PI0017371 (gene) Melon (PI 482460) v1

Overview
NamePI0017371
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCytochrome P450, putative
Locationchr06: 16497731 .. 16501987 (-)
RNA-Seq ExpressionPI0017371
SyntenyPI0017371
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCTCTCTCCTATATTTCTCTCTCTCTCAAAAACTTTCCTCTCAACTCTGCCATGGCGATTATGATTTTTCTAACAACACTTTTGGTGATTTTTATTTCATCACTTGCAAAGATTGCATATGATACAATCTCATGCTACTGGTTGACCCCAAGAAGAATCAGGAAGAAGATGGAGAAACAGGGTGTTCGAGGCCCCTACCCTCGCCCGCTTATAGGAAACATCCTTGATGTAGCAGCCCTTGTAGCAAAATCCACCGCCAAGGACATGAGCTCCATCGATCACAACATCGTCGACCGCCTATTGCCTCATTACACCACCTGGACAAAGCAATACGGTACGCCATGCATATACCATCAATAATAAAAAAACAAAATTGAAAAAAAAAAAATGGTTAGAGATTCGAATCTGTCAAGCGAACTGAATTATATTGGTAAACATTAAAAAACAAACATGGTTTCTTTTAAGATGTGACTTTTTGAACATGCATGATTGAATAATATATATTATAACCTATCAAGTTAAGCTTTTGAGTTAGGAATTTTATGGTACAGGAAAGAGGTTTATATATTGGAACGGGATGGAGCCGAGGCTATGTTTGGCAGAGACGGAATTGATAAAGAAGTATTGAGTAAAAACAGCAATGTGTGTGGGAGATCATGGCTGCAAAGACAGGGAACCAAACATTTCATTGGGAAAGGATTGTTGATGGCAAATGGAGAAGATTGGTATCATCAACGACACATCGTTGCCCCTTCGTTTATGGGGGATAAACTCAAGGTAACTCACTCTATAACCGAATATAACAAATAATATTTATATATATACATATTTATGAAGAAATGGAAAATAGTTTGTCGGACAACAGACTGTGATGCAAAAACACCCAAATATTTATCTAATTGAATTTTATGCTTTCAAATTTATCTTCCTTTTATCTCCTTTATCTTGTTTAATTTGGTAAGTTTATGTTTTCCATTTTTTTCTACTTTTTTATCATTAGAAATAGAGGCAAATATGGAAAGAGGTTTGATCATTATTATTATTTTGATATATTTTGAATTGAAAAAAAGTGTAGACGTTTGTGTTTTGGTAAATGTTTGATGTTTGCTATTTAAAATTAGCGTTTTAGTACAACAATTGGAAATGGTTAAATTTTTTTTATATCCCTATCTTTATATTATACTAAAACAATCGGGGTTAATTCATAAATTATATGATCTAACAAAAACGTAGGTAGATATTGTTAGAGTTATTTCTAAAAAATGAAAGTAAAATGTAGAAATAGATGGAAGAAGAAGATACCGTACAGAAAGAAAGAATGACGGTTGAATATAATTGAGAAAGTTGACATAAAAATTGTTCCGTTGCCTTTTTGTTGGTTAAAAAGAGAGAAGATAGGACCCATTATGTAATATATGTCTAAAAGCATATAAATTAATTTATGGGACAGGGAATAAATTTGATTTTATCATATGTTTAATAATTTGTAATTATATAATTGGAATTGTTATATGATTATTAATTGTAATGACATGAAAAGAGCTATGCGGGGTTGATGGTGGAATGCACTAACAAATTGATCCAATCACTTGAGACCGAGTTTGACTCGGGCCGGACCGAGTTTGAGATCGCCGATTATATGAAACAACTCACCGCCGACATCATTTCTCGGACCGAGTTCGATGCTAATTCCGACAAGGGCAACCAAATCTTTCATTTGCTTACTCTTCTTCAGCACCTCTGTGCCCAAGCCAGCCGCCACCTCTGCCTTCCCGGTAGCAGGTAACCTTTCTTTATGTCAACTTCCAATTCCTATACCCATTTTTTTTGTCTAAAAATTTCGTTATTTTTTCTCTCTCTTTTTTAAATCTAATAATTTAGATATTAAAAACATTTATTAGTTAAAATATCATTTTAATTCCTATGGAATTTTTACTTTATCCTTCATTTTCTTTTTATTTGTTTACTTTAAGTTCCTAGTCAAATTACAAATTAAAAAAAAAAATGTTAATTAAACATTACTTTTACTTTATATAACTTTTTGTAATCTCAGACAACTAAAGTGGATAACTCGGACTCTTTCCTCCCTGGGCTCTTGTTCACACCCTCACATTTATTGAATTTAAAAGTTTATATTGATTTTGTTTAATAATATGACATCCTAAAACAATTTCAACTATAAATCACTTAGTTGCTAACACATATATACTGTTAGATCCATGTTCTATTCTGCAAATTATATTCATAAAAACACTTTCACAACTTTCATCAATATCTCTACTTTATATAATATAAAAACAAATTGAATTAGTTTATAGGTCATTGTTCTTAATAACAAAACTGTTAGAATATATTCACAAAACATAATAAAATTTCAAATTGTATTAGAAACTGAATTTGAACTCTATAGTATTATCCATGATAAATTTGAAATTTTTAAATATTTTTTAAATATTTTAGATTATTTTGCTATATTTGAAAATGATAGATAGTTTATCTCCAAATATATATTTGTAAAAAAATTGTCCGACTATTCTCTGTCATTATAATATTGCTTTGGAGGTGGGCAATGGAGTTTTAGTGTTTATAATTTAATTAATTTTTGTGCAGGTTTTTTCCAAGTAAATACAACAGGGAAATAAAGTCACTAAAAATGGAGGTAGAGAGGTTATTAATGGAGATTATTCAAAGCAGAAAAGATGGTGTGGAGATTGGAAGAAGTAATTGCTATGGAAGTGATTTATTGGGAATGCTATTGAATGAAATGCAAAGGAAAAGAGATGTTGGGGATAATGGTTTCAGCTTGAACTTGCAACTCATAATGGATGAATGCAAAACATTCTTCTTTGCTGGACATGAAACCACTGCCCTTTTGCTCACTTGGACCATCATGTTGCTTGCCACTAGCCCTAATTGGCAACAAAAAGTTCGTGATGAAGTCAATCAAGTTTGCCATAATGGTGCTACTCCCTCTGTTGAACATCTCTCTAAATTCACTTTGGTAAGCAAACTACAATTTTCTCGCCCCGTCACTTGTCCCTATTCCCATCTTGCACTTCAACTTTAGGGTTTAGTGGTAATTTAATCACGAGTTGAATTATAATATATTCGCAAGAATATACTGATTGCAACACGTTAAATTGTTAAAGGGTGTTTGGAACACCAAGTTGGTTATTATAGTATGTTGATCATAATAGTTCGTAAGTTCCAAACATTTTCCATTATGACCTACAAAATTATAATTCACAACTATAATGATCAAATCGGTAGTATGTGTTTGAAGTGTATATTATTTAAGTTGGGTGGGAAATAGTAACCACTAAAAAAGAGAAAAGGGGCGGGAAAGTAGAAAATAGTAAACATATAATACTGCAGTTAATTGTATGTTAATAGTTGGAAATACCAACTACTATAATTTGTTTTGAACAAGACGTGAGAGGTCGAGGGATCAGTATATGCATCAAGATATAATAGTTGAAAACACCAACTACTATAATTAGAGCCTCAAATATAGAGTGAGCTATAATATAATGATCACTCACCCACTTAGAAGTTGAATTCTCAAACAAATTAAATGGTTAGATATATGATCATATATTTGGTTGAATTTTCAAAAAGTAATTTCTTGTTAACCTAGCTATATGATCTCACATTAATTAGTTTCGAGACAGAACATGCTTAACTAACATGAATGAAGTTTAAACGGGTAATTAATGCGTATTTTTCAGCTAAACATGGTGATAAACGAATCGCTAAGGCTATACCCACCGGCAACAGTGCTACCGAGAATGGCGTTTGAGGACATAAAGGTAGGAGATTTAGAGATCCCAAAAGGTTTATCAATATGGATTCCAGTGCTAGCAATCCATCATAGTGAAGAGTTGTGGGGAAAAGATGTAAACGAGTTCAAACCAGAAAGATTTGCATCAAAGCCATTTAGTGGTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGCCAAGGCTTCGCAATGATGGAAGCTAAGATCATCTTGGCTATGCTTCTCTCTAAGTTTAGTTTCACTATCTCAGATTCTTATCGCCATGCTCCTGTTAGTGTTCTCACAATACAGCCTAAGTATGGAGTTCAAGTTTATTTGACTCCAGTCAATTCTTATCAACCCTAAACCCCTTTTTATTAACTTCTTCTCTTCCTTAATACAAAATATTGTATTTTAGACATATATAAAATGGTACGAAATTGGTGAAATTCCAACTTCCTTA

mRNA sequence

CTCCCTCTCTCCTATATTTCTCTCTCTCTCAAAAACTTTCCTCTCAACTCTGCCATGGCGATTATGATTTTTCTAACAACACTTTTGGTGATTTTTATTTCATCACTTGCAAAGATTGCATATGATACAATCTCATGCTACTGGTTGACCCCAAGAAGAATCAGGAAGAAGATGGAGAAACAGGGTGTTCGAGGCCCCTACCCTCGCCCGCTTATAGGAAACATCCTTGATGTAGCAGCCCTTGTAGCAAAATCCACCGCCAAGGACATGAGCTCCATCGATCACAACATCGTCGACCGCCTATTGCCTCATTACACCACCTGGACAAAGCAATACGGAAAGAGGTTTATATATTGGAACGGGATGGAGCCGAGGCTATGTTTGGCAGAGACGGAATTGATAAAGAAGTATTGAGTAAAAACAGCAATGTGTGTGGGAGATCATGGCTGCAAAGACAGGGAACCAAACATTTCATTGGGAAAGGATTGTTGATGGCAAATGGAGAAGATTGGTATCATCAACGACACATCGTTGCCCCTTCGTTTATGGGGGATAAACTCAAGAGCTATGCGGGGTTGATGGTGGAATGCACTAACAAATTGATCCAATCACTTGAGACCGAGTTTGACTCGGGCCGGACCGAGTTTGAGATCGCCGATTATATGAAACAACTCACCGCCGACATCATTTCTCGGACCGAGTTCGATGCTAATTCCGACAAGGGCAACCAAATCTTTCATTTGCTTACTCTTCTTCAGCACCTCTGTGCCCAAGCCAGCCGCCACCTCTGCCTTCCCGGTAGCAGGTTTTTTCCAAGTAAATACAACAGGGAAATAAAGTCACTAAAAATGGAGGTAGAGAGGTTATTAATGGAGATTATTCAAAGCAGAAAAGATGGTGTGGAGATTGGAAGAAGTAATTGCTATGGAAGTGATTTATTGGGAATGCTATTGAATGAAATGCAAAGGAAAAGAGATGTTGGGGATAATGGTTTCAGCTTGAACTTGCAACTCATAATGGATGAATGCAAAACATTCTTCTTTGCTGGACATGAAACCACTGCCCTTTTGCTCACTTGGACCATCATGTTGCTTGCCACTAGCCCTAATTGGCAACAAAAAGTTCGTGATGAAGTCAATCAAGTTTGCCATAATGGTGCTACTCCCTCTGTTGAACATCTCTCTAAATTCACTTTGCTAAACATGGTGATAAACGAATCGCTAAGGCTATACCCACCGGCAACAGTGCTACCGAGAATGGCGTTTGAGGACATAAAGGTAGGAGATTTAGAGATCCCAAAAGGTTTATCAATATGGATTCCAGTGCTAGCAATCCATCATAGTGAAGAGTTGTGGGGAAAAGATGTAAACGAGTTCAAACCAGAAAGATTTGCATCAAAGCCATTTAGTGGTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGCCAAGGCTTCGCAATGATGGAAGCTAAGATCATCTTGGCTATGCTTCTCTCTAAGTTTAGTTTCACTATCTCAGATTCTTATCGCCATGCTCCTGTTAGTGTTCTCACAATACAGCCTAAGTATGGAGTTCAAGTTTATTTGACTCCAGTCAATTCTTATCAACCCTAAACCCCTTTTTATTAACTTCTTCTCTTCCTTAATACAAAATATTGTATTTTAGACATATATAAAATGGTACGAAATTGGTGAAATTCCAACTTCCTTA

Coding sequence (CDS)

ATGTTTGGCAGAGACGGAATTGATAAAGAAGTATTGAGTAAAAACAGCAATGTGTGTGGGAGATCATGGCTGCAAAGACAGGGAACCAAACATTTCATTGGGAAAGGATTGTTGATGGCAAATGGAGAAGATTGGTATCATCAACGACACATCGTTGCCCCTTCGTTTATGGGGGATAAACTCAAGAGCTATGCGGGGTTGATGGTGGAATGCACTAACAAATTGATCCAATCACTTGAGACCGAGTTTGACTCGGGCCGGACCGAGTTTGAGATCGCCGATTATATGAAACAACTCACCGCCGACATCATTTCTCGGACCGAGTTCGATGCTAATTCCGACAAGGGCAACCAAATCTTTCATTTGCTTACTCTTCTTCAGCACCTCTGTGCCCAAGCCAGCCGCCACCTCTGCCTTCCCGGTAGCAGGTTTTTTCCAAGTAAATACAACAGGGAAATAAAGTCACTAAAAATGGAGGTAGAGAGGTTATTAATGGAGATTATTCAAAGCAGAAAAGATGGTGTGGAGATTGGAAGAAGTAATTGCTATGGAAGTGATTTATTGGGAATGCTATTGAATGAAATGCAAAGGAAAAGAGATGTTGGGGATAATGGTTTCAGCTTGAACTTGCAACTCATAATGGATGAATGCAAAACATTCTTCTTTGCTGGACATGAAACCACTGCCCTTTTGCTCACTTGGACCATCATGTTGCTTGCCACTAGCCCTAATTGGCAACAAAAAGTTCGTGATGAAGTCAATCAAGTTTGCCATAATGGTGCTACTCCCTCTGTTGAACATCTCTCTAAATTCACTTTGCTAAACATGGTGATAAACGAATCGCTAAGGCTATACCCACCGGCAACAGTGCTACCGAGAATGGCGTTTGAGGACATAAAGGTAGGAGATTTAGAGATCCCAAAAGGTTTATCAATATGGATTCCAGTGCTAGCAATCCATCATAGTGAAGAGTTGTGGGGAAAAGATGTAAACGAGTTCAAACCAGAAAGATTTGCATCAAAGCCATTTAGTGGTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGCCAAGGCTTCGCAATGATGGAAGCTAAGATCATCTTGGCTATGCTTCTCTCTAAGTTTAGTTTCACTATCTCAGATTCTTATCGCCATGCTCCTGTTAGTGTTCTCACAATACAGCCTAAGTATGGAGTTCAAGTTTATTTGACTCCAGTCAATTCTTATCAACCCTAA

Protein sequence

MFGRDGIDKEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLLGMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQKVRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEAKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP
Homology
BLAST of PI0017371 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 4.5e-164
Identity = 282/405 (69.63%), Postives = 335/405 (82.72%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L K++ V GRSWLQ+QGTK+FIG+GLLMANG+DW+HQRH+ AP+F G++LK YA  M
Sbjct: 116 KELLMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHM 175

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT+KL++ L  E   G  E EI + M +LTADIISRT+F ++ +KG ++F+ LT+LQ 
Sbjct: 176 VECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQR 235

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
            CAQA+RHLC PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRS+ +G DLL
Sbjct: 236 RCAQATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 295

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           G+LLNEM    D  +N  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA +P WQ+K
Sbjct: 296 GLLLNEMD--IDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEK 355

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR+EV +V      PSV+ LSK T L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPK
Sbjct: 356 VREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPK 415

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFAMME 368
           GLSIWIPVLAIHHSEELWGKD N+F PERF  +PF+ GR FIPFAAGPRNCIGQ FA+ME
Sbjct: 416 GLSIWIPVLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALME 475

Query: 369 AKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNS 413
           AKIILA L+SKF+FTIS +YRHAP+ VLTI+PKYGVQV L P+ S
Sbjct: 476 AKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of PI0017371 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 3.5e-161
Identity = 280/404 (69.31%), Postives = 328/404 (81.19%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L+K++ V G+SWLQ+QGTK FIG+GLLMANGE W+HQRH+ AP+F  D+LK YA  M
Sbjct: 117 KELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT  + + L  E      E EI + M++LTADIISRTEF ++ DKG ++F LLT+LQ 
Sbjct: 177 VECTKMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQR 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQA+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRS+ YG DLL
Sbjct: 237 LCAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           G+LLN+M   ++      +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA +P WQ  
Sbjct: 297 GLLLNQMDSNKN------NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDN 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VRDEV QVC     PSVE LS  T LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPK
Sbjct: 357 VRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFAMME 368
           GLSIWIPVLAIHHS ELWG+D NEF PERF ++ F+  R F+PFAAGPRNCIGQ FAMME
Sbjct: 417 GLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMME 476

Query: 369 AKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVN 412
           AKIILAML+SKFSF IS++YRHAP+ VLTI+PKYGVQ+ L P++
Sbjct: 477 AKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of PI0017371 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.9e-77
Identity = 160/389 (41.13%), Postives = 226/389 (58.10%), Query Frame = 0

Query: 34  GKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECTNKLIQSLETEFDSGRTE--FE 93
           G GL+  NG DW   R I+ P+F  DKLK    LMV+CT ++    + + +   TE    
Sbjct: 196 GNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVL 255

Query: 94  IADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNR 153
           I+   K+LTADII+   F ++  +G ++F     LQ  CA A   L  PG ++ P+  N 
Sbjct: 256 ISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNL 315

Query: 154 EIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLLGMLL-----NEMQRKRDVGDNGF 213
           +I  L M+V   +  II +R        S  YG+DLLG++L     NE ++K  + +   
Sbjct: 316 QIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNESEKKMSIDE--- 375

Query: 214 SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQKVRDEVNQVCHNGATPSVE 273
                 I++ECKTFFFAGHETTA LLTW+ MLL+   +WQ+K+R+EV   C     P  E
Sbjct: 376 ------IIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAE 435

Query: 274 HLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELW 333
             SK  L+N V  ESLRLY P   L R+A ED+K+G+LEIPKG +I +P+  +H  + +W
Sbjct: 436 TCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVW 495

Query: 334 GKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGFAMMEAKIILAMLLSKFSFT 393
           G D ++F P RFA+            + F+ GPR CIGQ FA+MEAK +LAM+L +F   
Sbjct: 496 GSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLN 555

Query: 394 ISDSYRHAPVSVLTIQPKYGVQVYLTPVN 412
           +S  Y+HAP   LT+QP+Y + V L P++
Sbjct: 556 LSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of PI0017371 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 4.1e-77
Identity = 152/410 (37.07%), Postives = 247/410 (60.24%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKH-----FIGKGLLMANGEDWYHQRHIVAPSFMGDKLKS 68
           K+VLSK        + Q Q  +H      +  G+  A G+ W   R ++ P+F  +KLK+
Sbjct: 115 KDVLSK--------FYQFQKPRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKN 174

Query: 69  YAGLMVECTNKLIQSLETEFDS-GRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHL 128
               +   +++++   E    + G+ E ++  Y++ LT+D+ISRT F ++ ++G +IF L
Sbjct: 175 MLPAIYLSSSEIVTKWEEMVSTKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQL 234

Query: 129 LTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNC 188
                 L  QAS+ + LPG RF P+K N+ +K +  EV+  L  II  R   +E G  + 
Sbjct: 235 QREQAELIIQASQTIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSS 294

Query: 189 YGSDLLGMLLNEMQRK-RDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT 248
           +  DLLG+LL    ++ +  G+  F L +  +++ECK FFFAG ETT+ LL WT++LL+ 
Sbjct: 295 H-DDLLGILLESNSKEIKQHGNTNFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQ 354

Query: 249 SPNWQQKVRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKV 308
             +WQ++ ++EV +   N   P  + L+   ++NM++ E LRLYPP   L R  +E+IK+
Sbjct: 355 HQDWQKRAKEEVLRTFGNN-KPDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKL 414

Query: 309 GDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSG--GR--FIPFAAGPRN 368
           G++ +P G+ + +P++ +H+ +E+WG D  EF PERF+        GR  + PF+ GPR 
Sbjct: 415 GEISLPAGVILLLPIILLHYDQEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRI 474

Query: 369 CIGQGFAMMEAKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYL 408
           CIGQ FAM+EAK+ +AM+L +FSF +S SY HAP +++T+QP+YG  + L
Sbjct: 475 CIGQNFAMLEAKMAMAMILQRFSFVLSPSYAHAPHAIITLQPQYGAHLIL 514

BLAST of PI0017371 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.3e-75
Identity = 153/388 (39.43%), Postives = 237/388 (61.08%), Query Frame = 0

Query: 33  IGKGLLMANGEDWYHQRHIVAPSFMGDKLK-SYAGLMVECTN--KLIQSLETEFDSGRTE 92
           I +G+   + + W   R ++ P+F  +KLK       + C+   K  + L +    G  E
Sbjct: 152 ISQGVAAYDKDKWAKHRRLLNPAFHIEKLKHMLPAFHLSCSEMLKKWEKLASASAEGSFE 211

Query: 93  FEIADYMKQLTADIISRTEFDANSDKGNQIFHL-LTLLQHLCAQASRHLCLPGSRFFPSK 152
            ++  Y++ +T+D+ISRT F +N ++G  IF L   +L+H+  Q  +    PG  + P+K
Sbjct: 212 LDVWPYLQDMTSDVISRTAFGSNLEEGKNIFKLQKEILEHI-LQILQSFYFPGMLYLPTK 271

Query: 153 YNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLLGMLL--NEMQRKRDVGDN-- 212
             R +K +  EV   ++ II  R + ++ G  +    DLLG+LL  N  + + D G+N  
Sbjct: 272 RIRRMKKISKEVHSSVLNIIMKRVEAMKAGEPS--NDDLLGILLDSNFKEIENDNGNNSN 331

Query: 213 -GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQKVRDEVNQVCHNGATP 272
               ++ + +++ECK F+FAG ETTA+LL WT++LL+  P WQ + RDEV Q+  N + P
Sbjct: 332 KNCGMSFEEVIEECKLFYFAGQETTAVLLVWTLLLLSKHPEWQMRARDEVLQIFGN-SEP 391

Query: 273 SVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSE 332
             + L+   ++ MVINE LRLYPP  ++ R+  ED K+G L +P G+SI++P + +HH E
Sbjct: 392 DFDALNHLKVITMVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPGGISIFLPTIMLHHDE 451

Query: 333 ELWGKDVNEFKPERFAS--KPFSGGRFI--PFAAGPRNCIGQGFAMMEAKIILAMLLSKF 392
           ELWG D  EFKPERF+      + G+FI  PF+ GPR CIG  FAM+EAK+ LA++L +F
Sbjct: 452 ELWGDDAKEFKPERFSEGISKATKGQFIYFPFSGGPRICIGNNFAMLEAKMALALILQRF 511

Query: 393 SFTISDSYRHAPVSVLTIQPKYGVQVYL 408
           +F IS SY HAP +++T+QP+YG Q+ L
Sbjct: 512 TFEISPSYTHAPHTLVTLQPQYGAQLIL 535

BLAST of PI0017371 vs. ExPASy TrEMBL
Match: A0A5A7V8T6 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold508G00410 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 4.7e-233
Identity = 397/407 (97.54%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM
Sbjct: 78  KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 137

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT+KLIQSLETE DSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH
Sbjct: 138 VECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 197

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 198 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 257

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQRK+D GDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQQK
Sbjct: 258 GMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQK 317

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 318 VRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 377

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNC+GQGFA+MEA
Sbjct: 378 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEA 437

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP 416
           KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTP+NSYQP
Sbjct: 438 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 484

BLAST of PI0017371 vs. ExPASy TrEMBL
Match: A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 4.7e-233
Identity = 397/407 (97.54%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM
Sbjct: 117 KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT+KLIQSLETE DSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH
Sbjct: 177 VECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 237 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQRK+D GDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQQK
Sbjct: 297 GMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQK 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 357 VRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNC+GQGFA+MEA
Sbjct: 417 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEA 476

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP 416
           KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTP+NSYQP
Sbjct: 477 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of PI0017371 vs. ExPASy TrEMBL
Match: A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.0e-232
Identity = 396/407 (97.30%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM
Sbjct: 117 KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFD NSDKGNQIFHLLTLLQH
Sbjct: 177 VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQH 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 237 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQ++R+ GDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQQK
Sbjct: 297 GMLLNEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQK 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 357 VRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFA+MEA
Sbjct: 417 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEA 476

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP 416
           KIILAMLLSKFSF+ISDSYRHAPVSVLTIQPKYGVQVYLTP+NSYQP
Sbjct: 477 KIILAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of PI0017371 vs. ExPASy TrEMBL
Match: A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.4e-200
Identity = 345/404 (85.40%), Postives = 372/404 (92.08%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L K S+VCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAG M
Sbjct: 117 KELLGKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECTNK+IQS+ETE DSGRTEFEIADYMKQLTADIISRTEFD +SDKGNQIFHLLTLLQ 
Sbjct: 177 VECTNKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQR 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLL
Sbjct: 237 LCAQASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQ++++      +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLAT+PNWQ  
Sbjct: 297 GMLLNEMQQRKN--STTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHM 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVC N  TPS++HLSK TLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPK
Sbjct: 357 VRAEVNQVC-NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKD N+F P RFA+K F GGRFIPFA GPRNCIGQGFA+MEA
Sbjct: 417 GLSIWIPVLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEA 476

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNS 413
           KIILAM LSKFSFTISD+YRHAPV VLTIQPK+GVQV LTP +S
Sbjct: 477 KIILAMFLSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of PI0017371 vs. ExPASy TrEMBL
Match: A0A6J1D4G3 (cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111016885 PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.8e-200
Identity = 346/404 (85.64%), Postives = 374/404 (92.57%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L KNS+VCG+SWLQRQGTKHFIGKGLLMANGE WYHQRHI+AP+FMG KLKSYAG M
Sbjct: 28  KELLGKNSSVCGKSWLQRQGTKHFIGKGLLMANGEKWYHQRHILAPAFMGQKLKSYAGYM 87

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT  +IQSL+TE +SGRTEFEIA+YMKQLTADIISRTEFD+N DKG QIFHLLTLLQ 
Sbjct: 88  VECTKNMIQSLQTELNSGRTEFEIAEYMKQLTADIISRTEFDSNYDKGKQIFHLLTLLQR 147

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPS+YNREIKSLKMEVERLLMEII+SRKDGVEIGRS+CYG+DLL
Sbjct: 148 LCAQASRHLCLPGSRFFPSQYNREIKSLKMEVERLLMEIIESRKDGVEIGRSSCYGNDLL 207

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQR +   ++G SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQ K
Sbjct: 208 GMLLNEMQRPK--REDGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHK 267

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           +R ++NQVC NGATP+++ LS+ TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPK
Sbjct: 268 LRAQINQVC-NGATPTLDDLSQLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPK 327

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKD NEF PERFA K  S GRFIPFAAGPRNCIGQ FAMMEA
Sbjct: 328 GLSIWIPVLAIHHSEELWGKDANEFNPERFALKSSSAGRFIPFAAGPRNCIGQAFAMMEA 387

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNS 413
           KIILAM LSKFSFTISD+YRHAPV VLTIQPKYGVQV LTP+NS
Sbjct: 388 KIILAMFLSKFSFTISDTYRHAPVIVLTIQPKYGVQVCLTPLNS 428

BLAST of PI0017371 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 816.6 bits (2108), Expect = 9.7e-233
Identity = 397/407 (97.54%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM
Sbjct: 117 KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT+KLIQSLETE DSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH
Sbjct: 177 VECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 237 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQRK+D GDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQQK
Sbjct: 297 GMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQK 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 357 VRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNC+GQGFA+MEA
Sbjct: 417 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEA 476

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP 416
           KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTP+NSYQP
Sbjct: 477 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of PI0017371 vs. NCBI nr
Match: KAA0063407.1 (cytokinin hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 816.6 bits (2108), Expect = 9.7e-233
Identity = 397/407 (97.54%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM
Sbjct: 78  KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 137

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT+KLIQSLETE DSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH
Sbjct: 138 VECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 197

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 198 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 257

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQRK+D GDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQQK
Sbjct: 258 GMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQK 317

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 318 VRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 377

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNC+GQGFA+MEA
Sbjct: 378 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEA 437

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP 416
           KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTP+NSYQP
Sbjct: 438 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 484

BLAST of PI0017371 vs. NCBI nr
Match: XP_004145660.1 (cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009800 [Cucumis sativus])

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-232
Identity = 396/407 (97.30%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM
Sbjct: 117 KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFD NSDKGNQIFHLLTLLQH
Sbjct: 177 VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQH 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 237 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQ++R+ GDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQQK
Sbjct: 297 GMLLNEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQK 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 357 VRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFA+MEA
Sbjct: 417 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEA 476

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQP 416
           KIILAMLLSKFSF+ISDSYRHAPVSVLTIQPKYGVQVYLTP+NSYQP
Sbjct: 477 KIILAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of PI0017371 vs. NCBI nr
Match: XP_038905687.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 776.9 bits (2005), Expect = 8.5e-221
Identity = 378/404 (93.56%), Postives = 392/404 (97.03%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KEVLSKNSNVCG+SWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAGLM
Sbjct: 117 KEVLSKNSNVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGLM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT K+IQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKG +IFHLLTLLQ 
Sbjct: 177 VECTKKMIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQR 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRS CYGSDLL
Sbjct: 237 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQRKR+  DNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+P+WQ K
Sbjct: 297 GMLLNEMQRKRE--DNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPDWQHK 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR EVNQVC+NG +PSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK
Sbjct: 357 VRHEVNQVCNNGGSPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWG+D NEFKPERFASKPFSGGRFIPFA+GPRNCIGQGFA+MEA
Sbjct: 417 GLSIWIPVLAIHHSEELWGEDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEA 476

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNS 413
           KIILAM +SKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTP+NS
Sbjct: 477 KIILAMFISKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 518

BLAST of PI0017371 vs. NCBI nr
Match: KAG6581177.1 (Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 709.9 bits (1831), Expect = 1.3e-200
Identity = 342/404 (84.65%), Postives = 369/404 (91.34%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L K S+VCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAP+FMGDKLKSYAG M
Sbjct: 78  KELLGKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFM 137

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECTNK+IQS+ETE +SGRTEFEIADYMKQLTADIISRTEFD +SDKGNQIFHLLTLLQ 
Sbjct: 138 VECTNKMIQSMETELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQR 197

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLL
Sbjct: 198 LCAQASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLL 257

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           GMLLNEMQ+++++     +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLAT+PNWQ  
Sbjct: 258 GMLLNEMQQRKNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHM 317

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR +VNQVC N   PS++HLSK TLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPK
Sbjct: 318 VRAQVNQVC-NARAPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPK 377

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFAMMEA 368
           GLSIWIPVLAIHHSEELWGKD N+F P RF +K F GGRFIPFA GPRNCIGQGFA+MEA
Sbjct: 378 GLSIWIPVLAIHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEA 437

Query: 369 KIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNS 413
           KIILAM LSKF FTISD+YRHAPV VLTIQPKYGVQV LTP  S
Sbjct: 438 KIILAMFLSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 480

BLAST of PI0017371 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 578.9 bits (1491), Expect = 3.2e-165
Identity = 282/405 (69.63%), Postives = 335/405 (82.72%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L K++ V GRSWLQ+QGTK+FIG+GLLMANG+DW+HQRH+ AP+F G++LK YA  M
Sbjct: 116 KELLMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHM 175

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT+KL++ L  E   G  E EI + M +LTADIISRT+F ++ +KG ++F+ LT+LQ 
Sbjct: 176 VECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQR 235

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
            CAQA+RHLC PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRS+ +G DLL
Sbjct: 236 RCAQATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 295

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           G+LLNEM    D  +N  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA +P WQ+K
Sbjct: 296 GLLLNEMD--IDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEK 355

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VR+EV +V      PSV+ LSK T L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPK
Sbjct: 356 VREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPK 415

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFAMME 368
           GLSIWIPVLAIHHSEELWGKD N+F PERF  +PF+ GR FIPFAAGPRNCIGQ FA+ME
Sbjct: 416 GLSIWIPVLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALME 475

Query: 369 AKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVNS 413
           AKIILA L+SKF+FTIS +YRHAP+ VLTI+PKYGVQV L P+ S
Sbjct: 476 AKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of PI0017371 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 569.3 bits (1466), Expect = 2.5e-162
Identity = 280/404 (69.31%), Postives = 328/404 (81.19%), Query Frame = 0

Query: 9   KEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLM 68
           KE+L+K++ V G+SWLQ+QGTK FIG+GLLMANGE W+HQRH+ AP+F  D+LK YA  M
Sbjct: 117 KELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHM 176

Query: 69  VECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQH 128
           VECT  + + L  E      E EI + M++LTADIISRTEF ++ DKG ++F LLT+LQ 
Sbjct: 177 VECTKMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQR 236

Query: 129 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLL 188
           LCAQA+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRS+ YG DLL
Sbjct: 237 LCAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLL 296

Query: 189 GMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQK 248
           G+LLN+M   ++      +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA +P WQ  
Sbjct: 297 GLLLNQMDSNKN------NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDN 356

Query: 249 VRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 308
           VRDEV QVC     PSVE LS  T LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPK
Sbjct: 357 VRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPK 416

Query: 309 GLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFAMME 368
           GLSIWIPVLAIHHS ELWG+D NEF PERF ++ F+  R F+PFAAGPRNCIGQ FAMME
Sbjct: 417 GLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMME 476

Query: 369 AKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPVN 412
           AKIILAML+SKFSF IS++YRHAP+ VLTI+PKYGVQ+ L P++
Sbjct: 477 AKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of PI0017371 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 291.2 bits (744), Expect = 1.3e-78
Identity = 160/389 (41.13%), Postives = 226/389 (58.10%), Query Frame = 0

Query: 34  GKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECTNKLIQSLETEFDSGRTE--FE 93
           G GL+  NG DW   R I+ P+F  DKLK    LMV+CT ++    + + +   TE    
Sbjct: 196 GNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVL 255

Query: 94  IADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNR 153
           I+   K+LTADII+   F ++  +G ++F     LQ  CA A   L  PG ++ P+  N 
Sbjct: 256 ISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNL 315

Query: 154 EIKSLKMEVERLLMEIIQSRKDGVEIGRSNCYGSDLLGMLL-----NEMQRKRDVGDNGF 213
           +I  L M+V   +  II +R        S  YG+DLLG++L     NE ++K  + +   
Sbjct: 316 QIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNESEKKMSIDE--- 375

Query: 214 SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQKVRDEVNQVCHNGATPSVE 273
                 I++ECKTFFFAGHETTA LLTW+ MLL+   +WQ+K+R+EV   C     P  E
Sbjct: 376 ------IIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAE 435

Query: 274 HLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELW 333
             SK  L+N V  ESLRLY P   L R+A ED+K+G+LEIPKG +I +P+  +H  + +W
Sbjct: 436 TCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVW 495

Query: 334 GKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGFAMMEAKIILAMLLSKFSFT 393
           G D ++F P RFA+            + F+ GPR CIGQ FA+MEAK +LAM+L +F   
Sbjct: 496 GSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLN 555

Query: 394 ISDSYRHAPVSVLTIQPKYGVQVYLTPVN 412
           +S  Y+HAP   LT+QP+Y + V L P++
Sbjct: 556 LSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of PI0017371 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 286.6 bits (732), Expect = 3.2e-77
Identity = 154/412 (37.38%), Postives = 228/412 (55.34%), Query Frame = 0

Query: 8   DKEVLS-KNSNVCGRSW----LQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLK 67
           D E LS  +  V G+SW    + ++  +   G GL+M  G+DW   RHI+ P+F    LK
Sbjct: 119 DPEFLSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLK 178

Query: 68  SYAGLMVECTNKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHL 127
               +MVE  + ++     + +SG  EF++   +     +II++T F    + G Q+   
Sbjct: 179 VMTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKN 238

Query: 128 LTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSNC 187
           L  +Q     ++R++ +P S     K   + K L  E++ LL+  I  RK  + +   + 
Sbjct: 239 LRAVQFALFNSNRYVGVPFSNILSYKQTVKAKGLGHEIDGLLLSFINKRK--ISLAEGDD 298

Query: 188 YGSDLLGMLLNEMQRKRDVGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATS 247
            G DLLGMLL          D   +   + ++DECKTFFFAGHETTAL LTWT MLLA  
Sbjct: 299 QGHDLLGMLLK--------ADQKGNFTAKELVDECKTFFFAGHETTALALTWTFMLLAIH 358

Query: 248 PNWQQKVRDEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVG 307
           P WQ  +R+E+ +V  +        L+    ++ V+NE LRLYPPA    R A  DI+V 
Sbjct: 359 PEWQDTIREEIREVIGDSKI-EYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVN 418

Query: 308 DLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNC 367
              IP G +IWI V+A+HH  ELWG DVNEFKPERF      G +    ++PF  G R C
Sbjct: 419 GRVIPNGTNIWIDVVAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMC 478

Query: 368 IGQGFAMMEAKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPV 411
           IG+    ME KI+L+++LS+F  ++S  YRH+P  +L+++P YG+ + + P+
Sbjct: 479 IGRNLTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of PI0017371 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.6e-76
Identity = 142/394 (36.04%), Postives = 233/394 (59.14%), Query Frame = 0

Query: 30  KHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECTNKLIQSLETEF-DSGRT 89
           K   G GLL   GE W H R I++P+F  + LK    ++++    ++     +  ++G  
Sbjct: 137 KQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEV 196

Query: 90  EFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSK 149
           E ++ ++ + LT D+ISRT F ++ + G  +F L      LCA+A + + +PG RFFP++
Sbjct: 197 EVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTR 256

Query: 150 YNREIKSLKMEVERLLMEIIQSRKDGVEIGR-SNC---YGSDLLGMLLNEMQRKRDVGDN 209
            N +   L  E+ + L+++I+ R+     G    C      DLLG+++   Q K      
Sbjct: 257 GNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---QAK------ 316

Query: 210 GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATSPNWQQKVRDEVNQVCHNGATPS 269
             ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+  P WQ K RDEV +VC +   P+
Sbjct: 317 --NVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPT 376

Query: 270 VEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEE 329
            +H+ K   L+M++NESLRLYPP     R A  D+K+G  +IP G  + IP++A+HH + 
Sbjct: 377 KDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQA 436

Query: 330 LWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGFAMMEAKIILAMLLSKFS 389
           +WG DVNEF P RFA       +    FIPF  G R CIGQ  A+++AK+ LA+++ +F+
Sbjct: 437 IWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFT 496

Query: 390 FTISDSYRHAPVSVLTIQPKYGVQVYLTPVNSYQ 415
           F ++ +Y+HAP  ++ + P++G  +    + +++
Sbjct: 497 FHLAPTYQHAPTVLMLLYPQHGAPITFRRLTNHE 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF184.5e-16469.63Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q9ZW953.5e-16169.31Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
F4IK451.9e-7741.13Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
H2DH214.1e-7737.07Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
W8JWW31.3e-7539.43Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V8T64.7e-23397.54Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3CGC04.7e-23397.54cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1[more]
A0A0A0L6Y61.0e-23297.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1[more]
A0A6J1J2Y11.4e-20085.40cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1[more]
A0A6J1D4G31.8e-20085.64cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111016885 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_008461587.19.7e-23397.54PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
KAA0063407.19.7e-23397.54cytokinin hydroxylase-like [Cucumis melo var. makuwa][more]
XP_004145660.12.2e-23297.30cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009... [more]
XP_038905687.18.5e-22193.56cytokinin hydroxylase-like [Benincasa hispida][more]
KAG6581177.11.3e-20084.65Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G38450.13.2e-16569.63cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.12.5e-16269.31cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT2G46950.11.3e-7841.13cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT5G52400.13.2e-7737.38cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
AT2G26710.13.6e-7636.04Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 153..173
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 9..406
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 9..406
IPR001128Cytochrome P450PRINTSPR00385P450coord: 358..369
score: 44.68
coord: 223..240
score: 40.88
coord: 276..287
score: 46.75
coord: 349..358
score: 57.09
IPR001128Cytochrome P450PFAMPF00067p450coord: 9..385
e-value: 7.0E-81
score: 272.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 212..229
score: 29.73
coord: 232..258
score: 28.04
coord: 358..381
score: 34.31
coord: 316..340
score: 24.79
coord: 348..358
score: 52.03
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 7..413
e-value: 4.5E-101
score: 340.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 9..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0017371.1PI0017371.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen