PI0016370 (gene) Melon (PI 482460) v1

Overview
NamePI0016370
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProlyl-tRNA synthetase
Locationchr11: 7983221 .. 7983622 (+)
RNA-Seq ExpressionPI0016370
SyntenyPI0016370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATAAGTTTTCTGAAATCTGAAGTATTTAATATATTTGTTGTATTCCAACCTCTATCATTTACTAGTGTATTTCTAAAGAATGATGGTTTCATTGTTTTGTTCCTCCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTTACTAAATCCATGTCCAAATCTGTATTCATTTTACATGTTAACGAGTTTATATCAACTTGTGGGATCTTGGGAAACTTAATGGCAACTCTTTTGGTTCCTTGAATTTACTATGATGTTTGTGTTCAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGGAGCTACTAA

mRNA sequence

ATGGAGATAAGTTTTCTGAAATCTGAAGTATTTAATATATTTGTTGTATTCCAACCTCTATCATTTACTAGTGTATTTCTAAAGAATGATGGTTTCATTGTTTTGTTCCTCCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGGAGCTACTAA

Coding sequence (CDS)

ATGGAGATAAGTTTTCTGAAATCTGAAGTATTTAATATATTTGTTGTATTCCAACCTCTATCATTTACTAGTGTATTTCTAAAGAATGATGGTTTCATTGTTTTGTTCCTCCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGGAGCTACTAA

Protein sequence

MEISFLKSEVFNIFVVFQPLSFTSVFLKNDGFIVLFLQEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Homology
[Close]
BLAST of PI0016370 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 9.1e-22
Identity = 45/53 (84.91%), Postives = 49/53 (92.45%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 478 EEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]
BLAST of PI0016370 vs. ExPASy Swiss-Prot
Match: Q8I5R7 (Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.6e-08
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 0 

Query: 38  QEVEKDVK----TRTKGEV---GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +E++K+ +     +T  E    GA K LC P +QPP+P   KCF SGKPAK+W  +GRSY
Sbjct: 687 EEIKKETQRLSLNQTNSETTLSGAMKPLCIPLDQPPMPPNMKCFWSGKPAKRWCLFGRSY 746

[Close]
BLAST of PI0016370 vs. ExPASy TrEMBL
Match: A0A1S4DS87 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-22
Identity = 51/53 (96.23%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 459 EEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 511

[Close]
BLAST of PI0016370 vs. ExPASy TrEMBL
Match: A0A5A7U355 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00130 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-22
Identity = 51/53 (96.23%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 460 EEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 512

[Close]
BLAST of PI0016370 vs. ExPASy TrEMBL
Match: A0A1S3CR78 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-22
Identity = 51/53 (96.23%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 409 EEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 461

[Close]
BLAST of PI0016370 vs. ExPASy TrEMBL
Match: A0A6J1JLY7 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.5e-22
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 459 EEVEKDVKTRTKGEMGAAKTLCSPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

[Close]
BLAST of PI0016370 vs. ExPASy TrEMBL
Match: A0A6J1IRP0 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.5e-22
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 458 EEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]
BLAST of PI0016370 vs. NCBI nr
Match: KGN50982.2 (hypothetical protein Csa_004679 [Cucumis sativus])

HSP 1 Score: 116.7 bits (291), Expect = 1.0e-22
Identity = 52/53 (98.11%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           QEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 285 QEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 337

[Close]
BLAST of PI0016370 vs. NCBI nr
Match: XP_008466343.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo])

HSP 1 Score: 115.5 bits (288), Expect = 2.3e-22
Identity = 51/53 (96.23%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 409 EEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 461

[Close]
BLAST of PI0016370 vs. NCBI nr
Match: XP_008466310.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_008466350.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_016898851.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo])

HSP 1 Score: 115.5 bits (288), Expect = 2.3e-22
Identity = 51/53 (96.23%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 459 EEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 511

[Close]
BLAST of PI0016370 vs. NCBI nr
Match: KAA0050272.1 (proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 115.5 bits (288), Expect = 2.3e-22
Identity = 51/53 (96.23%), Postives = 53/53 (100.00%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 460 EEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 512

[Close]
BLAST of PI0016370 vs. NCBI nr
Match: XP_022979761.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 112.5 bits (280), Expect = 2.0e-21
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVEKDVKTRTKGE+GAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 458 EEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]
BLAST of PI0016370 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 6.5e-23
Identity = 45/53 (84.91%), Postives = 49/53 (92.45%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 478 EEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]
BLAST of PI0016370 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 6.5e-23
Identity = 45/53 (84.91%), Postives = 49/53 (92.45%), Query Frame = 0 

Query: 38  QEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           +EVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 478 EEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]
BLAST of PI0016370 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 85.5 bits (210), Expect = 2.4e-17
Identity = 41/57 (71.93%), Postives = 44/57 (77.19%), Query Frame = 0 

Query: 39  EVEKDVKTRTKGEV-----GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 91
           EVEKDVK RT+G+      G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 253 EVEKDVKRRTRGDETGGGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309

The following BLAST results are available for this feature:
BLAST of PI0016370 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M1R29.1e-2284.91Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P... [more]
Q8I5R72.6e-0846.67Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE... [more]
BLAST of PI0016370 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S4DS871.1e-2296.23Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1[more]
A0A5A7U3551.1e-2296.23Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3CR781.1e-2296.23Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1[more]
A0A6J1JLY79.5e-2294.34Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1[more]
A0A6J1IRP09.5e-2294.34Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1[more]
BLAST of PI0016370 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN50982.21.0e-2298.11hypothetical protein Csa_004679 [Cucumis sativus][more]
XP_008466343.12.3e-2296.23PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo][more]
XP_008466310.12.3e-2296.23PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_00846... [more]
KAA0050272.12.3e-2296.23proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase... [more]
XP_022979761.12.0e-2194.34proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima][more]
BLAST of PI0016370 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G62120.16.5e-2384.91Class II aaRS and biotin synthetases superfamily protein [more]
AT3G62120.26.5e-2384.91Class II aaRS and biotin synthetases superfamily protein [more]
AT5G10880.12.4e-1771.93tRNA synthetase-related / tRNA ligase-related [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016061Proline-tRNA ligase, class II, C-terminalSMARTSM00946ProRS_C_1_2coord: 22..90
e-value: 3.1E-11
score: 53.3
IPR016061Proline-tRNA ligase, class II, C-terminalPFAMPF09180ProRS-C_1coord: 38..90
e-value: 1.0E-16
score: 61.0
IPR017449Prolyl-tRNA synthetase, class IIGENE3D3.30.110.30coord: 28..90
e-value: 4.3E-16
score: 60.9
IPR017449Prolyl-tRNA synthetase, class IISUPERFAMILY64586C-terminal domain of ProRScoord: 39..90
NoneNo IPR availablePANTHERPTHR43382:SF4PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKEcoord: 38..90
IPR004499Proline-tRNA ligase, class IIa, archaeal-typePANTHERPTHR43382PROLYL-TRNA SYNTHETASEcoord: 38..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016370.1PI0016370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006809 nitric oxide biosynthetic process
biological_process GO:0006433 prolyl-tRNA aminoacylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0071949 FAD binding
molecular_function GO:0020037 heme binding
molecular_function GO:0030151 molybdenum ion binding
molecular_function GO:0043546 molybdopterin cofactor binding
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0050464 nitrate reductase (NADPH) activity
molecular_function GO:0004827 proline-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding