTTGCTGTTTCTGTTACTGATGTTGAACTTCCAGAGGTTTCAAGCAGTGTTTTAATGGCGGAAAGTTGGCTTAGAACCTTCGTTCTGGCTCACTACCCTTCCACTAAAATCACCACCATTGTTGTGGGAAATAGTTCTTTATGCAGCAACAAGAATCTGGATACTAACAATTTACACATTGTGTTGCTGTCTGTGAAGAATTTGTTTTACTCCCTCACTAGATGGGGTTTGGAGAATCAAATCAAAGTTTCTACTCTCTTCCCAAAAGATTGTTTCCACTCTCAAGAGGAATCCATTCAGAATATGGTTAAGCTTGTGATTGAGTTCATCCAAAGTACTAACTCTACATTGAGTCTCAAACTACCTGAAAATGTAATTTCTCTACATGAAACAGAGAGTTTCATCTCCACCCACATGAACAAATTTGGATTTCTAAAGCTAAACAAAGTGAATTTATTAACCAGCGTCTCAAAACAAAGAAACCCCATTAATAGAAAACTCTCTTCATTCATGGAACTCAAAGAATACGAACCTTTCTTTACATCAGAGCCACCATTGCCATCGGATCTCGCCACACCTCCACTTCCACCAGAGTCCCAAATCGCATCTCCGCCACATTGGAGCTTCGCCGCCGCTCCAGAATCGCCTCCGTTCGTGGTACCAGCAAGCCCGCCAATGGGATTTACCCTGCCTCCATGTAATCCAGACCAAAACGCCGGTGCCCCATTTCCACAAACAGGTGGTGTGCAAAAACTGTGGTGCGTGGCGAAACCAAGTGTTCCGGCTGAGATTCTTCAGCAGGCGATGGATTACGCATGTGGGGACGGCGGCGCCGACTGCCGGGAGATATCGGCGGAAGGTAGCTGCTTTCATCCGGATAGTTTGGTGGCACATGCATCGTACGCTTTCAATAGCTACTGGCAGAAGAACAAGAGAAGTGGAGGAACTTGTAGCTTTGGAGGAACTGCCATGATTATAAGCGCAGATCCAAGTAAGAATTTGTCTGATTTTATGAATTTTTTTTTTTGTGTATGAATCAATGATGAATTTTGATGAATTGGGTTTTGTTGTATTGATTTGTTTTTTGCAGGTTTTAATCACTGCCGGTTTGTTCTAAGCTAAATTTTTTAGGGATAATGGCGTTTAGTGTTTGTTCTTCTTCTTTGTTCTTGTTCTTGTTATGTTCATCTTGTTTCTTTGAATAATACTTTATAATATCAACTTTGAAGAGACTTTGTGACATTGGCCTTAGAGGTTTTGGTAATTTTTGTTTTTATTTTAATTAAAAAATAATAGGG

mRNA sequence

TTTTGCCATTTCATGTAATTTGGAAGTGACGAGTGGTTTTTTCATTAAGCTTTCTCTTTACTCTCACTATATATAAAGCTTTCCCACAGCCTTCAATGGAGGACATGGCCAGGACTGCTTAAACAACAACCTCTCCATAGTTCCCTAAATTTCCTTTTTTTTTTTCTTTTTTTTTCTTCCATTTTTCAGAGAAGAAAATGTTGAAGAAGATGAAGTTTTTCTCTCCCTTCTTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCTGGGTCTTACTACTGCAGGTCAAGAATCCATTCAATTTGTGAAGCTGGATGAGATAGTTTCTTCAGGGGATTTTCCAGCAATGTCCGCTTCGGAACTCCCTGTTGCTGTTTCTGTTACTGATGTTGAACTTCCAGAGGTTTCAAGCAGTGTTTTAATGGCGGAAAGTTGGCTTAGAACCTTCGTTCTGGCTCACTACCCTTCCACTAAAATCACCACCATTGTTGTGGGAAATAGTTCTTTATGCAGCAACAAGAATCTGGATACTAACAATTTACACATTGTGTTGCTGTCTGTGAAGAATTTGTTTTACTCCCTCACTAGATGGGGTTTGGAGAATCAAATCAAAGTTTCTACTCTCTTCCCAAAAGATTGTTTCCACTCTCAAGAGGAATCCATTCAGAATATGGTTAAGCTTGTGATTGAGTTCATCCAAAGTACTAACTCTACATTGAGTCTCAAACTACCTGAAAATGTAATTTCTCTACATGAAACAGAGAGTTTCATCTCCACCCACATGAACAAATTTGGATTTCTAAAGCTAAACAAAGTGAATTTATTAACCAGCGTCTCAAAACAAAGAAACCCCATTAATAGAAAACTCTCTTCATTCATGGAACTCAAAGAATACGAACCTTTCTTTACATCAGAGCCACCATTGCCATCGGATCTCGCCACACCTCCACTTCCACCAGAGTCCCAAATCGCATCTCCGCCACATTGGAGCTTCGCCGCCGCTCCAGAATCGCCTCCGTTCGTGGTACCAGCAAGCCCGCCAATGGGATTTACCCTGCCTCCATGTAATCCAGACCAAAACGCCGGTGCCCCATTTCCACAAACAGGTGGTGTGCAAAAACTGTGGTGCGTGGCGAAACCAAGTGTTCCGGCTGAGATTCTTCAGCAGGCGATGGATTACGCATGTGGGGACGGCGGCGCCGACTGCCGGGAGATATCGGCGGAAGGTAGCTGCTTTCATCCGGATAGTTTGGTGGCACATGCATCGTACGCTTTCAATAGCTACTGGCAGAAGAACAAGAGAAGTGGAGGAACTTGTAGCTTTGGAGGAACTGCCATGATTATAAGCGCAGATCCAAGTTTTAATCACTGCCGGTTTGTTCTAAGCTAAATTTTTTAGGGATAATGGCGTTTAGTGTTTGTTCTTCTTCTTTGTTCTTGTTCTTGTTATGTTCATCTTGTTTCTTTGAATAATACTTTATAATATCAACTTTGAAGAGACTTTGTGACATTGGCCTTAGAGGTTTTGGTAATTTTTGTTTTTATTTTAATTAAAAAATAATAGGG

Coding sequence (CDS)

ATGTTGAAGAAGATGAAGTTTTTCTCTCCCTTCTTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCTGGGTCTTACTACTGCAGGTCAAGAATCCATTCAATTTGTGAAGCTGGATGAGATAGTTTCTTCAGGGGATTTTCCAGCAATGTCCGCTTCGGAACTCCCTGTTGCTGTTTCTGTTACTGATGTTGAACTTCCAGAGGTTTCAAGCAGTGTTTTAATGGCGGAAAGTTGGCTTAGAACCTTCGTTCTGGCTCACTACCCTTCCACTAAAATCACCACCATTGTTGTGGGAAATAGTTCTTTATGCAGCAACAAGAATCTGGATACTAACAATTTACACATTGTGTTGCTGTCTGTGAAGAATTTGTTTTACTCCCTCACTAGATGGGGTTTGGAGAATCAAATCAAAGTTTCTACTCTCTTCCCAAAAGATTGTTTCCACTCTCAAGAGGAATCCATTCAGAATATGGTTAAGCTTGTGATTGAGTTCATCCAAAGTACTAACTCTACATTGAGTCTCAAACTACCTGAAAATGTAATTTCTCTACATGAAACAGAGAGTTTCATCTCCACCCACATGAACAAATTTGGATTTCTAAAGCTAAACAAAGTGAATTTATTAACCAGCGTCTCAAAACAAAGAAACCCCATTAATAGAAAACTCTCTTCATTCATGGAACTCAAAGAATACGAACCTTTCTTTACATCAGAGCCACCATTGCCATCGGATCTCGCCACACCTCCACTTCCACCAGAGTCCCAAATCGCATCTCCGCCACATTGGAGCTTCGCCGCCGCTCCAGAATCGCCTCCGTTCGTGGTACCAGCAAGCCCGCCAATGGGATTTACCCTGCCTCCATGTAATCCAGACCAAAACGCCGGTGCCCCATTTCCACAAACAGGTGGTGTGCAAAAACTGTGGTGCGTGGCGAAACCAAGTGTTCCGGCTGAGATTCTTCAGCAGGCGATGGATTACGCATGTGGGGACGGCGGCGCCGACTGCCGGGAGATATCGGCGGAAGGTAGCTGCTTTCATCCGGATAGTTTGGTGGCACATGCATCGTACGCTTTCAATAGCTACTGGCAGAAGAACAAGAGAAGTGGAGGAACTTGTAGCTTTGGAGGAACTGCCATGATTATAAGCGCAGATCCAAGTTTTAATCACTGCCGGTTTGTTCTAAGCTAA

Protein sequence

MLKKMKFFSPFFLLFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVLLSVKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS
Homology
BLAST of PI0017526 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 98.6 bits (244), Expect = 1.7e-19
Identity = 104/388 (26.80%), Postives = 173/388 (44.59%), Query Frame = 0

Query: 50  AMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKN 109
           A++ +++ V +SV + +L  + SS   A SW+   V+A+YP T IT I VG+  L +   
Sbjct: 85  ALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLITAISVGDEVLTT--- 144

Query: 110 LDTNNLHIVLLSVKNLFYSLTRWGLENQIKVST---------LFPKDCFHSQEESIQNMV 169
              ++  ++L ++++L+ +L    L  QIKVST          FP    +  +     MV
Sbjct: 145 -VPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQAYFNQTWHSIMV 204

Query: 170 KLVIEFIQSTNSTLSLKLPENVI-----------------------------SLHET--- 229
            L ++F+  T S L + L    +                              LH T   
Sbjct: 205 PL-LQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDPNTLLHYTNVL 264

Query: 230 ESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPLPSDLA 289
           ++ +         L ++ V +L + S   +  + K   +  +   + + ++      D  
Sbjct: 265 DAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSK-EPYATIDNADTYNSNLIKHVFDRT 324

Query: 290 TPPLPPESQIASPPHWSFAAAPESPPFVVPASPPM--GFTLPPCNPDQNAGAPFPQTGGV 349
             PL PE   +   +  F     +PP V  AS  +  G + P      +    F      
Sbjct: 325 GTPLHPEMTSSVYIYELFNEDLRAPP-VSEASWGLFYGNSTPVYLLHVSGSGTFLANDTT 384

Query: 350 QKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQK 395
            + +C+A   V A+ LQ A+D+ACG G ++C EI    SC+ P+++  HAS+AFNSY+QK
Sbjct: 385 NQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQK 444

BLAST of PI0017526 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-19
Identity = 105/421 (24.94%), Postives = 177/421 (42.04%), Query Frame = 0

Query: 31  QESIQFVKLDEIVSSGDFPAMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYP 90
           Q +I++V++ +  +S    A   + + + + V + +L   S S    ++WL+  VL +YP
Sbjct: 49  QHNIKYVRIYD-YNSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYP 108

Query: 91  STKITTIVVGNSSLCSNKNLDTNNLHIVLLSVKNLFYSLTRWGLENQIKVSTL------- 150
           +TKIT I VG     S  +   N    V+ +++N+  +L + GL  +IKVST        
Sbjct: 109 TTKITYITVGAE---STDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILS 168

Query: 151 --FPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL---------PENV---ISLHETE 210
             FP     +   S    ++ ++EF+    S   + L         P NV     L E+ 
Sbjct: 169 RSFPPSA-GAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESS 228

Query: 211 SFI------STHMNKFG------FLKLNKVNLL--------TSVSKQRNPINRKLSSFME 270
           S +        + N F       +  L  +N          T    + +P  +  +S   
Sbjct: 229 SEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDN 288

Query: 271 LKEYEPFFTSEPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPP 330
            + Y         + ++  TP  P E+       + F+   E+    + +    G     
Sbjct: 289 AETYNSNIIRH--VVTNQGTPAKPGEAMNV----YIFSLFNENRKAGLDSERNWGLFY-- 348

Query: 331 CNPDQNAGAPFPQTG---------------GVQKLWCVAKPSVPAEILQQAMDYACGDGG 390
             PDQ +      TG               G    WC+A        L+ A+D+ACG G 
Sbjct: 349 --PDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGN 408

Query: 391 ADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRF 396
            DC  I     CF PD+LV+HAS+ FNSY+Q+N+ +   CSFGG  + ++ DPS++ C +
Sbjct: 409 VDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIY 454

BLAST of PI0017526 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.2e-18
Identity = 47/110 (42.73%), Postives = 60/110 (54.55%), Query Frame = 0

Query: 286 TLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGS 345
           T  P  P  ++  P P T G  K WCV K       LQ  +DY C   G DC  I A G+
Sbjct: 14  TPTPSVPTSSSPVPKPPTQG-NKKWCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGA 73

Query: 346 CFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFV 396
           CF+P+++ AHASYA NS++Q   R+   C F GT  I S+DPS   C F+
Sbjct: 74  CFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPSNGSCSFL 122

BLAST of PI0017526 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.2e-18
Identity = 40/91 (43.96%), Postives = 59/91 (64.84%), Query Frame = 0

Query: 304 GGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSY 363
           G     WCV K  +   +LQ+ +DYACG+ GADC     +GSCF+PD++ AH +YA NS+
Sbjct: 15  GHTSASWCVCKTGLSDSVLQKTLDYACGN-GADCNPTHPKGSCFNPDNVRAHCNYAVNSF 74

Query: 364 WQKNKRSGGTCSFGGTAMIISADPSFNHCRF 395
           +QK  ++  +C+F GTA + + DPS+  C F
Sbjct: 75  FQKKGQASESCNFTGTATLTTTDPSYTGCAF 104

BLAST of PI0017526 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 2.7e-17
Identity = 97/398 (24.37%), Postives = 172/398 (43.22%), Query Frame = 0

Query: 50  AMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKN 109
           A++ + + V +S+ + +L  +  S   A +W++  V+AHYP+T IT + VG+  L S   
Sbjct: 73  ALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSL-- 132

Query: 110 LDTNNLHIVLLSVKNLFYSLTRWGLENQIKVSTLF----------PKDCFHSQEESIQNM 169
             +N   +++ ++KN+  +L    L+  IKVST            P   F ++  S+  +
Sbjct: 133 --SNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNR--SLNAV 192

Query: 170 VKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNP 229
           +  ++ F+QSTNS L + +    I   ++   I      F  +  NK       +   N 
Sbjct: 193 IVPLLSFLQSTNSYLMVNV-YPYIDYMQSNGVIPLDYALFKPIPPNK------EAVDANT 252

Query: 230 INRKLSSFMELKEYEPF------FTSEPPLPSDLATPPL----PPESQIASPPHWSF--- 289
           + R  ++F  + +   F      FT+ P L ++   P       P++ + +   ++    
Sbjct: 253 LVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLI 312

Query: 290 ------AAAPESPPFVVPASPPMGFTLPPCNPDQNAGAP------------------FPQ 349
                    P+ P   V       +     N D  AG                       
Sbjct: 313 RHVLNKTGTPKRPGIAVST-----YIYELYNEDTKAGLSEKNWGLFNANGEPVYVLRLTN 372

Query: 350 TGGV------QKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHA 395
           +G V       + +C A+     ++LQ A+D+ACG G  DC  I    +C+ PD++VAHA
Sbjct: 373 SGSVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHA 432

BLAST of PI0017526 vs. ExPASy TrEMBL
Match: A0A1S3C6A1 (Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo OX=3656 GN=LOC103497386 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 6.7e-213
Identity = 386/399 (96.74%), Postives = 393/399 (98.50%), Query Frame = 0

Query: 1   MLKKMKFFSPFF-LLFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVA 60
           M+KKMKFFS FF LLFFFFFFFFLLGLTTAGQESIQFVKL+EIVSSG FPAMSASELPVA
Sbjct: 1   MVKKMKFFSTFFLLLFFFFFFFFLLGLTTAGQESIQFVKLEEIVSSGVFPAMSASELPVA 60

Query: 61  VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNK-NLDTNNLHIV 120
           VS+TDVELPEVSSSVLMAESWLRTFVLAHYPSTKI+TIVVGNSSLCSNK NLDTNNLHIV
Sbjct: 61  VSLTDVELPEVSSSVLMAESWLRTFVLAHYPSTKISTIVVGNSSLCSNKNNLDTNNLHIV 120

Query: 121 LLSVKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL 180
           LLS+KNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL
Sbjct: 121 LLSMKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL 180

Query: 181 PENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFT 240
           PENVISLHETESFISTH NKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEY+PFFT
Sbjct: 181 PENVISLHETESFISTHTNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFT 240

Query: 241 SEPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGA 300
           SEPPLPSDLATPPLPPESQIASPPHW FAAAPESPPFVVPASPPMGFTLPPCNPDQNAGA
Sbjct: 241 SEPPLPSDLATPPLPPESQIASPPHWGFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGA 300

Query: 301 PFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASY 360
           PFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASY
Sbjct: 301 PFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASY 360

Query: 361 AFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           AFNSYWQKNKRSGGTCSFGGTAMIIS+DPSFNHCRFVLS
Sbjct: 361 AFNSYWQKNKRSGGTCSFGGTAMIISSDPSFNHCRFVLS 399

BLAST of PI0017526 vs. ExPASy TrEMBL
Match: A0A5A7TQX1 (Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00660 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.2e-196
Identity = 353/371 (95.15%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 26  LTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFV 85
           +  AGQESIQFVKL+EIVSSG FPAMSASELPVAVS+TDVELPEVSSSVLMAESWLRTFV
Sbjct: 1   MAAAGQESIQFVKLEEIVSSGVFPAMSASELPVAVSLTDVELPEVSSSVLMAESWLRTFV 60

Query: 86  LAHYPSTKITTIVVGNSSLCSNK-NLDTNNLHIVLLSVKNLFYSLTRWGLENQIKVSTLF 145
           LAHYPSTKI+TIVVGNSSLCSNK NLDTNNLHIVLLS+KNLFYSLTRWGLENQIKVSTLF
Sbjct: 61  LAHYPSTKISTIVVGNSSLCSNKNNLDTNNLHIVLLSMKNLFYSLTRWGLENQIKVSTLF 120

Query: 146 PKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHMNKFGFLKL 205
           PKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTH NKFGFLKL
Sbjct: 121 PKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHTNKFGFLKL 180

Query: 206 NKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPLPSDLATPPLPPESQIASPPHW 265
           NKVNLLTSVSKQRNPINRKLSSFMELKEY+PFFTSEPPLPSDLATPPLPPESQIASPPHW
Sbjct: 181 NKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFTSEPPLPSDLATPPLPPESQIASPPHW 240

Query: 266 SFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ 325
            FAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ
Sbjct: 241 GFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ 300

Query: 326 AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS 385
           AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS
Sbjct: 301 AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS 360

Query: 386 ADPSFNHCRFV 396
           +DPS N   F+
Sbjct: 361 SDPSKNLSDFL 371

BLAST of PI0017526 vs. ExPASy TrEMBL
Match: A0A0A0LLS7 (Glucan endo-1,3-beta-D-glucosidase OS=Cucumis sativus OX=3659 GN=Csa_2G277600 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.3e-191
Identity = 339/347 (97.69%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 51  MSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNL 110
           MSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNL
Sbjct: 1   MSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNL 60

Query: 111 DTNNLHIVLLSVKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQST 170
           DTNNLHIVLLS+KNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQST
Sbjct: 61  DTNNLHIVLLSMKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQST 120

Query: 171 NSTLSLKLPENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMEL 230
           NSTLSLKLPENVISLHETESFISTH NKFGFLKL KVNLLTSVSKQRNP NRKLSSFMEL
Sbjct: 121 NSTLSLKLPENVISLHETESFISTHTNKFGFLKLKKVNLLTSVSKQRNPTNRKLSSFMEL 180

Query: 231 KEYEPFFTSEPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPC 290
           KEYEPFFTSEPPLPSDLATPPLPPESQIASPPHWSFA+APESPPFVVPASPPMGFTLPPC
Sbjct: 181 KEYEPFFTSEPPLPSDLATPPLPPESQIASPPHWSFASAPESPPFVVPASPPMGFTLPPC 240

Query: 291 NPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPD 350
           NP+QNAGAPFPQTGGVQKLWCVAKP+VPAEILQQAMDYACGDGGADCREISAEGSCFHPD
Sbjct: 241 NPNQNAGAPFPQTGGVQKLWCVAKPNVPAEILQQAMDYACGDGGADCREISAEGSCFHPD 300

Query: 351 SLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           SLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS+DPSFNHCRFVLS
Sbjct: 301 SLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISSDPSFNHCRFVLS 347

BLAST of PI0017526 vs. ExPASy TrEMBL
Match: A0A6J1EY45 (Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111439476 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 8.9e-181
Identity = 326/387 (84.24%), Postives = 351/387 (90.70%), Query Frame = 0

Query: 11  FFLLFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVTDVELPEV 70
           FF  FFFFFFFFLLGLTTAGQESIQF+KLDE +SSG  P +SAS+LPVAVSVTD ELPEV
Sbjct: 12  FFFFFFFFFFFFLLGLTTAGQESIQFLKLDEKISSGVSPPISASQLPVAVSVTDAELPEV 71

Query: 71  SSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVLLSVKNLFYSLT 130
           SSSVLMAE+WLR FVLAHYP+T ITTIVVGNSSLC   N DTN+LH VLLS+KNLFYSLT
Sbjct: 72  SSSVLMAETWLRAFVLAHYPATNITTIVVGNSSLC---NQDTNHLHSVLLSLKNLFYSLT 131

Query: 131 RWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETES 190
           RWGLENQIKVSTLFP DCFHS+     NM+ LVIEFI++TNST +LKLP+NV+SLHET+S
Sbjct: 132 RWGLENQIKVSTLFPDDCFHSKP---HNMLNLVIEFIKTTNSTFTLKLPQNVLSLHETQS 191

Query: 191 FISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPLPSDLATP 250
           F+ST M KFGFLKLNKVNL+T+VSKQRNP +RKL SFMEL EYE FFTSEPPLPSDLA P
Sbjct: 192 FVSTRMAKFGFLKLNKVNLITTVSKQRNPFSRKLFSFMELNEYESFFTSEPPLPSDLAIP 251

Query: 251 PLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLW 310
           P PPESQIASPPHW FAAAPESPPFVVPASPP+GF LPPCNPDQN GAPFPQTGGVQK W
Sbjct: 252 PNPPESQIASPPHWGFAAAPESPPFVVPASPPLGFVLPPCNPDQNVGAPFPQTGGVQKQW 311

Query: 311 CVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRS 370
           CVAKPSVPAEILQQAMDYACG GGADCREISA+G CFHPD+LVAHASYAFNSYWQKNKRS
Sbjct: 312 CVAKPSVPAEILQQAMDYACGQGGADCREISADGDCFHPDTLVAHASYAFNSYWQKNKRS 371

Query: 371 GGTCSFGGTAMIISADPSFNHCRFVLS 398
           GGTCSFGGTAMIIS+DPSF+HCRFVLS
Sbjct: 372 GGTCSFGGTAMIISSDPSFHHCRFVLS 392

BLAST of PI0017526 vs. ExPASy TrEMBL
Match: A0A6J1JGG8 (Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111484177 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.3e-175
Identity = 320/390 (82.05%), Postives = 346/390 (88.72%), Query Frame = 0

Query: 8   FSPFFLLFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVTDVEL 67
           F+   L  FFF FFFLLGLTTAGQESIQF+KL + +SSG  P +SAS+LP+AVSVTD EL
Sbjct: 4   FNNNLLCSFFFSFFFLLGLTTAGQESIQFLKLYDKISSGVSPPISASQLPIAVSVTDAEL 63

Query: 68  PEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVLLSVKNLFY 127
           PEVSSSVLMAE+WLR FVLAHYP+T ITTIVVGNSSLC   N DTN+LH VLLS+KNLFY
Sbjct: 64  PEVSSSVLMAETWLRAFVLAHYPATSITTIVVGNSSLC---NQDTNHLHSVLLSLKNLFY 123

Query: 128 SLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHE 187
           SLTRWGLENQIKVSTLFP+DCFHS      NM+ LV EFI++TNST +LKLP+NVISLHE
Sbjct: 124 SLTRWGLENQIKVSTLFPEDCFHSNP---HNMLNLVTEFIKTTNSTFTLKLPQNVISLHE 183

Query: 188 TESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPLPSDL 247
           T+SF+ST M KFG LKLNKVNL+T VSKQRNP +RKL SFMEL EYE FFTSEPPLPSDL
Sbjct: 184 TQSFVSTRMAKFGILKLNKVNLITRVSKQRNPFSRKLFSFMELNEYEYFFTSEPPLPSDL 243

Query: 248 ATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQ 307
           ATPP PPESQIASPPHW FAAAPESPPFVVPASPP+ F LPPCNPDQN GAPFPQTGGVQ
Sbjct: 244 ATPPNPPESQIASPPHWGFAAAPESPPFVVPASPPLSFVLPPCNPDQNVGAPFPQTGGVQ 303

Query: 308 KLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKN 367
           K WCVAKPSVPAEILQQAMDYACG GGADCREISA+G CFHPD+LVAHASYAFNSYWQKN
Sbjct: 304 KQWCVAKPSVPAEILQQAMDYACGQGGADCREISADGDCFHPDTLVAHASYAFNSYWQKN 363

Query: 368 KRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           KRSGGTCSFGGTAMIIS+DPSF+HCRFVLS
Sbjct: 364 KRSGGTCSFGGTAMIISSDPSFHHCRFVLS 387

BLAST of PI0017526 vs. NCBI nr
Match: XP_004149701.2 (glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus] >KAE8651912.1 hypothetical protein Csa_006335 [Cucumis sativus])

HSP 1 Score: 754.2 bits (1946), Expect = 5.6e-214
Identity = 386/398 (96.98%), Postives = 392/398 (98.49%), Query Frame = 0

Query: 1   MLKKMKFFSPFFLLFFFFFFFF-LLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVA 60
           M+KKMKFFSPFFLLFFFFFFFF LLGLTTAGQESIQFVKLDEI SSG FPAMSASELPVA
Sbjct: 1   MVKKMKFFSPFFLLFFFFFFFFLLLGLTTAGQESIQFVKLDEIASSGVFPAMSASELPVA 60

Query: 61  VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVL 120
           VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVL
Sbjct: 61  VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVL 120

Query: 121 LSVKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLP 180
           LS+KNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLP
Sbjct: 121 LSMKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLP 180

Query: 181 ENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTS 240
           ENVISLHETESFISTH NKFGFLKL KVNLLTSVSKQRNP NRKLSSFMELKEYEPFFTS
Sbjct: 181 ENVISLHETESFISTHTNKFGFLKLKKVNLLTSVSKQRNPTNRKLSSFMELKEYEPFFTS 240

Query: 241 EPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAP 300
           EPPLPSDLATPPLPPESQIASPPHWSFA+APESPPFVVPASPPMGFTLPPCNP+QNAGAP
Sbjct: 241 EPPLPSDLATPPLPPESQIASPPHWSFASAPESPPFVVPASPPMGFTLPPCNPNQNAGAP 300

Query: 301 FPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYA 360
           FPQTGGVQKLWCVAKP+VPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYA
Sbjct: 301 FPQTGGVQKLWCVAKPNVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYA 360

Query: 361 FNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           FNSYWQKNKRSGGTCSFGGTAMIIS+DPSFNHCRFVLS
Sbjct: 361 FNSYWQKNKRSGGTCSFGGTAMIISSDPSFNHCRFVLS 398

BLAST of PI0017526 vs. NCBI nr
Match: XP_008457775.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo])

HSP 1 Score: 749.6 bits (1934), Expect = 1.4e-212
Identity = 386/399 (96.74%), Postives = 393/399 (98.50%), Query Frame = 0

Query: 1   MLKKMKFFSPFF-LLFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVA 60
           M+KKMKFFS FF LLFFFFFFFFLLGLTTAGQESIQFVKL+EIVSSG FPAMSASELPVA
Sbjct: 1   MVKKMKFFSTFFLLLFFFFFFFFLLGLTTAGQESIQFVKLEEIVSSGVFPAMSASELPVA 60

Query: 61  VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNK-NLDTNNLHIV 120
           VS+TDVELPEVSSSVLMAESWLRTFVLAHYPSTKI+TIVVGNSSLCSNK NLDTNNLHIV
Sbjct: 61  VSLTDVELPEVSSSVLMAESWLRTFVLAHYPSTKISTIVVGNSSLCSNKNNLDTNNLHIV 120

Query: 121 LLSVKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL 180
           LLS+KNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL
Sbjct: 121 LLSMKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKL 180

Query: 181 PENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFT 240
           PENVISLHETESFISTH NKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEY+PFFT
Sbjct: 181 PENVISLHETESFISTHTNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFT 240

Query: 241 SEPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGA 300
           SEPPLPSDLATPPLPPESQIASPPHW FAAAPESPPFVVPASPPMGFTLPPCNPDQNAGA
Sbjct: 241 SEPPLPSDLATPPLPPESQIASPPHWGFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGA 300

Query: 301 PFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASY 360
           PFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASY
Sbjct: 301 PFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASY 360

Query: 361 AFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           AFNSYWQKNKRSGGTCSFGGTAMIIS+DPSFNHCRFVLS
Sbjct: 361 AFNSYWQKNKRSGGTCSFGGTAMIISSDPSFNHCRFVLS 399

BLAST of PI0017526 vs. NCBI nr
Match: XP_031737286.1 (glucan endo-1,3-beta-glucosidase 12 isoform X2 [Cucumis sativus])

HSP 1 Score: 743.4 bits (1918), Expect = 1.0e-210
Identity = 383/398 (96.23%), Postives = 390/398 (97.99%), Query Frame = 0

Query: 1   MLKKMKFFSPFFLLFFFFFFFF-LLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVA 60
           M+KKMKFFSPFFLLFFFFFFFF LLGLT+  QESIQFVKLDEI SSG FPAMSASELPVA
Sbjct: 1   MVKKMKFFSPFFLLFFFFFFFFLLLGLTS--QESIQFVKLDEIASSGVFPAMSASELPVA 60

Query: 61  VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVL 120
           VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVL
Sbjct: 61  VSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVL 120

Query: 121 LSVKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLP 180
           LS+KNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLP
Sbjct: 121 LSMKNLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLP 180

Query: 181 ENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTS 240
           ENVISLHETESFISTH NKFGFLKL KVNLLTSVSKQRNP NRKLSSFMELKEYEPFFTS
Sbjct: 181 ENVISLHETESFISTHTNKFGFLKLKKVNLLTSVSKQRNPTNRKLSSFMELKEYEPFFTS 240

Query: 241 EPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAP 300
           EPPLPSDLATPPLPPESQIASPPHWSFA+APESPPFVVPASPPMGFTLPPCNP+QNAGAP
Sbjct: 241 EPPLPSDLATPPLPPESQIASPPHWSFASAPESPPFVVPASPPMGFTLPPCNPNQNAGAP 300

Query: 301 FPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYA 360
           FPQTGGVQKLWCVAKP+VPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYA
Sbjct: 301 FPQTGGVQKLWCVAKPNVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYA 360

Query: 361 FNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           FNSYWQKNKRSGGTCSFGGTAMIIS+DPSFNHCRFVLS
Sbjct: 361 FNSYWQKNKRSGGTCSFGGTAMIISSDPSFNHCRFVLS 396

BLAST of PI0017526 vs. NCBI nr
Match: XP_038900662.1 (glucan endo-1,3-beta-glucosidase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 702.6 bits (1812), Expect = 1.9e-198
Identity = 357/394 (90.61%), Postives = 372/394 (94.42%), Query Frame = 0

Query: 4   KMKFFSPFFLLFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVT 63
           KMKF  P     FFFFF FLLGLTTAGQESIQFVKLDEI+SSG FPA+S S+LPVAVSVT
Sbjct: 3   KMKFLFP-----FFFFFTFLLGLTTAGQESIQFVKLDEIISSGVFPAISGSDLPVAVSVT 62

Query: 64  DVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVLLSVK 123
           D ELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCS KN DTN L  VLLS+K
Sbjct: 63  DAELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSTKNQDTNTLQFVLLSLK 122

Query: 124 NLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVI 183
           NLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTL+LKL ENVI
Sbjct: 123 NLFYSLTRWGLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLTLKLTENVI 182

Query: 184 SLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPL 243
           SLHETE+FISTHMNKFGFLKL+KVNL+TSV+ QRNPI+RKLSSFMEL EYEPFFTSEPPL
Sbjct: 183 SLHETENFISTHMNKFGFLKLHKVNLITSVTNQRNPISRKLSSFMELNEYEPFFTSEPPL 242

Query: 244 PSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQT 303
           PSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGF+LPPCNPDQNAGAPFPQT
Sbjct: 243 PSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFSLPPCNPDQNAGAPFPQT 302

Query: 304 GGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSY 363
           G VQK WCVAKPSVP EILQQAMDYACGDGGADCREISAEGSCFHPD+LVAHASYAFNSY
Sbjct: 303 GSVQKQWCVAKPSVPTEILQQAMDYACGDGGADCREISAEGSCFHPDTLVAHASYAFNSY 362

Query: 364 WQKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           WQKNKR+GGTCSFGGTAMIIS+DPSF+HCRFVLS
Sbjct: 363 WQKNKRNGGTCSFGGTAMIISSDPSFHHCRFVLS 391

BLAST of PI0017526 vs. NCBI nr
Match: KAA0045782.1 (glucan endo-1,3-beta-glucosidase 13 [Cucumis melo var. makuwa] >TYJ99499.1 glucan endo-1,3-beta-glucosidase 13 [Cucumis melo var. makuwa])

HSP 1 Score: 695.7 bits (1794), Expect = 2.4e-196
Identity = 353/371 (95.15%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 26  LTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFV 85
           +  AGQESIQFVKL+EIVSSG FPAMSASELPVAVS+TDVELPEVSSSVLMAESWLRTFV
Sbjct: 1   MAAAGQESIQFVKLEEIVSSGVFPAMSASELPVAVSLTDVELPEVSSSVLMAESWLRTFV 60

Query: 86  LAHYPSTKITTIVVGNSSLCSNK-NLDTNNLHIVLLSVKNLFYSLTRWGLENQIKVSTLF 145
           LAHYPSTKI+TIVVGNSSLCSNK NLDTNNLHIVLLS+KNLFYSLTRWGLENQIKVSTLF
Sbjct: 61  LAHYPSTKISTIVVGNSSLCSNKNNLDTNNLHIVLLSMKNLFYSLTRWGLENQIKVSTLF 120

Query: 146 PKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHMNKFGFLKL 205
           PKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTH NKFGFLKL
Sbjct: 121 PKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFISTHTNKFGFLKL 180

Query: 206 NKVNLLTSVSKQRNPINRKLSSFMELKEYEPFFTSEPPLPSDLATPPLPPESQIASPPHW 265
           NKVNLLTSVSKQRNPINRKLSSFMELKEY+PFFTSEPPLPSDLATPPLPPESQIASPPHW
Sbjct: 181 NKVNLLTSVSKQRNPINRKLSSFMELKEYDPFFTSEPPLPSDLATPPLPPESQIASPPHW 240

Query: 266 SFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ 325
            FAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ
Sbjct: 241 GFAAAPESPPFVVPASPPMGFTLPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQ 300

Query: 326 AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS 385
           AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS
Sbjct: 301 AMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIIS 360

Query: 386 ADPSFNHCRFV 396
           +DPS N   F+
Sbjct: 361 SDPSKNLSDFL 371

BLAST of PI0017526 vs. TAIR 10
Match: AT5G67460.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 272.7 bits (696), Expect = 4.6e-73
Identity = 172/393 (43.77%), Postives = 236/393 (60.05%), Query Frame = 0

Query: 14  LFFFFFFFFLLGLTTAGQESIQFVKLDEIVSSGDFPAMSASELPVAVSVT-DVELPEVSS 73
           L + F F   L    AGQES     L+  +++        ++L +AVSV+ +  + E+SS
Sbjct: 4   LLYIFLFIAFLSFAGAGQESTPIEALN--LNNNLLQLPQTTDLDLAVSVSGNKTITEISS 63

Query: 74  SVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKNLDTNNLHIVLLSVKNLFYSLTRW 133
           S++ AE+WL+T +L+ YPSTKITTIV+     C       ++  +VL S+KN+++SLTRW
Sbjct: 64  SIIKAETWLKTHILSRYPSTKITTIVIFFPDSCQTTQ---HSSDLVLSSLKNIYHSLTRW 123

Query: 134 GLENQIKVSTLFPKDCFHSQEESIQNMVKLVIEFIQSTNSTLSLKLPENVISLHETESFI 193
           GLEN IKVS+ F   C ++ + S   M K V+ F+++ NST ++  P+N ++       +
Sbjct: 124 GLENNIKVSSGFSYQCLNNPKSS--EMFKPVLIFLKTINSTFTINPPQNFLTSPHNHRDL 183

Query: 194 STHMNKFGFLKLNKVNLLTSVSKQRNP---INRKLSSFMELKEYEPFFTSEPPLPSDLAT 253
              + K G L  NKVN L    +Q +      R L S + L     F TS P LPS  + 
Sbjct: 184 LHSVEKLGSLSFNKVNFLNPEPEQESTTTMTRRNLRSLVNLS--SKFTTSFPTLPSP-SP 243

Query: 254 PPLPPESQIASPPHWSFAAAPESPPFVVPASPPM----GFTLPPCNPDQNAGAPFP-QTG 313
              P  S I SP      + P  P F  P+  PM    G +LPPC P   A +P P +  
Sbjct: 244 ETSPIHSSIGSP------SPPTIPYFPEPSQSPMESNQGISLPPCLPYHPAPSPQPVKKK 303

Query: 314 GVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHASYAFNSYW 373
            V+ LWCVAKPSV AE LQQ++D+ACG GGA+C EI   G C++PD+++AHASYAFNSYW
Sbjct: 304 NVEGLWCVAKPSVAAETLQQSLDFACGQGGANCDEIKPHGICYYPDTVMAHASYAFNSYW 363

Query: 374 QKNKRSGGTCSFGGTAMIISADPSFNHCRFVLS 398
           QK KR+GGTCSFGGTAM+I+ DPS+ HCRFVLS
Sbjct: 364 QKTKRNGGTCSFGGTAMLITTDPSYQHCRFVLS 380

BLAST of PI0017526 vs. TAIR 10
Match: AT1G79480.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 3.4e-31
Identity = 75/168 (44.64%), Postives = 94/168 (55.95%), Query Frame = 0

Query: 244 PSDLATPPLPPESQ-------IASPPHWSFAAAPE-SPPFVVPA---------SPPMGFT 303
           P+   T P PPES        + SPP   +   P   PP+  P          SP  GF 
Sbjct: 229 PNPPITIPYPPESSSPNPPEIVPSPPESGYTPGPVLGPPYSEPGPSTPTGSIPSPSSGF- 288

Query: 304 LPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSC 363
           LPP         P P        WCVAKPSVP  I+Q+AM++ACG  GADC  I   G C
Sbjct: 289 LPPIVYPPPMAPPSPSVTPTSAYWCVAKPSVPDPIIQEAMNFACG-SGADCHSIQPNGPC 348

Query: 364 FHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRF 395
           F P++L AHAS+A+NSYWQ+ K +GG+C+FGGT M+++ DPSFN C F
Sbjct: 349 FKPNTLWAHASFAYNSYWQRTKSTGGSCTFGGTGMLVTVDPSFNGCHF 394

BLAST of PI0017526 vs. TAIR 10
Match: AT1G79480.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 3.4e-31
Identity = 75/168 (44.64%), Postives = 94/168 (55.95%), Query Frame = 0

Query: 244 PSDLATPPLPPESQ-------IASPPHWSFAAAPE-SPPFVVPA---------SPPMGFT 303
           P+   T P PPES        + SPP   +   P   PP+  P          SP  GF 
Sbjct: 228 PNPPITIPYPPESSSPNPPEIVPSPPESGYTPGPVLGPPYSEPGPSTPTGSIPSPSSGF- 287

Query: 304 LPPCNPDQNAGAPFPQTGGVQKLWCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSC 363
           LPP         P P        WCVAKPSVP  I+Q+AM++ACG  GADC  I   G C
Sbjct: 288 LPPIVYPPPMAPPSPSVTPTSAYWCVAKPSVPDPIIQEAMNFACG-SGADCHSIQPNGPC 347

Query: 364 FHPDSLVAHASYAFNSYWQKNKRSGGTCSFGGTAMIISADPSFNHCRF 395
           F P++L AHAS+A+NSYWQ+ K +GG+C+FGGT M+++ DPSFN C F
Sbjct: 348 FKPNTLWAHASFAYNSYWQRTKSTGGSCTFGGTGMLVTVDPSFNGCHF 393

BLAST of PI0017526 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.8e-28
Identity = 104/398 (26.13%), Postives = 173/398 (43.47%), Query Frame = 0

Query: 50  AMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKN 109
           A++ S++ V V++ +  L   +S    A++W++T ++ ++P+T+I  I VGN        
Sbjct: 66  ALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVF----- 125

Query: 110 LDTNNLHIVLLSVKNLFYSLTRWGLENQIKVST---------LFPKDCFHSQEESIQNMV 169
           +D      ++ ++KN+  SL ++ L+  IK+S+          +P      + E I+ +V
Sbjct: 126 VDPTITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVV 185

Query: 170 KLVIEFIQSTNSTLSLKLPENVISLHETESFISTHMNKFGFLKLNKVNLLTSVSKQRNPI 229
           K ++  +Q T+S L +    N        +        +   K N  N+ +    + N +
Sbjct: 186 KPMLALLQQTSSYLMV----NAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSL 245

Query: 230 NRKLSSFMELKEYEPFFTSEPPLPSDLATPPLPPESQIASPPHWSFAAAPESPPFVVPAS 289
                  +        F     + ++   P +  E++I +       AA  +   V    
Sbjct: 246 FDAQIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGAS---ESNAAAYNAGLVKRVL 305

Query: 290 PPMGFTLPPCNP-------------------DQNAGAPFPQTGGVQKL------------ 349
              G  L P  P                   ++N G  +P  G V  +            
Sbjct: 306 TGKGTPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKKSTTPVNG 365

Query: 350 -------------WCVAKPSVPAEILQQAMDYACGDGGADCREISAEGSCFHPDSLVAHA 395
                        WCV+   V  E LQ+A+DYACG+GGADCR I    +C+HP+SL AHA
Sbjct: 366 NRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLEAHA 425

BLAST of PI0017526 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.7e-25
Identity = 107/415 (25.78%), Postives = 174/415 (41.93%), Query Frame = 0

Query: 50  AMSASELPVAVSVTDVELPEVSSSVLMAESWLRTFVLAHYPSTKITTIVVGNSSLCSNKN 109
           A+S S + V V + +  L   +     A SW++  V A++PST+I +I VGN        
Sbjct: 66  ALSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVF----- 125

Query: 110 LDTNNLHIVLL-SVKNLFYSLTRWGLENQIKVST---------LFPKDCFHSQEESIQNM 169
           +DT+N    L+ +++N+  +L  + L + IK+S+          +P      + E I ++
Sbjct: 126 VDTHNTTSFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSV 185

Query: 170 VKLVIEFIQSTNSTLSLK-------------LPENVISLHETESFIST-----HMNKF-- 229
           +K +++F++ T S L +              +P +   L E    + +     + N F  
Sbjct: 186 IKPMLDFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDA 245

Query: 230 ---------GFLKLNKVNLLTSVS--KQRNPINRKLSSFMELKEYEPFFTSEPPLPSDLA 289
                      LK + + ++ + +    +   N   ++      Y               
Sbjct: 246 QIDAVFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRR---ILTRG 305

Query: 290 TPPLPPESQIASPPHWSFAAAPESPPFVVPASPPMGFTLP-------------------- 349
             PL P++ +     + FA   E+      +    G   P                    
Sbjct: 306 GTPLRPKADLTV---YLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRD 365

Query: 350 ----PCNPDQNAGAPFPQTGGVQK-----LWCVAKPSVPAEILQQAMDYACGDGGADCRE 395
               P         P P +GGV K      WCVA      E LQ  +DYACG+GGADCR 
Sbjct: 366 GGHTPVTGGDQVTKP-PMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRP 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O653991.7e-1926.80Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q9FJU92.2e-1924.94Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q84V393.2e-1842.73Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Q9SD844.2e-1843.96PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2... [more]
Q9C7U52.7e-1724.37Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Match NameE-valueIdentityDescription
A0A1S3C6A16.7e-21396.74Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo OX=3656 GN=LOC103497386 PE=3 ... [more]
A0A5A7TQX11.2e-19695.15Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0LLS73.3e-19197.69Glucan endo-1,3-beta-D-glucosidase OS=Cucumis sativus OX=3659 GN=Csa_2G277600 PE... [more]
A0A6J1EY458.9e-18184.24Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111439476... [more]
A0A6J1JGG84.3e-17582.05Glucan endo-1,3-beta-D-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111484177 P... [more]
Match NameE-valueIdentityDescription
XP_004149701.25.6e-21496.98glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus] >KAE8651912.1 h... [more]
XP_008457775.11.4e-21296.74PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo][more]
XP_031737286.11.0e-21096.23glucan endo-1,3-beta-glucosidase 12 isoform X2 [Cucumis sativus][more]
XP_038900662.11.9e-19890.61glucan endo-1,3-beta-glucosidase 1 isoform X2 [Benincasa hispida][more]
KAA0045782.12.4e-19695.15glucan endo-1,3-beta-glucosidase 13 [Cucumis melo var. makuwa] >TYJ99499.1 gluca... [more]
Match NameE-valueIdentityDescription
AT5G67460.14.6e-7343.77O-Glycosyl hydrolases family 17 protein [more]
AT1G79480.13.4e-3144.64Carbohydrate-binding X8 domain superfamily protein [more]
AT1G79480.23.4e-3144.64Carbohydrate-binding X8 domain superfamily protein [more]
AT4G26830.17.8e-2826.13O-Glycosyl hydrolases family 17 protein [more]
AT2G05790.14.7e-2525.78O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 309..394
e-value: 1.3E-49
score: 180.8
IPR012946X8 domainPFAMPF07983X8coord: 309..381
e-value: 7.9E-23
score: 80.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..192
e-value: 4.0E-20
score: 74.0
NoneNo IPR availableGENE3D1.20.58.1040coord: 305..396
e-value: 3.3E-12
score: 48.7
NoneNo IPR availablePANTHERPTHR32227:SF237GB|AAD30228.1coord: 12..394
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 48..172
e-value: 1.2E-8
score: 35.0
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 12..394

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0017526.1PI0017526.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds