PI0016716 (gene) Melon (PI 482460) v1

Overview


Name	PI0016716
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr05: 22541289 .. 22544732 (+)
RNA-Seq Expression	PI0016716
Synteny	PI0016716

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCAACCTTTGAAGGGTTTTATGGATGTATGAGTATTTTTTAATCATGAAGAAAAAAGACATAAGTTTTTGAACAGTGAAAGTTAATAAAAAAAAAAGAATAAATTTGAAAAAAGAGATAAAGTAGAGTTAATGCTATGAAATTTTGGAGACGTTGCGTGCGGGCAATGTCAGCTTCTTAGCCTCGGCCTTTGCAAAACCCAATACTGTGTTCTGTTCCCTTTCCTAATAACGACGAGGCGCAGCGCAGCATTCCTTCAATTTTCCATTTTCCATTTTCCATTTCAAAAAGCTTTAACCCATTTCTTTAATTCAATTCAACCAACAATCACATTCACCAGTCGATCCATTTTGAATGGGCTGCCCAGCTAAGCTCTACCAATTTCTTTGACTCTTAACCCTTTTGTTGTTCCAATCTAAGCCCGACACACGCCCATTCCTTCTTCTTTCTCTTTCCATTTCCTTTTGGGGCCAACCATTTCTGCAATTATTGGGTTGTTCAGCGGCGTAAACCTCATTTTTCCACCATTTCCACCTTCCCCTGCTGTTTTCTGGTCGATCACTCTCGTACCCTTCAATTTTTTCCATCTGGGTATGTTCATTTCTTTGCTTACTGATGTTTTATGTTGCTCTACTGTTGATTCTTCAATTGGGTGTTCAAAATTTCACTTGGCTTTTGTTCGATTTCGTAGTTCTTCTATATTTTCTTGCTATACATTGTTTATCACTGTGCTGCATTCTGGTTATGTTTTTGCCTGTTCGATGATTTTGAAACCATGGTGAATTTGTCTGTTGTTTTTCCTTCTTGATTCTTAACTGAAGTGGTTTAGATGGGAAAAATGCTGGCTTCTAGTTTTAAACAGAGATGATTTAATACTCGTTCTATTAATAGATTTTTTGTTGTTTTCTTTTGGTTTTCCTGTATTTTACTTTTCTGTTTATAGATTCTTATCGCAGCACAGATGCTAAACTCAATAAAGAGGAAAAGAGAAAGGAAAAAAATTTGGTGCTTCTGTTGGGTTTTGTTTTCTTGCTGCTTGTACTTGTGCCAATTAATGATATAGCTTTTATTTTCTCACTCTTAAACGCACTTTTTCTGTTGTGCAAGCAAACAATGATTCCATCCAATTGTGACTGTCATGTTGTTGTAAATTTTAATGCATACATGAGTTTTCAATTTAAAGAACCTTGAAGATTCTTCCTCATAGCTTCCATTTCCAACAGGTTCTGTCCTTTCTATGGTATAAGAGAAGGATTTAATGATCTGAAACTTAATTGATTTTACTTCTTGCGCATGCAATGCTTTCAAATTACATCTTGCTTCTCATTTCTCTTGATTTCTGGATGTGAAATTCATTTAGGTATGTAATAAATGCACAGTTATTGTTCTTGATTTGTGATTTAGGAGATCCATGTTTTGAATGTTTTTTTCTATTATTATCCTATTTGCGTTGCACATTTGAAAATAAATATCATTGAAAGTGAAGTTTAAATGGACTCCACATACCTGATATGATCATCCTACTAAATTGATTGGTCCAGGAACAAGTTTTGAGATACTTGCTTATCATAAGTTTCTATAGAACCAATTATGAATTTTTCAAACAAGTCCCTCTAGCAATGACACTTCTTTTTTAATTAAATCTCAGAGATGATTGTATGAGTACCACAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACAGAAGTCAAGGGAAGAACATGCTTCTTCCGCAGCCAGCTTCTGCAAAGAAGTGACTGAGTTGCGCCTTGCATCTATAGATTCTCAACCAGCAACTTCTCGCCCTGAGTTCAAAGGTGTTACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGGTATCATATTCTAATTGTGATGACTTTCAAAACATACAATGCTTGCTCATTGATTATTCTGAAAAAACCGTTTCATAGATAAATAAATCCATCATAAGTAGAACACAAAAGCATTTAATTTGGTTGAAGGCCTAGAACCCTAATGGATAATTGAGTGATCCTTTTGTCCCCGGAAATAGCTTTCAACTAAGTATCAAAAGGATTCTATGTAACTTCACTTTCAGTTCAAATGGAAGGCTAGCAATTTTGTTTAAGTGTTAAATTGCATTATGACTGAGTTATTACATTTGAGGTAACTCCAATGCATACCAGCTAACATAATTTTATGGTTCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACATGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTTACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTAAGATGTCTCTTTGTCTCTTTGCGTCTTTATTTCAAATTCTTAGGAATAATGAAAGCAAAGAAGAAGCAATTTCATGTAATGAACATCCTCCTGATTTCTCATTTGATACAATTCAGGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCCGTTTACATGGCATTTCTCCGGTTCATGGGTGATGACAACGATGCAAAACAATTTTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATCCGCGATAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAGTTGGCATTATTTTGCTCTGGAAATGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGACAACAAATGAATATTCATTTATCAAACTCAAAACTTGTACAATATGTGACATTGTGACCTTTGTACATGCCTCGGCCTTCTACAATTCAGCCCTTTTTAGAACCTCATATTTCTTCGTCACCTACATTTTCTGCTTCATTTTCTTTTCCCTATAATTTATTATTTTACGGAATTATTAAAATAGCATGATAGGAAAAAACTAACAATAATGCCAACCCATTTTCATAGCAATGCAATGCAAAATGTAAAACAAGTCAGCATATTATGTATAGAACTCGAACAATATAAAAAGGTGTTGGACAAGTTTAATAGTAAATGCTGTTGAAGTTTTCATTGTTATGCTAAAACTTT

mRNA sequence

ATTCAACCTTTGAAGGGTTTTATGGATGTATGAGTATTTTTTAATCATGAAGAAAAAAGACATAAGTTTTTGAACAGTGAAAGTTAATAAAAAAAAAAGAATAAATTTGAAAAAAGAGATAAAGTAGAGTTAATGCTATGAAATTTTGGAGACGTTGCGTGCGGGCAATGTCAGCTTCTTAGCCTCGGCCTTTGCAAAACCCAATACTGTGTTCTGTTCCCTTTCCTAATAACGACGAGGCGCAGCGCAGCATTCCTTCAATTTTCCATTTTCCATTTTCCATTTCAAAAAGCTTTAACCCATTTCTTTAATTCAATTCAACCAACAATCACATTCACCAGTCGATCCATTTTGAATGGGCTGCCCAGCTAAGCTCTACCAATTTCTTTGACTCTTAACCCTTTTGTTGTTCCAATCTAAGCCCGACACACGCCCATTCCTTCTTCTTTCTCTTTCCATTTCCTTTTGGGGCCAACCATTTCTGCAATTATTGGGTTGTTCAGCGGCGTAAACCTCATTTTTCCACCATTTCCACCTTCCCCTGCTGTTTTCTGGTCGATCACTCTCGTACCCTTCAATTTTTTCCATCTGGAGATGATTGTATGAGTACCACAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACAGAAGTCAAGGGAAGAACATGCTTCTTCCGCAGCCAGCTTCTGCAAAGAAGTGACTGAGTTGCGCCTTGCATCTATAGATTCTCAACCAGCAACTTCTCGCCCTGAGTTCAAAGGTGTTACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACATGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTTACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCCGTTTACATGGCATTTCTCCGGTTCATGGGTGATGACAACGATGCAAAACAATTTTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATCCGCGATAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAGTTGGCATTATTTTGCTCTGGAAATGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGACAACAAATGAATATTCATTTATCAAACTCAAAACTTGTACAATATGTGACATTGTGACCTTTGTACATGCCTCGGCCTTCTACAATTCAGCCCTTTTTAGAACCTCATATTTCTTCGTCACCTACATTTTCTGCTTCATTTTCTTTTCCCTATAATTTATTATTTTACGGAATTATTAAAATAGCATGATAGGAAAAAACTAACAATAATGCCAACCCATTTTCATAGCAATGCAATGCAAAATGTAAAACAAGTCAGCATATTATGTATAGAACTCGAACAATATAAAAAGGTGTTGGACAAGTTTAATAGTAAATGCTGTTGAAGTTTTCATTGTTATGCTAAAACTTT

Coding sequence (CDS)

ATGAGTACCACAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACAGAAGTCAAGGGAAGAACATGCTTCTTCCGCAGCCAGCTTCTGCAAAGAAGTGACTGAGTTGCGCCTTGCATCTATAGATTCTCAACCAGCAACTTCTCGCCCTGAGTTCAAAGGTGTTACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACATGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTTACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCCGTTTACATGGCATTTCTCCGGTTCATGGGTGATGACAACGATGCAAAACAATTTTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATCCGCGATAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAGTTGGCATTATTTTGCTCTGGAAATGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGA

Protein sequence

MSTTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWRVR

Homology

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BLAST of PI0016716 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.2e-132
Identity = 218/301 (72.43%), Postives = 250/301 (83.06%), Query Frame = 0


Query: 28  SFCKEVTELRLASIDSQPATSRPEFKGVTFRKASTGGFAR----SRNDVHELLDCTVCMN 87
           S  KEV E     +D +  T++ E       K  + G  +    S N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 88  LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGC 147
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 148 IDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHR 207
            D+FPYYSKLKHE++C++RPY CPYAG EC VTGDIP+LV+HLKDDH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 208 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNDAKQFSYS 267
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+N+AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 268 LEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIW 325
           LEVG +GRKL WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG +  ELKL+VTGRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

[Close]

BLAST of PI0016716 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 7.4e-130
Identity = 216/290 (74.48%), Postives = 248/290 (85.52%), Query Frame = 0


Query: 35  ELRLASIDSQPATSRPEFKGVTFRKASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCAN 94
           E+++A ++   A S+P   G      S G F  S N V+ELL+C VC NLM+PPI+QC N
Sbjct: 22  EVKMAKVE---ANSKPTKSG----SGSIGKF-HSSNGVYELLECPVCTNLMYPPIHQCPN 81

Query: 95  GHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLK 154
           GHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC D+FPYYSKLK
Sbjct: 82  GHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLK 141

Query: 155 HEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVEN 214
           HE++C++R Y+CPYAG EC VTGDIP+LV HLKDDH+VDMHDGC+F+HRYVKSNP EVEN
Sbjct: 142 HEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVEN 201

Query: 215 ATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLI 274
           ATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+N+AK+FSYSLEVG + RKL 
Sbjct: 202 ATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLT 261

Query: 275 WQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG++  ELKL+VTGRIW+
Sbjct: 262 WQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

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BLAST of PI0016716 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 4.6e-124
Identity = 196/255 (76.86%), Postives = 224/255 (87.84%), Query Frame = 0


Query: 70  NDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVA 129
           + V ELL+C VC++ M+PPI+QC+NGHTLCS CK RV N CPTCRHELGNIRCLALEKVA
Sbjct: 45  SSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 104

Query: 130 ESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDD 189
            SLELPC YQN GC+ ++PYY KLKHE  C+YRPY+CPYAG EC V GDIP LV HLKDD
Sbjct: 105 ASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDD 164

Query: 190 HEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLR 249
           H+VDMH+GC+F+HRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAF+LG APVY+AFLR
Sbjct: 165 HKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 224

Query: 250 FMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGN 309
           FMGDD +AK +SYSLEVGG GRK+IWQG+PRSIRDSHRKVRDS+DGLIIQR +ALF SG 
Sbjct: 225 FMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGG 284

Query: 310 EGMELKLKVTGRIWR 325
           E  ELKL+VTGRIW+
Sbjct: 285 ERKELKLRVTGRIWK 299

[Close]

BLAST of PI0016716 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.0e-123
Identity = 196/265 (73.96%), Postives = 228/265 (86.04%), Query Frame = 0


Query: 60  ASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 119
           A+  G   +   VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 120 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 179
           IRCLALEKVAESLELPC + +LGC ++FPYYSKLKHE  C +RPY+CPYAG EC VTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 180 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 239
           P LV HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 240 TAPVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 299
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 300 RKLALFCSGNEGMELKLKVTGRIWR 325
           R +ALF SG +  ELKL+VTGRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of PI0016716 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 433.0 bits (1112), Expect = 3.1e-120
Identity = 190/253 (75.10%), Postives = 220/253 (86.96%), Query Frame = 0


Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC N M+PPI+QC NGHTLCS+CK+RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 40  VYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAES 99

Query: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191
           LELPC Y NLGC+ +FPYYSKLKHE  C +RPY+CPYAG EC   GDI  LV HL+DDH+
Sbjct: 100 LELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHK 159

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG APVYMAFLRFM
Sbjct: 160 VDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFM 219

Query: 252 GDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD++DA+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +ALF SG + 
Sbjct: 220 GDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDK 279

Query: 312 MELKLKVTGRIWR 325
            ELKL+VTGRIW+
Sbjct: 280 KELKLRVTGRIWK 292

[Close]

BLAST of PI0016716 vs. ExPASy TrEMBL
Match: A0A1S3BMT4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 2.6e-194
Identity = 322/326 (98.77%), Postives = 323/326 (99.08%), Query Frame = 0


Query: 1   MSTTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKA 60
           MST IEFREKGMYQQQKSREEHASSAASFCKEVTEL L SIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDN+AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of PI0016716 vs. ExPASy TrEMBL
Match: A0A0A0KWV9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.3e-193
Identity = 321/326 (98.47%), Postives = 323/326 (99.08%), Query Frame = 0


Query: 1   MSTTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKA 60
           MST IEFREKGMYQQ KSREEHASSAASFCKEVTELRL SIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDD++AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of PI0016716 vs. ExPASy TrEMBL
Match: A0A5D3DDZ5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00330 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 9.5e-189
Identity = 312/315 (99.05%), Postives = 313/315 (99.37%), Query Frame = 0


Query: 12  MYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKASTGGFARSRND 71
           MYQQQKSREEHASSAASFCKEVTEL L SIDSQPATSRPEFKGVTFRKASTGGFARSRND
Sbjct: 1   MYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKASTGGFARSRND 60

Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES
Sbjct: 61  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 120

Query: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191
           LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE
Sbjct: 121 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 180

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 181 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240

Query: 252 GDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GDDN+AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG
Sbjct: 241 GDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 300

Query: 312 MELKLKVTGRIWRVR 327
           MELKLKVTGRIWRVR
Sbjct: 301 MELKLKVTGRIWRVR 315

[Close]

BLAST of PI0016716 vs. ExPASy TrEMBL
Match: A0A6J1DI06 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021266 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.6e-170
Identity = 285/323 (88.24%), Postives = 299/323 (92.57%), Query Frame = 0


Query: 5   IEFREKGMYQQQKSREEH-ASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKASTG 64
           +EFREK MYQQ+K REEH +SS ASFCKEVTELRL SIDSQ ATSRPEFK V+ RK+ST 
Sbjct: 4   VEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSST- 63

Query: 65  GFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCL 124
           GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGNIRCL
Sbjct: 64  GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCL 123

Query: 125 ALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLV 184
            LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP LV
Sbjct: 124 VLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLV 183

Query: 185 MHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPV 244
           MHLKDDHEVDMH+GCSFSHRYVKSNP+EVENATWMLTVFNCFGRQFCLHFEAFRLGTAPV
Sbjct: 184 MHLKDDHEVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPV 243

Query: 245 YMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLA 304
           YMAFLRFMGDD+DAK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R LA
Sbjct: 244 YMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLA 303

Query: 305 LFCSGNEGMELKLKVTGRIWRVR 327
           LFCSGN G ELKLKV GRIWRVR
Sbjct: 304 LFCSGNHGQELKLKVVGRIWRVR 325

[Close]

BLAST of PI0016716 vs. ExPASy TrEMBL
Match: A0A5C7IUW2 (RING-type E3 ubiquitin transferase OS=Acer yangbiense OX=1000413 GN=EZV62_001706 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 6.9e-139
Identity = 232/297 (78.11%), Postives = 256/297 (86.20%), Query Frame = 0


Query: 30  CKEVTELRLASIDSQPATSRPEFKGVTFRKAST--GGFARSRNDVHELLDCTVCMNLMHP 89
           CKE+ E R+A  D +  TS  E +G   RKA+T  GG   S NDVHELL+C VCMNLM+P
Sbjct: 8   CKEIIESRIAFADYETTTSNVELRGSPCRKATTGFGGNTTSTNDVHELLECPVCMNLMYP 67

Query: 90  PIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDMF 149
           PIYQC NGHT+CS+CK RV+N CPTCRHELGNIRCLALEKVAESLELPC YQ LGC D+F
Sbjct: 68  PIYQCPNGHTVCSNCKVRVRNSCPTCRHELGNIRCLALEKVAESLELPCRYQILGCHDIF 127

Query: 150 PYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVKS 209
           PYY KLKHEKNCKYRPYNCPYAG EC VTGDIP LVMHLK+DH+VDMHDG +F+HRYVK+
Sbjct: 128 PYYGKLKHEKNCKYRPYNCPYAGAECSVTGDIPFLVMHLKNDHKVDMHDGSTFNHRYVKA 187

Query: 210 NPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNDAKQFSYSLEVG 269
           N QEVENATWMLTVFNCFGRQFCLHFEAF LG+APVYMAFLRFMGD+++A+QFSY+LEVG
Sbjct: 188 NAQEVENATWMLTVFNCFGRQFCLHFEAFHLGSAPVYMAFLRFMGDEDEARQFSYTLEVG 247

Query: 270 GNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           GNGRKL WQGIPRSIRDSHRKVRDS DGLIIQR LALF SG +  ELKLKV GRIW+
Sbjct: 248 GNGRKLTWQGIPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDKQELKLKVAGRIWK 304

[Close]

BLAST of PI0016716 vs. NCBI nr
Match: XP_008449780.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 687.6 bits (1773), Expect = 5.3e-194
Identity = 322/326 (98.77%), Postives = 323/326 (99.08%), Query Frame = 0


Query: 1   MSTTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKA 60
           MST IEFREKGMYQQQKSREEHASSAASFCKEVTEL L SIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDN+AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of PI0016716 vs. NCBI nr
Match: XP_031740164.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetical protein Csa_018085 [Cucumis sativus])

HSP 1 Score: 685.3 bits (1767), Expect = 2.6e-193
Identity = 321/326 (98.47%), Postives = 323/326 (99.08%), Query Frame = 0


Query: 1   MSTTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKA 60
           MST IEFREKGMYQQ KSREEHASSAASFCKEVTELRL SIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDD++AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of PI0016716 vs. NCBI nr
Match: XP_038900383.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 674.5 bits (1739), Expect = 4.7e-190
Identity = 314/323 (97.21%), Postives = 318/323 (98.45%), Query Frame = 0


Query: 3   TTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKAST 62
           TT+EFREKGMYQQQKSREEHA SAASFCKEVTELRL SIDSQ ATSRPEFKG+TFRKAST
Sbjct: 14  TTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAST 73

Query: 63  GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRC 122
           GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNIRC
Sbjct: 74  GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRC 133

Query: 123 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSL 182
           LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP L
Sbjct: 134 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYL 193

Query: 183 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 242
           VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP
Sbjct: 194 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 253

Query: 243 VYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 302
           VYMAFLRFMGDD+DAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL
Sbjct: 254 VYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 313

Query: 303 ALFCSGNEGMELKLKVTGRIWRV 326
           ALFCSGNEGMELKLKVTGRIWRV
Sbjct: 314 ALFCSGNEGMELKLKVTGRIWRV 336

[Close]

BLAST of PI0016716 vs. NCBI nr
Match: XP_038900384.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_038900386.1 E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 674.5 bits (1739), Expect = 4.7e-190
Identity = 314/323 (97.21%), Postives = 318/323 (98.45%), Query Frame = 0


Query: 3   TTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKAST 62
           TT+EFREKGMYQQQKSREEHA SAASFCKEVTELRL SIDSQ ATSRPEFKG+TFRKAST
Sbjct: 2   TTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAST 61

Query: 63  GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRC 122
           GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNIRC
Sbjct: 62  GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRC 121

Query: 123 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSL 182
           LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP L
Sbjct: 122 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYL 181

Query: 183 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 242
           VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP
Sbjct: 182 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 241

Query: 243 VYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 302
           VYMAFLRFMGDD+DAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL
Sbjct: 242 VYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 301

Query: 303 ALFCSGNEGMELKLKVTGRIWRV 326
           ALFCSGNEGMELKLKVTGRIWRV
Sbjct: 302 ALFCSGNEGMELKLKVTGRIWRV 324

[Close]

BLAST of PI0016716 vs. NCBI nr
Match: XP_038900382.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 674.5 bits (1739), Expect = 4.7e-190
Identity = 314/323 (97.21%), Postives = 318/323 (98.45%), Query Frame = 0


Query: 3   TTIEFREKGMYQQQKSREEHASSAASFCKEVTELRLASIDSQPATSRPEFKGVTFRKAST 62
           TT+EFREKGMYQQQKSREEHA SAASFCKEVTELRL SIDSQ ATSRPEFKG+TFRKAST
Sbjct: 14  TTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAST 73

Query: 63  GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRC 122
           GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNIRC
Sbjct: 74  GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRC 133

Query: 123 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSL 182
           LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP L
Sbjct: 134 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYL 193

Query: 183 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 242
           VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP
Sbjct: 194 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 253

Query: 243 VYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 302
           VYMAFLRFMGDD+DAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL
Sbjct: 254 VYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 313

Query: 303 ALFCSGNEGMELKLKVTGRIWRV 326
           ALFCSGNEGMELKLKVTGRIWRV
Sbjct: 314 ALFCSGNEGMELKLKVTGRIWRV 336

[Close]

BLAST of PI0016716 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 474.2 bits (1219), Expect = 8.6e-134
Identity = 218/301 (72.43%), Postives = 250/301 (83.06%), Query Frame = 0


Query: 28  SFCKEVTELRLASIDSQPATSRPEFKGVTFRKASTGGFAR----SRNDVHELLDCTVCMN 87
           S  KEV E     +D +  T++ E       K  + G  +    S N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 88  LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGC 147
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 148 IDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHR 207
            D+FPYYSKLKHE++C++RPY CPYAG EC VTGDIP+LV+HLKDDH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 208 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNDAKQFSYS 267
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+N+AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 268 LEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIW 325
           LEVG +GRKL WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG +  ELKL+VTGRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

[Close]

BLAST of PI0016716 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 464.9 bits (1195), Expect = 5.2e-131
Identity = 216/290 (74.48%), Postives = 248/290 (85.52%), Query Frame = 0


Query: 35  ELRLASIDSQPATSRPEFKGVTFRKASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCAN 94
           E+++A ++   A S+P   G      S G F  S N V+ELL+C VC NLM+PPI+QC N
Sbjct: 22  EVKMAKVE---ANSKPTKSG----SGSIGKF-HSSNGVYELLECPVCTNLMYPPIHQCPN 81

Query: 95  GHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLK 154
           GHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC D+FPYYSKLK
Sbjct: 82  GHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLK 141

Query: 155 HEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVEN 214
           HE++C++R Y+CPYAG EC VTGDIP+LV HLKDDH+VDMHDGC+F+HRYVKSNP EVEN
Sbjct: 142 HEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVEN 201

Query: 215 ATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLI 274
           ATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+N+AK+FSYSLEVG + RKL 
Sbjct: 202 ATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLT 261

Query: 275 WQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG++  ELKL+VTGRIW+
Sbjct: 262 WQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of PI0016716 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 443.0 bits (1138), Expect = 2.1e-124
Identity = 196/265 (73.96%), Postives = 228/265 (86.04%), Query Frame = 0


Query: 60  ASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 119
           A+  G   +   VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 120 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 179
           IRCLALEKVAESLELPC + +LGC ++FPYYSKLKHE  C +RPY+CPYAG EC VTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 180 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 239
           P LV HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 240 TAPVYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 299
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 300 RKLALFCSGNEGMELKLKVTGRIWR 325
           R +ALF SG +  ELKL+VTGRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of PI0016716 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 424.5 bits (1090), Expect = 7.8e-119
Identity = 188/253 (74.31%), Postives = 219/253 (86.56%), Query Frame = 0


Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC   M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 58  VYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAES 117

Query: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191
           LELPC + NLGC ++FPYYSKLKHE  C +RPY+CPYAG EC + GDIP LV HL+DDH+
Sbjct: 118 LELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHK 177

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRFM
Sbjct: 178 VDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFM 237

Query: 252 GDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD+ DA+ +SYSLEVGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQR +ALF SG + 
Sbjct: 238 GDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDR 297

Query: 312 MELKLKVTGRIWR 325
            ELKL+VTG+IW+
Sbjct: 298 KELKLRVTGKIWK 310

[Close]

BLAST of PI0016716 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.6e-95
Identity = 152/202 (75.25%), Postives = 176/202 (87.13%), Query Frame = 0


Query: 123 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSL 182
           +ALEKVAESLELPC Y NLGC+ +FPYYSKLKHE  C +RPY+CPYAG EC   GDI  L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 183 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 242
           V HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 243 VYMAFLRFMGDDNDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 302
           VYMAFLRFMGD++DA+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 303 ALFCSGNEGMELKLKVTGRIWR 325
           ALF SG +  ELKL+VTGRIW+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWK 216

The following BLAST results are available for this feature:

BLAST of PI0016716 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2P4	1.2e-132	72.43	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=...	[more]
P93748	7.4e-130	74.48	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q10L91	4.6e-124	76.86	E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS...	[more]
Q84JL3	3.0e-123	73.96	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=...	[more]
Q8S3N1	3.1e-120	75.10	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=...	[more]

BLAST of PI0016716 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BMT4	2.6e-194	98.77	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 ...	[more]
A0A0A0KWV9	1.3e-193	98.47	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE...	[more]
A0A5D3DDZ5	9.5e-189	99.05	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1DI06	7.6e-170	88.24	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102126...	[more]
A0A5C7IUW2	6.9e-139	78.11	RING-type E3 ubiquitin transferase OS=Acer yangbiense OX=1000413 GN=EZV62_001706...	[more]

BLAST of PI0016716 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008449780.1	5.3e-194	98.77	PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo]	[more]
XP_031740164.1	2.6e-193	98.47	E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetic...	[more]
XP_038900383.1	4.7e-190	97.21	E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida]	[more]
XP_038900384.1	4.7e-190	97.21	E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_03890...	[more]
XP_038900382.1	4.7e-190	97.21	E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida]	[more]

BLAST of PI0016716 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58040.1	8.6e-134	72.43	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	5.2e-131	74.48	Protein with RING/U-box and TRAF-like domains	[more]
AT3G61790.1	2.1e-124	73.96	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	7.8e-119	74.31	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	1.6e-95	75.25	TRAF-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008974	TRAF-like	GENE3D	2.60.210.10	Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A	coord: 187..319 e-value: 2.5E-36 score: 126.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 8..130 e-value: 3.3E-6 score: 28.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 131..186 e-value: 3.8E-16 score: 61.1
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 120..319 e-value: 2.7E-79 score: 265.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR10315:SF123	E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKE	coord: 27..324
None	No IPR available	PANTHER	PTHR10315	E3 UBIQUITIN PROTEIN LIGASE SIAH	coord: 27..324
None	No IPR available	CDD	cd16571	RING-HC_SIAHs	coord: 76..114 e-value: 8.45687E-13 score: 59.991
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 131..322
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 60..118
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 131..191 score: 13.758881
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 78..114 score: 9.214112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0016716.1	PI0016716.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding