PI0016777 (gene) Melon (PI 482460) v1

Overview
NamePI0016777
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionUbiquitin-conjugating enzyme E2 28
Locationchr01: 5266126 .. 5267466 (-)
RNA-Seq ExpressionPI0016777
SyntenyPI0016777
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCAAGAGAATATCGAAGGAGCTCAAAGACTTGCAGAGAGACCCACCAACTTCTTGCAGTGCAGGTGATTAGTTAATGCAACTTTGACTAGTTTGATAATGTTCTATGTCTGTAGTTACTTTTTTCTTGTTTCCAGTTTTTGACAAATATGAACCCTTTAATATGGTAATACATATTCTCATTTTAGGTCTTGTTTGATAATTATTTTGTTTTTTTTTTCCTTTTTGAGATAAGGATGAAAATCAAACCTCCACCTTGATACAGTACTGGATGAGGACCAAGAATTCACCTGTTTAATCTTTTTTTTTTCTAATTTTGGGAAAATTTTCTGTATGCAAATCAAATGTGAGTTTGTGTTATATATATGGTGCATTTCTTCAGGTCCTGTAGCAGAGGATATGTTCCATTGGCAAGCAACCATTATTGGTCCCAGTGATAGTCCCTATTCTGGTGGAGTTTTTCTTGTTACCATTCATTTTCCTCCTGATTATCCTTTCAAACCTCCTAAGGTAAGCCATGCTATGCCTTTTCACTTCAACGGCTAAATGCTTGATGAGTTAAATTTTGATTAAGTTGAAGGAATTATTGGTCATTATTTGGATCCTTTCGGATTCACTAGACAAAAAAATGTATTCCAAAAAAAAGACACTTTTATTTAAATTCTTTTGATAAAAAGTGTGAAATCACTTGTTTTCGAAATTAAACATTTAAAATAGTTAACTAATTAGACACCCTTAATTTCAGGTTGCTTTTAGGACAAAGGTGTTTCATCCTAACATAAACAGTAATGGCAACATATGCTTGGATATCCTCAAGGAACAATGGAGTCCTGCCCTCACAATTTCAAAGGTAACATTTTCTTCAAACTCTAGTTGAACAGTAAGAGAGTAATTTAAGAAGACGTATGTTCTAAAAATTGTGAACTCTTTCTATCTTTTTGATGTTGGTTCACATTTTGTGTCAGATTCGATATGAGATTTTTAATATCTCCCTCGAGATGATGTGCTATTTCGATTCATCATTCTTGGGTCATGTCCCAAAAGTTTTTAAGTCTTCCCTCCATCTTTTCGACGTTGAATTCTCTAACAAATTTTTAGTTTTTAACTCGAAATCGAGTTTGTTGAGCACATACATTTACAAAACCATGTTTATATTAATTAATTAATTTCATTTTGGTTTTGGTTTTTGTAGGTTTTGCTCTCAATCTGCTCTCTATTAACAGACCCAAACCCAGATGATCCATTGGTTCCTGAGATTGCTCATATGTGCAAGACTGACAAACTCAAATATGAATCCACTGCTAGAAGCTGGACCCATAAATATGCCATGGGTTAA

mRNA sequence

ATGGCTTCCAAGAGAATATCGAAGGAGCTCAAAGACTTGCAGAGAGACCCACCAACTTCTTGCAGTGCAGGTCCTGTAGCAGAGGATATGTTCCATTGGCAAGCAACCATTATTGGTCCCAGTGATAGTCCCTATTCTGGTGGAGTTTTTCTTGTTACCATTCATTTTCCTCCTGATTATCCTTTCAAACCTCCTAAGGTTGCTTTTAGGACAAAGGTGTTTCATCCTAACATAAACAGTAATGGCAACATATGCTTGGATATCCTCAAGGAACAATGGAGTCCTGCCCTCACAATTTCAAAGGTTTTGCTCTCAATCTGCTCTCTATTAACAGACCCAAACCCAGATGATCCATTGGTTCCTGAGATTGCTCATATGTGCAAGACTGACAAACTCAAATATGAATCCACTGCTAGAAGCTGGACCCATAAATATGCCATGGGTTAA

Coding sequence (CDS)

ATGGCTTCCAAGAGAATATCGAAGGAGCTCAAAGACTTGCAGAGAGACCCACCAACTTCTTGCAGTGCAGGTCCTGTAGCAGAGGATATGTTCCATTGGCAAGCAACCATTATTGGTCCCAGTGATAGTCCCTATTCTGGTGGAGTTTTTCTTGTTACCATTCATTTTCCTCCTGATTATCCTTTCAAACCTCCTAAGGTTGCTTTTAGGACAAAGGTGTTTCATCCTAACATAAACAGTAATGGCAACATATGCTTGGATATCCTCAAGGAACAATGGAGTCCTGCCCTCACAATTTCAAAGGTTTTGCTCTCAATCTGCTCTCTATTAACAGACCCAAACCCAGATGATCCATTGGTTCCTGAGATTGCTCATATGTGCAAGACTGACAAACTCAAATATGAATCCACTGCTAGAAGCTGGACCCATAAATATGCCATGGGTTAA

Protein sequence

MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMCKTDKLKYESTARSWTHKYAMG
Homology
BLAST of PI0016777 vs. ExPASy Swiss-Prot
Match: Q94F47 (Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 9.2e-80
Identity = 139/148 (93.92%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPN+NSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTD+ KYESTARSWT KYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. ExPASy Swiss-Prot
Match: P35132 (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-79
Identity = 140/148 (94.59%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTDK KYESTAR+WT KYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

BLAST of PI0016777 vs. ExPASy Swiss-Prot
Match: P35133 (Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana OX=3702 GN=UBC10 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 3.5e-79
Identity = 139/148 (93.92%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPS+SPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTDK KYESTARSWT KYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. ExPASy Swiss-Prot
Match: P35135 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 2.3e-78
Identity = 136/148 (91.89%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GP+DSPY+GGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTD+ KYE+TARSWT KYAMG
Sbjct: 121 PEIAHMYKTDRAKYETTARSWTQKYAMG 148

BLAST of PI0016777 vs. ExPASy Swiss-Prot
Match: P35131 (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 2.3e-78
Identity = 136/148 (91.89%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GP++SPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTD+ KYE+TAR+WT KYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

BLAST of PI0016777 vs. ExPASy TrEMBL
Match: A0A0A0K4N5 (Ubiquitin carrier protein OS=Cucumis sativus OX=3659 GN=Csa_7G011870 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.0e-81
Identity = 147/148 (99.32%), Postives = 147/148 (99.32%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKLKYESTARSWTHKYAMG
Sbjct: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 148

BLAST of PI0016777 vs. ExPASy TrEMBL
Match: A0A5D3CNZ1 (Ubiquitin-conjugating enzyme E2 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00620 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.3e-81
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKL+YESTARSWTHKYAMG
Sbjct: 121 PEIAHMCKTDKLRYESTARSWTHKYAMG 148

BLAST of PI0016777 vs. ExPASy TrEMBL
Match: A0A1S3BI25 (ubiquitin-conjugating enzyme E2 28 OS=Cucumis melo OX=3656 GN=LOC103490290 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.3e-81
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKL+YESTARSWTHKYAMG
Sbjct: 121 PEIAHMCKTDKLRYESTARSWTHKYAMG 148

BLAST of PI0016777 vs. ExPASy TrEMBL
Match: A0A6J1C6F1 (ubiquitin-conjugating enzyme E2 10-like OS=Momordica charantia OX=3673 GN=LOC111007810 PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.3e-80
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGP+DSPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPNDSPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKLKYESTARSWT KYAMG
Sbjct: 121 PEIAHMCKTDKLKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. ExPASy TrEMBL
Match: M5W8B0 (UBC core domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa012939mg PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.3e-80
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGP+DSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPNDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDK+KYESTARSWT KYAMG
Sbjct: 121 PEIAHMCKTDKVKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. NCBI nr
Match: XP_004144844.1 (ubiquitin-conjugating enzyme E2 28 [Cucumis sativus] >KGN43247.1 hypothetical protein Csa_020408 [Cucumis sativus])

HSP 1 Score: 312.4 bits (799), Expect = 2.1e-81
Identity = 147/148 (99.32%), Postives = 147/148 (99.32%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKLKYESTARSWTHKYAMG
Sbjct: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 148

BLAST of PI0016777 vs. NCBI nr
Match: XP_008447960.1 (PREDICTED: ubiquitin-conjugating enzyme E2 28 [Cucumis melo] >KAA0049752.1 ubiquitin-conjugating enzyme E2 28 [Cucumis melo var. makuwa] >TYK12126.1 ubiquitin-conjugating enzyme E2 28 [Cucumis melo var. makuwa])

HSP 1 Score: 311.2 bits (796), Expect = 4.7e-81
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKL+YESTARSWTHKYAMG
Sbjct: 121 PEIAHMCKTDKLRYESTARSWTHKYAMG 148

BLAST of PI0016777 vs. NCBI nr
Match: XP_007201846.1 (ubiquitin-conjugating enzyme E2 28 isoform X2 [Prunus persica])

HSP 1 Score: 307.0 bits (785), Expect = 8.9e-80
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGP+DSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPNDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDK+KYESTARSWT KYAMG
Sbjct: 121 PEIAHMCKTDKVKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. NCBI nr
Match: ONH92243.1 (hypothetical protein PRUPE_8G165100 [Prunus persica])

HSP 1 Score: 307.0 bits (785), Expect = 8.9e-80
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGP+DSPYSGGVFLVTIHFPPDY
Sbjct: 27  MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPNDSPYSGGVFLVTIHFPPDY 86

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 87  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 146

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDK+KYESTARSWT KYAMG
Sbjct: 147 PEIAHMCKTDKVKYESTARSWTQKYAMG 174

BLAST of PI0016777 vs. NCBI nr
Match: XP_022136003.1 (ubiquitin-conjugating enzyme E2 10-like [Momordica charantia])

HSP 1 Score: 307.0 bits (785), Expect = 8.9e-80
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQRDPPTSCSAGPVAEDMFHWQATIIGP+DSPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPNDSPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHMCKTDKLKYESTARSWT KYAMG
Sbjct: 121 PEIAHMCKTDKLKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 297.4 bits (760), Expect = 6.6e-81
Identity = 139/148 (93.92%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPN+NSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTD+ KYESTARSWT KYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 297.4 bits (760), Expect = 6.6e-81
Identity = 139/148 (93.92%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPN+NSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTD+ KYESTARSWT KYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

BLAST of PI0016777 vs. TAIR 10
Match: AT4G27960.1 (ubiquitin conjugating enzyme 9 )

HSP 1 Score: 297.0 bits (759), Expect = 8.6e-81
Identity = 140/148 (94.59%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPSDSPYSGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTDK KYESTAR+WT KYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

BLAST of PI0016777 vs. TAIR 10
Match: AT4G27960.2 (ubiquitin conjugating enzyme 9 )

HSP 1 Score: 297.0 bits (759), Expect = 8.6e-81
Identity = 140/148 (94.59%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPSDSPYSGGVFLVTIHFPPDY
Sbjct: 31  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 90

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 91  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 150

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTDK KYESTAR+WT KYAMG
Sbjct: 151 PEIAHMYKTDKNKYESTARTWTQKYAMG 178

BLAST of PI0016777 vs. TAIR 10
Match: AT5G53300.1 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 295.4 bits (755), Expect = 2.5e-80
Identity = 139/148 (93.92%), Postives = 144/148 (97.30%), Query Frame = 0

Query: 1   MASKRISKELKDLQRDPPTSCSAGPVAEDMFHWQATIIGPSDSPYSGGVFLVTIHFPPDY 60
           MASKRI KELKDLQ+DPPTSCSAGPVAEDMFHWQATI+GPS+SPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGNICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNG+ICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMCKTDKLKYESTARSWTHKYAMG 149
           PEIAHM KTDK KYESTARSWT KYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94F479.2e-8093.92Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1... [more]
P351321.2e-7994.59SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1[more]
P351333.5e-7993.92Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana OX=3702 GN=UBC10 PE=1... [more]
P351352.3e-7891.89Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
P351312.3e-7891.89Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A0A0K4N51.0e-8199.32Ubiquitin carrier protein OS=Cucumis sativus OX=3659 GN=Csa_7G011870 PE=3 SV=1[more]
A0A5D3CNZ12.3e-8198.65Ubiquitin-conjugating enzyme E2 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BI252.3e-8198.65ubiquitin-conjugating enzyme E2 28 OS=Cucumis melo OX=3656 GN=LOC103490290 PE=3 ... [more]
A0A6J1C6F14.3e-8097.30ubiquitin-conjugating enzyme E2 10-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
M5W8B04.3e-8097.30UBC core domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa012939m... [more]
Match NameE-valueIdentityDescription
XP_004144844.12.1e-8199.32ubiquitin-conjugating enzyme E2 28 [Cucumis sativus] >KGN43247.1 hypothetical pr... [more]
XP_008447960.14.7e-8198.65PREDICTED: ubiquitin-conjugating enzyme E2 28 [Cucumis melo] >KAA0049752.1 ubiqu... [more]
XP_007201846.18.9e-8097.30ubiquitin-conjugating enzyme E2 28 isoform X2 [Prunus persica][more]
ONH92243.18.9e-8097.30hypothetical protein PRUPE_8G165100 [Prunus persica][more]
XP_022136003.18.9e-8097.30ubiquitin-conjugating enzyme E2 10-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G64230.16.6e-8193.92ubiquitin-conjugating enzyme 28 [more]
AT1G64230.26.6e-8193.92ubiquitin-conjugating enzyme 28 [more]
AT4G27960.18.6e-8194.59ubiquitin conjugating enzyme 9 [more]
AT4G27960.28.6e-8194.59ubiquitin conjugating enzyme 9 [more]
AT5G53300.12.5e-8093.92ubiquitin-conjugating enzyme 10 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 4..147
e-value: 2.0E-76
score: 269.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR24068:SF346BNAA01G32320D PROTEINcoord: 3..145
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..145
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..148
e-value: 6.6E-73
score: 245.6
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 2..147
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 5..141
e-value: 3.8E-52
score: 175.7
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..147
score: 59.93235
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 3..142
e-value: 8.06337E-73
score: 212.448
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 74..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016777.1PI0016777.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0007264 small GTPase mediated signal transduction
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity